##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248455_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15544734 Sequences flagged as poor quality 0 Sequence length 100 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441165863629447 33.0 31.0 34.0 28.0 34.0 2 31.668725241615586 34.0 31.0 34.0 28.0 34.0 3 31.75169655524501 34.0 31.0 34.0 28.0 34.0 4 35.01580927663349 37.0 35.0 37.0 32.0 37.0 5 34.9040921510783 37.0 35.0 37.0 32.0 37.0 6 34.715422341739654 37.0 35.0 37.0 31.0 37.0 7 34.76654647162184 37.0 35.0 37.0 32.0 37.0 8 34.8836503731746 37.0 35.0 37.0 32.0 37.0 9 36.42258407252257 39.0 37.0 39.0 32.0 39.0 10-11 36.5303098142432 39.0 37.0 39.0 32.0 39.0 12-13 36.567766646891485 39.0 37.0 39.0 32.0 39.0 14-15 37.77410752091352 40.0 38.0 41.0 32.0 41.0 16-17 37.675587983686306 40.0 38.0 41.0 32.0 41.0 18-19 37.64420597354705 40.0 38.0 41.0 32.0 41.0 20-21 37.56487100390396 40.0 38.0 41.0 31.5 41.0 22-23 37.49379503695592 40.0 38.0 41.0 31.0 41.0 24-25 37.43807163248982 40.0 38.0 41.0 31.0 41.0 26-27 37.32333772967746 40.0 38.0 41.0 30.5 41.0 28-29 37.189808619433435 40.0 37.0 41.0 30.0 41.0 30-31 37.12388590888722 40.0 37.0 41.0 30.0 41.0 32-33 37.031429293032616 40.0 37.0 41.0 30.0 41.0 34-35 36.91556629402601 40.0 37.0 41.0 30.0 41.0 36-37 36.78312459383351 40.0 37.0 41.0 30.0 41.0 38-39 36.63540302458698 39.0 37.0 41.0 29.0 41.0 40-41 36.44548845287414 39.0 36.0 41.0 28.0 41.0 42-43 36.238671565560395 39.0 35.5 41.0 28.0 41.0 44-45 35.99835085630928 39.0 35.0 40.0 28.0 41.0 46-47 35.746079958653524 39.0 35.0 40.0 27.0 41.0 48-49 35.413692122361184 38.0 34.0 40.0 26.5 41.0 50-51 35.02818423911275 37.5 33.5 40.0 26.0 41.0 52-53 34.63337349484398 37.0 33.0 40.0 26.0 41.0 54-55 34.2520566450349 36.0 33.0 39.5 26.0 41.0 56-57 33.85693351201763 35.5 32.0 39.0 25.0 41.0 58-59 33.91203683511085 35.0 33.0 39.0 26.0 41.0 60-61 33.679550290149706 35.0 32.5 39.0 26.0 41.0 62-63 33.402096362665326 35.0 32.0 38.0 25.5 40.5 64-65 33.13910424584943 35.0 32.0 37.0 25.0 40.0 66-67 32.901572616166995 35.0 32.0 37.0 25.0 40.0 68-69 32.68444644983954 35.0 32.0 36.0 24.0 39.5 70-71 32.48055473319775 35.0 31.0 36.0 24.0 39.0 72-73 32.47984745187663 35.0 32.0 35.5 24.0 39.0 74-75 32.5688122743046 35.0 32.0 35.0 25.0 39.0 76-77 32.427430440430825 35.0 32.0 35.0 24.0 38.0 78-79 32.18781768797073 35.0 32.0 35.0 24.0 37.0 80-81 31.935347012049228 35.0 32.0 35.0 23.0 37.0 82-83 31.666654122225573 35.0 31.5 35.0 22.5 36.0 84-85 31.397418154598206 35.0 31.0 35.0 20.0 36.0 86-87 31.116405819488453 35.0 31.0 35.0 20.0 36.0 88-89 30.81424162677856 35.0 31.0 35.0 18.5 36.0 90-91 30.49288826685616 35.0 30.0 35.0 16.0 36.0 92-93 30.176193815860728 34.0 30.0 35.0 13.5 35.0 94-95 29.83871232534439 34.0 29.0 35.0 10.0 35.0 96-97 29.50185609480355 34.0 29.0 35.0 10.0 35.0 98-99 29.156396983055483 34.0 27.5 35.0 8.0 35.0 100 28.05199323449343 33.0 25.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3150743048985909 1101 2 0.3241968112072371 1101 3 0.33627404595126364 1101 4 0.3060669199214132 1101 5 0.16033953074725815 1101 6 0.2565673093092329 1101 7 0.2449405615497824 1101 8 0.29751677023266865 1101 9 0.406043105353632 1101 10-11 0.4480301125021384 1101 12-13 0.44493647098430245 1101 14-15 0.5711232044467209 1101 16-17 0.5763860567244237 1101 18-19 0.6024116108590931 1101 20-21 0.6624729909884408 1101 22-23 0.6138300861480772 1101 24-25 0.6493192754806287 1101 26-27 0.6433537158282476 1101 28-29 0.6911746105198162 1101 30-31 0.6990979224493401 1101 32-33 0.7115321651275792 1101 34-35 0.7235754187870427 1101 36-37 0.790275477798879 1101 38-39 0.7463299067409253 1101 40-41 0.7204383530477472 1101 42-43 0.7555429403315514 1101 44-45 0.7262805754405619 1101 46-47 0.7245902877282546 1101 48-49 0.7304266243379516 1101 50-51 0.7605822110232126 1101 52-53 0.679943768646325 1101 54-55 0.6818011664184453 1101 56-57 0.6437150602970476 