##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248458_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11230949
Sequences flagged as poor quality	0
Sequence length	100
%GC	29
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31671597832027	34.0	31.0	34.0	30.0	34.0
2	32.58352299525178	34.0	31.0	34.0	31.0	34.0
3	32.705893241969136	34.0	31.0	34.0	31.0	34.0
4	36.19970093355423	37.0	35.0	37.0	35.0	37.0
5	36.08283841374402	37.0	35.0	37.0	35.0	37.0
6	35.99013823319828	37.0	35.0	37.0	35.0	37.0
7	36.01483668031971	37.0	35.0	37.0	35.0	37.0
8	35.86734023990314	37.0	35.0	37.0	35.0	37.0
9	37.56073249019295	39.0	37.0	39.0	35.0	39.0
10-11	37.721445578641664	39.0	37.5	39.0	35.0	39.0
12-13	37.76305635436506	39.0	38.0	39.0	35.0	39.0
14-15	39.23093533769942	41.0	39.0	41.0	36.0	41.0
16-17	39.140584557903345	41.0	39.0	41.0	36.0	41.0
18-19	38.780872791782784	40.0	38.0	41.0	35.0	41.0
20-21	38.28734170193454	40.0	36.5	41.0	34.5	41.0
22-23	37.80513454383953	39.5	35.0	41.0	34.0	41.0
24-25	37.408221736204126	39.0	35.0	41.0	33.5	41.0
26-27	37.21324181954704	39.0	35.0	41.0	33.0	41.0
28-29	37.060820728506556	38.5	35.0	41.0	33.0	41.0
30-31	36.997414287964446	38.0	35.0	41.0	33.0	41.0
32-33	36.92019347608114	38.0	35.0	41.0	33.0	41.0
34-35	36.85911430993053	38.0	35.0	41.0	33.0	41.0
36-37	36.84615307219363	38.0	35.0	41.0	33.0	41.0
38-39	36.741125082127965	38.0	35.0	41.0	33.0	41.0
40-41	36.61254828064841	38.0	35.0	41.0	33.0	41.0
42-43	36.465577485927504	38.0	35.0	40.5	32.5	41.0
44-45	36.30823989139297	37.0	35.0	40.0	32.0	41.0
46-47	36.092135847113184	37.0	35.0	40.0	31.5	41.0
48-49	35.81896894910662	36.0	35.0	40.0	31.0	41.0
50-51	35.5429712128512	35.0	35.0	39.5	31.0	41.0
52-53	35.25679824563356	35.0	35.0	39.0	31.0	41.0
54-55	35.01497927735225	35.0	35.0	38.5	30.5	40.5
56-57	34.73126384956427	35.0	35.0	37.5	30.0	40.0
58-59	34.461040692108924	35.0	34.0	37.0	30.0	40.0
60-61	34.22300034485065	35.0	34.0	36.0	29.5	40.0
62-63	33.98765540650216	35.0	34.0	36.0	29.0	39.0
64-65	33.79708014879242	35.0	34.0	35.0	29.0	39.0
66-67	33.62285484512485	35.0	33.0	35.0	29.0	39.0
68-69	33.45954237705113	35.0	33.0	35.0	29.0	38.0
70-71	33.311442247667586	35.0	33.0	35.0	29.0	37.0
72-73	33.14179990488782	35.0	33.0	35.0	28.0	37.0
74-75	32.99652495973403	35.0	33.0	35.0	27.5	36.0
76-77	32.851549544032295	35.0	33.0	35.0	27.0	36.0
78-79	32.72900989934155	35.0	33.0	35.0	27.0	36.0
80-81	32.63146315596305	35.0	33.0	35.0	27.0	35.0
82-83	32.50954607664944	35.0	33.0	35.0	27.0	35.0
84-85	32.373065891404195	35.0	33.0	35.0	27.0	35.0
86-87	32.2260678950639	35.0	33.0	35.0	26.0	35.0
88-89	32.11064634876358	35.0	32.5	35.0	25.0	35.0
90-91	32.000558189695276	35.0	32.0	35.0	25.0	35.0
92-93	31.84852326370639	35.0	32.0	35.0	25.0	35.0
94-95	31.665755137878374	34.5	32.0	35.0	24.0	35.0
96-97	31.42692995044319	34.0	31.5	35.0	23.5	35.0
98-99	31.182748047382283	34.0	31.0	35.0	21.5	35.0
100	29.96294863417152	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35461558772504986
1101	2	0.32031567732170174
1101	3	0.3225326969830533
1101	4	0.21835514594873473
1101	5	0.17854483175025138
1101	6	0.21594348980419653
1101	7	0.248332252184845
1101	8	0.1786035181272254
1101	9	0.26738503495426613
1101	10-11	0.2682688641792197
1101	12-13	0.2677374149698437
1101	14-15	0.3689780467224395
1101	16-17	0.45020596078970954
1101	18-19	0.4729957840913741
1101	20-21	0.47633139688382187
1101	22-23	0.43757861766857786
1101	24-25	0.41138327391279716
1101	26-27	0.41823670473404917
1101	28-29	0.44262922420411854
1101	30-31	0.4340309298326517
1101	32-33	0.4530205218004397
1101	34-35	0.4985670639736455
1101	36-37	0.516247792250887
1101	38-39	0.5023158946283033
1101	40-41	0.5243403263284634
1101	42-43	0.515688363543056
1101	44-45	0.49782319011947607
1101	46-47	0.508950116036047
1101	48-49	0.5299122814080093
1101	50-51	0.49128969428670644
1101	52-53	0.4825127360514472
1101	54-55	0.4467094065648993
1101	56-57	0.471889177788384
1101	58-59	0.43895525797229595
1101	60-61	0.4506056132495502
1101	62-63	0.45804973288283435
