##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248453_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15447641
Sequences flagged as poor quality	0
Sequence length	100
%GC	29
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.506707075857086	34.0	31.0	34.0	31.0	34.0
2	32.800570067623916	34.0	31.0	34.0	31.0	34.0
3	32.92717690681703	34.0	31.0	34.0	31.0	34.0
4	36.3412271168135	37.0	37.0	37.0	35.0	37.0
5	36.25759609509309	37.0	37.0	37.0	35.0	37.0
6	36.16079736705429	37.0	37.0	37.0	35.0	37.0
7	36.19694120286716	37.0	37.0	37.0	35.0	37.0
8	35.81072080843929	37.0	35.0	37.0	35.0	37.0
9	37.59674859093372	39.0	37.0	39.0	34.0	39.0
10-11	37.88147967058531	39.0	38.5	39.0	35.0	39.0
12-13	38.02851034018722	39.0	39.0	39.0	35.0	39.0
14-15	39.602849716665474	41.0	40.0	41.0	37.0	41.0
16-17	39.54511310173508	41.0	39.5	41.0	37.0	41.0
18-19	39.29770603809345	41.0	39.0	41.0	36.0	41.0
20-21	38.84659301701794	40.5	38.0	41.0	35.0	41.0
22-23	38.35301988827938	40.0	36.0	41.0	35.0	41.0
24-25	37.916053234277	40.0	35.0	41.0	35.0	41.0
26-27	37.737287007122966	40.0	35.0	41.0	35.0	41.0
28-29	37.58840757627654	40.0	35.0	41.0	34.5	41.0
30-31	37.53300533071684	40.0	35.0	41.0	34.0	41.0
32-33	37.46852946673217	40.0	35.0	41.0	34.0	41.0
34-35	37.39566934524177	39.0	35.0	41.0	34.0	41.0
36-37	37.321316180250435	39.0	35.0	41.0	33.5	41.0
38-39	37.21439063090604	39.0	35.0	41.0	33.0	41.0
40-41	37.078063084195186	39.0	35.0	41.0	33.0	41.0
42-43	36.90637463674874	38.5	35.0	41.0	33.0	41.0
44-45	36.740429461041984	38.0	35.0	41.0	33.0	41.0
46-47	36.55214686177651	38.0	35.0	41.0	33.0	41.0
48-49	36.34221416072525	37.0	35.0	40.0	33.0	41.0
50-51	36.047466503137926	36.5	35.0	40.0	32.0	41.0
52-53	35.692361150806136	35.5	35.0	39.5	31.5	41.0
54-55	35.44175994250514	35.0	35.0	39.0	31.0	41.0
56-57	35.184078494574024	35.0	35.0	39.0	31.0	41.0
58-59	34.93337180091122	35.0	35.0	38.5	31.0	41.0
60-61	34.657707574897685	35.0	35.0	37.0	30.0	41.0
62-63	34.389263059647746	35.0	35.0	37.0	30.0	40.0
64-65	34.18896807609654	35.0	34.0	36.0	29.0	40.0
66-67	34.00454473922588	35.0	34.0	36.0	29.0	40.0
68-69	33.84436947363031	35.0	34.0	35.0	29.0	39.0
70-71	33.75062936794039	35.0	34.0	35.0	29.0	39.0
72-73	33.64482230652564	35.0	34.0	35.0	29.0	39.0
74-75	33.500186598070215	35.0	34.0	35.0	29.0	37.5
76-77	33.35947614914147	35.0	34.0	35.0	29.0	37.0
78-79	33.21553585430941	35.0	34.0	35.0	29.0	37.0
80-81	33.07679127188416	35.0	34.0	35.0	27.5	36.0
82-83	32.95144135599733	35.0	33.5	35.0	27.0	36.0
84-85	32.829048008042136	35.0	33.0	35.0	27.0	36.0
86-87	32.735512043554095	35.0	33.0	35.0	27.0	36.0
88-89	32.639768266235606	35.0	33.0	35.0	27.0	35.0
90-91	32.55067887064439	35.0	33.0	35.0	27.0	35.0
92-93	32.42223022919811	35.0	33.0	35.0	26.5	35.0
94-95	32.26987819693635	35.0	33.0	35.0	25.0	35.0
96-97	32.13565320426595	35.0	33.0	35.0	25.0	35.0
98-99	31.9647582436697	35.0	33.0	35.0	24.0	35.0
100	31.36835294139733	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22286335548810143
1101	2	0.22244132921808557
1101	3	0.21480394363445043
1101	4	0.13092261359042112
1101	5	0.16279579084604023
1101	6	0.011817354384405121
1101	7	0.08913706510633546
1101	8	-0.03275276914648373
1101	9	-0.05922388897955244
1101	10-11	0.06570867777040235
1101	12-13	0.17336190563394638
1101	14-15	0.25272433964080676
1101	16-17	0.2668959327557374
1101	18-19	0.3095833646248707
1101	20-21	0.30765914497054325
1101	22-23	0.3088708643243905
1101	24-25	0.3230621453056841
1101	26-27	0.3640569619641525
1101	28-29	0.38747962965940275
1101	30-31	0.40799745214421534
1101	32-33	0.3997710907177279
1101	34-35	0.4191545227423319
1101	36-37	0.40719370535170896
1101	38-39	0.40095388290335165
1101	40-41	0.40320887506898373
1101	42-43	0.41065347093910987
1101	44-45	0.43189781946212946
1101	46-47	0.4540993475434476
1101	48-49	0.42420890296921954
1101	50-51	0.4136151021682366
1101	52-53	0.33963003031068695
1101	54-55	0.3923527478537139
1101	56-57	0.3834275064077559
1101	58-59	0.3894154193029067
1101	60-61	0.3948534137398667
1101	62-63	0.348466473006539
1101	64-65	0.3799075276532662
1101	66-67	0.38385014583154486
1101	68-69	0.3698748991944143
1101	70-71	0.4016668335314577
1101	72-73	0.38064554114325944
