##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248449_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17640105
Sequences flagged as poor quality	0
Sequence length	100
%GC	24
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.237259698851	33.0	31.0	34.0	23.0	34.0
2	30.63131665032606	33.0	31.0	34.0	26.0	34.0
3	31.05432637730898	33.0	31.0	34.0	27.0	34.0
4	34.451175092211756	37.0	35.0	37.0	31.0	37.0
5	34.329742141557546	37.0	35.0	37.0	30.0	37.0
6	34.09138290276617	37.0	35.0	37.0	28.0	37.0
7	34.258470570328235	37.0	35.0	37.0	30.0	37.0
8	34.27525193302421	37.0	35.0	37.0	30.0	37.0
9	35.647630215353026	39.0	35.0	39.0	29.0	39.0
10-11	36.2568809822844	39.0	37.0	39.0	31.0	39.0
12-13	36.365707205257564	39.0	37.0	39.0	31.0	39.0
14-15	37.63953785989369	40.0	38.0	41.0	31.0	41.0
16-17	37.53846127900032	40.0	38.0	41.0	31.0	41.0
18-19	37.14922303467014	40.0	36.0	41.0	31.0	41.0
20-21	36.87810704641497	39.5	35.0	41.0	30.0	41.0
22-23	36.639805913853685	39.0	35.0	41.0	30.0	41.0
24-25	36.50708184560126	39.0	35.0	41.0	29.5	41.0
26-27	36.39826183007414	39.0	35.0	41.0	28.0	41.0
28-29	36.294138073441175	39.0	35.0	41.0	27.0	41.0
30-31	36.20630098290231	39.0	35.0	41.0	27.0	41.0
32-33	36.131061861593224	39.0	35.0	41.0	26.5	41.0
34-35	36.05880271120835	39.0	35.0	41.0	25.5	41.0
36-37	35.95361090537726	39.0	35.0	41.0	25.0	41.0
38-39	35.852661024410004	39.0	35.0	41.0	25.0	41.0
40-41	35.76481715953505	38.5	35.0	41.0	25.0	41.0
42-43	35.6823524293081	38.0	35.0	40.5	25.0	41.0
44-45	35.55310487097441	38.0	35.0	40.0	25.0	41.0
46-47	35.443294668597495	38.0	35.0	40.0	25.0	41.0
48-49	35.32736160017188	38.0	34.5	40.0	25.0	41.0
50-51	35.16997415831709	38.0	34.0	40.0	25.0	41.0
52-53	34.97410432080761	37.5	34.0	40.0	25.0	41.0
54-55	34.79845284367639	37.0	33.5	40.0	25.0	41.0
56-57	34.593351003296185	36.5	33.0	40.0	25.0	41.0
58-59	34.66683741394964	36.5	33.0	40.0	26.0	41.0
60-61	34.49847481066581	36.0	33.0	39.5	26.0	41.0
62-63	34.26850095280045	35.5	33.0	39.0	25.0	41.0
64-65	34.022908508764544	35.0	33.0	39.0	25.0	41.0
66-67	33.7748446225235	35.0	33.0	39.0	25.0	40.5
68-69	33.524888825775136	35.0	32.5	38.5	25.0	40.0
70-71	33.287201975271685	35.0	32.0	37.5	25.0	40.0
72-73	33.18648123126251	35.0	32.0	37.0	25.0	39.5
74-75	33.13956679963073	35.0	33.0	36.5	25.0	39.0
76-77	32.95247553798575	35.0	32.5	36.0	25.0	39.0
78-79	32.72721477564901	35.0	32.0	36.0	25.0	39.0
80-81	32.51234703534928	35.0	32.0	35.5	25.0	37.5
82-83	32.3070595101333	35.0	32.0	35.0	25.0	37.0
84-85	32.11340694967518	35.0	32.0	35.0	25.0	37.0
86-87	31.933099264431817	35.0	32.0	35.0	24.5	36.0
88-89	31.774680479509616	35.0	31.5	35.0	24.0	36.0
90-91	31.617652275879312	35.0	31.0	35.0	24.0	36.0
92-93	31.480642688918234	35.0	31.0	35.0	23.5	36.0
94-95	31.34243407281306	35.0	31.0	35.0	23.0	35.5
96-97	31.211283691338572	35.0	31.0	35.0	23.0	35.0
98-99	31.10007857096089	35.0	31.0	35.0	21.5	35.0
100	30.3833882508069	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31689724520590445
1101	2	0.27023802779224226
1101	3	0.3106408909228797
1101	4	0.24662623925412674
1101	5	0.1658381091008181
1101	6	0.1536635734248577
1101	7	0.1977407861950624
1101	8	0.19021011315276581
1101	9	0.31742980759683803
1101	10-11	0.3343270828872704
1101	12-13	0.3300349039592376
1101	14-15	0.43594074525592674
1101	16-17	0.47579774020243804
1101	18-19	0.47593004693564467
1101	20-21	0.47212311439763255
1101	22-23	0.46307237726501427
1101	24-25	0.4819307236206427
1101	26-27	0.508682700208908
1101	28-29	0.5146264850465201
1101	30-31	0.5159151515030516
