##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248454_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14038511
Sequences flagged as poor quality	0
Sequence length	100
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.291831092343056	33.0	30.0	34.0	16.0	34.0
2	29.84971226649322	33.0	30.0	34.0	18.0	34.0
3	30.424010138967017	33.0	31.0	34.0	25.0	34.0
4	33.9705574900358	37.0	35.0	37.0	28.0	37.0
5	33.818192969325594	37.0	35.0	37.0	28.0	37.0
6	33.5476449745988	37.0	35.0	37.0	26.0	37.0
7	33.89859914630547	37.0	35.0	37.0	28.0	37.0
8	33.86558132839017	37.0	35.0	37.0	28.0	37.0
9	35.30937397847963	39.0	35.0	39.0	27.0	39.0
10-11	36.22477123820325	39.0	37.0	39.0	31.0	39.0
12-13	36.32930251648483	39.0	37.0	39.0	31.0	39.0
14-15	37.69430824964272	40.0	38.0	41.0	31.0	41.0
16-17	37.51619178130786	40.0	38.0	41.0	31.0	41.0
18-19	36.748967394049124	39.0	36.0	41.0	30.0	41.0
20-21	36.21805068927894	39.0	35.0	41.0	30.0	41.0
22-23	35.831916718233146	38.0	35.0	41.0	29.0	41.0
24-25	35.54700544808492	38.0	35.0	41.0	27.0	41.0
26-27	35.37830297671883	38.0	35.0	41.0	25.0	41.0
28-29	35.224184316983475	37.0	35.0	40.0	25.0	41.0
30-31	35.14530821680448	37.0	34.5	40.0	25.0	41.0
32-33	35.08289048603517	37.0	34.0	40.0	25.0	41.0
34-35	35.00434209155088	37.0	34.0	40.0	25.0	41.0
36-37	34.90915870636138	37.0	34.0	40.0	25.0	41.0
38-39	34.8089153472188	36.5	33.5	40.0	25.0	41.0
40-41	34.68858666706177	36.0	33.0	40.0	25.0	41.0
42-43	34.55354232368376	35.5	33.0	40.0	25.0	41.0
44-45	34.40035264423699	35.0	33.0	40.0	25.0	41.0
46-47	34.24302926428594	35.0	33.0	40.0	25.0	41.0
48-49	34.05752917813007	35.0	33.0	39.5	25.0	41.0
50-51	33.837411068737985	35.0	33.0	39.0	25.0	41.0
52-53	33.585784097757944	35.0	32.5	39.0	25.0	41.0
54-55	33.42103186726854	35.0	32.0	38.5	25.0	41.0
56-57	33.25089940094074	35.0	31.5	38.0	25.0	40.0
58-59	33.28901127762054	35.0	32.0	37.5	25.0	40.0
60-61	33.13780499940485	35.0	32.0	37.0	25.0	40.0
62-63	32.947453722121956	35.0	31.0	36.5	25.0	40.0
64-65	32.77166285655224	35.0	31.0	36.0	25.0	40.0
66-67	32.60900564881845	35.0	31.0	35.0	25.0	39.0
68-69	32.4469089350003	35.0	31.0	35.0	25.0	39.0
70-71	32.2916898024299	35.0	31.0	35.0	25.0	39.0
72-73	32.266104111753734	35.0	31.0	35.0	25.0	38.5
74-75	32.30014130415968	35.0	31.0	35.0	25.0	37.5
76-77	32.190956790217996	35.0	31.0	35.0	25.0	37.0
78-79	32.025245839818766	35.0	31.0	35.0	25.0	36.5
80-81	31.852961008471624	35.0	31.0	35.0	25.0	36.0
82-83	31.67438006780064	35.0	30.5	35.0	24.5	36.0
84-85	31.499593083625463	35.0	30.0	35.0	24.0	36.0
86-87	31.320212806044744	35.0	30.0	35.0	24.0	35.5
88-89	31.13438041968981	35.0	30.0	35.0	23.5	35.0
90-91	30.93066821687856	35.0	30.0	35.0	22.0	35.0
92-93	30.74020645779314	35.0	29.5	35.0	20.0	35.0
94-95	30.53371525655392	35.0	29.0	35.0	20.0	35.0
96-97	30.31536759845827	34.0	29.0	35.0	18.5	35.0
98-99	30.105498474873865	34.0	28.5	35.0	17.0	35.0
100	29.402048265660085	34.0	27.0	35.0	13.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4379963154627795
1101	2	0.3708240452197238
1101	3	0.3149895932504627
1101	4	0.2682961908691581
1101	5	0.1571216726065785
1101	6	0.16654886321357054
1101	7	0.1635765508498963
1101	8	0.22777886426316485
1101	9	0.2678952543349453
1101	10-11	0.3150219929277469
1101	12-13	0.3206383351111626
1101	14-15	0.4761160427566935
1101	16-17	0.4720652598453583
1101	18-19	0.49341736580701934
1101	20-21	0.5007238418360558
1101	22-23	0.48496748619741936
1101	24-25	0.47346914531869544
1101	26-27	0.48396413940219674
1101	28-29	0.49226089560825415
1101	30-31	0.5071261434015852
1101	32-33	0.5045829161191691
1101	34-35	0.514858665199462
1101	36-37	0.5262408480761138
1101	38-39	0.5533983576850545
1101	40-41	0.5443957451438877
