FastQCFastQC Report
Thu 26 May 2016
SRR1248455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15544734
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA100150.049.566296
GCCCTAC281900.042.1526347
GGCATAT13750.041.3695955
CCCCTAC664950.041.2684177
GCCCCTA77300.040.37966
CCCCCTA360200.039.3344426
CGCCCTA107600.038.882236
ATGCCCT33850.038.747545
GTCCCTA41700.038.4407426
CCGCCTA51100.038.360886
TAGGCAT16000.037.894613
TTGCCCT47350.037.6285825
GCATATC15450.037.423996
CTTAGGC16300.037.2003971
TGCTCTA103950.037.081976
CCCGCTA36100.036.8512886
AGGCATA16300.036.6204644
GCTGCGT15000.036.35515
TTGCTCT38900.036.2551965
TTAGGCA17200.035.251032
Produced by FastQC (version 0.11.5)