##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248451_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9797740
Sequences flagged as poor quality	0
Sequence length	100
%GC	24
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62182513518424	34.0	31.0	34.0	31.0	34.0
2	32.845345151024624	34.0	31.0	34.0	31.0	34.0
3	32.9428637624595	34.0	33.0	34.0	31.0	34.0
4	36.3169611563483	37.0	37.0	37.0	35.0	37.0
5	36.24004025418107	37.0	37.0	37.0	35.0	37.0
6	36.16807233096612	37.0	37.0	37.0	35.0	37.0
7	36.230038457848444	37.0	37.0	37.0	35.0	37.0
8	35.924815824873896	37.0	35.0	37.0	35.0	37.0
9	37.6884435594331	39.0	38.0	39.0	35.0	39.0
10-11	37.96264414038339	39.0	39.0	39.0	35.0	39.0
12-13	38.08870310908434	39.0	39.0	39.0	36.0	39.0
14-15	39.69216656085995	41.0	40.0	41.0	37.0	41.0
16-17	39.66744626822104	41.0	40.0	41.0	37.0	41.0
18-19	39.45764630414769	41.0	39.0	41.0	36.5	41.0
20-21	39.04047499729529	41.0	38.5	41.0	35.0	41.0
22-23	38.53200778955147	40.0	37.0	41.0	35.0	41.0
24-25	38.10295772290344	40.0	35.0	41.0	35.0	41.0
26-27	37.9281512369179	40.0	35.0	41.0	35.0	41.0
28-29	37.7975205506576	40.0	35.0	41.0	35.0	41.0
30-31	37.73886187018639	40.0	35.0	41.0	34.0	41.0
32-33	37.67848279297063	40.0	35.0	41.0	34.0	41.0
34-35	37.616969933882714	40.0	35.0	41.0	34.0	41.0
36-37	37.55086708771614	40.0	35.0	41.0	34.0	41.0
38-39	37.46193836537813	40.0	35.0	41.0	33.5	41.0
40-41	37.362612449401595	39.0	35.0	41.0	33.0	41.0
42-43	37.24099792401105	39.0	35.0	41.0	33.0	41.0
44-45	37.12781621067715	39.0	35.0	41.0	33.0	41.0
46-47	37.00799010792285	39.0	35.0	41.0	33.0	41.0
48-49	36.89016849804139	38.5	35.0	41.0	33.0	41.0
50-51	36.70293358468381	38.0	35.0	41.0	33.0	41.0
52-53	36.45462923082262	37.0	35.0	40.5	33.0	41.0
54-55	36.29712474509428	37.0	35.0	40.0	33.0	41.0
56-57	36.110445163884734	36.0	35.0	40.0	33.0	41.0
58-59	35.90959996897244	35.5	35.0	40.0	32.0	41.0
60-61	35.668928446764255	35.0	35.0	39.5	31.5	41.0
62-63	35.41410401786534	35.0	35.0	39.0	31.0	41.0
64-65	35.201408590144254	35.0	35.0	39.0	31.0	41.0
66-67	34.99438166352648	35.0	35.0	39.0	31.0	41.0
68-69	34.80609232333171	35.0	35.0	37.5	31.0	40.5
70-71	34.674227525939656	35.0	35.0	37.0	31.0	40.0
72-73	34.52419307922031	35.0	35.0	37.0	31.0	39.5
74-75	34.33180508974519	35.0	35.0	36.0	31.0	39.0
76-77	34.142240251323265	35.0	34.0	36.0	30.5	39.0
78-79	33.95133546103489	35.0	34.0	36.0	30.0	38.5
80-81	33.77643466758661	35.0	34.0	35.0	30.0	37.0
82-83	33.613571905357766	35.0	34.0	35.0	30.0	37.0
84-85	33.448537826070094	35.0	34.0	35.0	29.5	36.5
86-87	33.30787788816605	35.0	34.0	35.0	29.0	36.0
88-89	33.17590719900711	35.0	34.0	35.0	29.0	36.0
90-91	33.06048302975992	35.0	34.0	35.0	29.0	36.0
92-93	32.92334864979067	35.0	34.0	35.0	29.0	36.0
94-95	32.775145390671724	35.0	34.0	35.0	29.0	35.0
96-97	32.654949968053856	35.0	34.0	35.0	27.5	35.0
98-99	32.50733873321806	35.0	33.0	35.0	27.0	35.0
100	31.963340321339412	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17250958591542798
1101	2	0.17814957373554563
1101	3	0.16245559265220066
1101	4	0.052719350462744785
1101	5	0.10196849733264912
1101	6	-0.02383927996129387
1101	7	0.036071556903330304
1101	8	-0.050509550351179655
1101	9	-0.1413481969672148
1101	10-11	9.509236179994218E-4
1101	12-13	0.09441698862750769
1101	14-15	0.14232814413934847
1101	16-17	0.2024790799816003
1101	18-19	0.2380783421556174
1101	20-21	0.27311230886850524
1101	22-23	0.25078066969767576
1101	24-25	0.23857165117200907
1101	26-27	0.2413289986089282
1101	28-29	0.28946992196405574
1101	30-31	0.28908058426173966
1101	32-33	0.31836248884684437
1101	34-35	0.32988485123999567
1101	36-37	0.32988359120066946
1101	38-39	0.3147928857660176
1101	40-41	0.29686720667439914
1101	42-43	0.30075928020090714
1101	44-45	0.27087852312815386
1101	46-47	0.30879209986795075
1101	48-49	0.3274146932360722
1101	50-51	0.309282820491525
1101	52-53	0.2304460920824951
1101	54-55	0.24627837310536194
1101	56-57	0.23854752488958297
1101	58-59	0.20850582763434744
1101	60-61	0.22224091072430951
1101	62-63	0.17293018065009136
1101	64-65	0.17720763170954967
1101	66-67	0.18511581105106245
1101	68-69	0.14753147902229813
1101	70-71	0.10725621781827499
