##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248459_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19654356
Sequences flagged as poor quality	0
Sequence length	100
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14216899297031	34.0	31.0	34.0	30.0	34.0
2	32.44056503301355	34.0	31.0	34.0	30.0	34.0
3	32.57938448860904	34.0	31.0	34.0	31.0	34.0
4	36.10488753739883	37.0	35.0	37.0	35.0	37.0
5	35.97575860536972	37.0	35.0	37.0	35.0	37.0
6	35.88180141847436	37.0	35.0	37.0	35.0	37.0
7	35.91589920320971	37.0	35.0	37.0	35.0	37.0
8	35.763915744682755	37.0	35.0	37.0	33.0	37.0
9	37.44760357449514	39.0	37.0	39.0	34.0	39.0
10-11	37.62711718969576	39.0	37.0	39.0	35.0	39.0
12-13	37.679682025704636	39.0	37.0	39.0	35.0	39.0
14-15	39.12860930167338	41.0	39.0	41.0	36.0	41.0
16-17	39.01698842231208	40.5	38.5	41.0	35.5	41.0
18-19	38.68562221016043	40.0	38.0	41.0	34.5	41.0
20-21	38.24063808043367	40.0	36.5	41.0	34.0	41.0
22-23	37.83216695067495	39.5	35.5	41.0	34.0	41.0
24-25	37.47766523105616	39.0	35.0	41.0	33.0	41.0
26-27	37.26743443031153	39.0	35.0	41.0	33.0	41.0
28-29	37.09096914699215	39.0	35.0	41.0	33.0	41.0
30-31	37.038391972751484	39.0	35.0	41.0	33.0	41.0
32-33	36.95865031141189	39.0	35.0	41.0	33.0	41.0
34-35	36.89433866467057	38.5	35.0	41.0	33.0	41.0
36-37	36.875269584004684	38.5	35.0	41.0	33.0	41.0
38-39	36.762431824273456	38.0	35.0	41.0	33.0	41.0
40-41	36.62393273023039	38.0	35.0	41.0	32.0	41.0
42-43	36.46677675422181	38.0	35.0	41.0	32.0	41.0
44-45	36.29287609321821	37.5	35.0	40.0	31.0	41.0
46-47	36.04340048078909	37.0	35.0	40.0	31.0	41.0
48-49	35.72911297627864	36.5	35.0	40.0	31.0	41.0
50-51	35.40965061383848	35.5	35.0	39.0	30.0	41.0
52-53	35.087480098559325	35.0	35.0	39.0	30.0	41.0
54-55	34.81610758449679	35.0	35.0	38.5	30.0	40.0
56-57	34.49994494859053	35.0	34.0	37.0	29.5	40.0
58-59	34.19232141210834	35.0	34.0	36.5	29.0	40.0
60-61	33.935317112399915	35.0	34.0	36.0	29.0	39.0
62-63	33.68894333144266	35.0	33.5	35.0	28.0	39.0
64-65	33.498254203800926	35.0	33.0	35.0	28.5	38.5
66-67	33.32830582187481	35.0	33.0	35.0	28.0	37.5
68-69	33.16640074597204	35.0	33.0	35.0	28.0	37.0
70-71	33.02577662682003	35.0	33.0	35.0	27.0	36.5
72-73	32.87806102626817	35.0	33.0	35.0	27.0	36.0
74-75	32.7575207501075	35.0	33.0	35.0	26.5	36.0
76-77	32.628960241688915	35.0	33.0	35.0	27.0	35.0
78-79	32.509928409763205	35.0	33.0	35.0	27.0	35.0
80-81	32.41371157111431	35.0	33.0	35.0	27.0	35.0
82-83	32.295559925748776	35.0	33.0	35.0	26.0	35.0
84-85	32.16179441341146	35.0	33.0	35.0	25.0	35.0
86-87	32.01505483059328	35.0	32.0	35.0	25.0	35.0
88-89	31.894865519887805	35.0	32.0	35.0	25.0	35.0
90-91	31.77995669255202	35.0	32.0	35.0	24.5	35.0
92-93	31.617786815299368	34.0	32.0	35.0	24.0	35.0
94-95	31.41531910279838	34.0	31.5	35.0	23.5	35.0
96-97	31.157063223033106	34.0	31.0	35.0	21.5	35.0
98-99	30.889753599660047	34.0	31.0	35.0	20.0	35.0
100	29.60273254437846	33.0	29.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33372392407155616
1101	2	0.3268809494492686
1101	3	0.315607600725329
1101	4	0.2205262578730327
1101	5	0.18249446274237613
1101	6	0.18934216304562312
1101	7	0.21749132559215667
1101	8	0.15504313592875008
1101	9	0.21003155490043923
1101	10-11	0.200954272997798
1101	12-13	0.2574755651379874
1101	14-15	0.36182634125945157
1101	16-17	0.4259856645735667
1101	18-19	0.46681242010705404
1101	20-21	0.44441207051797704
1101	22-23	0.4264497192111989
1101	24-25	0.38913716962037626
1101	26-27	0.43959826901709675
1101	28-29	0.47118620195345784
1101	30-31	0.4803760913244659
1101	32-33	0.46406752496753256
1101	34-35	0.5029289609198955
1101	36-37	0.4961082369595431
1101	38-39	0.4902543040544245
1101	40-41	0.49693650454061356
1101	42-43	0.5539577933689657
1101	44-45	0.5315637518496032
1101	46-47	0.5245849857152081
1101	48-49	0.533023396219555
1101	50-51	0.5248084332787002
1101	52-53	0.499737313368243
1101	54-55	0.5153394021024624
1101	56-57	0.5051748478762832
1101	58-59	0.4777227738985843
1101	60-61	0.4947118288646024
1101	62-63	0.500066390351904
1101	64-65	0.49772421670478906
1101	66-67	0.468208721223462
1101	68-69	0.4912148459854748
