##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248453_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15447641
Sequences flagged as poor quality	0
Sequence length	100
%GC	28
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.51127055580849	31.0	30.0	34.0	16.0	34.0
2	30.03553992483383	33.0	30.0	34.0	23.0	34.0
3	30.524699272853375	33.0	31.0	34.0	26.0	34.0
4	34.094884843582264	37.0	35.0	37.0	30.0	37.0
5	33.908460327372964	37.0	35.0	37.0	28.0	37.0
6	33.64262640489898	37.0	35.0	37.0	27.0	37.0
7	33.96304704388198	37.0	35.0	37.0	28.0	37.0
8	33.94155722546893	37.0	35.0	37.0	28.0	37.0
9	35.35837743769421	39.0	35.0	39.0	27.0	39.0
10-11	36.15988719572134	39.0	37.0	39.0	31.0	39.0
12-13	36.24645585044344	39.0	37.0	39.0	31.0	39.0
14-15	37.56776290308663	40.0	38.0	41.0	31.0	41.0
16-17	37.40416931620821	40.0	38.0	41.0	31.0	41.0
18-19	36.7533325314849	39.0	36.0	41.0	30.5	41.0
20-21	36.27300553527881	39.0	35.0	41.0	30.0	41.0
22-23	35.91204100354222	38.0	35.0	41.0	29.0	41.0
24-25	35.650275695816596	38.0	35.0	41.0	27.0	41.0
26-27	35.48787219356017	38.0	35.0	41.0	25.0	41.0
28-29	35.33307393018779	38.0	35.0	40.0	25.0	41.0
30-31	35.25452866881099	38.0	35.0	40.0	25.0	41.0
32-33	35.18933214462972	38.0	34.0	40.0	25.0	41.0
34-35	35.10542269852077	38.0	34.0	40.0	25.0	41.0
36-37	34.99699374810692	38.0	34.0	40.0	25.0	41.0
38-39	34.87741616988639	37.0	33.5	40.0	25.0	41.0
40-41	34.74308475319953	37.0	33.0	40.0	25.0	41.0
42-43	34.59125959750101	37.0	33.0	40.0	25.0	41.0
44-45	34.40909790044965	36.0	33.0	40.0	25.0	41.0
46-47	34.22910760290196	35.5	33.0	40.0	25.0	41.0
48-49	34.011653332699794	35.0	33.0	39.5	25.0	41.0
50-51	33.75566424672867	35.0	33.0	39.0	25.0	41.0
52-53	33.46906757478375	35.0	32.0	39.0	25.0	41.0
54-55	33.265199747974464	35.0	31.5	38.5	25.0	40.5
56-57	33.05852265727822	35.0	31.0	37.5	25.0	40.0
58-59	33.10189953922414	35.0	32.0	37.0	25.0	40.0
60-61	32.93450067877678	35.0	31.0	37.0	25.0	40.0
62-63	32.73267144802239	35.0	31.0	36.0	25.0	40.0
64-65	32.5549226254028	35.0	31.0	36.0	25.0	40.0
66-67	32.39339734785395	35.0	31.0	35.0	25.0	39.0
68-69	32.232382374758714	35.0	31.0	35.0	25.0	39.0
70-71	32.07728571631099	35.0	30.0	35.0	25.0	39.0
72-73	32.062456753105536	35.0	31.0	35.0	25.0	38.5
74-75	32.11653743765795	35.0	31.0	35.0	25.0	37.0
76-77	32.009054877699455	35.0	31.0	35.0	25.0	37.0
78-79	31.84039326781351	35.0	31.0	35.0	24.5	36.5
80-81	31.660281139366198	35.0	31.0	35.0	24.0	36.0
82-83	31.472933472495896	35.0	30.0	35.0	24.0	36.0
84-85	31.287546914121062	35.0	30.0	35.0	23.5	36.0
86-87	31.09521596210062	35.0	30.0	35.0	23.0	35.5
88-89	30.889757309870163	35.0	30.0	35.0	20.5	35.0
90-91	30.657843453249594	35.0	29.5	35.0	20.0	35.0
92-93	30.432517366243818	34.0	29.0	35.0	18.5	35.0
94-95	30.17982373489907	34.0	29.0	35.0	17.0	35.0
96-97	29.90477698180583	34.0	28.5	35.0	15.0	35.0
98-99	29.629263102372718	34.0	28.0	35.0	10.5	35.0
100	28.828626972882137	33.0	26.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4638290208579612
1101	2	0.35388622847594675
1101	3	0.2922347538378922
1101	4	0.2514996468596067
1101	5	0.10821773932163126
1101	6	0.12609456297683863
1101	7	0.15712091496725833
1101	8	0.2119513649182494
1101	9	0.3318234136670313
1101	10-11	0.3797538174898136
1101	12-13	0.34320113909210903
1101	14-15	0.4871505721507532
1101	16-17	0.4707523584653046
1101	18-19	0.5176580260294585
1101	20-21	0.5615179147640674
1101	22-23	0.5420219686339394
1101	24-25	0.5807978727010976
1101	26-27	0.5796007783936119
1101	28-29	0.6253837379433094
1101	30-31	0.5929415018916444
1101	32-33	0.6277269191812067
1101	34-35	0.6367531352940787
1101	36-37	0.6683995947444075
1101	38-39	0.6511075958869625
1101	40-41	0.6648882541979333
1101	42-43	0.6866563739330758
1101	44-45	0.6360217892671116
1101	46-47	0.6392148528549839