1101	74-75	0.3978371356254016
1101	76-77	0.382885876420346
1101	78-79	0.4147232338980942
1101	80-81	0.41178421768955786
1101	82-83	0.3805611952439989
1101	84-85	0.38302230771346757
1101	86-87	0.39339597475128585
1101	88-89	0.4603101894362709
1101	90-91	0.3997331615424642
1101	92-93	0.45336381041283147
1101	94-95	0.46334313464817
1101	96-97	0.4479055123440645
1101	98-99	0.46828321992035526
1101	100	0.46181306604225725
1102	1	0.16857865137743744
1102	2	0.16852772473279742
1102	3	0.17203591674552854
1102	4	0.10362983042487173
1102	5	0.11358505406943209
1102	6	-0.024484566265201124
1102	7	0.0219466952584213
1102	8	-0.05059199611596199
1102	9	-0.038141623325515184
1102	10-11	0.06310690753434756
1102	12-13	0.1406878432670453
1102	14-15	0.2080785239635219
1102	16-17	0.24467785489921567
1102	18-19	0.28136135517355854
1102	20-21	0.273157725498578
1102	22-23	0.2390835248948946
1102	24-25	0.2502042281273873
1102	26-27	0.270570091714724
1102	28-29	0.2960846054755564
1102	30-31	0.2695703192898975
1102	32-33	0.28279305052215875
1102	34-35	0.29560033405893904
1102	36-37	0.3114504867793926
1102	38-39	0.28455093515847807
1102	40-41	0.25995841790366114
1102	42-43	0.2847054848204209
1102	44-45	0.3172965358416846
1102	46-47	0.3514874192090218
1102	48-49	0.3322876402783592
1102	50-51	0.29400114639769725
1102	52-53	0.2690465227567316
1102	54-55	0.26082306761460217
1102	56-57	0.22972741386162454
1102	58-59	0.2447032169232557
1102	60-61	0.257816637199511
1102	62-63	0.23550785298392896
1102	64-65	0.24622011036181135
1102	66-67	0.2505644452205331
1102	68-69	0.23145714880131152
1102	70-71	0.2072620526974518
1102	72-73	0.20908272730348898
1102	74-75	0.1651024178225029
1102	76-77	0.1936794778678248
1102	78-79	0.2048995321001641
1102	80-81	0.23095824901571405
1102	82-83	0.1867085392979959
1102	84-85	0.1688125241460554
1102	86-87	0.1923245098954851
1102	88-89	0.21495177086759298
1102	90-91	0.21973501248549354
1102	92-93	0.21754960190468609
1102	94-95	0.18316181180347968
1102	96-97	0.1870530253281757
1102	98-99	0.23522393690407384
1102	100	0.22844246191873552
1103	1	0.11472276345867272
1103	2	0.13374769927757058
1103	3	0.14546269320975824
1103	4	0.09792497598626682
1103	5	0.11768798200236574
1103	6	0.05430844486333086
1103	7	0.08899414375960646
1103	8	-0.004494698313422418
1103	9	-0.12997100677281992
1103	10-11	-0.008767159678995995
1103	12-13	0.09928892070144002
1103	14-15	0.2177815699261032
1103	16-17	0.2536034683265669
1103	18-19	0.24822073531901623
1103	20-21	0.24789560213832118
1103	22-23	0.15987210028566778
1103	24-25	0.16553981692339903
1103	26-27	0.18765842332995675
1103	28-29	0.1993625770543801
1103	30-31	0.18799378557178414
1103	32-33	0.19387023930549674
1103	34-35	0.21716967800637832
1103	36-37	0.21132242541948898
1103	38-39	0.2166546010605117
1103	40-41	0.22668332954137327
1103	42-43	0.2492652866231424
1103	44-45	0.2397280870028382
1103	46-47	0.2662109338094112
1103	48-49	0.25251125494165194
1103	50-51	0.2664685121908619
1103	52-53	0.2101774933851175
1103	54-55	0.22838926685630412
1103	56-57	0.20061168316301092
1103	58-59	0.1974883436649293
1103	60-61	0.22149682913297397
1103	62-63	0.20632038067742542
1103	64-65	0.1847907655513339
1103	66-67	0.1874027994435039
1103	68-69	0.21075191127014392
1103	70-71	0.22001490813253355
1103	72-73	0.21463415668578278
1103	74-75	0.2311519560023143
1103	76-77	0.20629802562426391
1103	78-79	0.20879572485772968
1103	80-81	0.19262759697559773
1103	82-83	0.18958287830071185
1103	84-85	0.1751474833788933
1103	86-87	0.19719182546739944
1103	88-89	0.22266163922459015
1103	90-91	0.2326540422313954
1103	92-93	0.2043051190851557
1103	94-95	0.14313280438172882
1103	96-97	0.1868869917428171
1103	98-99	0.18682502083076358
1103	100	0.15902797790705847
1104	1	-0.2628888535754683
1104	2	-0.22364380108897564
1104	3	-0.18010427311515542
1104	4	-0.21790857718050205
1104	5	-0.10694188847076447
1104	6	-0.04400791237896584
1104	7	0.025849227018355236
1104	8	-0.12650233299012825
1104	9	6.392553903822318E-4
1104	10-11	0.06552406630945029
1104	12-13	0.1132905585507018
1104	14-15	0.15338325134361241
1104	16-17	0.19333828133785147
1104	18-19	0.17995337715398563
1104	20-21	0.16189504914471797
