FastQCFastQC Report
Thu 26 May 2016
SRR1248450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11828558
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3449832.9165262578921287No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA839010.7093087762684175No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA707710.5983062347921022No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA623480.5270972167528789No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA375530.3174774135613149No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259390.219291311755837No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242690.2051729382398091No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239150.2021801812190463No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193060.16321516113798487No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171040.1445991979749349No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165830.1401946036025693No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129450.10943853003891092No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN127100.10745181280761357No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121740.10292040669708005No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118300.10001219083509588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGA31450.037.8090829
CCTGCTG22050.035.8092428
GCCTGCT8750.034.3767
TTGCTGA39050.033.700269
CAGTCCG1405.11136E-1033.5791241
CTTGCTG23600.033.258218
TAGCCGA3600.030.0289385
ACTTGCC7900.029.7489933
ATGCGCC3500.029.5453
CCCGACG49250.029.392668
TGCCAAA18850.027.923386
TTGCGCC4050.027.8538723
CTAGCCG3800.027.2118074
GCTTGCT9400.026.9991027
TGTGCCG4200.026.858414
AGCCGAC3350.026.6544746
TGAACCG57800.026.0624812-13
TGCGCCT3450.025.8852794
TCGCGAC4000.025.8478266
GCTGAAC65750.025.6980610-11
Produced by FastQC (version 0.11.5)