1101	72-73	0.17230488105818154
1101	74-75	0.1833632606350264
1101	76-77	0.1755050932336104
1101	78-79	0.18553422478095172
1101	80-81	0.13593298452402536
1101	82-83	0.12971023089701816
1101	84-85	0.09233618867452975
1101	86-87	0.1650283007889044
1101	88-89	0.2419440108417561
1101	90-91	0.21816726595858427
1101	92-93	0.20831251057992972
1101	94-95	0.21244262741300446
1101	96-97	0.17868345466153812
1101	98-99	0.1747913011648521
1101	100	0.17934736401491946
1102	1	0.13756072527895213
1102	2	0.13171874968924868
1102	3	0.155965120441806
1102	4	0.07227466024779261
1102	5	0.07561822401579832
1102	6	-0.08229775484868895
1102	7	-0.028936576353558507
1102	8	-0.10425741806410826
1102	9	-0.07235740414866143
1102	10-11	0.015702056387951302
1102	12-13	0.11574275345501661
1102	14-15	0.195818507940551
1102	16-17	0.22162316844228513
1102	18-19	0.24786405052751093
1102	20-21	0.19088046334205444
1102	22-23	0.15767618253273952
1102	24-25	0.1354863447872674
1102	26-27	0.13321285882320666
1102	28-29	0.14781069818077697
1102	30-31	0.16329630262659833
1102	32-33	0.15923134363792713
1102	34-35	0.18063050827613125
1102	36-37	0.13792501537480462
1102	38-39	0.17679850543518683
1102	40-41	0.14998541346495387
1102	42-43	0.145784194590874
1102	44-45	0.18047681458942577
1102	46-47	0.19565134623257308
1102	48-49	0.16497372604652583
1102	50-51	0.18352707833275161
1102	52-53	0.19200874141222357
1102	54-55	0.18121089235638976
1102	56-57	0.15118317325449482
1102	58-59	0.18583991247649578
1102	60-61	0.19949799363594423
1102	62-63	0.16963068398070646
1102	64-65	0.15387858199753168
1102	66-67	0.16539184902815407
1102	68-69	0.206053324848952
1102	70-71	0.20517301779043606
1102	72-73	0.14253854304919855
1102	74-75	0.12825312350982188
1102	76-77	0.133297947063312
1102	78-79	0.15641755496039167
1102	80-81	0.18024422235638582
1102	82-83	0.17384726531972916
1102	84-85	0.17056074337227045
1102	86-87	0.1545801064873018
1102	88-89	0.13505276378260334
1102	90-91	0.19497063111975876
1102	92-93	0.2106006305013608
1102	94-95	0.19507221736203917
1102	96-97	0.21816172247916654
1102	98-99	0.23403170436588283
1102	100	0.22520029988251267
1103	1	0.17078414795480512
1103	2	0.17991459949674038
1103	3	0.15689331037876997
1103	4	0.09320881195214525
1103	5	0.1194174599701725
1103	6	0.049874768718275675
1103	7	0.04543414772351895
1103	8	-0.01554102303256144
1103	9	-0.12291082471575265
1103	10-11	0.011934467706353757
1103	12-13	0.1091063516666324
1103	14-15	0.19562897963695747
1103	16-17	0.1953049530534372
1103	18-19	0.1614290467576751
1103	20-21	0.1525632706519673
1103	22-23	0.11428788225047981
1103	24-25	0.08283015051829068
1103	26-27	0.10194938858042235
1103	28-29	0.11425245817181917
1103	30-31	0.09874696649398373
1103	32-33	0.12825290434398084
1103	34-35	0.10448624096682124
1103	36-37	0.11549072508442748
1103	38-39	0.1129648627194797
1103	40-41	0.13958793335749675
1103	42-43	0.15315430414355546
1103	44-45	0.1341165139421605
1103	46-47	0.13823283860214275
1103	48-49	0.14955350584520488
1103	50-51	0.16340021769621416
1103	52-53	0.10227506936471542
1103	54-55	0.1420404996870417
1103	56-57	0.14079486392071772
1103	58-59	0.12027709423627186
1103	60-61	0.11710616656585415
1103	62-63	0.09812405766095367
1103	64-65	0.08739137178663725
1103	66-67	0.07439777813669934
1103	68-69	0.052813705467556815
1103	70-71	0.06945824236828457
1103	72-73	0.10065061375668449
1103	74-75	0.06921965455871515
1103	76-77	0.04547748371875571
1103	78-79	0.060761642511522496
1103	80-81	0.05094837386992168
1103	82-83	0.051189959182359246
1103	84-85	0.023255125244389774
1103	86-87	0.03719101994651197
1103	88-89	0.09243174748841199
1103	90-91	0.04468625151296379
1103	92-93	-0.004468222774576702
1103	94-95	0.03987068234760471
1103	96-97	0.0441707980896382
1103	98-99	0.10522516053723052
1103	100	0.07617515404305308
1104	1	-0.25998465042633256
1104	2	-0.19318181634130127
1104	3	-0.18662074911712523
1104	4	-0.21392205745146953
1104	5	-0.09312745103377296
1104	6	-0.07966216040149021
1104	7	0.005878164407697284
1104	8	-0.15304314526390783
1104	9	-0.027374221387177045
1104	10-11	0.05896190987841976
1104	12-13	0.08552351466776287
