##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11828558 Sequences flagged as poor quality 0 Sequence length 100 %GC 31 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62955890312243 34.0 31.0 34.0 31.0 34.0 2 32.88530385529665 34.0 31.0 34.0 31.0 34.0 3 32.983617445169564 34.0 33.0 34.0 31.0 34.0 4 36.35280251405116 37.0 37.0 37.0 35.0 37.0 5 36.25904915882393 37.0 37.0 37.0 35.0 37.0 6 36.15787697874923 37.0 37.0 37.0 35.0 37.0 7 36.19778311100981 37.0 37.0 37.0 35.0 37.0 8 35.816493269931975 37.0 35.0 37.0 35.0 37.0 9 37.5916727973097 39.0 37.0 39.0 34.0 39.0 10-11 37.867761903014724 39.0 38.0 39.0 35.0 39.0 12-13 38.01042819420592 39.0 39.0 39.0 35.0 39.0 14-15 39.55666751602351 41.0 39.0 41.0 37.0 41.0 16-17 39.55060020841086 41.0 39.0 41.0 37.0 41.0 18-19 39.454197798243875 41.0 39.0 41.0 36.5 41.0 20-21 39.23267489579034 41.0 39.0 41.0 35.5 41.0 22-23 38.99041671013491 40.5 38.5 41.0 35.0 41.0 24-25 38.776954299923965 40.0 38.0 41.0 35.0 41.0 26-27 38.64980279083892 40.0 38.0 41.0 35.0 41.0 28-29 38.54135237786382 40.0 38.0 41.0 35.0 41.0 30-31 38.48412207134631 40.0 37.0 41.0 35.0 41.0 32-33 38.42065617803962 40.0 37.0 41.0 35.0 41.0 34-35 38.34924147136109 40.0 37.0 41.0 35.0 41.0 36-37 38.27270500766028 40.0 37.0 41.0 35.0 41.0 38-39 38.17408466019273 40.0 37.0 41.0 34.0 41.0 40-41 38.05645941796118 40.0 36.0 41.0 34.0 41.0 42-43 37.90846217265029 40.0 36.0 41.0 33.5 41.0 44-45 37.76766386063289 40.0 35.0 41.0 33.0 41.0 46-47 37.618157471096644 40.0 35.0 41.0 33.0 41.0 48-49 37.46754617088575 39.0 35.0 41.0 33.0 41.0 50-51 37.220898861890014 39.0 35.0 41.0 33.0 41.0 52-53 36.912801163083444 39.0 35.0 41.0 33.0 41.0 54-55 36.69076653299582 38.0 35.0 41.0 32.0 41.0 56-57 36.4482462274776 37.0 35.0 40.0 32.0 41.0 58-59 36.193656022991135 37.0 35.0 40.0 32.0 41.0 60-61 35.894947338466785 36.0 35.0 40.0 31.0 41.0 62-63 35.59087680002922 35.5 35.0 39.5 31.0 41.0 64-65 35.32949912406906 35.0 35.0 39.0 31.0 41.0 66-67 35.073260493798145 35.0 35.0 39.0 30.0 41.0 68-69 34.84088453554524 35.0 34.0 38.5 30.0 40.5 70-71 34.684086428793776 35.0 34.0 37.0 30.0 40.0 72-73 34.51135146820094 35.0 34.0 37.0 30.0 39.5 74-75 34.28460261174693 35.0 34.0 36.5 30.0 39.0 76-77 34.071333758519 35.0 34.0 36.0 30.0 39.0 78-79 33.86569009510711 35.0 34.0 36.0 30.0 38.0 80-81 33.68138322524183 35.0 34.0 35.0 30.0 37.0 82-83 33.51771526165742 35.0 34.0 35.0 29.0 37.0 84-85 33.362120513760004 35.0 34.0 35.0 29.0 36.5 86-87 33.23738819220398 35.0 34.0 35.0 29.0 36.0 88-89 33.12197784379127 35.0 34.0 35.0 29.0 36.0 90-91 33.02490789663457 35.0 34.0 35.0 29.0 36.0 92-93 32.90734614481326 35.0 34.0 35.0 29.0 36.0 94-95 32.78649375519822 35.0 33.5 35.0 29.0 35.0 96-97 32.68735415593346 35.0 33.0 35.0 28.0 35.0 98-99 32.56686055899628 35.0 33.0 35.0 27.0 35.0 100 31.907415933539827 35.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1865975521299248 1101 2 0.1980730338348735 1101 3 0.18316568936230482 1101 4 0.10232212227010251 1101 5 0.11526971500975236 1101 6 -0.044464380587506014 1101 7 0.010334152074818803 1101 8 -0.0853518072411461 1101 9 -0.1289771058378264 1101 10-11 0.018004220643355495 1101 12-13 0.11980624849043409 1101 14-15 0.20255066884168826 1101 16-17 0.23522152282455266 1101 18-19 0.26487894424622027 1101 20-21 0.2797476934894192 1101 22-23 0.23671902894749763 1101 24-25 0.20291613556030086 1101 26-27 0.2471915864111125 1101 28-29 0.26959560380158365 1101 30-31 0.27229602451966173 1101 32-33 0.28333293191779774 1101 34-35 0.2861413131357651 1101 36-37 0.28648315775744493 1101 38-39 0.2765837917394478 1101 40-41 0.2666982655361707 1101 42-43 0.2971095189798163 1101 44-45 0.32077679154375005 1101 46-47 0.3627316509521208 1101 48-49 0.3426167387138719 1101 50-51 0.2877307270715832 1101 52-53 0.25408751018338904 1101 54-55 0.2585179304262084 1101 56-57 0.2759970437172754 1101 58-59 0.2995029886223719 1101 60-61 0.2774211438792449 1101 62-63 0.27156586756191103 1101 64-65 0.27043750400595457 1101 66-67 0.23191606915694507 1101 68-69 0.2577657211962361 1101 70-71 0.27252815199732794 1101 72-73 0.3049171079051831 1101 74-75 0.2922721603055507 1101 76-77 0.2907724188138374 1101 78-79 