##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248457_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20121114
Sequences flagged as poor quality	0
Sequence length	100
%GC	29
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.111242946091355	31.0	28.0	33.0	22.0	34.0
2	29.647936441292465	31.0	30.0	33.0	24.0	34.0
3	29.960370186263045	31.0	30.0	34.0	25.0	34.0
4	33.360752043848066	35.0	33.0	37.0	28.0	37.0
5	33.18879029262495	35.0	33.0	37.0	27.0	37.0
6	32.977565108969614	35.0	32.0	37.0	26.0	37.0
7	33.1376358684713	35.0	33.0	37.0	27.0	37.0
8	33.04541532839583	35.0	32.0	37.0	27.0	37.0
9	34.28661787811549	37.0	33.0	39.0	27.0	39.0
10-11	34.50672743566783	37.0	34.0	39.0	27.0	39.0
12-13	34.43253937132904	37.0	33.5	39.0	27.0	39.0
14-15	35.137017339099614	38.0	32.5	40.0	27.0	41.0
16-17	34.937070432581415	37.5	32.0	40.0	27.0	41.0
18-19	34.47814676165544	37.0	32.0	40.0	26.5	41.0
20-21	34.1551362663121	36.0	32.0	39.5	26.5	40.5
22-23	33.65976625349869	35.0	32.0	39.0	25.0	40.0
24-25	33.22546771018742	35.0	31.5	38.0	25.0	40.0
26-27	32.78803713353048	35.0	30.0	38.0	24.0	40.0
28-29	32.644563243367145	35.0	30.0	38.0	24.0	40.0
30-31	32.52354613666023	35.0	30.0	38.0	24.0	40.0
32-33	32.339195956048954	35.0	30.0	38.0	23.0	40.0
34-35	32.21003695421635	35.0	30.0	38.0	23.0	40.0
36-37	31.996427011943773	35.0	30.0	37.5	22.0	40.0
38-39	31.812838121189515	34.5	28.5	37.0	22.0	40.0
40-41	31.593341675813775	34.0	28.0	37.0	21.5	40.0
42-43	31.38882573797852	34.0	28.0	37.0	21.0	40.0
44-45	31.166340740378487	33.5	27.0	37.0	20.0	40.0
46-47	31.038252827353396	33.0	27.0	37.0	20.0	39.5
48-49	30.879995859076192	33.0	26.5	36.5	20.0	39.0
50-51	30.70580977772901	33.0	27.0	36.0	20.0	39.0
52-53	30.47215134311152	33.0	27.0	35.5	20.0	39.0
54-55	30.301864300356335	33.0	26.5	35.0	19.5	39.0
56-57	30.12257944565097	33.0	27.0	35.0	18.0	39.0
58-59	29.851443215320984	33.0	26.0	35.0	18.0	39.0
60-61	29.599996476338237	32.5	26.0	35.0	17.0	38.0
62-63	29.54235508530989	32.0	26.0	35.0	17.5	38.0
64-65	29.770787094591284	32.5	26.5	35.0	19.0	38.0
66-67	30.190132564230787	33.0	28.0	35.0	20.0	38.0
68-69	30.174551543219724	33.0	28.0	35.0	20.0	37.5
70-71	30.469662067418334	33.0	28.0	35.0	20.0	37.0
72-73	30.459453562064205	33.0	28.0	35.0	20.0	37.0
74-75	30.361775371880505	33.0	28.0	35.0	20.0	36.5
76-77	30.251290609456316	33.0	28.0	35.0	20.0	36.0
78-79	30.138243215559534	33.0	28.0	35.0	20.0	36.0
80-81	30.0675066251302	33.0	28.0	35.0	20.0	35.5
82-83	29.99775270892059	33.0	28.0	35.0	20.0	35.0
84-85	29.929406517949253	33.0	28.0	35.0	20.0	35.0
86-87	29.80806199398304	33.0	28.0	35.0	19.0	35.0
88-89	29.7063053268323	33.0	28.0	35.0	19.0	35.0
90-91	29.55818994415518	33.0	28.0	35.0	18.0	35.0
92-93	29.408665618613362	33.0	28.0	35.0	17.0	35.0
94-95	29.198607492607017	33.0	27.5	35.0	15.0	35.0
96-97	28.963859481140062	33.0	27.0	35.0	14.0	35.0
98-99	28.681866048768473	33.0	26.5	35.0	10.0	35.0
100	27.373201453955282	31.0	24.0	34.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6648560112502224
1101	2	0.6027242674347235
1101	3	0.5748504716405947
1101	4	0.5186348872959883
1101	5	0.43156091581175815
1101	6	0.3006561403773489
1101	7	0.37115691267906925
1101	8	0.49470468434915205
1101	9	0.476898263740253
1101	10-11	0.5324757020027491
1101	12-13	0.6551681485800174
1101	14-15	0.7820839076429706
1101	16-17	0.7483494122156102
1101	18-19	0.7578449198517916
1101	20-21	0.6734074469051663
1101	22-23	0.6075484379923708
1101	24-25	0.6096247309410927
1101	26-27	0.6811694861807709
1101	28-29	0.5353032665354291
1101	30-31	0.4785644533229032
1101	32-33	0.5004311098066836
1101	34-35	0.42012453831548413
1101	36-37	0.4238548718026607
1101	38-39	0.36390716374295806
1101	40-41	0.3536681989844155
1101	42-43	0.3232672869702746
1101	44-45	0.31631548209469074
1101	46-47	0.33788929247074506
1101	48-49	0.3291696311479626
1101	50-51	0.34485240613731705
1101	52-53	0.3084909867387715
1101	54-55	0.23398726682481197
