##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248450_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11828558
Sequences flagged as poor quality	0
Sequence length	100
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29158575373262	31.0	31.0	34.0	25.0	34.0
2	30.635617883430932	33.0	31.0	34.0	26.0	34.0
3	30.837655274632798	33.0	31.0	34.0	26.0	34.0
4	34.157916374929215	37.0	35.0	37.0	30.0	37.0
5	34.04275136495928	37.0	35.0	37.0	28.0	37.0
6	33.84259281647011	37.0	35.0	37.0	28.0	37.0
7	34.005825731251434	37.0	35.0	37.0	28.0	37.0
8	34.041382051810544	37.0	35.0	37.0	28.0	37.0
9	35.48328883368539	39.0	35.0	39.0	28.0	39.0
10-11	36.002104905771276	39.0	37.0	39.0	31.0	39.0
12-13	36.09369658583912	39.0	37.0	39.0	31.0	39.0
14-15	37.32145418739968	40.0	38.0	41.0	31.0	41.0
16-17	37.22621755754167	40.0	38.0	41.0	30.5	41.0
18-19	37.006745454517784	40.0	36.5	41.0	30.0	41.0
20-21	36.814802954003355	40.0	36.0	41.0	30.0	41.0
22-23	36.631792860972574	40.0	35.0	41.0	29.5	41.0
24-25	36.499654564825235	40.0	35.0	41.0	28.0	41.0
26-27	36.36731827328403	40.0	35.0	41.0	27.0	41.0
28-29	36.24142710379405	39.0	35.0	41.0	26.0	41.0
30-31	36.15195356018883	39.0	35.0	41.0	25.0	41.0
32-33	36.07412378584101	39.0	35.0	41.0	25.0	41.0
34-35	35.98305761361613	39.0	35.0	41.0	25.0	41.0
36-37	35.86204421536421	39.0	35.0	41.0	25.0	41.0
38-39	35.74298181570399	39.0	35.0	41.0	25.0	41.0
40-41	35.619421192338066	39.0	35.0	41.0	25.0	41.0
42-43	35.48680676038448	39.0	35.0	41.0	25.0	41.0
44-45	35.31424430602614	38.5	34.0	40.5	25.0	41.0
46-47	35.143752306916866	38.0	34.0	40.0	25.0	41.0
48-49	34.93417502792818	38.0	33.0	40.0	25.0	41.0
50-51	34.69047190705748	37.5	33.0	40.0	25.0	41.0
52-53	34.41784023885245	37.0	33.0	40.0	25.0	41.0
54-55	34.178552153187226	36.5	33.0	40.0	25.0	41.0
56-57	33.923123131323365	36.0	32.0	40.0	25.0	41.0
58-59	33.98221156796966	36.0	32.0	39.0	25.0	41.0
60-61	33.79117247427793	35.0	32.0	39.0	25.0	41.0
62-63	33.54148607970642	35.0	32.0	39.0	25.0	41.0
64-65	33.298022590750286	35.0	32.0	39.0	25.0	41.0
66-67	33.05559232156616	35.0	31.0	38.0	24.5	40.0
68-69	32.80918058650936	35.0	31.0	37.0	24.0	40.0
70-71	32.567359182750764	35.0	31.0	37.0	24.0	39.5
72-73	32.48208361492584	35.0	31.0	36.0	24.0	39.0
74-75	32.47521629432768	35.0	31.5	36.0	24.0	39.0
76-77	32.31402272364899	35.0	31.5	36.0	24.0	39.0
78-79	32.10607831487152	35.0	31.0	35.0	24.0	37.5
80-81	31.905587350546025	35.0	31.0	35.0	24.0	37.0
82-83	31.70938486331132	35.0	31.0	35.0	23.5	36.5
84-85	31.526229232675696	35.0	31.0	35.0	23.0	36.0
86-87	31.34258634907146	35.0	31.0	35.0	23.0	36.0
88-89	31.166185218857613	35.0	30.5	35.0	20.5	36.0
90-91	30.97386832782153	35.0	30.0	35.0	20.0	36.0
92-93	30.787792730102858	35.0	30.0	35.0	20.0	35.0
94-95	30.57688714042743	34.5	30.0	35.0	19.0	35.0
96-97	30.35410816770734	34.0	29.0	35.0	18.0	35.0
98-99	30.131497262810903	34.0	29.0	35.0	16.0	35.0
100	29.274775336097605	34.0	27.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20995515290507072
1101	2	0.24071234956575793
1101	3	0.2539014462731046
1101	4	0.1606631849100495
1101	5	0.032072379855470956
1101	6	0.08546441079010947
1101	7	0.1061985239882759
1101	8	0.11216897286593763
1101	9	0.2154898341157505
1101	10-11	0.30405151864183466
1101	12-13	0.28455545165873986
1101	14-15	0.41892397408645365
1101	16-17	0.3977141956775938
1101	18-19	0.4354962023126774
1101	20-21	0.44487511227396226
1101	22-23	0.4378589574146261
1101	24-25	0.47199182681897156
1101	26-27	0.46996644291363054
1101	28-29	0.4701646962946313