1101	64-65	0.47151545679164997
1101	66-67	0.4422823433459939
1101	68-69	0.44554834086206796
1101	70-71	0.4390950063853225
1101	72-73	0.4332232883421625
1101	74-75	0.4411185835556566
1101	76-77	0.45379806979643433
1101	78-79	0.48453041895380267
1101	80-81	0.4662661764041829
1101	82-83	0.33952709796707126
1101	84-85	0.33843265872625494
1101	86-87	0.4208555161595555
1101	88-89	0.39405291467964076
1101	90-91	0.3330291489978521
1101	92-93	0.34778044519008944
1101	94-95	0.3684116057983715
1101	96-97	0.3339361430011678
1101	98-99	0.336252765873148
1101	100	0.08200314052515623
1102	1	0.27406980250039226
1102	2	0.2778506468531887
1102	3	0.2697215471672578
1102	4	0.16255313323757292
1102	5	0.17552994711444114
1102	6	0.14775469440656508
1102	7	0.19708535332600974
1102	8	0.011618138159391833
1102	9	0.09424636641147544
1102	10-11	0.21075273800214944
1102	12-13	0.2040240225553518
1102	14-15	0.3288361281371053
1102	16-17	0.379405961851937
1102	18-19	0.3936242632028737
1102	20-21	0.2999207143186453
1102	22-23	0.2416697474387206
1102	24-25	0.2192921811472459
1102	26-27	0.2798250591817393
1102	28-29	0.29753122092710527
1102	30-31	0.28536594760374356
1102	32-33	0.30766662580522564
1102	34-35	0.31682706326392207
1102	36-37	0.30099436544937674
1102	38-39	0.3097774924092036
1102	40-41	0.34517914580467846
1102	42-43	0.31479980300914434
1102	44-45	0.3018489543733551
1102	46-47	0.32149589391880795
1102	48-49	0.3512135193876631
1102	50-51	0.3611790223620872
1102	52-53	0.33513964484579617
1102	54-55	0.3100137598553445
1102	56-57	0.30676595350637825
1102	58-59	0.26536852061818905
1102	60-61	0.26829547371369245
1102	62-63	0.2893002303164067
1102	64-65	0.30622124032854003
1102	66-67	0.3127150114272581
1102	68-69	0.3170754213866971
1102	70-71	0.25985945870139915
1102	72-73	0.23268094287799812
1102	74-75	0.2365768678116993
1102	76-77	0.2771170406804231
1102	78-79	0.24596997225773976
1102	80-81	0.24368062318823291
1102	82-83	0.20927004139205962
1102	84-85	0.22727634847148437
1102	86-87	0.27955554053623644
1102	88-89	0.21861954725157773
1102	90-91	0.16382519684530905
1102	92-93	0.20243251774689242
1102	94-95	0.15317191903318772
1102	96-97	0.11100776795987244
1102	98-99	0.16961070456759586
1102	100	0.13797409949065198
1103	1	0.22244482674148713
1103	2	0.2240340249621724
1103	3	0.24712986444902185
1103	4	0.1647820253790684
1103	5	0.1606464360196469
1103	6	0.0822343799668559
1103	7	0.18174124501270938
1103	8	0.16188802481169517
1103	9	0.16687677824924663
1103	10-11	0.19621178062244837
1103	12-13	0.1918407363384631
1103	14-15	0.273942587294421
1103	16-17	0.3254635484996342
1103	18-19	0.36161024499667604
1103	20-21	0.32190077414470863
1103	22-23	0.29985226562286016
1103	24-25	0.28381381336357236
1103	26-27	0.32288162249162866
1103	28-29	0.3171682724364828
1103	30-31	0.31531836266684365
1103	32-33	0.33540737849438074
1103	34-35	0.324595763756399
1103	36-37	0.30829355528875624
1103	38-39	0.31173434012495704
1103	40-41	0.35335414214983984
1103	42-43	0.3173340336347934
1103	44-45	0.3478380191977948
1103	46-47	0.34220374973088497
1103	48-49	0.33574009650829595
1103	50-51	0.30272092023225383
1103	52-53	0.3375447619911469
1103	54-55	0.28519984544880117
1103	56-57	0.2171089240845916
1103	58-59	0.2365433985443559
1103	60-61	0.202397758215497
1103	62-63	0.19231668185741313
1103	64-65	0.1532695630286156
1103	66-67	0.1965935802113421
1103	68-69	0.11616719657155983
1103	70-71	0.12979591379201594
1103	72-73	0.12846704978162649
1103	74-75	0.08477641475971609
1103	76-77	0.11494934394573875
1103	78-79	0.10117511971533588
1103	80-81	0.020544077643485537
1103	82-83	0.053642351582610104
1103	84-85	0.07206329136664635
1103	86-87	0.13460800644038073
1103	88-89	0.06364424739657437
1103	90-91	-0.0052017366321379654
1103	92-93	0.03700592651250645
1103	94-95	0.002932459941106913
1103	96-97	-0.04388789987782005
1103	98-99	-0.09460757580991697
1103	100	-0.12126799132992616
1104	1	0.1664272564566076
1104	2	0.17215711413295764
1104	3	0.19967938093292048