1101	56-57	0.2729811805875926
1101	58-59	0.28012291520615307
1101	60-61	0.24421550892797583
1101	62-63	0.259241477351253
1101	64-65	0.34176747153605547
1101	66-67	0.33664452885725993
1101	68-69	0.4059689333613363
1101	70-71	0.4070139593831179
1101	72-73	0.3792881516467581
1101	74-75	0.3784660268414832
1101	76-77	0.378190744302902
1101	78-79	0.3523885333906307
1101	80-81	0.3413884864651493
1101	82-83	0.29756910800334424
1101	84-85	0.3149009493197461
1101	86-87	0.29019916601984974
1101	88-89	0.33972647678640655
1101	90-91	0.34693945743484633
1101	92-93	0.3061021775124466
1101	94-95	0.3117475858955814
1101	96-97	0.28993169093977045
1101	98-99	0.2944149250026058
1101	100	0.1698411902377579
1102	1	0.40194650719072555
1102	2	0.38597474303682233
1102	3	0.36272252379033887
1102	4	0.34013342203886054
1102	5	0.31111737256062355
1102	6	0.22647882053595936
1102	7	0.26481719744035814
1102	8	0.34846227457283163
1102	9	0.2946760823365864
1102	10-11	0.28988584165365694
1102	12-13	0.346354871965346
1102	14-15	0.4739944565624228
1102	16-17	0.46898234881783907
1102	18-19	0.49522157032210856
1102	20-21	0.4229355991911703
1102	22-23	0.35954679920610033
1102	24-25	0.28605312438732255
1102	26-27	0.34906036511566185
1102	28-29	0.23366432902638934
1102	30-31	0.2057199664586875
1102	32-33	0.17282928895441785
1102	34-35	0.21674120752135195
1102	36-37	0.17414675574151772
1102	38-39	0.1685675278395493
1102	40-41	0.13808237239203436
1102	42-43	0.1946670851010417
1102	44-45	0.18450447129314895
1102	46-47	0.18985848363447744
1102	48-49	0.17539508260725611
1102	50-51	0.0830770865987347
1102	52-53	0.08345204897373648
1102	54-55	0.10873575881214848
1102	56-57	0.06275685589985258
1102	58-59	0.041933548775741514
1102	60-61	0.0407092251379666
1102	62-63	0.06658306832225591
1102	64-65	0.17181230343829412
1102	66-67	0.20521026899348627
1102	68-69	0.16955043330619546
1102	70-71	0.25388763100505685
1102	72-73	0.2218069444956967
1102	74-75	0.17905231843115388
1102	76-77	0.15053357055161243
1102	78-79	0.14740008755620337
1102	80-81	0.13838831678006613
1102	82-83	0.1132909472200403
1102	84-85	0.06669394454383593
1102	86-87	0.11571789521955722
1102	88-89	0.11744489916372558
1102	90-91	0.12747225888661262
1102	92-93	0.09616213831570519
1102	94-95	0.10925753132217508
1102	96-97	0.09924712141683045
1102	98-99	0.09448761002641959
1102	100	0.0414683976021486
1103	1	0.24076755526558458
1103	2	0.2395529398480747
1103	3	0.2208629639837305
1103	4	0.1802888705582859
1103	5	0.1679597700275295
1103	6	0.1304496398285906
1103	7	0.20820265398506876
1103	8	0.2538903380161628
1103	9	0.24712819397672803
1103	10-11	0.16562758614289663
1103	12-13	0.23288829695169255
1103	14-15	0.33762818596862587
1103	16-17	0.26193016222937615
1103	18-19	0.4103617258513381
1103	20-21	0.2591109714249882
1103	22-23	0.19211409377080457
1103	24-25	0.199818985809884
1103	26-27	0.28584661687971646
1103	28-29	0.04160812365932287
1103	30-31	0.0019213455737059348
1103	32-33	-0.0033429334738883654
1103	34-35	0.019349672006988783
1103	36-37	-0.052306819286116024
1103	38-39	-0.07875911735510499
1103	40-41	-0.02865721375722785
1103	42-43	-0.024941546076330923
1103	44-45	0.00537388089284363
1103	46-47	0.009807180806024718
1103	48-49	-0.0478345651081753
1103	50-51	-0.045230645524831914
1103	52-53	-0.132729925712173
1103	54-55	-0.14401190053786905
1103	56-57	-0.18175497961702192
1103	58-59	-0.1331573921724747
1103	60-61	-0.15448841660175106
1103	62-63	0.009488729677936902
1103	64-65	-0.012120118317564987
1103	66-67	-0.024952191039478322
1103	68-69	0.008443436163073414
1103	70-71	0.01271882457891138
1103	72-73	-0.039084141809784256
1103	74-75	-0.006794648481456278
1103	76-77	-0.01655935946197573
1103	78-79	-0.017456764663904778
1103	80-81	-2.1393345829068267E-4
1103	82-83	-0.033099789898052734
1103	84-85	-0.09784864322543285
1103	86-87	-0.04886821126472185
1103	88-89	-0.002925516801408179
1103	90-91	-0.0027810444299376513