1101	30-31	0.47925192416965245
1101	32-33	0.49024926048392814
1101	34-35	0.4975522507692034
1101	36-37	0.4868277397847294
1101	38-39	0.43788654251797254
1101	40-41	0.4911163098645659
1101	42-43	0.48109626847307396
1101	44-45	0.4343920748859844
1101	46-47	0.47367278645497635
1101	48-49	0.4680671129662528
1101	50-51	0.495654410645777
1101	52-53	0.515432798988229
1101	54-55	0.47602371817207256
1101	56-57	0.4495777139209238
1101	58-59	0.4244813845053841
1101	60-61	0.38055732279048016
1101	62-63	0.43580744685415596
1101	64-65	0.4159741067897684
1101	66-67	0.3444617361205573
1101	68-69	0.30257119356998174
1101	70-71	0.364172315038374
1101	72-73	0.3074789636300821
1101	74-75	0.3302558905844606
1101	76-77	0.3660789585280142
1101	78-79	0.35713679588022273
1101	80-81	0.31322845296354984
1101	82-83	0.29893387850773223
1101	84-85	0.25998962073587606
1101	86-87	0.3034111694663828
1101	88-89	0.3271958690571033
1101	90-91	0.2873175915814805
1101	92-93	0.2574321456283073
1101	94-95	0.2750096699062823
1101	96-97	0.21522775535805394
1101	98-99	0.3084506949768624
1101	100	0.40622473807592385
1102	1	0.06523961136789325
1102	2	0.07386831082911982
1102	3	0.08299745905773648
1102	4	0.06546836125311728
1102	5	0.012044949544623762
1102	6	-0.14085306503522332
1102	7	-0.0066620679467988
1102	8	0.07509965744302605
1102	9	0.11316216334024176
1102	10-11	0.15201760280091747
1102	12-13	0.1668819192794757
1102	14-15	0.27364257901658107
1102	16-17	0.27807441760998586
1102	18-19	0.25356194427153156
1102	20-21	0.26857061631783097
1102	22-23	0.22996594299775808
1102	24-25	0.17124423264537114
1102	26-27	0.22101432914777774
1102	28-29	0.2383293805424529
1102	30-31	0.22576822255724238
1102	32-33	0.269493296811568
1102	34-35	0.2822069374840197
1102	36-37	0.2798536803319891
1102	38-39	0.2569145026753503
1102	40-41	0.2894535158101945
1102	42-43	0.2892625118333072
1102	44-45	0.21738460350607625
1102	46-47	0.2666831965527763
1102	48-49	0.3062832591594926
1102	50-51	0.31948391565928347
1102	52-53	0.26680891681092334
1102	54-55	0.21390154506331527
1102	56-57	0.2496429188871261
1102	58-59	0.2728858561062779
1102	60-61	0.2695757422046654
1102	62-63	0.2252980517075187
1102	64-65	0.29377684504778756
1102	66-67	0.3188295821081226
1102	68-69	0.2826824069589904
1102	70-71	0.29128080557621416
1102	72-73	0.3269595697594099
1102	74-75	0.2709063397854621
1102	76-77	0.25358325687372485
1102	78-79	0.21584103189738357
1102	80-81	0.2016362791954549
1102	82-83	0.22466535009276356
1102	84-85	0.17745354036244265
1102	86-87	0.17173418294975917
1102	88-89	0.17478851905335446
1102	90-91	0.1619683137732224
1102	92-93	0.12742930605720204
1102	94-95	0.15422633300213562
1102	96-97	0.2396744603432026
1102	98-99	0.2451638684969062
1102	100	0.22538315086264404
1103	1	0.04356688036719447
1103	2	0.07172663416097791
1103	3	0.11918527876267859
1103	4	0.0750629742143687
1103	5	0.005599991724473341
1103	6	-0.024513717124776235
1103	7	0.028821716304499034
1103	8	0.027938765919138575
1103	9	0.001812600232767636
1103	10-11	0.1306592712875556
1103	12-13	0.14224386752025708
1103	14-15	0.22358151407811278
1103	16-17	0.2716048610009594
1103	18-19	0.2599547157590081
1103	20-21	0.2422677946660201
1103	22-23	0.23096271363970544
1103	24-25	0.27691895993400095
1103	26-27	0.20003058547008123
1103	28-29	0.19756366904179856
1103	30-31	0.19530115529723702
1103	32-33	0.2566737045712415
1103	34-35	0.25439047007881044
1103	36-37	0.23001671357429387
1103	38-39	0.19889343996759834
1103	40-41	0.2643219771991525
