##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248456_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272716550
Sequences flagged as poor quality	0
Sequence length	20-99
%GC	26
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.886447654166936	33.0	31.0	34.0	30.0	34.0
2	32.10360751483546	34.0	31.0	34.0	30.0	34.0
3	32.29153509018796	34.0	31.0	34.0	30.0	34.0
4	35.24858907902729	37.0	35.0	37.0	33.0	37.0
5	35.61314745291402	37.0	35.0	37.0	33.0	37.0
6	35.73190092790482	37.0	35.0	37.0	35.0	37.0
7	35.766889937556044	37.0	35.0	37.0	35.0	37.0
8	35.776660730711065	37.0	35.0	37.0	35.0	37.0
9	37.58710148687346	39.0	38.0	39.0	35.0	39.0
10-11	37.555867991876546	39.0	38.0	39.0	35.0	39.0
12-13	37.56017383066778	39.0	38.0	39.0	35.0	39.0
14-15	38.96957574815316	41.0	39.0	41.0	36.0	41.0
16-17	38.92398555569876	40.0	39.0	41.0	36.0	41.0
18-19	38.89289018029892	40.0	39.0	41.0	35.5	41.0
20-21	38.86438897920879	40.0	39.0	41.0	35.0	41.0
22-23	38.77708890061905	40.0	39.0	41.0	35.0	41.0
24-25	38.69832034480494	40.0	39.0	41.0	35.0	41.0
26-27	38.56358144358631	40.0	38.5	41.0	34.0	41.0
28-29	38.40545275045529	40.0	38.0	41.0	34.0	41.0
30-31	38.32820460663406	40.0	38.0	41.0	34.0	41.0
32-33	38.221668374115566	40.0	38.0	41.0	33.5	41.0
34-35	38.089051727689174	40.0	38.0	41.0	33.0	41.0
36-37	37.936188003774554	40.0	38.0	41.0	33.0	41.0
38-39	38.08351631047909	40.0	38.0	41.0	33.0	41.0
40-41	37.995040654349864	40.0	38.0	41.0	33.0	41.0
42-43	37.87319681378443	40.0	38.0	41.0	33.0	41.0
44-45	37.72155684733278	40.0	37.5	41.0	32.0	41.0
46-47	37.593247170648254	40.0	37.0	41.0	32.0	41.0
48-49	37.45207322212403	40.0	37.0	41.0	31.0	41.0
50-51	36.62662854755405	39.0	36.0	40.0	30.5	40.5
52-53	36.66774080385264	39.0	36.0	40.0	30.5	40.5
54-55	37.173375972486795	39.5	36.5	40.5	31.0	41.0
56-57	37.42877695310379	40.0	36.5	41.0	32.0	41.0
58-59	37.28286449810584	39.5	36.0	41.0	31.0	41.0
60-61	37.08896745934403	39.0	36.0	41.0	31.0	41.0
62-63	36.83389578755265	39.0	35.0	41.0	31.0	41.0
64-65	36.57186909722931	39.0	35.0	41.0	30.5	41.0
66-67	36.2995813324876	38.0	35.0	40.0	30.0	41.0
68-69	36.02001236740966	37.5	35.0	40.0	30.0	41.0
70-71	35.673544086215685	37.0	35.0	40.0	29.0	41.0
72-73	35.36668819270005	36.5	34.0	39.0	29.0	41.0
74-75	35.05632756606643	36.0	34.0	39.0	29.0	41.0
76-77	34.70607341787088	36.0	34.0	39.0	29.0	40.5
78-79	34.36169810255066	35.0	34.0	37.5	28.5	40.0
80-81	34.03723385563624	35.0	34.0	37.0	28.0	39.0
82-83	33.74523982014297	35.0	33.5	37.0	27.5	39.0
84-85	33.43406103699975	35.0	33.0	36.0	27.0	38.5
86-87	33.140164234764214	35.0	33.0	36.0	26.5	37.0
88-89	32.89370876574145	35.0	33.0	36.0	26.0	37.0
90-91	32.684351561114404	35.0	32.5	35.5	26.0	37.0
92-93	32.51568048678175	35.0	32.0	35.0	26.0	36.0
94-95	32.40402784551926	35.0	32.0	35.0	26.0	36.0
96-97	32.42931209815359	35.0	32.0	35.0	26.0	36.0
98-99	32.64987542147669	35.0	32.0	35.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	6.0
6	288.0
7	3840.0
8	14212.0
9	30247.0
10	52211.0
11	82462.0
12	123255.0
13	178053.0
14	239737.0
15	303577.0
16	362615.0
17	413086.0
18	465905.0
19	558568.0
20	803949.0
21	963079.0
22	1104672.0
23	1261040.0
24	1451202.0
25	1676022.0
26	1951784.0
27	2268084.0
28	2669532.0
29	3203400.0
30	3909206.0
31	4887032.0
32	6290396.0
33	8444881.0
34	1.2091893E7
35	1.8583469E7
36	3.2474075E7
37	6.0425124E7
38	7.3503615E7
39	3.134539E7
