##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248459_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19654356
Sequences flagged as poor quality	0
Sequence length	100
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.838550344768354	31.0	28.0	33.0	19.0	34.0
2	29.371193897169665	31.0	29.0	33.0	23.0	34.0
3	29.717993914427925	31.0	30.0	34.0	25.0	34.0
4	33.10467704970847	35.0	33.0	37.0	27.0	37.0
5	32.86567262748268	35.0	32.0	37.0	26.0	37.0
6	32.73220145193259	35.0	32.0	37.0	25.0	37.0
7	32.859388269959084	35.0	32.0	37.0	26.0	37.0
8	32.732708057185896	35.0	32.0	37.0	25.0	37.0
9	33.96069527793228	37.0	32.0	39.0	25.0	39.0
10-11	34.265969564202464	37.0	33.0	39.0	27.0	39.0
12-13	34.13412617030036	37.0	32.5	39.0	27.0	39.0
14-15	34.84681914787745	37.0	32.0	40.0	27.0	41.0
16-17	34.626631368639096	37.0	32.0	40.0	26.0	41.0
18-19	34.138129252365225	36.5	32.0	39.5	25.0	41.0
20-21	33.800015630122914	35.5	32.0	39.0	25.0	40.5
22-23	33.27644675816394	35.0	31.5	38.5	25.0	40.0
24-25	32.77037108211533	35.0	30.5	38.0	23.5	40.0
26-27	32.29143152795238	35.0	30.0	38.0	22.0	40.0
28-29	32.07799461859753	35.0	30.0	37.0	22.0	40.0
30-31	31.98705874667173	35.0	30.0	37.0	22.0	40.0
32-33	31.789078054757937	35.0	30.0	37.0	21.5	40.0
34-35	31.607211195319756	34.5	28.5	37.0	21.0	40.0
36-37	31.42276434801527	34.0	28.0	37.0	20.5	40.0
38-39	31.21453188290677	34.0	27.5	37.0	20.0	39.5
40-41	30.959119113340577	33.5	26.5	37.0	19.5	39.0
42-43	30.68880845040153	33.0	26.0	36.5	18.0	39.0
44-45	30.451537893177473	33.0	26.0	36.0	18.5	39.0
46-47	30.301867255279184	33.0	25.5	35.0	18.5	39.0
48-49	30.075142502761217	33.0	25.5	35.0	18.0	39.0
50-51	29.845207113374766	33.0	26.0	35.0	18.0	38.5
52-53	29.543508497556473	33.0	26.0	35.0	17.0	38.0
54-55	29.308236199649585	32.0	26.0	35.0	15.5	37.0
56-57	29.06804850283571	32.0	26.0	35.0	15.0	37.0
58-59	28.75996094707962	31.0	26.0	35.0	14.5	36.0
60-61	28.481728859495576	31.0	25.5	35.0	14.0	35.5
62-63	28.426702024731817	31.0	25.5	35.0	14.0	35.0
64-65	28.682386388035304	31.0	26.0	35.0	16.5	35.0
66-67	29.15466123642006	32.0	26.0	35.0	18.5	35.0
68-69	29.19907515667265	32.0	26.0	35.0	18.5	35.0
70-71	29.527091984087395	32.5	27.0	35.0	20.0	35.0
72-73	29.56732784834059	33.0	27.0	35.0	20.0	35.0
74-75	29.513137291295628	33.0	27.0	35.0	19.0	35.0
76-77	29.423743494826287	33.0	27.0	35.0	19.0	35.0
78-79	29.318673249838355	33.0	27.0	35.0	18.5	35.0
80-81	29.238499063515487	33.0	27.0	35.0	18.0	35.0
82-83	29.1524414740427	33.0	27.0	35.0	17.5	35.0
84-85	29.06840203769587	33.0	27.0	35.0	17.0	35.0
86-87	28.91664257531511	33.0	27.0	35.0	15.0	35.0
88-89	28.77208864029938	33.0	26.5	35.0	15.0	35.0
90-91	28.564282136743632	33.0	26.0	35.0	10.5	35.0
92-93	28.345942700946296	33.0	25.5	35.0	10.0	35.0
94-95	28.04653538381008	32.5	25.0	35.0	10.0	35.0
96-97	27.700343781297132	32.0	25.0	35.0	8.0	35.0
98-99	27.275177243151596	32.0	24.0	35.0	7.5	35.0
100	25.94015581075259	30.0	20.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6790260794368734
1101	2	0.6276121095646516
1101	3	0.5727671152681957
1101	4	0.5551428146344506
1101	5	0.42538962528310265
1101	6	0.26851253569983413
1101	7	0.3934132272938342
1101	8	0.5048777249468586
1101	9	0.45792567669357
1101	10-11	0.5040258186057542
1101	12-13	0.5917118932825858
1101	14-15	0.7448910066267729
1101	16-17	0.7230636428296293
1101	18-19	0.7938552157540002
1101	20-21	0.7009823669261266
1101	22-23	0.6353054462614409
1101	24-25	0.5672694815426524
1101	26-27	0.671813003155151
1101	28-29	0.5245500315748046
1101	30-31	0.5620001430875732
1101	32-33	0.5239747304802727
1101	34-35	0.5170857270348463
1101	36-37	0.5030614384919154
1101	38-39	0.4492949708014642
1101	40-41	0.47499005059543364
1101	42-43	0.39600550803192647
1101	44-45	0.45274750954845544
1101	46-47	0.4217793412555437