1101	48-49	0.6322295516744347
1101	50-51	0.6592308559797644
1101	52-53	0.6547388593980585
1101	54-55	0.620224372247641
1101	56-57	0.6262648233484995
1101	58-59	0.6036723627679663
1101	60-61	0.5757284993906779
1101	62-63	0.5813711607013801
1101	64-65	0.5651373610812342
1101	66-67	0.5537213274049364
1101	68-69	0.535980994357935
1101	70-71	0.4905830366363446
1101	72-73	0.5032780698330441
1101	74-75	0.5262722977817944
1101	76-77	0.5660516915714098
1101	78-79	0.5211792742207244
1101	80-81	0.5299781859239054
1101	82-83	0.48485895454445327
1101	84-85	0.46087627479346693
1101	86-87	0.40907120213712744
1101	88-89	0.42354355908911145
1101	90-91	0.4348967558583716
1101	92-93	0.3887960647858293
1101	94-95	0.36660438903264847
1101	96-97	0.37623592410586326
1101	98-99	0.3632051360283093
1101	100	0.31350620685437036
1102	1	0.18007373226205559
1102	2	0.12339613918982906
1102	3	0.16696285204593408
1102	4	0.14590995512620708
1102	5	0.06734877144132412
1102	6	-0.09455310564874253
1102	7	0.03667552223004833
1102	8	0.15524053497865964
1102	9	0.1773393702994639
1102	10-11	0.1956053251365617
1102	12-13	0.20741974324185009
1102	14-15	0.3054858669197955
1102	16-17	0.3089068467176759
1102	18-19	0.3149745973349951
1102	20-21	0.3087274697729967
1102	22-23	0.3352165819601325
1102	24-25	0.2904548761299779
1102	26-27	0.3057766952102128
1102	28-29	0.320816666792048
1102	30-31	0.3415462342269464
1102	32-33	0.356559119682224
1102	34-35	0.32846210624925476
1102	36-37	0.352895973592787
1102	38-39	0.30714401161353067
1102	40-41	0.268286909666557
1102	42-43	0.35918942256481756
1102	44-45	0.3491284592855095
1102	46-47	0.3301975164688997
1102	48-49	0.32715032588694015
1102	50-51	0.3059705530067234
1102	52-53	0.3253424673546803
1102	54-55	0.33174631733837145
1102	56-57	0.29857031739511086
1102	58-59	0.3218900515539005
1102	60-61	0.2971231373845882
1102	62-63	0.2787257153246685
1102	64-65	0.2634786645952474
1102	66-67	0.2816394097493671
1102	68-69	0.2804808006674975
1102	70-71	0.2452589186357983
1102	72-73	0.2631032524159451
1102	74-75	0.2753050199792142
1102	76-77	0.2809483644371653
1102	78-79	0.24446198811974895
1102	80-81	0.2456436454976192
1102	82-83	0.21143070205485515
1102	84-85	0.179622763368549
1102	86-87	0.1730107504636642
1102	88-89	0.18462788221739146
1102	90-91	0.20114221261719933
1102	92-93	0.15786497183053072
1102	94-95	0.19427637275306253
1102	96-97	0.23709112203467697
1102	98-99	0.21378980001450998
1102	100	0.159942207073545
1103	1	0.06386389299029105
1103	2	0.02931120318556424
1103	3	0.1436731557886155
1103	4	0.0737573366278852
1103	5	-0.010982045212109881
1103	6	-0.010717309661082197
1103	7	0.04649078788538219
1103	8	0.05453168659749963
1103	9	0.06372059439038225
1103	10-11	0.15963528017287842
1103	12-13	0.18110673529016452
1103	14-15	0.25845752586270265
1103	16-17	0.29657839209986037
1103	18-19	0.23825004957315343
1103	20-21	0.23544028942691142
1103	22-23	0.26871584717806485
1103	24-25	0.2686995546052131
1103	26-27	0.2407305568276641
1103	28-29	0.2830318640145961
1103	30-31	0.24526471436775665
1103	32-33	0.2585791782176372
1103	34-35	0.2845346568275531
1103	36-37	0.2695751252102596
1103	38-39	0.23238813748704246
1103	40-41	0.24573762805056276
1103	42-43	0.24417823554039586
1103	44-45	0.22642119500010693
1103	46-47	0.27119573331163593
1103	48-49	0.2715272619190685
1103	50-51	0.2633383396246316
1103	52-53	0.28349808481919325
1103	54-55	0.24684755092483357
1103	56-57	0.2878137142397392
1103	58-59	0.24608063560800275
1103	60-61	0.21915405495947482
1103	62-63	0.24073747436856507
1103	64-65	0.27273789080832955
1103	66-67	0.26529476257312723
1103	68-69	0.2435955493861428
1103	70-71	0.2676375227259342
1103	72-73	0.2565488832549292
1103	74-75	0.26801941880940916
1103	76-77	0.24161449353253772
1103	78-79	0.2122870311678433