0.2950340755555061 1101 80-81 0.29611920082447085 1101 82-83 0.2623672095069054 1101 84-85 0.28456654375476376 1101 86-87 0.2711871499513592 1101 88-89 0.28245379282702743 1101 90-91 0.28582922871837013 1101 92-93 0.3027318383539708 1101 94-95 0.2681987325502462 1101 96-97 0.28959701847286823 1101 98-99 0.29094099468415635 1101 100 0.2508395900809006 1102 1 0.11908188606151526 1102 2 0.16064941023022072 1102 3 0.15417416722139876 1102 4 0.08371520560881862 1102 5 0.07926147243325232 1102 6 -0.056221706112424386 1102 7 -0.010691173097200135 1102 8 -0.08841492154053299 1102 9 -0.09090330674202818 1102 10-11 -0.010783439348251989 1102 12-13 0.0784960394592531 1102 14-15 0.14854541064633509 1102 16-17 0.1842119559688058 1102 18-19 0.2274493790255434 1102 20-21 0.1896085513590009 1102 22-23 0.1496630933966827 1102 24-25 0.14722462471386422 1102 26-27 0.1654981458428395 1102 28-29 0.1648280030113014 1102 30-31 0.18820937112860037 1102 32-33 0.16612215569600153 1102 34-35 0.16412878980919032 1102 36-37 0.19348627360021453 1102 38-39 0.17191894225447157 1102 40-41 0.16552633795502913 1102 42-43 0.1734967593549115 1102 44-45 0.1591968785385376 1102 46-47 0.1637591325442358 1102 48-49 0.1754519156335732 1102 50-51 0.12715728651770064 1102 52-53 0.1299006924720274 1102 54-55 0.1785528079819514 1102 56-57 0.2060472386872263 1102 58-59 0.18768419298974237 1102 60-61 0.24351357717730338 1102 62-63 0.1779489744239413 1102 64-65 0.21749318382458682 1102 66-67 0.18312649589307028 1102 68-69 0.16006282995284948 1102 70-71 0.20005787479666992 1102 72-73 0.14242240176371723 1102 74-75 0.14932413717603055 1102 76-77 0.17419652808492003 1102 78-79 0.1766277064128019 1102 80-81 0.16557559778986075 1102 82-83 0.16332808836989443 1102 84-85 0.11054753175202592 1102 86-87 0.14817186431265839 1102 88-89 0.14886607563052223 1102 90-91 0.1611138139236843 1102 92-93 0.16652229993888312 1102 94-95 0.16379745351177633 1102 96-97 0.12170093167421925 1102 98-99 0.18589745931296164 1102 100 0.1493953099672325 1103 1 0.140761721616542 1103 2 0.16472274091875505 1103 3 0.1652099027122489 1103 4 0.09282637493664225 1103 5 0.1066929247544266 1103 6 0.03032051480965947 1103 7 0.025171740561511058 1103 8 -0.08971290942388777 1103 9 -0.16314570445147325 1103 10-11 -0.02699905029985672 1103 12-13 0.07514383355774612 1103 14-15 0.18472906028678437 1103 16-17 0.21840216033285742 1103 18-19 0.18681505586333458 1103 20-21 0.1585842065308114 1103 22-23 0.10649481948113504 1103 24-25 0.13095696517947175 1103 26-27 0.1111691722514152 1103 28-29 0.1345307312603481 1103 30-31 0.1349769742272784 1103 32-33 0.1648464206793463 1103 34-35 0.15583843861211477 1103 36-37 0.1294884398631453 1103 38-39 0.1298961003192005 1103 40-41 0.14826160723350057 1103 42-43 0.17630587592569924 1103 44-45 0.1795225326083596 1103 46-47 0.17938923555108488 1103 48-49 0.188981349693627 1103 50-51 0.17741214466371247 1103 52-53 0.0822363000975983 1103 54-55 0.1499450426623241 1103 56-57 0.1597356002083572 1103 58-59 0.12407247633572638 1103 60-61 0.16248185229503065 1103 62-63 0.13807246343299795 1103 64-65 0.10484663882529333 1103 66-67 0.06792804256539853 1103 68-69 0.05655392312920071 1103 70-71 0.11732312729804306 1103 72-73 0.10652202013811518 1103 74-75 0.09383251704378637 1103 76-77 0.08926656095788843 1103 78-79 0.14525944605517793 1103 80-81 0.1031185465322153 1103 82-83 0.10940260086889708 1103 84-85 0.06738137417551826 1103 86-87 0.12566563384392282 1103 88-89 0.11260511908868409 1103 90-91 0.07753071084135854 1103 92-93 0.11172532739735885 1103 94-95 0.06874174746100437 1103 96-97 0.06988620642928822 1103 98-99 0.060432929155254556 1103 100 0.029236024356467993 1104 1 -0.24455602013736666 1104 2 -0.22313787298795518 1104 3 -0.18342888298327864 1104 4 -0.23496050261973522 1104 5 -0.1089897462651237 1104 6 -0.060017208932315214 1104 7 0.026626673204390272 1104 8 -0.1775083238859665 1104 9 -0.04578595361654436 1104 10-11 0.066488181866454 1104 12-13 0.11369837594207866 1104 14-15 0.14150009297093646 1104 16-17 0.15886943865474024 1104 18-19 0.16581703529971037 1104 20-21 0.1749539577409891 1104 22-23 0.15703320741314286 1104 24-25 0.18683213873194404 1104 