40	580641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.91839604494785	12.741173075543616	43.69309379397823	10.647337085530314
2	34.23215295895572	12.436597294794444	41.93420636688202	11.397043379367817
3	44.10677936772743	14.669614312077783	30.79960783770582	10.42399848248897
4	8.584944449888734	16.009115729885607	60.34766124785244	15.058278572373213
5	22.354475049827705	30.956235677773225	39.45472030868443	7.2345689637146435
6	20.967216818032135	29.956906161036162	42.62504051410859	6.45083650682311
7	20.745877246258985	28.817598476430522	43.1829210716542	7.253603205656289
8	21.644345861481348	27.065027978548766	44.27468107354305	7.015945086426832
9	20.552342612764225	28.562377860381368	43.25460372274657	7.630675804107842
10-11	20.231966601587022	27.324769336930434	45.102919536125555	7.3403445253569926
12-13	19.37502190923588	28.793762945747815	45.3659278513859	6.465287293630401
14-15	18.799626713753064	29.89194184046075	44.224155317375605	7.084276128410585
16-17	19.459489181702118	28.384162343946777	45.58183836344865	6.574510110902462
18-19	19.15355459676229	28.080275479765877	45.80601807900646	6.960151844465376
20-21	18.765958387288002	29.425453330621664	45.37824689991113	6.430341382179194
22-23	19.81447659189488	28.55438525494981	45.16464425338912	6.466493899766193
24-25	18.766519940151618	29.292369963073746	45.602165608946216	6.33894448782842
26-27	18.916966285109314	29.120633464508742	45.34511660361102	6.617283646770927
28-29	18.749810975691624	29.01919747665757	45.825282425753564	6.405709121897239
30-31	19.131766632646645	28.709906379663224	45.67714838704063	6.481178600649505
32-33	18.891241665189806	29.164179506128946	45.48393577683781	6.460643051843438
34-35	19.562467374260077	29.555445474146065	44.6473793182214	6.234707833372457
36-37	19.148252226417146	28.351005922095183	46.38783700948495	6.11290484200272
38-39	19.44514749311386	28.948837356725132	45.644893744661836	5.961121405499173
40-41	18.72777324078712	29.53911017199889	45.77226923255521	5.960847354658778
42-43	19.771026335992836	28.665834677270652	45.6376252561007	5.925513730635822
44-45	19.615755042382172	29.47463801010667	45.14541198414197	5.764194963369183
46-47	19.1664611727036	29.10958840945585	45.91894060024005	5.805009817600505
48-49	19.149241156652526	29.091128391639753	46.08940188510284	5.67022856660488
50-51	19.388458654099676	29.94791388869224	44.955246405651685	5.708381051556394
52-53	19.95385364447562	28.625265100578257	45.73412782549649	5.6867534294496345
54-55	19.136422733716735	29.735434638213565	45.42795140392503	5.700191224144666
56-57	19.290982586064796	29.417708856511588	45.60792317800886	5.6833853794147515
58-59	19.744095458228102	29.721875119939213	44.96296131276799	5.571068109064701
60-61	19.845893888017304	29.05744589583345	45.48055765273113	5.616102563418115
62-63	19.48239672245877	29.470541953282574	45.468040586966794	5.5790207372918665
64-65	19.083010445816086	29.756826059520197	45.61491250497479	5.545250989688922
66-67	19.206299667651095	28.945392573627878	46.38142883071688	5.4668789280041565
68-69	19.27685872425578	29.132995345208933	46.06341651672019	5.526729413815096
70-71	19.767389684513205	29.510987894389483	45.351408114288574	5.37021430680873
72-73	19.36436711404401	29.53209725830393	45.73200491137699	5.371530716275071
74-75	19.683544641402378	29.658587975929514	45.32053665329174	5.337330729376366