1101	32-33	0.5414663205301835
1101	34-35	0.5454851548615736
1101	36-37	0.5388590552203922
1101	38-39	0.5669774927333222
1101	40-41	0.542937872979131
1101	42-43	0.5791294872958872
1101	44-45	0.549359831215817
1101	46-47	0.5428340762249491
1101	48-49	0.5508622836750448
1101	50-51	0.5795978123318477
1101	52-53	0.5546572857957415
1101	54-55	0.5043205120342691
1101	56-57	0.48535021776251597
1101	58-59	0.477062264520832
1101	60-61	0.48061848554241493
1101	62-63	0.4437881113112141
1101	64-65	0.4563176447461501
1101	66-67	0.4449749949545918
1101	68-69	0.4558883500276778
1101	70-71	0.39084209149519467
1101	72-73	0.42002990125879336
1101	74-75	0.4127352391781969
1101	76-77	0.44048042656108066
1101	78-79	0.3671649473259322
1101	80-81	0.38083219876980223
1101	82-83	0.35868662895729386
1101	84-85	0.33037161749285815
1101	86-87	0.33374029179037734
1101	88-89	0.30148376124668275
1101	90-91	0.2940459692742685
1101	92-93	0.2779128027956652
1101	94-95	0.31081912997046146
1101	96-97	0.3133371601949797
1101	98-99	0.3172910225610508
1101	100	0.37149961977515034
1102	1	0.198029372022134
1102	2	0.15462714803722832
1102	3	0.2028919569475427
1102	4	0.14641430638540243
1102	5	0.08799278929028986
1102	6	-0.028056534272785427
1102	7	0.029205303869680677
1102	8	0.07531890086584525
1102	9	0.1654242264345953
1102	10-11	0.21286811763811642
1102	12-13	0.20239532271068583
1102	14-15	0.3294104815052563
1102	16-17	0.3306560910679792
1102	18-19	0.32872204635800983
1102	20-21	0.29848140512850563
1102	22-23	0.3141326483928282
1102	24-25	0.26390499809665613
1102	26-27	0.2714666338093039
1102	28-29	0.28313674360447294
1102	30-31	0.30920594079259445
1102	32-33	0.30467101962512544
1102	34-35	0.318207261890457
1102	36-37	0.3512914099057127
1102	38-39	0.3064666517943735
1102	40-41	0.3227430196229335
1102	42-43	0.33091060691710084
1102	44-45	0.3104130972611685
1102	46-47	0.3383276733706424
1102	48-49	0.32719431379138086
1102	50-51	0.3101093974773903
1102	52-53	0.31010770725988124
1102	54-55	0.28879618738671553
1102	56-57	0.30974632578858774
1102	58-59	0.32911244864624933
1102	60-61	0.308320226537667
1102	62-63	0.27347556695067254
1102	64-65	0.2778192521351741
1102	66-67	0.27795544531549865
1102	68-69	0.2658019489025065
1102	70-71	0.2579083861733622
1102	72-73	0.2635004679744597
1102	74-75	0.27274715274516836
1102	76-77	0.25792766513113463
1102	78-79	0.20878498498263554
1102	80-81	0.21450035847434634
1102	82-83	0.21044498201928974
1102	84-85	0.18385157342146385
1102	86-87	0.1892196572310496
1102	88-89	0.1952834781070898
1102	90-91	0.23000672812475287
1102	92-93	0.16926228143828936
1102	94-95	0.22469521208334697
1102	96-97	0.23546426733648929
1102	98-99	0.21247148858218168
1102	100	0.14084499545266738
1103	1	0.04751644735629412
1103	2	0.02435213175521156
1103	3	0.08488522598899095
1103	4	0.054368249076738095
1103	5	-0.013064618924197191
1103	6	-0.047735514739081
1103	7	-0.01572565373993484
1103	8	0.08035978265238697
1103	9	0.07198635987278834
1103	10-11	0.13486272752329143
1103	12-13	0.12970052403971266
1103	14-15	0.2248590500006813
1103	16-17	0.2724583177727169
1103	18-19	0.20992749372321384
1103	20-21	0.20594540025221875
1103	22-23	0.23803015983881437
1103	24-25	0.22608352672553877
1103	26-27	0.22522108761796034
1103	28-29	0.2337245458317625
1103	30-31	0.1653493260421044
1103	32-33	0.22627482165772506
1103	34-35	0.22351778985105142
1103	36-37	0.2107132138522374
1103	38-39	0.18690240292031035
1103	40-41	0.1971348089654228
1103	42-43	0.2578363764041427
1103	44-45	0.19888169886601048
1103	46-47	0.18509381512359369
