##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248460_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22995697
Sequences flagged as poor quality	0
Sequence length	100
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.90150135479694	31.0	28.0	33.0	22.0	34.0
2	29.455631025230502	31.0	29.0	33.0	23.0	34.0
3	29.802734050635646	31.0	30.0	34.0	25.0	34.0
4	33.248873691456275	35.0	33.0	37.0	27.0	37.0
5	33.02638828472997	35.0	33.0	37.0	26.0	37.0
6	32.83435505346935	35.0	32.0	37.0	26.0	37.0
7	32.9444187319045	35.0	32.0	37.0	27.0	37.0
8	32.778650805844244	35.0	32.0	37.0	25.0	37.0
9	33.98717612255893	37.0	32.0	39.0	25.0	39.0
10-11	34.26413258967537	37.0	33.0	39.0	27.0	39.0
12-13	34.162471613711034	37.0	32.5	39.0	27.0	39.0
14-15	34.884434596611705	37.0	32.0	40.0	27.0	41.0
16-17	34.683349041344556	37.0	32.0	40.0	27.0	41.0
18-19	34.18851818233646	36.5	32.0	39.5	25.5	41.0
20-21	33.8222230445983	35.5	32.0	39.0	25.0	40.5
22-23	33.267767095731	35.0	31.5	38.5	25.0	40.0
24-25	32.761530189756805	35.0	30.5	38.0	24.0	40.0
26-27	32.29499879912316	35.0	30.0	37.5	22.5	40.0
28-29	32.1137106433434	35.0	30.0	37.0	22.5	40.0
30-31	31.98854394367781	35.0	30.0	37.0	22.0	40.0
32-33	31.788734257543922	34.5	30.0	37.0	22.0	40.0
34-35	31.620836780898617	34.0	28.5	37.0	21.0	40.0
36-37	31.428644628601603	34.0	28.0	37.0	21.0	39.5
38-39	31.21696430858347	34.0	27.5	37.0	20.0	39.0
40-41	30.951496316897895	33.0	26.5	37.0	20.0	39.0
42-43	30.695311214093664	33.0	26.0	36.0	18.5	39.0
44-45	30.442749832718704	33.0	25.5	35.5	18.5	39.0
46-47	30.294226415489817	33.0	25.0	35.0	18.5	39.0
48-49	30.084790798034952	33.0	25.5	35.0	18.0	39.0
50-51	29.875841380237354	33.0	26.0	35.0	18.0	39.0
52-53	29.599016981307415	33.0	26.0	35.0	17.5	38.0
54-55	29.39177168667686	32.0	26.0	35.0	16.0	38.0
56-57	29.17574431425149	32.0	26.0	35.0	15.0	37.0
58-59	28.89682404495067	31.0	26.0	35.0	15.0	37.0
60-61	28.641160626703336	31.0	26.0	35.0	14.0	36.0
62-63	28.6023163594476	31.0	25.5	35.0	15.5	36.0
64-65	28.87150809127464	31.0	26.0	35.0	17.5	35.0
66-67	29.339583836054196	32.0	26.0	35.0	19.5	35.0
68-69	29.379364191483305	32.0	26.0	35.0	19.5	35.0
70-71	29.71094683496656	32.5	27.5	35.0	20.0	35.0
72-73	29.737392173848875	33.0	27.5	35.0	20.0	35.0
74-75	29.662816634781716	33.0	27.0	35.0	20.0	35.0
76-77	29.547123359644196	33.0	27.0	35.0	19.5	35.0
78-79	29.417168372848188	33.0	27.0	35.0	19.0	35.0
80-81	29.30892710057886	33.0	27.0	35.0	18.0	35.0
82-83	29.193617788580184	33.0	27.0	35.0	17.5	35.0
84-85	29.07225145208688	33.0	27.0	35.0	17.0	35.0
86-87	28.877720992757908	33.0	27.0	35.0	15.0	35.0
88-89	28.67762305704411	33.0	26.0	35.0	13.0	35.0
90-91	28.408389860937895	32.5	25.5	35.0	10.0	35.0
92-93	28.132155050573157	32.0	25.0	35.0	10.0	35.0
94-95	27.77590244383547	32.0	25.0	35.0	8.0	35.0
96-97	27.376755007686874	32.0	24.5	35.0	7.5	35.0
98-99	26.906041660750702	31.5	23.5	35.0	7.0	35.0
100	25.568154859580904	30.0	19.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6553084829324867
1101	2	0.5864380437664778
1101	3	0.5679082727445746
1101	4	0.5289643037239244
1101	5	0.4327269679557588
1101	6	0.29765234673588736
1101	7	0.3592517865329157
1101	8	0.48308251614722764
1101	9	0.4463181397401641
1101	10-11	0.5028775433976946
1101	12-13	0.6111131479322367
1101	14-15	0.7490924261318526
1101	16-17	0.711457807753689
1101	18-19	0.7867863410003011
1101	20-21	0.645242063065524
1101	22-23	0.5806984981027199
1101	24-25	0.5479338655860673
1101	26-27	0.6482332124433015
1101	28-29	0.4909268836091627
1101	30-31	0.4622063061395565
1101	32-33	0.46477631532196284
1101	34-35	0.4654998748125756
1101	36-37	0.46438470399085574
1101	38-39	0.3297688689700493
1101	40-41	0.41244142083771607
1101	42-43	0.3676218867101291
1101	44-45	0.42942145290999534
1101	46-47	0.41219738257253624
1101	48-49	0.3304108701408843
1101	50-51	0.31500030650777333