26-27 0.18518850693563849 1104 28-29 0.1896820033531057 1104 30-31 0.19988175489447713 1104 32-33 0.21337605593770093 1104 34-35 0.2540302563163479 1104 36-37 0.24621448962111003 1104 38-39 0.22437483310466888 1104 40-41 0.20957699430243792 1104 42-43 0.21492876046171006 1104 44-45 0.20711034078127 1104 46-47 0.2088708296071431 1104 48-49 0.20444853420336528 1104 50-51 0.18362114875392166 1104 52-53 0.1728905929416058 1104 54-55 0.20431448429241073 1104 56-57 0.19458282712118802 1104 58-59 0.17994492933584638 1104 60-61 0.19344295777742104 1104 62-63 0.12808803624999854 1104 64-65 0.12095864115183019 1104 66-67 0.15840592166029666 1104 68-69 0.14098617199407215 1104 70-71 0.1261042260259444 1104 72-73 0.11810875885251448 1104 74-75 0.12934454376148352 1104 76-77 0.1332971531001803 1104 78-79 0.13440631835807437 1104 80-81 0.13434912741704608 1104 82-83 0.12210879997580548 1104 84-85 0.11717456528963055 1104 86-87 0.12175559031508243 1104 88-89 0.1214350470409542 1104 90-91 0.07805868562465434 1104 92-93 0.12116409620669799 1104 94-95 0.13750886817847885 1104 96-97 0.16073323633660408 1104 98-99 0.1776545415984998 1104 100 0.1576843713863738 1105 1 0.11140710223894956 1105 2 0.1170171122220296 1105 3 0.10430115934983064 1105 4 0.06006429501385924 1105 5 0.06081594656304645 1105 6 0.030241837775797364 1105 7 0.05508364045379466 1105 8 -0.17302916275448865 1105 9 -0.05767305353329277 1105 10-11 0.022870605658013687 1105 12-13 0.08057945357638374 1105 14-15 0.12723981523743788 1105 16-17 0.12779756094250416 1105 18-19 0.13316935653533335 1105 20-21 0.16640275948390837 1105 22-23 0.10676964399318933 1105 24-25 0.07400396862018255 1105 26-27 0.12059301833370029 1105 28-29 0.09770474632563975 1105 30-31 0.10967540315775892 1105 32-33 0.13103387516295584 1105 34-35 0.1376656336831914 1105 36-37 0.11997996600136673 1105 38-39 0.12354592792019758 1105 40-41 0.14202536208676975 1105 42-43 0.12300123154589215 1105 44-45 0.09898823014840019 1105 46-47 0.13895850695450918 1105 48-49 0.08492252325697791 1105 50-51 0.08986554714561379 1105 52-53 -0.13183105362126213 1105 54-55 -0.04914133163829604 1105 56-57 -0.010277066248981725 1105 58-59 -0.008550474245645034 1105 60-61 0.012844493459439832 1105 62-63 -0.045581083611132556 1105 64-65 -0.025378615521390202 1105 66-67 -0.010478981894614492 1105 68-69 -0.01967158523709145 1105 70-71 -0.014407756544862593 1105 72-73 -0.04466689341381169 1105 74-75 -0.014752600968130025 1105 76-77 -0.01853955448257949 1105 78-79 0.011552642108895839 1105 80-81 0.0027091624879957976 1105 82-83 -0.01018985209567802 1105 84-85 -0.03683685047812446 1105 86-87 -0.02650742401441164 1105 88-89 0.013898647657356378 1105 90-91 0.01241120169883203 1105 92-93 0.031125748437595746 1105 94-95 0.012017786470281067 1105 96-97 0.013967821950437553 1105 98-99 -0.006305773331447995 1105 100 -0.03213460588836625 1106 1 0.10182574624439411 1106 2 0.09741336589662097 1106 3 0.07623398501292655 1106 4 0.055055900729996665 1106 5 0.06586823525952923 1106 6 0.04725163333652205 1106 7 0.03883033374138023 1106 8 -0.07446981918079842 1106 9 -0.08427900377625974 1106 10-11 0.0664076759526111 1106 12-13 0.11783979008349377 1106 14-15 0.1490471446846513 1106 16-17 0.11355842251040116 1106 18-19 0.11516219631153035 1106 20-21 0.10773691552395093 1106 22-23 0.08755250293243222 1106 24-25 0.08999863273177056 1106 26-27 0.11032059706773367 1106 28-29 0.09936408053518875 1106 30-31 0.10888028722634147 1106 32-33 0.1048737249353735 1106 34-35 0.09895795207737734 1106 36-37 0.10918587009248881 1106 38-39 0.07790673330324438 1106 40-41 0.10524305663516031 1106 42-43 0.12331626801587703 1106 44-45 0.10004934622027406 1106 46-47 0.07215560039191615 1106 48-49 0.10596303821786535 1106 50-51 0.07769630199574351 1106 52-53 0.027670874388554978 1106 54-55 0.06402958400750691 1106 56-57 0.06016385103554711 1106 58-59 0.021529363420270897 1106 60-61 0.06923485106931082 1106 62-63 0.08302954535817264 1106 64-65 0.087248678275202 1106 66-67 0.05453257278694679 1106 68-69 0.05056097990221531 1106 70-71 0.06787319779491696 1106 72-73 0.05397058221433326 1106 74-75 0.09594689203049711 1106 76-77 0.10943092673395682 1106 