1101 58-59 0.6460172107257947 1101 60-61 0.613700153005972 1101 62-63 0.5865368113890028 1101 64-65 0.6104426729515637 1101 66-67 0.5953553951069566 1101 68-69 0.5882615364950823 1101 70-71 0.551486627143916 1101 72-73 0.5613048407496919 1101 74-75 0.5466583523711535 1101 76-77 0.5181744547456262 1101 78-79 0.5354255962057479 1101 80-81 0.5206680765576088 1101 82-83 0.5251662360875464 1101 84-85 0.5340757724545853 1101 86-87 0.5650011556058097 1101 88-89 0.5737681405559556 1101 90-91 0.5342361575641341 1101 92-93 0.5551255446706911 1101 94-95 0.5483863666929274 1101 96-97 0.4740446661363471 1101 98-99 0.5186399667395065 1101 100 0.5671407900225489 1102 1 0.20580413018224775 1102 2 0.22051341721513396 1102 3 0.27922214715626836 1102 4 0.22390513234853415 1102 5 0.1996375478576482 1102 6 0.016738403514075628 1102 7 0.11020938877863529 1102 8 0.19065832695894613 1102 9 0.2842622352301376 1102 10-11 0.29049645606311003 1102 12-13 0.3053103076675612 1102 14-15 0.4658240406847298 1102 16-17 0.4972298566909856 1102 18-19 0.5219275968563437 1102 20-21 0.5273288703012255 1102 22-23 0.5333155083092223 1102 24-25 0.46975128417496137 1102 26-27 0.5088283312094646 1102 28-29 0.5112456196259174 1102 30-31 0.5553277767626454 1102 32-33 0.5539104145954923 1102 34-35 0.5514829350835058 1102 36-37 0.5204508423472376 1102 38-39 0.5223102138808144 1102 40-41 0.5203488883756009 1102 42-43 0.5020642010737575 1102 44-45 0.4529315659444606 1102 46-47 0.480494829380369 1102 48-49 0.48722999980352455 1102 50-51 0.4613362813889026 1102 52-53 0.46808215869234715 1102 54-55 0.4407535897552961 1102 56-57 0.38941796326302835 1102 58-59 0.37533237367469496 1102 60-61 0.398701390501337 1102 62-63 0.382208764454802 1102 64-65 0.38633460857933954 1102 66-67 0.39441001141110377 1102 68-69 0.39884147513680546 1102 70-71 0.36809736664857695 1102 72-73 0.39520004570019296 1102 74-75 0.38887295557410795 1102 76-77 0.36640796463153436 1102 78-79 0.3543056273154974 1102 80-81 0.4230986975270632 1102 82-83 0.3933505270876587 1102 84-85 0.361315786286621 1102 86-87 0.32625283051357457 1102 88-89 0.2597131955554026 1102 90-91 0.3039194293060561 1102 92-93 0.2242495130505766 1102 94-95 0.26366250952633763 1102 96-97 0.28415891369635204 1102 98-99 0.23094594263823964 1102 100 0.1872340517484261 1103 1 0.04293430970428602 1103 2 0.08375274695878332 1103 3 0.11280185360216066 1103 4 0.04112806300433647 1103 5 0.01908949693950035 1103 6 -0.07719757180881714 1103 7 -0.024528488098347623 1103 8 0.06566267619508892 1103 9 0.06097952850836208 1103 10-11 0.11807554983389679 1103 12-13 0.1717141939677873 1103 14-15 0.26973593241388016 1103 16-17 0.3321554131550286 1103 18-19 0.2768220591435693 1103 20-21 0.3270252513738896 1103 22-23 0.31285439308875596 1103 24-25 0.3057276484851599 1103 26-27 0.28655188416532695 1103 28-29 0.3358237628173981 1103 30-31 0.30253768481488663 1103 32-33 0.33886242642986986 1103 34-35 0.3442713862816973 1103 36-37 0.3454057871858467 1103 38-39 0.2963764741275554 1103 40-41 0.32406978478793036 1103 42-43 0.30720186969699625 1103 44-45 0.2593686701710709 1103 46-47 0.3367889562987898 1103 48-49 0.31889025021320805 1103 50-51 0.33108118363147554 1103 52-53 0.306415912338025 1103 54-55 0.3030063472689122 1103 56-57 0.29783030108184505 1103 58-59 0.285915357749424 1103 60-61 0.27692494201520645 1103 62-63 0.29149819692020174 1103 64-65 0.3245693477077083 1103 66-67 0.26030162519976585 1103 68-69 0.23625279547187716 1103 70-71 0.26145068417554995 1103 72-73 0.29679892752665893 1103 74-75 0.28804222651047695 1103 76-77 0.27890300140157365 1103 78-79 0.24001684529251577 1103 80-81 0.22933137647428836 1103 82-83 0.23813950205318335 1103 84-85 0.23421766769081387 1103 86-87 0.19401868979754866 1103 88-89 0.23319627294317513 1103 90-91 0.24775127787811613 1103 92-93 0.26337903434368926 1103 94-95 0.22053843557398878 1103 96-97 