##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248458_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11230949
Sequences flagged as poor quality	0
Sequence length	100
%GC	28
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.867894244733904	31.0	28.0	33.0	18.0	34.0
2	29.450738223457343	31.0	30.0	33.0	23.0	34.0
3	29.80766754439006	31.0	30.0	34.0	25.0	34.0
4	33.26252171566267	35.0	33.0	37.0	27.0	37.0
5	33.04596895596267	35.0	33.0	37.0	26.0	37.0
6	32.849749117372006	35.0	32.0	37.0	25.0	37.0
7	32.986639152221244	35.0	32.0	37.0	27.0	37.0
8	32.82707961722558	35.0	32.0	37.0	25.0	37.0
9	34.0727103292874	37.0	32.0	39.0	25.0	39.0
10-11	34.44462511582948	37.0	34.0	39.0	27.0	39.0
12-13	34.35766029210889	37.0	33.5	39.0	27.0	39.0
14-15	35.116186441591	38.0	32.5	40.0	27.0	41.0
16-17	34.90162207129602	37.5	32.0	40.0	27.0	41.0
18-19	34.34871051413376	36.5	32.0	39.5	26.0	41.0
20-21	33.96202694002083	35.5	32.0	39.0	25.5	40.5
22-23	33.41215150206808	35.0	32.0	38.5	25.0	40.0
24-25	32.88191202720269	35.0	30.5	38.0	24.5	40.0
26-27	32.40638440260035	35.0	30.0	37.0	23.0	40.0
28-29	32.20501539985624	35.0	30.0	37.0	23.5	40.0
30-31	32.096809851064236	35.0	30.0	37.0	23.0	40.0
32-33	31.908700547033025	35.0	30.0	37.0	22.0	40.0
34-35	31.739107532230804	35.0	29.5	37.0	22.0	40.0
36-37	31.554920648290718	34.0	28.0	37.0	22.0	39.0
38-39	31.35293135068105	34.0	27.5	37.0	21.0	39.0
40-41	31.104147165123802	34.0	27.0	36.5	20.5	39.0
42-43	30.86228532424108	33.0	26.0	36.0	20.0	39.0
44-45	30.64470420086495	33.0	26.0	35.0	20.0	39.0
46-47	30.503741001762187	33.0	26.0	35.0	20.0	39.0
48-49	30.3028156836969	33.0	25.5	35.0	20.0	39.0
50-51	30.090293527287855	33.0	26.0	35.0	20.0	38.5
52-53	29.810628068919197	33.0	26.0	35.0	18.5	38.0
54-55	29.608641620578993	33.0	26.0	35.0	18.0	37.5
56-57	29.398562178494444	32.0	26.0	35.0	17.5	37.0
58-59	29.114260112836412	32.0	26.0	35.0	16.5	36.5
60-61	28.85407702412325	31.0	26.0	35.0	15.0	36.0
62-63	28.802376406481766	31.0	26.0	35.0	16.5	35.0
64-65	29.051275586773656	31.5	26.0	35.0	18.5	35.0
66-67	29.50213904452776	32.0	26.5	35.0	20.0	35.0
68-69	29.52851887226983	32.0	27.0	35.0	20.0	35.0
70-71	29.839842251977103	33.0	28.0	35.0	20.0	35.0
72-73	29.865419298048636	33.0	28.0	35.0	20.0	35.0
74-75	29.802629813384428	33.0	28.0	35.0	20.0	35.0
76-77	29.709290862241474	33.0	28.0	35.0	20.0	35.0
78-79	29.613135719875498	33.0	27.5	35.0	19.5	35.0
80-81	29.543379415221278	33.0	27.0	35.0	19.0	35.0
82-83	29.46865540926239	33.0	27.0	35.0	19.0	35.0
84-85	29.39582305110637	33.0	27.0	35.0	18.0	35.0
86-87	29.26506611329105	33.0	27.0	35.0	17.5	35.0
88-89	29.145476041249943	33.0	27.0	35.0	16.5	35.0
90-91	28.96800181356001	33.0	27.0	35.0	15.0	35.0
92-93	28.775958914959013	33.0	27.0	35.0	11.0	35.0
94-95	28.50785254211376	33.0	26.0	35.0	10.0	35.0
96-97	28.197270640263792	33.0	25.0	35.0	10.0	35.0
98-99	27.818345181693907	32.0	25.0	35.0	8.0	35.0
100	26.526179844641803	31.0	23.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6874322697360924
1101	2	0.5937766314280388
1101	3	0.6135168490158378
1101	4	0.5571274177333976
1101	5	0.489078982508687
1101	6	0.3320724951933798
1101	7	0.37249457086632987
1101	8	0.42938286632234224
1101	9	0.4954298394066896
1101	10-11	0.48900400949452916
1101	12-13	0.6298431083977079
1101	14-15	0.7424896132477485
1101	16-17	0.7584998891979993
1101	18-19	0.7925993359637502
1101	20-21	0.6691771219062872
1101	22-23	0.5411990603616985
1101	24-25	0.5743506093351627
1101	26-27	0.6617096137537928
1101	28-29	0.5732460130382435
1101	30-31	0.4822129332037619
1101	32-33	0.5431425530121281
1101	34-35	0.5261487509905507
1101	36-37	0.44530041390173025
1101	38-39	0.3731003403719093
1101	40-41	0.4092899149728204
1101	42-43	0.34759237207259375
1101	44-45	0.3566770579762242
1101	46-47	0.39802029415870166
1101	48-49	0.3789997140156416
1101	50-51	0.41651019170504355
1101	52-53	0.28895708088234073
1101	54-55	0.29507714584686084