1103	48-49	0.20138987248219564
1103	50-51	0.19385962964823733
1103	52-53	0.1895217162296845
1103	54-55	0.19182306514745306
1103	56-57	0.17568172251432657
1103	58-59	0.22113978031241288
1103	60-61	0.2149922408687246
1103	62-63	0.2041591558218272
1103	64-65	0.18716817631970173
1103	66-67	0.16010312203442822
1103	68-69	0.20017869174686354
1103	70-71	0.16986953748679667
1103	72-73	0.18845518371895054
1103	74-75	0.14186098970415628
1103	76-77	0.16187916140789582
1103	78-79	0.13490892222691286
1103	80-81	0.16084177306832714
1103	82-83	0.12720571711329143
1103	84-85	0.1483201659847424
1103	86-87	0.09959929106528875
1103	88-89	0.11404357952258337
1103	90-91	0.16997843766876386
1103	92-93	0.15653273817833124
1103	94-95	0.1203492191909703
1103	96-97	0.1102041970374188
1103	98-99	0.15439858400398876
1103	100	0.09737025568840707
1104	1	0.021340912124216516
1104	2	0.07918120691797981
1104	3	0.12990861891271877
1104	4	0.11894239503884307
1104	5	0.0075390420796424
1104	6	-0.10849026327259281
1104	7	-0.031142024330748086
1104	8	0.031912096842582116
1104	9	0.03243679745169459
1104	10-11	0.12249521030489063
1104	12-13	0.1607157173986664
1104	14-15	0.22284552404711633
1104	16-17	0.2409343483917894
1104	18-19	0.26595031515064704
1104	20-21	0.2504886993047961
1104	22-23	0.23167777644849963
1104	24-25	0.23493805548192626
1104	26-27	0.21836389873392648
1104	28-29	0.18781412679400233
1104	30-31	0.19083180385378284
1104	32-33	0.20793762062218235
1104	34-35	0.2063861763102679
1104	36-37	0.23471467019365377
1104	38-39	0.2222304829254611
1104	40-41	0.262796914846291
1104	42-43	0.26364615761377763
1104	44-45	0.25476824783113017
1104	46-47	0.2932572500761097
1104	48-49	0.30518169088492186
1104	50-51	0.2491209024938854
1104	52-53	0.2647093196307395
1104	54-55	0.27608671255300266
1104	56-57	0.2541981998457885
1104	58-59	0.22982891789600046
1104	60-61	0.24926262969366775
1104	62-63	0.2380384106325053
1104	64-65	0.23039508109539497
1104	66-67	0.24246384822437506
1104	68-69	0.20516152070044313
1104	70-71	0.2196448247096967
1104	72-73	0.23593298998135026
1104	74-75	0.24443760637752376
1104	76-77	0.2585539558414993
1104	78-79	0.2403681658638348
1104	80-81	0.20742154361472842
1104	82-83	0.23461696786937836
1104	84-85	0.16834771434319862
1104	86-87	0.15682656619511448
1104	88-89	0.13320806203791946
1104	90-91	0.17590579864931044
1104	92-93	0.12414919543165226
1104	94-95	0.17076263849408946
1104	96-97	0.21702413132616982
1104	98-99	0.24216945529952127
1104	100	0.27708798378136734
1105	1	0.17307347335991352
1105	2	0.10576003174045923
1105	3	0.17735568437172944
1105	4	0.16941336609028212
1105	5	0.12161779161494479
1105	6	0.08207060832321389
1105	7	0.04868826221139244
1105	8	0.08073422599794355
1105	9	0.12682893389758476
1105	10-11	0.11329516674891948
1105	12-13	0.12737445977105466
1105	14-15	0.20306903508370766
1105	16-17	0.20103987208852914
1105	18-19	0.19800519312161668
1105	20-21	0.20062145058744107
1105	22-23	0.14879359194473096
1105	24-25	0.15158523425451875
1105	26-27	0.17251842715121768
1105	28-29	0.16336606856511793
1105	30-31	0.17817768249765464
1105	32-33	0.2018508736321749
1105	34-35	0.2267219775317031
1105	36-37	0.20466181859615773
1105	38-39	0.18218835528434596
1105	40-41	0.2022067765305664
1105	42-43	0.1935721328804476
1105	44-45	0.24126255705640887
1105	46-47	0.2516206957277092
1105	48-49	0.2093530444972984
1105	50-51	0.19452364805130173
1105	52-53	0.22181679909265029
1105	54-55	0.2120423501162847
1105	56-57	0.18306622525457783
1105	58-59	0.18519029038547075