1104	4	0.14407421187848968
1104	5	0.08551717862363262
1104	6	0.06279930019479707
1104	7	0.06902558761270683
1104	8	-0.0029108880557373595
1104	9	0.0027128773793947403
1104	10-11	0.09393303502343286
1104	12-13	0.14074619093828744
1104	14-15	0.21023329870220664
1104	16-17	0.23654827345235674
1104	18-19	0.24333904242324422
1104	20-21	0.2282845010434471
1104	22-23	0.20407412364031075
1104	24-25	0.21660027889949873
1104	26-27	0.21571398841900447
1104	28-29	0.18709716218086925
1104	30-31	0.20180501582844812
1104	32-33	0.19804616667670416
1104	34-35	0.19175321919841792
1104	36-37	0.1420888047212756
1104	38-39	0.17365045487811415
1104	40-41	0.18485868578090958
1104	42-43	0.1929200679805092
1104	44-45	0.18077499952077147
1104	46-47	0.14849620222022963
1104	48-49	0.1732356270055604
1104	50-51	0.15347782351158656
1104	52-53	0.1907376376972394
1104	54-55	0.14723266561470183
1104	56-57	0.1426384745055671
1104	58-59	0.08112662598006182
1104	60-61	0.10599496040022416
1104	62-63	0.07930131860364042
1104	64-65	0.08164072050212212
1104	66-67	0.0830913993431821
1104	68-69	0.07897942326224694
1104	70-71	0.0624678230140816
1104	72-73	0.029880393754559975
1104	74-75	0.029430219848286754
1104	76-77	0.06409856553590032
1104	78-79	0.055940403857349
1104	80-81	0.036341776425786065
1104	82-83	0.029629083855965632
1104	84-85	0.04701099662738528
1104	86-87	0.06789399730578083
1104	88-89	0.049780581868617446
1104	90-91	0.034248165692879695
1104	92-93	0.02125661360121711
1104	94-95	-0.0240379055427411
1104	96-97	-0.217378749700039
1104	98-99	-0.23622587217875335
1104	100	-0.31289264155629937
1105	1	0.21070241097026354
1105	2	0.20469372372309635
1105	3	0.18349094389820664
1105	4	0.13929189076382897
1105	5	0.10828364847606053
1105	6	0.09190210935005183
1105	7	0.1318067054667864
1105	8	0.0010543227171950775
1105	9	0.12003175155118839
1105	10-11	0.17166486650267387
1105	12-13	0.17247225099181662
1105	14-15	0.25679228286939093
1105	16-17	0.2614845473554013
1105	18-19	0.25463625790448674
1105	20-21	0.19781064726520015
1105	22-23	0.17581762859200012
1105	24-25	0.1533752429861579
1105	26-27	0.16235215599952824
1105	28-29	0.19490390287999304
1105	30-31	0.17120797958661882
1105	32-33	0.17817570464129773
1105	34-35	0.15684825565923433
1105	36-37	0.18840111898056477
1105	38-39	0.18961437402043657
1105	40-41	0.18800381079027773
1105	42-43	0.21013022767734668
1105	44-45	0.18906918870047207
1105	46-47	0.1887696896050315
1105	48-49	0.21301144533583027
1105	50-51	0.18539187815461844
1105	52-53	0.17684583950700983
1105	54-55	0.15429473261644233
1105	56-57	0.18621319984437434
1105	58-59	0.17648984018543956
1105	60-61	0.17468958780004584
1105	62-63	0.15857650138379853
1105	64-65	0.17474345467674368
1105	66-67	0.1884898931251442
1105	68-69	0.19089559343018436
1105	70-71	0.18504606768183152
1105	72-73	0.17015931128419481
1105	74-75	0.18328393412685529
1105	76-77	0.18189903404581997
1105	78-79	0.12929968931574365
1105	80-81	0.10692905477174008
1105	82-83	0.1272610125720064
1105	84-85	0.1389314766938483
1105	86-87	0.17096199938142576
1105	88-89	0.10353231356412351
1105	90-91	0.09420689510444191
1105	92-93	0.0936295095316737
1105	94-95	0.09832809484763061
1105	96-97	0.10716409905213453
1105	98-99	0.09001083214882044
1105	100	0.05791113592023578
1106	1	0.09306267548652158
1106	2	0.11802008149917498
1106	3	0.10148529824253671
1106	4	0.06976009611289413
1106	5	0.0383647277923842
1106	6	0.06954974822268412
1106	7	0.09240948493064138
1106	8	0.03866987362390972
1106	9	0.08550456888764302
1106	10-11	0.10030533769623418
1106	12-13	0.13094244092875584
1106	14-15	0.17739920062908965
1106	16-17	0.15805559417231763
1106	18-19	0.16067205713942911
1106	20-21	0.11942226910540654
1106	22-23	0.11156025631987632
1106	24-25	0.12489730980976077
1106	26-27	0.12202240644822382
1106	28-29	0.13609661420040453
1106	30-31	0.13918374638971898
1106	32-33	0.1505175453597758
1106	34-35	0.13810586257759638
1106	36-37	0.09356853882398042
1106	38-39	0.1198578885650079
1106	40-41	0.05310345263347216