76-77	19.963415165470092	29.287733420662438	45.330765525176744	5.418085888690729
78-79	19.579259062005033	29.48149100765575	45.72570384963377	5.213546080705445
80-81	19.503967176081087	29.251728250100093	46.0251082585288	5.219196315290016
82-83	20.002825978550327	28.946108819454157	45.790858050441464	5.260207151554052
84-85	19.146702319458434	29.41947603097924	46.130029056876054	5.3037925926862695
86-87	19.95162842720988	29.332170590642704	45.66883788158378	5.047363100563646
88-89	19.813088554391044	29.29076094847768	45.80782038479537	5.088330112335906
90-91	19.53757351150475	29.303488374552234	46.12812095446921	5.030817159473808
92-93	19.58730462326984	29.750349093750977	45.540616169569645	5.121730113409533
94-95	20.02590729295212	28.653934450483913	46.26307398541206	5.057084271151908
96-97	19.521579428954784	28.318512851712907	47.09867944336111	5.061228275971202
98-99	19.94430927233692	24.150802072290485	51.04953324315912	4.855355412213474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334691.0
1	335854.5
2	350649.5
3	368928.5
4	377985.0
5	395394.5
6	432736.5
7	485770.0
8	561412.0
9	679635.0
10	854556.5
11	1118190.5
12	1540708.5
13	2152809.5
14	2999452.5
15	4015749.0
16	5155558.0
17	6516638.0
18	8065603.5
19	9926741.5
20	1.1676978E7
21	1.3237088E7
22	1.48477125E7
23	1.6026754E7
24	1.66556175E7
25	1.69520445E7
26	1.70631805E7
27	1.71446475E7
28	1.7101311E7
29	1.5721357E7
30	1.34342495E7
31	1.1618269E7
32	9964112.0
33	8472896.0
34	7116753.5
35	5798693.0
36	4766787.5
37	4052866.5
38	3368468.0
39	2673591.5
40	2165595.0
41	1767706.0
42	1437763.0
43	1159068.5
44	926250.0
45	738428.0
46	592151.5
47	477096.0
48	385464.0
49	318157.5
50	276154.5
51	232129.0
52	182392.0
53	145498.5
54	121632.0
55	107886.0
56	100936.5
57	100400.5
58	102724.0
59	109572.5
60	120954.5
61	129066.5
62	129287.5
63	122917.5
64	111903.0
65	97014.5
66	81661.5
67	66906.5
68	54757.0
69	44667.0
70	35110.5
71	29377.0
72	25277.5
73	19500.0
74	13918.5
75	10181.5
76	7766.5
77	6233.5
78	5049.0
79	3841.0
80	2884.0
81	2290.0
82	1994.5
83	1635.0
84	1254.0
85	1026.0
86	855.0
87	753.0
88	660.5
89	563.5
90	478.5
91	436.0
92	427.5
93	390.5
94	372.0
95	377.5
96	389.5
97	437.0
98	567.0
99	1328.0
100	1988.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06380250850195927
2	0.0027009728599162756
3	0.004439407876052993
4	0.012968776555731584
5	0.009492639885624836
6	0.03840140981542924
7	0.04048379168774319
8	0.05705337648191868
9	0.05064745795588864
10-11	0.029467225219738224
12-13	0.01064805197924365
14-15	7.243784801472445E-4
16-17	0.014223009201311764
18-19	0.013858161523383896
20-21	0.0024906401113794015
22-23	0.0014627475537579778
24-25	0.005364153388513507
26-27	0.001334057895097827
28-29	0.005168076314417572
30-31	0.0013606204146249582
32-33	0.0025522062431395636
34-35	0.0134026962856679
36-37	0.016054214747604725
38-39	0.021192004719181735
40-41	0.034553099686524556
42-43	0.03440098621978391
44-45	0.027890282707890436
46-47	0.027876489810659818
48-49	0.012083507224577213
50-51	0.004958216006817996
52-53	0.006445967932550579
54-55	0.017443988006919636
56-57	0.021361077997881558
58-59	0.02990961585545145
60-61	0.01213369171392791
62-63	0.0026397398071008587
64-65	7.423830186669297E-5
66-67	2.5040699279262968E-5
68-69	0.0033602656186324828
70-71	0.0033144035267490967
72-73	8.776420034986615E-4
74-75	0.0016326695956463192
76-77	0.020746254969189373