1103	42-43	0.23761272328355432
1103	44-45	0.20724699722161688
1103	46-47	0.2022846400713263
1103	48-49	0.2351185914522702
1103	50-51	0.2746557181423199
1103	52-53	0.23406410522270704
1103	54-55	0.18608345387924174
1103	56-57	0.2419003112969662
1103	58-59	0.23582051958583605
1103	60-61	0.2113083284494479
1103	62-63	0.23627642063541998
1103	64-65	0.25298931062701513
1103	66-67	0.23106785316515044
1103	68-69	0.22216507852521516
1103	70-71	0.238154772452134
1103	72-73	0.20498764184885232
1103	74-75	0.1735874574996643
1103	76-77	0.20838501726707648
1103	78-79	0.19863575903240616
1103	80-81	0.25070718548613513
1103	82-83	0.23999020354359146
1103	84-85	0.2666267380430334
1103	86-87	0.25195019479524916
1103	88-89	0.28152889609731346
1103	90-91	0.298829559239163
1103	92-93	0.26435049359138674
1103	94-95	0.21391075888402256
1103	96-97	0.31637367605383204
1103	98-99	0.28793419136258436
1103	100	0.2675741520053805
1104	1	0.0631593603852032
1104	2	0.1006405708940612
1104	3	0.16696915433024273
1104	4	0.11917560508231873
1104	5	0.0726064460004281
1104	6	-0.021508069633313198
1104	7	0.01928139584219224
1104	8	0.11711012319333491
1104	9	0.08585843684952721
1104	10-11	0.14072254758310265
1104	12-13	0.14828771531941953
1104	14-15	0.20834909447512473
1104	16-17	0.24622234195709325
1104	18-19	0.20970752074231314
1104	20-21	0.24442594318941957
1104	22-23	0.22768618356352732
1104	24-25	0.2482445256212742
1104	26-27	0.2183423889148628
1104	28-29	0.21986333319429718
1104	30-31	0.23816749174513774
1104	32-33	0.26700218781638796
1104	34-35	0.24944384919247398
1104	36-37	0.2718795883538334
1104	38-39	0.27348662357674414
1104	40-41	0.2705533554872659
1104	42-43	0.2836872007498741
1104	44-45	0.25757843554950455
1104	46-47	0.24732296233482032
1104	48-49	0.24835032622655717
1104	50-51	0.22745296194133147
1104	52-53	0.18292714886411687
1104	54-55	0.22073311240301052
1104	56-57	0.23566794798126267
1104	58-59	0.18316741815613824
1104	60-61	0.18957993338771928
1104	62-63	0.20527577333157865
1104	64-65	0.19815775034332717
1104	66-67	0.1776769660774562
1104	68-69	0.1502541250185132
1104	70-71	0.16722823674722065
1104	72-73	0.16132967156853795
1104	74-75	0.1823513416979381
1104	76-77	0.18093333052460991
1104	78-79	0.18968021661055445
1104	80-81	0.18784966738039088
1104	82-83	0.1847440873666244
1104	84-85	0.13821877714394049
1104	86-87	0.1885177958309363
1104	88-89	0.15818421149376505
1104	90-91	0.20195195595198356
1104	92-93	0.17415841321313508
1104	94-95	0.15712166736088662
1104	96-97	0.1985220681135722
1104	98-99	0.2208055467605341
1104	100	0.21721184947995908
1105	1	0.10318699203407178
1105	2	0.14242467206840814
1105	3	0.07160338923468501
1105	4	0.09596262883490425
1105	5	0.03863962169166513
1105	6	0.04760196100947667
1105	7	0.02615069460054542
1105	8	0.08847293028545522
1105	9	0.0582595562855559
1105	10-11	0.08507869473934448
1105	12-13	0.06631271664786453
1105	14-15	0.13538369603029565
1105	16-17	0.1336284652013262
1105	18-19	0.15236941386450553
1105	20-21	0.16446065987645397
1105	22-23	0.14916744363756607
1105	24-25	0.1551734272632288
1105	26-27	0.1796276591204986
1105	28-29	0.1931660680190319
1105	30-31	0.14761587724206038
1105	32-33	0.1656992050153221
1105	34-35	0.22052236912800538
1105	36-37	0.19055123225981418
1105	38-39	0.16032293262610153
1105	40-41	0.16005855413402514
1105	42-43	0.15928105979303808
1105	44-45	0.17560193130734092
1105	46-47	0.21737632152854047
1105	48-49	0.1841273335064315
1105	50-51	0.17365316740743708