1101	48-49	0.3854382946389805
1101	50-51	0.3967059521851084
1101	52-53	0.3643254362863111
1101	54-55	0.32545153494136514
1101	56-57	0.2896913629196405
1101	58-59	0.2697275555369245
1101	60-61	0.2127772664772145
1101	62-63	0.31852062746888166
1101	64-65	0.40537710852334996
1101	66-67	0.46427313897674694
1101	68-69	0.4973914869920648
1101	70-71	0.46933007892040024
1101	72-73	0.48679488705662166
1101	74-75	0.5186159765796816
1101	76-77	0.5445755233336982
1101	78-79	0.48597132779342544
1101	80-81	0.4920057757036318
1101	82-83	0.4246621654927445
1101	84-85	0.40193686852182964
1101	86-87	0.3451475703765432
1101	88-89	0.36210057474664126
1101	90-91	0.4026111227195699
1101	92-93	0.30839650945789643
1101	94-95	0.3475681714833101
1101	96-97	0.3351133289125734
1101	98-99	0.3145792264406282
1101	100	0.20780407899989584
1102	1	0.42852719571254383
1102	2	0.4411693688269871
1102	3	0.39622036309066644
1102	4	0.44259475114219526
1102	5	0.38093450501965975
1102	6	0.33859285032368547
1102	7	0.33978044994881884
1102	8	0.39886752870779674
1102	9	0.3792643693043303
1102	10-11	0.399805403701194
1102	12-13	0.428183692554164
1102	14-15	0.5660607884781967
1102	16-17	0.6128803596376997
1102	18-19	0.6932491053072383
1102	20-21	0.5496696685616911
1102	22-23	0.4401160589644846
1102	24-25	0.40510131912908065
1102	26-27	0.5228826730534379
1102	28-29	0.3515408923107657
1102	30-31	0.3407460558042956
1102	32-33	0.32968696659471775
1102	34-35	0.31519818248847287
1102	36-37	0.29896624179324505
1102	38-39	0.2713279255759069
1102	40-41	0.3226149283317845
1102	42-43	0.3298855151743325
1102	44-45	0.29307551357210926
1102	46-47	0.27474059483765956
1102	48-49	0.36502786786482844
1102	50-51	0.3480342710104516
1102	52-53	0.26311006576149865
1102	54-55	0.2432099325967485
1102	56-57	0.2406147768318334
1102	58-59	0.25192666941922326
1102	60-61	0.27222137319002115
1102	62-63	0.2810169388217183
1102	64-65	0.35156312120764355
1102	66-67	0.3774997093496353
1102	68-69	0.4079362137920235
1102	70-71	0.42772548770389207
1102	72-73	0.37708366241945157
1102	74-75	0.3617932945057589
1102	76-77	0.40407302979389215
1102	78-79	0.3585103476435485
1102	80-81	0.32507245426374
1102	82-83	0.2803265971239668
1102	84-85	0.24447186876698979
1102	86-87	0.2571412933777637
1102	88-89	0.26169961429001987
1102	90-91	0.272554297195466
1102	92-93	0.25698234383606433
1102	94-95	0.2321768079995259
1102	96-97	0.12548536036343805
1102	98-99	0.18413487307893917
1102	100	0.16669685857644367
1103	1	0.2521281769316488
1103	2	0.25440830230940037
1103	3	0.29169239503251987
1103	4	0.2670421780866121
1103	5	0.27101227483246504
1103	6	0.13985070287771606
1103	7	0.2510849113458278
1103	8	0.2910146898560271
1103	9	0.32304026551908294
1103	10-11	0.2850603260420783
1103	12-13	0.33034881555596485
1103	14-15	0.44205706703769465
1103	16-17	0.4082481221921057
1103	18-19	0.473956738294639
1103	20-21	0.41049683534196646
1103	22-23	0.3037009217047313
1103	24-25	0.3303712043007323
1103	26-27	0.405098309001076
1103	28-29	0.19282646414713867
1103	30-31	0.1377550080692984
1103	32-33	0.1847528625117505
1103	34-35	0.2172216744305544
1103	36-37	0.13748848146432735
1103	38-39	0.05258231293885629
1103	40-41	0.0800302744489052
1103	42-43	0.05658538521274892
1103	44-45	0.12673142686444194
1103	46-47	0.16276411984639338
1103	48-49	0.14288857342105743
1103	50-51	0.10150123547092349
1103	52-53	0.09803760079581281
1103	54-55	0.025922528175954085
1103	56-57	0.08768278440694388
1103	58-59	0.07699786883424409
1103	60-61	0.09035735208225404
1103	62-63	0.12439041381197313
1103	64-65	0.1941355202132904
1103	66-67	0.13098281061519046
1103	68-69	0.1797012763243977
1103	70-71	0.1748645495429031
1103	72-73	0.12141580566700583
1103	74-75	0.18784643251525424
1103	76-77	0.15754691932218634
1103	78-79	0.20843540179560094