1103	80-81	0.2436260608923142
1103	82-83	0.2553492431995572
1103	84-85	0.25623956390593605
1103	86-87	0.23305597395327027
1103	88-89	0.29503165823887656
1103	90-91	0.30068923388650504
1103	92-93	0.2913589533392269
1103	94-95	0.21496664844690727
1103	96-97	0.2819887931546887
1103	98-99	0.2877304479479186
1103	100	0.26077208172369026
1104	1	-0.06812303533581598
1104	2	-0.014472464999897738
1104	3	0.021642704826476233
1104	4	0.031733625559610346
1104	5	-0.04100649441906512
1104	6	-0.05534891616075299
1104	7	-0.04598142833717844
1104	8	0.010404009469937137
1104	9	0.04167398956919044
1104	10-11	0.07208540950308873
1104	12-13	0.07296568864950359
1104	14-15	0.08449309949045869
1104	16-17	0.1344226065028451
1104	18-19	0.1279035688852872
1104	20-21	0.09360209080760029
1104	22-23	0.13735281629281104
1104	24-25	0.1089874167065048
1104	26-27	0.08186042176819797
1104	28-29	0.10055444159915083
1104	30-31	0.09992766867672032
1104	32-33	0.12964659226137343
1104	34-35	0.10250195775432047
1104	36-37	0.16981310912252212
1104	38-39	0.12699918569003898
1104	40-41	0.1576559674573872
1104	42-43	0.19131155040361136
1104	44-45	0.15454338108082055
1104	46-47	0.16297616260118986
1104	48-49	0.14458539964115147
1104	50-51	0.15656118144990927
1104	52-53	0.16158376406632868
1104	54-55	0.18416894298816544
1104	56-57	0.20291692319301546
1104	58-59	0.20372068232422436
1104	60-61	0.1820375977108597
1104	62-63	0.14861273314800627
1104	64-65	0.1487852094181079
1104	66-67	0.1679327549024947
1104	68-69	0.19218339301177423
1104	70-71	0.17987520578020622
1104	72-73	0.17807475780272597
1104	74-75	0.1724822522757492
1104	76-77	0.20192986569464466
1104	78-79	0.232393269510748
1104	80-81	0.20094334451486517
1104	82-83	0.19554437848738004
1104	84-85	0.18487449822265845
1104	86-87	0.1887834936553432
1104	88-89	0.15722801327346048
1104	90-91	0.1509699353499947
1104	92-93	0.1113578381624798
1104	94-95	0.11656806356714
1104	96-97	0.14707349481311027
1104	98-99	0.1515611950600686
1104	100	0.13234665147195557
1105	1	0.12151044353166185
1105	2	0.10352829387438334
1105	3	0.10445866442037399
1105	4	0.11924903925448405
1105	5	0.09274718516232383
1105	6	0.04560987833322372
1105	7	0.027261176901347994
1105	8	0.08567759716309098
1105	9	0.14242430932182515
1105	10-11	0.13864248037333482
1105	12-13	0.09795072389771065
1105	14-15	0.16000715552135603
1105	16-17	0.21652723413382802
1105	18-19	0.19789326699557108
1105	20-21	0.21537851429450683
1105	22-23	0.19277619125328016
1105	24-25	0.18323612195445094
1105	26-27	0.20230814603762326
1105	28-29	0.21286685816777862
1105	30-31	0.20231654320650705
1105	32-33	0.2423690927115203
1105	34-35	0.26424582553750753
1105	36-37	0.25299470321459694
1105	38-39	0.23154297754418707
1105	40-41	0.2299554660218206
1105	42-43	0.2296544926054267
1105	44-45	0.2673302196206251
1105	46-47	0.24486936438545115
1105	48-49	0.24339575159270765
1105	50-51	0.24264627977697018
1105	52-53	0.27644254543697144
1105	54-55	0.24619881858390613
1105	56-57	0.22970823980253385
1105	58-59	0.256578479305297
1105	60-61	0.22340966836500797
1105	62-63	0.22871955278316136
1105	64-65	0.2388404416929788
1105	66-67	0.24734798104678646
1105	68-69	0.2527570509601418
1105	70-71	0.2667873668879608
1105	72-73	0.26572708955009006
1105	74-75	0.2129897698695089
1105	76-77	0.22245401991650482
1105	78-79	0.2155038864190466
1105	80-81	0.23296181993482534
1105	82-83	0.198063402753057
1105	84-85	0.20928131222476765
1105	86-87	0.19370865321302233
1105	88-89	0.21982891462908682
1105	90-91	0.19351683906230477
1105	92-93	0.1661953183725018
1105	94-95	0.18832191650837515
1105	96-97	0.22101421980332248
1105	98-99	0.26275767880991197
1105	100	0.30142354127364257
1106	1	0.13393032134167626
1106	2	0.04056408632151687
1106	3	0.17808361861406752
1106	4	0.1327663384924449
