##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248452_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13921551
Sequences flagged as poor quality	0
Sequence length	100
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42091409211517	34.0	31.0	34.0	31.0	34.0
2	32.764356931206876	34.0	31.0	34.0	31.0	34.0
3	32.89194199698008	34.0	31.0	34.0	31.0	34.0
4	36.30012582649735	37.0	37.0	37.0	35.0	37.0
5	36.19963256967561	37.0	37.0	37.0	35.0	37.0
6	36.08870599260097	37.0	36.0	37.0	35.0	37.0
7	36.122921433107564	37.0	37.0	37.0	35.0	37.0
8	35.62939998567688	37.0	35.0	37.0	35.0	37.0
9	37.40924621114414	39.0	37.0	39.0	34.0	39.0
10-11	37.722025764226984	39.0	37.5	39.0	35.0	39.0
12-13	37.90698148503712	39.0	38.0	39.0	35.0	39.0
14-15	39.412671440129046	41.0	39.0	41.0	36.0	41.0
16-17	39.392958263055604	41.0	39.0	41.0	36.0	41.0
18-19	39.36928252462675	41.0	39.0	41.0	36.0	41.0
20-21	39.30180983426344	41.0	39.0	41.0	36.0	41.0
22-23	39.2959463352898	41.0	39.0	41.0	36.0	41.0
24-25	39.271027811484515	41.0	39.0	41.0	36.0	41.0
26-27	39.165924220656166	41.0	39.0	41.0	35.5	41.0
28-29	39.04870588772759	41.0	39.0	41.0	35.0	41.0
30-31	38.9984731586301	41.0	39.0	41.0	35.0	41.0
32-33	38.92964821232921	41.0	39.0	41.0	35.0	41.0
34-35	38.85682514828987	41.0	39.0	41.0	35.0	41.0
36-37	38.7787280670092	40.5	38.5	41.0	35.0	41.0
38-39	38.6586008627918	40.0	38.0	41.0	34.0	41.0
40-41	38.5034726015801	40.0	38.0	41.0	34.0	41.0
42-43	38.312185581908224	40.0	38.0	41.0	33.5	41.0
44-45	38.12069599859959	40.0	38.0	41.0	33.0	41.0
46-47	37.89599581971865	40.0	37.0	41.0	33.0	41.0
48-49	37.64332117161371	39.5	36.5	41.0	33.0	41.0
50-51	37.269310581845374	39.0	36.0	41.0	32.5	41.0
52-53	36.846048044503085	38.5	35.0	41.0	32.0	41.0
54-55	36.51129511359761	37.5	35.0	40.0	31.5	41.0
56-57	36.16442517791301	37.0	35.0	40.0	31.0	41.0
58-59	35.823763781779775	36.0	35.0	40.0	31.0	41.0
60-61	35.494156505981266	36.0	35.0	39.0	30.5	41.0
62-63	35.18550364826448	35.0	34.5	39.0	30.0	41.0
64-65	34.92595329356621	35.0	34.0	39.0	30.0	41.0
66-67	34.68540380306763	35.0	34.0	38.0	30.0	41.0
68-69	34.4905557218445	35.0	34.0	37.0	29.5	40.0
70-71	34.3911769600959	35.0	34.0	37.0	30.0	40.0
72-73	34.280772559034546	35.0	34.0	36.0	30.0	39.5
74-75	34.102445984646394	35.0	34.0	36.0	30.0	39.0
76-77	33.92482475551755	35.0	34.0	36.0	29.5	39.0
78-79	33.74640684791515	35.0	34.0	35.5	29.0	37.5
80-81	33.58504167387672	35.0	34.0	35.0	29.0	37.0
82-83	33.43785469736813	35.0	34.0	35.0	29.0	37.0
84-85	33.29282502359112	35.0	34.0	35.0	29.0	36.0
86-87	33.1651192097777	35.0	34.0	35.0	29.0	36.0
88-89	33.03841784582767	35.0	34.0	35.0	29.0	36.0
90-91	32.93617007903789	35.0	33.5	35.0	29.0	36.0
92-93	32.797678972694925	35.0	33.0	35.0	28.5	36.0
94-95	32.62565941826453	35.0	33.0	35.0	27.0	35.5
96-97	32.46909442776886	35.0	33.0	35.0	27.0	35.0
98-99	32.28871553176798	35.0	33.0	35.0	25.5	35.0
100	31.463796095708013	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20235757184844516
1101	2	0.18738516877321842
1101	3	0.18846717423596715
1101	4	0.1054048765992377
1101	5	0.1303459695114455
1101	6	-0.0027422118566420295
1101	7	0.09114357235091575
1101	8	-0.06510940854219172
1101	9	-0.15151972784587997
1101	10-11	0.006323677774702219
1101	12-13	0.13010968024777014
1101	14-15	0.2318858132728039
1101	16-17	0.2909780488533258
1101	18-19	0.33282775385552554
1101	20-21	0.3401331219614292
1101	22-23	0.30240379016562
1101	24-25	0.3068891998668022