1103	80-81	0.21370643030130054
1103	82-83	0.1841123674773506
1103	84-85	0.179909968944866
1103	86-87	0.13351883760005023
1103	88-89	0.1334004106589859
1103	90-91	0.1408199811972004
1103	92-93	0.13980000184351482
1103	94-95	0.18693830253451793
1103	96-97	0.18106973430018058
1103	98-99	0.21051649420206076
1103	100	0.15229071367887315
1104	1	0.23379272842677778
1104	2	0.2746221248611498
1104	3	0.2337498103857918
1104	4	0.2401919158031518
1104	5	0.23023196482960628
1104	6	0.1490863313022004
1104	7	0.203090486212254
1104	8	0.32218280333325566
1104	9	0.19428420064706842
1104	10-11	0.24096435732327848
1104	12-13	0.33663452486009504
1104	14-15	0.3977369030840805
1104	16-17	0.4367134377046895
1104	18-19	0.4835013410709834
1104	20-21	0.38151771893891606
1104	22-23	0.3055316559005661
1104	24-25	0.32433682727378965
1104	26-27	0.34319829474094377
1104	28-29	0.1367289767442088
1104	30-31	0.15187893328180024
1104	32-33	0.181040239816614
1104	34-35	0.17858068932136106
1104	36-37	0.15000619109986957
1104	38-39	0.1089344779266419
1104	40-41	0.15810060121732405
1104	42-43	0.11162137253807103
1104	44-45	0.11533537516658754
1104	46-47	0.08437981307784526
1104	48-49	0.17200029349049473
1104	50-51	0.11865184949475704
1104	52-53	0.08681857043993801
1104	54-55	0.055316040838437885
1104	56-57	0.0582964704421407
1104	58-59	0.05348505966743389
1104	60-61	0.08739963998930378
1104	62-63	0.12433220856948068
1104	64-65	0.1986250481631764
1104	66-67	0.2380416999711592
1104	68-69	0.23897420596614083
1104	70-71	0.27054496205539635
1104	72-73	0.24290742997901305
1104	74-75	0.23740774091036343
1104	76-77	0.24020341467748807
1104	78-79	0.24135882645519757
1104	80-81	0.21474207974406667
1104	82-83	0.16933308794072133
1104	84-85	0.15362961047393497
1104	86-87	0.18814729620278214
1104	88-89	0.22890653601256616
1104	90-91	0.19607429683169286
1104	92-93	0.21295185406408734
1104	94-95	0.22010569875756758
1104	96-97	0.22982456238045756
1104	98-99	0.22631740231142317
1104	100	0.225989364277293
1105	1	0.23454503220288814
1105	2	0.19201578186315515
1105	3	0.18072300425134102
1105	4	0.23340628978454703
1105	5	0.17854422164484873
1105	6	0.11254484506801532
1105	7	0.0457580337939234
1105	8	0.1745253121525181
1105	9	0.1668700646981307
1105	10-11	0.19183884909000426
1105	12-13	0.21535314773406355
1105	14-15	0.3269351073653439
1105	16-17	0.3034553216987419
1105	18-19	0.34901632697695817
1105	20-21	0.27509417641652334
1105	22-23	0.16218157955581347
1105	24-25	0.0810984011684539
1105	26-27	0.1959592418799474
1105	28-29	0.05456229961581016
1105	30-31	0.04802937707172816
1105	32-33	0.03234415117734457
1105	34-35	0.02017966639525781
1105	36-37	-0.0608762344666971
1105	38-39	-0.050915939623092044
1105	40-41	0.01886058579121297
1105	42-43	-0.06726406425776332
1105	44-45	-0.07866362714349506
1105	46-47	-0.09802619599559392
1105	48-49	-0.052879711049506284
1105	50-51	-0.09040303386018422
1105	52-53	-0.04806915460254757
1105	54-55	-0.16319770059903504
1105	56-57	-0.12245266754568007
1105	58-59	-0.06493581216276212
1105	60-61	-0.0734841358449394
1105	62-63	-0.0315652838852607
1105	64-65	0.0365370680946171
1105	66-67	0.027480545775521392
1105	68-69	0.0120062480736145
1105	70-71	0.02359797083128612
1105	72-73	0.018514021651562018
1105	74-75	0.06027835508505319
1105	76-77	0.023672676290857453
1105	78-79	0.008523174641158704
1105	80-81	0.01593100105353784
1105	82-83	-0.010184210108590719
1105	84-85	-0.036737328137935066
1105	86-87	-0.011076418380703501
1105	88-89	0.02124023506622308
1105	90-91	-9.661318692799625E-4
1105	92-93	-0.0016174732430194183
1105	94-95	0.025785871985309683
1105	96-97	0.05633471212437158
1105	98-99	0.057816492264244346
1105	100	0.07544282966095395
1106	1	0.039598735717149225
1106	2	0.09121832783384676
1106	3	0.14744980720324108