1101	56-57	0.2294061887056138
1101	58-59	0.298098808993398
1101	60-61	0.33724784447301204
1101	62-63	0.33052458059800927
1101	64-65	0.4284821783743453
1101	66-67	0.45675802335243176
1101	68-69	0.4422397670366358
1101	70-71	0.4307216233602773
1101	72-73	0.4019779988848988
1101	74-75	0.40027249167253487
1101	76-77	0.45139598987864105
1101	78-79	0.4406020976600544
1101	80-81	0.4153936935121294
1101	82-83	0.44950215928681914
1101	84-85	0.3801387475870506
1101	86-87	0.3229037206717287
1101	88-89	0.3611229045681448
1101	90-91	0.40727215216058
1101	92-93	0.3659608159154324
1101	94-95	0.36155401098924145
1101	96-97	0.2684495831152951
1101	98-99	0.2726120649910193
1101	100	0.21159598449922257
1102	1	0.39977670108747176
1102	2	0.34630300819725335
1102	3	0.31634774690657963
1102	4	0.3583510520399571
1102	5	0.3141556973860631
1102	6	0.16273523236152698
1102	7	0.1975464438968828
1102	8	0.1853780240671128
1102	9	0.22123398691118012
1102	10-11	0.20689363355455015
1102	12-13	0.3123299352978819
1102	14-15	0.4552614805752242
1102	16-17	0.4909324532602639
1102	18-19	0.5104241716194196
1102	20-21	0.3955015835196818
1102	22-23	0.2901994287847387
1102	24-25	0.2658632710878095
1102	26-27	0.4294521901356134
1102	28-29	0.27431964858538294
1102	30-31	0.17526259581763526
1102	32-33	0.13885001596200297
1102	34-35	0.10236488002194832
1102	36-37	0.09140169150337485
1102	38-39	0.10356716367108376
1102	40-41	0.11729589893763404
1102	42-43	0.1855052098296106
1102	44-45	0.14510412543382856
1102	46-47	0.12301806012816385
1102	48-49	0.1975093554249483
1102	50-51	0.08881731142694349
1102	52-53	0.10935988580932943
1102	54-55	0.05420402301287908
1102	56-57	0.031274639250330694
1102	58-59	0.025732850326743772
1102	60-61	0.14676007921491774
1102	62-63	0.1506128725570619
1102	64-65	0.19790052282463222
1102	66-67	0.16259452652711204
1102	68-69	0.18542688512144423
1102	70-71	0.2201440070731664
1102	72-73	0.22339242456314068
1102	74-75	0.18759534012365364
1102	76-77	0.23619763387170778
1102	78-79	0.23759913797855958
1102	80-81	0.20783564818302125
1102	82-83	0.14979703873434502
1102	84-85	0.07149327850950371
1102	86-87	0.14672197362417805
1102	88-89	0.12680599855581676
1102	90-91	0.18210748803315369
1102	92-93	0.15162204421801206
1102	94-95	0.12610704828014008
1102	96-97	0.09388167742582709
1102	98-99	0.17605764042725625
1102	100	0.16296824508029317
1103	1	0.45868654650619334
1103	2	0.382397440406379
1103	3	0.38657100611854744
1103	4	0.3743606885954023
1103	5	0.38506546342104286
1103	6	0.2554799379044965
1103	7	0.31545648790626757
1103	8	0.3051039546666203
1103	9	0.3271099093080281
1103	10-11	0.31901444488458
1103	12-13	0.3654266167785778
1103	14-15	0.5085384067232965
1103	16-17	0.5098338468388377
1103	18-19	0.5784745082468561
1103	20-21	0.420108726352197
1103	22-23	0.39142236391210616
1103	24-25	0.3503630496308219
1103	26-27	0.4573333643018671
1103	28-29	0.25290328962918807
1103	30-31	0.16179697742997945
1103	32-33	0.12989707416909724
1103	34-35	0.2069953592275624
1103	36-37	0.11780719363359182
1103	38-39	0.13095590956200098
1103	40-41	0.14566721146369233
1103	42-43	0.11828036927913743
1103	44-45	0.15174113572472692
1103	46-47	0.13587531198200153
1103	48-49	0.07380555225446273
1103	50-51	0.09260520513769777
1103	52-53	0.07264772221474658
1103	54-55	0.10699093186298114
1103	56-57	0.043791487775688864
1103	58-59	0.018971780374467784
1103	60-61	0.05712026519742608
1103	62-63	0.1713842735247475
1103	64-65	0.2173876361650109
1103	66-67	0.21222176644614166
1103	68-69	0.2176863716012427
1103	70-71	0.24127406092902248
1103	72-73	0.20826822405964762
1103	74-75	0.18680613387271805
1103	76-77	0.25153292108571534
1103	78-79	0.1731969773849933
1103	80-81	0.18607183763687374
1103	82-83	0.13002095282932302
1103	84-85	0.05291006417790811
1103	86-87	0.09527452787473933
1103	88-89	0.1377488482067939
1103	90-91	0.20070698415653254
1103	92-93	0.14684072934425174
1103	94-95	0.12379037194001796