78-79 0.12108291336799937 1106 80-81 0.0730942711621978 1106 82-83 0.07773320643354964 1106 84-85 0.08774004918844724 1106 86-87 0.07556267828883279 1106 88-89 0.06085877146468022 1106 90-91 0.09825617332214165 1106 92-93 0.12292702463629013 1106 94-95 0.09039456273083601 1106 96-97 0.07457442601112518 1106 98-99 0.10014202325264421 1106 100 0.06158966362499996 1107 1 0.049676606606006146 1107 2 0.02931304056680517 1107 3 0.0670797683367752 1107 4 0.02657066528192331 1107 5 0.007839720681040774 1107 6 0.003265853934436791 1107 7 -0.03745754203901441 1107 8 -0.17074024552605493 1107 9 -0.12114082020972461 1107 10-11 -0.05161331690279525 1107 12-13 -0.005114963890093804 1107 14-15 0.023069430959651527 1107 16-17 0.04272525458880949 1107 18-19 0.06541027883855577 1107 20-21 0.022079732664373353 1107 22-23 0.03470711519327807 1107 24-25 0.05169585946856614 1107 26-27 0.07023748719062439 1107 28-29 0.05928267490028105 1107 30-31 0.04848246997965333 1107 32-33 0.0721826102126073 1107 34-35 0.09455861123816334 1107 36-37 0.09651136571619645 1107 38-39 0.12603936003770144 1107 40-41 0.08496567718223247 1107 42-43 0.0898619986485727 1107 44-45 0.10262589977739367 1107 46-47 0.11891937979159906 1107 48-49 0.10520257527350907 1107 50-51 0.10143012522853212 1107 52-53 0.052393473883213915 1107 54-55 0.0961090411923351 1107 56-57 0.13061108328606252 1107 58-59 0.1489233525379916 1107 60-61 0.16618019742546153 1107 62-63 0.07400207417691007 1107 64-65 0.07314345254732757 1107 66-67 0.07007593331999118 1107 68-69 0.08828934835697311 1107 70-71 0.09362279870149592 1107 72-73 0.07046466929590167 1107 74-75 0.06089938678042728 1107 76-77 0.08097685886821893 1107 78-79 0.08038497305497572 1107 80-81 0.06510327998696397 1107 82-83 0.07571496262104915 1107 84-85 0.08385399602490651 1107 86-87 0.07306921155142732 1107 88-89 0.1050479421483459 1107 90-91 0.04236725963820476 1107 92-93 0.06419158036842276 1107 94-95 0.08275804892066674 1107 96-97 0.08287973959770767 1107 98-99 0.11371364072285672 1107 100 0.047759798324293 1108 1 0.03783000171091544 1108 2 0.022039787648651554 1108 3 0.04661855710693885 1108 4 0.013925270147232993 1108 5 -0.0031336152170808873 1108 6 -0.001537459556061549 1108 7 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2214 28-29 -0.20866481695066597 2214 30-31 -0.24876896508327917 2214 32-33 -0.23008077903021018 2214 34-35 -0.2434095287890159 2214 36-37 -0.24921749703317886 2214 38-39 -0.2627368811643862 2214 40-41 -0.2709584933586129 2214 42-43 -0.2677898002106005 2214 44-45 -0.2402173219704764 2214 46-47 -0.26221384569655726 2214 48-49 -0.23543374909701242 2214 50-51 -0.23785997397190783 2214 52-53 -0.19309743134615331 2214 54-55 -0.17947680063581828 2214 56-57 -0.19572107255279292 2214 58-59 -0.18568169347294372 2214 60-61 -0.1974682031110433 2214 62-63 -0.21278718180617773 2214 64-65 -0.15979759334879873 2214 66-67 -0.1997623392268153 2214 68-69 -0.16469660728598967 2214 70-71 -0.17389197340632023 2214 72-73 -0.14124349659945068 2214 74-75 -0.162041934737843 2214 76-77 -0.1393510017746351 2214 78-79 -0.15947700981814705 2214 80-81 -0.17796057512966712 2214 82-83 -0.1892883178709397 2214 84-85 -0.16801057505109895 2214 86-87 -0.12432899529971309 2214 88-89 -0.12561056649848013 2214 90-91 -0.1139013310574839 2214 92-93 -0.12450148522410842 2214 94-95 -0.1377599743412432 2214 96-97 -0.10840694967820497 2214 98-99 -0.10558177075211717 2214 100 -0.11722679174430795 2215 1 -0.2538056307855783 2215 2 -0.24060423027854227 2215 3 -0.2513862141270451 2215 4 -0.16191839223608184 2215 5 -0.18070003252110212 2215 6 -0.10710851421237777 2215 7 -0.10628802454171904 2215 8 0.18301956012903275 2215 9 0.07413859995167371 2215 10-11 -0.08902980698957919 2215 12-13 -0.18934222052658356 2215 14-15 -0.295018588158392 2215 16-17 -0.37922856469497646 2215 18-19 -0.40978347369707535 2215 20-21 -0.4143869241137992 2215 22-23 -0.3811202387449484 2215 24-25 -0.3862200621098637 2215 26-27 -0.4422876831843823 2215 28-29 -0.41787508633991166 2215 30-31 -0.42559547183486046 2215 32-33 -0.4306507432664688 2215 34-35 -0.4713874914213747 2215 36-37 -0.43220587235555286 2215 38-39 -0.4371877529447019 2215 