1104	22-23	0.11492331212531326
1104	24-25	0.11065223019129888
1104	26-27	0.1444371058829219
1104	28-29	0.1555674008397503
1104	30-31	0.16058415725693465
1104	32-33	0.19066907494909913
1104	34-35	0.18839345652242656
1104	36-37	0.1439350358244056
1104	38-39	0.16701812504078362
1104	40-41	0.19112013104442838
1104	42-43	0.19260492290260345
1104	44-45	0.1782805207701017
1104	46-47	0.18731067388377198
1104	48-49	0.20935449299896192
1104	50-51	0.20898630715657873
1104	52-53	0.1486932310894602
1104	54-55	0.19346340385761351
1104	56-57	0.19591476801514318
1104	58-59	0.24078514752375213
1104	60-61	0.22649552678011275
1104	62-63	0.17555765960221237
1104	64-65	0.15586934024135957
1104	66-67	0.13366505671817208
1104	68-69	0.1683714113431094
1104	70-71	0.1872166476573227
1104	72-73	0.2041169034597914
1104	74-75	0.1837209673025697
1104	76-77	0.19013811900077826
1104	78-79	0.16699607242041026
1104	80-81	0.17313836168862906
1104	82-83	0.18365461624030388
1104	84-85	0.1872083263479709
1104	86-87	0.17816864482948347
1104	88-89	0.17207913084514104
1104	90-91	0.19318732889778545
1104	92-93	0.19856662907597666
1104	94-95	0.21171267174117503
1104	96-97	0.23044790870000753
1104	98-99	0.23448895583602436
1104	100	0.2548665140759816
1105	1	0.11975728056692958
1105	2	0.12447009892143512
1105	3	0.11465068305201953
1105	4	0.07631449923527356
1105	5	0.0915259182042405
1105	6	0.017804367259003584
1105	7	0.07215921243506074
1105	8	-0.08774663735935917
1105	9	-0.07593200341541717
1105	10-11	0.04031199660905571
1105	12-13	0.09534324357511537
1105	14-15	0.1469849438917521
1105	16-17	0.14770751662164372
1105	18-19	0.1667558069392996
1105	20-21	0.15890810710632763
1105	22-23	0.12620089569710302
1105	24-25	0.12474851758032912
1105	26-27	0.13499147062000105
1105	28-29	0.14382954883604526
1105	30-31	0.15002862691201813
1105	32-33	0.14292973860557368
1105	34-35	0.15691255399736548
1105	36-37	0.14901241211618554
1105	38-39	0.14505152114452358
1105	40-41	0.11842080129961374
1105	42-43	0.12743614487814625
1105	44-45	0.1505406365232318
1105	46-47	0.170943640647792
1105	48-49	0.1314861030834109
1105	50-51	0.14137274055804738
1105	52-53	-0.25752155479824523
1105	54-55	-0.15075609813813884
1105	56-57	-0.08214656767095363
1105	58-59	-0.04628385270097368
1105	60-61	0.00855715358030551
1105	62-63	-0.05971401585028957
1105	64-65	-0.07105667746708377
1105	66-67	-0.06808099414757862
1105	68-69	-0.054828916185250876
1105	70-71	-0.0585360261823098
1105	72-73	-0.07840585325555338
1105	74-75	-0.05089078887633747
1105	76-77	-0.026229862707225493
1105	78-79	-0.03643062298223043
1105	80-81	-0.04417400315949038
1105	82-83	-0.022721956409696986
1105	84-85	-0.0251196078450846
1105	86-87	-0.0042612750902080165
1105	88-89	2.969084203954253E-4
1105	90-91	-0.002741460796876538
1105	92-93	0.011792594828783365
1105	94-95	-0.022409255574252995
1105	96-97	0.02405756702645334
1105	98-99	-0.046315293103525335
1105	100	-0.05774764939386401
1106	1	0.09680505551997953
1106	2	0.10431649300581114
1106	3	0.1052510111894236
1106	4	0.06855493301967641
1106	5	0.07592620275548967
1106	6	0.05685627008836036
1106	7	0.08073724318857245
1106	8	-0.018270998336781474
1106	9	-0.03223946575226222
1106	10-11	0.07010564492794913
1106	12-13	0.116034993610441
1106	14-15	0.1284787407465089
1106	16-17	0.14725281143677904
1106	18-19	0.1161684376539327
1106	20-21	0.12115506526822628
1106	22-23	0.12093655010544069
1106	24-25	0.12955632696356645
1106	26-27	0.14100035055087545
1106	28-29	0.15293614604752293
1106	30-31	0.18932910842206496
1106	32-33	0.16684498914932533
1106	34-35	0.1445302436365239
1106	36-37	0.15164908305666103
1106	38-39	0.1555135103269336
1106	40-41	0.1410722440152199
1106	42-43	0.13056510943000177
1106	44-45	0.14630032157854345
1106	46-47	0.15771397992878633
1106	48-49	0.14415513495304566
1106	50-51	0.17568262798521772
1106	52-53	0.08891104700131791
1106	54-55	0.1293030441029046
1106	56-57	0.13777127193412753
1106	58-59	0.13617608748514698
1106	60-61	0.14211632793767137
1106	62-63	0.10004329387298583
1106	64-65	0.07668173961717883
1106	66-67	0.099164123131402
1106	68-69	0.09512550990785229
1106	70-71	0.08800415529423589
1106	72-73	0.0975406686054967