1103	92-93	-0.024407773080032058
1103	94-95	0.01454279389685098
1103	96-97	-0.07926827542072701
1103	98-99	-0.06634187233390776
1103	100	-0.09851537231147134
1104	1	0.2251442900264422
1104	2	0.19100606619758054
1104	3	0.21389771999059448
1104	4	0.20924877556596755
1104	5	0.17429886261507477
1104	6	0.06962661337298215
1104	7	0.1517848758388638
1104	8	0.22843667342358032
1104	9	0.04073374082391723
1104	10-11	0.11552964390720888
1104	12-13	0.21409556837667054
1104	14-15	0.3349663438051067
1104	16-17	0.37951774002890204
1104	18-19	0.3496678347198099
1104	20-21	0.24677686239441954
1104	22-23	0.19489911733990795
1104	24-25	0.22792394953992812
1104	26-27	0.2541956390350961
1104	28-29	0.1314939474863266
1104	30-31	0.10862486664146331
1104	32-33	0.05334296580177522
1104	34-35	0.06354104565845375
1104	36-37	0.02373184191733202
1104	38-39	-0.0035382408976225577
1104	40-41	0.015273099402968171
1104	42-43	0.0036619122291732253
1104	44-45	-0.010491318435146724
1104	46-47	-0.012474764610573175
1104	48-49	-0.07307994148858654
1104	50-51	-0.008134571101404475
1104	52-53	-0.0957774470948003
1104	54-55	-0.09179891985873212
1104	56-57	-0.06994627178540114
1104	58-59	-0.03958014072529892
1104	60-61	0.006617098060857529
1104	62-63	-0.038027000751075235
1104	64-65	0.03112487909891115
1104	66-67	0.09348644069241274
1104	68-69	0.09088952144630014
1104	70-71	0.05076015868557704
1104	72-73	0.04162579030516156
1104	74-75	0.07779923992475801
1104	76-77	0.09308231683676738
1104	78-79	0.05701513232156685
1104	80-81	0.02313709581714818
1104	82-83	0.038719770054584046
1104	84-85	0.011118107344433525
1104	86-87	-0.015815274575388116
1104	88-89	-0.002031546601788392
1104	90-91	6.29684415656584E-4
1104	92-93	-0.007453462015046597
1104	94-95	0.01574515114222308
1104	96-97	0.013778534674806053
1104	98-99	-0.0056624404553460295
1104	100	0.009743461098423722
1105	1	0.16828047061170537
1105	2	0.1382838671385862
1105	3	0.1087251428150573
1105	4	0.1816200090756368
1105	5	0.11826178448634295
1105	6	0.012597005073928358
1105	7	-0.0090838143214782
1105	8	0.17016246597851392
1105	9	0.106766929526799
1105	10-11	0.13440936136640147
1105	12-13	0.1584157178875003
1105	14-15	0.24449207824865482
1105	16-17	0.19428695155376374
1105	18-19	0.26723603578158617
1105	20-21	0.11086695016570758
1105	22-23	0.040276435000841104
1105	24-25	0.00994190025448205
1105	26-27	0.08210813149452889
1105	28-29	-0.05635705839222993
1105	30-31	-0.037994793769868807
1105	32-33	-0.08313386201074024
1105	34-35	-0.037320145957536965
1105	36-37	-0.08757976547966706
1105	38-39	-0.14698566353595766
1105	40-41	-0.1110027960287212
1105	42-43	-0.15864109250648184
1105	44-45	-0.1292486846784584
1105	46-47	-0.1323479738536193
1105	48-49	-0.1295975477684621
1105	50-51	-0.20223640753303584
1105	52-53	-0.13789941656759197
1105	54-55	-0.17526361691000147
1105	56-57	-0.1903021260902058
1105	58-59	-0.18917917187027555
1105	60-61	-0.15561061351959893
1105	62-63	-0.11703858246699994
1105	64-65	-0.06092704620677125
1105	66-67	-0.021730801678156553
1105	68-69	-0.0591356387020916
1105	70-71	-0.07421641936139167
1105	72-73	-0.08823415504114607
1105	74-75	-0.056085945551505034
1105	76-77	-0.046563403572445594
1105	78-79	0.0016663346259662148
1105	80-81	-0.010330860947988185
1105	82-83	-0.006832933738671443
1105	84-85	-0.06383974774556123
1105	86-87	0.007755600095816106
1105	88-89	-0.02224930172928552
1105	90-91	-0.04224569113962673
1105	92-93	-0.03858971007397827
1105	94-95	-0.019743490947227826
1105	96-97	7.327923470867859E-4
1105	98-99	0.0462140701171343
1105	100	-0.03341325123219008
1106	1	0.03694294810869536
1106	2	0.11681747493073757
1106	3	0.1557891788390826
1106	4	0.07308913857512067
1106	5	0.14850017040461694
1106	6	0.028573383425651855
1106	7	0.14064560094838185
1106	8	0.19778856455361904
1106	9	0.20294022958569258
1106	10-11	0.19053029565152713
1106	12-13	0.21952120694724186