1104	14-15	0.12127451079131646
1104	16-17	0.16444269045656057
1104	18-19	0.1724133147140492
1104	20-21	0.19466507122186982
1104	22-23	0.15434984567009735
1104	24-25	0.1387370373310901
1104	26-27	0.1670715488054455
1104	28-29	0.2005303683819335
1104	30-31	0.2029984841761845
1104	32-33	0.20343518680953565
1104	34-35	0.19396195231582425
1104	36-37	0.1708417019991515
1104	38-39	0.165391376157217
1104	40-41	0.13955024941166272
1104	42-43	0.13683078890248623
1104	44-45	0.13690168447779172
1104	46-47	0.16505901867748918
1104	48-49	0.1758679126987559
1104	50-51	0.17926544568122438
1104	52-53	0.15420908173560832
1104	54-55	0.16392216750850253
1104	56-57	0.1674393351065504
1104	58-59	0.20017818678391563
1104	60-61	0.1766125584972471
1104	62-63	0.1366443060423208
1104	64-65	0.12718860118493325
1104	66-67	0.09913086452642972
1104	68-69	0.09047381921915587
1104	70-71	0.12305126607762418
1104	72-73	0.1484866834301144
1104	74-75	0.1437272108494696
1104	76-77	0.1537432019965621
1104	78-79	0.15927639603442856
1104	80-81	0.15492704142983627
1104	82-83	0.14124590005355486
1104	84-85	0.13990193662046835
1104	86-87	0.1544539093472821
1104	88-89	0.11654138540132664
1104	90-91	0.14084968741698844
1104	92-93	0.12081681583855897
1104	94-95	0.11522151013254245
1104	96-97	0.08513929844066581
1104	98-99	0.14052311608279666
1104	100	0.14910861692809263
1105	1	0.0919038440114548
1105	2	0.08934882931607291
1105	3	0.05487600672123705
1105	4	0.027813925842416154
1105	5	0.052081348954921225
1105	6	0.001735954971628928
1105	7	0.01219189209360394
1105	8	-0.10355644053876745
1105	9	-0.10803643319091094
1105	10-11	0.009187657527590432
1105	12-13	0.05033191532191239
1105	14-15	0.10937800182922075
1105	16-17	0.1186637901347396
1105	18-19	0.09657673333533978
1105	20-21	0.08798330544598798
1105	22-23	0.07562542480281564
1105	24-25	0.039611816888523776
1105	26-27	0.04240880557693316
1105	28-29	0.052223570147177156
1105	30-31	0.06324638423380691
1105	32-33	0.06391492443284363
1105	34-35	0.03790125553659607
1105	36-37	0.03191724814167429
1105	38-39	0.03770960401632806
1105	40-41	0.031461632010419294
1105	42-43	0.04603798749666055
1105	44-45	0.034975900763797085
1105	46-47	0.06748449379616517
1105	48-49	0.06494023986999764
1105	50-51	0.06561875728504418
1105	52-53	-0.2715120231748287
1105	54-55	-0.19106296586713967
1105	56-57	-0.13121418920742656
1105	58-59	-0.1404036413583185
1105	60-61	-0.09705382800090234
1105	62-63	-0.15223544707279046
1105	64-65	-0.18096867206870115
1105	66-67	-0.1865195283082528
1105	68-69	-0.14440141732474387
1105	70-71	-0.13199921657928115
1105	72-73	-0.144803631035046
1105	74-75	-0.12625824455276558
1105	76-77	-0.1401325212012523
1105	78-79	-0.12495652962510917
1105	80-81	-0.1509016074145748
1105	82-83	-0.12373855798325195
1105	84-85	-0.13580779756897243
1105	86-87	-0.10981215969845692
1105	88-89	-0.1817040983241398
1105	90-91	-0.14860004196740562
1105	92-93	-0.1456069230335828
1105	94-95	-0.14348998867628637
1105	96-97	-0.1552265615197399
1105	98-99	-0.18790492704956563
1105	100	-0.16320535608057796
1106	1	0.038497721465908796
1106	2	0.04188043780652606
1106	3	0.07678948214402226
1106	4	0.06133295348188028
1106	5	0.053906519303815514
1106	6	0.04214326175240046
1106	7	0.034113410207474715
1106	8	-0.08647020259979854
1106	9	-0.03455938717418405
1106	10-11	0.05593809086330026
1106	12-13	0.09917652048682157
1106	14-15	0.13010624178752295
1106	16-17	0.11289060255619887
1106	18-19	0.1047960778527468
1106	20-21	0.08876607974901418
1106	22-23	0.09235748324304893
1106	24-25	0.10290575450801498
1106	26-27	0.1409856922136541
1106	28-29	0.13654743029270122
1106	30-31	0.1207896395207868
1106	32-33	0.1177860788476579
1106	34-35	0.11057728830488855
1106	36-37	0.13920511430508498
1106	38-39	0.169048454064189
1106	40-41	0.15692293341827224
1106	42-43	0.14362317868143748
1106	44-45	0.15526357782190559
1106	46-47	0.1951966821352542
1106	48-49	0.1950671033196798
1106	50-51	0.19936758236831764
1106	52-53	0.10077506893059507
1106	54-55	0.1484457406317503
1106	56-57	0.1707463055430054
1106	58-59	0.17224686566395064
1106	60-61	0.15923400505275964
1106	62-63	0.11769519369821779
1106	64-65	0.14813190929538678