1106	74-75	0.07590360148358144
1106	76-77	0.11196482800888674
1106	78-79	0.1293987234995697
1106	80-81	0.10204485915346595
1106	82-83	0.120947620637466
1106	84-85	0.12488517289452261
1106	86-87	0.15761177487343758
1106	88-89	0.1327164908692211
1106	90-91	0.13168520218113144
1106	92-93	0.1156848211052548
1106	94-95	0.13416040394329087
1106	96-97	0.10288365903916485
1106	98-99	0.1423707835037753
1106	100	0.13978341880568834
1107	1	0.03450553504996634
1107	2	0.03540714285162494
1107	3	0.05271610904057411
1107	4	0.02910198372445194
1107	5	0.018243785442329852
1107	6	0.011555288753839932
1107	7	7.980881446414401E-4
1107	8	-0.1336564651918124
1107	9	-0.1133550208181262
1107	10-11	-0.012491061268605108
1107	12-13	0.04007431767298897
1107	14-15	0.06492375610991274
1107	16-17	0.05967120258301151
1107	18-19	0.07519207938842243
1107	20-21	0.015248138120810495
1107	22-23	0.03718264251752146
1107	24-25	0.063041822307909
1107	26-27	0.09214930233306973
1107	28-29	0.06795383888837847
1107	30-31	0.06273549706754267
1107	32-33	0.05527600577530478
1107	34-35	0.07161366904354338
1107	36-37	0.05854227591675709
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2216	42-43	-0.4648415667793131
2216	44-45	-0.4908252565739133
2216	46-47	-0.44829609272910886
2216	48-49	-0.4232996542152847
2216	50-51	-0.41530661299209726
2216	52-53	-0.37810181796439934
2216	54-55	-0.37418885518366807
2216	56-57	-0.3585440239448445
2216	58-59	-0.3699466195473633
2216	60-61	-0.3545996415122801
2216	62-63	-0.33023107801462004
2216	64-65	-0.35123311524669276
2216	66-67	-0.4130923775472084
2216	68-69	-0.4199250507553458
2216	70-71	-0.36062464429877394
2216	72-73	-0.3731123815424624
2216	74-75	-0.34098783076161254
2216	76-77	-0.34920491629000594
2216	78-79	-0.3859198186819057
2216	80-81	-0.41251106215705136
2216	82-83	-0.45170449024915627
2216	84-85	-0.4386856668582624
2216	86-87	-0.44425326778954854
2216	88-89	-0.435408712573647
2216	90-91	-0.47201318940044246
2216	92-93	-0.48811114438295533
2216	94-95	-0.4729365993738597
2216	96-97	-0.4647383097060498
2216	98-99	-0.42953499731691025
2216	100	-0.4588626144813155
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	18.0
7	190.0
8	813.0
9	1621.0
10	2758.0
11	4384.0
12	6730.0
13	9473.0
14	12129.0
15	15089.0
16	19467.0
17	24949.0
18	32053.0
19	40044.0
20	49681.0
21	61111.0
22	71282.0
23	82649.0
24	95314.0
25	109227.0
26	126639.0
27	145613.0
28	171238.0
29	207034.0
30	238759.0
31	289716.0
32	369749.0
33	521148.0
34	771199.0
35	3955243.0
36	2029766.0
37	3453886.0
38	1857378.0
39	664034.0
40	7255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.224704845981535	21.89046049290234	32.435954282501704	26.44888037861442
2	15.967100055387373	22.45096378950105	39.48385001026699	22.098086144844586
3	12.726102674145181	19.709343078432497	44.505978523045734	23.058575724376585
4	13.039675918990623	16.28260940213512	49.002427785016145	21.675286893858107
5	11.864139467499712	17.542419512843185	51.03754367811858	19.55589734153853
6	10.747602896496156	13.585270812213615	47.15794602368162	28.50918026760861
7	14.668602150969202	14.205987826879198	45.657909838790275	25.46750018336133
8	9.787856929093575	14.460298501240414	47.99805355393746	27.753791015728552
9	10.61341340079045	12.480870056470112	49.98080936759211	26.92490717514732
10-11	14.566684693105861	19.58138736595007	50.24616351169452	15.60576442924955
12-13	12.846461909679533	24.67844605869515	47.17461097605313	15.300481055572185
14-15	14.197250903108877	23.158214939103633	46.67191844258281	15.97261571520468
16-17	18.38929597627439	23.51214619813555	46.48395643559524	11.614601389994823
18-19	18.362580538244377	19.74230351193031	46.56650243435401	15.328613515471307
20-21	14.122415309304817	18.817528029822004	50.98134681226026	16.07870984861292
22-23	13.94813073374934	19.38884069917412	52.205589784656546	14.45743878241999
24-25	13.881499208323264	20.57326665714199	50.83798815776671	14.707245976768032
26-27	16.800992747181436	20.66278070717635	47.717271232212674	14.818955313429536
28-29	15.28369499074278	22.799843339332185	49.74414788249155	12.172313787433483
30-31	15.183105568477995	20.969356374525475	50.03693851562711	13.810599541369422