1106	5	0.0766245881192873
1106	6	0.08624114237797897
1106	7	0.03439135648568481
1106	8	0.09945965461732698
1106	9	0.15184802123525998
1106	10-11	0.16059455000205247
1106	12-13	0.1567827188187465
1106	14-15	0.18830676053151763
1106	16-17	0.21694428796008935
1106	18-19	0.1768661639449789
1106	20-21	0.18232484688586226
1106	22-23	0.16055793808680363
1106	24-25	0.20350106369262733
1106	26-27	0.20082113613692343
1106	28-29	0.2102260097365516
1106	30-31	0.21600550614826375
1106	32-33	0.23121399368158535
1106	34-35	0.2168331304643445
1106	36-37	0.2085375498882982
1106	38-39	0.19016232440052505
1106	40-41	0.19343344140649776
1106	42-43	0.17937490010148593
1106	44-45	0.1932044550087042
1106	46-47	0.20053335529436822
1106	48-49	0.21759204747219485
1106	50-51	0.1890361782710812
1106	52-53	0.1770718475594535
1106	54-55	0.2092528415887216
1106	56-57	0.1715759805770034
1106	58-59	0.17188184403915585
1106	60-61	0.17409490241378478
1106	62-63	0.1466522224085125
1106	64-65	0.15683533015844375
1106	66-67	0.13389271125338098
1106	68-69	0.15314948875729328
1106	70-71	0.17041062329427348
1106	72-73	0.1336809460060806
1106	74-75	0.11326009847302743
1106	76-77	0.12814081890606133
1106	78-79	0.16825078108248803
1106	80-81	0.18563106241711935
1106	82-83	0.19067277729764243
1106	84-85	0.13211371401062522
1106	86-87	0.1226906330582267
1106	88-89	0.12681603403458297
1106	90-91	0.15004792301884606
1106	92-93	0.13428730233172814
1106	94-95	0.1791004293107541
1106	96-97	0.22685353376607864
1106	98-99	0.24917908373553743
1106	100	0.19638398244399724
1107	1	0.0040801426392746976
1107	2	0.04957703582727646
1107	3	0.11176547828940286
1107	4	0.06436484120662556
1107	5	0.076064488562821
1107	6	0.09070241486409714
1107	7	0.07768377703238372
1107	8	0.10074168330934441
1107	9	0.04563285214344859
1107	10-11	0.106376506122146
1107	12-13	0.1135819288457327
1107	14-15	0.17930355177007584
1107	16-17	0.1465188300496294
1107	18-19	0.13651746777227913
1107	20-21	0.15836431202604473
1107	22-23	0.15040743086317576
1107	24-25	0.12407237674763394
1107	26-27	0.16489230318502024
1107	28-29	0.13489047623455264
1107	30-31	0.15539198688252753
1107	32-33	0.17633105566858376
1107	34-35	0.16563046480047916
1107	36-37	0.17519027580248547
1107	38-39	0.14849102146778392
1107	40-41	0.16551393499428002
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2216	36-37	-0.21489095166969463
2216	38-39	-0.2183380148935541
2216	40-41	-0.25039513625506515
2216	42-43	-0.30243271157731755
2216	44-45	-0.32501913816882677
2216	46-47	-0.31815441382849485
2216	48-49	-0.2428469044097099
2216	50-51	-0.20573076888137365
2216	52-53	-0.23450611537338517
2216	54-55	-0.2533199747312693
2216	56-57	-0.3003124461227671
2216	58-59	-0.2857487901843072
2216	60-61	-0.2823840742145265
2216	62-63	-0.3178988531816458
2216	64-65	-0.351755494321651
2216	66-67	-0.3426266965727578
2216	68-69	-0.41672602600010933
2216	70-71	-0.4209611195099754
2216	72-73	-0.397479774608847
2216	74-75	-0.37725821437812357
2216	76-77	-0.37169644162579374
2216	78-79	-0.33380808774142423
2216	80-81	-0.4107201323927292
2216	82-83	-0.4068922655072278
2216	84-85	-0.4292248850715801
2216	86-87	-0.3946510057650414
2216	88-89	-0.3299065811709525
2216	90-91	-0.35756571227678435
2216	92-93	-0.43356128678519923
2216	94-95	-0.40645921817430164
2216	96-97	-0.3566736053578481
2216	98-99	-0.36218881717661944
2216	100	-0.3328201440402516
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16740.0
1	869.0
2	489.0
3	372.0
4	343.0
5	994.0
6	4690.0
7	10540.0
8	18218.0
9	28662.0
10	39016.0
11	49493.0
12	53666.0
13	56985.0
14	63404.0
15	71092.0
16	77864.0
17	82618.0
18	88130.0
19	93764.0
20	99592.0
21	104497.0
22	108875.0
23	125002.0
24	205236.0
25	313958.0
26	166051.0
27	470171.0
28	214850.0
29	468294.0
30	309884.0
31	374017.0