78-79	0.044141394334779295
80-81	0.013097272610717421
82-83	5.771690409831097E-4
84-85	3.1231365269363334E-4
86-87	0.009915893466125123
88-89	0.025265768403244786
90-91	0.04227705360794714
92-93	0.04959641900822856
94-95	0.013103573412044609
96-97	1.6861196893985006E-4
98-99	7.465113409919978E-5
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	228109.0
22-23	220150.0
24-25	221461.0
26-27	237049.0
28-29	221049.0
30-31	248799.0
32-33	267153.0
34-35	292101.0
36-37	275275.0
38-39	283360.0
40-41	294778.0
42-43	281563.0
44-45	297574.0
46-47	315684.0
48-49	319769.0
50-51	312431.0
52-53	311419.0
54-55	345650.0
56-57	352472.0
58-59	374995.0
60-61	415705.0
62-63	453632.0
64-65	460075.0
66-67	481739.0
68-69	488077.0
70-71	522303.0
72-73	556123.0
74-75	589798.0
76-77	621942.0
78-79	656124.0
80-81	723263.0
82-83	825554.0
84-85	939725.0
86-87	978131.0
88-89	1131558.0
90-91	1368162.0
92-93	1917602.0
94-95	5476157.0
96-97	2.9450844E7
98-99	2.18959195E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.225645063926002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.47255539393272	6.014038777898681
2	6.878267859861054	1.8193905873826446
3	4.428367225057139	1.7570403939398591
4	3.5728562408698776	1.8901331402471164
5	3.263148119458344	2.1578619409486834
6	2.9983584196732602	2.3793134539819576
7	2.8289722010891247	2.619048755912278
8	2.807800489331986	2.9707978145778076
9	2.5284403829779563	3.0096229563507224
>10	24.704265832929433	55.7986435617086
>50	0.34369481870517354	3.0420815078136028
>100	0.14273773458907493	3.5902740733250074
>500	0.015349299167052112	1.4100265602869442
>1k	0.012300138195234749	3.290693945137286
>5k	0.0014548345805850597	1.3358280238736224
>10k+	0.0014310095817611935	6.9152045066151455
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGTGAAGGTGTTAGTGGGAATGTTTGTGATGAGATTTTTAGTTGAAATA	1325885	0.48617694819034635	No Hit
TTGTGATGTTTTTTATTTTTTGGATGTTTTGTTTGTTTGTTAGGTTATGA	990209	0.36309090885756656	No Hit
TGGTGAGTAGGTTAAGGGTTAATAGTGTAATTGAATTATAGTGAAATTAT	852409	0.3125622555726816	No Hit
TTGTTTGTGGGTATTTTTATTATTATTGAGATTATTTGAGATTAATTGAG	519509	0.19049412292726642	No Hit
TGGTAGGAATTGTGATAATTATAGTGGTTGATGTAAAGTAAGTTTGTGTG	341046	0.12505511675034023	No Hit
TGGTTGTGTTTGGATATATGATTGTTATAGTTATTGAGGAATATTTAGAG	280460	0.10283937663482469	No Hit
TTGTTTAGTTGTTTATGTTAGTTTTTAATTAAGGAATAAGTGATTATGTT	273698	0.10035987914924856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.700302750236463E-6	0.0	0.0	1.5400605500472927E-5	0.0
2	7.700302750236463E-6	7.33362166689187E-7	0.0	1.5400605500472927E-5	0.0
3	8.43366491692565E-6	7.33362166689187E-7	0.0	1.5400605500472927E-5	0.0
4	1.0267070333648617E-5	7.33362166689187E-7	0.0	1.5400605500472927E-5	0.0
5	1.1733794667026992E-5	7.33362166689187E-7	0.0	1.576728658381752E-5	0.0
6	1.9800778500608047E-5	7.33362166689187E-7	0.0	1.576728658381752E-5	0.0
7	2.6767719084155325E-5	7.33362166689187E-7	0.0	1.576728658381752E-5	0.0
8	2.7867762334189105E-5	7.33362166689187E-7	0.0	1.576728658381752E-5	0.0
9	3.0434529917601262E-5	7.33362166689187E-7	0.0	1.576728658381752E-5	0.0
10-11	3.37346596677026E-5	7.33362166689187E-7	0.0	2.82344434175337E-5	0.0
12-13	4.5101773251385E-5	7.33362166689187E-7	0.0	2.82344434175337E-5	0.0
14-15	5.298541654329376E-5	7.33362166689187E-7	0.0	2.82344434175337E-5	0.0
16-17	5.8852313876807254E-5	7.33362166689187E-7	0.0	3.0801211000945854E-5	0.0