1103	96-97	0.051613103592764276
1103	98-99	0.06704406183559897
1103	100	0.02596004301328847
1104	1	0.2536027441086688
1104	2	0.2157316769841664
1104	3	0.18070502517015186
1104	4	0.17730747690729487
1104	5	0.1867781394914232
1104	6	0.169348633569669
1104	7	0.19199399684527663
1104	8	0.2864643529413584
1104	9	0.16939956547712853
1104	10-11	0.17102167208607
1104	12-13	0.22778572621692206
1104	14-15	0.36049998893349766
1104	16-17	0.34945659589136113
1104	18-19	0.45993437242380253
1104	20-21	0.33650765890413226
1104	22-23	0.22368840491068198
1104	24-25	0.1930705497990104
1104	26-27	0.30786792928687845
1104	28-29	0.11822438680334812
1104	30-31	0.09113599248238557
1104	32-33	0.12483739462777166
1104	34-35	0.11833229183431371
1104	36-37	0.025694573377492702
1104	38-39	-0.006020940594105184
1104	40-41	0.1492188465230342
1104	42-43	0.10552934518680601
1104	44-45	0.005133363711102845
1104	46-47	-0.014819812491310813
1104	48-49	0.03434594783815825
1104	50-51	0.04328106558705258
1104	52-53	0.017348963210331192
1104	54-55	-0.014719389542541705
1104	56-57	-0.05557796972946605
1104	58-59	-0.05046619600911484
1104	60-61	-0.06983560165562253
1104	62-63	0.052844309006680135
1104	64-65	0.10729542113551815
1104	66-67	0.10303067791821974
1104	68-69	0.08733642355337068
1104	70-71	0.05402676692482089
1104	72-73	0.04408588454438345
1104	74-75	0.06881115279724881
1104	76-77	0.0859917125820111
1104	78-79	0.16989090023172082
1104	80-81	0.10978474543617267
1104	82-83	0.03039980158508726
1104	84-85	0.05923562524844428
1104	86-87	0.04008337954580199
1104	88-89	0.06669747477688048
1104	90-91	0.10949869847713245
1104	92-93	0.01470844639078095
1104	94-95	0.06240035036181979
1104	96-97	0.1124906896750737
1104	98-99	0.1752236878799387
1104	100	0.0726657236110988
1105	1	0.21733656227707243
1105	2	0.26869607487185476
1105	3	0.2600588409106095
1105	4	0.20755137520975353
1105	5	0.2153274011702493
1105	6	0.16739245736756914
1105	7	0.13730770747002907
1105	8	0.2313133324311778
1105	9	0.2221921476315032
1105	10-11	0.2809919833270911
1105	12-13	0.2899937852659491
1105	14-15	0.4368471186035734
1105	16-17	0.38296003904950027
1105	18-19	0.4201650405131119
1105	20-21	0.32603546838668507
1105	22-23	0.2111431933760386
1105	24-25	0.18097619649504537
1105	26-27	0.278167313255544
1105	28-29	0.11459323407731858
1105	30-31	0.12825822548885668
1105	32-33	0.14019101012234003
1105	34-35	0.1961907944592305
1105	36-37	0.023575463076223713
1105	38-39	-0.02509835601178878
1105	40-41	0.06752107529250395
1105	42-43	0.006974500128919203
1105	44-45	0.08613225298613614
1105	46-47	0.023986085270813362
1105	48-49	-0.011482421499763973
1105	50-51	0.022069900792722308
1105	52-53	0.0015903779281956076
1105	54-55	0.015094709077217772
1105	56-57	-0.09271183663527438
1105	58-59	-0.02774300366713689
1105	60-61	-0.05891086435561732
1105	62-63	0.034487845735881706
1105	64-65	0.10371166687917821
1105	66-67	0.09194746221323058
1105	68-69	0.06824509060010087
1105	70-71	0.07378945174314921
1105	72-73	0.0859401614752251
1105	74-75	0.09775039557619536
1105	76-77	0.03775987931751246
1105	78-79	0.06339594862993181
1105	80-81	0.13150093601551305
1105	82-83	0.12438782045402164
1105	84-85	0.060706368818262746
1105	86-87	0.09093147146729308
1105	88-89	0.11421907485162208
1105	90-91	0.12472890091292754
1105	92-93	0.12771254979502444
1105	94-95	0.15208674238911613
1105	96-97	0.1803241332973755
1105	98-99	0.10543057193488892
1105	100	0.06232293418884538
1106	1	0.007406884841895334
1106	2	0.14938016136899535
1106	3	0.11959749496014638
1106	4	0.14390100379101511
1106	5	0.16849210922660518
1106	6	0.06825251876431793
1106	7	0.17804844373821993
1106	8	0.20743526762172593
1106	9	0.23283065594505103
1106	10-11	0.25362315326010787
1106	12-13	0.31523562169174113
1106	14-15	0.35860508525436074
1106	16-17	0.2792696713880858
1106	18-19	0.3006951089716807
1106	20-21	0.20387425006410353
1106	22-23	0.1983828021145868