1101	26-27	0.2932981396888792
1101	28-29	0.30506076744072885
1101	30-31	0.3332822869103751
1101	32-33	0.3620341160281626
1101	34-35	0.36589959402511596
1101	36-37	0.3458528412246267
1101	38-39	0.3465631358503174
1101	40-41	0.3533431719227025
1101	42-43	0.39012522841591846
1101	44-45	0.3753394907189431
1101	46-47	0.3468102288892325
1101	48-49	0.37494409972364195
1101	50-51	0.36076539101455296
1101	52-53	0.30204638473661305
1101	54-55	0.364530460754942
1101	56-57	0.3451027909393005
1101	58-59	0.33551429154622525
1101	60-61	0.3543709314032881
1101	62-63	0.34216870958227474
1101	64-65	0.32571690801463404
1101	66-67	0.36122023630407796
1101	68-69	0.34875226587762853
1101	70-71	0.315687536905358
1101	72-73	0.31573123306325357
1101	74-75	0.32520130209874765
1101	76-77	0.3051494055097237
1101	78-79	0.3383797286552124
1101	80-81	0.31641793856771017
1101	82-83	0.32088993427181833
1101	84-85	0.29545385127998713
1101	86-87	0.3069860403653806
1101	88-89	0.32798435153816285
1101	90-91	0.32857392007898056
1101	92-93	0.3356638271146366
1101	94-95	0.38503515650933196
1101	96-97	0.35428686348564753
1101	98-99	0.35678323790116195
1101	100	0.3327641381747597
1102	1	0.1421722069157383
1102	2	0.16659103692406063
1102	3	0.1648527956695176
1102	4	0.12054340654924545
1102	5	0.11853151133506401
1102	6	-0.04338261769051144
1102	7	0.004142736626491228
1102	8	-0.12828098116202113
1102	9	-0.14620090671184016
1102	10-11	-0.004278461536166844
1102	12-13	0.12289613481738826
1102	14-15	0.22426768276454823
1102	16-17	0.29974985055952175
1102	18-19	0.3313993284911092
1102	20-21	0.3208840659776939
1102	22-23	0.3066839946381492
1102	24-25	0.3147514355735339
1102	26-27	0.32492968020718394
1102	28-29	0.35076787553352773
1102	30-31	0.35458515906431387
1102	32-33	0.3541641310268062
1102	34-35	0.3648716445328404
1102	36-37	0.353191827857799
1102	38-39	0.37031942468420453
1102	40-41	0.3758253442085788
1102	42-43	0.36503829848292924
1102	44-45	0.3654359857028311
1102	46-47	0.40743301546773125
1102	48-49	0.3769295409028288
1102	50-51	0.34262770994359215
1102	52-53	0.32117880311602676
1102	54-55	0.3200483667003411
1102	56-57	0.2984215802314196
1102	58-59	0.314133518588541
1102	60-61	0.324600566071652
1102	62-63	0.2960960933621877
1102	64-65	0.28152753481852955
1102	66-67	0.26808123703086295
1102	68-69	0.3140628024402048
1102	70-71	0.28801440120428623
1102	72-73	0.275283120002463
1102	74-75	0.3129984474062866
1102	76-77	0.31241085405836344
1102	78-79	0.2777704578008695
1102	80-81	0.29028347391668063
1102	82-83	0.2652039803508046
1102	84-85	0.24629710124393256
1102	86-87	0.26742740671971177
1102	88-89	0.29733887530875336
1102	90-91	0.2902925433237229
1102	92-93	0.30187846148292863
1102	94-95	0.3249724599924946
1102	96-97	0.3474673052835797
1102	98-99	0.3103772762699961
1102	100	0.24993334828985425
1103	1	0.1547796685919991
1103	2	0.15748102092914706
1103	3	0.17072836888835496
1103	4	0.10851356776539944
1103	5	0.10891850836649297
1103	6	0.03425264099936953
1103	7	0.037057134483681864
1103	8	-0.0383135649965638
1103	9	-0.14619205703296956
1103	10-11	0.004373435477880605
1103	12-13	0.10859964936367561
1103	14-15	0.20372227221964323
1103	16-17	0.2515030213612306
1103	18-19	0.2356022845963679
1103	20-21	0.2651951207895067
1103	22-23	0.22842452832208693
1103	24-25	0.24356540756502199
1103	26-27	0.22251449101249676
1103	28-29	0.2561136597645799
1103	30-31	0.2681686916592412
1103	32-33	0.27561209789363517