32	490291.0
33	1037278.0
34	1011757.0
35	3444001.0
36	2055716.0
37	1837950.0
38	1376419.0
39	472084.0
40	3769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.559499110722763	33.64508496205418	24.784085924524824	26.011330002698234
2	12.44094887344955	38.6206408802761	25.274228975011493	23.664181271262862
3	10.789268562357432	41.81549544522353	22.527389465217304	24.86784652720173
4	9.739092622964794	41.52751604397339	22.556639353725462	26.17675197933635
5	9.67585065082094	41.2560275993377	23.1448211343584	25.923300615482965
6	9.431429439175835	38.90114493936593	23.849613140456412	27.817812481001823
7	12.760910007310292	43.52182273677936	20.12749943596222	23.589767819948122
8	11.164389736740057	40.60941970450865	17.845433033656725	30.38075752509456
9	9.072925046542744	39.148449901703486	19.2807256649695	32.497899386784276
10-11	12.497878168399316	52.81211794473574	18.509747333038344	16.180256553826595
12-13	12.14547945122018	53.669919188309144	18.098982411390157	16.085618949080512
14-15	13.16067741325962	52.72916077205121	17.74814832100285	16.36201349368632
16-17	13.231184474303326	52.49739389610054	18.149761187352798	16.12166044224334
18-19	13.589627211379637	51.29179535226699	19.002544477801255	16.116032958552122
20-21	12.722568458730946	51.05237928708735	19.839385440910558	16.38566681327115
22-23	12.915782188066228	51.59968127169805	20.276490481824563	15.20804605841116
24-25	12.948698456402862	51.61875989568969	19.83117687204628	15.60136477586116
26-27	14.12239957691657	51.8269165603929	18.920388549897872	15.130295312792663
28-29	13.69401675073321	52.4562659934083	19.533433921051518	14.316283334806974
30-31	13.895063789286063	52.139987461531945	19.517373667058525	14.447575082123464
32-33	14.268722113151933	52.296735736930366	19.096716497747984	14.337825652169714
34-35	14.326450476408867	52.3440839652905	18.99534003333525	14.33412552496538
36-37	14.730198492822588	52.149781427382	18.77646590414957	14.343554175645831
38-39	14.985482735507693	52.250999257120554	18.749813631902587	14.01370437546916
40-41	15.22501242062637	52.438437399151795	18.660232432709766	13.676317747512073
42-43	15.372248958527557	52.48296098676656	18.79326193897688	13.351528115729005
44-45	15.494465128106436	52.23262984539099	18.825490441758465	13.447414584744111
46-47	15.574601124422333	51.87824785303863	19.07974873357695	13.467402288962091
48-49	15.514540175794675	51.88815489227464	19.352935786771422	13.244369145159263
50-51	15.682633039403976	51.837522491633884	19.515215879172924	12.964628589789221
52-53	15.780740867692309	51.906281135979235	19.575059493602538	12.737918502725917
54-55	15.749627744804744	52.04374600576382	19.518621251414007	12.688004998017426
56-57	15.670461905788985	52.096499468398605	19.280269558612844	12.95276906719957
58-59	15.51019695143	51.870590840677785	19.458024869693116	13.161187338199094
60-61	15.46746757751391	51.58917403929786	19.770651027004295	13.17270735618394
62-63	15.50675645127998	51.179653781721	19.942735879469094	13.370853887529927
64-65	15.370689029110737	51.06746153103544	19.981996846385695	13.579852593468122
66-67	15.099954151196314	50.88207096596313	20.28053842076602	13.737436462074538
68-69	15.000523681418152	50.86268088072845	20.125481974859664	14.01131346299374
70-71	14.810458550351871	50.93245153111792	20.193115618454993	14.063974300075216
72-73	14.79682942120416	51.16927358974647	20.207562638128856	13.826334350920519
74-75	14.658388309717457	51.5640318403064	20.060781349307323	13.716798500668817
76-77	14.263847036716012	52.08067811684002	20.0387377378539	13.616737108590074
78-79	13.87125570451913	52.60572803196294	19.915942919033935	13.607073344483997
80-81	13.504348672844252	53.325609056425726	19.67459525723349	13.495447013496529