1106	66-67	0.15008417449774214
1106	68-69	0.10198433202278778
1106	70-71	0.10193842700775946
1106	72-73	0.13981758720075987
1106	74-75	0.11454324901171731
1106	76-77	0.14932986633732526
1106	78-79	0.1728475472762412
1106	80-81	0.1404634022005169
1106	82-83	0.1405207148619425
1106	84-85	0.1492539570302327
1106	86-87	0.12804025867547608
1106	88-89	0.14123902731629556
1106	90-91	0.11805273778873016
1106	92-93	0.15489749250588858
1106	94-95	0.12029896024972686
1106	96-97	0.11335587212428777
1106	98-99	0.11873727636364606
1106	100	0.08906390758329152
1107	1	0.04560898518159462
1107	2	0.0484942430191353
1107	3	0.06670306651800928
1107	4	0.012473512808305998
1107	5	-0.0015367071949228261
1107	6	0.007908545227664376
1107	7	-0.014973511589161603
1107	8	-0.09594586265181704
1107	9	-0.08577311259790577
1107	10-11	-0.0254939718924021
1107	12-13	0.009997011645786813
1107	14-15	0.03226010819789593
1107	16-17	0.03959368538762931
1107	18-19	0.07082588870103734
1107	20-21	0.033246020963837
1107	22-23	0.004330626239408275
1107	24-25	0.035747999177658585
1107	26-27	0.05812857582515818
1107	28-29	0.07578746082062082
1107	30-31	0.06475470783410486
1107	32-33	0.06336770004437398
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2216	16-17	-0.35921729056117613
2216	18-19	-0.36732913760594954
2216	20-21	-0.3162072506850677
2216	22-23	-0.22411906093884681
2216	24-25	-0.17732547808079602
2216	26-27	-0.21812231604896937
2216	28-29	-0.17302080804510922
2216	30-31	-0.20687820028719983
2216	32-33	-0.20721737669923357
2216	34-35	-0.20681270085088244
2216	36-37	-0.22836633715604648
2216	38-39	-0.22462656882063214
2216	40-41	-0.19791325908769153
2216	42-43	-0.18302203336097733
2216	44-45	-0.2267414729267614
2216	46-47	-0.196714740540763
2216	48-49	-0.2128306174884358
2216	50-51	-0.23714721132750327
2216	52-53	-0.18882217518451938
2216	54-55	-0.18271639457981337
2216	56-57	-0.21439028253088566
2216	58-59	-0.21319194150743215
2216	60-61	-0.18685134951282123
2216	62-63	-0.17226215438545012
2216	64-65	-0.15353914797765356
2216	66-67	-0.1585488135326898
2216	68-69	-0.13103121639183257
2216	70-71	-0.14703344371053362
2216	72-73	-0.15229464527463676
2216	74-75	-0.11735116476069862
2216	76-77	-0.14523853288265798
2216	78-79	-0.13425164036096504
2216	80-81	-0.15599987529827075
2216	82-83	-0.13971891125621028
2216	84-85	-0.10886313599005604
2216	86-87	-0.1326487782659811
2216	88-89	-0.1572573949436915
2216	90-91	-0.1799439535102607
2216	92-93	-0.14788459594933556
2216	94-95	-0.20340925253844233
2216	96-97	-0.19643183953465382
2216	98-99	-0.21126715399242357
2216	100	-0.13903272782624043
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	15.0
7	115.0
8	370.0
9	927.0
10	1542.0
11	2447.0
12	3688.0
13	5058.0
14	6663.0
15	8033.0
16	9781.0
17	12657.0
18	15149.0
19	18611.0
20	22760.0
21	27835.0
22	33490.0
23	38761.0
24	45068.0
25	53000.0
26	62325.0
27	74125.0
28	90719.0
29	127339.0
30	127482.0
31	155730.0
32	204722.0
33	334233.0
34	425286.0
35	2309889.0
36	1013811.0
37	1989153.0
38	1869189.0
39	700125.0
40	7641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.167161717182235	22.70183260555804	33.6954245083689	24.43558116889082
2	15.054726692126415	24.234954637762975	39.09505376115811	21.61526490895249
3	12.024643669976992	19.975057485260105	45.54754474225609	22.452754102506816
4	11.789608866266816	18.143493206403306	50.126034543753526	19.940863383576353
5	10.324392567801933	17.952305346391462	52.27841813759786	19.44488394820874
6	9.978092414022449	15.00819174072883	48.87500468252578	26.13871116272295
7	13.110339731407446	16.5924896966035	46.53983469657288	23.757335875416167
8	8.298148348496694	15.963926374857875	49.14878329084054	26.589141985804886
9	10.101309077399481	15.19064600611978	50.22793011449579	24.480114801984946
10-11	12.909597985753859	25.53455692566557	49.78632421923566	11.76952086934491
12-13	11.626909778006231	28.262024951588266	48.3017515595253	11.809313710880197
14-15	12.132924601813123	26.81033202980374	48.452373096982285	12.604370271400853
16-17	14.278563935576043	26.827113486175662	49.16593367315382	9.728388905094471