1106	14-15	0.2860009332643614
1106	16-17	0.22769625513920033
1106	18-19	0.2742932230159454
1106	20-21	0.1731550229927663
1106	22-23	0.21596265087735134
1106	24-25	0.15129560218136362
1106	26-27	0.255659943435667
1106	28-29	0.08064966390127637
1106	30-31	0.009902056081187993
1106	32-33	0.016727753066462014
1106	34-35	0.04069665139470402
1106	36-37	0.021324093725866078
1106	38-39	0.009596199847489828
1106	40-41	0.020717773366456527
1106	42-43	0.05811226579895745
1106	44-45	0.0194705447959862
1106	46-47	0.042329717616897966
1106	48-49	0.038419131726840305
1106	50-51	0.03305465588991652
1106	52-53	0.05267309768258244
1106	54-55	0.027699875271419927
1106	56-57	0.005042376635326917
1106	58-59	-0.0010999793093269261
1106	60-61	9.692426341842975E-4
1106	62-63	-0.06934130447656983
1106	64-65	0.05516019632160507
1106	66-67	0.055493796037080045
1106	68-69	0.05567856588175246
1106	70-71	0.04383407347722823
1106	72-73	0.036988466054683755
1106	74-75	-0.014734361387539252
1106	76-77	0.07025340929784463
1106	78-79	0.08783036358942553
1106	80-81	0.08121535430791837
1106	82-83	0.07063595942308609
1106	84-85	0.09785371753925176
1106	86-87	0.12077071490135083
1106	88-89	0.13477978734134055
1106	90-91	0.11884737513505428
1106	92-93	0.14506338681150055
1106	94-95	0.12349571843701668
1106	96-97	0.13806309396345284
1106	98-99	0.1397919137248138
1106	100	0.08926267858236514
1107	1	0.054381164330830956
1107	2	0.035471969154276195
1107	3	0.03871545780289054
1107	4	0.08903325028917664
1107	5	0.08066451310867251
1107	6	-0.015375744715605322
1107	7	0.02185330825212617
1107	8	0.15258414119892194
1107	9	0.17614004202602018
1107	10-11	0.10167971012295851
1107	12-13	0.12038797669825385
1107	14-15	0.24050221287318863
1107	16-17	0.18746190654719896
1107	18-19	0.19470906568427182
1107	20-21	0.11020195997329552
1107	22-23	0.04007510191841135
1107	24-25	0.024817587318793244
1107	26-27	0.11960291902816067
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2216	18-19	0.626249135151987
2216	20-21	0.5656498587856547
2216	22-23	0.7854597936511709
2216	24-25	0.863835666909246
2216	26-27	0.9670905431157379
2216	28-29	1.0081377499477
2216	30-31	1.0983446976104005
2216	32-33	1.155026439247223
2216	34-35	1.136061122021367
2216	36-37	1.1397569745223564
2216	38-39	1.1563978266600614
2216	40-41	1.2286548537871695
2216	42-43	1.1914174079261066
2216	44-45	1.2226532052907224
2216	46-47	1.1581249511822342
2216	48-49	1.1393251378060754
2216	50-51	1.0950315200666374
2216	52-53	1.1124060349737128
2216	54-55	1.0782134062922921
2216	56-57	1.0964111160515024
2216	58-59	1.069622961227946
2216	60-61	1.0531700913529676
2216	62-63	0.907513122884712
2216	64-65	0.8586175624893144
2216	66-67	0.9072820254769667
2216	68-69	0.8480392562609502
2216	70-71	0.7880453832624994
2216	72-73	0.7722316065401138
2216	74-75	0.8016567207329182
2216	76-77	0.7635302988277353
2216	78-79	0.7346137440273885
2216	80-81	0.7270407108911314
2216	82-83	0.6903474056808321
2216	84-85	0.6444803229712335
2216	86-87	0.5639416249538165
2216	88-89	0.4431120682564931
2216	90-91	0.42981532256492017
2216	92-93	0.38806901443937036
2216	94-95	0.3323676784312113
2216	96-97	0.3075522871151577
2216	98-99	0.30460349638368456
2216	100	0.5719093135094084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	303.0
6	3482.0
7	10805.0
8	18177.0
9	28447.0
10	41673.0
11	52559.0
12	59159.0
13	64063.0
14	71393.0
15	82244.0
16	96418.0
17	113933.0
18	137766.0
19	162153.0
20	188989.0
21	218313.0
22	248461.0
23	291519.0
24	386321.0
25	444470.0
26	497457.0
27	806970.0
28	695201.0
29	1140532.0
30	1109154.0
31	1361660.0
32	1781065.0
33	2763232.0
34	2367279.0
35	3757324.0
36	776194.0
37	249666.0
38	72813.0
39	21834.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.155066998141145	31.28426580659039	26.04631199851538	26.514355196753087
2	12.983362795934136	34.72992306690293	28.03263795830314	24.254076178859798