1105	52-53	0.19301518046338373
1105	54-55	0.19294638976454337
1105	56-57	0.15336398213114677
1105	58-59	0.14356860844254982
1105	60-61	0.13383343605346454
1105	62-63	0.1936455060485187
1105	64-65	0.18181389572831108
1105	66-67	0.2153022384086043
1105	68-69	0.17919787867173653
1105	70-71	0.1893799628396522
1105	72-73	0.19673059616602018
1105	74-75	0.17463192692209617
1105	76-77	0.22019692936974877
1105	78-79	0.20447798305227138
1105	80-81	0.20127129800192733
1105	82-83	0.18454601626826417
1105	84-85	0.19521547374489145
1105	86-87	0.12905448747236292
1105	88-89	0.14259779174067688
1105	90-91	0.13383050954144338
1105	92-93	0.15007623562665628
1105	94-95	0.1115485763358599
1105	96-97	0.12994744494904253
1105	98-99	0.11470094502824324
1105	100	0.14582371337637667
1106	1	0.06044503767088116
1106	2	0.07196017721366843
1106	3	0.09039270608712968
1106	4	0.1092999252066349
1106	5	0.06561905067970031
1106	6	0.047656222864311815
1106	7	0.014827023054486688
1106	8	0.05272222713876573
1106	9	0.08385334317776483
1106	10-11	0.12141386244108077
1106	12-13	0.07854542057712166
1106	14-15	0.16003216449153257
1106	16-17	0.1374206498220687
1106	18-19	0.16199859386671278
1106	20-21	0.10384168177182573
1106	22-23	0.08798640803041735
1106	24-25	0.141861464238211
1106	26-27	0.10666406723653665
1106	28-29	0.1348408865051809
1106	30-31	0.14340987282084683
1106	32-33	0.11568344483097803
1106	34-35	0.07625318183514196
1106	36-37	0.10285429266186696
1106	38-39	0.118456191046306
1106	40-41	0.07794824454882132
1106	42-43	0.14660138533071887
1106	44-45	0.12065557445997399
1106	46-47	0.16209331543851135
1106	48-49	0.12826161863785046
1106	50-51	0.12233662765833486
1106	52-53	0.12715554392584494
1106	54-55	0.14715912466235181
1106	56-57	0.04814614879280299
1106	58-59	0.09748624747496848
1106	60-61	0.08045168509279677
1106	62-63	0.06577046382626861
1106	64-65	0.05418921887479655
1106	66-67	0.049142907543391345
1106	68-69	0.08887948114387001
1106	70-71	0.1498044259900837
1106	72-73	0.09559929083815888
1106	74-75	0.13699953301279777
1106	76-77	0.14888485430946474
1106	78-79	0.14869558395925253
1106	80-81	0.1046214449855043
1106	82-83	0.12218969731223339
1106	84-85	0.11065899125081913
1106	86-87	0.09712372693686788
1106	88-89	0.08347774512062855
1106	90-91	0.07510722660725833
1106	92-93	0.06924776830249257
1106	94-95	0.10087069610991506
1106	96-97	0.11981987524471194
1106	98-99	0.14647117742616445
1106	100	0.07135671695816015
1107	1	0.073358371541552
1107	2	0.06937422024959261
1107	3	0.12491248271511068
1107	4	0.08824713059027545
1107	5	0.11410552320711531
1107	6	0.08504026923252894
1107	7	0.020347344237976017
1107	8	0.05741934921503855
1107	9	0.09709846292984992
1107	10-11	0.12855393551097194
1107	12-13	0.1304429739670212
1107	14-15	0.18360051583168513
1107	16-17	0.16748139202154988
1107	18-19	0.191456495475407
1107	20-21	0.17849767045886722
1107	22-23	0.149889652586765
1107	24-25	0.1653419318181122
1107	26-27	0.18552456679203289
1107	28-29	0.16934081873649376
1107	30-31	0.19716379207665824
1107	32-33	0.18257127201039935
1107	34-35	0.17951803370441155
1107	36-37	0.17547071445900286
1107	38-39	0.17612536114451416
1107	40-41	0.1650354019009015
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2216	48-49	-0.1959258236840924
2216	50-51	-0.22898313101436685
2216	52-53	-0.19428915755025145
2216	54-55	-0.20152731777821487
2216	56-57	-0.20497183643853845
2216	58-59	-0.2534596533965825
2216	60-61	-0.29678266075522686
2216	62-63	-0.3312366131461033