1101	42-43	0.5862480866718371
1101	44-45	0.5710278927621459
1101	46-47	0.5718604744928584
1101	48-49	0.5326974031733513
1101	50-51	0.549456263398973
1101	52-53	0.5170312664673062
1101	54-55	0.4700257541833892
1101	56-57	0.4860374757610657
1101	58-59	0.46027755467243026
1101	60-61	0.42302524841147715
1101	62-63	0.43810480992637935
1101	64-65	0.42720375722194603
1101	66-67	0.42034109883805826
1101	68-69	0.4185392350430348
1101	70-71	0.42440723748451603
1101	72-73	0.4000747403750964
1101	74-75	0.42753786644460234
1101	76-77	0.42332949893472005
1101	78-79	0.384716928357804
1101	80-81	0.3826411886683232
1101	82-83	0.3573692552882939
1101	84-85	0.3919379943400436
1101	86-87	0.4024628522615288
1101	88-89	0.42150822720127223
1101	90-91	0.3925758166595479
1101	92-93	0.35420143445947616
1101	94-95	0.3423616881725522
1101	96-97	0.3832106652115499
1101	98-99	0.4526288078461107
1101	100	0.4680974942924614
1102	1	0.2911190652689193
1102	2	0.2967064097003558
1102	3	0.24199341973397281
1102	4	0.20213697176720302
1102	5	0.17347844719023442
1102	6	0.10454263652327
1102	7	0.12944497566803648
1102	8	0.15078784657548283
1102	9	0.1643175614875645
1102	10-11	0.24025128191264145
1102	12-13	0.2732935927179767
1102	14-15	0.3975109725506485
1102	16-17	0.403914157596553
1102	18-19	0.3909603560492556
1102	20-21	0.3936529249570597
1102	22-23	0.3418552430656945
1102	24-25	0.2959248972757891
1102	26-27	0.3056174953067128
1102	28-29	0.32022748773003684
1102	30-31	0.3571204602196474
1102	32-33	0.36277740095170685
1102	34-35	0.34962563166118343
1102	36-37	0.3653968465159778
1102	38-39	0.3450453784283809
1102	40-41	0.3185164386190138
1102	42-43	0.3744134805365391
1102	44-45	0.351707439769271
1102	46-47	0.33259262987855465
1102	48-49	0.33359598058925855
1102	50-51	0.3272804009400616
1102	52-53	0.31934210016989795
1102	54-55	0.27935727928400667
1102	56-57	0.29956229482240104
1102	58-59	0.27768566021691043
1102	60-61	0.2807922829559928
1102	62-63	0.2685525869656331
1102	64-65	0.2604997892647063
1102	66-67	0.3065558919586948
1102	68-69	0.31758359501866806
1102	70-71	0.3035463248520429
1102	72-73	0.3237645794227717
1102	74-75	0.3028441812963436
1102	76-77	0.3192328190669116
1102	78-79	0.2861556099980689
1102	80-81	0.2839597157260343
1102	82-83	0.2907228115177496
1102	84-85	0.258361351583126
1102	86-87	0.2836822438552673
1102	88-89	0.27646895266377314
1102	90-91	0.28227408297172474
1102	92-93	0.23420745228562723
1102	94-95	0.24865524961784402
1102	96-97	0.2885335276427732
1102	98-99	0.240827521889166
1102	100	0.19635444796847068
1103	1	-0.02206002364979298
1103	2	-0.047211282874670246
1103	3	0.07908819814812773
1103	4	0.06440904014819893
1103	5	0.012842010288828476
1103	6	-0.026403108339174253
1103	7	0.0033243102034603567
1103	8	0.035471267357998215
1103	9	0.05658946350353489
1103	10-11	0.12149931778721879
1103	12-13	0.1114491935217643
1103	14-15	0.21229015247825345
1103	16-17	0.2344288833489756
1103	18-19	0.17705715791697685
1103	20-21	0.1979415206309696
1103	22-23	0.17387793027199905
1103	24-25	0.16029289760759724
1103	26-27	0.15462121385007066
1103	28-29	0.18457654942218937
1103	30-31	0.14317549306522181
1103	32-33	0.18274992286750802
1103	34-35	0.22286765768586037
1103	36-37	0.2186783793275282
1103	38-39	0.1712609756790897
1103	40-41	0.23769988757075566
1103	42-43	0.220394989736711
1103	44-45	0.1732674739465665
1103	46-47	0.20257529559479792
1103	48-49	0.23773055668520016
1103	50-51	0.2166723148487435
1103	52-53	0.18197244688901293
1103	54-55	0.20448200325014199
1103	56-57	0.2127471121984641
1103	58-59	0.20804461518056883
1103	60-61	0.19472249909765793
1103	62-63	0.19439498855920334
1103	64-65	0.21060454663629713
1103	66-67	0.20388012970290958
