##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248456_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272716550
Sequences flagged as poor quality	0
Sequence length	20-99
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.194857613151825	33.0	31.0	34.0	31.0	34.0
2	31.833644305781956	31.0	31.0	34.0	31.0	34.0
3	32.496090603962244	33.0	31.0	34.0	31.0	34.0
4	35.94494788086752	37.0	35.0	37.0	35.0	37.0
5	35.761058872297994	37.0	35.0	37.0	35.0	37.0
6	35.73086009264931	37.0	35.0	37.0	35.0	37.0
7	35.717904949296255	37.0	35.0	37.0	35.0	37.0
8	35.67084135157914	37.0	35.0	37.0	35.0	37.0
9	37.13806763835932	38.0	37.0	39.0	35.0	39.0
10-11	37.136867414170496	38.0	37.0	39.0	35.0	39.0
12-13	37.19735214456182	38.0	37.0	39.0	35.0	39.0
14-15	38.3350335449022	39.0	38.0	40.0	35.0	41.0
16-17	38.28461217150188	39.0	38.0	40.0	35.0	41.0
18-19	38.30513087856237	39.0	38.0	40.0	35.0	41.0
20-21	38.28732048449029	39.0	38.0	40.0	35.0	41.0
22-23	38.22584327640178	39.0	38.0	40.0	34.5	41.0
24-25	38.13867613330724	39.0	38.0	40.0	34.0	41.0
26-27	38.019016998049274	39.0	37.5	40.0	34.0	41.0
28-29	37.84845871449909	39.0	37.0	40.0	34.0	41.0
30-31	37.74001691033975	39.0	37.0	40.0	34.0	41.0
32-33	37.62700367487271	39.0	37.0	40.0	33.5	41.0
34-35	37.46288820874883	39.0	37.0	40.0	33.0	41.0
36-37	37.28611521294091	38.0	36.5	40.0	33.0	41.0
38-39	37.13262123673857	38.0	36.0	40.0	32.0	41.0
40-41	37.210727613954404	38.5	36.5	40.0	32.5	41.0
42-43	37.43340137752127	39.0	37.0	40.0	33.0	41.0
44-45	37.36634550944832	39.0	37.0	40.0	33.0	41.0
46-47	37.27504774877451	39.0	36.5	40.0	33.0	41.0
48-49	37.17483520051892	39.0	36.0	40.0	32.0	41.0
50-51	37.015750740909255	39.0	36.0	40.0	32.0	41.0
52-53	36.85885194718415	38.0	36.0	40.0	31.0	41.0
54-55	36.66494119734321	38.0	35.5	40.0	31.0	41.0
56-57	36.44242855440042	38.0	35.0	40.0	31.0	41.0
58-59	36.19194112095867	38.0	35.0	40.0	30.0	41.0
60-61	36.03040135039832	38.0	34.5	40.0	30.0	40.0
62-63	36.007980589642045	38.0	34.5	40.0	30.0	40.0
64-65	35.767106263525406	37.5	34.0	39.5	30.0	40.0
66-67	35.5041972790919	37.0	34.0	39.0	29.0	40.0
68-69	35.21653083096837	36.5	34.0	39.0	29.0	40.0
70-71	34.89473207845075	36.0	34.0	39.0	29.0	40.0
72-73	34.5612616898094	36.0	33.5	38.0	28.5	40.0
74-75	34.233697481581395	35.0	33.0	38.0	28.0	39.5
76-77	32.20490083565202	33.5	30.5	36.0	25.5	38.5
78-79	33.21560451777634	34.5	32.0	36.5	26.0	39.0
80-81	33.27951157309595	35.0	32.0	36.0	26.0	39.0
82-83	33.01384408946018	35.0	32.0	36.0	26.0	38.0
84-85	32.77778023996536	35.0	32.0	36.0	26.0	37.0
86-87	32.50223216823798	34.0	32.0	35.0	26.0	37.0
88-89	32.251045459690744	34.0	32.0	35.0	25.5	36.5
90-91	32.10314359455347	34.0	31.5	35.0	25.0	36.0
92-93	31.935396033433165	34.0	31.0	35.0	25.0	36.0
94-95	31.828425991462424	34.0	31.0	35.0	25.0	35.0
96-97	31.817717694508286	34.0	31.0	35.0	25.0	35.0
98-99	32.208166222295525	34.0	31.5	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	11.0
10	45.0
11	147.0
12	447.0
13	960.0
14	1886.0
15	3706.0
16	7307.0
17	14929.0
18	29360.0
19	57008.0
20	105061.0
21	180849.0
22	297371.0