1106	42-43	0.09584600737348836
1106	44-45	0.10573047152848147
1106	46-47	0.09952081502857624
1106	48-49	0.1055273834390178
1106	50-51	0.11895332882901499
1106	52-53	0.13759115412257472
1106	54-55	0.13204529337665605
1106	56-57	0.1014311646602124
1106	58-59	0.11791063506314003
1106	60-61	0.11549474539960158
1106	62-63	0.07393622863752825
1106	64-65	0.08835762139782588
1106	66-67	0.11051082686994107
1106	68-69	0.08285149730452446
1106	70-71	0.09645874755487682
1106	72-73	0.09479017043530291
1106	74-75	0.07691295751117622
1106	76-77	0.04466314295927987
1106	78-79	0.06666396303073441
1106	80-81	0.05453071566223855
1106	82-83	0.020023586552341044
1106	84-85	0.04205663186557018
1106	86-87	0.024641081826963784
1106	88-89	0.009875480388913616
1106	90-91	-0.02191904367816022
1106	92-93	0.07314009674616528
1106	94-95	0.03904339122025746
1106	96-97	0.019695708079233043
1106	98-99	0.020611645972458348
1106	100	0.009546352199741648
1107	1	0.14751417164452363
1107	2	0.122003372816053
1107	3	0.1334870856334831
1107	4	0.08614015119185581
1107	5	0.11082371783655987
1107	6	0.0813624121483798
1107	7	0.08685881837926246
1107	8	0.0874192294219327
1107	9	0.08194872824342525
1107	10-11	0.10475100632020684
1107	12-13	0.10844483908874736
1107	14-15	0.16334644244181362
1107	16-17	0.13905862568216776
1107	18-19	0.18614592997618473
1107	20-21	0.14800874637931827
1107	22-23	0.1536402588949244
1107	24-25	0.17927637203401048
1107	26-27	0.15307539626419953
1107	28-29	0.16957672986188044
1107	30-31	0.205562370882717
1107	32-33	0.17912520792886255
1107	34-35	0.19035570445928585
1107	36-37	0.17707050598487228
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2216	34-35	-0.4169691647394842
2216	36-37	-0.48600773953906895
2216	38-39	-0.45497042137206734
2216	40-41	-0.4423748565388408
2216	42-43	-0.3776678929091801
2216	44-45	-0.41529064460522136
2216	46-47	-0.45504817007415
2216	48-49	-0.5089809351678909
2216	50-51	-0.5267216465585705
2216	52-53	-0.4556610785290687
2216	54-55	-0.38428976310600405
2216	56-57	-0.3563874995204088
2216	58-59	-0.34898514443343487
2216	60-61	-0.383983954363174
2216	62-63	-0.3842352167001408
2216	64-65	-0.32315477136233994
2216	66-67	-0.2800346269293925
2216	68-69	-0.27740492514796955
2216	70-71	-0.2372336249632241
2216	72-73	-0.2598727410416828
2216	74-75	-0.27494823215948117
2216	76-77	-0.32619196290117713
2216	78-79	-0.20350410278543052
2216	80-81	-0.2580799656252566
2216	82-83	-0.2932372021568739
2216	84-85	-0.27065581931528726
2216	86-87	-0.29710637588425115
2216	88-89	-0.35486843290970427
2216	90-91	-0.3129631718201864
2216	92-93	-0.30540594797513876
2216	94-95	-0.3540153275460298
2216	96-97	-0.3106417367130234
2216	98-99	-0.329635336277871
2216	100	-0.16090540465526715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	72.0
8	313.0
9	821.0
10	1523.0
11	2445.0
12	3654.0
13	5306.0
14	6888.0
15	9021.0
16	12120.0
17	16458.0
18	22075.0
19	27739.0
20	35160.0
21	43889.0
22	54193.0
23	65644.0
24	77691.0
25	92312.0
26	108786.0
27	129244.0
28	155496.0
29	193149.0
30	220214.0
31	271451.0
32	351685.0
33	503384.0
34	734695.0
35	3114789.0
36	1717345.0
37	2257215.0
38	861979.0
39	133951.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.23159241217711	22.495994840615367	33.05198712469864	26.220425622508888
2	15.264702084937959	22.665434907462235	40.69858555458103	21.371277453018774
3	11.780874439016685	19.624922168197898	45.669257335243884	22.924946057541533
4	11.924495427768393	16.36511749808498	50.48628570924861	21.224101364898015
5	11.239673012799061	17.247406554668274	52.714353160923174	18.798567271609492
6	10.373175054040402	13.10297998860114	49.12429929118189	27.39954566617656
7	14.356195545006928	13.809972781463081	47.02040762539301	24.813424048136984
8	10.482034955371981	13.524004071249902	48.28491341203669	27.709047561341432
9	10.682133807214331	12.332519718502862	50.98454280221556	26.000803672067246
10-11	13.86450067576658	19.135422126838968	51.39772694186395	15.602350255530498