18-19	14.17815954401706	24.541825227973607	49.084671100004236	12.195344128005095
20-21	11.908008428748563	24.41098043986734	51.27902753499727	12.40198359638683
22-23	12.026609218000186	24.887054224025814	51.92525923727761	11.161077320696396
24-25	12.07012192803713	25.086980824234818	51.28569643167985	11.557200816048207
26-27	13.954523760628767	24.86628688146774	49.600641716819084	11.57854764108441
28-29	12.666669626586243	26.508172159333288	50.94779941972307	9.877358794357393
30-31	12.637200844542148	25.40171990849092	51.16058712132424	10.80049212564269
32-33	12.96322852544314	25.771565702438977	51.14581118698184	10.119394585136044
34-35	12.894565906566141	25.76750889718944	51.35565016949263	9.982275026751788
36-37	13.060701810396635	25.30860518133361	51.170196022875736	10.460496985394022
38-39	13.277161471011809	25.55095579432127	50.84775776949685	10.324124965170077
40-41	13.272440685140019	25.896333484031437	50.87777689832277	9.953448932505776
42-43	13.330014200214968	25.99886055343993	50.92550944661824	9.745615799726854
44-45	13.455822029450687	25.523731876551008	50.84980443301497	10.17064166098334
46-47	13.477696053615695	25.35851679083151	51.07237616720938	10.091410988343421
48-49	13.140942173595821	25.294121459479936	51.22742383655422	10.337512530370024
50-51	13.013363736586184	25.28029317480734	51.21541373042972	10.49092935817677
52-53	12.926521071387073	25.369035413064612	51.304381519559264	10.400061995989047
54-55	12.885889059099478	25.3031818697247	51.45203955186475	10.358889519311077
56-57	12.739051813998747	25.362908448384776	51.365572946111236	10.532466791505248
58-59	12.626094141889268	25.383464132028273	51.55696656849557	10.433475157586898
60-61	12.4476740230898	25.08264346612272	51.73618006040711	10.733502450380366
62-63	12.234078144628416	24.993396851833317	51.85335503980595	10.919169963732319
64-65	12.294369903621348	24.915478679210363	51.878502241056566	10.911649176111716
66-67	12.084858578053892	24.762185343787838	52.30291663846629	10.85003943969198
68-69	11.980084328107152	24.708843854984895	52.54791454407447	10.763157272833485
70-71	11.839296209536501	24.665582391355333	52.74189543336008	10.753225965748095
72-73	11.72955828946872	24.40664425931061	53.06226975767932	10.801527693541356
74-75	11.72574495171055	24.154657721192795	53.29928659355902	10.820310733537635
76-77	11.910992586543493	24.13081369384083	53.35431728573069	10.603876433884983
78-79	11.959404871135765	24.38155084800652	53.31465757193904	10.344386708918679
80-81	11.869567436519077	24.321868061758327	53.34250861298967	10.466055888732933
82-83	11.926658744387684	24.196912271095673	53.330957976653416	10.545471007863227
84-85	11.990480989131106	24.1317368142325	53.273561011621005	10.604221185015387
86-87	12.101513443273564	24.331373286575307	53.056647069601645	10.51046620054948
88-89	12.16679188725727	24.711179868877426	52.80605129588638	10.315976947978921
90-91	12.186545110393608	24.906276896142305	52.689221251035015	10.217956742429067
92-93	12.075708527893932	24.988486109123514	52.59014570175634	10.345659661226216
94-95	12.067554944738063	25.27728960353719	52.43002572190839	10.225129729816356
96-97	11.86208121398663	25.76762561124328	52.51418972419765	9.85610345057245
98-99	11.703139109255439	26.31636027889107	52.33945857103463	9.641042040818846
100	11.699211287865078	26.700675532979606	52.100505294708285	9.499607884447023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245676.0
1	555717.0
2	869846.5
3	623613.5
4	233341.5
5	65964.0
6	29803.5
7	18585.5
8	16174.0
9	18262.5
10	23767.0
11	33118.0
12	46989.0
13	64781.0
14	86611.5
15	110905.5
16	136254.0
17	162241.0
18	184565.0
19	199529.0
20	208700.0
21	218063.0
22	231640.5
23	247707.5
24	261693.0
25	269642.0
26	273585.0
27	275584.5
28	275006.5
29	269640.0
30	257894.5
31	239349.5
32	215792.5
33	192253.5
34	170677.0
35	150850.5
36	132586.0
37	116804.5
38	103280.5
39	90789.5
40	79638.0
41	69909.0
42	62281.0
43	57875.0
44	57662.5
45	61684.0
46	68853.0
47	78145.5
48	88728.0
49	102327.5
50	120382.0
51	142477.5
52	164269.5
53	178112.0
54	176974.5
55	156911.5
56	122648.5
57	84698.0
58	52007.0
59	29521.5
60	16283.0
61	8715.0
62	4635.0
63	2584.5
64	1561.0