2216	64-65	-0.3009141818003229
2216	66-67	-0.28115072479914005
2216	68-69	-0.32175463853683794
2216	70-71	-0.2977308625812327
2216	72-73	-0.3146521893406202
2216	74-75	-0.2871096058104854
2216	76-77	-0.26635727027667855
2216	78-79	-0.2756399917890775
2216	80-81	-0.3138739338370371
2216	82-83	-0.29138097689128983
2216	84-85	-0.2848630731575632
2216	86-87	-0.25464907890897237
2216	88-89	-0.19575867444130068
2216	90-91	-0.18184427914629708
2216	92-93	-0.24267587480918706
2216	94-95	-0.17293414522632133
2216	96-97	-0.17298379057613644
2216	98-99	-0.14480649456036332
2216	100	-0.08286007858381694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13310.0
1	654.0
2	379.0
3	375.0
4	300.0
5	800.0
6	3959.0
7	8910.0
8	13745.0
9	19914.0
10	28657.0
11	36641.0
12	41739.0
13	45938.0
14	51894.0
15	60334.0
16	67566.0
17	73973.0
18	78809.0
19	81632.0
20	85139.0
21	88984.0
22	94793.0
23	105332.0
24	156197.0
25	164833.0
26	127806.0
27	215287.0
28	152800.0
29	238454.0
30	223563.0
31	274988.0
32	356847.0
33	579538.0
34	706388.0
35	1594105.0
36	1675176.0
37	2010870.0
38	1825992.0
39	517313.0
40	4624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.914163841627088	27.893330772889847	28.5172525967249	27.675252788758165
2	13.548753607188257	28.912046199101976	31.5899905896676	25.949209604042167
3	12.438547274372075	30.343818617994696	29.9610161950638	27.256617912569432
4	11.092781909679283	29.84393595091262	30.469372327714606	28.59390981169349
5	10.94680374715722	29.70167223151555	31.06741728560954	28.284106735717696
6	11.000490862832574	27.297492418035684	31.987708368055877	29.714308351075864
7	14.1095377424787	32.276189426563256	27.033453820380682	26.58081901057737
8	13.789422984135152	28.534351261349265	23.81108456906228	33.865141185453304
9	10.83127046153815	27.72585187209213	26.120798792716343	35.32207887365338
10-11	13.37594441964502	44.999634110330064	24.981279103729868	16.643142366295045
12-13	12.672606639981637	45.128019699590475	25.62881454923397	16.57055911119392
14-15	13.260060176953242	44.30265004036672	25.36193220820669	17.075357574473347
16-17	13.252991395254757	43.9650006480076	25.633703607671226	17.148304349066414
18-19	13.423206825478642	43.0793004613296	26.333730674386434	17.163762038805324
20-21	12.846404778488843	42.797575622121464	26.984188462638215	17.37183113675148
22-23	12.984728952731569	42.98015482610149	27.46110743643949	16.574008784727447
24-25	13.013463960058171	42.92291688995696	27.170263100727077	16.893356049257797
26-27	13.831744598164972	43.17760325339369	26.49100755237594	16.499644596065398
28-29	13.550642593518608	43.55503521227668	26.945153983151087	15.949168211053626
30-31	13.628182649498921	43.33374943442211	27.006802004074913	16.03126591200406
32-33	13.850911979188627	43.46319954906882	26.666326334858393	16.019562136884165
34-35	13.945779085623695	43.438089691578476	26.608683070786316	16.00744815201152
36-37	14.209108290627245	43.3649613002693	26.41185768739842	16.014072721705034
38-39	14.429922047065538	43.33277865631036	26.44246962868148	15.794829667942622
40-41	14.640397071338132	43.594115145134424	26.281035590332745	15.484452193194699
42-43	14.744192840698608	43.58696164254524	26.407142446255843	15.261703070500307
44-45	14.794845756484804	43.43377277254664	26.48123167424787	15.290149796720687
46-47	14.877997067393691	43.25968914169942	26.600684647296756	15.261629143610131
48-49	14.871646545328387	43.18712753590515	26.82567328995786	15.1155526288086
50-51	15.018935755152734	43.2822333977273	26.89976496391538	14.799065883204584