1103	34-35	0.277314089922406
1103	36-37	0.2620989187213283
1103	38-39	0.26165739738713967
1103	40-41	0.28796610259456656
1103	42-43	0.28830968909146293
1103	44-45	0.2907891136561318
1103	46-47	0.2886900538979802
1103	48-49	0.2835366763922522
1103	50-51	0.33463911721592865
1103	52-53	0.22944027242755283
1103	54-55	0.27282436150391476
1103	56-57	0.2499593907126041
1103	58-59	0.28773254332007525
1103	60-61	0.27611334779916064
1103	62-63	0.24449318212854365
1103	64-65	0.2446057194675788
1103	66-67	0.22853190880143615
1103	68-69	0.24163079677017407
1103	70-71	0.23856726328155986
1103	72-73	0.2148818069531231
1103	74-75	0.21163220508725544
1103	76-77	0.1941063082397676
1103	78-79	0.22124748478255896
1103	80-81	0.20373768574316387
1103	82-83	0.22932797912547187
1103	84-85	0.18238816900946375
1103	86-87	0.2056322526243335
1103	88-89	0.1926706086495642
1103	90-91	0.19967813795609146
1103	92-93	0.17945091435244365
1103	94-95	0.19397996968551467
1103	96-97	0.20731594289078004
1103	98-99	0.23890323020349058
1103	100	0.17719112178143348
1104	1	-0.30405355214933394
1104	2	-0.25029878478108714
1104	3	-0.21322490715301257
1104	4	-0.26022289389683806
1104	5	-0.13854848796464125
1104	6	-0.08207247014873786
1104	7	0.009758952471095483
1104	8	-0.23708426252779446
1104	9	-0.1276011313003096
1104	10-11	0.03500276898761001
1104	12-13	0.11563479931026421
1104	14-15	0.13000454888838675
1104	16-17	0.1866377039511633
1104	18-19	0.1983152004213764
1104	20-21	0.19027063447968828
1104	22-23	0.21120660856541207
1104	24-25	0.1842593307783531
1104	26-27	0.248687236290543
1104	28-29	0.2604121903956269
1104	30-31	0.2727978697627478
1104	32-33	0.25194104536841166
1104	34-35	0.2505951389319918
1104	36-37	0.20752527378277108
1104	38-39	0.2338522726131913
1104	40-41	0.24681847886675712
1104	42-43	0.24702120702659158
1104	44-45	0.24709063088738503
1104	46-47	0.23453276618995744
1104	48-49	0.2330927698398213
1104	50-51	0.23834374013880222
1104	52-53	0.11332264761157518
1104	54-55	0.21568514508935976
1104	56-57	0.2266378248400116
1104	58-59	0.24070364957015755
1104	60-61	0.21923175149890994
1104	62-63	0.15769978675583474
1104	64-65	0.15793450213642046
1104	66-67	0.1786530570718199
1104	68-69	0.22102868215901594
1104	70-71	0.20830665707209306
1104	72-73	0.16553411370816917
1104	74-75	0.17177931252791012
1104	76-77	0.17009702859301967
1104	78-79	0.19530626990778188
1104	80-81	0.17899391214115212
1104	82-83	0.16184022372013374
1104	84-85	0.14635100343800644
1104	86-87	0.14849739702603415
1104	88-89	0.12853145793665988
1104	90-91	0.12411890746158605
1104	92-93	0.13720178447218245
1104	94-95	0.09897783707046415
1104	96-97	0.06066968480634927
1104	98-99	0.13328785676743848
1104	100	0.14489552094931923
1105	1	0.14299643498069514
1105	2	0.14546682861961813
1105	3	0.11886000261092278
1105	4	0.07681639442036925
1105	5	0.10150363984910626
1105	6	0.03134243716713314
1105	7	0.05810811638953339
1105	8	-0.14417106708913252
1105	9	-0.08685061531560478
1105	10-11	0.07288160141163047
1105	12-13	0.12650848088241418
1105	14-15	0.1871986743339349
1105	16-17	0.18720033089365984
1105	18-19	0.18663754100084873
1105	20-21	0.20844752796564592
1105	22-23	0.21504335994088564
1105	24-25	0.18550298710270852
1105	26-27	0.21063305596609183
1105	28-29	0.21096340289434323
1105	30-31	0.22596685991740628
1105	32-33	0.23467083145324352
1105	34-35	0.2638002860498858
1105	36-37	0.2440948397599243
1105	38-39	0.23864410116210877