1106	24-25	0.14930349777267082
1106	26-27	0.23587445727446976
1106	28-29	0.10416155429982155
1106	30-31	0.16408220524712647
1106	32-33	0.11670174975193603
1106	34-35	0.08186858898129401
1106	36-37	0.049632577280622314
1106	38-39	0.05337397480966999
1106	40-41	0.07501238129157883
1106	42-43	0.08545448261426714
1106	44-45	0.03648480384888941
1106	46-47	0.01379908622477899
1106	48-49	-0.007902510224848669
1106	50-51	0.04084685035545377
1106	52-53	0.016166837163396508
1106	54-55	-0.018826788143439188
1106	56-57	-0.05216037212621316
1106	58-59	-0.017760665964427602
1106	60-61	0.08136423959272321
1106	62-63	0.12128169707298753
1106	64-65	0.11635557602390634
1106	66-67	0.12993384747002779
1106	68-69	0.09816154377492126
1106	70-71	0.12206137323185828
1106	72-73	0.13909804592939423
1106	74-75	0.12434471121439472
1106	76-77	0.1063431228219649
1106	78-79	0.12378153584801765
1106	80-81	0.19197657494346032
1106	82-83	0.11065974349535068
1106	84-85	0.1610591698246573
1106	86-87	0.15884540463184393
1106	88-89	0.22220844127430084
1106	90-91	0.19503581019362315
1106	92-93	0.1948159313143556
1106	94-95	0.16984293698787312
1106	96-97	0.19366584615507776
1106	98-99	0.16340940357260436
1106	100	0.10072209598338944
1107	1	-0.06576216673079927
1107	2	0.0365762346068621
1107	3	0.0640212075958182
1107	4	0.029616862348419204
1107	5	0.0631498734409277
1107	6	-0.008280934401625473
1107	7	-0.04785218200990471
1107	8	0.09403530544810224
1107	9	0.12175122343045786
1107	10-11	0.06612676917133342
1107	12-13	0.0469181745247198
1107	14-15	0.1847074972573708
1107	16-17	0.10600027192353423
1107	18-19	0.17135206911254386
1107	20-21	0.0773299517942263
1107	22-23	0.023134761999834552
1107	24-25	0.04171523500122731
1107	26-27	0.16636876820574287
1107	28-29	0.028670468849028907
1107	30-31	-0.0030579958946646
1107	32-33	0.008108310994234813
1107	34-35	-0.03476354376081048
1107	36-37	-0.0896579684886234
1107	38-39	-0.0758590677149904
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2216	34-35	0.923678238990604
2216	36-37	0.9453486262212003
2216	38-39	1.0133952036065175
2216	40-41	1.0036234218327849
2216	42-43	1.000943772713299
2216	44-45	0.9207522993731416
2216	46-47	0.8595159092581994
2216	48-49	0.881498507921755
2216	50-51	0.8159794160871314
2216	52-53	0.8487687646732383
2216	54-55	0.8609952693617728
2216	56-57	0.8463710151936574
2216	58-59	0.8328540756732288
2216	60-61	0.8006898499043089
2216	62-63	0.6980320235909474
2216	64-65	0.6050348225387125
2216	66-67	0.6209559279555386
2216	68-69	0.6092818631712262
2216	70-71	0.5592411645961164
2216	72-73	0.5299681057714345
2216	74-75	0.5356802750543004
2216	76-77	0.5312792554988661
2216	78-79	0.5007378550010877
2216	80-81	0.5484565621252315
2216	82-83	0.4938523691324832
2216	84-85	0.4184730114254869
2216	86-87	0.3321969412714303
2216	88-89	0.26942456090914035
2216	90-91	0.26271559652161613
2216	92-93	0.15165146027345244
2216	94-95	0.10208444500280933
2216	96-97	0.11910921482808234
2216	98-99	0.09613539111922265
2216	100	0.3067883538544187
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	220.0
6	2483.0
7	7488.0
8	12941.0
9	20131.0
10	28583.0
11	35331.0
12	37779.0
13	39975.0
14	44335.0
15	50460.0
16	59392.0
17	70264.0
18	84006.0
19	98513.0
20	114132.0
21	130649.0
22	148633.0
23	172573.0
24	236973.0
25	279268.0
26	306973.0
27	516550.0
28	433063.0
29	708987.0
30	678594.0
31	828287.0
32	1059866.0
33	1586658.0
34	1203136.0
35	2055233.0
36	147338.0
37	28474.0
38	3304.0
39	355.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.568360505391377	33.762316466178135	25.11885957492276	25.55046345350773
2	12.727728936319952	37.61813839252006	26.490495504543894	23.16363716661609
3	11.065905589822533	40.81194886015218	23.71071885504752	24.41142669497777
4	9.770550008975203	40.972076790695425	23.321948715976145	25.93542448435323
5	9.621464670748257	40.38277362851841	24.342173214660953	25.653588486072376