3	11.455278751784261	37.23870491399738	25.35384171609536	25.952174618122996
4	10.09109238097775	37.61438731616821	25.20737422464524	27.0871460782088
5	9.716888391454914	36.68351635899536	26.499748521945186	27.099846727604536
6	9.905306435816625	33.748812317250426	28.0346455966603	28.311235650272643
7	13.600176411703647	38.9165679395286	23.098999389397626	24.384256259370133
8	11.16885476619237	35.460144005943214	20.90756008837284	32.463441139491586
9	9.349556888351211	34.76730463333193	21.799836728721882	34.08330174959498
10-11	13.155417239820816	49.36430209579847	21.53743326537487	15.94284739900584
12-13	12.912181254825	49.653598856146516	21.624342913314145	15.809876975714335
14-15	13.54107183131113	48.80962107764014	21.356807083345387	16.29250000770335
16-17	13.465805123911132	48.709251386379506	21.6805018847366	16.144441604972766
18-19	13.810423220106005	47.811028256189	22.347179683987676	16.031368839717324
20-21	13.349077826674463	47.72033937095615	22.632102435308113	16.29848036706128
22-23	13.615138528639822	48.13290618658712	22.942958912306526	15.30899637246653
24-25	13.649805885286941	48.08417455672883	22.62852085761548	15.637498700368745
26-27	14.633016716796515	48.06154788907951	22.090059281112147	15.215376113011828
28-29	14.369231743331905	48.62847057076462	22.343355343049097	14.658942342854377
30-31	14.53579309773803	48.59275187248579	22.217052694000937	14.654402335775247
32-33	14.795197224169595	48.61954710857461	21.871028115043732	14.71422755221207
34-35	14.890651680617683	48.697075122182596	21.74601515601969	14.666258041180026
36-37	15.256933587275537	48.56951011758097	21.48907361689815	14.68448267824535
38-39	15.52785049575287	48.60893636406016	21.475510749553926	14.387702390633045
40-41	15.712727080617798	48.8272089258725	21.417541076152734	14.042522917356967
42-43	15.849576999983375	48.87526642742691	21.539355815985893	13.735800756603819
44-45	15.96652500199231	48.70010255151723	21.506389323072266	13.826983123418188
46-47	15.971176365138273	48.533339973143555	21.694297670346046	13.801185991372122
48-49	15.903067271292054	48.57904203987961	21.92026542870972	13.597625260118615
50-51	16.132719125968652	48.51137181355163	22.051011204203505	13.304897856276213
52-53	16.218186245914584	48.501023489630605	22.17894512542485	13.10184513902996
54-55	16.260123448470136	48.61037473681867	22.113583407037027	13.015918407674171
56-57	16.195935512504033	48.61666677434794	21.918338501205948	13.269059211942086
58-59	16.060688606213933	48.520161016977795	21.992017040015888	13.427133336792387
60-61	16.131755925641095	48.285122284978854	22.2085193692556	13.374602420124452
62-63	16.183679727410667	47.84677137593304	22.413398167397325	13.556150729258972
64-65	16.048475186382095	47.843936055479276	22.407076830323156	13.700511927815473
66-67	15.69572806231077	47.811357541444096	22.638223196766774	13.854691199478358
68-69	15.588732154689994	47.79035808431998	22.4389784366546	14.181931324335423
70-71	15.343229109991155	47.93265752165014	22.526051901003335	14.198061467355366
72-73	15.33509202307634	48.224053031056044	22.487791386528727	13.953063559338883
74-75	15.224577206211245	48.5264590287124	22.354226347502998	13.894737417573353
76-77	14.812331022301855	49.027466011749475	22.378953514975922	13.781249450972746
78-79	14.49418532717004	49.545196707024516	22.195734630144106	13.764883335661338
80-81	14.154313078050187	50.29415720211756	21.888957209314068	13.662572510518181
82-83	13.837577443870078	50.97835515305681	21.802583716215356	13.381483686857765
84-85	13.501618639921013	51.73408914344423	21.679956719308258	13.084335497326505
86-87	13.277389149952908	52.340724578977316	21.484512180272507	12.897374090797264
88-89	13.149209556370037	52.829855265725634	21.31177642212671	12.709158755777619
90-91	13.162618032981083	53.24584718408792	21.056574645445988	12.534960137485005
92-93	13.267254140900132	53.54959732797969	20.71837818603872	12.464770345081451
