FastQCFastQC Report
Thu 26 May 2016
SRR1248450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11828558
Sequences flagged as poor quality0
Sequence length100
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223820.18922002157828538No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181800.15369582665951334No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177410.14998446978913238No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175540.14840355011997236No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161250.13632261853050895No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157710.13332986150974616No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147540.1247320256619615No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147370.12458830569203787No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146960.12424168694104556No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140980.11918612564608468No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138860.11739385307997813No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138640.1172078625306652No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137690.1164047215222684No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134960.11409674788761233No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131990.11158587547188761No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131890.11150133431310899No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130950.11070664742059008No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130100.10998804757097187No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127570.10784915625387305No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123530.10443369343921718No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122420.10349528657677462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTT24450.047.2903251
TATCTCG25300.046.0754139
TGCCCTA43300.046.027756
CTTAGGC9050.045.1842161
GGCATAT9000.044.393755
GCCCTAC116500.044.3401347
TGCTCTA41250.043.4152956
GCATATC9950.040.1548166
GCCCCTA42150.038.585166
CCCCTAC307400.037.767517
TTAGGCA10800.037.4248052
AGGCATA10650.037.0693134
CGCCCTA54100.036.8392146
CCCGGAA11750.036.003646
CCCCCTA181550.034.227646
TAGGCAT11700.033.7425773
GCTTTTC25950.033.6892741
GACTTTT26750.033.384581
TATGCTC6450.032.789724
GTCCCTA17050.032.531156
Produced by FastQC (version 0.11.5)