52-53	15.110480776288208	43.215054971391545	27.03435715146189	14.64010710085836
54-55	15.121838561022946	43.318489355668824	26.99345936837318	14.566212714935055
56-57	15.055457382666185	43.38801780555402	26.781685561067796	14.774839250711999
58-59	14.975707096961365	43.232414952405314	26.936163398794342	14.855714551838977
60-61	14.965746904243293	43.02117460253228	27.204550170387147	14.808528322837281
62-63	14.998207033001936	42.630266501941705	27.414643173324716	14.956883291731646
64-65	14.998577509481084	42.583051480444	27.319762071213916	15.09860893886101
66-67	14.853724531573267	42.425779414706824	27.49962638780899	15.220869665910921
68-69	14.798083655664081	42.333577282182965	27.412918217375527	15.455420844777429
70-71	14.754659094054393	42.3322729949409	27.44322000908571	15.469847901918996
72-73	14.825550563169223	42.408484101641754	27.465011795136977	15.300953540052042
74-75	14.794575499937594	42.553582223530704	27.371989256912162	15.27985301961955
76-77	14.54680434487359	42.84358313716925	27.35999483319468	15.24961768476249
78-79	14.314701224167775	43.14578069733257	27.293032942033747	15.246485136465907
80-81	14.060387369334837	43.585584591223665	27.145131373198613	15.208896666242888
82-83	13.856922373210978	44.02225122292766	27.142637311888535	14.978189091972826
84-85	13.613590068789374	44.48059297661706	27.15650626815858	14.74931068643498
86-87	13.4590727516714	44.97910338559349	27.013305170980995	14.548518691754115
88-89	13.403060499419484	45.395365364036	26.836511679696017	14.365062456848493
90-91	13.420588287202634	45.73884772858928	26.616438841435087	14.224125142773003
92-93	13.516542162591557	46.04790588956261	26.316472416044924	14.119079531800912
94-95	13.679612034678518	46.093245332792165	26.102365912141522	14.124776720387796
96-97	13.883182389137469	45.96893036262583	26.0363903176418	14.111496930594903
98-99	14.100711531751259	45.729759810840605	25.960589873653888	14.208938783754247
100	14.450609963083075	45.48618006756957	25.827938857242987	14.235271112104368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	522074.0
1	464176.0
2	261148.5
3	85438.5
4	56948.5
5	57147.5
6	49829.0
7	39273.0
8	31923.5
9	30272.5
10	33208.0
11	40035.5
12	51042.5
13	66098.0
14	84889.0
15	106799.5
16	130548.0
17	155066.5
18	181392.5
19	208823.5
20	235631.0
21	261065.5
22	285279.0
23	308388.0
24	327220.5
25	340064.0
26	347998.5
27	352307.0
28	353188.5
29	349447.5
30	341261.5
31	328798.0
32	312581.0
33	296012.5
34	280327.5
35	263847.0
36	246141.0
37	229503.0
38	213706.5
39	197753.0
40	183028.0
41	170209.5
42	160813.5
43	156349.5
44	157357.5
45	163349.0
46	172578.5
47	182327.5
48	188700.5
49	191371.0
50	193785.0
51	198984.5
52	207699.0
53	215134.5
54	214742.5
55	201065.5
56	172329.0
57	133809.0
58	94413.0
59	60918.5
60	36644.5
61	21621.5
62	13165.5
63	8725.5
64	6342.5
65	5002.0
66	4257.5
67	3681.0
68	3243.5
69	2859.5
70	2535.5
71	2292.0
72	2085.5
73	1957.5
74	1769.5
75	1625.0
76	1565.5
77	1430.5
78	1331.5
79	1332.5
80	1288.5
81	1184.0
82	1113.5
83	1118.5
84	1107.0
85	1063.0
86	1056.5
87	1059.5
88	1050.0
89	1012.5
90	1013.0
91	993.5
92	949.0
93	923.0
94	868.5
95	846.5
96	837.5
97	797.5
98	718.0
99	630.5
100	588.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1530533138527959
2	0.1443371203827212
3	0.1457743200819576
4	0.14207141732745446
5	0.14048204354241658
6	0.12399651758058759
7	0.12545907962745753
8	0.1269639122537168
9	0.12621995005646502
10-11	0.12586065013165595