12-13	12.368100474541567	25.097835716310414	47.15951984269889	15.374543966449124
14-15	13.806310194777158	23.049189929566875	46.97860917570814	16.165890699947834
16-17	18.636092105840742	23.379475768254313	47.29166253003197	10.692769595872974
18-19	18.60425597160133	18.87344960786484	46.992542660464395	15.529751760069429
20-21	13.982907931125979	18.18177285249498	51.67864844794826	16.156670768430782
22-23	13.906034468328052	19.271997341631355	52.9130265762071	13.90894161383349
24-25	14.05758037176379	20.619230563410166	51.096244706874586	14.226944357951465
26-27	17.367382555116286	20.53891975638423	47.843181251558214	14.250516436941268
28-29	15.640992262187043	22.94040899232487	50.09555400308188	11.32304474240621
30-31	15.682252669447433	20.998277465643582	50.261710270798645	13.05775959411034
32-33	16.28376977490089	21.745451362651227	49.903953931722725	12.066824930725156
34-35	16.252096306720375	21.759575829372597	50.04623683866195	11.942091025245077
36-37	16.534478321095	21.011863137324582	49.75937012257417	12.694288419006256
38-39	16.991249855194184	21.182689335889574	49.05936989590098	12.766690913015266
40-41	17.022313095684634	21.54229827000326	49.20227816851635	12.23311046579575
42-43	17.028832375711982	21.804698670386294	49.25948871011509	11.906980243786629
44-45	17.281638042255736	21.06119204123333	49.10529812428536	12.551871792225574
46-47	17.499650388459493	20.68580138155439	49.42399022147318	12.390558008512935
48-49	16.847460058501717	20.71303994201029	49.64633748516342	12.79316251432457
50-51	16.740333432515403	20.62228519138392	49.63196744204249	13.005413934058186
52-53	16.5438024686521	20.930694352090686	49.555375931053874	12.970127248203342
54-55	16.395443565883593	21.10766519437799	49.743316354656805	12.753574885081612
56-57	16.27058154918168	21.083983294566057	49.61185755458751	13.033577601664756
58-59	16.058274861091324	21.11698603789714	50.11151428266135	12.713224818350191
60-61	15.64821483206161	20.66000957398952	50.42084326616938	13.270932327779494
62-63	15.532745127938568	20.46144034263873	50.521943059016806	13.483871470405893
64-65	15.540677142609225	20.421222805910837	50.604565379632604	13.433534671847339
66-67	15.155272562303356	20.135142535194714	51.28940358756573	13.4201813149362
68-69	15.061196358274312	19.721832185304763	51.90191468985509	13.315056766565833
70-71	14.72564641463281	19.46357771685048	52.423925764624514	13.3868501038922
72-73	14.536196367908683	19.153626515161466	52.9178738132863	13.392303303643555
74-75	14.541894456804524	18.838440475892085	53.19189639121299	13.427768676090404
76-77	14.627589609157155	18.68639823479384	53.62866160986022	13.057350546188786
78-79	14.625737358967486	18.62321698578023	54.10342193683636	12.647623718415923
80-81	14.370580454165655	18.366336051811842	54.473811820217136	12.789271673805375
82-83	14.322210548571126	18.371446871712056	54.3300932681637	12.976249311553117
84-85	14.220715831302202	18.560275648702834	54.0568933701303	13.162115149864661
86-87	14.335783061592691	18.762038004734734	53.724443969561484	13.17773496411109
88-89	14.267860508089433	19.29259735837231	53.45838427344732	12.981157860090937
90-91	14.271977342559822	19.753489572474646	53.16330814974315	12.811224935222384
92-93	14.086971310072304	20.10187922743812	52.79308197128558	13.018067491203992
94-95	13.996326564629168	20.704882705791093	52.51278525753581	12.786005472043923
96-97	13.560529958459316	21.52942884989455	52.62315155359517	12.286889638050958
98-99	13.321636900464878	22.325379251448776	52.41839732424509	11.934586523841254
100	13.101482569787631	23.186590089488448	51.950794710832106	11.761132629891813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	430442.0
1	887698.0
2	1173214.0
3	655867.5
4	189417.5
5	50550.0
6	26598.5
7	18505.0
8	16031.0
9	16700.0
10	19459.5
11	23891.5
12	30728.5
13	40784.0
14	53651.5
15	68397.0
16	85652.5
17	105436.0
18	125343.0
19	141912.0
20	153767.0
21	162624.0
22	169641.5
23	175422.0
24	179841.0
25	181547.5
26	180020.0
27	174981.0
28	167340.0
29	157756.0