1101	70-71	0.49035912787425673
1101	72-73	0.49878955718685347
1101	74-75	0.46264677132327847
1101	76-77	0.4680388646235514
1101	78-79	0.45332230555900566
1101	80-81	0.41948190651477546
1101	82-83	0.35606817237863453
1101	84-85	0.3438053583364038
1101	86-87	0.4321035157827424
1101	88-89	0.3503928209352303
1101	90-91	0.32469785561590925
1101	92-93	0.3411361624085565
1101	94-95	0.3376452022648344
1101	96-97	0.3530097871572693
1101	98-99	0.3112364062319628
1101	100	0.03813896538986228
1102	1	0.3169947292388571
1102	2	0.30190100773283746
1102	3	0.30267994223118677
1102	4	0.21854600427241877
1102	5	0.20218769828128558
1102	6	0.16225373834949863
1102	7	0.22374103852244787
1102	8	-0.00567411121332384
1102	9	0.13144480400806913
1102	10-11	0.2134989864435326
1102	12-13	0.24511883331207684
1102	14-15	0.3652601345819235
1102	16-17	0.4318009353436594
1102	18-19	0.4504401607035149
1102	20-21	0.3999785181606299
1102	22-23	0.3200848658480382
1102	24-25	0.3218053169948192
1102	26-27	0.34840244710599677
1102	28-29	0.35091332081174187
1102	30-31	0.38792441865070515
1102	32-33	0.4186822264874337
1102	34-35	0.4209350408049275
1102	36-37	0.4232862181698209
1102	38-39	0.4171428321697519
1102	40-41	0.4335167918560714
1102	42-43	0.4462425275843813
1102	44-45	0.4493382038110312
1102	46-47	0.449832477528588
1102	48-49	0.4348895638214998
1102	50-51	0.4474188775198158
1102	52-53	0.441473501026735
1102	54-55	0.41172702340509915
1102	56-57	0.43403221479664467
1102	58-59	0.41954495611979326
1102	60-61	0.4161741593389081
1102	62-63	0.42866438100377025
1102	64-65	0.39559458919250545
1102	66-67	0.40856331793212064
1102	68-69	0.37677671864903317
1102	70-71	0.3723516251575347
1102	72-73	0.3560953821732511
1102	74-75	0.3247435518868258
1102	76-77	0.3812138615290763
1102	78-79	0.3719082740757429
1102	80-81	0.3292955773606039
1102	82-83	0.3564072944146375
1102	84-85	0.37509425255070283
1102	86-87	0.41641980554568647
1102	88-89	0.36394904874276435
1102	90-91	0.35660157004885207
1102	92-93	0.3497462801008524
1102	94-95	0.29946462140303964
1102	96-97	0.22071694011060217
1102	98-99	0.23508819585716623
1102	100	0.19745679509941283
1103	1	0.23240145233987164
1103	2	0.24359538963446425
1103	3	0.24141250988130025
1103	4	0.15663726215596796
1103	5	0.17534619156748477
1103	6	0.06556515525394246
1103	7	0.1475771442846323
1103	8	0.1251976558115402
1103	9	0.1434782283683802
1103	10-11	0.19554774030984134
1103	12-13	0.22283802546352405
1103	14-15	0.3218484259854222
1103	16-17	0.35745855856891495
1103	18-19	0.38431375643525456
1103	20-21	0.3377469317382449
1103	22-23	0.2836702849495438
1103	24-25	0.27881495754800056
1103	26-27	0.2913292137207577
1103	28-29	0.31478988381725515
1103	30-31	0.31149840388449235
1103	32-33	0.3249194297589142
1103	34-35	0.33256212051177414
1103	36-37	0.3530466893192141
1103	38-39	0.327817674645992
1103	40-41	0.34630865126024446
1103	42-43	0.34623076935064745
1103	44-45	0.34324892469986423
1103	46-47	0.3290353151934653
1103	48-49	0.33126768116984806
1103	50-51	0.3144204668187669
1103	52-53	0.3022169992636137
1103	54-55	0.27843317692900627
1103	56-57	0.24380709814078472
1103	58-59	0.2662998365356586
1103	60-61	0.24313676036906884
1103	62-63	0.24853706171435874
1103	64-65	0.2228504276721921
1103	66-67	0.22814762764214436
1103	68-69	0.17950359093490675
1103	70-71	0.19055162737009823
1103	72-73	0.2045935360996083
1103	74-75	0.15940047921262135
1103	76-77	0.16051566876783596
1103	78-79	0.1637631959922956
1103	80-81	0.08102915050289283
1103	82-83	0.10183735539140315
1103	84-85	0.15897907268602296
1103	86-87	0.22370147135069374
1103	88-89	0.140613204346419
1103	90-91	0.04463880082967009
1103	92-93	0.08750729621035447
1103	94-95	0.05201005396722991
1103	96-97	0.037755642965521474
1103	98-99	0.006469182157292863
1103	100	-0.06145723513223089
1104	1	0.18199848994279222
1104	2	0.1933700992358709
1104	3	0.19218091972661
1104	4	0.1356183921594436
1104	5	0.11953646413321195
1104	6	0.07426205953306919
1104	7	0.11305387387158561
1104	8	0.021647865869319105
1104	9	-0.017471336678184457
1104	10-11	0.10190002279167487
1104	12-13	0.16184336020010903