1101	52-53	0.33309733665873864
1101	54-55	0.2396104060153057
1101	56-57	0.2547949024726215
1101	58-59	0.26907281536474414
1101	60-61	0.27575825311301116
1101	62-63	0.34750131344135227
1101	64-65	0.3669607027512427
1101	66-67	0.3966211385116942
1101	68-69	0.4022969301692072
1101	70-71	0.42676986946990425
1101	72-73	0.43536998159567375
1101	74-75	0.4122932228430507
1101	76-77	0.4647955522918856
1101	78-79	0.4699814433309015
1101	80-81	0.4146705793196759
1101	82-83	0.3659742469679479
1101	84-85	0.3546626967673596
1101	86-87	0.30043302638819114
1101	88-89	0.29024157798544437
1101	90-91	0.327495099269548
1101	92-93	0.2592975830319908
1101	94-95	0.31580406002765216
1101	96-97	0.29424785960189936
1101	98-99	0.2539526610797971
1101	100	0.19760043184974307
1102	1	0.4417071337785998
1102	2	0.43784490543982457
1102	3	0.40129666147852205
1102	4	0.3721778804392173
1102	5	0.33727837004713024
1102	6	0.26320827305742966
1102	7	0.30048899135368856
1102	8	0.3200567444683955
1102	9	0.3090067558011569
1102	10-11	0.3090805602799165
1102	12-13	0.37864770924251445
1102	14-15	0.5244858070520806
1102	16-17	0.5632419251894021
1102	18-19	0.628064536056435
1102	20-21	0.45559597403679675
1102	22-23	0.3603649856240665
1102	24-25	0.34932453750933945
1102	26-27	0.43085659593059233
1102	28-29	0.2701025670945967
1102	30-31	0.269281515764483
1102	32-33	0.23807374456000474
1102	34-35	0.23978638450299528
1102	36-37	0.2857527283572949
1102	38-39	0.25957034900791953
1102	40-41	0.19581642422461698
1102	42-43	0.2214146720413126
1102	44-45	0.2334781808573574
1102	46-47	0.2559971497238003
1102	48-49	0.24831383401153673
1102	50-51	0.24224343066014242
1102	52-53	0.23719605874647343
1102	54-55	0.19069456604806234
1102	56-57	0.10702452110824368
1102	58-59	0.1433736458591568
1102	60-61	0.18338382499729278
1102	62-63	0.1530115427893861
1102	64-65	0.24689024774407642
1102	66-67	0.3109041781030548
1102	68-69	0.3030125221323452
1102	70-71	0.3178627340122553
1102	72-73	0.3137261745862183
1102	74-75	0.28918664229895086
1102	76-77	0.29355579529948983
1102	78-79	0.2662393934425289
1102	80-81	0.23272142144148233
1102	82-83	0.22853331641526253
1102	84-85	0.19290072481896203
1102	86-87	0.20949897274815044
1102	88-89	0.21108099913279332
1102	90-91	0.20960844614303298
1102	92-93	0.14375258941477753
1102	94-95	0.16643821798776415
1102	96-97	0.1232616035179035
1102	98-99	0.11838118079341342
1102	100	0.014432668581360275
1103	1	0.2679032410744462
1103	2	0.24134207487706405
1103	3	0.24961302207872293
1103	4	0.26997123118084687
1103	5	0.27681505263717554
1103	6	0.16530403782383019
1103	7	0.2901883036700923
1103	8	0.2952999210332692
1103	9	0.32900127497833864
1103	10-11	0.27891096931639936
1103	12-13	0.35013878851979996
1103	14-15	0.4382799572516518
1103	16-17	0.47072337591226443
1103	18-19	0.5358054531584315
1103	20-21	0.3996650514832538
1103	22-23	0.31228976575494727
1103	24-25	0.2761798928733654
1103	26-27	0.3948004379508063
1103	28-29	0.19848685443628256
1103	30-31	0.09849897365010207
1103	32-33	0.056626408794670624
1103	34-35	0.11424065023418706
1103	36-37	0.06434992186537869
1103	38-39	0.091035662878582
1103	40-41	0.14636988393725758
1103	42-43	0.09197883951080854
1103	44-45	0.05394857170004741
1103	46-47	0.06991856635936244
1103	48-49	-0.001762925949261529
1103	50-51	0.014091034453009854
1103	52-53	0.03120588304960492
1103	54-55	0.04188670215636492
1103	56-57	0.04406665072511018
1103	58-59	0.0398386685615435
1103	60-61	0.07556152202957378
1103	62-63	0.07421050462889411
1103	64-65	0.17901225530168574
1103	66-67	0.15240179356805328
1103	68-69	0.17354845408140207
1103	70-71	0.17992590716798063
1103	72-73	0.16626603754387403
1103	74-75	0.13134633697991305
1103	76-77	0.14880496788948605
1103	78-79	0.09230335904598164
1103	80-81	0.13941354472204637
1103	82-83	0.04894326731831811