0.2599744520109901 1103 98-99 0.26367043786715527 1103 100 0.2541530544100823 1104 1 0.12615920260287794 1104 2 0.1576430234778421 1104 3 0.229925021466876 1104 4 0.18690261343529357 1104 5 0.1288693811451651 1104 6 0.00433157614623525 1104 7 0.06929164648179409 1104 8 0.13608162269506607 1104 9 0.1710036468097087 1104 10-11 0.21903769627470382 1104 12-13 0.2604934624966404 1104 14-15 0.3129109056217061 1104 16-17 0.3688542566355437 1104 18-19 0.344260366766612 1104 20-21 0.32947823389915953 1104 22-23 0.33904304459968415 1104 24-25 0.3753877415371818 1104 26-27 0.37390595073575383 1104 28-29 0.34932606926446397 1104 30-31 0.356342735011431 1104 32-33 0.4081082517770511 1104 34-35 0.33930747585963417 1104 36-37 0.36690167947913466 1104 38-39 0.38498150484473825 1104 40-41 0.4041143749342879 1104 42-43 0.4054521943102216 1104 44-45 0.37985269908768515 1104 46-47 0.4022218910940012 1104 48-49 0.42591698772024245 1104 50-51 0.3773812080868808 1104 52-53 0.35448233843821697 1104 54-55 0.3620042979193343 1104 56-57 0.36234018137079715 1104 58-59 0.3524199307166711 1104 60-61 0.32760347147907964 1104 62-63 0.29430576336088876 1104 64-65 0.29883879427312365 1104 66-67 0.32290437483127477 1104 68-69 0.2932475134094261 1104 70-71 0.2998520969110601 1104 72-73 0.2774447524480905 1104 74-75 0.27778533178516085 1104 76-77 0.3041009045046792 1104 78-79 0.30161625206444853 1104 80-81 0.2806496834348273 1104 82-83 0.2610171403967847 1104 84-85 0.2217598179132061 1104 86-87 0.20703424491112088 1104 88-89 0.1949582862402366 1104 90-91 0.19838861500827676 1104 92-93 0.1997859595614493 1104 94-95 0.22802040344868857 1104 96-97 0.2638491392336384 1104 98-99 0.23356914329822587 1104 100 0.20726707759759222 1105 1 0.17936560635681076 1105 2 0.19723953947764272 1105 3 0.19468577876073567 1105 4 0.20354859612559295 1105 5 0.15227544084616795 1105 6 0.1192371370000842 1105 7 0.08749385304041368 1105 8 0.14040786680761386 1105 9 0.16110676190361062 1105 10-11 0.17414414952521895 1105 12-13 0.20493642136215584 1105 14-15 0.2848661741943914 1105 16-17 0.2915460897765172 1105 18-19 0.31694388400127593 1105 20-21 0.3021867910611107 1105 22-23 0.27654749258026357 1105 24-25 0.28261922621894087 1105 26-27 0.3416485608692952 1105 28-29 0.330703879415708 1105 30-31 0.29748577155925915 1105 32-33 0.3042779577666579 1105 34-35 0.32714244573387674 1105 36-37 0.3227338510230382 1105 38-39 0.2823374733688411 1105 40-41 0.28451175945951945 1105 42-43 0.2859508493335312 1105 44-45 0.3469433297288802 1105 46-47 0.3738590258572714 1105 48-49 0.3399079296868308 1105 50-51 0.3528745860050222 1105 52-53 0.33831568904810183 1105 54-55 0.2860735136221493 1105 56-57 0.289650246006282 1105 58-59 0.26943353522202784 1105 60-61 0.2588104688832189 1105 62-63 0.2643431133169969 1105 64-65 0.2755672729842047 1105 66-67 0.25270283826279893 1105 68-69 0.27700808161183943 1105 70-71 0.268646671337585 1105 72-73 0.2689292129183869 1105 74-75 0.24287256667168577 1105 76-77 0.2786169082497949 1105 78-79 0.2759080994612688 1105 80-81 0.25447477003240593 1105 82-83 0.2343109417956981 1105 84-85 0.23067354954170938 1105 86-87 0.2074146343657297 1105 88-89 0.22562302565237147 1105 90-91 0.2715926998294904 1105 92-93 0.23183986919324084 1105 94-95 0.2565972070621889 1105 96-97 0.2974700653991569 1105 98-99 0.35210119412638363 1105 100 0.36296229186856266 1106 1 0.09295219218179085 1106 2 0.048000664027910034 1106 3 0.09652883972530191 1106 4 0.08421318558713153 1106 5 0.045025833470930365 1106 6 0.04118821430274977 1106 7 0.009461872211332434 1106 8 0.0708723339884827 1106 9 0.09064936097785647 1106 10-11 0.08030652362520385 1106 12-13 0.09903516835532855 1106 14-15 0.17261445798111197 1106 16-17 0.1953143858317432 1106 18-19 0.1915813917044744 1106 20-21 0.17365763918033394 1106 22-23 0.1703244209014798 1106 24-25 0.21091659797718876 1106 26-27 0.2049943861685506 