12-13	0.1294959199591362
14-15	0.13719761952386758
16-17	0.13040473741600625
18-19	0.13769641236066138
20-21	0.13911670382814204
22-23	0.13921392616073744
24-25	0.14016924125493574
26-27	0.14072721290287457
28-29	0.14116259987058438
30-31	0.13970003782371443
32-33	0.13996634247386708
34-35	0.14176706915585147
36-37	0.1429802347843245
38-39	0.1427012489603551
40-41	0.13927310497188244
42-43	0.13640293263134864
44-45	0.13625075854554713
46-47	0.13694399604753174
48-49	0.13889689681531764
50-51	0.13625075854554713
52-53	0.13645788438455475
54-55	0.13926042379806566
56-57	0.13580691746195944
58-59	0.13659737729653945
60-61	0.13814870756012695
62-63	0.13303396745402102
64-65	0.12487574563188512
66-67	0.1254379443377629
68-69	0.12348081651203807
70-71	0.12178153922058801
72-73	0.12504482794944236
74-75	0.12688359815287714
76-77	0.12411910226081657
78-79	0.11940170560097013
80-81	0.1200484454656265
82-83	0.12077549943112255
84-85	0.11602851336570358
86-87	0.12008226192913793
88-89	0.11904240567616105
90-91	0.12501523854386984
92-93	0.12134192519493922
94-95	0.12356113061287774
96-97	0.127073815760129
98-99	0.1306329985447085
100	0.13144459366898315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1828558E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.80613808043402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.68136137067687	83.92535604947317
2	2.9681822146093344	5.153128703385327
3	0.1874998396509612	0.4882841091240173
4	0.04815690317067575	0.1672125914463908
5	0.032075276837454494	0.1392165455060111
6	0.016827973191493865	0.08764628186847952
7	0.011099222887290972	0.06744364721777857
8	0.006910955110125745	0.04799306588458052
9	0.006171378384709744	0.048214217178874794
>10	0.03338189232222838	0.5611788804783542
>50	0.004456451593841396	0.27883450686408445
>100	0.002697135714624002	0.4968479178240939
>500	4.6880664600531743E-4	0.2663769530631877
>1k	4.3802920095304987E-4	0.8232769159356332
>5k	8.760535820627188E-5	0.5172789751346871
>10k+	1.8494464510212952E-4	6.931710639615418
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	344983	2.9165262578921287	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83901	0.7093087762684175	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70771	0.5983062347921022	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62348	0.5270972167528789	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37553	0.3174774135613149	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25939	0.219291311755837	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24269	0.2051729382398091	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23915	0.2021801812190463	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19306	0.16321516113798487	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17104	0.1445991979749349	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16583	0.1401946036025693	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12945	0.10943853003891092	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12710	0.10745181280761357	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12174	0.10292040669708005	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11830	0.10001219083509588	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015048326262592617	0.0	0.0	0.0	0.0
2	0.0031702934541978827	0.0	0.0	2.536234763358306E-5	0.0
3	0.00497102013618228	0.0	0.0	5.072469526716612E-5	0.0
4	0.007828511302899304	0.0	0.0	5.9178811145027145E-5	0.0