1105	40-41	0.26675275098553897
1105	42-43	0.26215424795220343
1105	44-45	0.2746560434203218
1105	46-47	0.2653058900124847
1105	48-49	0.23553661450481655
1105	50-51	0.2396327279861694
1105	52-53	0.21005906518335848
1105	54-55	0.21197956750317815
1105	56-57	0.2519847800900621
1105	58-59	0.22867904266433214
1105	60-61	0.22248486996803507
1105	62-63	0.16640056953765026
1105	64-65	0.1752088957419815
1105	66-67	0.15222714573943819
1105	68-69	0.1698327562000017
1105	70-71	0.17462850905916127
1105	72-73	0.16988508380501344
1105	74-75	0.17914335493098577
1105	76-77	0.1881103261565542
1105	78-79	0.2004024791999015
1105	80-81	0.18821137978113
1105	82-83	0.17149719463153446
1105	84-85	0.15975164432510525
1105	86-87	0.17609855925126539
1105	88-89	0.18371956692693914
1105	90-91	0.15675491225754712
1105	92-93	0.15979463965234686
1105	94-95	0.143411971670929
1105	96-97	0.1561975281105532
1105	98-99	0.13431275826378908
1105	100	0.1694646542667968
1106	1	0.099363177662255
1106	2	0.1017607695966447
1106	3	0.1092131615060552
1106	4	0.07930357418317158
1106	5	0.06313904279556226
1106	6	0.028193276790133837
1106	7	0.0450630252574058
1106	8	-0.08399079073358706
1106	9	-0.07614881265821083
1106	10-11	0.05124976330785813
1106	12-13	0.10118036896842142
1106	14-15	0.13411410567567117
1106	16-17	0.1611145108817169
1106	18-19	0.13697373587839934
1106	20-21	0.17185904589678813
1106	22-23	0.15031683373687343
1106	24-25	0.16285651810805035
1106	26-27	0.18434515020296516
1106	28-29	0.17481901828344348
1106	30-31	0.18254676544518844
1106	32-33	0.16461598424972124
1106	34-35	0.14278835451064964
1106	36-37	0.1975566514596494
1106	38-39	0.18368153497303297
1106	40-41	0.1863229044259498
1106	42-43	0.19989040103433808
1106	44-45	0.21527022776886895
1106	46-47	0.2355997233351701
1106	48-49	0.23484988126065076
1106	50-51	0.2332519026681581
1106	52-53	0.13446674589329888
1106	54-55	0.1685464266185548
1106	56-57	0.20300222261646184
1106	58-59	0.1805301481703907
1106	60-61	0.19717062270395758
1106	62-63	0.15743112065747766
1106	64-65	0.15880261985883948
1106	66-67	0.13176576927398997
1106	68-69	0.14734583343186358
1106	70-71	0.16187184853719572
1106	72-73	0.14393120931794812
1106	74-75	0.13888010772951986
1106	76-77	0.14931101931289703
1106	78-79	0.16758352021793144
1106	80-81	0.1398904927731479
1106	82-83	0.10209846547767398
1106	84-85	0.10264789936704233
1106	86-87	0.09668391092750994
1106	88-89	0.10943131901193937
1106	90-91	0.12978477544765354
1106	92-93	0.12889198785404687
1106	94-95	0.1371160791533299
1106	96-97	0.17024315980642513
1106	98-99	0.16993294891685196
1106	100	0.1252213397443782
1107	1	0.04345497203402715
1107	2	0.04190095859313914
1107	3	0.05730759340588065
1107	4	0.021321604557570595
1107	5	0.02468599763189161
1107	6	0.0416159759311725
1107	7	-0.004248006681521588
1107	8	-0.21876956013652205
1107	9	-0.1501144478419647
1107	10-11	-0.024128655069219462
1107	12-13	0.023543094811067533
1107	14-15	0.03986370644881987
1107	16-17	0.07159440641064663
1107	18-19	0.07826061288756847
1107	20-21	0.0350359073479396
1107	22-23	0.031369657050206
1107	24-25	0.07961923594309894
1107	26-27	0.08790509288424886
1107	28-29	0.13067308776397368
1107	30-31	0.09123072109550634
1107	32-33	0.09085859042156841
1107	34-35	0.13068029446455398
1107	36-37	0.11679699140080402
1107	38-39	0.08537706514893273
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2216	40-41	-0.5928685331245802