1106 28-29 0.2216259088542074 1106 30-31 0.21843758189088192 1106 32-33 0.236706705181561 1106 34-35 0.24936091949474815 1106 36-37 0.24582116767330575 1106 38-39 0.22389517059937702 1106 40-41 0.2384065938838944 1106 42-43 0.2183898204987571 1106 44-45 0.2197740316612098 1106 46-47 0.23834594865559922 1106 48-49 0.24372012297179424 1106 50-51 0.23895857384100339 1106 52-53 0.20440740908806276 1106 54-55 0.20121756054279416 1106 56-57 0.1711625252823481 1106 58-59 0.1561993766472014 1106 60-61 0.12132224479575626 1106 62-63 0.18486275640238148 1106 64-65 0.21721391906643817 1106 66-67 0.13662302200807375 1106 68-69 0.17887799280936179 1106 70-71 0.21246828737428558 1106 72-73 0.17103358703786142 1106 74-75 0.19570885780317582 1106 76-77 0.1586999893116996 1106 78-79 0.20380680965136833 1106 80-81 0.16897574366418766 1106 82-83 0.1835605019439619 1106 84-85 0.16996260122528284 1106 86-87 0.15070465986303816 1106 88-89 0.14464139682396038 1106 90-91 0.1943976897474613 1106 92-93 0.22732826180983423 1106 94-95 0.182043282611847 1106 96-97 0.21166270107228868 1106 98-99 0.2531302165170999 1106 100 0.2456582153760678 1107 1 0.01755791787194738 1107 2 0.05111598849187615 1107 3 0.07897093286873869 1107 4 0.03517449982780363 1107 5 0.051467630250378704 1107 6 0.029922994971677497 1107 7 0.015862998226197078 1107 8 0.04861658175090611 1107 9 0.03343466908940229 1107 10-11 0.05490610194819112 1107 12-13 0.12285654827926606 1107 14-15 0.15553277289522072 1107 16-17 0.1320297498592211 1107 18-19 0.11771644372558399 1107 20-21 0.11913060049125335 1107 22-23 0.10348057972475999 1107 24-25 0.1064645039087182 1107 26-27 0.13089342386101777 1107 28-29 0.13600642615617176 1107 30-31 0.14351579499709288 1107 32-33 0.14730703679614976 1107 34-35 0.13793800092008723 1107 36-37 0.15001783889881892 1107 38-39 0.1372768873246244 1107 40-41 0.13466940372833136 1107 42-43 0.14030971536239178 1107 44-45 0.08552529028579414 1107 46-47 0.12118178420197978 1107 48-49 0.1191418396258257 1107 50-51 0.12173151853102127 1107 52-53 0.1602076151083338 1107 54-55 0.12868064120723233 1107 56-57 0.1596309434167864 1107 58-59 0.10959945237141966 1107 60-61 0.10580899546726386 1107 62-63 0.16343586725616888 1107 64-65 0.10796209524134781 1107 66-67 0.16356827236035798 1107 68-69 0.14893010121838302 1107 70-71 0.17453489238818776 1107 72-73 0.11821815482071685 1107 74-75 0.12321469166572996 1107 76-77 0.14933649055656417 1107 78-79 0.21155622371904315 1107 80-81 0.1079949054695355 1107 82-83 0.20785318196567815 1107 84-85 0.13056540740985767 1107 86-87 0.12154934948824447 1107 88-89 0.16248885057676077 1107 90-91 0.10630201922885618 1107 92-93 0.10708316129185746 1107 94-95 0.09398571147123036 1107 96-97 0.166761427960477 1107 98-99 0.2298287349235011 1107 100 0.21631990019679392 1108 1 0.1684678678145417 1108 2 0.13949495366703246 1108 3 0.12062688187299386 1108 4 0.13280662425014356 1108 5 0.14763165069808792 1108 6 0.16981653567276567 1108 7 0.08871259063307235 1108 8 0.09489717942916087 1108 9 0.11653246836681319 1108 10-11 0.13290717858031798 1108 12-13 0.12041486346858221 1108 14-15 0.11399860519969707 1108 16-17 0.10919265706377956 1108 18-19 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2215 6 -0.09333262536591036 2215 7 -0.13966636077892502 2215 8 -0.16505134611227135 2215 9 -0.27598287786268827 2215 10-11 -0.3000671702856934 2215 12-13 -0.3662825694739382 2215 14-15 -0.4783830207207984 2215 16-17 -0.543088588911921 2215 18-19 -0.5302949197661917 2215 20-21 -0.5335857718970658 2215 22-23 -0.5232926943951526 2215 24-25 -0.5517991941836868 2215 26-27 -0.5570124894925854 2215 28-29 -0.5665316076974989 2215 30-31 -0.5382708916535321 2215 32-33 -0.5251345594574133 2215 34-35 -0.5778012876537204 2215 36-37 -0.6017967453067428 2215 38-39 -0.5714849978570484 2215 40-41 -0.6121573798104407 2215 42-43 -0.5898119112413127 2215 44-45 -0.5535395425092489 2215 46-47 -0.5880352730703677 2215 48-49 -0.5250477654046719 2215 50-51 -0.5589075214607888 2215 52-53 -0.535511776577529 2215 54-55 -0.4845775215312713 2215 56-57 -0.4312237972515902 2215 58-59 -0.4248720567968647 2215 60-61 -0.42026949743759445 2215 62-63 -0.4231734583033102 2215 64-65 -0.5357264629666219 2215 66-67 -0.43673934243349777 2215 68-69 -0.4410196742016268 2215 70-71 -0.4325121070772866 2215 72-73 -0.4305335046253944 2215 74-75 -0.4600520233292613 2215 76-77 -0.44710563303750206 2215 78-79 -0.38871805845496965 2215 80-81 -0.4254304218131324 2215 82-83 -0.4642081371220321 2215 84-85 -0.42211820340716244 2215 86-87 -0.3858984003185526 2215 88-89 -0.40171018236787503 2215 90-91 -0.4178455272906447 2215 92-93 -0.40522446457869066 2215 94-95 -0.3938304626891451 2215 96-97 -0.3250231032251385 2215 98-99 -0.3555129197521474 2215 100 -0.3391744878854013 2216 1 -0.04631796719966985 2216 2 -0.07018286116778683 2216 3 -0.13557947184720476 2216 4 -0.09862623204687537 2216 5 -0.03459988831722427 2216 6 0.09144839695356666 2216 7 0.04621464513427043 2216 8 -0.07683427017428102 2216 9 -0.15777876520004241 2216 10-11 -0.23335388050218597 2216 12-13 -0.30562579818447233 2216 14-15 -0.3758126412272702 2216 16-17 -0.449396874547638 2216 18-19 -0.4515424790425584 2216 20-21 -0.48012652995983274 2216 22-23 -0.44297676943888575 2216 24-25 -0.4669071637095854 2216 26-27 -0.4401031215042508 2216 28-29 -0.42115771772127175 2216 30-31 -0.4401343567069844 2216 32-33 -0.4796289455042171 2216 34-35 -0.46717039670004823 2216 36-37 -0.43791677460698253 2216 38-39 -0.45704780273086953 2216 40-41 -0.49331538619271953 2216 42-43 -0.5195470768407944 2216 44-45 -0.5191697980082424 2216 46-47 -0.5591169209083233 2216 48-49 -0.4946374003301841 2216 50-51 -0.4745283491231689 2216 52-53 -0.4485785386009127 2216 54-55 -0.4428969403328793 2216 56-57 -0.46603866042086395 2216 58-59 -0.412766610603704 2216 60-61 -0.42876879612671104 2216 62-63 -0.41890723514549677 2216 64-65 -0.463601283017411 2216 66-67 -0.42318639981620976 2216 68-69 -0.4457031600055643 2216 70-71 -0.4520111377200706 2216 72-73 -0.42853294311453993 2216 74-75 -0.43224754233397533 2216 76-77 -0.41401366079416135 2216 78-79 -0.4489638638764397 2216 80-81 -0.5038941697016881 2216 82-83 -0.44671591855334825 2216 84-85 -0.40688702499690876 2216 86-87 -0.3956883074497419 2216 88-89 -0.38463191052729684 2216 90-91 -0.3955698601210038 2216 92-93 -0.5067330570812487 2216 94-95 -0.445438567393591 2216 96-97 -0.3960927005317352 2216 98-99 -0.40214595738724057 2216 100 -0.3053005020132922 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 16345.0 1 827.0 2 457.0 3 421.0 4 375.0 5 1566.0 6 8017.0 7 11970.0 8 13910.0 9 18263.0 10 29216.0 11 45439.0 12 55450.0 13 61280.0 14 64188.0 15 67607.0 16 69218.0 17 70023.0 18 71679.0 19 75575.0 20 80852.0 21 87150.0 22 94969.0 23 104906.0 24 118004.0 25 134083.0 26 155029.0 27 185561.0 28 228045.0 29 300438.0 30 402583.0 31 502368.0 32 631312.0 33 805594.0 34 1076495.0 35 1721054.0 36 3029971.0 37 2702686.0 38 1847410.0 39 648517.0 40 5881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.736735992566253 23.636144485483925 30.37857020179837 29.24854932015145 2 15.600629994299132 22.474885580023127 33.66595486329188 28.258529562385863 3 14.214338716128525 23.300164090834162 31.748667957723274 30.73682923531404 4 13.139056874295212 21.597662637722372 32.45119254715519 32.812087940827226 5 13.08549465102857 21.943040926330543 33.49203966896154 31.479424753679353 6 12.842086402614953 18.976742394684358 34.269239077496536 33.911932125204146 7 17.686599453606746 23.40602346458768 29.169030897148662 29.738346184656912 8 16.02445672878532 20.886149690838696 25.849862694806593 37.23953088556939 9 12.4403912624754 19.75842911232099 28.19547305608743 39.60570656911619 10-11 17.518385496285223 34.65756781709728 26.99183106565193 