##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248451_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9797740
Sequences flagged as poor quality	0
Sequence length	100
%GC	23
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.565449379142535	33.0	30.0	34.0	16.0	34.0
2	30.031723744455356	33.0	30.0	34.0	22.0	34.0
3	30.512076968770348	33.0	31.0	34.0	25.0	34.0
4	33.972769230455185	37.0	35.0	37.0	28.0	37.0
5	33.88295423230255	37.0	35.0	37.0	28.0	37.0
6	33.630515710765955	37.0	35.0	37.0	26.0	37.0
7	33.917883716040635	37.0	35.0	37.0	28.0	37.0
8	33.88352099565818	37.0	35.0	37.0	28.0	37.0
9	35.300678830015904	39.0	35.0	39.0	27.0	39.0
10-11	36.228628949125	39.0	37.0	39.0	31.0	39.0
12-13	36.356581875003826	39.0	37.0	39.0	31.0	39.0
14-15	37.69785802644283	40.0	38.0	41.0	31.0	41.0
16-17	37.55218101317243	40.0	38.0	41.0	31.0	41.0
18-19	36.98259864009455	40.0	36.0	41.0	30.0	41.0
20-21	36.57671713068524	39.5	35.0	41.0	30.0	41.0
22-23	36.233457307501524	39.0	35.0	41.0	29.0	41.0
24-25	35.972974583934665	39.0	35.0	41.0	27.5	41.0
26-27	35.796487659399006	39.0	35.0	41.0	26.0	41.0
28-29	35.64860472925389	38.0	35.0	41.0	25.0	41.0
30-31	35.553931212708235	38.0	35.0	41.0	25.0	41.0
32-33	35.490119149926414	38.0	35.0	41.0	25.0	41.0
34-35	35.417481480423035	38.0	35.0	41.0	25.0	41.0
36-37	35.315891572954584	38.0	34.5	41.0	25.0	41.0
38-39	35.21482234678609	38.0	34.0	40.5	25.0	41.0
40-41	35.1101732644467	38.0	34.0	40.0	25.0	41.0
42-43	35.00321579262156	38.0	33.5	40.0	25.0	41.0
44-45	34.86356210717982	37.0	33.0	40.0	25.0	41.0
46-47	34.7392532869825	37.0	33.0	40.0	25.0	41.0
48-49	34.59689510029864	36.5	33.0	40.0	25.0	41.0
50-51	34.42233586520973	36.0	33.0	40.0	25.0	41.0
52-53	34.21943953401499	35.0	33.0	40.0	25.0	41.0
54-55	34.08987164386889	35.0	33.0	40.0	25.0	41.0
56-57	33.93462910834539	35.0	32.0	39.0	25.0	41.0
58-59	33.981866685582595	35.0	33.0	39.0	25.0	41.0
60-61	33.83187775956496	35.0	32.0	39.0	25.0	41.0
62-63	33.62767474948305	35.0	32.0	39.0	25.0	41.0
64-65	33.428695444051385	35.0	32.0	38.0	25.0	40.5
66-67	33.23636864215625	35.0	31.0	37.5	25.0	40.0
68-69	33.046654075327574	35.0	31.0	37.0	25.0	40.0
70-71	32.85679881278744	35.0	31.0	36.5	25.0	39.5
72-73	32.777123959198754	35.0	31.0	36.0	25.0	39.0
74-75	32.74507799757903	35.0	31.5	36.0	25.0	39.0
76-77	32.58117775119568	35.0	31.0	35.5	25.0	39.0
78-79	32.37537299418029	35.0	31.0	35.0	25.0	37.5
80-81	32.17167994864121	35.0	31.0	35.0	25.0	37.0
82-83	31.96544340837785	35.0	31.0	35.0	25.0	37.0
84-85	31.764078705905646	35.0	31.0	35.0	24.5	36.0
86-87	31.563388342617788	35.0	30.0	35.0	24.0	36.0
88-89	31.357845737894657	35.0	30.0	35.0	24.0	36.0
90-91	31.1333937724414	35.0	30.0	35.0	23.0	36.0
92-93	30.924525860045275	35.0	30.0	35.0	21.5	35.5
94-95	30.70148401570158	35.0	29.0	35.0	20.0	35.0
96-97	30.46016698748895	35.0	29.0	35.0	19.0	35.0
98-99	30.217420088714334	34.0	29.0	35.0	17.5	35.0
100	29.52729078338474	34.0	28.0	35.0	13.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37836765524012606
1101	2	0.30058474805284874
1101	3	0.2667213880676549
1101	4	0.22447840927541307
1101	5	0.13107842073103626
1101	6	0.21359473118072003
1101	7	0.11726312699346408
1101	8	0.22657369030520869
1101	9	0.29585597300771127
1101	10-11	0.3186521067247341
1101	12-13	0.3039334424398277
1101	14-15	0.41931859723287346
1101	16-17	0.4764151505720804
1101	18-19	0.43369499715353754
1101	20-21	0.4542522855416351
1101	22-23	0.40534928716721197
1101	24-25	0.44427586336224323
1101	26-27	0.47918512984347217
1101	28-29	0.43788638648379674
1101	30-31	0.5000402251052307
1101	32-33	0.511643830272476
1101	34-35	0.5275649837568963
1101	36-37	0.5236041260321116
1101	38-39	0.5005047356285388
1101	40-41	0.51857474399295