23	473075.0
24	734361.0
25	1112074.0
26	1631355.0
27	2307269.0
28	3165770.0
29	4232179.0
30	5598854.0
31	7444046.0
32	1.0101275E7
33	1.4274833E7
34	2.1508841E7
35	3.5250995E7
36	5.9337488E7
37	7.2002656E7
38	3.1303973E7
39	1538409.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.826480154500054	56.134315534681946	21.448159574634598	13.591044736183408
2	4.127120557519612	62.24040496612382	17.64167246245388	15.990802013902695
3	7.203551006339364	49.939489235525755	18.741254713985462	24.11570504414941
4	4.1537524030812	31.16747182011811	19.849260410105522	44.82951536669517
5	2.0481643750173606	52.67408417219605	22.965641807105072	22.312109645681506
6	1.7943612281276013	52.010874308954655	25.260017958279523	20.934746504638216
7	1.6860004161123332	50.16976708489843	25.690312686343926	22.453919812645314
8	1.8113614242939038	50.68905425467075	25.469881506853554	22.029702814181793
9	1.8012049573126565	49.88398118810164	25.4900061088702	22.824807745715496
10-11	1.83838933793392	48.649417004156305	26.14044428052332	23.37174937738646
12-13	1.9757348793096714	47.320962735851566	27.527250179719566	23.1760522051192
14-15	1.9902679173669513	47.650383337571554	27.028047252724484	23.331301492337005
16-17	2.1157804250375847	47.12330527768408	26.706289626012946	24.05462467126539
18-19	2.1025454622738797	46.67003965996807	27.083252784458484	24.144162093299563
20-21	2.2372110426201313	47.36806983995868	26.87193957105485	23.52277954636634
22-23	2.255199699801843	46.84775208905992	26.898187658019566	23.99886055311867
24-25	2.3431444893767353	46.8797482871113	27.2311577655722	23.54594945793977
26-27	2.365915139696475	46.916519315724855	27.16073855469122	23.556826989887448
28-29	2.4868144033452375	46.73914360786164	27.503030487589857	23.271011501203258
30-31	2.509715655565962	46.619213172290415	28.02062942430869	22.85044174783493
32-33	2.5494364362878494	46.49367114882199	27.273607585977473	23.68328482891269
34-35	2.590939234560762	47.35525664253524	27.58335958914045	22.47044453376355
36-37	2.6632889423979593	46.697074224947656	28.10575003149586	22.53388680115853
38-39	2.6692304955654516	46.69036824878063	27.943005359189655	22.69739589646426
40-41	2.716887925071215	46.60701790991255	27.744929945102665	22.931164219913576
42-43	2.8215477685700816	46.65252065811793	27.78585098928586	22.74008058402613
44-45	2.86304252628537	47.43781059651542	27.649547112455934	22.049599764743277
46-47	2.893535274374874	46.943030744255395	27.33521616656855	22.82821781480118
48-49	2.8592899564847984	46.60477440086084	28.64398418837963	21.89195145427473
50-51	2.940862718489689	46.96596744489278	27.803755049533997	22.28941478708353
52-53	2.935904614723733	47.18212759404961	27.546674175044455	22.335293616182202
54-55	2.9912072352440653	46.88326947418959	27.676361797195582	22.449161493370763
56-57	3.0739165190240114	46.461977025656886	28.102751555258276	22.361354900060824
58-59	3.0689670372955855	47.09575885161638	27.698592618874102	22.13668149221393
60-61	3.1477066791418102	47.08498376867446	27.726920356196022	22.040389195987707