1103	84-85	0.017177736764889318
1103	86-87	0.09141393915560414
1103	88-89	0.06832960080298633
1103	90-91	0.06534628323069924
1103	92-93	0.06593743224554771
1103	94-95	0.05131070462993392
1103	96-97	0.020455474486688274
1103	98-99	0.020506906972737937
1103	100	0.005306076726366626
1104	1	0.18387313733373034
1104	2	0.19829922550906076
1104	3	0.1072084931390691
1104	4	0.19760524720311423
1104	5	0.16095855703682105
1104	6	0.1382090182535194
1104	7	0.10909274244581724
1104	8	0.2428989478948438
1104	9	0.07082419818404162
1104	10-11	0.1072794498247518
1104	12-13	0.19764819300593217
1104	14-15	0.3474538056695593
1104	16-17	0.3828444826905937
1104	18-19	0.3883371884210334
1104	20-21	0.28061910704550286
1104	22-23	0.17219244664520517
1104	24-25	0.21353309014008204
1104	26-27	0.2615852945470536
1104	28-29	0.11599264757675343
1104	30-31	0.14482113843490652
1104	32-33	0.16012213632663475
1104	34-35	0.1663865700504381
1104	36-37	0.06215538487082384
1104	38-39	0.10896366360180565
1104	40-41	0.07700630244768547
1104	42-43	0.08435602191613256
1104	44-45	0.05237173905225845
1104	46-47	0.041856755271911794
1104	48-49	0.12863821880230475
1104	50-51	0.10344844859544722
1104	52-53	0.09258818760909904
1104	54-55	0.04164915634298083
1104	56-57	0.0749733843477145
1104	58-59	0.06754964761736915
1104	60-61	0.07799670766432953
1104	62-63	0.040054662183163
1104	64-65	0.17829214186846443
1104	66-67	0.20982927720136146
1104	68-69	0.14237806110168094
1104	70-71	0.11404644729735125
1104	72-73	0.15378255650545114
1104	74-75	0.14618992194770897
1104	76-77	0.1546139442717056
1104	78-79	0.13405619044727857
1104	80-81	0.08719801624216217
1104	82-83	0.09388759599731245
1104	84-85	0.10367939529286119
1104	86-87	0.03840030711581832
1104	88-89	0.038921273007336055
1104	90-91	0.04889839314814637
1104	92-93	0.044878198036613526
1104	94-95	0.12647156465308385
1104	96-97	0.1386685866880626
1104	98-99	0.17598761193881884
1104	100	0.15301105746250343
1105	1	0.1910895946261313
1105	2	0.18499359697030116
1105	3	0.18824754763353724
1105	4	0.17561035818157933
1105	5	0.12843963980842688
1105	6	0.0938845290100403
1105	7	0.08890896312153984
1105	8	0.22283207519436132
1105	9	0.2235410010809673
1105	10-11	0.23014619702628636
1105	12-13	0.2543905076515074
1105	14-15	0.34935764696781746
1105	16-17	0.32093488890196653
1105	18-19	0.4125298000025097
1105	20-21	0.2673437801138192
1105	22-23	0.16796101940683883
1105	24-25	0.12668447456972132
1105	26-27	0.19142160524926055
1105	28-29	0.0993679586162699
1105	30-31	0.04024904999146628
1105	32-33	0.04799050460179899
1105	34-35	0.07351323752172334
1105	36-37	0.007287670304297222
1105	38-39	-0.03394948580839241
1105	40-41	0.011889566032074583
1105	42-43	-0.05205578303148428
1105	44-45	-0.029007196602314878
1105	46-47	0.03664965105553364
1105	48-49	-0.04201484358873486
1105	50-51	-0.027778053346111165
1105	52-53	-0.0893002475733482
1105	54-55	-0.059033728887733616
1105	56-57	-0.06851125131484892
1105	58-59	-0.06281888585985129
1105	60-61	-0.05591504039552575
1105	62-63	-0.009240767881937728
1105	64-65	0.09545700332719775
1105	66-67	0.08431680403645458
1105	68-69	0.07934255203450036
1105	70-71	0.04051008655212485
1105	72-73	0.0893385419807764
1105	74-75	0.10737254567677113
1105	76-77	0.06715300249801004
1105	78-79	0.12078808143467512
1105	80-81	0.10515027348479578
1105	82-83	0.05805129949429855
1105	84-85	0.026339925727082658
1105	86-87	0.011155440530817629
1105	88-89	0.09753897778916709
1105	90-91	0.08730661443989973
1105	92-93	0.10319567470307689
1105	94-95	0.06521721410469183
1105	96-97	0.07711084597501383
1105	98-99	0.08199838001824844
1105	100	0.15958690941815945
1106	1	3.94567864574924E-4
1106	2	0.06198694895427792
1106	3	0.1234234773275773
1106	4	0.09430354224233639
1106	5	0.16487530683441065
1106	6	0.004762016077471287
1106	7	0.0638412702965141