1106	4	0.13906183567851826
1106	5	0.12062297993178817
1106	6	0.11710184702022275
1106	7	0.10138201968231897
1106	8	0.22916584066983603
1106	9	0.1655841853941027
1106	10-11	0.17775658634593583
1106	12-13	0.24389853340194634
1106	14-15	0.31650537801769474
1106	16-17	0.23260214126004541
1106	18-19	0.31895791699027853
1106	20-21	0.21570194895146955
1106	22-23	0.18542062182241636
1106	24-25	0.16341527854300608
1106	26-27	0.2266064138050723
1106	28-29	0.020167425870042166
1106	30-31	0.07324326404250314
1106	32-33	0.0496983624223013
1106	34-35	0.027397512486150077
1106	36-37	0.020742522086845128
1106	38-39	-0.029455562082308262
1106	40-41	0.026967049394020393
1106	42-43	0.0028341713656487855
1106	44-45	-0.013749482080424258
1106	46-47	-0.010710219066901061
1106	48-49	-0.01809508810721283
1106	50-51	-0.019814534773772152
1106	52-53	-0.06823182449732101
1106	54-55	-0.05658216753509038
1106	56-57	-0.049667311459302255
1106	58-59	-0.0707878399597206
1106	60-61	-0.017042182811625395
1106	62-63	0.010135580187554893
1106	64-65	0.07264230055528031
1106	66-67	0.052426163818818594
1106	68-69	0.050728066072014855
1106	70-71	0.06919746352684086
1106	72-73	0.13368841687873356
1106	74-75	0.11932277423886717
1106	76-77	0.16207893863424516
1106	78-79	0.1607180868912046
1106	80-81	0.11812422607761874
1106	82-83	0.10023574220863196
1106	84-85	0.1423768125205882
1106	86-87	0.16491440950377623
1106	88-89	0.14722754641660174
1106	90-91	0.15226014608894545
1106	92-93	0.13790785503787006
1106	94-95	0.1437369793738661
1106	96-97	0.15423344091505342
1106	98-99	0.15774910978752388
1106	100	0.14705484441476813
1107	1	0.025290905360058957
1107	2	0.07684711423549118
1107	3	0.08128266739988632
1107	4	0.10186849324598057
1107	5	0.05416697682417748
1107	6	0.015708407151272752
1107	7	0.025469968163847057
1107	8	0.17210774221113923
1107	9	0.16537710547365947
1107	10-11	0.1078352046986879
1107	12-13	0.14473422863197527
1107	14-15	0.19560274551325563
1107	16-17	0.21407280354951297
1107	18-19	0.20273664049063456
1107	20-21	0.11106782743795662
1107	22-23	0.08514989875224899
1107	24-25	0.08356487015278447
1107	26-27	0.19161094860540118
1107	28-29	9.607873464716477E-4
1107	30-31	0.02576147415925334
1107	32-33	0.004580491694365918
1107	34-35	-0.0021041710322222684
1107	36-37	-0.07104253891143486
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2216	38-39	0.8853300397945425
2216	40-41	0.9290535645340974
2216	42-43	0.9006403458612553
2216	44-45	0.9270789599811771
2216	46-47	0.815625722380112
2216	48-49	0.8588940673759495
2216	50-51	0.7997664914472189
2216	52-53	0.7985657922689384
2216	54-55	0.7884545950358302
2216	56-57	0.8089030301820515
2216	58-59	0.801501611540349
2216	60-61	0.7512876251282243
2216	62-63	0.6247293118588964
2216	64-65	0.5352686170731893
2216	66-67	0.5549886191767435
2216	68-69	0.5540623454786875
2216	70-71	0.4875573655513925
2216	72-73	0.4818915429373192
2216	74-75	0.48866039224397184
2216	76-77	0.4888365602107214
2216	78-79	0.41364709727429627
2216	80-81	0.4323237403621185
2216	82-83	0.3896045395001977
2216	84-85	0.32897399602881094
2216	86-87	0.29115213232703496
2216	88-89	0.21614060493861942
2216	90-91	0.1808171277714159
2216	92-93	0.14269674034571622
2216	94-95	0.06877263254676791
2216	96-97	0.04326843885353426
2216	98-99	0.06098165606527317
2216	100	0.24963394679200235
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	490.0
6	4835.0
7	14827.0
8	25568.0
9	41310.0
10	60968.0
11	77412.0
12	84757.0
13	89430.0
14	96048.0
15	107462.0
16	120716.0
17	139963.0
18	163772.0
19	189556.0
20	215783.0
21	244106.0
22	273059.0
23	316485.0
24	417470.0
25	477161.0
26	548705.0
27	854913.0
28	786349.0
29	1242041.0
30	1237183.0
31	1510808.0
32	1917447.0
33	2781692.0
34	2101691.0
35	3232088.0
36	230851.0
37	43238.0
38	5522.0