62-63	3.1481413047335316	47.07727238077824	27.67315349600592	22.101432818482312
64-65	3.124291188273391	46.73178075943679	27.931349657212262	22.21257839507756
66-67	3.141934300185964	46.217772461075825	28.364357133821684	22.275936104916532
68-69	3.2140683999141837	46.045770144782225	28.416769311551466	22.32339214375213
70-71	3.1800710622132944	47.15537061751601	28.089706144713478	21.574852175557215
72-73	3.19797180155146	46.64762512129204	28.111028603418653	22.043374473737853
74-75	3.236382682587169	46.851170390696225	28.038949808517934	21.87349711819867
76-77	3.177851217987504	46.68752777308331	27.98537981013541	22.14924119879378
78-79	3.221377532068035	46.69862872284087	28.406530761039605	21.673462984051486
80-81	3.20673690446738	46.80690591558544	28.002438521207214	21.983918658739967
82-83	3.2502744349026993	46.87323326395547	27.892091127227225	21.984401173914602
84-85	3.2587169749496105	46.95313631650414	27.933565990634087	21.85458071791216
86-87	3.3038503404829855	47.32116450735185	28.029528905717566	21.34545624644759
88-89	3.2661698433378175	46.71354707972645	28.436101012658018	21.584182064277712
90-91	3.2511030682278994	46.92552500268586	28.37225083462471	21.451121094461527
92-93	3.2560143882999584	47.07501229341964	27.93063449417869	21.73833882410171
94-95	3.260481962511804	47.043829218885946	28.161923487870876	21.533765330731374
96-97	3.0771897234867507	46.807404526305845	28.782184584140115	21.333221166067297
98-99	2.7261277039240026	45.00037654518522	29.40413984345455	22.86935590743623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188905.0
1	121608.5
2	59314.5
3	72163.5
4	93177.5
5	127216.5
6	188451.0
7	279480.5
8	390279.5
9	541394.5
10	792657.5
11	1209453.0
12	1806523.0
13	2555987.5
14	3521085.0
15	4743321.5
16	6141236.0
17	7670051.0
18	9387060.0
19	1.12506085E7
20	1.28877645E7
21	1.44340175E7
22	1.58875555E7
23	1.6835406E7
24	1.73200245E7
25	1.74601455E7
26	1.7162208E7
27	1.6415018E7
28	1.53280155E7
29	1.3970152E7
30	1.25256885E7
31	1.097188E7
32	9231541.5
33	7550677.0
34	6120492.0
35	4954886.0
36	4053232.0
37	3336879.0
38	2762262.5
39	2238556.0
40	1769716.0
41	1430419.5
42	1169278.0
43	929879.0
44	723379.5
45	568289.5
46	453875.5
47	363204.0
48	293207.5
49	239580.5
50	199996.5
51	165437.5
52	132705.0
53	110666.5
54	97344.5
55	89595.5
56	88993.5
57	96100.0
58	105356.5
59	116878.0
60	129485.5
61	135714.0
62	132572.0
63	120029.0
64	101518.5
65	82045.0
66	64025.0
67	48438.5
68	37323.5
69	28760.0
70	20858.0
71	16332.0
72	13667.0
73	10105.5
74	6820.5
75	4865.0
76	3806.5
77	3135.5
78	2497.0
79	1858.5
80	1389.0
81	1100.5
82	926.0
83	742.5
84	573.5
85	472.0
86	406.0
87	361.0
88	307.0
89	259.5
90	238.0
91	218.0
92	207.0
93	214.0
94	212.0
95	246.5
96	294.0
97	323.0
98	501.0
99	1056.5
100	1456.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015193064007299887
2	0.02101045939456186
3	0.011131337647091824
4	2.200086500067561E-6
5	0.0013108848729569216
6	7.33362166689187E-7
7	6.343582741861468E-4
8	0.002234554521901953
9	3.666810833445935E-7
10-11	9.53370816695943E-6
12-13	0.0