6	9.48937618717706	37.15249708639938	25.81289435113631	27.545232375287256
7	13.07105036270755	41.93305481130757	21.399901290621123	23.595993535363753
8	11.592911694283359	39.19888693288519	19.28612622139055	29.922075151440897
9	9.39029284168239	38.316192157937856	19.969327614255928	32.324187386123825
10-11	12.96730133847104	52.245495906000464	18.84424904787654	15.942953707651952
12-13	12.620784052629599	53.250130164943855	18.281212818959762	15.84787296346678
14-15	13.777580149282132	52.21974118126616	17.82378764252246	16.178891026929247
16-17	13.772905566573227	52.02811445408576	18.36158280123968	15.837397178101334
18-19	14.216723804907314	50.70025248979405	19.35582202358857	15.727201681710067
20-21	13.404239519335109	50.61487713008604	20.01255370987102	15.96832964070782
22-23	13.770529433490388	51.266691823343855	20.43595963582059	14.526819107345169
24-25	13.89299980687268	51.326759762523736	19.871706117381343	14.90853431322224
26-27	15.299631259092642	51.470734234529836	18.940494728793837	14.28913977758369
28-29	14.884240859788427	52.297971435895576	19.41851930767382	13.399268396642173
30-31	15.165339100017283	52.149497785093665	19.27939927427326	13.405763840615784
32-33	15.587850145165827	52.264118553116035	18.79579811109462	13.352233190623517
34-35	15.691790604694225	52.28574183713237	18.681115015302804	13.341352542870599
36-37	16.237728441292003	52.08641763042464	18.338686249933108	13.337167678350244
38-39	16.59459944124045	52.25353618825979	18.258519382467146	12.893344988032624
40-41	16.86048010290139	52.422140021769316	18.210188250829972	12.507191624499326
42-43	17.037042874083934	52.45124731484094	18.42816795789811	12.08354185317701
44-45	17.166962857183815	52.11974555663026	18.49676612310609	12.216525463079828
46-47	17.17359523882346	51.726516831232416	18.832533020554013	12.267354909390116
48-49	17.090303378232335	51.82317876563843	19.17827502977821	11.908242826351028
50-51	17.344253456798725	51.76597076960782	19.362774155965024	11.527001617628432
52-53	17.496705643339027	51.85389729104358	19.43442711005219	11.214969955565197
54-55	17.469514482193066	52.07248542476046	19.355659885330244	11.102340207716223
56-57	17.33366586685291	52.1565321110916	19.049366981309145	11.460435040746338
58-59	17.077561799661158	51.95770882198497	19.20958583414267	11.755143544211208
60-61	17.04675624473052	51.56634581814947	19.611290194622022	11.775607742497984
62-63	17.056086187567264	50.97266953534728	19.92564830018341	12.045595976902037
64-65	16.777849148103062	50.87187746417574	20.030957348523756	12.319316039197444
66-67	16.26532564397377	50.717371516217135	20.414763477756207	12.602539362052886
68-69	16.0664928963039	50.71295176866282	20.161907570479126	13.058647764554157
70-71	15.711427643881756	50.8246924054039	20.2778435192907	13.186036431423648
72-73	15.758530860524903	51.154662479884735	20.213289209832762	12.873517449757593
74-75	15.590560533858222	51.61352923782151	20.02759898696866	12.76831124135161
76-77	14.975960497153116	52.33612555417429	20.01775387839079	12.670160070281817
78-79	14.431470818155848	53.086800153309696	19.778491614378826	12.703237414155627
80-81	13.865197257456993	54.210635156345475	19.396019034233262	12.528148551964271
82-83	13.338543706026352	55.21456725821685	19.341747840370267	12.105141195386528
84-85	12.803554701551809	56.31394491557117	19.25334082466369	11.629159558213335
86-87	12.40578945418877	57.286756760222154	18.95949280436404	11.347960981225027
88-89	12.140395773007512	58.08208852892256	18.742293431920416	11.035222266149512
90-91	12.1229441167315	58.77642400791545	18.323347902926052	10.777283972426998
92-93	12.196587880410865	59.30701574545314	17.817033262166817	10.679363111969176
94-95	12.417183833402778	59.396931133322504	17.441401336242173	10.744483697032546
96-97	12.684376314334234	59.16597967532575	17.303268749098176	10.846375261241842
98-99	13.03382250831779	58.71821871850721	17.188720143790984	11.059238629384014