1103	68-69	0.21597268058674501
1103	70-71	0.22776827467284022
1103	72-73	0.22596918311268865
1103	74-75	0.24266818237734356
1103	76-77	0.2148686457183615
1103	78-79	0.19509136263970817
1103	80-81	0.17944593285371724
1103	82-83	0.21740046280110903
1103	84-85	0.20510233857858395
1103	86-87	0.169298182922347
1103	88-89	0.18891342951149426
1103	90-91	0.19080760070873382
1103	92-93	0.20432690519538355
1103	94-95	0.17361049398794393
1103	96-97	0.1792729350930955
1103	98-99	0.22190754612897834
1103	100	0.1958625781027301
1104	1	0.05336177425943589
1104	2	0.03931953231853669
1104	3	0.07606613478423796
1104	4	0.09765685122889778
1104	5	0.06936662095873203
1104	6	-0.033389270711666086
1104	7	-0.019423881943254173
1104	8	0.04156413421115701
1104	9	0.048806023521507313
1104	10-11	0.16268535887321178
1104	12-13	0.19612291528385128
1104	14-15	0.23409939279117253
1104	16-17	0.26064814063149555
1104	18-19	0.21333278707098913
1104	20-21	0.19972644218659497
1104	22-23	0.21483095432516564
1104	24-25	0.20351839125294902
1104	26-27	0.17405557817902917
1104	28-29	0.16827470458305527
1104	30-31	0.1911704651913695
1104	32-33	0.20384593313637822
1104	34-35	0.19485762811214613
1104	36-37	0.21892127882202317
1104	38-39	0.21154306234413411
1104	40-41	0.252814200449464
1104	42-43	0.23739424774272067
1104	44-45	0.22813607129560864
1104	46-47	0.24629119885570105
1104	48-49	0.2017626597604263
1104	50-51	0.21630962823103062
1104	52-53	0.2261325382685868
1104	54-55	0.22451501786036943
1104	56-57	0.24266396988513605
1104	58-59	0.23081114283293402
1104	60-61	0.23365090388657705
1104	62-63	0.18565010056224907
1104	64-65	0.2241573468909408
1104	66-67	0.2033507801155352
1104	68-69	0.17784729577055458
1104	70-71	0.2237972864745501
1104	72-73	0.22582833471323482
1104	74-75	0.2074935724900584
1104	76-77	0.2136438665898055
1104	78-79	0.24101653794605937
1104	80-81	0.2422061642092288
1104	82-83	0.2274317992859345
1104	84-85	0.189099023920825
1104	86-87	0.1971490368217701
1104	88-89	0.19334019481182452
1104	90-91	0.20794679760946977
1104	92-93	0.18144219405634843
1104	94-95	0.19865052282760942
1104	96-97	0.2901901730844365
1104	98-99	0.29322166262874205
1104	100	0.3067498285021557
1105	1	0.11563219033037342
1105	2	0.0939187365932348
1105	3	0.06655241265541534
1105	4	0.14139349555593128
1105	5	0.04809491111728903
1105	6	0.059398896199390094
1105	7	0.053997393010419614
1105	8	0.07146451868326409
1105	9	0.1554393902922797
1105	10-11	0.14834284043104162
1105	12-13	0.10468694680301383
1105	14-15	0.1864948599362677
1105	16-17	0.2113444035148646
1105	18-19	0.17018450275392638
1105	20-21	0.1575224053267874
1105	22-23	0.12027975099921662
1105	24-25	0.12769697803412328
1105	26-27	0.1267528321183775
1105	28-29	0.17543549919767543
1105	30-31	0.15310548835429216
1105	32-33	0.18892906804442333
1105	34-35	0.2204096474568047
1105	36-37	0.2188716676343816
1105	38-39	0.1783544104670156
1105	40-41	0.17265646321268946
1105	42-43	0.1914568700757897
1105	44-45	0.2252553915542137
1105	46-47	0.2557264591428421
1105	48-49	0.2080273194544091
1105	50-51	0.21748227399625364
1105	52-53	0.22864695029588944
1105	54-55	0.20660358852487803
1105	56-57	0.18761005153553612
1105	58-59	0.19291178315266677
1105	60-61	0.18092985318559585
1105	62-63	0.16444642688202293
1105	64-65	0.18773554033784734
1105	66-67	0.1927191616412074
1105	68-69	0.20081513471968293
1105	70-71	0.23307687519799458
1105	72-73	0.2154025050282442
1105	74-75	0.172832479767159
1105	76-77	0.18145257669762316
1105	78-79	0.16856679757599835
1105	80-81	0.19521928317966797
1105	82-83	0.18154064624460275
1105	84-85	0.17453718990414657
1105	86-87	0.1773325495585496
1105	88-89	0.19330521980036863
1105	90-91	0.1983498452039001