32-33	15.825260005516167	21.643111928210963	49.69980060938092	12.831827456891945
34-35	15.664523707787446	21.815079060484237	49.70708227102219	12.813314960706126
36-37	15.911259470176661	21.13884715705754	49.533453859789354	13.416439512976448
38-39	16.28101514464485	21.21022250013894	49.03258775617903	13.476174599037183
40-41	16.417000102248654	21.569367585779222	49.024493164375016	12.98913914759711
42-43	16.424767264618826	21.80491901012326	49.12776485185553	12.642548873402381
44-45	16.64399747662169	21.22824694797782	49.04917253841953	13.078583036980957
46-47	16.939345487322473	20.88126719499149	49.22912611095922	12.950261206726818
48-49	16.4664529589333	20.928130023239245	49.38881029691582	13.216606720911637
50-51	16.447979633704293	20.847041744909866	49.45655795278683	13.248420668599007
52-53	16.240281434748905	21.089927292224075	49.52179127030719	13.148000002719826
54-55	16.040759625083385	21.280512500969813	49.59522523373703	13.08350264020977
56-57	16.060224693556293	21.432817113474524	49.15802871083611	13.348929482133071
58-59	16.126657315466794	21.446545126962345	49.20846784206606	13.218329715504806
60-61	15.862655329561456	21.185401561354606	49.37275828750442	13.579184821579519
62-63	15.838084123872864	21.161224744143585	49.33647121281523	13.66421991916831
64-65	15.795161579275629	21.18526604036586	49.26423823869878	13.75533414165973
66-67	15.585391402356704	21.0273171723114	49.568597594088956	13.818693831242943
68-69	15.584281802566677	20.774300785656063	49.77403072710756	13.867386684669706
70-71	15.505449305086985	20.641943907966613	49.93275714109191	13.919849645854493
72-73	15.486353729955443	20.371373061842228	50.30305981056188	13.839213397640446
74-75	15.4313008895698	20.31113156461328	50.430175306699475	13.827392239117438
76-77	15.400569445605333	20.21703469394322	50.575741917623	13.806653942828447
78-79	15.525528370969575	20.137834793193264	50.63997638904774	13.696660446789425
80-81	15.513162989518445	19.855091854222596	50.83783843191071	13.793906724348243
82-83	15.470800245027702	19.788133196893188	51.0255331283494	13.715533429729716
84-85	15.274592447598556	19.82325635267878	51.218957928478034	13.683193271244631
86-87	15.206164242014914	20.054342308895524	51.19720633296111	13.542287116128456
88-89	15.142995781779007	20.397693007645625	51.08300906022465	13.37630215035072
90-91	15.090738736499084	20.69970638803877	51.01825456825478	13.191300307207355
92-93	14.949374271085675	20.97158839286613	50.84240799870753	13.236629337340666
94-95	14.872446716127005	21.413130882406115	50.67339107666145	13.041031324805427
96-97	14.57219725623288	21.98054456873634	50.777902622110794	12.669355552919983
98-99	14.42179628582341	22.552577988745153	50.5713867586656	12.45423896676584
100	14.412718389749775	23.028460870389956	50.23299114771535	12.325829592144913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426640.0
1	988038.0
2	1485281.0
3	968319.0
4	307584.0
5	71556.0
6	35585.0
7	25120.5
8	22547.5
9	24222.0
10	28790.0
11	36164.5
12	47202.0
13	62369.5
14	80162.0
15	98537.0
16	117944.5
17	140037.0
18	163603.0
19	182346.5
20	196173.0
21	210058.5
22	226099.5
23	244556.5
24	259878.0
25	267606.5
26	270457.0
27	272509.0
28	272109.0
29	266786.0
30	254325.0
31	234178.5
32	209937.0
33	185539.0
34	164314.5
35	145646.0
36	130034.5
37	118030.0
38	107707.0
39	98796.5
40	91199.5
41	84782.0
42	80138.5
43	77349.0
44	77026.5
45	82706.0
46	98609.5
47	127945.5
48	172766.5
49	233038.0
50	309643.0
51	403781.5
52	511126.0
53	613392.5
54	682337.0
55	690029.5
56	623753.0
57	497440.0
58	345391.0
59	207568.0
60	108857.0
61	50888.5
62	22126.5
63	9676.5
64	4536.0
65	2446.5
66	1508.5
67	1018.0
68	747.5
69	584.0
70	415.5
71	296.5
72	226.5
73	183.5
74	143.5
75	114.0
76	81.0
77	54.5
78	40.5
79	31.5
80	31.0
81	27.5
82	19.5
83	18.0
84	14.5
85	10.0
86	9.5
87	8.5
88	8.0
89	5.0
90	5.5
91	4.5
92	3.0
93	3.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007858805108171532
2	4.984579846204349E-4
3	8.415524415669681E-5
4	0.004136553924317635
5	0.012804544072457407
6	0.006331063752711498
7	0.0
8	0.0
9	0.0
10-11	0.011716999378740094
12-13	0.0050298942084425705
14-15	0.0010875446937173127