94-95	13.466260210099087	53.5903412210406	20.485938318723225	12.457460250137084
96-97	13.674692206929267	53.44110392970146	20.37485869963331	12.509345163735967
98-99	13.940997307798646	53.13275491435218	20.262953954554984	12.6632938232942
100	14.48445979573951	52.41624354684495	20.264211453453736	12.835085203961796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1921071.0
1	1931476.0
2	1234669.0
3	325266.0
4	118894.5
5	111158.0
6	95128.0
7	68744.5
8	45456.5
9	33022.0
10	30738.5
11	34370.0
12	41776.0
13	53166.0
14	69056.5
15	89531.0
16	113068.0
17	138763.0
18	166231.0
19	194404.0
20	221929.0
21	246708.5
22	268664.0
23	288724.0
24	306189.0
25	320662.5
26	331178.0
27	336980.0
28	339686.0
29	339661.0
30	338183.5
31	336768.5
32	334764.0
33	333736.5
34	334260.5
35	334666.0
36	335874.0
37	338431.5
38	338276.0
39	334637.0
40	331893.0
41	331323.0
42	334059.0
43	343982.5
44	364721.5
45	396335.5
46	433594.5
47	469888.5
48	497812.5
49	510715.0
50	508343.0
51	493926.0
52	471448.5
53	440458.0
54	399115.0
55	347959.0
56	288409.5
57	225384.0
58	167015.5
59	119411.0
60	84152.5
61	60217.5
62	44390.5
63	34007.0
64	27091.5
65	22181.5
66	18441.0
67	15460.0
68	13148.5
69	11236.0
70	9635.0
71	8252.0
72	7108.0
73	6258.0
74	5521.5
75	5003.0
76	4601.0
77	4155.5
78	3776.0
79	3460.5
80	3134.5
81	2917.5
82	2729.0
83	2514.5
84	2377.0
85	2294.5
86	2231.0
87	2130.5
88	2086.5
89	1993.5
90	1903.5
91	1897.0
92	1846.0
93	1760.5
94	1695.5
95	1610.0
96	1516.5
97	1394.0
98	1246.5
99	1091.5
100	956.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.367496054144914E-4
2	6.212379692297355E-4
3	2.0376605390735322E-4
4	9.939807507675768E-6
5	3.677728777840034E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.7334470646108362E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.484951876918942E-5
22-23	1.9879615015351537E-5
24-25	1.0933788258443345E-4
26-27	1.6897672763048805E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	5.4668941292216724E-5
42-43	7.70335081844872E-5
44-45	3.727427815378413E-5
46-47	2.832845139687594E-4
48-49	2.484951876918942E-5
50-51	2.484951876918942E-5
52-53	7.454855630756825E-6
54-55	2.98194225230273E-5
56-57	1.9879615015351537E-5
58-59	2.98194225230273E-5
60-61	0.0
62-63	1.9879615015351537E-5
64-65	7.70335081844872E-5
66-67	1.6897672763048805E-4
68-69	3.528631665224897E-4
70-71	8.622783012908727E-4
72-73	3.677728777840034E-4
74-75	9.641613282445494E-4
76-77	0.0014934560780282841
78-79	8.076093599986563E-4
80-81	0.001192776900921092
82-83	0.0012474458422133089
84-85	0.0018065600145200707
86-87	0.0023060353417807783
88-89	0.002397978561226779
90-91	0.002581865000118781
92-93	0.00227870087113467
94-95	0.0011281681521211997
96-97	0.002050085298458127
98-99	0.002012811020304343
100	0.001669887661289529
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.0121114E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.68487460969442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.84269651360168	67.27101932613144
2	5.427445354358529	7.781314793563192
3	0.4591452265717549	0.9874130398330794
4	0.0906369834844953	0.25989203184347953
5	0.036924127482616455	0.1323450724331866
6	0.027356905600276267	0.11766458085990307
7	0.017007225496364006	0.08534125790159564
8	0.013533782689122543	0.0776134012051682
9	0.010451110534111954	0.06742678933528846
>10	0.06382273053981913	0.845430299187688
>50	0.0054053708295566305	0.2676250665869215
>100	0.0035935435682238333	0.5239180198797098
>500	5.280332039551917E-4	0.2650961099969606
>1k	8.494651785644219E-4	1.3468580685479636
>5k	1.3335942277664013E-4	0.7569492001113668
>10k+	4.7026743821236257E-4	19.214092942583076