1105	92-93	0.14085490970152392
1105	94-95	0.16461959663921988
1105	96-97	0.1895886885246263
1105	98-99	0.2421114995819984
1105	100	0.28692296097213443
1106	1	0.05502153626889594
1106	2	0.04819135861570345
1106	3	0.09755165702707203
1106	4	0.12846503046718993
1106	5	0.04243411803602015
1106	6	0.0918405940006437
1106	7	0.015996729472973925
1106	8	0.08787663423735381
1106	9	0.0552207649789338
1106	10-11	0.07936018508704734
1106	12-13	0.07666681701966382
1106	14-15	0.14125836191669094
1106	16-17	0.14959337732485523
1106	18-19	0.16531465020556624
1106	20-21	0.15542843776836435
1106	22-23	0.12941654782257928
1106	24-25	0.12351399123190276
1106	26-27	0.110318175611134
1106	28-29	0.1287689776782841
1106	30-31	0.13532474117701554
1106	32-33	0.14473038537548177
1106	34-35	0.14489849062142923
1106	36-37	0.10864867755267937
1106	38-39	0.10415354171743729
1106	40-41	0.07439406709926288
1106	42-43	0.10550610871424482
1106	44-45	0.12296675907246879
1106	46-47	0.14716095362235393
1106	48-49	0.15408119994714298
1106	50-51	0.1321159189428016
1106	52-53	0.13992098039480538
1106	54-55	0.15874199973588787
1106	56-57	0.11483539894209116
1106	58-59	0.11182031974209394
1106	60-61	0.0961159695750311
1106	62-63	0.1297604601618545
1106	64-65	0.1165971978068896
1106	66-67	0.04382878669720469
1106	68-69	0.10570769332930041
1106	70-71	0.14785833749051847
1106	72-73	0.1044469376654007
1106	74-75	0.10442304896947263
1106	76-77	0.13347727770840834
1106	78-79	0.20751540713531114
1106	80-81	0.17160982304695693
1106	82-83	0.17481889433816633
1106	84-85	0.16358749003988393
1106	86-87	0.11657436770636309
1106	88-89	0.10439174992854561
1106	90-91	0.10316642697666012
1106	92-93	0.11887402049677931
1106	94-95	0.1332924623513989
1106	96-97	0.1587507005596258
1106	98-99	0.14257191462977659
1106	100	0.15452600993238264
1107	1	-0.08565532507148177
1107	2	0.06488638390858625
1107	3	0.03313427320916418
1107	4	0.05246814047555404
1107	5	0.06858141741236068
1107	6	0.06988760046998976
1107	7	0.03472786876188394
1107	8	0.14601750795912238
1107	9	0.07606243719725114
1107	10-11	0.12296777625462596
1107	12-13	0.16179253645972835
1107	14-15	0.16411116712758655
1107	16-17	0.15039956104551067
1107	18-19	0.13472461109673617
1107	20-21	0.14489889668184475
1107	22-23	0.13976235892292266
1107	24-25	0.10364592216086521
1107	26-27	0.12955762323883135
1107	28-29	0.07978640293178785
1107	30-31	0.10580006503663242
1107	32-33	0.10029597183604011
1107	34-35	0.09242865406899625
1107	36-37	0.11222119634685868
1107	38-39	0.08116917892004949
1107	40-41	0.08277650395147873
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2216	40-41	-0.10170121866826776
2216	42-43	-0.17662028363066895
2216	44-45	-0.15636436894374128
2216	46-47	-0.21901068937914658
2216	48-49	-0.15791994023188494
2216	50-51	-0.1596289986566788
2216	52-53	-0.15482899676293727
2216	54-55	-0.21162807137328343
2216	56-57	-0.21663904790128896
2216	58-59	-0.18404974060936752
2216	60-61	-0.2221611570435016
2216	62-63	-0.26057603710181354
2216	64-65	-0.26024412029168076
2216	66-67	-0.24455908048287256
2216	68-69	-0.29086725052776075
2216	70-71	-0.2950664629825326
2216	72-73	-0.2716010630888164
2216	74-75	-0.2611617066132368
2216	76-77	-0.28230567868088485
2216	78-79	-0.2914021851999351
2216	80-81	-0.32056073074427616
2216	82-83	-0.34538825535263484
2216	84-85	-0.34758329229850204
2216	86-87	-0.34353091609689557
2216	88-89	-0.35117709393197316
2216	90-91	-0.40016742813570616
2216	92-93	-0.45308478884685144
2216	94-95	-0.39606035507677007
2216	96-97	-0.3351950381684894
2216	98-99	-0.34640191511044804
2216	100	-0.30521776121158695
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	15769.0
1	846.0
2	407.0
3	381.0
4	307.0