1101	42-43	0.5211761526620933
1101	44-45	0.523799576674385
1101	46-47	0.5350531678940769
1101	48-49	0.4808451671324647
1101	50-51	0.4808390490758896
1101	52-53	0.46040533434330655
1101	54-55	0.4378787071900305
1101	56-57	0.48295852517995996
1101	58-59	0.493042313105164
1101	60-61	0.41238540361132436
1101	62-63	0.4216025051547305
1101	64-65	0.39009422401259997
1101	66-67	0.38217185063101766
1101	68-69	0.4185447695916551
1101	70-71	0.39731816823849897
1101	72-73	0.39381691259539764
1101	74-75	0.4202687766862354
1101	76-77	0.39667387994838066
1101	78-79	0.3731614447646834
1101	80-81	0.3663442025989454
1101	82-83	0.3242105755880935
1101	84-85	0.29988441759794426
1101	86-87	0.2944367994842949
1101	88-89	0.32441601278679855
1101	90-91	0.2873209976268605
1101	92-93	0.2514081996327526
1101	94-95	0.1927066974788083
1101	96-97	0.2734923026667282
1101	98-99	0.2373569169562586
1101	100	0.14045069485037942
1102	1	0.10646424094087692
1102	2	0.0684900049460353
1102	3	0.07772236713500291
1102	4	0.026450608256361363
1102	5	0.022037905480615905
1102	6	-0.049530574985659825
1102	7	1.7412381804859933E-4
1102	8	0.055702788033876516
1102	9	0.08338380566449644
1102	10-11	0.12709503273382694
1102	12-13	0.13121451359130276
1102	14-15	0.22664228386759788
1102	16-17	0.22055200851625045
1102	18-19	0.17869042316576866
1102	20-21	0.14748915531315987
1102	22-23	0.1653915989493555
1102	24-25	0.15961089775109372
1102	26-27	0.16570914140415738
1102	28-29	0.16968866580810982
1102	30-31	0.1743992486492374
1102	32-33	0.14308902306272842
1102	34-35	0.20646402953417464
1102	36-37	0.1797225159718181
1102	38-39	0.1796147819068068
1102	40-41	0.16973008014178248
1102	42-43	0.19511704719582212
1102	44-45	0.1504667415636547
1102	46-47	0.19812282339855614
1102	48-49	0.22203428370917777
1102	50-51	0.21910682033750817
1102	52-53	0.1949832983419384
1102	54-55	0.1943638965681913
1102	56-57	0.24040396191886515
1102	58-59	0.22681593008981338
1102	60-61	0.2594853730179807
1102	62-63	0.2174559266675402
1102	64-65	0.22292393831446589
1102	66-67	0.24870285732666986
1102	68-69	0.2307745430098791
1102	70-71	0.2358377941232348
1102	72-73	0.21957458218950876
1102	74-75	0.21613640208702378
1102	76-77	0.20932255611671735
1102	78-79	0.18510001872422066
1102	80-81	0.2245207056160865
1102	82-83	0.24489324012606417
1102	84-85	0.16314669283730154
1102	86-87	0.15601964770414867
1102	88-89	0.15267076144716185
1102	90-91	0.15206166910489927
1102	92-93	0.09033906001190317
1102	94-95	0.16443381894158904
1102	96-97	0.20550581099222853
1102	98-99	0.2013241422848182
1102	100	0.15921993105174437
1103	1	-0.03576422709773652
1103	2	0.030844404006241888
1103	3	0.07376338786199099
1103	4	0.08516289830404844
1103	5	0.0473286303209548
1103	6	-0.058462474330873704
1103	7	0.0420484207967462
1103	8	0.07020379030556256
1103	9	0.10031132921433539
1103	10-11	0.15156031760943023
1103	12-13	0.1576503772549529
1103	14-15	0.21268470574447207
1103	16-17	0.27017699145292795
1103	18-19	0.15795452102095453
1103	20-21	0.15480671104236166
1103	22-23	0.143937229832531
1103	24-25	0.16095806881051544
1103	26-27	0.12957188657175323
1103	28-29	0.17156222521074938
1103	30-31	0.14141917103128776
1103	32-33	0.1815789314809777
1103	34-35	0.1723064264092926
1103	36-37	0.1811160821557536
1103	38-39	0.18326012316600782
1103	40-41	0.18404392401637892
1103	42-43	0.15334016101934367
1103	44-45	0.15331690121279706
1103	46-47	0.1981337953011959
1103	48-49	0.18201395388668828
1103	50-51	0.1804560029124147
1103	52-53	0.22099157888700205
1103	54-55	0.2140200509615724
1103	56-57	0.228943675069047
1103	58-59	0.19995839431309292
1103	60-61	0.245411064003207
1103	62-63	0.22641245775068342
1103	64-65	0.23103928713778998
1103	66-67	0.24161173010984527