65	1026.0
66	732.0
67	568.5
68	471.5
69	378.5
70	300.0
71	218.0
72	150.0
73	120.0
74	94.0
75	80.5
76	63.5
77	40.0
78	25.0
79	21.0
80	17.5
81	16.0
82	16.0
83	12.0
84	8.5
85	9.5
86	9.5
87	6.5
88	4.5
89	4.0
90	5.0
91	4.0
92	2.0
93	2.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0077568908748344
2	5.409410741660833E-4
3	1.1227078897786632E-4
4	0.004347941464051914
5	0.013656210513853195
6	0.006909756739819591
7	0.0
8	0.0
9	0.0
10-11	0.011451620475742365
12-13	0.005388997870937584
14-15	0.0011278111074594754
16-17	1.837158365092358E-4
18-19	5.103217680812106E-5
20-21	8.318244819723732E-4
22-23	7.654826521218157E-5
24-25	3.521220199760353E-4
26-27	0.0027761504183617856
28-29	0.0018014358413266734
30-31	0.002469957357513059
32-33	0.002316860827088696
34-35	0.002694498935468792
36-37	0.001102295019055415
38-39	0.0013268365970111476
40-41	5.613539448893316E-5
42-43	1.020643536162421E-5
44-45	7.552762167601916E-4
46-47	1.2247722433949052E-4
48-49	4.5418637359227737E-4
50-51	0.03239522583779525
52-53	0.036314497016658946
54-55	7.450697813985675E-4
56-57	2.1433514259410844E-4
58-59	5.154249857620227E-4
60-61	0.0039141679611828855
62-63	0.013028514739113305
64-65	0.01725908219650654
66-67	0.018075597025436478
68-69	0.018744118541622865
70-71	0.01676407008146777
72-73	0.014324732030039582
74-75	0.015319859477797942
76-77	0.013931784268617048
78-79	0.014712576573781302
80-81	0.015207588688820077
82-83	0.013436772153578273
84-85	0.01371744912602294
86-87	0.012298754610757176
88-89	0.010497318769430502
90-91	0.008124322547852872
92-93	0.008961250247506058
94-95	0.01054324772855781
96-97	0.006098345128570467
98-99	0.008864289111570627
100	0.00901228242431418
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9797740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.5582811560815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.94207696128011	61.19506608462966
2	6.50070345505922	8.653512965482841
3	0.8249854764625645	1.6472884587623768
4	0.22401591535614265	0.596404571108444
5	0.1184741388112412	0.394271752036161
6	0.0705425853580403	0.2817115937842395
7	0.04808256699131679	0.22402051087599312
8	0.03589549989243946	0.19113142192632637
9	0.03091051768262639	0.18516158339402486
>10	0.17136770980924684	2.126696230338123
>50	0.01708078464430681	0.7817977002816395
>100	0.01019578103705029	1.313333365108136
>500	0.0015448524428173154	0.712459035630837
>1k	0.0022240478399143584	3.3971168861735626
>5k	6.641253277816706E-4	3.208472539515314
>10k+	0.001235582005175201	15.091555300952281
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41519	0.4237609897792757	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32726	0.33401580364451394	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32108	0.32770822659103016	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31631	0.3228397569235354	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29086	0.29686437892820183	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28838	0.294333182958519	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27277	0.2784009373590236	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26857	0.27411423450714145	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26661	0.2721137731762631	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26612	0.2716136578435435	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26047	0.2658470218642258	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25860	0.2639384184516021	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25604	0.2613255709990263	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25245	0.2576614607042032	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24955	0.25470159444933216	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24809	0.25321145488653507	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24497	0.2500270470537083	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23791	0.2428213036884016	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23737	0.2422701561788739	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23406	0.2388918260741763	