1105	60-61	0.19317180188351557
1105	62-63	0.181905763986272
1105	64-65	0.1729409684164409
1105	66-67	0.16967081394702177
1105	68-69	0.13752003908284394
1105	70-71	0.12328458450532054
1105	72-73	0.1598238405903274
1105	74-75	0.19647826825813297
1105	76-77	0.1636521064992209
1105	78-79	0.16219148685347307
1105	80-81	0.1894603739327465
1105	82-83	0.15974294670900235
1105	84-85	0.14302187569935398
1105	86-87	0.10623947509593279
1105	88-89	0.14013068808864304
1105	90-91	0.18727012733015158
1105	92-93	0.12770529969035493
1105	94-95	0.12111362390539426
1105	96-97	0.1249029342855934
1105	98-99	0.15058455986362773
1105	100	0.18387256973167254
1106	1	0.03446695257222032
1106	2	-0.0017406465737330734
1106	3	0.08165897911571207
1106	4	0.04073382660843805
1106	5	0.0030553618559281404
1106	6	-0.008677530404462175
1106	7	-0.014999752863872118
1106	8	0.027249520901960977
1106	9	0.07603979161243757
1106	10-11	0.05032857003529756
1106	12-13	0.07394238393565189
1106	14-15	0.10653110222650497
1106	16-17	0.11449814940083058
1106	18-19	0.11134219343318819
1106	20-21	0.11220391617568026
1106	22-23	0.09674113403072226
1106	24-25	0.15055758481828008
1106	26-27	0.1257139590469336
1106	28-29	0.15129619553535179
1106	30-31	0.14950065421294312
1106	32-33	0.16030800688282199
1106	34-35	0.15000766843622415
1106	36-37	0.13795333609230198
1106	38-39	0.11386520292774804
1106	40-41	0.11502284366608961
1106	42-43	0.1375990553061257
1106	44-45	0.11672310471075775
1106	46-47	0.15838123687574068
1106	48-49	0.137149711079104
1106	50-51	0.1186812489083593
1106	52-53	0.1361341870920043
1106	54-55	0.1871100388814071
1106	56-57	0.16657099406400278
1106	58-59	0.1507410406935108
1106	60-61	0.13910696660455102
1106	62-63	0.17690739864871574
1106	64-65	0.11878866960418577
1106	66-67	0.11108817418337935
1106	68-69	0.1205992024104745
1106	70-71	0.15134128324692853
1106	72-73	0.1183683694822264
1106	74-75	0.1217265619655592
1106	76-77	0.13973254239843413
1106	78-79	0.16126187675178016
1106	80-81	0.15560645254121397
1106	82-83	0.13636086689302118
1106	84-85	0.13663549160904154
1106	86-87	0.1091227044291685
1106	88-89	0.1008308691744837
1106	90-91	0.12417902470285114
1106	92-93	0.052098615234360324
1106	94-95	0.07114695808737537
1106	96-97	0.08846541940895492
1106	98-99	0.0587118525837802
1106	100	0.042707286971442215
1107	1	0.01935471310887138
1107	2	0.036545323635870375
1107	3	0.10101441552336254
1107	4	0.07617037423003836
1107	5	0.08480979745453254
1107	6	0.028574221121836274
1107	7	0.012113591303602789
1107	8	0.06679155596491171
1107	9	0.061074710592642134
1107	10-11	0.05592405081527119
1107	12-13	0.06496210523114598
1107	14-15	0.1010013538453407
1107	16-17	0.09729086382268548
1107	18-19	0.1247729347456854
1107	20-21	0.1045941834393318
1107	22-23	0.07274463322864477
1107	24-25	0.08189478928184712
1107	26-27	0.11524687965190594
1107	28-29	0.10373988067594553
1107	30-31	0.14221971015686563
1107	32-33	0.1250758837539152
1107	34-35	0.12524033508005772
1107	36-37	0.13838614654666515
1107	38-39	0.0843163394757056
1107	40-41	0.0935216263888563
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2216	44-45	-0.11042304407340708
2216	46-47	-0.08994405977158237
2216	48-49	-0.10331171843800746
2216	50-51	-0.08900499638199477
2216	52-53	-0.04661274504032775
2216	54-55	-0.13201149966899806
2216	56-57	-0.16051584958469078
2216	58-59	-0.19119508957304276
2216	60-61	-0.19945553771599833
2216	62-63	-0.21538330773528003
2216	64-65	-0.21492389742634543
2216	66-67	-0.21815370601039064
2216	68-69	-0.22049842671252406