1106	8	0.24507959717291072
1106	9	0.15725285443091508
1106	10-11	0.11939582286996853
1106	12-13	0.18880104682612142
1106	14-15	0.30463597384965624
1106	16-17	0.1856128365690708
1106	18-19	0.20978046331391909
1106	20-21	0.13828534687465321
1106	22-23	0.08302537173432256
1106	24-25	0.07584723842048646
1106	26-27	0.1655626441339848
1106	28-29	0.04853008253837743
1106	30-31	0.010746182102217006
1106	32-33	0.030866726170096115
1106	34-35	0.03825175478610987
1106	36-37	0.05568144989420176
1106	38-39	0.02387878473401628
1106	40-41	-0.07269245374131827
1106	42-43	-0.01273185307796254
1106	44-45	-0.05813321907149671
1106	46-47	-9.920393342710554E-4
1106	48-49	-0.07783759796008738
1106	50-51	-0.07459538704264901
1106	52-53	-0.04826323646708275
1106	54-55	-0.0456129369824545
1106	56-57	-0.09074909743219806
1106	58-59	-0.07370658136299468
1106	60-61	-0.022500322707067255
1106	62-63	-0.03150837243037685
1106	64-65	0.06413528159613335
1106	66-67	0.007745550220001007
1106	68-69	-0.00437239937443934
1106	70-71	0.03124163348276099
1106	72-73	0.04169508661177801
1106	74-75	0.0650656407314898
1106	76-77	0.06612174959571249
1106	78-79	0.045158598037229325
1106	80-81	0.038892719271220955
1106	82-83	-0.038785732640089776
1106	84-85	-0.018133518015112315
1106	86-87	0.016411352075497376
1106	88-89	0.04095903321698913
1106	90-91	0.02232022430624525
1106	92-93	-0.0064579255493733
1106	94-95	-0.002085929336431036
1106	96-97	-0.016864927871566238
1106	98-99	-0.006124163081882017
1106	100	-0.0590801840867492
1107	1	0.022075363494860056
1107	2	0.07290350388542066
1107	3	0.12093983597420888
1107	4	0.09810539734235846
1107	5	0.09482118113145788
1107	6	0.01297814227277172
1107	7	0.001336778895627333
1107	8	0.17068036883950555
1107	9	0.17597976771371293
1107	10-11	0.10210145661469028
1107	12-13	0.15900221480237064
1107	14-15	0.23072686945214116
1107	16-17	0.21480754578565353
1107	18-19	0.2803544889863687
1107	20-21	0.13844408957933751
1107	22-23	0.08673253629751088
1107	24-25	0.0642477520546052
1107	26-27	0.15789022549449783
1107	28-29	-0.012656957298119664
1107	30-31	-0.03396923413133379
1107	32-33	-0.04935869383468372
1107	34-35	-0.008214728517042857
1107	36-37	-0.1235961968050141
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2216	38-39	0.9998881450777972
2216	40-41	1.042583341838018
2216	42-43	1.003837244757534
2216	44-45	1.0268444063574051
2216	46-47	0.9552614842824241
2216	48-49	0.9373644398231527
2216	50-51	0.9476369596198708
2216	52-53	0.9211099670878831
2216	54-55	0.8997915059745836
2216	56-57	0.9257122998198035
2216	58-59	0.8903007618531795
2216	60-61	0.8470597142471199
2216	62-63	0.7052663829040213
2216	64-65	0.5943926287942887
2216	66-67	0.6347234047140198
2216	68-69	0.6268906668748784
2216	70-71	0.54648860723373
2216	72-73	0.5793689548399392
2216	74-75	0.5383924283378327
2216	76-77	0.5204854751033174
2216	78-79	0.48263286693977747
2216	80-81	0.4671298645138826
2216	82-83	0.46015302711357364
2216	84-85	0.4425224666879295
2216	86-87	0.38332386209574665
2216	88-89	0.2659189740480379
2216	90-91	0.24523593239765162
2216	92-93	0.1888930926128225
2216	94-95	0.1518580235614948
2216	96-97	0.15439884354515243
2216	98-99	0.1500211637687876
2216	100	0.3498454938900508
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	436.0
6	4931.0
7	14552.0
8	25284.0
9	40109.0
10	59372.0
11	75394.0
12	82688.0
13	87955.0
14	96691.0
15	111414.0
16	130388.0
17	155491.0
18	187220.0
19	219663.0
20	253962.0
21	289016.0
22	326258.0
23	378290.0
24	499635.0
25	585973.0
26	671501.0
27	1068636.0
28	978901.0
29	1531185.0
30	1515746.0
31	1804962.0
32	2221819.0
33	3168344.0
34	2343005.0
35	3695643.0
36	300504.0
37	60415.0
38	8981.0
39	1333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.842323430951662	32.43470842808524	25.493652497290892	26.2293156436722
