FastQCFastQC Report
Thu 26 May 2016
SRR1248452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13921551
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA87250.047.7879836
GCCCTAC238900.042.359977
GCCCCTA71050.041.217776
CCCCTAC572100.040.6934177
ATGCCCT33300.039.6682365
GTCCCTA35750.038.525846
TGCTCTA93800.037.9361766
CCCCCTA308700.037.6267476
TTGCCCT38450.037.5338255
GGCATAT11150.037.522865
CCGCCTA44100.036.7740146
CGCCCTA94300.036.3392876
CCCGCTA30350.035.9326976
TTGCTCT33350.034.8162465
GTCACGC3900.033.744582
GCATATC12700.033.3118936
TCGCCCT32200.033.2858625
GGCCCTA40600.033.113156
GCTGCGT12250.033.002225
AGCTTGC2300.032.6966132
Produced by FastQC (version 0.11.5)