1103	68-69	0.22342168331861245
1103	70-71	0.209864854235569
1103	72-73	0.1786295423701887
1103	74-75	0.17968218831374827
1103	76-77	0.1917320091753183
1103	78-79	0.18372976018851972
1103	80-81	0.2308011994324346
1103	82-83	0.2242042498982748
1103	84-85	0.24483982560050066
1103	86-87	0.2740915700548854
1103	88-89	0.24477344402230727
1103	90-91	0.2420734428004181
1103	92-93	0.25291128864629187
1103	94-95	0.2486070084064096
1103	96-97	0.2787730790789844
1103	98-99	0.31924171797626855
1103	100	0.31033322444751477
1104	1	0.0156605357859263
1104	2	0.03228511230382125
1104	3	0.09238332949489347
1104	4	0.0860421903735542
1104	5	0.08349156721382656
1104	6	-0.061854947177778286
1104	7	-0.0052019703821102325
1104	8	0.1127680652366223
1104	9	0.08645734561402207
1104	10-11	0.1522154274836609
1104	12-13	0.15825756485102715
1104	14-15	0.1742336879911477
1104	16-17	0.2362260792375892
1104	18-19	0.2228126928735108
1104	20-21	0.19149624809765697
1104	22-23	0.22617971713356155
1104	24-25	0.22615794858898397
1104	26-27	0.21582484622411613
1104	28-29	0.20654365281856712
1104	30-31	0.20312511236133446
1104	32-33	0.2572110983136042
1104	34-35	0.23305945718509236
1104	36-37	0.23185714239959765
1104	38-39	0.21921132446366443
1104	40-41	0.2687567459646161
1104	42-43	0.2817268694270538
1104	44-45	0.25968861404850685
1104	46-47	0.2884457518378909
1104	48-49	0.285742857759935
1104	50-51	0.289861416781946
1104	52-53	0.23552315654232103
1104	54-55	0.2725952135130214
1104	56-57	0.2413093435604523
1104	58-59	0.28568753564681515
1104	60-61	0.3056428575525416
1104	62-63	0.28852383130470116
1104	64-65	0.256914277386997
1104	66-67	0.24721717403475196
1104	68-69	0.20940638145701485
1104	70-71	0.20800497656785666
1104	72-73	0.18200890900457978
1104	74-75	0.20969466932838543
1104	76-77	0.23598517875652192
1104	78-79	0.2720551361102608
1104	80-81	0.20708317056090664
1104	82-83	0.2351570858876464
1104	84-85	0.20865730108586433
1104	86-87	0.2047791500687417
1104	88-89	0.23298743147705636
1104	90-91	0.26956319299658205
1104	92-93	0.21753122569058192
1104	94-95	0.17699357342010558
1104	96-97	0.2047523741310897
1104	98-99	0.2119942979212972
1104	100	0.18961387979255662
1105	1	0.10658648466666065
1105	2	0.07451840821302724
1105	3	0.08734174167619457
1105	4	0.08698872198539931
1105	5	0.13843697318902315
1105	6	0.11531004511245868
1105	7	0.02006542226301633
1105	8	0.046097665866476234
1105	9	0.06216261350507324
1105	10-11	0.06188069675992125
1105	12-13	0.08032182323351122
1105	14-15	0.1522914113376217
1105	16-17	0.14375898771159257
1105	18-19	0.14818023201107167
1105	20-21	0.13544920373534097
1105	22-23	0.12208932141196271
1105	24-25	0.11753524173382601
1105	26-27	0.0986912302194014
1105	28-29	0.101708690634851
1105	30-31	0.11026775374098463
1105	32-33	0.12304069543731089
1105	34-35	0.1390634352113409
1105	36-37	0.1502850517587646
1105	38-39	0.10872494133358401
1105	40-41	0.11857469575888757
1105	42-43	0.06998060717400278
1105	44-45	0.09732923906705082
1105	46-47	0.1090278788121708
1105	48-49	0.10236449464535013
1105	50-51	0.118704086674704
1105	52-53	0.09956140121801127
1105	54-55	0.10748539165899018
1105	56-57	0.08896988783824611
1105	58-59	0.09199845301695575
1105	60-61	0.07801491293766105
1105	62-63	0.11936522366350744
1105	64-65	0.11194780105584101
1105	66-67	0.09063799287554986
1105	68-69	0.09796926472663614
1105	70-71	0.10146873768088227
1105	72-73	0.09296795919768641
1105	74-75	0.13164806857521683
1105	76-77	0.13253034053091284
1105	78-79	0.12640857954540508
1105	80-81	0.10126090291333867
1105	82-83	0.08094932037564817
1105	84-85	0.09373683302210623
1105	86-87	0.08074944530077488
1105	88-89	0.0827531449989678
1105	90-91	0.09752526851348975
1105	92-93	0.08223984664975958