16-17	1.909676694325043E-4
18-19	7.120828351720499E-5
20-21	7.800543785293819E-4
22-23	1.0033894495606157E-4
24-25	3.107270553478036E-4
26-27	0.002621759529497093
28-29	0.0015601087570587637
30-31	0.002265718111911068
32-33	0.002100644363757547
34-35	0.002495526663262048
36-37	8.642096226860787E-4
38-39	0.0010843079535574397
40-41	2.9130661438856585E-5
42-43	3.2367401598729542E-6
44-45	6.214541106956072E-4
46-47	6.797154335733205E-5
48-49	4.1430274046373814E-4
50-51	0.03153879611780206
52-53	0.03549085585300694
54-55	6.441112918147178E-4
56-57	1.553635276739018E-4
58-59	4.466701420624676E-4
60-61	0.003919692333606148
62-63	0.012726862308620456
64-65	0.016572109618549526
66-67	0.017624050170508235
68-69	0.01839763106871787
70-71	0.016225778421443116
72-73	0.014079819695447351
74-75	0.014953739538613049
76-77	0.01354252082890844
78-79	0.014672143144704101
80-81	0.014698037065983084
82-83	0.013209136592441526
84-85	0.013273871395638983
86-87	0.011969465111210184
88-89	0.010072735377524634
90-91	0.007985037974406577
92-93	0.008386393754230824
94-95	0.009888241188411875
96-97	0.005495984791464276
98-99	0.007959144053127594
100	0.008713304510377991
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.5447641E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.72267139956531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.78292071106105	63.51229922206676
2	5.096190906024068	6.902553242362421
3	0.5949208731419692	1.2086889240160816
4	0.16471579105705958	0.4461997356830682
5	0.07899191738861476	0.26747718322653835
6	0.047680409768816996	0.19374268337821302
7	0.037935769137479536	0.17983781393111087
8	0.028018337540971557	0.15179813331594574
9	0.019110745832574643	0.11648076841980584
>10	0.1258662801393251	1.5953843933820007
>50	0.011830346134360376	0.5609253469406886
>100	0.008323141662626193	1.0712616297807145
>500	0.0010032124756339698	0.47020622349909036
>1k	0.0011031465915318066	1.7435680716691309
>5k	3.7087718982068216E-4	1.8202462662433152
>10k+	0.00101753485412341	19.759330362084974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80654	0.5221120817087864	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63715	0.41245779857261056	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63544	0.41135083343793405	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62630	0.4054340724256862	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58642	0.3796178329105396	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56584	0.36629541041250246	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55293	0.3579381473197105	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55125	0.35685060262599316	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50927	0.32967493224369987	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50390	0.3261986733119963	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50036	0.32390706127880625	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49834	0.32259941825421756	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49707	0.32177728625360985	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49486	0.320346647102946	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46884	0.30350265131096715	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46100	0.2984274427402864	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45200	0.29260131045251503	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44951	0.29098941385289834	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43695	0.28285872257129746	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43352	0.2806383188216246	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42331	0.27402889541516406	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41761	0.27033901163290885	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40979	0.26527675002286755	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39389	0.25498391631447154	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39247	0.25406468210906763	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39198	0.2537474815734001	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38977	0.2523168424227363	