2216	70-71	-0.17767344479937464
2216	72-73	-0.209542906903728
2216	74-75	-0.21660412866557976
2216	76-77	-0.19177852682337004
2216	78-79	-0.2135220183854898
2216	80-81	-0.22548951223368618
2216	82-83	-0.22012772605575748
2216	84-85	-0.22205917020757937
2216	86-87	-0.20999718532278067
2216	88-89	-0.18305920441407153
2216	90-91	-0.1689539180904518
2216	92-93	-0.23237562228315412
2216	94-95	-0.1978931217766693
2216	96-97	-0.165161394284425
2216	98-99	-0.21142020772701287
2216	100	-0.1493867807603131
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	20754.0
1	1024.0
2	593.0
3	475.0
4	422.0
5	969.0
6	4528.0
7	11764.0
8	15808.0
9	21401.0
10	31711.0
11	41515.0
12	48115.0
13	53384.0
14	59313.0
15	67826.0
16	77343.0
17	86009.0
18	93610.0
19	101318.0
20	109656.0
21	116775.0
22	125095.0
23	139583.0
24	188069.0
25	242095.0
26	162085.0
27	331865.0
28	206366.0
29	339444.0
30	288116.0
31	347314.0
32	453694.0
33	792705.0
34	986741.0
35	3031867.0
36	2170491.0
37	3112122.0
38	2861357.0
39	889178.0
40	7605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.127434553273407	30.172982470205756	30.236831134845993	23.462751841674844
2	13.02935120875702	32.82564677435334	33.33988124621922	20.80512077067042
3	11.972441035632233	34.76145537548215	32.54893395575218	20.717169633133437
4	10.57799854105029	34.33059455238012	33.75870988377965	21.332697022789944
5	10.65206060361425	33.701465437599126	34.337413590946326	21.309060367840296
6	10.670216308196466	31.770985114642087	35.2338495983863	22.324948978775147
7	13.25244311406001	37.17606150390394	29.982335774900587	19.589159607135464
8	14.637297177478523	32.721324856904204	26.466945133579255	26.17443283203802
9	11.037403051337707	32.1527385396597	29.241630630038447	27.568227778964143
10-11	12.379123833016411	48.953010613165446	27.384065396775696	11.283800157042448
12-13	11.535874766565014	48.7535488532352	28.651318265506394	11.059258114693396
14-15	11.613643673438883	48.17802575485926	28.582390482778784	11.625940088923068
16-17	11.513252735466576	47.96897996542822	28.732320362186886	11.785446936918326
18-19	11.428361702120412	47.70505533363967	29.119619533185254	11.746963431054667
20-21	11.33752403624118	47.560825719787175	29.273622534828743	11.828027709142903
22-23	11.389438254674621	47.58693028162843	29.489766770888547	11.5338646928084
24-25	11.337807138881091	47.290282913652675	29.564452576686612	11.807457370779622
26-27	11.577028170909156	47.28135483332548	29.3844252415797	11.757191754185667
28-29	11.526762481169994	47.404956372067176	29.475532034598956	11.592749112163874
30-31	11.588603298450579	47.403916067361045	29.533737366397222	11.47374326779115
32-33	11.577499453005416	47.384642049809834	29.548130811356188	11.489727685828566
34-35	11.531890539352228	47.489081339387496	29.556581084336305	11.422447036923975
36-37	11.714997625603772	47.376799578447795	29.477553631279157	11.430649164669267
38-39	11.748958946249449	47.408266115525684	29.611433230740026	11.231341707484846
40-41	11.719701208841427	47.70902943910931	29.550611128251337	11.02065822379793
42-43	11.801239679614273	47.67071637908574	29.58559728326292	10.942446658037067
44-45	11.889702012419711	47.56133145819575	29.50868526555937	11.04028126382517
46-47	11.853801969850675	47.50233304059802	29.587499233957683	11.056365755593628
48-49	11.888145911013213	47.44016286692199	29.622348222938477	11.049342999126322
50-51	11.959111820554297	47.39404475140642	29.708028635600098	10.938814792439189
52-53	12.022168466333364	47.32364213474398	29.83200814804592	10.822181250876737