1105	94-95	0.06508953971616549
1105	96-97	0.08912551351055953
1105	98-99	0.06693025346803694
1105	100	0.13487492684177482
1106	1	0.024085671926187047
1106	2	0.03533419265976079
1106	3	0.0541092808360375
1106	4	0.07219763129483425
1106	5	0.07222470223884159
1106	6	0.02539615743031476
1106	7	0.07414930483662374
1106	8	0.09770726759853687
1106	9	0.10784361898353723
1106	10-11	0.044287468191406276
1106	12-13	0.055151848918271185
1106	14-15	0.0736766524034067
1106	16-17	0.07790013077559621
1106	18-19	0.09711128181618989
1106	20-21	0.11080742907513752
1106	22-23	0.09129967489477053
1106	24-25	0.038198600241599934
1106	26-27	0.0876512639235898
1106	28-29	0.10452186169688815
1106	30-31	0.0764551117410548
1106	32-33	0.07717599918096596
1106	34-35	0.10001180158359091
1106	36-37	0.08678246346155305
1106	38-39	0.06415325566432273
1106	40-41	0.06325049345196732
1106	42-43	0.054117266539776665
1106	44-45	0.07633049217547949
1106	46-47	0.08537512551118454
1106	48-49	0.06576352410890962
1106	50-51	0.10602861669065788
1106	52-53	0.10586571260259348
1106	54-55	0.1153674585767206
1106	56-57	0.06772719122435689
1106	58-59	0.10982594027326797
1106	60-61	0.13002745043160502
1106	62-63	0.14443871630499672
1106	64-65	0.10370756901644995
1106	66-67	0.06618902846592079
1106	68-69	0.09417922309693694
1106	70-71	0.10480385183748808
1106	72-73	0.010761194592269874
1106	74-75	0.05019279036730495
1106	76-77	0.05605669804225499
1106	78-79	0.13245820401092345
1106	80-81	0.09651340850760448
1106	82-83	0.10633246172749367
1106	84-85	0.07662663541645287
1106	86-87	0.03765822207726899
1106	88-89	0.021904165059194014
1106	90-91	0.09983070387435689
1106	92-93	0.08922896562357252
1106	94-95	0.07345283105318146
1106	96-97	0.10012881309602761
1106	98-99	0.053116564081094
1106	100	0.0716663560602342
1107	1	0.028803761691843732
1107	2	0.0030623064961154967
1107	3	0.06177135990430571
1107	4	0.04804761556570014
1107	5	0.07128363484955713
1107	6	-0.02388235231504865
1107	7	-0.06595651793900714
1107	8	0.014099154046014917
1107	9	-0.03923679355340681
1107	10-11	-0.05968374394532816
1107	12-13	-0.014252659469420337
1107	14-15	0.026970191158966372
1107	16-17	0.01772610111336803
1107	18-19	-0.006069501912548958
1107	20-21	-2.2227556677023586E-5
1107	22-23	-0.0020705542302152935
1107	24-25	-0.016835136582791677
1107	26-27	0.004767449399643908
1107	28-29	0.030149046134582136
1107	30-31	0.06938113080366293
1107	32-33	0.016976444169600313
1107	34-35	0.04066102849074582
1107	36-37	0.04675897225607173
1107	38-39	0.01746417925227206
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2216	30-31	0.15875579314992194
2216	32-33	0.12416882734506629
2216	34-35	0.10668085838641872
2216	36-37	0.11487607452410487
2216	38-39	0.13906948244265038
2216	40-41	0.10563636687891886
2216	42-43	0.01859254204232741
2216	44-45	0.06354744437479809
2216	46-47	0.03408501849212797
2216	48-49	0.027367454261188584
2216	50-51	0.06506081852171519
2216	52-53	0.055222256763087785
2216	54-55	0.0168133135173818
2216	56-57	-0.02311675740826047
2216	58-59	-0.06670446404891095
2216	60-61	-0.060347995035073154
2216	62-63	-0.08660471700505923
2216	64-65	-0.08327538883295915
2216	66-67	-0.08284019630146844
2216	68-69	-0.12661938352663782
2216	70-71	-0.12909448918478716
2216	72-73	-0.10131880702785168
2216	74-75	-0.08507196816341889
2216	76-77	-0.09735046226722233
2216	78-79	-0.08879183918340772
2216	80-81	-0.15465774532448506
2216	82-83	-0.19213787293776008
2216	84-85	-0.176420223167284
2216	86-87	-0.2021705526006734
2216	88-89	-0.17957923549870003
2216	90-91	-0.16632171774137916
2216	92-93	-0.2449010739808024
2216	94-95	-0.23902390273773122
2216	96-97	-0.20830106490651445
2216	98-99	-0.19734717655614276