20.83221562096557 12-13 16.814582334468895 35.78050934550666 26.591849626857307 20.813058693167136 14-15 18.21511646912549 34.51260714897062 26.074935800393206 21.197340581510673 16-17 18.35198819520169 34.067193571511844 26.52948397805214 21.051334255234323 18-19 18.959087920027716 32.46768279277921 27.576450524674133 20.99677876251894 20-21 17.67759396175409 32.14486892160662 28.85632266794446 21.32121444869482 22-23 17.850978804294247 32.7727800027189 29.63498873612483 19.741252456862014 24-25 17.889578473136414 32.64059671908084 28.994979609959515 20.47484519782323 26-27 19.60755895454995 32.96425938130731 27.565884932524426 19.862296731618308 28-29 19.046968917403213 33.72604833209738 28.46801277669212 18.7589699738073 30-31 19.339621091925522 33.37940335431149 28.480751495839783 18.8002240579232 32-33 19.785788171043656 33.607460627093 27.981699553793526 18.625051648069817 34-35 19.796725951066023 33.721721042620764 27.794109917223775 18.68744308908944 36-37 20.314245268458443 33.39191745281986 27.53916229432967 18.754674984392025 38-39 20.700078226716794 33.592429271942706 27.503680082617304 18.20381241872319 40-41 20.94298297526493 33.99517572655172 27.391116077417045 17.670725220766307 42-43 21.11175555723672 34.132581795140915 27.5756274919751 17.180035155647268 44-45 21.322157411163285 33.80365625408111 27.552565476068747 17.32162085868686 46-47 21.393085106211668 33.33635750837606 27.848445995931055 17.422111389481216 48-49 21.297614031028534 33.394042053561435 28.168883760423242 17.13946015498679 50-51 21.507090988569647 33.313151933477194 28.437116818163506 16.742640259789656 52-53 21.569953777053268 33.27842180466272 28.68429787285298 16.46732654543103 54-55 21.542599634580075 33.33840377903335 28.64105721208366 16.47793937430292 56-57 21.487383134347933 33.42534459699176 28.181346687027688 16.905925581632626 58-59 21.247508853473562 33.13678664964464 28.39814930518429 17.21755519169751 60-61 21.156561481254414 32.912295168740286 28.814686062306727 17.116457287698577 62-63 21.187635404206322 32.409778863560994 28.989120515270823 17.413465216961857 64-65 21.048300133648038 32.2984106697389 28.949513296630307 17.70377589998276 66-67 20.68567612072787 32.03429797529365 29.42282657682499 17.8571993271535 68-69 20.410192993278358 32.2907133456844 29.209630381206452 18.089463279830788 70-71 19.997689191266215 32.63479886673766 29.25324457543607 18.114267366560053 72-73 19.9494286246093 32.9940064032172 29.302173628818213 17.754391343355294 74-75 19.78700890062791 33.51136049032432 29.071731578839405 17.629899030208367 76-77 19.32756815849258 34.21761248107981 29.01193287049076 17.442886489936857 78-79 18.82205505471889 35.05073754589989 28.74616569096713 17.381041708414088 80-81 18.396574523801657 35.957314796692614 28.465162747859402 17.180947931646333 82-83 18.02083898200621 36.60347752589223 28.443150833058223 16.932532659043336 84-85 17.705198649139483 37.462596310636094 28.179658137492048 16.652546902732375 86-87 17.471424752357727 38.199834266493994 27.818060495499005 16.510680485649278 88-89 17.382402828343434 38.66308722913112 27.6610246163023 16.29348532622315 90-91 17.375145963213264 39.11042858351382 27.47589531913312 16.038530134139794 92-93 17.50061787222998 39.44998664429929 27.06660670689639 15.982788776574342 94-95 17.75851590949871 39.33698474929931 26.746748613780518 16.15775072742147 96-97 18.067415307712672 38.95935869632389 26.772538781831006 16.200687214132422 98-99 18.327721848643883 38.70851057519062 26.659941709090546 16.30382586707494 100 18.876126158766045 38.28021003917704 26.42117009405388 16.42249370800303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 26297.0 1 18253.5 2 8505.5 3 6695.0 4 6966.0 5 7771.0 6 8880.5 7 10751.5 8 13717.5 9 19210.5 10 30096.0 11 48981.0 12 76556.5 13 112127.0 14 153112.5 15 193695.0 16 227688.0 17 253563.5 18 272474.0 19 