54-55	12.0478177379847	47.266439636028466	29.785757020370053	10.899985605616779
56-57	12.110891775963267	47.27873993353147	29.613881043269547	10.996487247235716
58-59	12.056920943465235	47.134746138331586	29.726994799731987	11.08133811847119
60-61	12.047310087228952	47.13474483412386	29.848233396702827	10.96971168194436
62-63	12.097595861492048	46.9687874809317	29.84838629616527	11.085230361410984
64-65	12.161234430120993	46.94330671416757	29.779690644899294	11.115768210812146
66-67	12.127951645836472	46.83531366793164	29.993901634127624	11.04283305210426
68-69	12.067935331336153	47.01066860680037	29.890589343994417	11.030806717869055
70-71	11.979869472728469	47.191604360202774	29.879956550319704	10.94856961674905
72-73	11.985216802743345	47.19708965666627	29.943887243637235	10.873806296953152
74-75	12.018389540910292	47.3056158532733	29.800837872904275	10.875156732912131
76-77	12.03454941935626	47.43132827091073	29.745848062499174	10.788274247233835
78-79	11.91799020857396	47.57278139537101	29.78724623989681	10.721982156158221
80-81	11.850599427471188	47.70597681527358	29.732589063285243	10.71083469396999
82-83	11.828473145055954	47.7156133136896	29.75289076365565	10.703022777598791
84-85	11.825025890918473	47.85201085021965	29.618191925768116	10.704771333093758
86-87	11.89265384560589	48.01697008940717	29.402664522791227	10.687711542195713
88-89	11.947494138262924	48.0609965756183	29.372404050363972	10.61910523575481
90-91	11.972160311815589	48.0956278820221	29.409588008046395	10.522623798115914
92-93	12.068402064054437	48.139092627847106	29.272683303405493	10.519822004692962
94-95	12.19615226171307	48.03990638143647	29.187336887334602	10.576604469515855
96-97	12.32730552253928	47.83970160642158	29.266518939692165	10.566473931346977
98-99	12.384174382461346	47.82051583713258	29.252781981874687	10.542527798531381
100	12.575331930520578	47.710540555207764	29.204373787610333	10.509753726661321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1489790.0
1	1248934.0
2	602203.5
3	130565.0
4	64796.5
5	63345.5
6	57710.5
7	50233.5
8	46766.5
9	50344.0
10	61879.5
11	82942.5
12	114145.0
13	154177.5
14	201103.5
15	250377.5
16	298076.5
17	341974.0
18	383113.0
19	422865.0
20	461549.0
21	502393.5
22	544368.5
23	586218.0
24	620602.0
25	636395.0
26	638850.5
27	636435.5
28	629318.0
29	613046.5
30	584386.0
31	544855.0
32	499144.5
33	453680.0
34	412412.0
35	375870.0
36	345091.5
37	319283.0
38	295390.0
39	272538.5
40	249674.5
41	226815.5
42	205959.0
43	187896.5
44	172997.0
45	161655.5
46	152172.5
47	142937.5
48	133692.0
49	123182.5
50	109465.5
51	93586.5
52	77518.0
53	61799.5
54	47598.5
55	35712.0
56	26310.5
57	19068.5
58	13477.5
59	9360.0
60	6564.0
61	4781.5
62	3622.0
63	2957.0
64	2515.5
65	2149.0
66	1904.5
67	1673.5
68	1484.5
69	1348.5
70	1199.5
71	1101.0
72	1065.0
73	996.5
74	906.5
75	821.5
76	771.0
77	756.5
78	735.5
79	695.5
80	644.5
81	602.0
82	551.5
83	519.0
84	523.0
85	529.0
86	489.0
87	464.0
88	446.0
89	420.5
90	420.5
91	370.0
92	347.0
93	343.5
94	335.0
95	329.0
96	314.5
97	307.0
98	278.5
99	256.0
100	268.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15956254228645464
2	0.14937552809351193
3	0.15119524515301921
4	0.14775422255139636
5	0.14612157920828703
6	0.12997088169259763
7	0.13118969529943275
8	0.13270896063260393
9	0.13212506388142248
10-11	0.13158651833421628
12-13	0.13520894575174014
14-15	0.1429441604797704
16-17	0.1360876253287608
18-19	0.14355356728318794
20-21	0.1448857588999612
22-23	0.14513235607157668
24-25	0.1458041207804602