1104	14-15	0.2397229421628566
1104	16-17	0.2972933248157261
1104	18-19	0.3298972031769196
1104	20-21	0.28641037900058564
1104	22-23	0.25531619800020877
1104	24-25	0.24208485792488688
1104	26-27	0.2451807872883478
1104	28-29	0.24292849471778766
1104	30-31	0.24231894896923478
1104	32-33	0.24860384258019508
1104	34-35	0.2552371752088263
1104	36-37	0.23733865866736892
1104	38-39	0.24590100166101792
1104	40-41	0.24112616713196644
1104	42-43	0.27551785508752147
1104	44-45	0.25477704165232495
1104	46-47	0.25672961324461596
1104	48-49	0.2757825412513313
1104	50-51	0.28631153609433824
1104	52-53	0.28163461479646656
1104	54-55	0.24209291646671716
1104	56-57	0.25302140589953126
1104	58-59	0.2299408943252601
1104	60-61	0.22770666879063128
1104	62-63	0.20506941850403848
1104	64-65	0.2524659548332977
1104	66-67	0.22991740102629876
1104	68-69	0.2114947920214263
1104	70-71	0.18276365912331727
1104	72-73	0.21301527165037726
1104	74-75	0.1798073173320205
1104	76-77	0.19113142347595868
1104	78-79	0.1796382912566088
1104	80-81	0.1515166971829771
1104	82-83	0.13396498825983372
1104	84-85	0.16972125866332277
1104	86-87	0.2365080348873505
1104	88-89	0.16709981444923372
1104	90-91	0.13405682009359055
1104	92-93	0.09622250636033414
1104	94-95	0.08241657659599255
1104	96-97	0.0033083516050744777
1104	98-99	-0.04597296494950598
1104	100	-0.1471370357554207
1105	1	0.19948242558938745
1105	2	0.1783652094940109
1105	3	0.16609709199432388
1105	4	0.1173409989663412
1105	5	0.11210948712942326
1105	6	0.11238407856016863
1105	7	0.13136227736081452
1105	8	-0.003390690320941303
1105	9	0.1260523975711365
1105	10-11	0.15567014097212706
1105	12-13	0.13948646701343392
1105	14-15	0.21860069594483633
1105	16-17	0.24942027711211523
1105	18-19	0.25234162153321904
1105	20-21	0.24666481683059516
1105	22-23	0.22737546627480043
1105	24-25	0.18983793709998764
1105	26-27	0.19339704182605288
1105	28-29	0.19655271504439042
1105	30-31	0.1835803529677804
1105	32-33	0.202227021350879
1105	34-35	0.20553483890224555
1105	36-37	0.18960466975953238
1105	38-39	0.21020110122872637
1105	40-41	0.2048913052346748
1105	42-43	0.22539484222401285
1105	44-45	0.2077883174000661
1105	46-47	0.20432564400797304
1105	48-49	0.22239630167873514
1105	50-51	0.2453811357356912
1105	52-53	0.2179033007913702
1105	54-55	0.20070527552125839
1105	56-57	0.21548012324274168
1105	58-59	0.18744970826138996
1105	60-61	0.20266234895493085
1105	62-63	0.21060246764896817
1105	64-65	0.20644748855159634
1105	66-67	0.20257923033054226
1105	68-69	0.21950067036113552
1105	70-71	0.1867817584104472
1105	72-73	0.18313522004952887
1105	74-75	0.1839053135612545
1105	76-77	0.19015150218103116
1105	78-79	0.16037328821452235
1105	80-81	0.12992879852136952
1105	82-83	0.1145023795295117
1105	84-85	0.12548477944883984
1105	86-87	0.15647576192012025
1105	88-89	0.10482471196509024
1105	90-91	0.11891078167276703
1105	92-93	0.1596898407259033
1105	94-95	0.12121260541904633
1105	96-97	0.14365232089256708
1105	98-99	0.08350967828131672
1105	100	0.06626531309230188
1106	1	0.10240537584073195
1106	2	0.10393085643682554
1106	3	0.10880243272161039
1106	4	0.0835676193426309
1106	5	0.08747813712132313
1106	6	0.08677051440011496
1106	7	0.09380585297269306
1106	8	-8.457142049564936E-4
1106	9	0.049029200523037275
1106	10-11	0.10379732903779626
1106	12-13	0.10738521711644466
1106	14-15	0.15362090003168305
1106	16-17	0.18229113292995436
1106	18-19	0.17786673065233316
1106	20-21	0.13512354302117302
1106	22-23	0.15970396774819307
1106	24-25	0.15590895972230356
1106	26-27	0.1523978950295799
1106	28-29	0.1365833941404091
1106	30-31	0.13407575802988703
1106	32-33	0.15234250752769896
1106	34-35	0.15696664549145822
1106	36-37	0.1566519479621391
1106	38-39	0.15399640768578848
1106	40-41	0.1627503149502587
1106	42-43	0.16237251258765895
1106	44-45	0.1829153388743734
1106	46-47	0.16425212713286896
1106	48-49	0.14723019872313614
1106	50-51	0.1587588217994167
1106	52-53	0.19036304002793258
1106	54-55	0.1849113753226277
1106	56-57	0.15270105606913376
1106	58-59	0.1164011736970636
1106	60-61	0.13718508778798366
1106	62-63	0.09773998935202144