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	812536	4.0382257165284186	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	241892	1.2021799588233535	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	197621	0.9821573497371965	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186945	0.9290986572612231	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	136743	0.6795995490110537	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114885	0.5709673927596653	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106922	0.5313920491678542	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99649	0.4952459391661913	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93800	0.46617697210999354	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92941	0.46190782478544673	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89152	0.4430768594621551	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79238	0.39380523364660625	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78678	0.391022087544457	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74286	0.36919427025760104	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72542	0.3605267581109078	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65050	0.32329223918715433	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65043	0.32325744986087745	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61626	0.30627528873401344	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56684	0.2817140243825466	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53490	0.2658401517927884	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49525	0.2461344834088212	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49073	0.24388808691208647	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48158	0.23934062497732483	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41337	0.2054409114723966	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36338	0.18059636260696102	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35122	0.17455295964229417	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34201	0.16997567828500945	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33073	0.16436962685068035	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32067	0.15936990367431944	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31650	0.157297453808969	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30825	0.15319728321205278	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26747	0.13293001570390187	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26658	0.13248769426981033	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25981	0.12912306942846208	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24143	0.11998838632890803	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23346	0.11602737303709923	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23318	0.11588821573199178	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22414	0.11139542273852233	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21846	0.10857251740634241	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21370	0.10620684321951557	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21248	0.10560051496154735	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20994	0.10433815940807253	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20640	0.10257881347921392	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20441	0.10158980263220017	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014710915111360136	0.0	0.0	4.969903753837884E-6	0.0
2	0.003459053012671167	0.0	0.0	4.969903753837884E-6	0.0
3	0.005864486429528703	0.0	0.0	2.9819422523027305E-5	0.0
4	0.009318569538446033	0.0	0.0	5.4668941292216724E-5	0.0
5	0.015113477315421005	0.0	0.0	7.454855630756825E-5	0.0
6	0.025281900395773316	0.0	0.0	7.951846006140615E-5	0.0
7	0.03714009075243051	0.0	0.0	8.448836381524403E-5	0.0
8	0.08006017957057447	0.0	0.0	9.939807507675768E-5	0.0
9	0.2367016060840369	0.0	0.0	1.0436797883059557E-4	0.0
10-11	0.5803704506619266	0.0	0.0	1.118228344613524E-4	0.0
12-13	1.0231192964763283	0.0	0.0	1.242475938459471E-4	4.969903753837884E-6