100	13.723318643350035	57.81088974926234	17.147212010704152	11.318579596683472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1319309.0
1	1299196.5
2	806299.5
3	207568.5
4	79394.5
5	74093.0
6	62169.5
7	43776.5
8	28410.5
9	20716.5
10	19411.5
11	21820.5
12	26807.0
13	34478.0
14	44909.0
15	58483.0
16	74348.5
17	90706.5
18	107887.0
19	125142.0
20	141366.0
21	155501.5
22	166296.5
23	175122.0
24	181949.5
25	185390.5
26	184751.0
27	180553.0
28	173827.5
29	163906.5
30	151795.0
31	139731.0
32	128066.0
33	117134.0
34	106798.5
35	98165.0
36	91370.0
37	86245.5
38	83407.0
39	82668.5
40	84446.0
41	89706.0
42	100128.0
43	117315.5
44	142489.0
45	174988.5
46	212254.5
47	247863.5
48	276608.0
49	297004.0
50	309623.0
51	319410.0
52	327799.5
53	329287.5
54	317812.0
55	289209.0
56	243866.5
57	188499.0
58	134075.0
59	89273.0
60	57071.0
61	36597.5
62	24478.0
63	17564.0
64	13594.5
65	11083.0
66	9242.5
67	7822.5
68	6785.0
69	5950.0
70	5254.0
71	4647.5
72	4166.0
73	3721.5
74	3437.5
75	3281.0
76	3031.0
77	2811.5
78	2636.0
79	2480.0
80	2295.0
81	2143.0
82	2080.5
83	1981.0
84	1858.5
85	1821.0
86	1782.0
87	1751.5
88	1741.0
89	1718.5
90	1667.5
91	1607.5
92	1561.0
93	1528.0
94	1480.0
95	1415.0
96	1371.5
97	1287.0
98	1152.5
99	950.5
100	769.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.362943861645174E-4
2	4.719102544228453E-4
3	1.7807934129163974E-4
4	4.4519835322909935E-5
5	2.582150448728776E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.787578591978291E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.3423802387491926E-5
22-23	8.903967064581986E-6
24-25	1.4246347303331177E-4
26-27	2.493110778082956E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.2259917661454967E-5
42-43	3.116388472603695E-5
44-45	1.7807934129163972E-5
46-47	2.4485909427600464E-4
48-49	1.3355950596872981E-5
50-51	1.3355950596872981E-5
52-53	4.451983532290993E-6
54-55	1.3355950596872981E-5
56-57	8.903967064581986E-6
58-59	1.3355950596872981E-5
60-61	0.0
62-63	1.7807934129163972E-5
64-65	1.1575157183956582E-4
66-67	1.6027140716247574E-4
68-69	3.1163884726036954E-4
70-71	8.458768711352888E-4
72-73	4.273904190999354E-4
74-75	9.082046405873627E-4
76-77	0.0014557986150591548
78-79	7.790971181509238E-4
80-81	0.0011708716689925313
82-83	0.0013044311749612612
84-85	0.0016828497752059955
86-87	0.002252703667339243
88-89	0.0023951671403725544
90-91	0.002252703667339243
92-93	0.00219482788141946
94-95	0.0011174478666050394
96-97	0.0019188049024174182
98-99	0.0018075053141101433
100	0.0014869624997851918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1230949E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.26018144547314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.24603684972743	66.66017975619815
2	3.1849126322711467	4.411752535981582
3	0.285227456545539	0.592647161807245
4	0.0807802808048136	0.2237942762303064
5	0.048001329922012816	0.1662290410011313
6	0.028903633515504075	0.12011225410303625
7	0.017668802009653033	0.08566211031788966
8	0.014721024007911039	0.08156646350808677
9	0.010308304821160985	0.06425595560779697
>10	0.06941317969669908	0.8833020591401768
>50	0.00661182252473449	0.3222253271574329
>100	0.004428204569016149	0.6408958440495303
>500	9.262305905902613E-4	0.44893912631681615
>1k	0.0010953224114879498	1.5970080499056387
>5k	3.129491618084656E-4	1.5289304931688732