40-41 -0.4447367839030605 2215 42-43 -0.536148133123227 2215 44-45 -0.5481624257024009 2215 46-47 -0.5444891807644865 2215 48-49 -0.5205739085822785 2215 50-51 -0.5000484537720595 2215 52-53 -0.43037870441900594 2215 54-55 -0.4545251351816688 2215 56-57 -0.45609972969702284 2215 58-59 -0.4811930125597428 2215 60-61 -0.4069079865564973 2215 62-63 -0.4138060847180469 2215 64-65 -0.40875906702740394 2215 66-67 -0.46007283892948436 2215 68-69 -0.3876268948791406 2215 70-71 -0.39331444308071895 2215 72-73 -0.4047859782082952 2215 74-75 -0.4247009930841372 2215 76-77 -0.43036705445881296 2215 78-79 -0.41863416453233526 2215 80-81 -0.44882531548162063 2215 82-83 -0.4469533719284797 2215 84-85 -0.45789577843140705 2215 86-87 -0.42311743335407215 2215 88-89 -0.45379045703872123 2215 90-91 -0.4311706637714181 2215 92-93 -0.4153831027596837 2215 94-95 -0.3949100840427491 2215 96-97 -0.45207491531308364 2215 98-99 -0.44207971576317107 2215 100 -0.3973953550063847 2216 1 -0.19660113531202938 2216 2 -0.20564609392094013 2216 3 -0.226049218674774 2216 4 -0.12398129362444621 2216 5 -0.15761846200443586 2216 6 -0.08303402614609467 2216 7 -0.09647063979488735 2216 8 0.19832882412346464 2216 9 0.14361483624580273 2216 10-11 -0.071485959933419 2216 12-13 -0.22835255304374869 2216 14-15 -0.34992959459281536 2216 16-17 -0.3588223967144373 2216 18-19 -0.42271749669869507 2216 20-21 -0.3736473616881497 2216 22-23 -0.2844627779972555 2216 24-25 -0.25138701950979225 2216 26-27 -0.24248590739937725 2216 28-29 -0.22599460187075948 2216 30-31 -0.24904448518125122 2216 32-33 -0.28072451687569355 2216 34-35 -0.2829101082606158 2216 36-37 -0.31001314250539735 2216 38-39 -0.29819827812067246 2216 40-41 -0.30476424989394957 2216 42-43 -0.34913544033305044 2216 44-45 -0.3724932796931313 2216 46-47 -0.34380523707420707 2216 48-49 -0.30023195097447797 2216 50-51 -0.3010180685643036 2216 52-53 -0.2803584503294658 2216 54-55 -0.3060623118692547 2216 56-57 -0.2681854241660062 2216 58-59 -0.275723658948408 2216 60-61 -0.25567132339072884 2216 62-63 -0.25191561523140393 2216 64-65 -0.25970543887884645 2216 66-67 -0.2794335437138642 2216 68-69 -0.23952681014426958 2216 70-71 -0.23601769265413708 2216 72-73 -0.24145496051788484 2216 74-75 -0.21272343907043734 2216 76-77 -0.20286530314324125 2216 78-79 -0.23888737906263202 2216 80-81 -0.24075809150048144 2216 82-83 -0.2288685526797778 2216 84-85 -0.2686363899705455 2216 86-87 -0.27516882198000303 2216 88-89 -0.28003670395656854 2216 90-91 -0.3083628218047849 2216 92-93 -0.2684598216084524 2216 94-95 -0.24804789093798263 2216 96-97 -0.276568196512855 2216 98-99 -0.267375486226193 2216 100 -0.1653270196036445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 63.0 8 259.0 9 526.0 10 901.0 11 1442.0 12 2204.0 13 3041.0 14 4011.0 15 5408.0 16 7491.0 17 10370.0 18 14062.0 19 18427.0 20 24523.0 21 32173.0 22 39212.0 23 47280.0 24 56292.0 25 68020.0 26 79451.0 27 94366.0 28 113377.0 29 140969.0 30 165192.0 31 202165.0 32 260882.0 33 363242.0 34 512631.0 35 1696651.0 36 1484217.0 37 2976087.0 38 2575381.0 39 819002.0 40 9235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.828715424869703 22.49809662668561 32.41752718304554 25.255660765399146 2 16.6640811024564 24.040125138489724 37.05858606695104 22.237207692102842 3 14.077466306306885 21.435909002870936 40.128725847946356 24.357898842875827 4 14.16979510094125 19.52185259672163 42.96890244459197 23.339449857745155 5 12.647885373283344 20.109595170568042 44.92761931383842 22.314900142310197 6 11.95452321485686 16.601240016168756 42.029442795920005 29.414793973054383 7 15.856083218258727 17.914389902809795 39.0247991344338 27.20472774449768 8 9.667628125085068 18.418229846782676 41.92058744607753 29.99355458205472 9 11.625381555384857 16.32110186212047 42.550765697729176 29.5027508847655 10-11 16.088851156713268 28.11811704155618 40.45146542492072 15.341566376809828 12-13 14.12587151855466 31.39734736071048 39.11322770414878 15.36355341658608 14-15 14.732025385122085 30.180240825817506 38.98206392575782 16.10566986330259 16-17 17.625586067044754 30.201090473487767 38.996218592307926 13.177104867159544 18-19 17.647001385249684 