39	648.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.091229544433833	32.16942373828924	25.393066445130152	26.346280272146778
2	13.67346546276167	35.05374711563862	27.02066584635273	24.252121575246978
3	12.00465220830309	37.90912757000725	24.24446897735775	25.841751244331906
4	10.919460021854704	37.768405581033946	23.937890393836966	27.374244003274388
5	10.805993353314026	37.26826070582192	24.91580216569336	27.00994377517069
6	10.549223795478213	34.10706003290059	26.339504586158917	29.004211585462276
7	14.627871806127862	38.519893503506296	21.86247160680309	24.98976308356275
8	12.661793650221863	36.147452503658734	19.796507196674366	31.39424664944504
9	10.364384363445946	35.140886834450335	20.595424240814605	33.899304561289114
10-11	15.007156683230935	48.10774517363988	19.45115372897489	17.433944414154297
12-13	14.644005016701136	49.373904504600766	18.560194053733653	17.421896424964448
14-15	16.09413709612261	48.33037521046225	17.950183664120058	17.625304029295084
16-17	16.08443695636733	48.2202266001491	18.53294506317073	17.16239138031284
18-19	16.708901578866282	46.63960752517152	19.622942618928853	17.028548277033345
20-21	15.714154481980975	46.50043510722982	20.462782527461286	17.322627883327918
22-23	16.11892631429523	47.29461740158861	21.01070729616922	15.575748987946946
24-25	16.23205688556459	47.40341011774704	20.307251794209684	16.05728120247868
26-27	17.96440334926859	47.603878047987514	19.119500490502826	15.31221811224107
28-29	17.362232575821867	48.63612168213499	19.790078596317272	14.211567145725864
30-31	17.684023836751507	48.41057880502419	19.597291816633422	14.308105541590882
32-33	18.23218221955479	48.61458192779249	18.94581282642891	14.207423026223806
34-35	18.34954042757748	48.671869482775215	18.757050599877196	14.221539489770105
36-37	18.998722217100372	48.39303562019534	18.3633388954591	14.244903267245185
38-39	19.4239307561133	48.552783922302005	18.260094607017397	13.763190714567294
40-41	19.771578851474306	48.70870466433811	18.204434961445056	13.31528152274253
42-43	19.98634398827028	48.82971566468945	18.43152768108939	12.75241266595088
44-45	20.157116331577214	48.44443628114157	18.48387385309128	12.91457353418993
46-47	20.218492545324285	47.95264234423098	18.861416089469234	12.967449020975502
48-49	20.115811483002112	48.078181654905485	19.239641799489224	12.566365062603174
50-51	20.39177067881393	47.98172974895385	19.493150500232645	12.133349071999564
52-53	20.5610481605464	48.09127167766961	19.600566398628537	11.747113763155449
54-55	20.532223533466844	48.32761869640644	19.566376499416453	11.573781270710263
56-57	20.371597032820464	48.48758201426605	19.1495498071547	11.991271145758791
58-59	20.078313432315635	48.343563042389334	19.310048587196068	12.26807493809896
60-61	20.020633085103373	47.9098831831478	19.74068496571447	12.328798766034359
62-63	20.059573059485956	47.23133612023066	20.09290409646636	12.616186723817021
64-65	19.722540620997307	47.09577196869505	20.252189637640527	12.929497772667117
66-67	19.11633418396012	46.93034443477991	20.693342874233238	13.259978507026734
68-69	18.892498588921693	46.85251374184335	20.430017448348632	13.824970220886321
70-71	18.451201571492838	46.86470988509415	20.641036221407987	14.04305232200502
72-73	18.482045254785536	47.24490982241777	20.576589571479996	13.696455351316697
74-75	18.283748407558488	47.785298854179864	20.359047877638012	13.571904860623638
76-77	17.567452812452665	48.58593686916702	20.39098969460427	13.45562062377605
78-79	16.939931845100077	49.456132772031744	20.112823725026296	13.491111657841879
80-81	16.290799834172205	50.7357005583154	19.654821552972802	13.318678054539596
82-83	15.654079079284713	51.930541450858335	19.57006652628721	12.845312943569741
84-85	14.991233341185845	53.25100831840424	19.48708940312944	12.270668937280483