5	818.0
6	3757.0
7	8289.0
8	13966.0
9	21320.0
10	30373.0
11	39215.0
12	42898.0
13	45528.0
14	50352.0
15	57667.0
16	63842.0
17	68475.0
18	73082.0
19	77972.0
20	85392.0
21	90645.0
22	96166.0
23	111793.0
24	234617.0
25	326350.0
26	158902.0
27	462043.0
28	182313.0
29	412170.0
30	251151.0
31	300739.0
32	398080.0
33	887972.0
34	861925.0
35	3425059.0
36	1682634.0
37	1641681.0
38	1345427.0
39	464005.0
40	4183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.919990988115469	35.46452654860375	24.531609584501613	25.083872878779168
2	11.536319578132531	41.12337536205015	24.70946284930175	22.63084221051557
3	9.822520900308698	44.893921579453696	22.00037750886097	23.28318001137663
4	8.781601894788668	44.795198343122784	21.850924278716775	24.572275483371772
5	8.710765901643834	44.27680204934357	22.519311034338717	24.493121014673875
6	8.49165193939594	41.875977682398904	23.306172487477927	26.326197890727226
7	11.375897475069829	46.91378840232588	19.632921311619693	22.077392810984602
8	10.137408417449533	43.9610934816019	17.285675934212623	28.615822166735942
9	8.157447973654444	42.50300608391872	18.763826399090547	30.575719543336287
10-11	10.82006626437881	56.46129758874062	18.088717581571274	14.6299185653093
12-13	10.431726514483167	57.19576762598205	17.92402108626549	14.448484773269296
14-15	11.272298628285911	56.35377518109388	17.610545159055608	14.763381031564604
16-17	11.353398345910342	56.15990874283238	17.936433531174647	14.550259380082636
18-19	11.653992220500054	55.12788825401934	18.635248506097337	14.582871019383267
20-21	10.950799563621485	54.872572423383026	19.345532805355468	14.831095207640018
22-23	11.07108014141087	55.28447913607576	19.796944248619035	13.847496473894335
24-25	11.062473531232628	55.22628731922327	19.480838021237215	14.230401128306887
26-27	12.080609322832798	55.38797593270539	18.686286116786732	13.845128627675077
28-29	11.724861961609259	55.891390420469165	19.244875343691607	13.138872274229968
30-31	11.905977521633137	55.642872711075164	19.241333094561213	13.209816672730485
32-33	12.199205207577023	55.7453820938127	18.917013380633353	13.138399317976923
34-35	12.241308204260806	55.81199712254469	18.835522983993485	13.111171689201015
36-37	12.5325912300313	55.677771144891864	18.675637505164147	13.114000119912687
38-39	12.763366495793793	55.74889316886461	18.66528411379482	12.822456221546775
40-41	12.934039675709657	55.972386865079095	18.587489297099744	12.506084162111502
42-43	13.042233707461554	56.07010797293198	18.679343624207316	12.208314695399151
44-45	13.174250562051935	55.87750450902303	18.632708649688347	12.315536279236696
46-47	13.230547766581651	55.59785205855919	18.82382933478733	12.34777084007182
48-49	13.180270901786423	55.60399725259146	19.03399657708105	12.181735268541066
50-51	13.320636639342027	55.523095767845945	19.183442084440504	11.972825508371526
52-53	13.39783801581586	55.503103426237345	19.301759055202584	11.79729950274421
54-55	13.365279068240444	55.55432111812117	19.298588132097898	11.78181168154049
56-57	13.348415716697518	55.55110182131655	19.07674457761225	12.02373788437368
58-59	13.236147874790461	55.34268464857885	19.257398365273364	12.163769111357327
60-61	13.199094421796193	55.196002285309206	19.46516289069573	12.13974040219887
62-63	13.25658225096121	54.88747576289117	19.554007953309544	12.301934032838073
64-65	13.166791218663429	54.825586313249964	19.55711459121293	12.450507876873683
66-67	12.966597213336694	54.6758288289946	19.822574486164466	12.534999471504236
68-69	12.863901574981313	54.75824184327812	19.657826937953487	12.720029643787079
70-71	12.673932211467106	54.89749944709275	19.72386517436031	12.704703167079835