14-15	1.5392785906386695	0.0	0.0	1.29217497599785E-4	4.969903753837884E-6
16-17	2.1096172905734742	0.0	0.0	1.540670163689744E-4	4.969903753837884E-6
18-19	2.749698649885886	0.0	0.0	1.6649177575356913E-4	4.969903753837884E-6
20-21	3.592999373692729	0.0	0.0	1.7643158326124486E-4	9.939807507675768E-6
22-23	4.5393585066910305	0.0	0.0	1.838864388920017E-4	9.939807507675768E-6
24-25	5.495563019025687	0.0	0.0	1.9134129452275854E-4	9.939807507675768E-6
26-27	6.450838159358374	0.0	0.0	2.062510057842722E-4	9.939807507675768E-6
28-29	7.475632313399745	0.0	0.0	2.2116071704578584E-4	9.939807507675768E-6
30-31	8.584986894860792	0.0	0.0	2.4601023581497525E-4	9.939807507675768E-6
32-33	9.821113781274734	0.0	0.0	2.658898508303268E-4	9.939807507675768E-6
34-35	10.952427882472115	0.0	0.0	2.8079956209184045E-4	9.939807507675768E-6
36-37	11.844702037869276	0.0	0.0	2.9073936959951623E-4	9.939807507675768E-6
38-39	12.522415508405746	0.0	0.0	3.3546850338405716E-4	9.939807507675768E-6
40-41	13.109900376291293	0.0	0.0	3.528631665224897E-4	9.939807507675768E-6
42-43	13.587756622222805	0.0	0.0	3.777126852916792E-4	9.939807507675768E-6
44-45	13.978843318516063	2.484951876918942E-6	0.0	4.174719153223823E-4	9.939807507675768E-6
46-47	14.319314030028357	4.969903753837884E-6	0.0	4.3486657846081485E-4	9.939807507675768E-6
48-49	14.639199400192256	4.969903753837884E-6	0.0	4.771107603684368E-4	1.4909711261513652E-5
50-51	14.962476729668149	4.969903753837884E-6	0.0	5.019602791376263E-4	1.4909711261513652E-5
52-53	15.306592865583884	9.939807507675768E-6	0.0	5.56629220429843E-4	1.4909711261513652E-5
54-55	15.660002721519295	9.939807507675768E-6	0.0	6.485724398758439E-4	1.4909711261513652E-5
56-57	16.033664935251597	1.2424759384594711E-5	0.0	6.83361766152709E-4	1.4909711261513652E-5
58-59	16.427422954812542	1.4909711261513652E-5	0.0	7.032413811680606E-4	1.4909711261513652E-5
60-61	16.83713933532706	1.7394663138432594E-5	0.0	7.206360443064931E-4	1.4909711261513652E-5
62-63	17.233307758208618	1.9879615015351537E-5	0.0	7.554253705833584E-4	1.4909711261513652E-5
64-65	17.612262919438756	1.9879615015351537E-5	0.0	7.852447931063856E-4	1.7394663138432594E-5
66-67	17.966448080359765	1.9879615015351537E-5	0.0	8.12579263752494E-4	1.9879615015351537E-5
68-69	18.28573209216945	2.2364566892270476E-5	0.0	8.349438306447646E-4	1.9879615015351537E-5
70-71	18.583715096490184	2.7334470646108362E-5	0.0	8.57308397537035E-4	1.9879615015351537E-5
72-73	18.872916280877888	3.478932627686519E-5	0.0	8.821579163062244E-4	1.9879615015351537E-5
74-75	19.154759522758035	3.478932627686519E-5	0.0	9.194321944600085E-4	1.9879615015351537E-5
76-77	19.43414266228003	3.478932627686519E-5	0.0	9.716161838753063E-4	1.9879615015351537E-5
78-79	19.712201322451627	3.478932627686519E-5	0.0	0.001028770077044442	1.9879615015351537E-5
80-81	19.987618478778064	3.727427815378413E-5	0.0	0.0010834390183366586	1.9879615015351537E-5
82-83	20.265319305879387	3.975923003070307E-5	0.0	0.0011256832002442807	1.9879615015351537E-5
84-85	20.54611638301935	3.975923003070307E-5	0.0	0.001160472526521146	1.9879615015351537E-5
86-87	20.831065814745642	3.975923003070307E-5	0.0	0.0011728972859057405	1.9879615015351537E-5
88	21.05034045331685	3.975923003070307E-5	0.0	0.0011828370934134163	1.9879615015351537E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGACG	10240	0.0	30.109035	8
TACCCGA	8890	0.0	29.817434	6
ACCCGAC	9485	0.0	29.037098	7
TGACGCC	910	0.0	26.85704	3
CCGCCAA	3070	0.0	26.791428	5
GTACGCC	635	0.0	26.645565	3
GCCGCCA	1500	0.0	26.319897	4
GCCGACG	1025	0.0	24.760695	7
CCGACGC	12210	0.0	24.67376	9
CTACCCG	9690	0.0	24.639742	5
TGAAAAA	24180	0.0	24.588713	1
TGAACCG	8250	0.0	23.756098	12-13
TACGCCG	905	0.0	23.370075	4
GTCCGCC	1190	0.0	23.302433	3
TTGCGCC	1075	0.0	23.172003	3
AGACGAC	1990	0.0	22.909288	6
TACGACG	4595	0.0	22.809317	7
TCGCGAC	645	0.0	22.588892	6
TTGCCGC	1115	0.0	22.340832	2
GACACGC	1200	0.0	22.325026	2
>>END_MODULE