5	0.012985521988394528	0.0	0.0	5.9178811145027145E-5	0.0
6	0.02219205417938518	0.0	0.0	5.9178811145027145E-5	0.0
7	0.03204955329297113	0.0	0.0	5.9178811145027145E-5	0.0
8	0.06600128265846099	0.0	0.0	5.9178811145027145E-5	0.0
9	0.19313427722973503	0.0	0.0	7.608704290074919E-5	0.0
10-11	0.47327408801647675	0.0	0.0	7.608704290074919E-5	0.0
12-13	0.8339647148874783	0.0	0.0	7.608704290074919E-5	0.0
14-15	1.228277360604733	0.0	0.0	8.03141008396797E-5	0.0
16-17	1.6577591283738897	0.0	0.0	8.876821671754072E-5	0.0
18-19	2.1340090651793737	0.0	0.0	9.722233259540174E-5	0.0
20-21	2.7523769169496397	0.0	0.0	1.0567644847326275E-4	0.0
22-23	3.459217091381722	0.0	0.0	1.1413056435112378E-4	0.0
24-25	4.215564568394559	0.0	0.0	1.1835762229005429E-4	0.0
26-27	5.01216631815983	0.0	0.0	1.1835762229005429E-4	0.0
28-29	5.900985563920809	0.0	0.0	1.1835762229005429E-4	0.0
30-31	6.914841183515353	0.0	0.0	1.1835762229005429E-4	0.0
32-33	8.033908275209878	0.0	0.0	1.268117381679153E-4	0.0
34-35	9.035230668015492	0.0	0.0	1.4371996992363733E-4	0.0
36-37	9.818403054708781	0.0	0.0	1.5640114374042887E-4	0.0
38-39	10.415339722728671	0.0	0.0	1.648552596182899E-4	0.0
40-41	10.935009998682848	0.0	0.0	1.7330937549615093E-4	0.0
42-43	11.375452527687653	0.0	0.0	1.9021760725187298E-4	0.0
44-45	11.757396801875597	0.0	0.0	2.113528969465255E-4	0.0
46-47	12.1009974334995	0.0	0.0	2.1980701282438655E-4	0.0
48-49	12.428383070869671	0.0	0.0	2.493964183969001E-4	0.0
50-51	12.755176920128388	0.0	0.0	2.916669977862052E-4	0.0
52-53	13.094673078493592	0.0	0.0	3.339375771755103E-4	0.0
54-55	13.44335886081803	0.0	0.0	4.184787359541205E-4	0.0
56-57	13.805355648592162	0.0	4.22705793893051E-6	4.3538696770984255E-4	0.0
58-59	14.174758241875299	0.0	8.45411587786102E-6	4.480681415266341E-4	0.0
60-61	14.546578712299503	0.0	8.45411587786102E-6	4.7343048916021716E-4	0.0
62-63	14.912295311059893	0.0	8.45411587786102E-6	5.114740106105918E-4	0.0
64-65	15.265766122971202	0.0	8.45411587786102E-6	5.495175320609663E-4	0.0
66-67	15.602341384300605	0.0	8.45411587786102E-6	6.002422273281324E-4	0.0
68-69	15.917337514851768	0.0	8.45411587786102E-6	6.171504590838545E-4	0.0
70-71	16.22395561656797	0.0	8.45411587786102E-6	6.425128067174376E-4	0.0
72-73	16.522576124663715	0.0	8.45411587786102E-6	6.763292702288816E-4	0.0
74-75	16.815671868033277	0.0	8.45411587786102E-6	6.932375019846037E-4	0.0
76-77	17.107381136398875	0.0	8.45411587786102E-6	7.397351393128393E-4	0.0
78-79	17.395467816110806	0.0	8.45411587786102E-6	7.989139504578665E-4	0.0
80-81	17.68548626130083	0.0	8.45411587786102E-6	8.707739354196851E-4	0.0
82-83	17.97792258363192	0.0	8.45411587786102E-6	8.876821671754072E-4	0.0
84-85	18.271922917400413	0.0	8.45411587786102E-6	9.088174568700597E-4	0.0
86-87	18.569300670462113	0.0	8.45411587786102E-6	9.214986306868512E-4	0.0
88	18.795367956094058	0.0	8.45411587786102E-6	9.214986306868512E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCTGA	3145	0.0	37.809082	9
CCTGCTG	2205	0.0	35.809242	8
GCCTGCT	875	0.0	34.376	7
TTGCTGA	3905	0.0	33.70026	9
CAGTCCG	140	5.11136E-10	33.579124	1
CTTGCTG	2360	0.0	33.25821	8
TAGCCGA	360	0.0	30.028938	5
ACTTGCC	790	0.0	29.748993	3
ATGCGCC	350	0.0	29.545	3
CCCGACG	4925	0.0	29.39266	8
TGCCAAA	1885	0.0	27.92338	6
TTGCGCC	405	0.0	27.853872	3
CTAGCCG	380	0.0	27.211807	4
GCTTGCT	940	0.0	26.999102	7
TGTGCCG	420	0.0	26.85841	4
AGCCGAC	335	0.0	26.654474	6
TGAACCG	5780	0.0	26.06248	12-13
TGCGCCT	345	0.0	25.885279	4
TCGCGAC	400	0.0	25.847826	6
GCTGAAC	6575	0.0	25.69806	10-11
>>END_MODULE