86-87	14.433748071821432	54.43985169160479	19.17929210120062	11.947108135373158
88-89	14.068785049486978	55.37882988408285	18.923771265332352	11.628613801097822
90-91	13.983494597182972	56.217144030303444	18.46519012097746	11.334171251536116
92-93	14.01184003560004	56.90480273633077	17.912071493354976	11.17128573471422
94-95	14.216759714911987	57.07146142891719	17.48078941735485	11.230989438815971
96-97	14.490611265565475	56.88297268028033	17.28388133206889	11.342534722085304
98-99	14.8595772325094	56.42801141815056	17.10862400275242	11.603787346587618
100	15.617213588398226	55.51831581012567	16.998202557022527	11.866268044453578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2006971.0
1	2001149.5
2	1261818.0
3	332851.0
4	132185.0
5	121919.0
6	102298.0
7	72280.5
8	46785.0
9	33377.0
10	30360.5
11	33207.5
12	39827.0
13	50083.5
14	64200.0
15	82145.5
16	102699.0
17	124830.5
18	147745.0
19	170595.5
20	192476.5
21	211533.5
22	227051.0
23	239551.5
24	248649.5
25	253696.0
26	253876.5
27	248635.5
28	239305.0
29	226930.5
30	212154.5
31	195936.0
32	179829.0
33	165855.0
34	154115.5
35	144520.0
36	137857.0
37	134761.5
38	134970.0
39	138810.0
40	148333.0
41	164880.0
42	190948.0
43	230498.5
44	287366.0
45	360109.0
46	440830.0
47	518912.5
48	585019.5
49	635962.5
50	674932.5
51	706952.0
52	731442.0
53	737005.0
54	709828.0
55	640832.0
56	534015.0
57	409236.0
58	289805.0
59	192696.0
60	123506.5
61	79642.0
62	53868.0
63	38846.5
64	29955.5
65	24368.0
66	20515.5
67	17732.5
68	15409.5
69	13351.0
70	11654.0
71	10263.0
72	9105.0
73	8212.0
74	7574.0
75	7019.0
76	6625.5
77	6236.0
78	5793.5
79	5367.5
80	5001.5
81	4705.5
82	4440.0
83	4295.5
84	4179.0
85	4007.0
86	3936.0
87	3813.5
88	3677.0
89	3607.5
90	3532.0
91	3501.0
92	3462.0
93	3388.5
94	3260.5
95	3123.0
96	2988.5
97	2808.5
98	2577.0
99	2212.0
100	1911.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.545844391950568E-4
2	4.6808961840316725E-4
3	1.7298964158377918E-4
4	2.5439653174085177E-5
5	2.5439653174085176E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.052758380890221E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.798361849149369E-5
22-23	5.087930634817035E-6
24-25	1.0175861269634071E-4
26-27	1.7807757221859622E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.2719826587042589E-5
42-43	4.3247410395944795E-5
44-45	1.017586126963407E-5
46-47	2.3913273983640064E-4
48-49	5.087930634817035E-6
50-51	5.087930634817035E-6
52-53	2.5439653174085176E-6
54-55	7.631895952225552E-6
56-57	5.087930634817035E-6
58-59	5.087930634817035E-6
60-61	0.0
62-63	1.017586126963407E-5
64-65	9.921464737893218E-5
66-67	1.2974223118783438E-4
68-69	3.078198034064306E-4
70-71	9.056516529974321E-4
72-73	4.1212238142017985E-4
74-75	9.183714795844749E-4
76-77	0.0014398843696532208
78-79	7.962611443488659E-4
80-81	0.001180399907277552
82-83	0.001249086970847582
84-85	0.001760423999646694
86-87	0.0022641291324935805
88-89	0.0022819368897154402
90-91	0.0024854541151081216
92-93	0.0022666730978109888
94-95	0.0010939050864856624
96-97	0.0020478920805138564
98-99	0.0019588532944045584
100	0.0016535774563155364
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9654356E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.2587409621703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.12686124015897	67.78620363656806
2	4.20957895070713	5.9993859201648805
3	0.3799313778935596	0.812202949221528
4	0.08511535216129959	0.24260851326263796
5	0.04226171754267672	0.15057583914950046
6	0.025815029118726556	0.11037278837413315
7	0.01826450904170532	0.09110541430228677
8	0.016311043584144886	0.09298435436682001
9	0.012697683435903594	0.08143388413008253
>10	0.07110569475888898	0.9487466667694502
>50	0.005436059450488516	0.2747983687435296