2	13.086756543695724	35.447965451429596	27.09041585752524	24.37486214734944
3	11.588242351717518	38.41727390824692	24.13416570514436	25.860318034891204
4	10.239762320678352	38.329369547076	24.03850025809184	27.392367874153813
5	10.182675609700732	37.78240261865452	25.053547139790844	26.9813746318539
6	10.186601432433207	34.62432123714276	26.444138657767148	28.74493867265689
7	14.137031810777467	39.46813614738444	21.93622572083812	24.45860632099997
8	12.21639422366715	36.59935595776897	19.72298991415655	31.461259904407335
9	9.811118140928714	35.706462822153206	20.55411932067117	33.92829971624691
10-11	14.194801314350244	49.788569139696	19.25717667961967	16.75945286633408
12-13	14.000576673318566	50.66246440066363	18.679231978638082	16.65772694737972
14-15	15.174517215112026	49.5451649062866	18.341220533563302	16.939097345038075
16-17	15.019412110013452	49.46594791190717	19.008188792885903	16.506451185193473
18-19	15.514346010038313	48.31061654708705	20.0257226384571	16.14931480441754
20-21	14.956977045632836	48.345708442445265	20.319921261765167	16.37739325015673
22-23	15.549086470315421	49.13041332301546	20.613271310130006	14.707228896539112
24-25	15.716569095013542	49.13327466575276	20.055097777235357	15.095058461998349
26-27	17.234879802543443	49.22435841697908	19.163277926526987	14.37748385395049
28-29	16.83546708760339	50.094024112424165	19.534763395082134	13.535745404890315
30-31	17.25208198733876	49.99779523969202	19.29406618986152	13.456056583107701
32-33	17.746676693470086	50.01543332215588	18.753573766431174	13.48431621794286
34-35	17.86452047963582	50.01260888069624	18.69006840714591	13.432802232522024
36-37	18.501404849785594	49.73889462885165	18.334506233927154	13.425194287435602
38-39	18.92638435790835	49.92223762558709	18.336158716998227	12.815219299506339
40-41	19.224068560843236	50.120888950001785	18.271005244612887	12.38403724454209
42-43	19.4976553865229	50.173141155854964	18.501668660663686	11.827534796958455
44-45	19.584684836416976	49.95508724177175	18.520374663203235	11.939853258608036
46-47	19.52583927697777	49.57390947323648	18.90822006422391	11.992031185561839
48-49	19.39565946563735	49.69317951439082	19.31152414882543	11.599636871146398
50-51	19.68272147640507	49.55151060995353	19.593272123179407	11.172495790461994
52-53	19.85832870946092	49.570335910460464	19.738106214786754	10.833229165291861
54-55	19.83526307500737	49.74107870497236	19.695680424002852	10.727977796017424
56-57	19.582841484025597	49.837854433188475	19.3989722858996	11.18033179688633
58-59	19.26928580629052	49.52780942379908	19.564647624898818	11.638257145011584
60-61	19.268737538157684	48.97123796682484	20.033541492567068	11.726483002450415
62-63	19.30740163788925	48.18192701642316	20.424741258080754	12.085930087606837
64-65	18.96936350629761	47.98219020729712	20.5760642653342	12.47238202107106
66-67	18.2861313707364	47.78150253956682	21.101437254187246	12.830928835509528
68-69	17.980756427331006	47.738504705199155	20.79122133922338	13.48951752824646
70-71	17.419812502283058	47.86501738602543	20.99443673278401	13.720733378907507
72-73	17.369990540149075	48.39218519381888	20.946813076959423	13.291011189072623
74-75	17.030120986479854	49.11156601245933	20.701717147496318	13.156595853564498
76-77	16.145717537738008	50.15362996299492	20.726497701067974	12.974154798199105
78-79	15.451809109848757	51.23060338566994	20.36855850281716	12.949029001664151
80-81	14.694753715967554	52.79879243624934	19.806106358576518	12.700347489206587
82-83	14.007227108593407	54.1938386279148	19.626476900888505	12.17245736260329
84-85	13.296003477585192	55.6531481021725	19.47233532883093	11.578513091411375
86-87	12.754521184422106	56.898138602058644	19.10058173754668	11.246758475972566
88-89	12.425991385044318	57.84783663077834	18.836738757737674	10.889433226439671