>10k+	6.519774204343033E-4	22.172499545506206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	587374	5.229958750591781	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	167500	1.491414483317483	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143772	1.2801411528090814	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	128740	1.1462967198942848	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93678	0.8341058266759114	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77068	0.6862109337332046	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72948	0.6495265894271267	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68996	0.6143381115878987	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64151	0.571198391159999	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62112	0.5530432023153163	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57682	0.5135986282192182	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52847	0.47054794746196427	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51493	0.45849197605652026	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49650	0.4420819647564957	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48946	0.43581357194302994	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44433	0.3956299685805714	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41598	0.37038722195248147	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39465	0.3513950602037281	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37553	0.33437067517624736	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34642	0.30845122705124917	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32467	0.28908509868578336	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30825	0.27446478476573977	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30655	0.2729511103647608	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26363	0.2347352837235749	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23388	0.20824598170644354	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23229	0.20683025094317495	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23031	0.20506726546438772	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22491	0.20025912324951345	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20565	0.18311008268312856	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19524	0.1738410529688987	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19359	0.17237189840324268	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17823	0.15869540499204476	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16841	0.14995170933462523	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16688	0.14858940237374418	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16123	0.14355866098225536	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15589	0.13880394256976858	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15373	0.1368806856838189	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14461	0.1287602677209201	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13992	0.12458430716763115	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13481	0.12003437999762977	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12658	0.11270641510347879	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12490	0.111210548636629	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12471	0.11104137326240196	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12311	0.10961673853206884	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12262	0.10918044414590432	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11851	0.10552091368236113	No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11564	0.1029654751348261	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002430783008630882	0.0	0.0	0.0	0.0
2	0.00555607544829916	0.0	0.0	8.903967064581988E-6	0.0
3	0.009055334504679881	0.0	0.0	1.7807934129163975E-5	0.0
4	0.01519907177924145	0.0	0.0	1.7807934129163975E-5	0.0
5	0.024129750745017185	0.0	0.0	4.4519835322909935E-5	0.0
6	0.04074455328752717	0.0	0.0	4.4519835322909935E-5	0.0
7	0.06126819737138865	0.0	0.0	4.4519835322909935E-5	0.0
8	0.1294102573166346	0.0	0.0	4.4519835322909935E-5	0.0
9	0.38009254605287585	0.0	0.0	4.4519835322909935E-5	0.0
10-11	0.9221259930928365	0.0	0.0	4.4519835322909935E-5	0.0
12-13	1.6226589578494213	0.0	0.0	4.4519835322909935E-5	0.0
14-15	2.437006881609025	0.0	0.0	6.23277694520739E-5	0.0