27.432170806007683 39.03541544619129 15.885412362551346 20-21 14.695647564543071 26.74813572487639 42.20582682882249 16.350389881758044 22-23 14.537926148582486 27.028297430432602 43.18010727859866 15.253669142386247 24-25 14.623816715469633 27.707951699097332 42.2559347258777 15.412296859555333 26-27 16.615441194342786 27.978644369788043 39.986223674018056 15.419690761851118 28-29 15.60485456822262 29.525182011645906 41.43876955426803 13.431193865863445 30-31 15.489457188976571 28.089081631954286 41.71989425020356 14.701566928865583 32-33 15.896198015447602 28.658114669725204 41.513062369563464 13.932624945263733 34-35 15.925281142389593 28.82682903713894 41.32441638283368 13.923473437637778 36-37 16.09279657692583 28.398979678794284 41.18400055053789 14.324223193741995 38-39 16.294984422274876 28.4013057356396 40.974616407184456 14.329093434901072 40-41 16.46580026859589 28.75052611053231 40.85363629296759 13.93003732790421 42-43 16.524279629013105 28.872775531894927 40.94286049068703 13.660084348404936 44-45 16.62774650487873 28.55431733962954 40.93328750048188 13.884648655009837 46-47 16.778421728383037 28.319074185527604 41.073337240829325 13.82916684526003 48-49 16.486358565984276 28.306281517353398 41.207651500462426 13.999708416199901 50-51 16.512217091098556 28.399813882482615 41.13268594182711 13.955283084591713 52-53 16.5244625164333 28.356991504370445 41.20058963246497 13.917956346731291 54-55 16.463011849249657 28.378588761750727 41.31444551516614 13.843953873833476 56-57 16.364694974534892 28.53273824142411 41.057347159669874 14.04521962437113 58-59 16.48465797954865 28.656215843913735 40.98743455484104 13.871691621696577 60-61 16.366418800468054 28.397551303407987 41.1157897575758 14.120240138548168 62-63 16.304233542944942 28.305938112540062 41.177734144215805 14.21209420029919 64-65 16.385979503272758 28.316728492138594 41.01333345058343 14.283958554005213 66-67 16.242638861231992 28.297365273562303 41.15132459276715 14.308671272438552 68-69 16.187566186868594 28.15221144892885 41.34481231932317 14.315410044879389 70-71 16.142073134383256 28.08319138618629 41.52886906913535 14.245866410295111 72-73 16.1483131208849 27.875012577292217 41.7001455161002 14.27652878572268 74-75 16.2081221048945 27.738551492952478 41.74464843446629 14.308677967686725 76-77 16.1429651709458 27.778847133925183 41.85230165628917 14.225886038839853 78-79 16.11583243730845 27.84594209063021 41.92381379418486 14.114411677876474 80-81 16.02510822876856 27.731216389894136 42.099084283153516 14.144591098183787 82-83 16.07365177746776 27.620152158398554 42.206387673104096 14.099808391029589 84-85 16.02434842854225 27.44957085142177 42.36667405233185 14.159406667704127 86-87 15.98746640764563 27.525899003440912 42.42483108279497 14.061803506118492 88-89 16.00139795207715 27.754895220811576 42.30460947937319 13.939097347738086 90-91 16.046062795701545 27.876648274927955 42.20855841082797 13.86873051854253 92-93 15.982402262245 28.01241559637534 42.07828286906249 13.926899272317167 94-95 16.00647516768037 28.240224966927258 41.96118171471967 13.792118150672698 96-97 15.843866477757423 28.59136387839534 42.0251495151042 13.539620128743035 98-99 15.735540724329658 29.022507359482457 41.860532459760634 13.381419456427249 100 15.791074769452626 29.35184803150352 41.58704152625947 13.270035672784383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 129192.0 1 303805.5 2 481938.5 3 343586.0 4 124114.0 5 32915.0 6 16095.5 7 12164.5 8 12325.0 9 15210.5 10 20497.0 11 28391.0 12 39997.5 13 55609.0 14 74604.5 15 96985.0 16 122889.5 17 151132.0 18 179232.5 19 206358.0 20 231116.5 21 254315.0 22 277491.0 23 300764.0 24 319909.0 25 332645.5 26 341114.0 27 346108.0 28 347896.5 29 345417.5 30 338932.0 31 327577.5 32 312464.0 33 296720.5 34 280704.5 35 264256.0 36 246622.5 37 228666.0 38 210784.0 39 191037.5 40 170838.0 41 151560.5 42 133506.5 43 117794.0 44 105113.0 45 96100.0 46 93069.0 47 97318.5 48 108520.0 49 126492.5 50 151957.5 51 186170.5 52 229245.5 53 275677.0 54 312527.0 55 325277.0 56 304737.0 57 251804.0 58 180819.5 59 112587.5 60 61176.5 61 29666.0 62 13497.0 63 6285.5 64 3244.0 65 1903.5 66 1195.0 67 839.5 68 