90-91	12.423681039943066	58.6554877657658	18.312525644044676	10.608305550246456
92-93	12.536322068499198	59.25045136157108	17.72046191227622	10.492764657653503
94-95	12.854642577032774	59.28460069456863	17.259956095176936	10.600800633221649
96-97	13.225420866426907	58.962280038172295	17.04201329046267	10.770285804938128
98-99	13.692761706277642	58.360267909521745	16.883793618727644	11.063176765472972
100	14.571073045577895	57.28183142008846	16.783876054051706	11.363219480281938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2283624.0
1	2312154.0
2	1486258.0
3	389356.5
4	141442.0
5	132764.0
6	114255.5
7	82313.0
8	54917.5
9	40542.5
10	37794.0
11	42577.5
12	52172.0
13	66752.5
14	87195.0
15	112398.0
16	141654.0
17	173001.0
18	206056.0
19	238891.5
20	269800.0
21	297534.5
22	320277.0
23	338934.5
24	351971.0
25	358162.0
26	358328.5
27	351756.5
28	338421.0
29	321524.0
30	302058.0
31	280389.5
32	259926.5
33	242422.0
34	226835.5
35	214260.0
36	206443.5
37	202813.0
38	203293.5
39	208617.5
40	220289.0
41	240995.5
42	273794.0
43	323207.5
44	393361.5
45	480860.0
46	576470.5
47	664675.0
48	725553.0
49	752463.0
50	750618.0
51	729842.5
52	699261.5
53	659495.5
54	604909.0
55	529044.0
56	433940.5
57	331575.5
58	236099.5
59	158141.5
60	102618.5
61	67223.0
62	46020.5
63	33874.5
64	26621.0
65	21930.5
66	18589.5
67	16006.0
68	13951.0
69	12319.0
70	10912.5
71	9684.0
72	8854.0
73	8154.0
74	7527.0
75	7020.5
76	6589.0
77	6156.5
78	5725.0
79	5310.0
80	4886.0
81	4560.0
82	4307.0
83	4070.5
84	3886.5
85	3727.5
86	3511.5
87	3417.5
88	3319.0
89	3180.0
90	3120.5
91	3038.5
92	3004.5
93	3002.0
94	2890.0
95	2738.0
96	2566.5
97	2365.0
98	2148.5
99	1877.5
100	1593.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.131394799644472E-4
2	5.609745162323195E-4
3	1.9134014507148878E-4
4	3.044047762500958E-5
5	3.087534159108115E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.696343711608307E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.261479745536741E-5
22-23	8.69727932143131E-6
24-25	1.4133078897325877E-4
26-27	2.4569814083043453E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.3917518133936097E-5
42-43	3.913775694644089E-5
44-45	1.739455864286262E-5
46-47	2.413495011697188E-4
48-49	1.3045918982146963E-5
50-51	1.0871599151789138E-5
52-53	2.1743198303578276E-6
54-55	1.0871599151789138E-5
56-57	8.69727932143131E-6
58-59	1.3045918982146963E-5
60-61	0.0
62-63	1.3045918982146963E-5
64-65	1.4567942863397444E-4
66-67	1.8699150541077317E-4
68-69	3.087534159108115E-4
70-71	9.436548063752971E-4
72-73	3.979005289554824E-4
74-75	0.0010219303202681788
76-77	0.0015024550027772587
78-79	8.218928958752587E-4
80-81	0.0013328580560093483
82-83	0.0014437483673575974
84-85	0.0018699150541077317
86-87	0.0023199992589918016
88-89	0.0024787246066079235
90-91	0.0025743946791436678
92-93	0.0023982747728846835
94-95	0.0011893529472057317
96-97	0.00211126455527745
98-99	0.0020482092801970735
100	0.0016655289900540957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2995697E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.05282487708837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.54780109443419	67.17888353676618
2	4.716261381205027	6.702073879866714
3	0.4546069425961757	0.9690332244058393
4	0.0925306781263156	0.26298264274669336
5	0.04172973355019553	0.14825077250548013
6	0.027531750550846768	0.11737251902693982
7	0.01956356907021566	0.09730327929817992
8	0.015366921876661938	0.08734905672018871
9	0.009255522002834124	0.059186788561207
>10	0.06454376103974531	0.8723709433337319
>50	0.005231970304831271	0.25440955409527877
>100	0.0037433866947151123	0.5430347424543249
>500	4.8708142706696604E-4	0.24348354389780577
>1k	7.535239371547345E-4	1.1766687208360427
>5k	1.3582322968367836E-4	0.6625504412790867
>10k+	4.5685995439053107E-4	20.625046354206383