2216	42-43	-0.6047306289107723
2216	44-45	-0.6237942813618105
2216	46-47	-0.5543101315361554
2216	48-49	-0.531072585927113
2216	50-51	-0.5331147617052991
2216	52-53	-0.5209665042807146
2216	54-55	-0.4997409567391813
2216	56-57	-0.4391553190293962
2216	58-59	-0.4680593412434533
2216	60-61	-0.4643706963661245
2216	62-63	-0.425213070666004
2216	64-65	-0.4537598079229994
2216	66-67	-0.42767364314202894
2216	68-69	-0.45715259126781405
2216	70-71	-0.402511274656149
2216	72-73	-0.3638496559991964
2216	74-75	-0.32443246365133405
2216	76-77	-0.36379865904712716
2216	78-79	-0.3533620645838269
2216	80-81	-0.3857495193124407
2216	82-83	-0.4079167725273791
2216	84-85	-0.3844250665388742
2216	86-87	-0.3878936400196906
2216	88-89	-0.43406770409997364
2216	90-91	-0.4445211213523734
2216	92-93	-0.4484284433580328
2216	94-95	-0.4637783809520215
2216	96-97	-0.4882674992407914
2216	98-99	-0.481416731096747
2216	100	-0.3789538526591478
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	36.0
8	65.0
9	106.0
10	103.0
11	146.0
12	218.0
13	339.0
14	743.0
15	1970.0
16	4481.0
17	8682.0
18	14383.0
19	21280.0
20	29111.0
21	38364.0
22	48855.0
23	60256.0
24	73384.0
25	88806.0
26	105708.0
27	125440.0
28	151220.0
29	179733.0
30	217960.0
31	267068.0
32	338976.0
33	447374.0
34	631582.0
35	1006970.0
36	2138744.0
37	4431998.0
38	2573611.0
39	904353.0
40	9484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.486203169956358	23.225343961729955	30.832752173709398	26.455700694604285
2	17.704963015772396	24.798270298226303	35.680256582007296	21.81651010399401
3	15.47545471993895	24.58250296519005	34.465342042717126	25.47670027215388
4	15.781114862438042	21.83215286258171	35.800445370303855	26.58628690467639
5	14.62447710738261	24.010351097050766	37.51006129162128	23.855110503945347
6	13.05438320038021	18.982298011536205	35.07129959593974	32.89201919214384
7	18.21595165653597	20.108168982033682	31.969160620106194	29.70671874132415
8	12.03064227541888	21.224316169943997	33.7600458454665	32.984995709170626
9	13.269340463573348	17.99137897781648	34.88795178066007	33.8513287779501
10-11	18.5034056806699	29.59111357790939	31.791429555420276	20.114051186000438
12-13	16.068335190140314	36.217186003930365	28.190201877744396	19.52427692818492
14-15	17.56561888733836	34.31798328488398	27.689869223744633	20.426528604033027
16-17	22.96747909810302	34.30805543660505	27.33474536748798	15.389720097803952
18-19	23.06262067402076	29.55988108934321	27.3156940309317	20.06180420570433
20-21	17.683882474940898	28.375032625038525	32.981488731822594	20.95959616819798
22-23	17.195788583583017	29.04270034215684	34.6921150494886	19.069396024771542
24-25	17.15358607472196	30.388798537054512	33.05675040977975	19.40086497844378
26-27	20.51157356617176	30.565911973941585	29.112551449914335	19.80996300997232
28-29	19.19282837838338	33.117838836267936	31.28966736582239	16.399665419526297
30-31	19.24651137198202	30.85978669129284	31.547703536583544	18.345998400141596
32-33	19.866143461640203	31.601801490850583	31.19979369664967	17.33226135085954
34-35	19.564993928687798	31.79075766988037	31.16425672254879	17.47999167888304
36-37	19.765930596264685	30.940331438017505	31.022323753963	18.27141421175481
38-39	20.33679942353505	31.071156880289674	30.380978524324583	18.21106517185069
40-41	20.517999996336613	31.79944581580011	30.177391596169517	17.50516259169376
42-43	20.456043295750597	32.14648640801589	30.294587865964072	17.102882430269442