1106	64-65	0.08371869893553452
1106	66-67	0.13849015652168362
1106	68-69	0.12819867538515695
1106	70-71	0.08605039923804725
1106	72-73	0.09487625253235876
1106	74-75	0.09645049569640918
1106	76-77	0.06293480695584464
1106	78-79	0.07829372361117493
1106	80-81	0.07093183481769216
1106	82-83	0.004869304998877055
1106	84-85	0.03126769314243205
1106	86-87	0.09304349853668725
1106	88-89	0.06845200978924382
1106	90-91	0.015053143760695775
1106	92-93	0.04416136781102864
1106	94-95	0.044076746077568174
1106	96-97	0.018352358757471876
1106	98-99	0.023846979614901898
1106	100	-0.07644194574450225
1107	1	0.1431006182558079
1107	2	0.12553150497558363
1107	3	0.13186416618096786
1107	4	0.09146956314125987
1107	5	0.10252781165395675
1107	6	0.1227312607167832
1107	7	0.12927462526285893
1107	8	0.08739367202825576
1107	9	0.11618636438530672
1107	10-11	0.11703717140176195
1107	12-13	0.1220876539356226
1107	14-15	0.15698260779625173
1107	16-17	0.16540502778124733
1107	18-19	0.20841501737702828
1107	20-21	0.1863620757218598
1107	22-23	0.18307075285049734
1107	24-25	0.1761322414071813
1107	26-27	0.19858173989031513
1107	28-29	0.20349388937516721
1107	30-31	0.21783931389168032
1107	32-33	0.1954505491025884
1107	34-35	0.22672975665158646
1107	36-37	0.220106822412923
1107	38-39	0.19790845631256104
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2216	50-51	-0.6330626343211705
2216	52-53	-0.5564723749087008
2216	54-55	-0.4742622392969409
2216	56-57	-0.44047575898829194
2216	58-59	-0.42921205845723875
2216	60-61	-0.45950813184233397
2216	62-63	-0.46959103977252425
2216	64-65	-0.4219971749168394
2216	66-67	-0.36237329267297014
2216	68-69	-0.42038479151147357
2216	70-71	-0.3786433839229062
2216	72-73	-0.3812093363753064
2216	74-75	-0.3943236923258695
2216	76-77	-0.4345079882722729
2216	78-79	-0.3399201238053493
2216	80-81	-0.3263649360477032
2216	82-83	-0.40106883559681705
2216	84-85	-0.3985264488005029
2216	86-87	-0.41142400614286956
2216	88-89	-0.4941226235408003
2216	90-91	-0.47299649823160195
2216	92-93	-0.49006611975693914
2216	94-95	-0.5291789160866571
2216	96-97	-0.4385986489195126
2216	98-99	-0.41220252179719097
2216	100	-0.30264233375554994
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	8.0
7	103.0
8	474.0
9	1127.0
10	2190.0
11	3637.0
12	5598.0
13	8071.0
14	11033.0
15	15432.0
16	21934.0
17	31379.0
18	42283.0
19	55150.0
20	70033.0
21	86521.0
22	104696.0
23	127157.0
24	152362.0
25	179629.0
26	212140.0
27	251041.0
28	301055.0
29	369086.0
30	416394.0
31	510284.0
32	655173.0
33	924477.0
34	1314268.0
35	5003839.0
36	3170376.0
37	4179937.0
38	1245908.0
39	181232.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.42688248355493	22.884226592666117	31.55276493967447	27.136125984104474
2	15.703334729339987	23.24897033273007	39.02062814804292	22.027066789887023
3	12.307307347032893	20.65821948070952	42.649492051533	24.384981120724586
4	12.591025623022196	17.166255663629986	46.93015634803807	23.312562365309756
5	12.079849169990739	18.538946991564025	49.119529871820646	20.261673966624592
6	10.975220963739538	13.686284099056717	45.44701439212763	29.891480545076117
7	15.461015359648517	14.305556488342837	43.410320846941005	26.82310730506764
8	11.142496859220419	14.028020048074838	44.27772652535651	30.551756567348225
9	10.870928561587059	12.588323931855106	48.171092454008665	28.369655052549163
10-11	14.414483995303637	18.973002727741374	48.425740838315946	18.18677243863905
12-13	12.89882983102817	26.443824801859293	42.74936259628343	17.9079827708291
14-15	14.723667082927655	24.03821731754255	42.5906273302746	18.64748826925519
16-17	20.805731309639448	24.53923445774565	42.9534780992061	11.701556133408797
18-19	20.82078904035319	18.796250561453146	42.409792007430816	17.973168390762844
20-21	14.92494825379403	17.921719017828096	48.253773676800485	18.899559051577388
22-23	14.842799947960634	19.438973080311836	49.773164730801845	15.945062240925687
24-25	15.092009950162439	21.13515304896271	47.37248769045054	16.400349310424314
26-27	19.352449239100395	21.031444628562053	43.264325354134684	16.351780778202862
28-29	17.146066060269128	24.00563219229152	46.22628590280271	12.622015844636644