649.0 69 484.5 70 368.5 71 284.0 72 207.5 73 156.0 74 121.5 75 80.5 76 58.0 77 52.5 78 41.0 79 29.0 80 22.0 81 17.0 82 14.5 83 15.0 84 11.0 85 6.5 86 4.0 87 2.0 88 4.0 89 6.5 90 4.5 91 3.0 92 2.0 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007380443161372671 2 5.326093003052443E-4 3 7.608704290074919E-5 4 0.0041425167801519 5 0.01333214073938683 6 0.006839379745189566 7 0.0 8 0.0 9 0.0 10-11 0.011328515276333767 12-13 0.005410634161831053 14-15 0.0011286244696944463 16-17 2.2403407076331707E-4 18-19 3.8043521450374594E-5 20-21 8.45411587786102E-4 22-23 8.876821671754072E-5 24-25 3.550728668701629E-4 26-27 0.0024981912419079315 28-29 0.001640098480305038 30-31 0.0022065242441217264 32-33 0.0021008477956484637 34-35 0.002527780647480445 36-37 0.0010652186006104886 38-39 0.0010990350641219327 40-41 6.340586908395765E-5 42-43 0.0 44-45 5.917881114502714E-4 46-47 9.299527465647123E-5 48-49 4.607493153434256E-4 50-51 0.03188047097541391 52-53 0.035870813669764315 54-55 7.397351393128393E-4 56-57 1.7753643343508145E-4 58-59 4.015705041983985E-4 60-61 0.00396920740465575 62-63 0.012896753771676987 64-65 0.01679832824930985 66-67 0.017694464532363115 68-69 0.018548330236027078 70-71 0.016679970627019796 72-73 0.014270547601829404 74-75 0.015289268565111657 76-77 0.013589991273661589 78-79 0.01508636978404299 80-81 0.014862335713279674 82-83 0.01358153715778373 84-85 0.013471633651371537 86-87 0.01235991741343281 88-89 0.010626823658471304 90-91 0.008158221822135886 92-93 0.008580927616028935 94-95 0.010309794313051516 96-97 0.00554590001587683 98-99 0.00811595124274658 100 0.008910638135265516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1828558E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.4724494841678 #Duplication Level Percentage of deduplicated Percentage of total 1 96.08621287802306 81.16637763463797 2 3.43467778604983 5.802712915529751 3 0.24100336601112662 0.6107443398266789 4 0.07248080894496672 0.24490525888701278 5 0.041798536415206186 0.17654123779228265 6 0.023028519556545956 0.11671652729612988 7 0.014990746869249111 0.08864135753498144 8 0.011942977764890327 0.08070820687481899 9 0.008704207369273322 0.06617391455705955 >10 0.05472138061354422 0.8702923056475211 >50 0.004381002057800441 0.25369657011433877 >100 0.003339565337459983 0.6221944621897973 >500 7.739858280017993E-4 0.4396397520847025 >1k 0.0010522959682724945 2.2447144272870605 >5k 5.411790146694238E-4 3.295573946984001 >10k+ 3.507641761746265E-4 3.920367142755902 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22382 0.18922002157828538 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18180 0.15369582665951334 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17741 0.14998446978913238 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17554 0.14840355011997236 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16125 0.13632261853050895 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15771 0.13332986150974616 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14754 0.1247320256619615 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14737 0.12458830569203787 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14696 0.12424168694104556 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14098 0.11918612564608468 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13886 0.11739385307997813 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13864 0.1172078625306652 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13769 0.1164047215222684 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13496 0.11409674788761233 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13199 0.11158587547188761 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13189 0.11150133431310899 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13095 0.11070664742059008 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13010 0.10998804757097187 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12757 0.10784915625387305 No Hit ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12353 0.10443369343921718 No Hit GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12242 0.10349528657677462 