FastQCFastQC Report
Thu 26 May 2016
SRR1248456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272716550
Sequences flagged as poor quality0
Sequence length20-99
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGAAGGTGTTAGTGGGAATGTTTGTGATGAGATTTTTAGTTGAAATA13258850.48617694819034635No Hit
TTGTGATGTTTTTTATTTTTTGGATGTTTTGTTTGTTTGTTAGGTTATGA9902090.36309090885756656No Hit
TGGTGAGTAGGTTAAGGGTTAATAGTGTAATTGAATTATAGTGAAATTAT8524090.3125622555726816No Hit
TTGTTTGTGGGTATTTTTATTATTATTGAGATTATTTGAGATTAATTGAG5195090.19049412292726642No Hit
TGGTAGGAATTGTGATAATTATAGTGGTTGATGTAAAGTAAGTTTGTGTG3410460.12505511675034023No Hit
TGGTTGTGTTTGGATATATGATTGTTATAGTTATTGAGGAATATTTAGAG2804600.10283937663482469No Hit
TTGTTTAGTTGTTTATGTTAGTTTTTAATTAAGGAATAAGTGATTATGTT2736980.10035987914924856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAC149950.084.9231641
CCCCCGG104050.082.2129441
CCGCCGG53950.078.4837651
CACGACG1714000.075.198092
ACGCGAC48300.074.004991
GCGACGC43450.073.700523
ACGACGC1816700.073.289813
CGCGACG44650.071.415512
ACACGAC1886150.071.170431
CGTGTCC74950.070.5017559
GGGTACC81300.070.423521
CCTCGTG76650.070.2980046
CCCTCGG34900.069.401851
GCCGGTC196800.066.601873
CGCCGGT98850.066.572092
CGCCCGG29300.066.0097051
CTATGGC39350.065.195614
GGTACCT87250.064.241492
GGGCCGG50650.062.7202151
GGACTAT135450.060.018321
Produced by FastQC (version 0.11.5)