30-31	17.255765242521313	21.46415626408657	46.390789400410505	14.889289092981613
32-33	18.102440459203255	22.542649943937988	45.746601134041605	13.608308462817151
34-35	18.129381307535457	22.647736495047592	45.79388752576668	13.428994671650276
36-37	18.51854584340445	21.692277825265133	45.36032157290124	14.428854758429177
38-39	19.06969773461107	21.789702590713173	44.533872965813984	14.60672670886177
40-41	19.112064504838276	22.18736391601032	44.75441782517123	13.946153753980168
42-43	19.11366985630133	22.670168560327273	44.86739982996462	13.348761753406777
44-45	19.470693055775268	21.81050687447531	44.53940829713865	14.179391772610773
46-47	19.903617687326193	21.234775563927506	44.84627712619289	14.015329622553407
48-49	19.029372440718575	21.343121984397424	45.10427477355417	14.523230801329836
50-51	18.93666631735434	21.11688405062062	45.27113416521567	14.675315466809364
52-53	18.60260537126815	21.484207602954836	45.40009488657625	14.513092139200765
54-55	18.318700858821874	21.79021134940943	45.39347809489423	14.49760969687447
56-57	18.043385390944973	22.04246300986258	44.77587482971211	15.138276769480338
58-59	17.786096399534685	22.181848017101398	45.184970256361986	14.847085327001933
60-61	17.180515043759865	21.68216661566616	45.513849564334876	15.623468776239102
62-63	17.01294100814247	21.491791382416924	45.63241992822446	15.862847681216142
64-65	16.90412036882981	21.53363184161619	45.49728891101871	16.064958878535297
66-67	16.28381820190665	21.31822893681752	45.983496920736386	16.414455940539437
68-69	16.120455109009153	20.91889612327183	46.33430074156941	16.62634802614961
70-71	15.666081084570461	20.590595435359788	46.94918766225036	16.794135817819395
72-73	15.421699264625225	20.316977097409634	47.58408077338803	16.67724286457711
74-75	15.25973619595755	19.972576761713338	47.961388174111356	16.806298868217752
76-77	15.223183728411074	19.84470978847152	48.00550598947311	16.926600493644294
78-79	15.140719725565402	19.899993525565936	48.12154280802947	16.837743940839193
80-81	14.979642914019422	19.50733409687246	48.59957647492201	16.913446514186106
82-83	15.110059102714537	19.336003424804947	48.9909056856789	16.56303178680162
84-85	15.124022727143792	19.36348255132976	49.13235110837705	16.380143613149396
86-87	15.26732840616584	19.467296972615316	49.08409970632679	16.18127491489206
88-89	15.257733354014139	19.956207537431194	48.99867054507245	15.787388563482216
90-91	15.283909560107507	20.404637892847287	48.89632751609462	15.415125030950586
92-93	15.111997722845128	20.76497770811607	48.62184329113292	15.501181277905877
94-95	15.025587183367525	21.43418071392972	48.35575835453801	15.184473748164745
96-97	14.487311734601413	22.381265434915978	48.517149069959345	14.61427376052327
98-99	14.154769104304634	23.32278638483535	48.2797392249303	14.242705285929713
100	13.843278655326557	24.334159562562377	47.69846864776813	14.124093134342939
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	649116.0
1	1333933.0
2	1801692.5
3	1053094.0
4	321378.0
5	89190.5
6	45998.5
7	30890.0
8	25809.0
9	25900.0
10	29520.0
11	35352.5
12	44586.5
13	58383.0
14	75609.0
15	95859.0
16	120298.5
17	148080.5
18	175194.5
19	197062.0
20	212140.5
21	222157.5
22	230588.0
23	238341.0
24	244700.5
25	247557.5
26	244782.0
27	238623.5
28	229402.0
29	216173.0
30	201381.0
31	186214.0
32	170144.5
33	154157.0
34	139154.5
35	125100.5
36	111599.5
37	99679.0
38	88614.5
39	78301.5
40	70411.5
41	64691.5
42	62192.0
43	65444.0
44	78956.0
45	108863.5
46	159455.5
47	230615.0
48	321636.0
49	435936.5
50	579931.0
51	755686.0
52	945945.0
53	1108658.0
54	1185751.5
55	1134547.5
56	958610.5
57	707300.0
58	456360.0
59	260016.5
60	132317.5
61	62054.5
62	27751.0
63	12564.0
64	5953.0
65	3217.5
66	2059.5
67	1492.5
68	1123.0
69	885.0
70	691.0
71	496.0
72	391.0
73	300.5
74	230.0
75	160.5
76	113.5
77	80.0
78	47.5
79	37.5
80	31.5
81	26.0
82	20.0
83	16.5
84	17.0
85	12.5
86	9.0
87	10.0
88	9.0
89	5.0
90	3.5
91	5.5
92	5.5
93	3.5
94	2.0
95	1.0
96	1.5
97	1.5
98	0.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0416955915523256