286085.5 20 298783.0 21 314770.0 22 335837.0 23 360591.0 24 380818.5 25 390005.0 26 391674.5 27 389477.5 28 384003.0 29 371539.0 30 348995.5 31 318794.5 32 283091.5 33 246537.5 34 213790.0 35 185717.5 36 163098.0 37 146629.5 38 135269.5 39 128652.5 40 128109.5 41 133987.5 42 148721.5 43 174378.0 44 211793.5 45 259124.5 46 308968.0 47 355610.0 48 396384.5 49 435257.5 50 482641.5 51 545014.5 52 616166.0 53 675577.5 54 696810.5 55 657774.0 56 559664.0 57 427338.0 58 292068.0 59 180341.0 60 102320.0 61 55154.5 62 30270.5 63 18138.0 64 12403.0 65 9543.5 66 7713.5 67 6350.0 68 5527.0 69 4828.5 70 3976.5 71 3302.0 72 2885.5 73 2552.0 74 2255.5 75 2027.0 76 1826.5 77 1680.0 78 1603.5 79 1560.5 80 1496.5 81 1415.5 82 1402.5 83 1372.5 84 1357.0 85 1400.5 86 1370.5 87 1293.0 88 1254.5 89 1240.5 90 1272.5 91 1280.0 92 1235.0 93 1209.5 94 1185.5 95 1170.0 96 1146.0 97 1104.5 98 1042.5 99 970.5 100 990.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14160422429872394 2 0.1337044429322496 3 0.13519047672350007 4 0.1323149048417297 5 0.1302949281731035 6 0.11597496618468994 7 0.11700425365914913 8 0.11819436730149259 9 0.11757679481681706 10-11 0.11726800857447932 12-13 0.12074828684749447 14-15 0.12809160967308927 16-17 0.12122433230443184 18-19 0.12837144720520788 20-21 0.12925920765192894 22-23 0.1293267546424403 24-25 0.13022416465923442 26-27 0.13083852062055226 28-29 0.13105081116215947 30-31 0.12979958357602 32-33 0.129912161893539 34-35 0.13168446626362343 36-37 0.1326365571774982 38-39 0.13268802155122114 40-41 0.12941038424974013 42-43 0.12721028227308362 44-45 0.12667633939570788 46-47 0.12762521378622496 48-49 0.12902118492346024 50-51 0.12681143337673068 52-53 0.1271234361424261 54-55 0.12949079733368227 56-57 0.12635790358329707 58-59 0.12707840481541852 60-61 0.12849045856944225 62-63 0.1236978387664916 64-65 0.11663756999637305 66-67 0.11683377792119183 68-69 0.11497141089709222 70-71 0.11388100947883702 72-73 0.11684986053798026 74-75 0.11809465507740435 76-77 0.11579805740001725 78-79 0.11146861696057328 80-81 0.1121473033890448 82-83 0.11250112095839014 84-85 0.1084225693408456 86-87 0.11191571370729149 88-89 0.11155546309123075 90-91 0.11665365261316148 92-93 0.11358508932992999 94-95 0.11561471556862922 96-97 0.11851923616061878 98-99 0.1221346084146567 100 0.1226074373482364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.5544734E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.20423796893141 #Duplication Level Percentage of deduplicated Percentage of total 1 92.28974737698906 83.24926334486484 2 6.531159051043868 11.782764505066027 3 0.8006159643394076 2.166568589069923 4 0.1671798252847259 0.6032131497435118 5 0.0682061786721026 0.30762431859448985 6 0.039821901160509605 0.21552625491947247 7 0.023471815610427778 0.14820800686201346 8 0.016793667634161194 0.1211887993314414 9 0.01082280191674386 0.08786363396297131 >10 0.048278735685205784 0.7568665415728049 >50 0.002642274500940866 0.16022998541171485 >100 0.0011664448359302805 0.18091402204528617 >500 5.1147813152790535E-5 0.027297683345813693 >1k 2.8545531074312634E-5 0.03907188043518519 >5k 7.13449135335391E-6 0.05328688026232819 >10k+ 7.13449135335391E-6 0.10011240451217117 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 15556 0.10007247470429537 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003261554684692578 0.0 0.0 0.0 0.0 2 0.007629593404428792 0.0 0.0 1.929914014610993E-5 0.0 3 0.013509398102276951 0.0 0.0 2.5732186861479906E-5 0.0 4 0.02242560084977974 0.0 0.0 3.2165233576849886E-5 0.0 5 0.03625021824110982 0.0 0.0 3.859828029221986E-5 0.0 6 0.06080515755367702 0.0 0.0 4.503132700758984E-5 0.0 7 0.09083461962102407 0.0 0.0 4.503132700758984E-5 0.0 8 0.19265688303190007 0.0 0.0 4.503132700758984E-5 0.0 9 0.5603505341423018 0.0 0.0 4.503132700758984E-5 0.0 10-11 1.3771705582096163 0.0 0.0 4.503132700758984E-5 