>100	0.00449384163373505	0.6282675115498727
>500	6.134795430597751E-4	0.31256890125723796
>1k	8.796264792804612E-4	1.3614018104841195
>5k	1.5859862262129233E-4	0.8362573166174161
>10k+	4.75795867863877E-4	20.271086125038476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	862367	4.387663477755262	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	249309	1.2684668986356002	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	205941	1.0478135228648549	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	199226	1.0136480686520586	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	144377	0.7345801612629791	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118966	0.6052907559016434	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113938	0.5797086406697833	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105036	0.5344158821586421	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98581	0.5015732899108981	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95208	0.48441169987966026	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86659	0.4409149808826094	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81844	0.41641659487596544	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81801	0.41619781385866833	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80217	0.4081385317331181	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75503	0.38415402672059057	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71995	0.36630556605365244	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66367	0.3376706924409022	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59463	0.30254361933812535	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58139	0.2958071991776276	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53135	0.2703471942810032	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52708	0.2681746478999363	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48060	0.24452594630930669	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47728	0.24283675333854746	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41193	0.2095871266400181	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41137	0.20930220252446838	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36082	0.18358271316546826	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35960	0.18296198562802057	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35823	0.18226493913105066	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34936	0.17775194465796793	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34518	0.1756251896526144	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32961	0.16770328165420428	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31085	0.15815832378328754	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29335	0.1492544451723577	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26983	0.13728763231926805	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25511	0.12979819842481738	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23848	0.12133696977911666	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23511	0.1196223371551833	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23450	0.11931197338645946	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22694	0.1154654978265378	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22190	0.11290118078659	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19798	0.10073085070810765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002915384253750161	0.0	0.0	5.087930634817035E-6	0.0
2	0.006899233940811899	0.0	0.0	2.5439653174085174E-5	0.0
3	0.011295206009293818	0.0	0.0	3.052758380890221E-5	0.0
4	0.018204615811375353	0.0	0.0	4.5791375713353315E-5	0.0
5	0.028558554653228017	0.0	0.0	5.087930634817035E-5	0.0
