FastQCFastQC Report
Thu 26 May 2016
SRR1248449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17640105
Sequences flagged as poor quality0
Sequence length100
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10301525.839829184690227No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2443171.385008762702943No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1893061.0731568774675662No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1857001.0527148222757177No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1089230.6174736488246526No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA688540.3903264748140671No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA668430.3789263159147862No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA635170.360071552862072No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534240.30285534014678483No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA473660.26851314093651935No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA427910.24257792116316768No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326380.1850215744180661No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283620.16078135589328976No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274070.15536755591874313No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264900.15016917416307896No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244290.13848557023895267No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239420.1357248156969587No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235450.13347426219968644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198490.11252200596311643No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185350.10507307071018002No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCC1201.8189894E-1243.0947881
AGTCCGC1152.7775968E-936.786232
CCCGACG25100.036.5125588
GGATCCC1353.45608E-1034.8240741
GTCTCCG1703.6379788E-1233.185291
CCGTTTT22050.033.0473331
GGACCTC2150.032.7994161
CCTGCTG15450.031.6363378
GCCACGG902.5942754E-531.3364122
GGCCCTC4000.030.5581231
GGACCCA3650.029.6243111
GTGCCAA3650.029.6174985
CCGACGC29900.029.5509729
CCTTTTG14650.029.2023031
CTGCTGA22050.029.2009549
AGGACCC2151.8189894E-1228.426161
GGCCTGC2151.8189894E-1228.4160736
GGCCTCC3150.028.3567451
GAACCCG4000.028.2074991
GGTCCTC1503.6945494E-828.2074971
Produced by FastQC (version 0.11.5)