14-15	0.0
16-17	0.007034226562341009
18-19	0.001568661674548171
20-21	2.530236008607115E-5
22-23	0.0
24-25	3.320731981061553E-5
26-27	4.693109830349549E-4
28-29	4.3886293141146576E-5
30-31	1.5817414524772421E-4
32-33	8.163372791087321E-4
34-35	0.008718430503273622
36-37	0.019865069834913547
38-39	0.023140828022428107
40-41	0.026775867335627333
42-43	0.020066949365640477
44-45	0.005660697691744042
46-47	0.0035193655788516073
48-49	0.0017257783508632584
50-51	4.113147436247436E-4
52-53	0.006473125561258843
54-55	0.004262375070735459
56-57	0.0037411294276241706
58-59	0.02263277663072334
60-61	0.05846594215767992
62-63	0.04553582095980359
64-65	0.01774632144962918
66-67	0.007416596505281107
68-69	0.005794821091171477
70-71	0.005400838207839116
72-73	0.0036104528996538637
74-75	0.002419539499859703
76-77	0.004310015272870289
78-79	0.012572591879002419
80-81	0.016925077990515364
82-83	0.02728619420053389
84-85	0.006377338204404991
86-87	2.9659749110513774E-4
88-89	1.4252361649414572E-4
90-91	1.6493961148474515E-4
92-93	9.706117074513429E-4
94-95	0.006602368308556965
96-97	0.00714228851839073
98-99	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	68910.0
22-23	92106.0
24-25	104144.0
26-27	126935.0
28-29	122281.0
30-31	151307.0
32-33	179405.0
34-35	206714.0
36-37	185536.0
38-39	184967.0
40-41	197932.0
42-43	201770.0
44-45	218592.0
46-47	238440.0
48-49	261728.0
50-51	269331.0
52-53	275885.0
54-55	305760.0
56-57	306628.0
58-59	325280.0
60-61	363185.0
62-63	380277.0
64-65	393224.0
66-67	415383.0
68-69	444874.0
70-71	480371.0
72-73	518693.0
74-75	518664.0
76-77	530950.0
78-79	575378.0
80-81	642694.0
82-83	720934.0
84-85	847394.0
86-87	943741.0
88-89	1105030.0
90-91	1423220.0
92-93	2080218.0
94-95	4351904.0
96-97	1.8569359E7
98-99	2.33387406E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.693730029015915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.00915978135018	4.210813930548068
2	7.550415653370409	1.7658504451473878
3	5.218744636535623	1.830797726100671
4	4.31145431305309	2.0166793107711642
5	3.8035211305637766	2.22386746302351
6	3.586088369523465	2.5160849552040765
7	3.4399474107368646	2.815807143461846
8	3.3067720742234283	3.09347999227662
9	3.0760073227480613	3.2372999279542674
>10	29.259827404931944	57.18883345397627
>50	0.23372736119000215	1.8338398649004
>100	0.15482610540324113	3.639960558463283
>500	0.02333227686268471	1.901382936342832
>1k	0.021602757827906213	5.224025999459168
>5k	0.0027884666736722324	2.2265488287456194
>10k+	0.001784935005489654	4.2747274636246155
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0267070333648617E-5	0.0	0.0	1.466724333378374E-6	0.0
2	1.3200519000405367E-5	0.0	0.0	7.33362166689187E-6	0.0
3	1.3200519000405367E-5	0.0	0.0	1.1037100608672264E-4	0.0
4	1.466724333378374E-5	0.0	0.0	1.188046710036483E-4	0.0
5	1.6500648750506706E-5	0.0	0.0	1.1953803317033748E-4	0.0
6	1.7234010917195894E-5	0.0	0.0	1.228381629204388E-4	0.0
7	1.760069200054049E-5	0.0	0.0	1.2320484400378342E-4	0.0
8	1.796737308388508E-5	0.0	0.0	1.3677204408753339E-4	0.0