44-45	20.728041193940435	31.378454063562994	30.26433999582659	17.629164746669982
46-47	21.17017682745806	30.853464671321852	30.472120283845094	17.504238217374994
48-49	20.743865087137408	30.887499502118647	30.599857867220315	17.76877754352363
50-51	20.5849997524631	30.88026061719536	30.812570592027072	17.722169038314473
52-53	20.20571304731821	31.20335230860859	31.01116481708541	17.579769826987786
54-55	20.10587181313911	31.199957648114157	31.304623409415882	17.38954712933085
56-57	20.047542189958367	31.393396824680792	30.83307405267503	17.725986932685807
58-59	19.97510970091703	31.448394740562215	31.18526913215024	17.391226426370515
60-61	19.612815718474593	31.391769389734414	31.377345185747725	17.618069706043272
62-63	19.480421423839235	31.25985911637892	31.538410808501066	17.721308651280783
64-65	19.532524002765516	31.11438977651253	31.55762386154774	17.795462359174213
66-67	19.291686258929673	30.786842045708855	32.14049529480354	17.780976400557936
68-69	19.3060790421502	30.769696372087473	32.19763429260924	17.72659029315309
70-71	19.06080929265695	30.80307291040853	32.539077268362036	17.59704052857249
72-73	18.903929042255292	30.46542896202706	33.05215797406611	17.578484021651526
74-75	18.881691653360434	30.08098614164173	33.41316833556736	17.624153869430472
76-77	19.09370854476986	29.873294156174595	33.51936263558557	17.513634663469965
78-79	19.151658946864476	30.1202712948793	33.39351938925893	17.334550368997302
80-81	18.946968454442924	29.78582045166152	33.71635845700263	17.55085263689293
82-83	18.8240206712703	29.53213596241757	34.01684039698121	17.627002969330924
84-85	18.758790088677298	29.439488233916055	34.01502191447188	17.78669976293476
86-87	18.815741445364058	29.714750760544188	33.71731772065264	17.75219007343911
88-89	18.79017564579251	30.214797521803295	33.53463428423545	17.46039254816875
90-91	18.740231358779088	30.646800379052465	33.519951383931925	17.093016878236526
92-93	18.534366643137727	30.958526759846634	33.33294062560926	17.174165971406378
94-95	18.51481845022869	31.466839451993504	33.03844814753793	16.97989395023988
96-97	18.23480139292377	32.09440943745256	33.14062516780207	16.530164001821593
98-99	18.053411627725882	32.86779768358096	32.95546365879499	16.123327029898167
100	17.914498411788244	33.665829213053414	32.49007767248766	15.929594702670682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6842.0
1	6866.5
2	6110.0
3	4743.0
4	4083.5
5	4295.5
6	5190.5
7	6860.0
8	9899.5
9	15541.5
10	25797.0
11	42832.5
12	68454.5
13	102249.0
14	142125.5
15	181001.5
16	214501.5
17	242778.5
18	264511.5
19	279406.5
20	291568.0
21	305944.0
22	325554.0
23	349509.0
24	368289.5
25	375656.5
26	377620.0
27	377672.5
28	374667.0
29	365918.5
30	346830.5
31	318327.0
32	283916.5
33	249309.5
34	217343.0
35	187768.5
36	161856.5
37	140504.0
38	123059.0
39	107141.0
40	92232.5
41	80150.5
42	72675.0
43	71952.5
44	80281.0
45	99508.5
46	127861.0
47	164004.5
48	212271.0
49	282530.0
50	381356.0
51	505096.0
52	636497.5
53	737887.0
54	769389.5
55	715242.5
56	591189.0
57	429877.5
58	275149.0
59	158428.0
60	82622.0
61	39848.5
62	18101.5
63	8126.0
64	3815.0
65	2012.5
66	1215.0
67	799.5
68	590.0
69	439.0
70	333.0
71	253.0
72	173.0
73	131.5
74	115.0
75	81.0
76	50.5
77	38.5
78	23.0
79	11.5
80	10.5
81	8.5
82	9.5
83	10.5
84	5.5
85	2.0
86	3.5
87	3.5
88	1.0
89	3.5
90	3.5
91	1.0
92	2.0
93	1.5
94	1.5
95	1.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007951700209265476