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22678	0.23146154113091386	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22394	0.2285629134882126	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21264	0.2170296415295772	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20652	0.21078330308826324	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20422	0.2084358229550896	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20378	0.2079867397991782	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19889	0.20299579290734396	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19888	0.2029855864719823	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19691	0.20097491870574233	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19535	0.19938271478932898	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19197	0.19593293963709998	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19124	0.1951878698557014	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18905	0.19295266051150572	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18655	0.19040105167109966	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18273	0.18650219336295923	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17779	0.18146021429431686	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17745	0.18111319549202165	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17517	0.1787861282295713	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17422	0.177816516870217	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16923	0.17272350562476654	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16828	0.17175389426541224	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16406	0.16744677854280682	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16331	0.16668129589068498	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16179	0.1651299177157181	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16173	0.16506867910354836	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16058	0.16389493903696156	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16034	0.16364998458828262	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15818	0.1614453945501718	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15672	0.15995525498737465	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15593	0.15914894659380632	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15229	0.1554338041221751	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15224	0.15538277194536698	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14916	0.15223918985398674	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14714	0.15017748991093866	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14468	0.1476667068119791	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14354	0.14650317318075393	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14037	0.14326773317111904	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13589	0.1386952501291114	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13338	0.13613343485334373	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13308	0.135827241792495	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13215	0.13487804330386396	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13198	0.13470453390271633	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13096	0.13366347749583066	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13003	0.13271427900719962	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11853	0.12097687834133176	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11421	0.11656769826511011	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11272	0.1150469393962281	No Hit
GCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11207	0.11438352109772254	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10997	0.11224016967178146	No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10714	0.10935174846444179	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10703	0.10923947767546394	No Hit
GATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10673	0.1089332846146152	No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10590	0.1080861504796004	No Hit
GTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10542	0.10759624158224244	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10524	0.10741252574573319	No Hit
GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10439	0.10654497873999515	No Hit
GCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10295	0.10507525204792126	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10180	0.10390151198133447	No Hit
AATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10163	0.10372800258018687	No Hit
CATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10131	0.10340139664861488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010920885836937905	0.0	0.0	0.0	0.0
2	0.002418925180704938	0.0	0.0	1.0206435361624212E-5	0.0
3	0.004154019192181054	0.0	0.0	2.0412870723248423E-5	0.0
4	0.006991408222712585	0.0	0.0	2.0412870723248423E-5	0.0
5	0.012543709059436156	0.0	0.0	2.0412870723248423E-5	0.0
6	0.022290854829787276	0.0	0.0	2.0412870723248423E-5	0.0
7	0.032976992653407824	0.0	0.0	3.061930608487264E-5	0.0
8	0.07722188994604878	0.0	0.0	3.061930608487264E-5	0.0
9	0.25016993714877106	0.0	0.0	3.061930608487264E-5	0.0
10-11	0.5989952785030017	0.0	0.0	3.061930608487264E-5	0.0
12-13	0.9774192824059426	0.0	0.0	3.061930608487264E-5	0.0
14-15	1.3853858134631047	0.0	0.0	3.572252376568474E-5	0.0
16-17	1.8224917174777042	0.0	0.0	7.654826521218159E-5	0.0
18-19	2.2960448021686632	0.0	0.0	1.2758044202030265E-4	0.0
20-21	2.9268025075170394	0.0	0.0	1.4289009506273897E-4	0.0
22-23	3.6488873964812294	0.0	0.0	1.4289009506273897E-4	0.0
24-25	4.413339198631521	0.0	0.0	1.5309653042436318E-4	0.0
26-27	5.200719757821702	0.0	0.0	1.5309653042436318E-4	0.0
28-29	6.067940157628188	0.0	0.0	1.5309653042436318E-4	0.0
30-31	7.052243680685546	0.0	0.0	1.5309653042436318E-4	0.0
32-33	8.15240555475038	0.0	0.0	1.5309653042436318E-4	0.0
34-35	9.140255814095903	0.0	0.0	1.5309653042436318E-4	0.0
36-37	9.80789957684119	0.0	0.0	1.786126188284237E-4	0.0
38-39	10.263075974663543	0.0	0.0	2.1433514259410844E-4	0.0
40-41	10.655462382141188	0.0	0.0	2.449544486789811E-4	0.0
42-43	10.970397254877145	0.0	0.0	3.010898431679142E-4	0.0
44-45	11.21983743189756	0.0	0.0	3.36812366933599E-4	0.0
46-47	11.429615401102703	0.0	0.0	3.470188022952232E-4	0.0
48-49	11.62120039927575	0.0	0.0	3.7253489069928375E-4	0.0
50-51	11.81147387050483	0.0	0.0	4.33773502869029E-4	0.0
52-53	12.017021272252581	0.0	0.0	5.766635979317679E-4	0.0
54-55	12.21231630967958	0.0	0.0	6.634182985055738E-4	0.0
56-57	12.407958365908872	0.0	0.0	6.889343869096343E-4	0.0
58-59	12.598002192342316	0.0	0.0	7.042440399520707E-4	0.0
60-61	12.78704578811032	0.0	0.0	7.603794344410037E-4	0.0
62-63	12.974655379710015	0.0	0.0	8.165148289299369E-4	0.0
64-65	13.166061765264235	0.0	0.0	9.236824002269912E-4	0.0
66-67	13.3414185312123	0.0	0.0	0.0010257467538432332	0.0
68-69	13.499225331556053	0.0	0.0	0.0010716757129705422	0.0
70-71	13.65027547169041	0.0	0.0	0.0011329143251402876	0.0
72-73	13.803249524890434	0.0	0.0	0.00116353363122516	0.0
74-75	13.956039862253949	0.0	0.0	0.0012553915494797779	0.0
76-77	14.110861280254426	0.0	0.0	0.0013727655561384564	0.0
78-79	14.2606305127509	0.0	0.0	0.0015564813926476923	0.0
80-81	14.410522222471712	0.0	0.0	0.0017657133175609887	0.0
82-83	14.563822881603308	0.0	0.0	0.0018116422766882975	0.0
84-85	14.718945389446954	0.0	0.0	0.001832055147411546	0.0
86-87	14.878084129605398	0.0	0.0	0.001837158365092358	0.0
88	15.002551608840406	0.0	0.0	0.0018473648004539822	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAGGC	690	0.0	64.03301	1
GCCTTTT	2790	0.0	58.2904	1
GCTTTCT	2320	0.0	57.3355	1
TGCTCTA	2830	0.0	55.973717	6
TGCCCTA	3380	0.0	55.070522	6
GCCCTAC	7360	0.0	52.494877	7
GCTTTTC	2505	0.0	50.849506	1
GCATATC	845	0.0	50.064114	6
GGCATAT	860	0.0	48.64408	5
GATTTCT	2535	0.0	48.578995	1
AGGCATA	900	0.0	46.999355	4
GACTTTT	2600	0.0	45.55671	1
CCCCTAC	20625	0.0	45.418865	7
CTAGCTT	465	0.0	44.475914	1
GCCCCTA	2455	0.0	44.228397	6
GCTGCGT	385	0.0	43.952164	5
CGCCCTA	2935	0.0	43.40121	6
TTGCGTC	425	0.0	43.133667	6
CTGCGTG	365	0.0	42.49734	6
TTAGGCA	1050	0.0	42.07583	2
>>END_MODULE