9	1.796737308388508E-5	0.0	0.0	1.6537316858841167E-4	0.0
10-11	1.9984119042280343E-5	0.0	0.0	1.697733415885468E-4	0.0
12-13	2.383427041739858E-5	0.0	0.0	1.7600692000540487E-4	0.0
14-15	2.9517827209239775E-5	0.0	0.0	1.9984119042280345E-4	0.0
16-17	3.6668108334459346E-5	0.0	0.0	2.0497472558962776E-4	0.0
18-19	5.005196787653701E-5	0.0	0.0	3.3166303988518483E-4	0.0
20-21	7.3519557210591E-5	0.0	0.0	4.1801643501283663E-4	0.0
22-23	9.515374112792201E-5	0.0	0.0	5.190370734742721E-4	0.0
24-25	1.2357152508712802E-4	0.0	0.0	5.929233117682077E-4	0.0
26-27	1.356720008374996E-4	0.0	0.0	5.96223441518309E-4	0.0
28-29	1.5492275771309075E-4	0.0	0.0	6.361916796028698E-4	0.0
30-31	1.7784032542212785E-4	0.0	0.0	6.796433879792041E-4	0.0
32-33	2.0992492021477976E-4	0.0	0.0	9.165193678198115E-4	0.0
34-35	2.662104665081749E-4	0.0	0.0	0.0011612789909523275	0.0
36-37	3.149790505930058E-4	0.0	0.0	0.0012247148183709422	0.0
38-39	3.536639048858604E-4	0.0	0.0	0.0013706538895420905	0.0
40-41	3.6686442388626577E-4	0.0	0.0	0.0014795581712954348	0.0
42-43	3.866652023868739E-4	0.0	0.0	0.0015967127774240324	0.0
44-45	4.04082553845742E-4	0.0	0.0	0.0018196548760975452	0.0
46-47	4.174664133878197E-4	0.0	0.0	0.0018557729628069877	0.0
48-49	4.3195031617993115E-4	0.0	0.0	0.0019589936877684906	0.0
50-51	4.475342622220764E-4	0.0	0.0	0.0029033808179224916	0.0
52-53	4.6623499747265064E-4	0.0	0.0	0.00392110416474541	0.0
54-55	4.880525219316539E-4	0.0	0.0	0.004185664566378535	0.0
56-57	5.100533869323296E-4	0.0	0.0	0.004572146428223737	0.0
58-59	5.357210627664512E-4	0.0	0.0	0.006559191218868088	0.0
60-61	5.69639062975826E-4	0.0	0.0	0.006740698355123663	0.0
62-63	6.099739821437313E-4	0.0	0.0	0.006912855123753949	0.0
64-65	6.473754526448799E-4	0.0	0.0	0.007574347798107596	0.0
66-67	6.869770096460958E-4	0.0	0.0	0.007858525637699655	0.0
68-69	7.214450314804877E-4	0.0	0.0	0.008072484049831226	0.0
70-71	7.34645550480893E-4	0.0	0.0	0.008425231252008725	0.0
72-73	7.417958316061127E-4	0.0	0.0	0.009172527299865008	0.0
74-75	7.425291937728019E-4	0.0	0.0	0.009380802155204735	0.0
76-77	7.425291937728019E-4	0.0	0.0	0.011691076320817347	0.0
78-79	7.425291937728019E-4	0.0	0.0	0.011891834213948512	0.0
80-81	7.425291937728019E-4	0.0	0.0	0.011957470127867194	0.0
82-83	7.425291937728019E-4	0.0	0.0	0.012068757836662277	0.0
84-85	7.425291937728019E-4	0.0	0.0	0.01334609139049317	0.0
86-87	7.425291937728019E-4	0.0	0.0	0.013447112028954605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCCGG	545	0.0	49.120087	1
GGGTACC	975	0.0	47.002438	1
TAGCCAC	11055	0.0	46.87173	1
GGCCGGT	1215	0.0	46.301617	2
GGCCATA	3745	0.0	46.282326	1
GTCGGTC	5250	0.0	46.222984	3
GCCGGTC	2450	0.0	45.914417	3
GGCCATT	2140	0.0	45.79769	1
GGACCAC	2570	0.0	45.19709	1
AAGCCAT	32710	0.0	43.931004	1
GGCCAAA	7395	0.0	42.827316	1
TGACGGG	990	0.0	42.62372	1
GGCCCAT	2165	0.0	41.496445	1
TGGCAAC	2485	0.0	40.90018	1
TGCCGTC	4010	0.0	40.717068	5
GGGCAAG	1135	0.0	40.37654	1
GGACAAT	4020	0.0	39.84302	1
AGCCATA	34480	0.0	39.47342	2
GGGTCGG	2140	0.0	39.4369	1
TGGCCAC	1450	0.0	38.17644	1
>>END_MODULE