2	5.459161841952811E-4
3	1.0774661530170022E-4
4	0.003957892335415788
5	0.013410862051218287
6	0.006141557072196912
7	0.0
8	0.0
9	0.0
10-11	0.011299028391304963
12-13	0.00502817538074601
14-15	0.0010056350761492021
16-17	1.975354613864504E-4
18-19	4.669019996407009E-5
20-21	8.009165070759716E-4
22-23	8.260573839797017E-5
24-25	3.447891689654407E-4
26-27	0.0026326089672048755
28-29	0.0016988049679234735
30-31	0.002291411352082825
32-33	0.0021800731829377347
34-35	0.002463805936565545
36-37	8.942969070041118E-4
38-39	0.001073874599173612
40-41	4.669019996407009E-5
42-43	0.0
44-45	6.177472610630813E-4
46-47	1.005635076149202E-4
48-49	4.4176112273697086E-4
50-51	0.031849899483182585
52-53	0.035430678665042424
54-55	6.680290148705414E-4
56-57	1.5443681526577032E-4
58-59	4.525357842671409E-4
60-61	0.0036310609356672973
62-63	0.012161001313718564
64-65	0.016302062895147244
66-67	0.016995232786921513
68-69	0.017659670247948667
70-71	0.015612484557216361
72-73	0.013550932651110499
74-75	0.01443086334274105
76-77	0.012861354313179616
78-79	0.013913679589292889
80-81	0.014136355927583067
82-83	0.012559663790334855
84-85	0.012599170882612146
86-87	0.011378042575859544
88-89	0.00968642071562285
90-91	0.007545854624962406
92-93	0.007937333993891916
94-95	0.00996656191540727
96-97	0.005462753395796201
98-99	0.007894235347771235
100	0.008461700855026857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3921551E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.91865872795738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.02796174192949	80.03056303090258
2	7.4207128382883445	13.048382390952899
3	0.9925532216208651	2.6179184388305843
4	0.227964505949602	0.8016933440268187
5	0.09553386437326172	0.4199604609397882
6	0.05166521783038682	0.2725401992721215
7	0.0361830044463874	0.22268128537718687
8	0.025100020835698462	0.17654081327347143
9	0.017990687979406565	0.14235454410683498
>10	0.09668586503278168	1.545609254570681
>50	0.005354844409101721	0.32318415474555023
>100	0.002244748948072082	0.3145228693324493
>500	2.4920969579073454E-5	0.01266505350968364
>1k	2.4517387082847643E-5	0.07138416015935227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015371850449709232	0.0	0.0	0.0	0.0
2	0.003857328827800868	0.0	0.0	1.436621537356003E-5	0.0
3	0.0064360644873548934	0.0	0.0	2.1549323060340045E-5	0.0
4	0.011004520976146983	0.0	0.0	2.873243074712006E-5	0.0
5	0.0200552366614898	0.0	0.0	2.873243074712006E-5	0.0
6	0.0384942740934541	0.0	0.0	2.873243074712006E-5	0.0
7	0.05945458232347818	0.0	0.0	3.591553843390007E-5	0.0
8	0.15942907510808244	0.0	0.0	3.591553843390007E-5	0.0
9	0.57922425453888	0.0	0.0	3.591553843390007E-5	0.0
10-11	1.4078819235011961	0.0	0.0	3.591553843390007E-5	0.0
12-13	2.247450733039731	0.0	0.0	3.591553843390007E-5	0.0
14-15	3.101899350151431	0.0	0.0	4.309864612068009E-5	7.183107686780015E-6
16-17	3.89167844875905	0.0	0.0	5.746486149424011E-5	7.183107686780015E-6
18-19	4.7140796309261805	0.0	0.0	1.0415506145831021E-4	7.183107686780015E-6
20-21	5.761969338042865	0.0	0.0	1.2929593836204025E-4	7.183107686780015E-6
22-23	6.989343356929124	0.0	0.0	1.3288749220543027E-4	7.183107686780015E-6
24-25	8.406430432930929	0.0	0.0	1.3647904604882028E-4	7.183107686780015E-6
26-27	9.922313972056706	0.0	3.5915538433900074E-6	1.3647904604882028E-4	7.183107686780015E-6
28-29	11.584908894131122	0.0	7.183107686780015E-6	1.3647904604882028E-4	7.183107686780015E-6