18-19	7.113613016885114E-5	7.33362166689187E-7	0.0	3.153457316763504E-5	0.0
20-21	9.368701679454363E-5	7.33362166689187E-7	0.0	3.153457316763504E-5	0.0
22-23	1.182546493786314E-4	7.33362166689187E-7	0.0	3.1717913709307334E-5	0.0
24-25	1.3328857379575973E-4	7.33362166689187E-7	0.0	3.1901254250979636E-5	0.0
26-27	1.4062219546265162E-4	7.33362166689187E-7	0.0	3.208459479265193E-5	0.0
28-29	1.4813915767121575E-4	7.33362166689187E-7	0.0	4.143496241793907E-5	0.0
30-31	1.5492275771309078E-4	7.33362166689187E-7	0.0	5.06019895015539E-5	0.0
32-33	1.6500648750506706E-4	7.33362166689187E-7	0.0	6.288580579359779E-5	0.0
34-35	1.7930704975550622E-4	7.33362166689187E-7	0.0	7.223617341888492E-5	0.0
36-37	1.9929116879778655E-4	7.33362166689187E-7	0.0	7.278619504390181E-5	0.0
38-39	2.1120830400648584E-4	7.33362166689187E-7	0.0	7.773638966905382E-5	0.0
40-41	2.200086500067561E-4	7.33362166689187E-7	0.0	7.773638966905382E-5	0.0
42-43	2.2605888788194192E-4	7.33362166689187E-7	0.0	7.773638966905382E-5	0.0
44-45	2.2990903925706012E-4	9.167027083614837E-7	0.0	9.222029246116527E-5	0.0
46-47	2.3522591496555673E-4	1.1000432500337806E-6	0.0	1.0047061683641862E-4	0.0
48-49	2.3742600146562428E-4	1.1000432500337806E-6	0.0	1.175212872119422E-4	0.0
50-51	2.4090947175739794E-4	1.1000432500337806E-6	0.0	1.419055792543577E-4	0.0
52-53	2.4530964475753304E-4	1.2833837917060774E-6	0.0	1.6060631450493196E-4	0.0
54-55	2.4604300692422225E-4	1.466724333378374E-6	0.0	1.6133967667162114E-4	0.0
56-57	2.4677636909091146E-4	1.466724333378374E-6	0.0	1.6610653075510085E-4	0.0
58-59	2.4842643396596207E-4	1.466724333378374E-6	0.0	1.7142340646359746E-4	0.0
60-61	2.486097745076344E-4	1.466724333378374E-6	0.0	1.8260717950560756E-4	0.0
62-63	2.486097745076344E-4	1.466724333378374E-6	0.0	1.8627399033905348E-4	0.0
64-65	2.48976455590979E-4	1.6500648750506706E-6	0.0	2.0754149317303992E-4	0.0
66-67	2.495264772159959E-4	1.8334054167229674E-6	0.0	2.0974157967310747E-4	0.0
68-69	2.500764988410128E-4	2.0167459583952643E-6	0.0	2.1157498508983044E-4	0.0
70-71	2.500764988410128E-4	2.200086500067561E-6	0.0	2.141417526732426E-4	0.0
72-73	2.500764988410128E-4	2.200086500067561E-6	0.0	2.3394253117385065E-4	0.0
74-75	2.500764988410128E-4	2.200086500067561E-6	0.0	2.5080986100770193E-4	0.0
76-77	2.500764988410128E-4	2.200086500067561E-6	0.0	3.600808238443908E-4	0.0
78-79	2.500764988410128E-4	2.200086500067561E-6	0.0	3.674144455112827E-4	0.0
80-81	2.500764988410128E-4	2.200086500067561E-6	0.0	3.716312779697455E-4	0.0
82-83	2.500764988410128E-4	2.200086500067561E-6	0.0	3.7474806717817453E-4	0.0
84-85	2.500764988410128E-4	2.200086500067561E-6	0.0	3.96015570012161E-4	0.0
86-87	2.500764988410128E-4	2.200086500067561E-6	0.0	3.9711561326219474E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGAC	14995	0.0	84.923164	1
CCCCCGG	10405	0.0	82.212944	1
CCGCCGG	5395	0.0	78.483765	1
CACGACG	171400	0.0	75.19809	2
ACGCGAC	4830	0.0	74.00499	1
GCGACGC	4345	0.0	73.70052	3
ACGACGC	181670	0.0	73.28981	3
CGCGACG	4465	0.0	71.41551	2
ACACGAC	188615	0.0	71.17043	1
CGTGTCC	7495	0.0	70.501755	9
GGGTACC	8130	0.0	70.42352	1
CCTCGTG	7665	0.0	70.298004	6
CCCTCGG	3490	0.0	69.40185	1
GCCGGTC	19680	0.0	66.60187	3
CGCCGGT	9885	0.0	66.57209	2
CGCCCGG	2930	0.0	66.009705	1
CTATGGC	3935	0.0	65.19561	4
GGTACCT	8725	0.0	64.24149	2
GGGCCGG	5065	0.0	62.720215	1
GGACTAT	13545	0.0	60.01832	1
>>END_MODULE