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38093	0.24659428582008086	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37372	0.24192690650954407	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37221	0.24094941098126246	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37191	0.24075520657167007	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36037	0.23328481028268327	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35732	0.23131039878516077	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35412	0.2292388850828421	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34993	0.22652649682886858	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34208	0.22144481477786804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33832	0.21901078617764355	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33158	0.21464766044213482	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33059	0.21400678589048	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32330	0.20928761873738522	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32166	0.2082259679649469	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31673	0.20503454216731215	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30984	0.2005743142270072	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30693	0.19869053145396115	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29502	0.1909806163931438	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28997	0.18771150883167212	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28344	0.18348432618287802	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28253	0.18289523947378114	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27652	0.17900467780161386	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27345	0.17701731934345186	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26900	0.17413662060116492	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26692	0.17279013669465776	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26128	0.16913909379432107	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25742	0.16664033039089915	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24652	0.15958423684237613	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23260	0.15057315223728981	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23140	0.1497963345989203	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22753	0.14729109771517865	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22469	0.1454526293043708	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22448	0.14531668621765614	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22431	0.14520663705222045	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22262	0.1441126188781834	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22160	0.14345232388556933	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21610	0.13989190970970908	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21081	0.13646743862056349	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20880	0.13516626907629456	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20685	0.1339039404139441	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19707	0.12757287666123263	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18871	0.12216104711392503	No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18715	0.12115118418404468	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18054	0.11687221369269263	No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17890	0.1158105629202543	No Hit
ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17468	0.11307875422532153	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17461	0.1130334398630833	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17389	0.11256734928006161	No Hit
GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17097	0.11067709302669579	No Hit
GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16242	0.10514226735331303	No Hit
TTTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16136	0.10445607843941997	No Hit
GCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16117	0.1043330823133448	No Hit
GATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15567	0.10077266813748455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015083209145007965	0.0	0.0	0.0	0.0
2	0.0037287246641736433	0.0	0.0	6.4734803197459085E-6	0.0
3	0.005877920130329285	0.0	0.0	1.9420440959237725E-5	0.0
4	0.01001447405464692	0.0	0.0	1.9420440959237725E-5	0.0
5	0.017873279162818452	0.0	0.0	1.9420440959237725E-5	0.0
6	0.0338563020722711	0.0	0.0	1.9420440959237725E-5	0.0
7	0.05157421770741565	0.0	0.0	1.9420440959237725E-5	0.0
8	0.1393157699612517	0.0	0.0	1.9420440959237725E-5	0.0
9	0.5035526136320749	0.0	0.0	1.9420440959237725E-5	0.0
10-11	1.2114956581396474	0.0	0.0	2.5893921278983634E-5	0.0
12-13	1.910392013900375	0.0	0.0	2.5893921278983634E-5	0.0
14-15	2.6094566801494157	0.0	0.0	3.5604141758602496E-5	0.0
16-17	3.255775428753167	0.0	0.0	4.2077622078348405E-5	0.0
18-19	3.9212038912608076	0.0	0.0	5.8261322877713176E-5	0.0
20-21	4.7608952072358495	0.0	0.0	6.473480319745908E-5	0.0
22-23	5.752829833370674	0.0	0.0	6.473480319745908E-5	0.0
24-25	6.919376880910166	0.0	0.0	6.473480319745908E-5	0.0
26-27	8.193170076906888	0.0	3.2367401598729542E-6	6.797154335733203E-5	0.0
28-29	9.633509737829874	0.0	6.4734803197459085E-6	7.120828351720499E-5	0.0
30-31	11.21185752568952	0.0	6.4734803197459085E-6	7.120828351720499E-5	0.0
32-33	12.973495435322455	0.0	6.4734803197459085E-6	7.444502367707795E-5	0.0
34-35	14.590302169761713	0.0	6.4734803197459085E-6	7.76817638369509E-5	0.0
36-37	15.814621792414776	0.0	6.4734803197459085E-6	7.76817638369509E-5	0.0
38-39	16.70927942978478	0.0	6.4734803197459085E-6	1.0033894495606159E-4	0.0
40-41	17.598246877953727	0.0	6.4734803197459085E-6	1.1004916543568044E-4	0.0
42-43	18.340026804092613	0.0	6.4734803197459085E-6	1.3594308671466406E-4	0.0
44-45	18.936629223840715	0.0	6.4734803197459085E-6	1.4241656703440999E-4	0.0
46-47	19.449409783668585	0.0	6.4734803197459085E-6	1.4565330719428293E-4	0.0
48-49	19.913386775365893	0.0	6.4734803197459085E-6	1.5212678751402886E-4	0.0
50-51	20.365805367952298	0.0	6.4734803197459085E-6	1.7478396863313954E-4	0.0
52-53	20.842774634651335	0.0	6.4734803197459085E-6	2.0067788991212318E-4	0.0
54-55	21.320954442170166	0.0	6.4734803197459085E-6	2.4275551199047155E-4	0.0
56-57	21.799729809878414	0.0	6.4734803197459085E-6	2.492289923102175E-4	0.0
58-59	22.262311766566818	0.0	6.4734803197459085E-6	2.6541269310958223E-4	0.0
60-61	22.709030459731686	0.0	6.4734803197459085E-6	2.78359653749074E-4	0.0
62-63	23.153007633981137	0.0	6.4734803197459085E-6	3.042535750280577E-4	0.0
64-65	23.586944440254662	0.0	9.710220479618863E-6	3.3338423646691424E-4	0.0
66-67	23.99341103279135	0.0	1.2946960639491817E-5	3.657516380656438E-4	0.0
68-69	24.369860097085372	0.0	1.2946960639491817E-5	3.851720790248815E-4	0.0
70-71	24.731565809951178	0.0	1.2946960639491817E-5	4.0135577982424635E-4	0.0
72-73	25.089617243176484	0.0	1.2946960639491817E-5	4.2077622078348404E-4	0.0
74-75	25.43873527356054	0.0	1.2946960639491817E-5	4.337231814229758E-4	0.0
76-77	25.780476773120245	0.0	1.2946960639491817E-5	4.499068822223406E-4	0.0
78-79	26.11208080249923	0.0	1.2946960639491817E-5	5.308253862191645E-4	0.0
80-81	26.438418655638102	0.0	1.2946960639491817E-5	6.149806303758613E-4	0.0
82-83	26.76016681123027	0.0	1.2946960639491817E-5	6.34401071335099E-4	0.0
84-85	27.0804422500497	0.0	1.2946960639491817E-5	6.408745516548449E-4	0.0
86-87	27.39405647762011	0.0	1.2946960639491817E-5	6.570582524542096E-4	0.0
88	27.628160183163242	0.0	1.2946960639491817E-5	6.602949926140826E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	4555	0.0	61.2958	1
TATCTCG	3400	0.0	50.59981	9
GACTTTT	3905	0.0	50.554626	1
CCCTTTT	9215	0.0	47.743378	1
TGCCCTA	7885	0.0	47.692852	6
CACTTTT	6575	0.0	46.825047	1
CCTTTTC	7535	0.0	46.660675	1
GGCATAT	1340	0.0	43.49884	5
CTTAGGC	1385	0.0	43.4403	1
GCATATC	1365	0.0	43.391327	6
ACTTTTC	7460	0.0	43.349316	1
ATTTCTT	22040	0.0	43.0334	2
GCTTTTC	4950	0.0	42.540783	1
GCTTTCT	3900	0.0	42.423912	1
GCCCTAC	22470	0.0	42.04691	7
TGCTCTA	7915	0.0	41.929413	6
CCCCTAC	50805	0.0	41.791187	7
CGCCCTA	8150	0.0	40.60505	6
AGGCATA	1470	0.0	40.285286	4
GCCCCTA	6190	0.0	39.944782	6
>>END_MODULE