82-83	13.154725597480668	53.93644110266593	19.681756701865044	13.22707659798836
84-85	12.830049183825357	54.64487479031484	19.599179561013685	12.925896464846124
86-87	12.576066920062393	55.300063951123	19.393966299848298	12.72990282896631
88-89	12.4723696164924	55.742678687605675	19.195231227420113	12.589720468481817
90-91	12.482922188501837	56.17972350334952	18.933896056788864	12.403458251359783
92-93	12.575366789443713	56.503888325050426	18.593646692137643	12.327098193368224
94-95	12.75558914126456	56.47609369298041	18.352794670281117	12.415522495473917
96-97	12.977431447315555	56.24816210795984	18.307738036712276	12.466668408012325
98-99	13.203314714799387	55.92739581966111	18.267682340912	12.60160712462751
100	13.642459645295894	55.59245785311464	18.067377848263757	12.697704653325708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1978178.0
1	1750568.0
2	959353.0
3	243916.0
4	86728.5
5	76161.5
6	62180.0
7	45699.0
8	34182.0
9	29795.0
10	30478.0
11	34855.0
12	42761.5
13	54282.0
14	68551.5
15	84964.0
16	103119.0
17	122216.5
18	142249.0
19	163376.0
20	184795.0
21	204833.0
22	225123.0
23	246518.0
24	264440.0
25	274197.0
26	278115.5
27	280576.5
28	279091.5
29	272362.5
30	259779.0
31	240051.5
32	215857.0
33	190928.0
34	168890.5
35	150906.5
36	136962.5
37	127595.0
38	121964.0
39	119952.0
40	121822.0
41	127805.5
42	140132.0
43	160064.0
44	187443.0
45	220939.0
46	257084.5
47	291087.5
48	320386.0
49	346525.0
50	374666.5
51	410800.0
52	450843.0
53	480843.0
54	484739.0
55	451022.0
56	380595.5
57	288595.5
58	197653.5
59	123395.5
60	71516.0
61	40279.5
62	23363.0
63	14902.0
64	10744.5
65	8512.5
66	7156.5
67	6273.5
68	5472.5
69	4797.0
70	4338.0
71	3923.5
72	3580.5
73	3378.0
74	3154.5
75	2953.0
76	2807.0
77	2648.0
78	2523.0
79	2414.5
80	2314.5
81	2269.0
82	2198.0
83	2159.0
84	2145.0
85	2103.0
86	2052.5
87	2005.5
88	2012.5
89	1961.0
90	1938.0
91	1929.0
92	1821.5
93	1726.0
94	1702.0
95	1630.5
96	1564.5
97	1482.5
98	1322.5
99	1149.5
100	1019.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14730404467581815
2	0.13846774403936496
3	0.1400666936783422
4	0.13679758611687054
5	0.13525689780077102
6	0.11993416988393243
7	0.12111881678244593
8	0.12233583108255817
9	0.12178558525537976
10-11	0.12140364991651476
12-13	0.12496406409237501
14-15	0.13222730901112992
16-17	0.12583151045522095
18-19	0.13275166091702933
20-21	0.13365147468147404
22-23	0.13405606720145813
24-25	0.13455452518607858
26-27	0.13527308150157036
28-29	0.13576830274603094
30-31	0.1341078550440161
32-33	0.13458365584751744
34-35	0.13612758090377683
36-37	0.13716657449509606
38-39	0.1371147866525381
40-41	0.133881283232825
42-43	0.1315702507586757
44-45	0.13124334000252855
46-47	0.13225643967256878
48-49	0.1334151926498033
50-51	0.1310102947110177
52-53	0.13174827146746873
54-55	0.13415640614641422
56-57	0.13075459223838773
58-59	0.1314731485538795
60-61	0.1329588122872612
62-63	0.12837235148072124
64-65	0.1209084286720542
66-67	0.12084045712869686
68-69	0.1191961931274814
70-71	0.11768463547282074
72-73	0.12079514276645865
74-75	0.12231641064159894
76-77	0.11976262265545917
78-79	0.11502727180156504
80-81	0.11609215931416325
82-83	0.11651940901526647
84-85	0.1124119857523877
86-87	0.11580085269977468
88-89	0.11550307260506637
90-91	0.1205977016167064
92-93	0.11770405591377997
94-95	0.1194972099623496
96-97	0.12273718686238243
98-99	0.12601276790417384
100	0.12632349495952164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.5447641E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.07150674716065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.77195925769396	65.46042023309442
2	4.464060778318963	6.166788083387869
3	0.4653349272071282	0.9642415369283
4	0.11273123281647333	0.31146064432395104
5	0.04902396540957665	0.1693079578780072