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010483103688547666 0.0 0.0 0.0 0.0 2 0.0022741571711446147 0.0 0.0 8.45411587786102E-6 0.0 3 0.003677540406869544 0.0 0.0 1.690823175572204E-5 0.0 4 0.006154596359082823 0.0 0.0 1.690823175572204E-5 0.0 5 0.010618369542593441 0.0 0.0 2.536234763358306E-5 0.0 6 0.02012079578930923 0.0 0.0 2.536234763358306E-5 0.0 7 0.029766942005948652 0.0 0.0 3.381646351144408E-5 0.0 8 0.07678873451861165 0.0 0.0 3.381646351144408E-5 0.0 9 0.27630586923613176 0.0 0.0 3.381646351144408E-5 0.0 10-11 0.6651106584589601 0.0 0.0 4.22705793893051E-5 0.0 12-13 1.044882224866294 0.0 0.0 4.22705793893051E-5 0.0 14-15 1.4141030546580573 0.0 0.0 4.22705793893051E-5 0.0 16-17 1.779037647699745 0.0 0.0 5.495175320609664E-5 0.0 18-19 2.178541120566006 0.0 0.0 5.9178811145027145E-5 0.0 20-21 2.703583987160565 0.0 0.0 6.340586908395765E-5 0.0 22-23 3.332384217924112 0.0 0.0 6.763292702288816E-5 0.0 24-25 4.076126608163058 0.0 0.0 6.763292702288816E-5 0.0 26-27 4.903382982101453 0.0 0.0 6.763292702288816E-5 0.0 28-29 5.857713171799977 0.0 0.0 6.763292702288816E-5 0.0 30-31 6.933292291418785 0.0 0.0 7.185998496181867E-5 0.0 32-33 8.13899293557169 0.0 0.0 7.608704290074919E-5 0.0 34-35 9.232097437405304 0.0 0.0 7.608704290074919E-5 0.0 36-37 10.053123127941715 0.0 0.0 8.876821671754072E-5 0.0 38-39 10.650774168753284 0.0 4.22705793893051E-6 1.1413056435112378E-4 0.0 40-41 11.212275410071118 0.0 8.45411587786102E-6 1.268117381679153E-4 0.0 42-43 11.68236229640164 0.0 8.45411587786102E-6 1.310387961068458E-4 0.0 44-45 12.070947278611644 0.0 8.45411587786102E-6 1.4371996992363736E-4 0.0 46-47 12.40964875008433 0.0 8.45411587786102E-6 1.4371996992363736E-4 0.0 48-49 12.72884657622679 0.0 8.45411587786102E-6 1.5217408580149838E-4 0.0 50-51 13.041741858982304 0.0 8.45411587786102E-6 1.8176349137401194E-4 0.0 52-53 13.371794769911936 0.0 8.45411587786102E-6 2.028987810686645E-4 0.0 54-55 13.70022871765096 0.0 8.45411587786102E-6 2.1980701282438655E-4 0.0 56-57 14.034221246579676 8.45411587786102E-6 8.45411587786102E-6 2.2826112870224756E-4 0.0 58-59 14.356259655657098 8.45411587786102E-6 8.45411587786102E-6 2.2826112870224756E-4 0.0 60-61 14.672862068225054 8.45411587786102E-6 8.45411587786102E-6 2.4094230251903907E-4 0.0 62-63 14.993361828212704 8.45411587786102E-6 8.45411587786102E-6 2.705317080915527E-4 0.0 64-65 15.309630303203484 8.45411587786102E-6 8.45411587786102E-6 2.9589405572513577E-4 0.0 66-67 15.614194054761366 8.45411587786102E-6 8.45411587786102E-6 3.254834612976493E-4 0.0 68-69 15.899998123186275 8.45411587786102E-6 8.45411587786102E-6 3.6775404068695443E-4 0.0 70-71 16.183650619120268 8.45411587786102E-6 8.45411587786102E-6 3.9734344625946794E-4 0.0 72-73 16.467281979764564 8.45411587786102E-6 8.45411587786102E-6 4.05797562137329E-4 0.0 74-75 16.74823761273352 8.45411587786102E-6 8.45411587786102E-6 4.184787359541205E-4 0.0 76-77 17.030621991285834 8.45411587786102E-6 8.45411587786102E-6 4.607493153434256E-4 0.0 78-79 17.30912593065021 8.45411587786102E-6 8.45411587786102E-6 5.326093003052443E-4 0.0 80-81 17.589536273145043 8.45411587786102E-6 8.45411587786102E-6 6.086963432059935E-4 0.0 82-83 17.87309154674644 8.45411587786102E-6 8.45411587786102E-6 6.425128067174376E-4 0.0 84-85 18.159487403282803 8.45411587786102E-6 8.45411587786102E-6 6.467398646563681E-4 0.0 86-87 18.447045700752366 8.45411587786102E-6 8.45411587786102E-6 6.594210384731596E-4 0.0 88 18.665267566849653 8.45411587786102E-6 8.45411587786102E-6 6.594210384731596E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTTT 2445 0.0 47.290325 1 TATCTCG 2530 0.0 46.075413 9 TGCCCTA 4330 0.0 46.02775 6 CTTAGGC 905 0.0 45.184216 1 GGCATAT 900 0.0 44.39375 5 GCCCTAC 11650 0.0 44.340134 7 TGCTCTA 4125 0.0 43.415295 6 GCATATC 995 0.0 40.154816 6 GCCCCTA 4215 0.0 38.58516 6 CCCCTAC 30740 0.0 37.76751 7 TTAGGCA 1080 0.0 37.424805 2 AGGCATA 1065 0.0 37.069313 4 CGCCCTA 5410 0.0 36.839214 6 CCCGGAA 1175 0.0 36.00364 6 CCCCCTA 18155 0.0 34.22764 6 TAGGCAT 1170 0.0 33.742577 3 GCTTTTC 2595 0.0 33.689274 1 GACTTTT 2675 0.0 33.38458 1 TATGCTC 645 0.0 32.78972 4 GTCCCTA 1705 0.0 32.53115 6 >>END_MODULE