72-73	12.630064704805811	55.095943871244835	19.756947232939158	12.517044191010196
74-75	12.511941693190787	55.421549213969215	19.627131384283537	12.439377708556462
76-77	12.221680418473145	55.832095374767135	19.62191964420397	12.32430456255576
78-79	11.935381874687739	56.24581340900841	19.535487154357632	12.283317561946214
80-81	11.68234603586892	56.78919182604565	19.334616352900984	12.19384578518444
82-83	11.463127601448003	57.20079666124596	19.324569565149794	12.011506172156253
84-85	11.23477341826564	57.728582586939694	19.215754933400895	11.820889061393771
86-87	11.083051325955005	58.191346046080106	19.021522326707878	11.704080301257017
88-89	11.019894310417284	58.48851309102715	18.891145506552423	11.60044709200314
90-91	11.026826084379632	58.75360879792465	18.753720773274665	11.465844344421058
92-93	11.106117158671028	58.94315429978177	18.518188995844817	11.432539545702397
94-95	11.246341310160972	58.9187587738483	18.332939759024114	11.50196015696661
96-97	11.42679313077992	58.71807053477123	18.326140242903477	11.52899609154538
98-99	11.608796120870968	58.51976341920553	18.264637897256147	11.60680256266736
100	11.939801458965196	58.31096897206106	18.101216869468733	11.64801269950501
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2145489.0
1	1840929.0
2	958486.0
3	250344.0
4	117914.0
5	106132.5
6	81326.0
7	54095.5
8	36086.0
9	29422.5
10	30641.0
11	36641.5
12	46589.0
13	59823.5
14	75923.0
15	93679.5
16	112023.5
17	130293.5
18	148629.5
19	167605.0
20	186144.0
21	204185.0
22	223187.0
23	242012.0
24	256760.0
25	265186.5
26	267727.0
27	267764.0
28	265855.5
29	259571.0
30	247446.0
31	229473.0
32	209031.5
33	188889.5
34	169855.0
35	153114.0
36	138918.5
37	126904.5
38	117270.5
39	110147.0
40	105407.5
41	104169.0
42	108538.5
43	117979.0
44	133459.5
45	154313.5
46	176747.0
47	198614.0
48	218309.5
49	238190.0
50	262832.0
51	294781.5
52	329940.0
53	357515.0
54	364672.0
55	341257.5
56	289263.5
57	221025.5
58	151925.5
59	94516.0
60	54487.0
61	30558.0
62	17552.5
63	11121.5
64	8026.5
65	6342.5
66	5387.5
67	4618.0
68	3950.5
69	3541.5
70	3222.5
71	2917.0
72	2659.5
73	2376.5
74	2186.0
75	2123.5
76	2009.5
77	1869.0
78	1759.0
79	1709.5
80	1666.5
81	1631.0
82	1599.5
83	1563.5
84	1545.0
85	1512.0
86	1492.5
87	1432.0
88	1412.5
89	1418.5
90	1369.5
91	1330.5
92	1296.0
93	1244.5
94	1216.0
95	1162.0
96	1088.0
97	1073.5
98	981.0
99	806.5
100	696.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1529008311494004
2	0.1430351124845078
3	0.14458086046305055
4	0.14139676209250396
5	0.13949485098526476
6	0.12444339716655135
7	0.12571133790471084
8	0.12698640190544425
9	0.1266373620393217
10-11	0.12611736387142483
12-13	0.12964337884552002
14-15	0.13721540696160725
16-17	0.13072611475675733
18-19	0.13789211690613057
20-21	0.13882526430331535
22-23	0.1389321132419243
24-25	0.13982964432623946
26-27	0.14058114852778902
28-29	0.14079840803629387
30-31	0.13937019389022096
32-33	0.1398332059575264
34-35	0.14157128202556524
36-37	0.14230497807068
38-39	0.14246168984730645
40-41	0.13912087970013343
42-43	0.13651020396678823
44-45	0.13620746530739622
46-47	0.13696253114023274
48-49	0.13877540146529785
50-51	0.13615047920680476
52-53	0.13653157375451
54-55	0.1391137564375595
56-57	0.13580143934068223
58-59	0.13651020396678823
60-61	0.1382019788280965
62-63	0.13311240771902377
64-65	0.12531955846314471
66-67	0.12526257236255325
68-69	0.12366339991470604
70-71	0.12232066491952032
72-73	0.12540503761403185
74-75	0.12666229345833044
76-77	0.12425819233962919
78-79	0.11974560549904473
80-81	0.12039026076198538
82-83	0.1210456009187869
84-85	0.11656150712849817
86-87	0.12011601515288907
88-89	0.11958889372241828
90-91	0.12514147689879646
92-93	0.12186121448350186