6	0.02870190776145945	0.11894904093612173
7	0.015996386155785396	0.07734261460026627
8	0.014752745308898238	0.08151954777141657
9	0.010933339694430975	0.06796640218255996
>10	0.054549505579222266	0.6838991916782661
>50	0.0053465922062120586	0.2562246759454551
>100	0.00469918862382913	0.6514667155417831
>500	5.326674111714902E-4	0.25789395654785835
>1k	8.469836101805848E-4	1.2703189252012659
>5k	1.3961110595348518E-4	0.7205682704997264
>10k+	3.909110966697585E-4	22.74163220348282
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1329313	8.605281544282391	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	319395	2.0675972467252444	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	272736	1.7655511284862198	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	219026	1.4178605005126672	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114038	0.7382227487031838	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108935	0.7051885786315205	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86466	0.5597359493271498	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80692	0.5223580739609368	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80651	0.5220926612678273	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75166	0.4865856217140209	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64200	0.41559743652768727	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53092	0.34369001713594977	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48042	0.3109989415212329	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47459	0.30722490249482104	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47152	0.3052375440366591	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43367	0.2807354210264208	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42361	0.2742230998247564	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39203	0.25377984897499883	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36175	0.23417815056680824	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35422	0.22930361988603956	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28176	0.1823967814891607	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28039	0.18150991468535552	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25523	0.16522263820087482	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24985	0.16173990578885153	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23560	0.15251519633321362	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23013	0.1489742025983126	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20468	0.13249919518455924	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20139	0.13036942015936284	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20041	0.12973501908802773	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19614	0.12697084299149625	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18127	0.11734477775603408	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18051	0.1168527932517334	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15999	0.10356921163561478	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0021686159071148793	0.0	0.0	6.4734803197459085E-6	0.0
2	0.004945738964285874	0.0	0.0	1.2946960639491817E-5	0.0
3	0.008208373045437811	0.0	0.0	2.5893921278983634E-5	0.0
4	0.013277108135798858	0.0	0.0	3.884088191847545E-5	0.0
5	0.021699106031788283	0.0	0.0	5.8261322877713176E-5	0.0
6	0.03695709914542939	0.0	0.0	5.8261322877713176E-5	0.0
7	0.05512168492263641	0.0	0.0	5.8261322877713176E-5	0.0
8	0.11979822679721777	0.0	0.0	5.8261322877713176E-5	0.0
9	0.3606311151327248	0.0	0.0	5.8261322877713176E-5	0.0
10-11	0.88990933955547	0.0	0.0	5.8261322877713176E-5	0.0
12-13	1.5735509389427162	0.0	0.0	6.149806303758614E-5	0.0