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	972954	4.23102635245194	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	286408	1.2454851879462492	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	237477	1.0327019007077716	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226056	0.9830360871427379	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	168614	0.7332415277519094	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	140229	0.6098053909824955	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	131468	0.5717069589149657	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124909	0.5431842313803318	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	117276	0.5099910648500892	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	112451	0.48900887848713603	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107559	0.46773533326691513	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98083	0.42652762384197357	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97976	0.426062319398277	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92094	0.4004836209139475	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90470	0.3934214301049453	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84059	0.36554230124009723	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78657	0.34205094979291123	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73974	0.32168627026177987	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69647	0.3028697064498632	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66790	0.29044564293919856	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59425	0.2584179118380278	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59397	0.25829614992752775	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58422	0.25405622625832996	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50095	0.2178451038035507	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44037	0.1915010447389353	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43631	0.18973549703668474	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43435	0.18888316366318444	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41673	0.18122086058100348	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41394	0.1800075901156638	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39301	0.17090588730578596	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38551	0.16764440756024923	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32653	0.14199613084134827	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32608	0.14180044205661607	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31312	0.13616460505632857	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30596	0.13305097905925617	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29009	0.12614968791770043	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28703	0.12481900418152145	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27530	0.11971804985950198	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26352	0.11459535233917895	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25271	0.1098944728659453	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24095	0.1047804726249437	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23773	0.10338021065419326	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23676	0.10295839260710384	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23633	0.10277140110169307	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23268	0.10118414762553185	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0027265970672687156	0.0	0.0	0.0	0.0
2	0.006749088753430697	0.0	0.0	2.1743198303578275E-5	0.0
3	0.011058590657199911	0.0	0.0	4.348639660715655E-5	0.0
4	0.018020762754005673	0.0	0.0	4.78350362678722E-5	0.0