30	145971.0
31	133614.0
32	121761.0
33	109885.5
34	98103.0
35	87548.5
36	77925.5
37	68451.0
38	60264.0
39	53363.0
40	47437.0
41	43490.0
42	42648.5
43	46752.0
44	58034.0
45	77721.5
46	104562.0
47	137574.5
48	178363.5
49	231899.5
50	302291.0
51	385553.5
52	465959.5
53	518767.0
54	521890.5
55	468362.5
56	371997.5
57	261904.0
58	165124.0
59	94432.5
60	49983.0
61	24797.0
62	11749.0
63	5675.5
64	2890.5
65	1683.5
66	1127.0
67	825.0
68	628.5
69	463.5
70	357.0
71	280.5
72	212.5
73	156.0
74	121.5
75	93.0
76	62.5
77	41.0
78	25.5
79	19.0
80	19.5
81	17.0
82	11.0
83	9.5
84	8.0
85	8.5
86	7.0
87	6.0
88	7.0
89	4.5
90	3.5
91	2.0
92	1.0
93	1.0
94	1.5
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042774657778251866
2	0.003552682858768213
3	0.0
4	0.0
5	0.008075898127575863
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.0067851790618936E-5
14-15	1.0684760477498385E-4
16-17	0.0
18-19	0.0
20-21	8.013570358123787E-5
22-23	8.903967064581986E-6
24-25	9.616284429748545E-4
26-27	0.001326691092622716
28-29	0.0011308038172019124
30-31	0.0010106002618300554
32-33	0.0012510073725737693
34-35	0.0016516858904799584
36-37	0.002844817477133945
38-39	0.0034903550893161392
40-41	0.003886581623690037
42-43	0.003842061788367127
44-45	0.005484843711782504
46-47	0.005587239333025197
48-49	0.006059149587448041
50-51	0.006771466952614601
52-53	0.006513251907741724
54-55	0.005235532633974208
56-57	0.004198220470950407
58-59	0.00488827791845551
60-61	0.0046389668406472155
62-63	0.004109180800304587
64-65	0.0032098801267818064
66-67	0.0019054489518205452
68-69	0.001553742252769557
70-71	6.85605463972813E-4
72-73	8.147129864092518E-4
74-75	0.0011174478666050394
76-77	7.078653816342679E-4
78-79	6.455376121821941E-4
80-81	0.001397922829139372
82-83	4.763622379551363E-4
84-85	0.0011797756360571133
86-87	9.927923277008915E-4
88-89	2.893789295989146E-4
90-91	2.181471930822587E-4
92-93	3.5615868258327944E-5
94-95	4.0067851790618947E-4
96-97	2.7602297900204155E-4
98-99	0.001553742252769557
100	0.003997881211997312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1230949E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.59463136989162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.26680042432841	64.39524256471512
2	4.13022937223703	5.583626637789218
3	0.34086798353262726	0.6912253707805867
4	0.07887617336185831	0.2132642344904991
5	0.0331842378567696	0.11215381626095715
6	0.02133232562018924	0.08651704119355116
7	0.017277969672179448	0.08175285935677958
8	0.012112817968515623	0.06550091723459303
9	0.009825460449260822	0.05977335393965116
>10	0.0669911847318179	0.8947247587218328
>50	0.010008157226080413	0.466908534765025
>100	0.008783576655673418	1.2064074479660885
>500	9.380143197354848E-4	0.4275886058935977
>1k	0.0013124909844785568	2.1163429186740794
>5k	3.883900973111098E-4	1.9031676397591462
>10k+	0.0010714209580996131	21.69580329845927
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81370	0.7245158000450362	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69002	0.6143915353902862	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67857	0.6041964931013398	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67530	0.6012848958712216	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63134	0.5621430566553192	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62381	0.5554383694556889	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56947	0.5070542124267504	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56252	0.5008659553168658	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56068	0.49922762537698284	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52939	0.47136711243190577	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51606	0.45949812433481796	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51382	0.4575036357123517	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51047	0.4545208067457167	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48704	0.43365881191340105	