2216	100	-0.10837419397956438
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	11218.0
1	536.0
2	318.0
3	255.0
4	209.0
5	462.0
6	2097.0
7	5611.0
8	7906.0
9	11187.0
10	16998.0
11	22332.0
12	25207.0
13	28052.0
14	32562.0
15	37362.0
16	42589.0
17	46366.0
18	50211.0
19	54155.0
20	60186.0
21	64011.0
22	68787.0
23	80411.0
24	178798.0
25	218088.0
26	117147.0
27	297713.0
28	124472.0
29	258983.0
30	170819.0
31	204011.0
32	270950.0
33	565347.0
34	553699.0
35	1907715.0
36	1014704.0
37	1371095.0
38	1381762.0
39	489130.0
40	4279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.646209970456974	33.19817951745539	26.77704608787714	25.3785644242105
2	11.516708854678527	37.4958872437864	28.121133510875612	22.86627039065946
3	9.90967629038142	40.110536587250586	26.284958951248584	23.69482817111941
4	8.585661287922273	40.32406567006988	26.286497454540697	24.80377558746715
5	8.488292667421033	39.66595771363824	26.956459436313352	24.889290182627377
6	8.401007462770457	37.651560379807655	27.737713448663282	26.2097187087586
7	10.711915164584912	43.14430091894405	23.57354480257179	22.57023911389925
8	10.673349609764223	39.18816446621448	20.884680908032717	29.253805015988576
9	8.242889966409548	38.09680946398656	22.87975917968536	30.780541389918533
10-11	9.79221445212393	54.83940954701845	22.033752287758503	13.334623713099116
12-13	9.40981169206317	55.07313479770875	22.546859812341026	12.970193697887055
14-15	9.84487748295857	54.06552390573365	22.543880704437917	13.545717906869866
16-17	9.777894489036424	53.80530569177405	22.935407727767206	13.48139209142232
18-19	9.816924862001835	53.276840301315474	23.55240225273716	13.353832583945529
20-21	9.531563434130007	53.182247941755136	23.821961020353275	13.46422760376159
22-23	9.778272237997763	53.43849357481367	24.0429550107919	12.740279176396669
24-25	9.842404780632089	53.21760675158548	23.88297420827667	13.057014259505761
26-27	10.522565236906663	53.26068653235344	23.409211829958263	12.807536400781638
28-29	10.34824721420371	53.6120053772659	23.674197581559074	12.365549826971318
30-31	10.499169329569828	53.56671112206101	23.713112409596334	12.221007138772835
32-33	10.649036697463375	53.57476421067737	23.55494909722519	12.221249994634068
34-35	10.658729155690777	53.61030843422038	23.536100222455477	12.194862187633367
36-37	10.947016512852256	53.43328851026181	23.389379548659335	12.230315428226596
38-39	11.139182944409031	53.5100594814982	23.48102271442079	11.86973485967198
40-41	11.204236064500085	53.83442353306479	23.413129023985825	11.548211378449297
42-43	11.319715402616135	53.783714532642804	23.52184254463928	11.37472752010178
44-45	11.386233811403143	53.571332389059734	23.518023703526094	11.524410096011028
46-47	11.360006434871607	53.39296900335838	23.700104321369917	11.5469202404001
48-49	11.339720963224199	53.387055314203835	23.88367658880327	11.389547133768703
50-51	11.493280342197963	53.38783301128607	23.985121845474954	11.133764801041018
52-53	11.592777501368532	53.421290071220916	24.06011771627002	10.925814711140529
54-55	11.591944399288408	53.4729313253084	23.94778073595953	10.987343539443655
56-57	11.518583142091543	53.516266573238056	23.76543519013832	11.199715094532076
58-59	11.368766721746812	53.24541462266694	23.980401109847456	11.405417545738793
60-61	11.373679830148273	53.04437430543937	24.272697344651196	11.309248519761168
62-63	11.405711044452733	52.6640162296682	24.403380840202153	11.526891885676909
64-65	11.330234267392953	52.537736653110976	24.42577213158413	11.706256947911942
66-67	11.131355158820593	52.325366431627465	24.70246751306396	11.840810896487982
68-69	11.015498325503106	52.404898218293205	24.559601159943515	12.020002296260177