26-27	0.14645604433760456
28-29	0.14700025878530767
30-31	0.14525140298201172
32-33	0.14552634465611172
34-35	0.14735739951661284
36-37	0.1482615891458696
38-39	0.14864423992941087
40-41	0.1450926737680983
42-43	0.1425756819474714
44-45	0.14196060624355694
46-47	0.1429498293802673
48-49	0.14443508131045704
50-51	0.14194076509181774
52-53	0.14219870006442703
54-55	0.14509834266859523
56-57	0.14173668467392908
58-59	0.14255867524598068
60-61	0.14413746403436942
62-63	0.13878035306479186
64-65	0.130591626297009
66-67	0.130897746923842
68-69	0.12903834756085636
70-71	0.12745955877246765
72-73	0.13082121676713376
74-75	0.1323801644037833
76-77	0.12952303855334196
78-79	0.12493122915084688
80-81	0.12543576129507167
82-83	0.1262407451656325
84-85	0.12174247262133643
86-87	0.12565684841445104
88-89	0.12490855354885926
90-91	0.13052926839154302
92-93	0.1272639817053243
94-95	0.12966192661551618
96-97	0.13249354241372147
98-99	0.13617549328646286
100	0.13644476606006598
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.7640105E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.9661055624926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24149876944772	53.59185283880501
2	14.448030411421714	19.061605985798963
3	3.042286414183068	6.020633602480121
4	0.6656524691047844	1.7564200417960019
5	0.19738409033901186	0.6510329869829915
6	0.1010619528553921	0.39999980702462673
7	0.060803203493574404	0.28076653781363936
8	0.04388377135116162	0.23158731947448127
9	0.029061651653824516	0.17253755827349182
>10	0.15668161437361844	1.8308983318054366
>50	0.008416197118272725	0.37971125382259285
>100	0.004002887000038339	0.48665268844625376
>500	4.882151377308499E-4	0.218671558439313
>1k	4.129063645111647E-4	0.5927865348681339
>5k	8.601183474748744E-5	0.4303627186982218
>10k+	2.494343207677136E-4	13.894480235470702
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1030152	5.839829184690227	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	244317	1.385008762702943	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	189306	1.0731568774675662	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	185700	1.0527148222757177	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108923	0.6174736488246526	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68854	0.3903264748140671	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66843	0.3789263159147862	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63517	0.360071552862072	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53424	0.30285534014678483	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47366	0.26851314093651935	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42791	0.24257792116316768	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32638	0.1850215744180661	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28362	0.16078135589328976	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27407	0.15536755591874313	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26490	0.15016917416307896	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24429	0.13848557023895267	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23942	0.1357248156969587	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23545	0.13347426219968644	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	19849	0.11252200596311643	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18535	0.10507307071018002	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.53963766088694E-4	0.0	0.0	5.668900496907473E-6	0.0