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48171	0.4289129974679789	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46710	0.4159043015866246	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44842	0.39927169110998545	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44837	0.3992271712746625	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43796	0.3899581415604327	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43555	0.38781228549786845	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43119	0.3839301558577107	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42747	0.38061788010968617	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42035	0.3742782555597038	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41481	0.36934545780592537	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40869	0.36389622996240123	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40806	0.36333528003733256	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39961	0.35581142786776077	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38701	0.3445924293663875	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38229	0.34038975691190476	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38119	0.33941032053480075	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36870	0.3282892656711378	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33802	0.3009718947170003	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33224	0.29582540175367195	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32045	0.28532762458452976	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29361	0.2614293769831917	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25981	0.2313339683049046	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25245	0.22478064854537225	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24401	0.21726570034286508	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21640	0.1926818472775542	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21205	0.18880862160446105	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21176	0.18855040655958816	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21033	0.1872771392693529	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20898	0.18607510371563438	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20877	0.18588812040727815	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19815	0.17643210738469206	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19691	0.1753280154686839	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19062	0.16972742018506184	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18734	0.16680691898787894	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18437	0.1641624407696981	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17614	0.1568344758755471	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17412	0.15503587452850154	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16620	0.1479839326133526	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16065	0.14304223089250961	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15712	0.13989913051871217	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15506	0.13806491330340828	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15490	0.13792244983037497	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15009	0.13363964167231104	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15000	0.1335595059687298	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14951	0.1331232115825653	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14898	0.13265130132814246	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14777	0.131573921313328	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14686	0.13076366031045106	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14226	0.12666783546074334	No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14178	0.12624044504164342	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14062	0.12520758486215192	No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13722	0.12218023606019401	No Hit
ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13379	0.11912617535704241	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13247	0.11795085170451759	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12962	0.11541322109111171	No Hit
GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12858	0.1144872085163952	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11377	0.10130043329374926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002065720358983021	0.0	0.0	0.0	0.0
2	0.004719102544228453	0.0	0.0	0.0	0.0
3	0.007630699774346762	0.0	0.0	0.0	0.0
4	0.013008695881354282	0.0	0.0	0.0	0.0
5	0.021609928065740483	0.0	0.0	0.0	0.0
6	0.0395425177338086	0.0	0.0	0.0	0.0
7	0.060484648269705434	0.0	0.0	0.0	0.0
8	0.1568878996779346	0.0	0.0	0.0	0.0
9	0.5398475231256059	0.0	0.0	0.0	0.0
10-11	1.2827099473072132	0.0	0.0	8.903967064581988E-6	0.0
12-13	2.0517767465598857	0.0	0.0	1.7807934129163975E-5	0.0
14-15	2.8685643572951847	0.0	0.0	3.561586825832795E-5	0.0
16-17	3.673398392246283	0.0	0.0	4.4519835322909935E-5	0.0
18-19	4.536931830070638	0.0	0.0	6.23277694520739E-5	0.0
20-21	5.658769352438516	0.0	0.0	6.23277694520739E-5	0.0
22-23	6.976921540646298	0.0	0.0	6.23277694520739E-5	0.0
24-25	8.46133750585102	0.0	0.0	6.23277694520739E-5	0.0
26-27	10.048055600644254	0.0	0.0	6.23277694520739E-5	0.0
28-29	11.838006743686575	0.0	0.0	6.23277694520739E-5	0.0
30-31	13.78746800470735	0.0	0.0	7.12317365166559E-5	0.0
32-33	15.975666882647227	0.0	0.0	7.12317365166559E-5	0.0
34-35	18.008224416298212	0.0	0.0	7.12317365166559E-5	0.0
36-37	19.52363063887121	0.0	0.0	7.56837200489469E-5	0.0
38-39	20.61966891666946	0.0	0.0	9.349165417811087E-5	0.0
40-41	21.651563015734467	0.0	0.0	1.1129958830727484E-4	0.0
42-43	22.48866947931114	0.0	0.0	1.246555389041478E-4	0.0
44-45	23.14864932607209	0.0	0.0	1.246555389041478E-4	0.0
46-47	23.70123842606711	0.0	0.0	1.335595059687298E-4	0.0
48-49	24.19124599354872	0.0	0.0	1.6027140716247577E-4	0.0
50-51	24.672060215036147	0.0	0.0	1.6027140716247577E-4	0.0
52-53	25.182355471474406	0.0	0.0	1.6472339069476677E-4	0.0
54-55	25.670657929263143	0.0	0.0	2.1369520954996768E-4	0.0
56-57	26.158595324402242	0.0	0.0	2.1814719308225867E-4	0.0
58-59	26.622914946902526	0.0	0.0	2.3150314367913164E-4	0.0
60-61	27.072498503910936	0.0	0.0	2.4040711074371364E-4	0.0
62-63	27.51763898135411	0.0	0.0	2.4040711074371364E-4	0.0
64-65	27.95366179652316	0.0	0.0	2.493110778082956E-4	0.0
66-67	28.359905293844715	0.0	0.0	3.1163884726036954E-4	0.0
68-69	28.72412206662144	0.0	0.0	3.338987649218245E-4	0.0
70-71	29.06956927682603	0.0	0.0	3.428027319864065E-4	0.0
72-73	29.409794310347237	0.0	4.451983532290994E-6	3.561586825832795E-4	0.0
74-75	29.739846561497163	0.0	8.903967064581988E-6	3.784186002447344E-4	0.0
76-77	30.065865315566832	0.0	8.903967064581988E-6	4.0958248497077136E-4	0.0
78-79	30.37299430350899	0.0	8.903967064581988E-6	4.8526620501971826E-4	0.0
80-81	30.673298400696147	0.0	8.903967064581988E-6	6.099217439238661E-4	0.0
82-83	30.971603557277305	0.0	8.903967064581988E-6	6.49989595714485E-4	0.0
84-85	31.27059877130597	0.0	8.903967064581988E-6	6.76701496908231E-4	0.0
86-87	31.567011834885903	0.0	8.903967064581988E-6	6.76701496908231E-4	0.0
88	31.790786335152976	0.0	8.903967064581988E-6	6.76701496908231E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	2895	0.0	62.212406	1
GACTTTT	2750	0.0	56.942738	1
TGCCCTA	6380	0.0	53.406326	6
GCATTTT	2125	0.0	53.331642	1
CACTTTT	4515	0.0	50.826344	1
GCCCTAC	16485	0.0	50.40443	7
CCCCTAC	40960	0.0	48.74162	7
GCCCCTA	4810	0.0	48.267773	6
TGCTCTA	6025	0.0	46.25653	6
CGCCCTA	6610	0.0	46.21546	6
CCCCCTA	21325	0.0	46.171116	6
CCCTTTT	6420	0.0	44.973858	1
TCGCCCT	2205	0.0	44.549824	5
CCGCCTA	3070	0.0	43.629665	6
AGCTTTT	1785	0.0	43.204845	1
TTGCTCT	2255	0.0	43.145157	5
ATGCCCT	2225	0.0	42.670685	5
CTCCCTA	15350	0.0	42.649906	6
GTCCCTA	1885	0.0	42.6344	6
ACTTTGC	460	0.0	41.894173	2
>>END_MODULE