6	0.047821460036645315	0.0	0.0	6.105516761780442E-5	0.0
7	0.07124120474870813	0.0	0.0	6.614309825262146E-5	0.0
8	0.15630122910157931	0.0	0.0	6.614309825262146E-5	0.0
9	0.46737222018365804	0.0	0.0	6.614309825262146E-5	0.0
10-11	1.1331432075413714	0.0	0.0	6.614309825262146E-5	0.0
12-13	1.987676421450797	0.0	0.0	7.123102888743849E-5	0.0
14-15	2.969115345219146	0.0	0.0	7.631895952225552E-5	0.0
16-17	4.004735642317662	0.0	0.0	9.158275142670663E-5	0.0
18-19	5.1440632295456545	0.0	0.0	1.0684654333115773E-4	0.0
20-21	6.618446312868252	0.0	0.0	1.0939050864856625E-4	0.0
22-23	8.280902208141544	0.0	0.0	1.1193447396597477E-4	0.0
24-25	10.024482613421675	0.0	0.0	1.1193447396597477E-4	0.0
26-27	11.818260542344913	2.5439653174085176E-6	0.0	1.1193447396597477E-4	0.0
28-29	13.790352632261266	5.087930634817035E-6	0.0	1.1193447396597477E-4	0.0
30-31	15.951809868509557	5.087930634817035E-6	0.0	1.144784392833833E-4	0.0
32-33	18.367734867527584	5.087930634817035E-6	0.0	1.170224046007918E-4	0.0
34-35	20.604409526315692	5.087930634817035E-6	0.0	1.170224046007918E-4	0.0
36-37	22.38664294062853	5.087930634817035E-6	0.0	1.170224046007918E-4	0.0
38-39	23.740671024784533	5.087930634817035E-6	0.0	1.2211033523560884E-4	0.0
40-41	24.915812555751	5.087930634817035E-6	0.0	1.3228619650524292E-4	0.0
42-43	25.875480224332968	5.087930634817035E-6	0.0	1.5518188436191956E-4	0.0
44-45	26.661746128949737	7.631895952225552E-6	0.0	1.8062153753600475E-4	0.0
46-47	27.335454288097765	1.017586126963407E-5	0.0	2.0097326007527288E-4	0.0
48-49	27.95075554752341	1.017586126963407E-5	0.0	2.315008438841751E-4	0.0
50-51	28.566260324174447	1.017586126963407E-5	0.0	2.6711635832789434E-4	0.0
52-53	29.212358827732643	1.017586126963407E-5	0.0	3.027318727716136E-4	0.0
54-55	29.87327592926474	1.017586126963407E-5	0.0	3.281715259456987E-4	0.0
56-57	30.56625971362277	1.017586126963407E-5	0.0	3.4343531785014985E-4	0.0
58-59	31.282184977213195	1.017586126963407E-5	0.0	3.663310057068265E-4	0.0
60-61	32.01043575276646	1.017586126963407E-5	0.0	4.0194652015054577E-4	0.0
62-63	32.70899336513493	1.017586126963407E-5	0.0	4.350180692768565E-4	0.0
64-65	33.370798310562805	1.017586126963407E-5	0.0	4.655456530857587E-4	0.0
66-67	33.97383765715854	1.017586126963407E-5	0.0	4.935292715772524E-4	0.0
68-69	34.50351921986149	1.017586126963407E-5	0.0	5.266008207035632E-4	0.0
70-71	34.981265730609536	1.017586126963407E-5	0.0	5.418646126080142E-4	0.0
72-73	35.42735513694776	1.017586126963407E-5	0.0	5.596723698298739E-4	0.0
74-75	35.84759785566111	1.017586126963407E-5	0.0	5.825680576865505E-4	0.0
76-77	36.25228931438914	1.017586126963407E-5	0.0	6.156396068128612E-4	0.0
78-79	36.64053912527075	1.017586126963407E-5	0.0	6.716068437958487E-4	0.0
80-81	37.01668474917214	1.017586126963407E-5	0.0	7.886292483966404E-4	0.0
82-83	37.38816219671609	1.017586126963407E-5	0.0	8.089809709359085E-4	0.0
84-85	37.75854573917354	1.017586126963407E-5	0.0	8.191568322055427E-4	0.0
86-87	38.127115434359695	1.017586126963407E-5	0.0	8.217007975229512E-4	5.087930634817035E-6
88	38.40173649037394	1.017586126963407E-5	0.0	8.395085547448107E-4	1.017586126963407E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	8775	0.0	47.66915	9
GCTTGCT	2195	0.0	43.0384	7
CCTGCTG	6810	0.0	41.340366	8
GCCTGCT	2110	0.0	40.985474	7
CTTGCTG	6230	0.0	39.455563	8
CTGCTGA	8630	0.0	39.266216	9
GTTGCTG	2680	0.0	38.93254	8
TGAAAAA	15565	0.0	38.228466	1
CATGCTG	1245	0.0	30.200573	8
TACCCGA	14815	0.0	29.37676	6
GACACGC	1275	0.0	29.121645	2
ACCCGAC	15685	0.0	28.526402	7
ATGCTGA	2365	0.0	28.418388	9
TGCCCGA	1720	0.0	28.418316	5
GCTGAAC	17970	0.0	28.325327	10-11
GTACGAC	1130	0.0	28.282972	6
GTTTGCC	900	0.0	28.199715	3
ACTTGCT	1815	0.0	27.96673	7
TGAACCG	15850	0.0	27.903221	12-13
CCCTGCT	4020	0.0	27.241089	7
>>END_MODULE