94-95	0.12422969928933346
96-97	0.1273639348218625
98-99	0.1307367996506182
100	0.13133159207554135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4038511E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.44138895160898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.08118246209159	59.051995006532366
2	5.863137368577307	7.439311565532522
3	0.6598150966927151	1.2557875855627807
4	0.1523908464733935	0.3867154785513394
5	0.061718075127018955	0.19577402047389164
6	0.04184630584919298	0.1592872659339985
7	0.023489466508547024	0.10431430667241669
8	0.014201778552424401	0.07207844455591797
9	0.012943789732105917	0.07390547990121367
>10	0.06981143667289938	0.8329911877798681
>50	0.008674460228834452	0.39548640425718323
>100	0.008064444609852995	0.9781000078501929
>500	9.466914870088637E-4	0.42094037299014325
>1k	0.0011364286988290904	1.3718324864872238
>5k	2.1378289940625513E-4	0.9876275158999365
>10k+	4.2756579881251026E-4	26.273852871018967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1463554	10.425279433125066	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	341036	2.429288975162679	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	276699	1.970999630943766	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	240853	1.715659160718683	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123327	0.8784906034550245	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114093	0.8127143968473579	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94331	0.6719444818613598	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81513	0.5806385021887293	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81085	0.5775897458070874	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79913	0.5692412820704418	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67662	0.48197419227722943	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49800	0.3547384761816976	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48951	0.34869082625643133	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48522	0.3456349466122155	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46241	0.3293867846810819	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39877	0.28405434166059346	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39805	0.28354146675527053	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38445	0.2738538296547262	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35658	0.2540012968611842	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34650	0.2468210481866631	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28316	0.20170230304339254	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27419	0.19531273651457767	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27290	0.19439383564254073	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22313	0.15894135781209273	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21603	0.15388384138460268	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20869	0.1486553666553383	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19735	0.14057758689650204	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18571	0.13228610926044793	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18274	0.1301705002759908	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17815	0.12690092275455708	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17770	0.12658037593873026	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16357	0.11651520592176763	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15149	0.10791030473246059	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14693	0.10466209699874866	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14351	0.1022259411984647	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015884875539863166	0.0	0.0	7.123262573929671E-6	0.0