16-17	3.307423085974302	0.0	0.0	6.23277694520739E-5	0.0
18-19	4.260441392797706	0.0	0.0	6.67797529843649E-5	0.0
20-21	5.503306087490914	0.0	0.0	7.12317365166559E-5	0.0
22-23	6.900093660829553	0.0	0.0	7.12317365166559E-5	0.0
24-25	8.348421847521523	0.0	0.0	7.56837200489469E-5	0.0
26-27	9.831395370061783	0.0	0.0	8.013570358123789E-5	0.0
28-29	11.469600654405962	0.0	8.903967064581988E-6	8.013570358123789E-5	0.0
30-31	13.277292951824464	0.0	8.903967064581988E-6	9.349165417811087E-5	0.0
32-33	15.286143673165999	4.451983532290994E-6	8.903967064581988E-6	9.794363771040185E-5	0.0
34-35	17.10610563719949	8.903967064581988E-6	8.903967064581988E-6	1.0684760477498384E-4	0.0
36-37	18.52628838400032	8.903967064581988E-6	8.903967064581988E-6	1.1129958830727484E-4	0.0
38-39	19.58252147703636	8.903967064581988E-6	8.903967064581988E-6	1.6472339069476677E-4	0.0
40-41	20.492729510213252	8.903967064581988E-6	8.903967064581988E-6	1.825313248239307E-4	0.0
42-43	21.23361525370652	8.903967064581988E-6	8.903967064581988E-6	2.1369520954996768E-4	0.0
44-45	21.83899152244392	8.903967064581988E-6	8.903967064581988E-6	2.3150314367913164E-4	0.0
46-47	22.361026659456826	8.903967064581988E-6	8.903967064581988E-6	2.5376306134058664E-4	0.0
48-49	22.836770071700975	8.903967064581988E-6	8.903967064581988E-6	2.849269460666236E-4	0.0
50-51	23.310937481774694	8.903967064581988E-6	8.903967064581988E-6	2.893789295989146E-4	0.0
52-53	23.807596312653544	8.903967064581988E-6	8.903967064581988E-6	3.1163884726036954E-4	0.0
54-55	24.31458819731084	8.903967064581988E-6	8.903967064581988E-6	3.6951463318015245E-4	0.0
56-57	24.84192564671071	8.903967064581988E-6	8.903967064581988E-6	4.140344685030624E-4	0.0
58-59	25.3859624863402	1.3355950596872981E-5	8.903967064581988E-6	4.585543038259723E-4	0.0
60-61	25.94130291215818	1.7807934129163975E-5	8.903967064581988E-6	4.897181885520093E-4	0.0
62-63	26.477548780606163	1.7807934129163975E-5	8.903967064581988E-6	5.386900074072101E-4	0.0
64-65	26.986851244716718	1.7807934129163975E-5	8.903967064581988E-6	6.14373727456157E-4	0.0
66-67	27.454158148167174	1.7807934129163975E-5	8.903967064581988E-6	6.94509431037395E-4	0.0
68-69	27.869777522807734	1.7807934129163975E-5	1.7807934129163975E-5	7.390292663603049E-4	0.0
70-71	28.254192944870468	1.7807934129163975E-5	1.7807934129163975E-5	7.790971181509238E-4	0.0
72-73	28.61741247333596	1.7807934129163975E-5	1.7807934129163975E-5	8.013570358123788E-4	0.0
74-75	28.964916499932464	1.7807934129163975E-5	1.7807934129163975E-5	8.280689370061248E-4	0.0
76-77	29.304389148236716	1.7807934129163975E-5	1.7807934129163975E-5	8.547808381998707E-4	0.0
78-79	29.630154139245043	1.7807934129163975E-5	1.7807934129163975E-5	8.814927393936166E-4	0.0
80-81	29.94803911940122	1.7807934129163975E-5	1.7807934129163975E-5	9.927923277008915E-4	4.451983532290994E-6
82-83	30.263969678786715	1.7807934129163975E-5	1.7807934129163975E-5	0.0010551200971529655	8.903967064581988E-6
84-85	30.57775883409318	2.671190119374596E-5	1.7807934129163975E-5	0.0010818319983467114	8.903967064581988E-6
86-87	30.893894184721166	2.671190119374596E-5	1.7807934129163975E-5	0.0010862839818790023	8.903967064581988E-6
88	31.129827052014928	2.671190119374596E-5	1.7807934129163975E-5	0.0010951879489435844	8.903967064581988E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	3785	0.0	48.05484	9
CTGCTGA	3940	0.0	39.48424	9
GCCTGCT	1060	0.0	39.46174	7
GCTTGCT	870	0.0	39.436256	7
TGAAAAA	9770	0.0	39.158302	1
CTTGCTG	2770	0.0	39.02475	8
CCTGCTG	3130	0.0	38.740696	8
GTACGCC	335	0.0	35.074158	3
GTTGCTG	1105	0.0	34.45203	8
ACTGGCC	480	0.0	33.29122	3
ACCGATG	265	0.0	31.924246	1
CATTGCC	460	0.0	30.651766	3
TGCCAAT	510	0.0	30.41136	6
CATAGCC	435	0.0	30.25247	3
CGCCAAT	740	0.0	29.850952	6
GTGCTGA	1310	0.0	29.778229	9
ACCTTGC	680	0.0	29.720325	2
TACCCGA	7315	0.0	29.683815	6
TGCCAAA	1125	0.0	29.661827	6
GCTGAAC	7680	0.0	29.435802	10-11
>>END_MODULE