14-15	2.3251414245061754	0.0	0.0	7.120828351720499E-5	0.0
16-17	3.112316631387278	0.0	3.2367401598729542E-6	8.091850399682385E-5	0.0
18-19	3.9487323663205274	0.0	6.4734803197459085E-6	1.197593859152993E-4	0.0
20-21	4.990062884035174	0.0	6.4734803197459085E-6	1.4241656703440999E-4	0.0
22-23	6.1480973049542005	0.0	6.4734803197459085E-6	1.4241656703440999E-4	0.0
24-25	7.371837551118647	0.0	9.710220479618863E-6	1.4241656703440999E-4	0.0
26-27	8.634318340256613	0.0	1.2946960639491817E-5	1.553635276739018E-4	0.0
28-29	10.01042165596676	0.0	1.2946960639491817E-5	1.553635276739018E-4	0.0
30-31	11.525096291401386	0.0	1.2946960639491817E-5	1.618370079936477E-4	0.0
32-33	13.18532065834518	0.0	1.2946960639491817E-5	1.6831048831339362E-4	0.0
34-35	14.679652381875005	0.0	1.2946960639491817E-5	1.6831048831339362E-4	0.0
36-37	15.863448017726462	0.0	1.2946960639491817E-5	1.780207087930125E-4	0.0
38-39	16.77175498835065	0.0	1.2946960639491817E-5	2.1362485055161497E-4	0.0
40-41	17.578862041136247	0.0	1.2946960639491817E-5	2.492289923102175E-4	0.0
42-43	18.26242919549982	0.0	1.2946960639491817E-5	3.042535750280577E-4	0.0
44-45	18.840141999674902	0.0	1.2946960639491817E-5	3.2043727582742245E-4	0.0
46-47	19.351126168714046	0.0	1.2946960639491817E-5	3.3662097662678724E-4	0.0
48-49	19.8271179398848	0.0	1.2946960639491817E-5	3.8193533886500856E-4	0.0
50-51	20.29780469393353	0.0	1.2946960639491817E-5	4.175394806236111E-4	0.0
52-53	20.783179774827754	0.0	1.2946960639491817E-5	4.790375436611972E-4	0.0
54-55	21.283139606882372	0.0	1.2946960639491817E-5	5.340621263790374E-4	0.0
56-57	21.793913387811124	0.0	1.2946960639491817E-5	5.858499689370047E-4	0.0
58-59	22.31805166885999	0.0	1.2946960639491817E-5	6.020336697363694E-4	0.0
60-61	22.839163597859375	0.0	1.2946960639491817E-5	6.37637811494972E-4	0.0
62-63	23.342230700467468	0.0	1.2946960639491817E-5	7.250297958115417E-4	0.0
64-65	23.823420676334983	3.2367401598729542E-6	1.2946960639491817E-5	7.735808982096361E-4	0.0
66-67	24.271557061689872	6.4734803197459085E-6	1.2946960639491817E-5	8.027115596484926E-4	0.0
68-69	24.683655582104734	6.4734803197459085E-6	1.2946960639491817E-5	8.383157014070951E-4	0.0
70-71	25.068151182436203	6.4734803197459085E-6	1.2946960639491817E-5	8.642096226860787E-4	0.0
72-73	25.44104954277485	6.4734803197459085E-6	1.2946960639491817E-5	8.933402841249353E-4	0.0
74-75	25.800440986426345	6.4734803197459085E-6	1.2946960639491817E-5	9.354179062032837E-4	0.0
76-77	26.148966693361142	6.4734803197459085E-6	1.2946960639491817E-5	9.87205748761251E-4	0.0
78-79	26.48707657046147	6.4734803197459085E-6	1.2946960639491817E-5	0.0011037283945166774	0.0
80-81	26.81857378741518	6.4734803197459085E-6	1.2946960639491817E-5	0.0012170143001122308	0.0
82-83	27.146021842428887	6.4734803197459085E-6	1.2946960639491817E-5	0.001275275622989944	0.0
84-85	27.470932293157254	6.4734803197459085E-6	1.2946960639491817E-5	0.0013044062844288005	0.0
86-87	27.793441082686996	6.4734803197459085E-6	1.2946960639491817E-5	0.0013205899852281652	3.2367401598729542E-6
88	28.033458312502212	6.4734803197459085E-6	1.2946960639491817E-5	0.0013270634655479113	6.4734803197459085E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	5130	0.0	43.79214	9
GCCTGCT	1025	0.0	40.34839	7
CCTGCTG	3150	0.0	37.747658	8
CTTGCTG	3165	0.0	35.935333	8
GCTTGCT	1155	0.0	35.807007	7
TACCCGA	6430	0.0	34.717175	6
CTGCTGA	4645	0.0	34.40162	9
GAAAAAA	103470	0.0	32.208378	1
CTACCCG	6645	0.0	31.75888	5
ACCCGAC	7585	0.0	30.732185	7
CCCGACG	8565	0.0	29.685879	8
ACTGGCC	675	0.0	27.854137	3
GCTGAAC	9420	0.0	27.565664	10-11
TACCGCC	1650	0.0	27.347702	3
TGAACCG	8495	0.0	27.111368	12-13
TGCCAAA	1770	0.0	27.08249	6
TCGCGAC	635	0.0	26.643452	6
TGAAAAA	16300	0.0	26.303015	1
TACGACG	5310	0.0	25.489737	7
CGTTGCC	535	0.0	25.47873	3
>>END_MODULE