2	0.0016042988406248149	0.0	0.0	1.1337800993814946E-5	0.0
3	0.002613363129074345	0.0	0.0	3.401340298144484E-5	0.0
4	0.004569133800507423	0.0	0.0	3.968230347835231E-5	0.0
5	0.007607664466849829	0.0	0.0	5.668900496907473E-5	0.0
6	0.013327585068229468	0.0	0.0	5.668900496907473E-5	0.0
7	0.01969376032625656	0.0	0.0	5.668900496907473E-5	0.0
8	0.03744308778207386	0.0	0.0	5.668900496907473E-5	0.0
9	0.10133726528271798	0.0	0.0	5.668900496907473E-5	0.0
10-11	0.23315620853730745	0.0	0.0	5.668900496907473E-5	0.0
12-13	0.3809586167429275	0.0	0.0	5.952345521752847E-5	0.0
14-15	0.546992208946602	0.0	0.0	6.802680596288968E-5	0.0
16-17	0.7291538230639784	0.0	0.0	6.802680596288968E-5	0.0
18-19	0.9054622974182976	0.0	0.0	9.070240795051957E-5	0.0
20-21	1.1371814396796391	0.0	0.0	9.637130844742704E-5	0.0
22-23	1.402531334138884	0.0	0.0	9.637130844742704E-5	0.0
24-25	1.6680512956130364	0.0	0.0	1.0487465919278824E-4	0.0
26-27	1.9123553969775124	0.0	0.0	1.5022586316804803E-4	0.0
28-29	2.1419657082540042	0.0	0.0	1.5872921391340923E-4	0.0
30-31	2.384651338526613	0.0	0.0	1.7290146515567794E-4	0.0
32-33	2.6635414018227213	0.0	0.0	1.8423926614949288E-4	0.0
34-35	2.920934994434557	0.0	0.0	1.9841151739176155E-4	0.0
36-37	3.1227450176742146	0.0	0.0	2.1258376863403025E-4	0.0
38-39	3.275867688996182	0.0	0.0	3.0045172633609606E-4	0.0
40-41	3.4103992011385422	0.0	0.0	3.571407313051708E-4	0.0
42-43	3.5300923662302464	0.0	0.0	4.0249193528043057E-4	0.0
44-45	3.638674486348012	0.0	0.0	4.3367088801342173E-4	0.0
46-47	3.7400769439864447	0.0	0.0	4.6201539049795903E-4	0.0
48-49	3.841998106020344	0.0	0.0	5.272077462123951E-4	0.0
50-51	3.9487576746283537	0.0	0.0	6.094068034175534E-4	0.0
52-53	4.060752472845258	0.0	0.0	6.972747611196192E-4	0.0
54-55	4.173056226139243	0.0	0.0	8.191561218031298E-4	0.0
56-57	4.289597482554667	0.0	0.0	8.673417760268435E-4	0.0
58-59	4.4130888109793	0.0	0.0	9.155274302505569E-4	0.0
60-61	4.545100496850784	0.0	0.0	9.58044183977363E-4	0.0
62-63	4.674949497182698	0.0	0.0	0.0010090642884495302	0.0
64-65	4.802763362236222	0.0	0.0	0.0010884288954062346	0.0
66-67	4.929848773575895	0.0	0.0	0.001162124601866032	0.0
68-69	5.055664351204259	0.0	0.0	0.0012358203083258292	0.0
70-71	5.183464044006541	0.0	0.0	0.001301012664040265	0.0
72-73	5.313352726641933	0.0	0.0	0.0013180193655309876	0.0
74-75	5.446489689262053	0.0	0.0	0.0013435294177670711	0.0
76-77	5.584164606729949	0.0	0.0	0.001442735176462952	0.0
78-79	5.725762403341703	0.0	0.0	0.0016184710918670836	0.0
80-81	5.870551790933217	0.0	0.0	0.0018140481590103914	0.0
82-83	6.020729468447041	0.0	0.0	0.001904750566960911	0.0
84-85	6.177896900273553	2.8344502484537366E-6	0.0	0.0019189228182031797	0.0
86-87	6.339766685062249	5.668900496907473E-6	0.0	0.0019330950694454482	0.0
88	6.464468323742971	5.668900496907473E-6	0.0	0.0019501017709361707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTCCC	120	1.8189894E-12	43.094788	1
AGTCCGC	115	2.7775968E-9	36.78623	2
CCCGACG	2510	0.0	36.512558	8
GGATCCC	135	3.45608E-10	34.824074	1
GTCTCCG	170	3.6379788E-12	33.18529	1
CCGTTTT	2205	0.0	33.047333	1
GGACCTC	215	0.0	32.799416	1
CCTGCTG	1545	0.0	31.636337	8
GCCACGG	90	2.5942754E-5	31.336412	2
GGCCCTC	400	0.0	30.558123	1
GGACCCA	365	0.0	29.624311	1
GTGCCAA	365	0.0	29.617498	5
CCGACGC	2990	0.0	29.550972	9
CCTTTTG	1465	0.0	29.202303	1
CTGCTGA	2205	0.0	29.200954	9
AGGACCC	215	1.8189894E-12	28.42616	1
GGCCTGC	215	1.8189894E-12	28.416073	6
GGCCTCC	315	0.0	28.356745	1
GAACCCG	400	0.0	28.207499	1
GGTCCTC	150	3.6945494E-8	28.207497	1
>>END_MODULE