30-31	13.353993387662051	0.0	7.183107686780015E-6	1.400705998922103E-4	7.183107686780015E-6
32-33	15.346605417744044	0.0	7.183107686780015E-6	1.508452614223803E-4	7.183107686780015E-6
34-35	17.215865531074805	0.0	7.183107686780015E-6	1.5802836910916034E-4	7.183107686780015E-6
36-37	18.672007163569635	0.0	7.183107686780015E-6	1.6880303063933035E-4	7.183107686780015E-6
38-39	19.76061072505499	0.0	7.183107686780015E-6	2.1190167676001043E-4	7.183107686780015E-6
40-41	20.875217136366487	0.0	7.183107686780015E-6	2.478172151939105E-4	7.183107686780015E-6
42-43	21.797696966379682	0.0	7.183107686780015E-6	2.980989690013706E-4	7.183107686780015E-6
44-45	22.55273855621403	0.0	7.183107686780015E-6	3.2323984590510065E-4	7.183107686780015E-6
46-47	23.202163322175814	0.0	7.183107686780015E-6	3.3401450743527066E-4	7.183107686780015E-6
48-49	23.78600272340345	0.0	7.183107686780015E-6	3.5915538433900073E-4	7.183107686780015E-6
50-51	24.35459238701205	0.0	7.183107686780015E-6	3.986624766162908E-4	7.183107686780015E-6
52-53	24.95640392367201	0.0	7.183107686780015E-6	4.345780150501909E-4	7.183107686780015E-6
54-55	25.556480739825613	0.0	7.183107686780015E-6	5.171837534481611E-4	7.183107686780015E-6
56-57	26.15842516397778	0.0	7.183107686780015E-6	5.351415226651111E-4	7.183107686780015E-6
58-59	26.73406145622711	0.0	7.183107686780015E-6	5.49507738038671E-4	7.183107686780015E-6
60-61	27.286798001170993	0.0	7.183107686780015E-6	5.818317226291812E-4	7.183107686780015E-6
62-63	27.839214897822806	0.0	7.183107686780015E-6	6.033810456895212E-4	7.183107686780015E-6
64-65	28.38461389826464	0.0	7.183107686780015E-6	6.357050302800314E-4	7.183107686780015E-6
66-67	28.900831523728932	0.0	7.183107686780015E-6	7.147192148346114E-4	7.183107686780015E-6
68-69	29.381090512113197	0.0	1.0774661530170023E-5	7.829587378590215E-4	7.183107686780015E-6
70-71	29.84327680155753	0.0	1.436621537356003E-5	7.937333993891917E-4	7.183107686780015E-6
72-73	30.30422400492589	0.0	1.436621537356003E-5	8.045080609193616E-4	7.183107686780015E-6
74-75	30.757718734069215	0.0	1.436621537356003E-5	8.332404916664817E-4	7.183107686780015E-6
76-77	31.206950288800435	0.0	1.436621537356003E-5	9.122546762210619E-4	7.183107686780015E-6
78-79	31.641804853496566	0.0	1.436621537356003E-5	0.0010451421684264921	7.183107686780015E-6
80-81	32.069576874013535	0.0	1.436621537356003E-5	0.0011923958760054823	7.183107686780015E-6
82-83	32.495718329085605	0.0	1.436621537356003E-5	0.0012211283067526024	7.183107686780015E-6
84-85	32.92026154269736	0.0	1.436621537356003E-5	0.0012319029682827725	7.183107686780015E-6
86-87	33.340239891374175	0.0	1.436621537356003E-5	0.0012390860759695526	7.183107686780015E-6
88	33.65426740167098	0.0	1.436621537356003E-5	0.0012498607374997225	7.183107686780015E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCTA	8725	0.0	47.787983	6
GCCCTAC	23890	0.0	42.35997	7
GCCCCTA	7105	0.0	41.21777	6
CCCCTAC	57210	0.0	40.693417	7
ATGCCCT	3330	0.0	39.668236	5
GTCCCTA	3575	0.0	38.52584	6
TGCTCTA	9380	0.0	37.936176	6
CCCCCTA	30870	0.0	37.626747	6
TTGCCCT	3845	0.0	37.533825	5
GGCATAT	1115	0.0	37.52286	5
CCGCCTA	4410	0.0	36.774014	6
CGCCCTA	9430	0.0	36.339287	6
CCCGCTA	3035	0.0	35.932697	6
TTGCTCT	3335	0.0	34.816246	5
GTCACGC	390	0.0	33.74458	2
GCATATC	1270	0.0	33.311893	6
TCGCCCT	3220	0.0	33.285862	5
GGCCCTA	4060	0.0	33.11315	6
GCTGCGT	1225	0.0	33.00222	5
AGCTTGC	230	0.0	32.696613	2
>>END_MODULE