5	0.028609700327848292	0.0	0.0	7.827551389288179E-5	0.0
6	0.048239459756318756	0.0	0.0	8.69727932143131E-5	0.0
7	0.07138726867030819	0.0	0.0	8.69727932143131E-5	0.0
8	0.1500585087723151	0.0	0.0	8.69727932143131E-5	0.0
9	0.43555105113795856	0.0	0.0	8.69727932143131E-5	0.0
10-11	1.0559301594554842	0.0	0.0	9.349575270538657E-5	0.0
12-13	1.8730765151410718	0.0	0.0	9.784439236610224E-5	0.0
14-15	2.8642402098096875	0.0	0.0	1.0001871219646005E-4	0.0
16-17	3.9759568931526625	0.0	0.0	1.1306463117860703E-4	0.0
18-19	5.224236082080921	0.0	0.0	1.2176191050003834E-4	0.0
20-21	6.88449452086623	0.0	0.0	1.2176191050003834E-4	0.0
22-23	8.741794171318226	0.0	0.0	1.2176191050003834E-4	0.0
24-25	10.630730175301927	0.0	0.0	1.2176191050003834E-4	0.0
26-27	12.503217449768972	0.0	0.0	1.2176191050003834E-4	0.0
28-29	14.512095458554702	0.0	0.0	1.2176191050003834E-4	0.0
30-31	16.668892445399678	2.174319830357827E-6	0.0	1.2176191050003834E-4	0.0
32-33	19.044373823502717	4.348639660715654E-6	0.0	1.2393623033039617E-4	0.0
34-35	21.178331754849616	6.5229594910734816E-6	0.0	1.26110550160754E-4	0.0
36-37	22.85503457451192	8.697279321431309E-6	0.0	1.3698214931254313E-4	0.0
38-39	24.105544615586126	8.697279321431309E-6	0.0	1.6089966744647924E-4	0.0
40-41	25.146469793892308	8.697279321431309E-6	0.0	1.6089966744647924E-4	0.0
42-43	25.971195828506524	8.697279321431309E-6	0.0	1.739455864286262E-4	0.0
44-45	26.6259074469454	8.697279321431309E-6	0.0	1.8699150541077314E-4	0.0
46-47	27.176825733962318	8.697279321431309E-6	0.0	2.000374243929201E-4	0.0
48-49	27.680328628438616	8.697279321431309E-6	0.0	2.1090902354470927E-4	0.0
50-51	28.185203518727874	8.697279321431309E-6	0.0	2.543954201518658E-4	0.0
52-53	28.71656814751038	8.697279321431309E-6	0.0	2.913588572679488E-4	0.0
54-55	29.262205011659354	8.697279321431309E-6	0.0	3.5658845217868373E-4	0.0
56-57	29.834549046284614	8.697279321431309E-6	0.0	3.870289298036933E-4	0.0
58-59	30.42992347655303	8.697279321431309E-6	0.0	4.0877212810727154E-4	0.0
60-61	31.03648913098829	8.697279321431309E-6	0.0	4.2181804708941854E-4	0.0
62-63	31.60756336283262	8.697279321431309E-6	0.0	4.500842048840703E-4	0.0
64-65	32.142130764725245	8.697279321431309E-6	0.0	4.805246825090798E-4	0.0
66-67	32.62253368532382	1.9568878473220446E-5	0.0	5.153137997948051E-4	0.0
68-69	33.036806842601905	2.609183796429393E-5	0.0	5.348826782680255E-4	0.0
70-71	33.405138796184346	2.609183796429393E-5	0.0	5.566258765716038E-4	0.0
72-73	33.74488931559674	2.609183796429393E-5	0.0	5.718461153841086E-4	0.0
74-75	34.06837592267806	2.609183796429393E-5	0.0	5.935893136876869E-4	0.0
76-77	34.380486488406945	2.826615779465176E-5	0.0	6.349013904644856E-4	0.0
78-79	34.68262997203347	3.4789117285725235E-5	0.0	6.870850663930735E-4	0.0
80-81	34.97429975703715	3.4789117285725235E-5	0.0	7.370944224913034E-4	0.0
82-83	35.26334948664526	3.4789117285725235E-5	4.348639660715654E-6	7.827551389288179E-4	0.0
84-85	35.553160228194	3.4789117285725235E-5	4.348639660715654E-6	8.023240174020382E-4	0.0
86-87	35.84241651818599	3.696343711608307E-5	8.697279321431309E-6	8.088469768931118E-4	0.0
88	36.06046383373376	3.913775694644089E-5	8.697279321431309E-6	8.175442562145431E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	8350	0.0	45.08572	9
GCTTGCT	2120	0.0	42.122166	7
GCCTGCT	2120	0.0	38.79673	7
CTTGCTG	5905	0.0	38.76163	8
CTGCTGA	8790	0.0	38.177044	9
CCTGCTG	6960	0.0	37.410496	8
TACCCGA	15430	0.0	35.577045	6
ACCCGAC	16420	0.0	33.975864	7
TGAAAAA	20640	0.0	33.22363	1
CTACCCG	17020	0.0	31.535553	5
CCCGACG	18605	0.0	31.349821	8
TGACGCC	1275	0.0	29.858616	3
TGCTTGC	1340	0.0	29.462355	6
TTGCGCC	1325	0.0	29.086592	3
GACACGC	1410	0.0	29.000114	2
TGCCAAA	2735	0.0	28.182499	6
GCTGAAC	16880	0.0	28.135645	10-11
CCTCACG	4380	0.0	28.006657	4
ACTTGCC	1450	0.0	27.875668	3
GTTGCTG	2840	0.0	27.802504	8
>>END_MODULE