0.0 12-13 2.3944700501147205 0.0 0.0 4.503132700758984E-5 0.0 14-15 3.500947652111641 0.0 0.0 6.111394379601478E-5 0.0 16-17 4.640394618524834 0.0 0.0 6.433046715369977E-5 0.0 18-19 5.840260759688779 0.0 0.0 7.719656058443973E-5 0.0 20-21 7.327317405366988 0.0 0.0 9.649570073054965E-5 0.0 22-23 8.982353766876937 0.0 0.0 9.971222408823463E-5 0.0 24-25 10.69551913850697 0.0 0.0 1.0292874744591963E-4 0.0 26-27 12.418806909143637 0.0 0.0 1.0292874744591963E-4 0.0 28-29 14.227129907787422 0.0 0.0 1.0292874744591963E-4 0.0 30-31 16.180678936030684 0.0 0.0 1.093617941612896E-4 0.0 32-33 18.331487692230695 0.0 0.0 1.093617941612896E-4 0.0 34-35 20.31762975165738 0.0 0.0 1.1579484087665959E-4 0.0 36-37 21.91786298819909 0.0 0.0 1.383105043804545E-4 6.433046715369977E-6 38-39 23.178495688636424 0.0 0.0 2.0585749489183925E-4 6.433046715369977E-6 40-41 24.332835158195692 0.0 0.0 2.3802272846868913E-4 6.433046715369977E-6 42-43 25.314559901764802 0.0 0.0 2.76621008760909E-4 6.433046715369977E-6 44-45 26.147353180826382 0.0 0.0 2.83054055476279E-4 6.433046715369977E-6 46-47 26.877754871842775 0.0 0.0 2.9270362554933396E-4 6.433046715369977E-6 48-49 27.554244414861007 0.0 0.0 3.4738452262997874E-4 6.433046715369977E-6 50-51 28.220875313787936 0.0 0.0 4.213645598567335E-4 6.433046715369977E-6 52-53 28.90603338725513 0.0 0.0 4.889115503681182E-4 6.433046715369977E-6 54-55 29.604501434376424 3.2165233576849883E-6 0.0 5.789742043832979E-4 6.433046715369977E-6 56-57 30.318904138211693 6.433046715369977E-6 0.0 6.240055313908877E-4 6.433046715369977E-6 58-59 31.045918830132443 6.433046715369977E-6 0.0 6.658203350407925E-4 6.433046715369977E-6 60-61 31.774612547245905 6.433046715369977E-6 0.0 7.108516620483825E-4 6.433046715369977E-6 62-63 32.479298134017604 6.433046715369977E-6 0.0 7.655325591290272E-4 6.433046715369977E-6 64-65 33.14884641962995 6.433046715369977E-6 0.0 8.813274000056869E-4 6.433046715369977E-6 66-67 33.77684365650772 6.433046715369977E-6 6.433046715369977E-6 9.681735306631815E-4 6.433046715369977E-6 68-69 34.35452481850123 6.433046715369977E-6 6.433046715369977E-6 0.0010099883343130864 6.433046715369977E-6 70-71 34.8997673424325 6.433046715369977E-6 6.433046715369977E-6 0.0010325039978168814 6.433046715369977E-6 72-73 35.423102125774555 6.433046715369977E-6 6.433046715369977E-6 0.001055019661320676 1.2866093430739953E-5 74-75 35.92732754384861 6.433046715369977E-6 6.433046715369977E-6 0.001103267511685951 1.929914014610993E-5 76-77 36.415727667002855 6.433046715369977E-6 6.433046715369977E-6 0.0011933301657011306 1.929914014610993E-5 78-79 36.89252257388257 6.433046715369977E-6 6.433046715369977E-6 0.0013670224270161202 1.929914014610993E-5 80-81 37.354682299484836 6.433046715369977E-6 6.433046715369977E-6 0.001640426912419344 1.929914014610993E-5 82-83 37.81044114360529 6.433046715369977E-6 6.433046715369977E-6 0.0017369226131498938 1.929914014610993E-5 84-85 38.26138485225929 6.433046715369977E-6 6.433046715369977E-6 0.0017690878467267435 1.929914014610993E-5 86-87 38.70786402649283 6.433046715369977E-6 6.433046715369977E-6 0.0017787374167997985 1.929914014610993E-5 88 39.039014755736574 6.433046715369977E-6 6.433046715369977E-6 0.0017883869868728535 1.929914014610993E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTGA 8475 0.0 47.636833 9 GCTTGCT 1780 0.0 45.149788 7 CCTGCTG 4820 0.0 41.440434 8 GCCTGCT 1700 0.0 39.810093 7 CTTGCTG 5480 0.0 39.36539 8 CTGCTGA 7285 0.0 37.99926 9 GTTGCTG 2265 0.0 36.312176 8 TACCCGA 9635 0.0 36.19302 6 ACCCGAC 10640 0.0 33.52585 7 ACTTGCT 1510 0.0 32.991993 7 CTACCCG 9770 0.0 32.473625 5 CATGCTG 940 0.0 31.998863 8 CCCGACG 12360 0.0 30.115433 8 ACTCGCC 1415 0.0 29.896685 3 TCGCGAC 865 0.0 29.882656 6 ATTGCTG 1475 0.0 28.99558 8 GCTGAAC 16730 0.0 28.809341 10-11 TGAACCG 15285 0.0 28.797132 12-13 GACACGC 1090 0.0 28.030499 2 ACTTGCC 1030 0.0 27.837458 3 >>END_MODULE