70-71	10.875843277163908	52.545221402153686	24.578386965706457	12.000548354975948
72-73	10.936765481353701	52.68999378661097	24.608878401523913	11.76436233051142
74-75	10.883549560268108	52.936066395187744	24.43911790161578	11.741266142928373
76-77	10.591960353970435	53.32818756854285	24.40597304543757	11.673879032049147
78-79	10.241923930035563	53.81456518091463	24.28685047015671	11.656660418893088
80-81	9.932293161978773	54.41324291448474	24.096083592216328	11.558380331320159
82-83	9.662288738856763	54.785946021952725	24.172761920489332	11.379003318701182
84-85	9.405147750224858	55.366972683309044	24.053619298565792	11.174260267900298
86-87	9.257750933226703	55.899031510487305	23.787336668402006	11.055880887883987
88-89	9.216347082138391	56.25482552293711	23.641308249308583	10.88751914561592
90-91	9.222156823981654	56.593233566128696	23.490476295346312	10.694133314543333
92-93	9.33652522392013	56.85797299551015	23.15403849235287	10.651463288216847
94-95	9.522705113178876	56.75819960657872	22.946011926496094	10.773083353746317
96-97	9.749929650740134	56.47237462361805	22.9609713496731	10.816724375968723
98-99	9.97199614991774	56.229116686558214	22.90963487378185	10.889252289742196
100	10.311094459285622	55.94155904215074	22.80598316986916	10.941363328694464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1127458.0
1	991655.0
2	544087.5
3	165442.0
4	98980.5
5	92315.5
6	72189.5
7	48000.5
8	31511.0
9	26104.0
10	29048.5
11	37955.5
12	52352.0
13	70424.5
14	91081.0
15	112083.0
16	131541.5
17	149332.0
18	165252.0
19	180464.5
20	195552.0
21	211283.5
22	229991.0
23	249696.0
24	265052.5
25	274348.5
26	278467.0
27	279000.0
28	276904.5
29	270182.0
30	257458.5
31	238003.5
32	214564.5
33	191432.0
34	169056.0
35	149580.0
36	133076.5
37	119198.5
38	107839.5
39	98316.5
40	91314.5
41	87165.5
42	86327.0
43	90171.5
44	98718.0
45	110809.5
46	125295.5
47	137700.0
48	142830.0
49	140146.0
50	131566.5
51	119487.5
52	106637.0
53	94654.0
54	83376.0
55	71219.5
56	57753.0
57	43877.0
58	31106.0
59	20761.0
60	13362.0
61	8547.5
62	5635.5
63	4048.0
64	3156.0
65	2628.0
66	2265.5
67	1980.0
68	1778.5
69	1583.0
70	1457.5
71	1341.0
72	1206.0
73	1106.5
74	1008.0
75	937.0
76	875.0
77	806.5
78	724.0
79	664.5
80	646.5
81	617.5
82	590.5
83	579.5
84	520.5
85	484.5
86	479.5
87	457.5
88	457.0
89	466.0
90	453.5
91	414.0
92	402.0
93	387.0
94	383.0
95	364.0
96	319.0
97	288.5
98	257.5
99	236.5
100	235.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.153698710110699
2	0.1444720925437907
3	0.1466970954526248
4	0.14333917821865041
5	0.1411447946159012
6	0.126212779681845
7	0.12744775836060152
8	0.1289072786173138
9	0.1277743642921735
10-11	0.127651887067834
12-13	0.13128537805657223
14-15	0.13850643107492136
16-17	0.13174977086552614
18-19	0.1392412944209583
20-21	0.140200699344951
22-23	0.1406701953715857
24-25	0.14131320079936802
26-27	0.1420582705807666
28-29	0.14245632155986993
30-31	0.14059875032405433
32-33	0.14078756937824435
34-35	0.1427318953146338
36-37	0.14378826137456188
38-39	0.14400769973483682
40-41	0.14036910552841778
42-43	0.13811348331349882
44-45	0.13778177416424606
46-47	0.1383074055853697
48-49	0.1400833253382923
50-51	0.13739903283818514
52-53	0.13815430905494533
54-55	0.14048647953507645
56-57	0.13719490413095264
58-59	0.13791445782394715
60-61	0.13959341644093431
62-63	0.13462288241982334
64-65	0.12682516580354244
66-67	0.12678434006209596
68-69	0.12516662005727852
70-71	0.12357951935854596
72-73	0.12676392719137272
74-75	0.1283255118017012
76-77	0.12601375419229333
78-79	0.1209870847766934
80-81	0.12195159291836688
82-83	0.12231392137370455
84-85	0.11800680565109913
86-87	0.12180870282330414
88-89	0.12128817461986131
90-91	0.12649345665428968
92-93	0.12357951935854596