2	0.003444529039771133
3	0.0
4	0.0
5	0.007647159744130004
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.631895952225552E-5
14-15	1.0175861269634071E-4
16-17	0.0
18-19	0.0
20-21	8.395085547448107E-5
22-23	2.5439653174085177E-5
24-25	0.0010404818148200836
26-27	0.0012821585199738928
28-29	0.0010048663003763645
30-31	0.0011066249130727052
32-33	0.001154960254103467
34-35	0.001701912797346298
36-37	0.002978983386685374
38-39	0.0037294531553208866
40-41	0.004184822947137011
42-43	0.004065256577218811
44-45	0.00583585643813514
46-47	0.005927439189561846
48-49	0.006392984842647604
50-51	0.007410570969611011
52-53	0.007214685640170556
54-55	0.005573828010442062
56-57	0.004446851374830089
58-59	0.005054859085690725
60-61	0.0048742375481547195
62-63	0.004395972068481919
64-65	0.0032053962999347317
66-67	0.001971573120991601
68-69	0.0015034835025884339
70-71	6.283594333999038E-4
72-73	8.140689015707257E-4
74-75	0.0011295206009293817
76-77	6.258154680824953E-4
78-79	6.53799086573989E-4
80-81	0.0013533895488613314
82-83	4.986172022120694E-4
84-85	0.0012032955951342288
86-87	0.0010023223350589558
88-89	3.078198034064306E-4
90-91	2.1623705197972397E-4
92-93	2.798361849149369E-5
94-95	4.197542773724054E-4
96-97	2.747482542801199E-4
98-99	0.0016790171094896217
100	0.004126311744836615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9654356E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.55114259530127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.98263486645858	65.36599639079195
2	5.198859895102777	7.231732917945726
3	0.5391549385600549	1.1249652603825382
4	0.10254145754118749	0.2852750214150865
5	0.04048754718421662	0.14079775837717196
6	0.019716751842793808	0.08227935713605929
7	0.01759682904259748	0.08567156961767852
8	0.010252505219197723	0.05704587619675931
9	0.01089756749615759	0.06821444437804591
>10	0.05909680814806954	0.8119445227633013
>50	0.008255562458107336	0.40589531766670583
>100	0.007552638819688547	1.0901027906305683
>500	9.611059360455345E-4	0.4535279544958218
>1k	9.699389102456145E-4	1.5356778633454076
>5k	2.6650102965434895E-4	1.3326525965611484
>10k+	7.55086250687322E-4	19.92822035829596
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117925	0.5999942201107988	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100083	0.5092153617243933	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97442	0.4957781369178415	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97127	0.4941754387678741	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90572	0.4608240534566485	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89275	0.4542250074232908	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81793	0.4161571104135897	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79957	0.4068156697680657	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79947	0.40676479046171754	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75357	0.3834111888479073	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73032	0.3715817501219577	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72970	0.37126629842259906	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72469	0.36871724517455573	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72449	0.3686154865618594	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69582	0.35402838943183895	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69488	0.3535501239521661	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66686	0.3392937423134088	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66455	0.3381184303367661	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63941	0.32532737272083606	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62193	0.31643366997117583	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61886	0.314871675266287	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61779	0.3143272666883616	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61623	0.31353354950933016	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60036	0.3054590035918755	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59317	