2	0.004031766616844194	0.0	0.0	7.123262573929671E-6	0.0
3	0.006859701858694273	0.0	0.0	2.8493050295718684E-5	0.0
4	0.010713386911190226	0.0	0.0	4.273957544357803E-5	0.0
5	0.01775117033423274	0.0	0.0	7.123262573929671E-5	0.0
6	0.0309220828334287	0.0	0.0	7.835588831322638E-5	0.0
7	0.04626559041767321	0.0	0.0	7.835588831322638E-5	0.0
8	0.10184840828204644	0.0	0.0	7.835588831322638E-5	0.0
9	0.31018959204434143	0.0	0.0	7.835588831322638E-5	0.0
10-11	0.7656830557029873	0.0	0.0	7.835588831322638E-5	0.0
12-13	1.33653063348385	0.0	0.0	7.835588831322638E-5	0.0
14-15	1.960781310781464	0.0	0.0	7.835588831322638E-5	0.0
16-17	2.6139809271795276	0.0	0.0	7.835588831322638E-5	0.0
18-19	3.3048412328059578	0.0	0.0	1.0684893860894506E-4	0.0
20-21	4.159949726862058	0.0	0.0	1.0684893860894506E-4	0.0
22-23	5.110392405576347	0.0	0.0	1.0684893860894506E-4	0.0
24-25	6.093894145896242	0.0	0.0	1.0684893860894506E-4	0.0
26-27	7.0893059812397485	0.0	0.0	1.0684893860894506E-4	0.0
28-29	8.144303908014177	0.0	0.0	1.1041056989590989E-4	0.0
30-31	9.279399360801158	0.0	0.0	1.1397220118287473E-4	0.0
32-33	10.519851428687844	0.0	0.0	1.2109546375680441E-4	0.0
34-35	11.665200817949994	0.0	0.0	1.2821872633073407E-4	0.0
36-37	12.589981943241702	0.0	0.0	1.3534198890466375E-4	0.0
38-39	13.315130073267742	0.0	0.0	1.709583017743121E-4	0.0
40-41	13.985760313184212	0.0	0.0	1.9588972078306597E-4	0.0
42-43	14.561847050588199	0.0	0.0	2.0657461464396046E-4	0.0
44-45	15.04501795097785	0.0	0.0	2.2082113979181978E-4	0.0
46-47	15.46773016027127	0.0	0.0	2.528758213745033E-4	0.0
48-49	15.86289315156002	0.0	0.0	2.8493050295718683E-4	0.0
50-51	16.251160824677207	0.0	0.0	3.3479334097469456E-4	0.0
52-53	16.651801604885307	0.0	0.0	3.8821781027916706E-4	0.0
54-55	17.061079340964294	0.0	0.0	4.5945043601846377E-4	0.0
56-57	17.481693749429695	0.0	0.0	4.915051176011473E-4	0.0
58-59	17.914973318751542	0.0	0.0	5.41367955618655E-4	0.0
60-61	18.348947406174346	0.0	0.0	5.805458997752682E-4	0.0
62-63	18.768685653343148	0.0	0.0	6.339703690797407E-4	0.0
64-65	19.170163416903687	0.0	0.0	6.553401568015297E-4	0.0
66-67	19.54526730078425	0.0	0.0	7.550658328365451E-4	0.0
68-69	19.889616498501873	0.0	0.0	8.262984585758418E-4	0.0
70-71	20.214106040163376	0.0	0.0	8.547915088715605E-4	0.0
72-73	20.524712343068295	0.0	0.0	8.797229278803143E-4	0.0
74-75	20.825616762347515	0.0	3.5616312869648354E-6	9.117776094629979E-4	0.0
76-77	21.117015187721833	0.0	7.123262573929671E-6	9.580788161935407E-4	0.0
78-79	21.40168925322636	0.0	7.123262573929671E-6	0.0010221881793589078	0.0
80-81	21.679264987575962	0.0	7.123262573929671E-6	0.0011967081124201846	0.0
82-83	21.953321830214044	0.0	1.0684893860894507E-5	0.0012465709504376923	0.0
84-85	22.226238950840298	0.0	1.4246525147859342E-5	0.001278625632020376	0.0
86-87	22.49865744308638	0.0	1.4246525147859342E-5	0.0012857488945943055	0.0
88	22.703205489528056	0.0	2.1369787721789013E-5	0.0012893105258812705	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	4025	0.0	40.868813	9
GCTTGCT	865	0.0	38.03276	7
CTTGCTG	2545	0.0	36.934223	8
CTGCTGA	3215	0.0	35.96201	9
CCTGCTG	2450	0.0	34.913395	8
GAAAAAA	95500	0.0	33.62511	1
GCCTGCT	815	0.0	33.44616	7
TACCCGA	5565	0.0	32.34402	6
GCTTGCC	355	0.0	31.777235	3
ACCCGAC	6085	0.0	31.203024	7
ACTGACG	515	0.0	31.030989	4
CTACCCG	5465	0.0	29.586088	5
GTACGAC	575	0.0	29.423552	6
CCCGACG	6880	0.0	28.896063	8
GACACGC	670	0.0	28.062685	2
ACTTGCT	820	0.0	27.510805	7
TTACCGC	840	0.0	27.419584	2
CATTGCC	600	0.0	27.4189	3
CAGTGCC	310	0.0	27.292543	3
GCTGAAC	7210	0.0	27.215317	10-11
>>END_MODULE