94-95	0.12554936138333941
96-97	0.12894300114107946
98-99	0.13282654979617747
100	0.1329592334558786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9797740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.22979875920684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.09958683140871	63.521660740723206
2	5.656428332681354	7.718739336694493
3	0.6812757445739246	1.3944992085542305
4	0.1948232059914098	0.5317099255366956
5	0.09726395564132537	0.3318150059966025
6	0.05292274727575688	0.21665450378455525
7	0.0363668123595132	0.17369102021623953
8	0.027352836328222774	0.14930228145345295
9	0.02159618961840065	0.13261533044662333
>10	0.1082111732808687	1.3688089435231623
>50	0.01118076988787023	0.5345845411057192
>100	0.009791413137474276	1.2764952229022515
>500	0.0012921740378479256	0.6331086202945522
>1k	0.001216794672261447	1.635290776407142
>5k	2.7039878031549845E-4	1.2550310273956367
>10k+	4.206203249352198E-4	19.125993514965415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	743674	7.590260611120524	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	181225	1.8496612484103478	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143859	1.4682875846878976	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132729	1.35468995911302	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77589	0.791907113273061	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57073	0.5825118853939786	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51290	0.5234880696977058	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48347	0.4934505304284458	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41202	0.42052554976964074	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38398	0.3919067050156465	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33801	0.34498772165825997	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31493	0.32143126884363127	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26476	0.2702255826343626	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25890	0.2642446115124508	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24795	0.25306856479147233	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23827	0.24318873536142005	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23164	0.2364218687166632	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22359	0.22820568825055576	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19961	0.20373065625338085	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16728	0.1707332507292498	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16683	0.17027396113797671	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16643	0.16986570372351176	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12883	0.13148950676380472	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12167	0.12418169904488177	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11688	0.11929281650666379	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11064	0.11292400084101029	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	10671	0.10891287174389197	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10347	0.1056059866867257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013166301616495233	0.0	0.0	1.0206435361624212E-5	0.0
2	0.0030006919963175183	0.0	0.0	1.0206435361624212E-5	0.0
3	0.005205282034428348	0.0	0.0	2.0412870723248423E-5	0.0
4	0.008593818574487586	0.0	0.0	3.061930608487264E-5	0.0
5	0.014186945152657653	0.0	0.0	7.144504753136948E-5	0.0
6	0.024087187453433138	0.0	0.0	7.144504753136948E-5	0.0
7	0.03517137625615703	0.0	0.0	7.144504753136948E-5	0.0
8	0.06983243074423286	0.0	0.0	9.18579182546179E-5	0.0
9	0.19926023756498948	0.0	0.0	1.0206435361624211E-4	0.0