0.3018007814654421	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59201	0.30121058151180324	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56107	0.28546852412767937	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55998	0.28491393968848433	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55347	0.28160169684521846	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55279	0.28125571756205087	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53348	0.27143092350621917	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53010	0.269711202951651	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48923	0.24891683044715382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47920	0.2438136360204323	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47088	0.23958047773226457	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44860	0.22824456827789222	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40850	0.2078419664322759	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38618	0.19648570525536424	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36501	0.1857145561014566	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33447	0.17017601594272536	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33286	0.16935685911051984	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33173	0.1687819229487855	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32905	0.16741835753865453	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32536	0.16554091113440705	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31993	0.1627781647997014	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31403	0.15977628572515934	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30952	0.15748162900885687	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29515	0.15017027268662478	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29061	0.14786035217841786	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28517	0.1450925179130774	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26993	0.13733851162561622	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26553	0.13509982214629673	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25829	0.13141616036668918	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25466	0.12956924154625063	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25351	0.12898412952324667	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25056	0.12748318998597563	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24605	0.12518853326967314	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24589	0.12510712637951607	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24355	0.12391655061096889	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24104	0.12263948002162982	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23648	0.12031938365215324	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23177	0.11792296832315442	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22412	0.11403070138751939	No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22253	0.11322172041658349	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22212	0.11301311526055599	No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21973	0.11179709983883471	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21259	0.10816431736557534	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20751	0.1055796486030883	No Hit
ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20558	0.10459767799056861	No Hit
GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19944	0.10147368858079095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002315008438841751	0.0	0.0	1.017586126963407E-5	0.0
2	0.005530580600046117	0.0	0.0	1.017586126963407E-5	0.0
3	0.00907686825251359	0.0	0.0	2.035172253926814E-5	0.0
4	0.015335022933338544	0.0	0.0	3.052758380890221E-5	0.0
5	0.025271751463136212	0.0	0.0	3.052758380890221E-5	0.0
6	0.045562418834786546	0.0	5.087930634817035E-6	3.052758380890221E-5	0.0
7	0.06985728761603789	0.0	5.087930634817035E-6	3.052758380890221E-5	0.0