10-11	0.4734152978135774	0.0	0.0	1.0206435361624211E-4	0.0
12-13	0.817244589058293	0.0	0.0	1.0716757129705422E-4	0.0
14-15	1.2137339835513088	0.0	0.0	1.3268365970111476E-4	0.0
16-17	1.6637561315160436	1.0206435361624212E-5	0.0	1.4799331274355107E-4	0.0
18-19	2.1611820685178422	1.0206435361624212E-5	0.0	1.633029657859874E-4	0.0
20-21	2.822084480706775	1.0206435361624212E-5	0.0	1.735094011476116E-4	0.0
22-23	3.5638984092249846	1.0206435361624212E-5	0.0	1.888190541900479E-4	0.0
24-25	4.309299899772805	1.0206435361624212E-5	0.0	2.1433514259410844E-4	0.0
26-27	5.05561486628549	1.0206435361624212E-5	0.0	2.1433514259410844E-4	0.0
28-29	5.865765982767455	1.0206435361624212E-5	0.0	2.3474801331735686E-4	0.0
30-31	6.796924596896836	1.0206435361624212E-5	0.0	2.3474801331735686E-4	0.0
32-33	7.822855066576578	1.0206435361624212E-5	0.0	2.500576663597932E-4	0.0
34-35	8.71003415073272	1.0206435361624212E-5	0.0	2.551608840406053E-4	0.0
36-37	9.339669148191318	1.0206435361624212E-5	0.0	2.755737547638537E-4	0.0
38-39	9.782955048817382	2.0412870723248423E-5	0.0	3.623284553376595E-4	0.0
40-41	10.142492044083635	2.0412870723248423E-5	0.0	3.776381083800958E-4	0.0
42-43	10.426128882783171	2.0412870723248423E-5	0.0	4.388767205498411E-4	0.0
44-45	10.656018632868395	2.0412870723248423E-5	0.0	5.154249857620227E-4	0.0
46-47	10.852910977429488	2.0412870723248423E-5	0.0	5.25631421123647E-4	0.0
48-49	11.037509670597505	2.0412870723248423E-5	0.0	5.511475095277074E-4	0.0
50-51	11.225614274312239	2.0412870723248423E-5	0.0	6.276957747398889E-4	0.0
52-53	11.427242404881126	2.0412870723248423E-5	0.0	6.7872795154801E-4	0.0
54-55	11.632937799941619	2.0412870723248423E-5	0.0	8.21618046610749E-4	0.0
56-57	11.852335334475093	2.0412870723248423E-5	0.0	8.879598764613064E-4	0.0
58-59	12.0866444710719	2.0412870723248423E-5	0.0	9.594049239926758E-4	0.0
60-61	12.330864056404845	2.0412870723248423E-5	0.0	9.798177947159244E-4	0.0
62-63	12.561692798543337	2.0412870723248423E-5	0.0	0.0010563660599281059	0.0
64-65	12.783177549108263	2.0412870723248423E-5	0.0	0.0011380175428210995	0.0
66-67	12.984667892799768	2.0412870723248423E-5	0.0	0.0012247722433949054	0.0
68-69	13.16427563907595	2.0412870723248423E-5	0.0	0.0012758044202030264	0.0
70-71	13.331140650803144	2.0412870723248423E-5	0.0	0.0013268365970111476	0.0
72-73	13.492264542639425	2.0412870723248423E-5	0.0	0.0013625591207768323	0.0
74-75	13.64977535635769	2.0412870723248423E-5	0.0	0.0013829719915000806	0.0
76-77	13.809378489325088	2.0412870723248423E-5	0.0	0.0014340041683082016	0.0
78-79	13.967511895600413	2.0412870723248423E-5	0.0	0.0015309653042436316	0.0
80-81	14.126834351595368	2.0412870723248423E-5	0.0	0.0017095779230720553	0.0
82-83	14.288121546397434	2.0412870723248423E-5	0.0	0.0017504036645185524	0.0
84-85	14.45096522259215	2.0412870723248423E-5	0.0	0.0017963326236458612	0.0
86-87	14.617478112299366	2.0412870723248423E-5	0.0	0.0018116422766882975	0.0
88	14.746564003535509	2.0412870723248423E-5	0.0	0.001837158365092358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGACG	3060	0.0	37.015358	8
TACCCGA	2825	0.0	35.26896	6
GCCGATC	240	0.0	35.248703	7
CTACCCG	2710	0.0	34.86206	5
CCGTTTG	340	0.0	33.183563	1
CGTCAGT	85	1.7550297E-5	33.17949	3
GCATGCC	215	0.0	32.793686	3
AGTTGCC	175	5.456968E-12	32.231506	3
CATGCCG	235	0.0	32.002098	4
GGACCCA	355	0.0	31.781439	1
GGACACG	105	2.0640182E-6	31.34003	1
GATACCG	165	8.731149E-11	31.340029	1
GTTAGCC	105	2.0659954E-6	31.336185	3
ACCCGAC	3385	0.0	31.100777	7
CCGACGC	3695	0.0	31.036028	9
TGCCGAT	215	0.0	30.603058	6
CCTGCTG	1250	0.0	30.0792	8
GTATCCG	110	2.966508E-6	29.915482	1
GCCGCCA	330	0.0	29.911049	4
ATTGCGC	95	3.7520105E-5	29.687822	2
>>END_MODULE