8	0.1857450836852655	0.0	5.087930634817035E-6	3.052758380890221E-5	0.0
9	0.6428091564027842	0.0	5.087930634817035E-6	3.052758380890221E-5	0.0
10-11	1.531551071935402	0.0	5.087930634817035E-6	3.052758380890221E-5	0.0
12-13	2.463237665991193	0.0	5.087930634817035E-6	3.052758380890221E-5	0.0
14-15	3.4587498058954464	0.0	5.087930634817035E-6	3.561551444371924E-5	0.0
16-17	4.430473326116612	0.0	5.087930634817035E-6	3.561551444371924E-5	0.0
18-19	5.486666670737011	0.0	5.087930634817035E-6	3.561551444371924E-5	0.0
20-21	6.860234952496027	0.0	5.087930634817035E-6	3.561551444371924E-5	0.0
22-23	8.476543316911528	0.0	5.087930634817035E-6	3.561551444371924E-5	0.0
24-25	10.326794731915918	0.0	5.087930634817035E-6	4.070344507853628E-5	0.0
26-27	12.312932054349682	0.0	5.087930634817035E-6	4.070344507853628E-5	0.0
28-29	14.548128669288376	0.0	5.087930634817035E-6	4.3247410395944795E-5	0.0
30-31	16.968121468848942	0.0	5.087930634817035E-6	4.5791375713353315E-5	0.0
32-33	19.706646709767544	0.0	5.087930634817035E-6	4.5791375713353315E-5	0.0
34-35	22.30365370404403	0.0	5.087930634817035E-6	4.5791375713353315E-5	0.0
36-37	24.27794632395994	0.0	5.087930634817035E-6	7.12310288874385E-5	0.0
38-39	25.73044621762219	0.0	5.087930634817035E-6	9.412671674411515E-5	0.0
40-41	27.105400960479194	0.0	1.017586126963407E-5	1.2719826587042588E-4	0.0
42-43	28.229708976473205	0.0	1.017586126963407E-5	1.3991809245746844E-4	0.0
44-45	29.12273238563502	0.0	1.017586126963407E-5	1.5009395372710252E-4	0.0
46-47	29.868938468398557	0.0	1.017586126963407E-5	1.577258496793281E-4	0.0
48-49	30.535187721235943	0.0	1.017586126963407E-5	1.7044567626637067E-4	0.0
50-51	31.185811939093806	5.087930634817035E-6	1.017586126963407E-5	2.0097326007527288E-4	0.0
52-53	31.87634842881649	5.087930634817035E-6	1.2719826587042589E-5	2.1369308666231547E-4	0.0
54-55	32.53892419573555	5.087930634817035E-6	1.5263791904451105E-5	2.340448092015836E-4	0.0
56-57	33.19655958200818	5.087930634817035E-6	1.5263791904451105E-5	2.442206704712177E-4	0.0
58-59	33.819426594287805	5.087930634817035E-6	1.5263791904451105E-5	2.518525664234432E-4	0.0
60-61	34.42329527357701	5.087930634817035E-6	3.052758380890221E-5	2.6202842769307733E-4	0.0
62-63	35.021480734347136	5.087930634817035E-6	3.307154912631073E-5	2.7220428896271136E-4	0.0
64-65	35.60343060846155	5.087930634817035E-6	3.561551444371924E-5	2.95099976819388E-4	0.0
66-67	36.13562306493279	5.087930634817035E-6	3.561551444371924E-5	3.2817152594569876E-4	0.0
68-69	36.6113776508373	5.087930634817035E-6	3.561551444371924E-5	3.5106721380237537E-4	0.0
70-71	37.05437105138424	5.087930634817035E-6	3.561551444371924E-5	3.841387629286861E-4	0.0
72-73	37.4890253336207	1.017586126963407E-5	3.561551444371924E-5	4.0957841610277134E-4	0.0
74-75	37.90543938453135	1.017586126963407E-5	3.561551444371924E-5	4.4264996522908203E-4	0.0
76-77	38.3059104047978	1.017586126963407E-5	3.561551444371924E-5	5.011611675294779E-4	0.0
78-79	38.68572188272157	1.017586126963407E-5	3.561551444371924E-5	5.774801270517335E-4	0.0
80-81	39.049949029110905	1.017586126963407E-5	3.561551444371924E-5	6.792387397480742E-4	0.0
82-83	39.41052812923506	1.017586126963407E-5	3.561551444371924E-5	7.046783929221594E-4	0.0
84-85	39.77032114407615	1.017586126963407E-5	3.561551444371924E-5	7.072223582395679E-4	0.0
86-87	40.128623395241235	1.017586126963407E-5	3.561551444371924E-5	7.123102888743849E-4	0.0
88	40.39533017515303	1.017586126963407E-5	3.561551444371924E-5	7.123102888743849E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	4940	0.0	59.678257	1
GACTTTT	4265	0.0	55.01195	1
TGCCCTA	14405	0.0	54.74665	6
GCATTTT	3140	0.0	51.960747	1
GCCCTAC	38815	0.0	50.66067	7
CACTTTT	7165	0.0	49.74261	1
CCCCTAC	90945	0.0	48.881577	7
GCCCCTA	11310	0.0	48.577003	6
AGCTTTT	3025	0.0	48.495872	1
TGCTCTA	13530	0.0	47.65793	6
CCCCCTA	47210	0.0	47.56538	6
CGCCCTA	15565	0.0	45.5335	6
ATGCCCT	4860	0.0	44.776382	5
CCGCCTA	6810	0.0	44.030376	6
CTCCCTA	30450	0.0	43.262802	6
GGCCCTA	5935	0.0	42.7614	6
CACCCTA	18540	0.0	42.637833	6
TCCCCTA	28535	0.0	42.5592	6
ACCTTTT	7735	0.0	41.943462	1
CATTTTT	27790	0.0	41.58808	1
>>END_MODULE