##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248449_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17640105
Sequences flagged as poor quality	0
Sequence length	100
%GC	24
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.849159117817045	34.0	31.0	34.0	31.0	34.0
2	33.03465070077531	34.0	33.0	34.0	31.0	34.0
3	33.11358724905549	34.0	33.0	34.0	31.0	34.0
4	36.44055072234547	37.0	37.0	37.0	35.0	37.0
5	36.35177500360684	37.0	37.0	37.0	35.0	37.0
6	36.27546837164518	37.0	37.0	37.0	35.0	37.0
7	36.3148468220569	37.0	37.0	37.0	35.0	37.0
8	36.12075540366681	37.0	37.0	37.0	35.0	37.0
9	37.922928576672305	39.0	39.0	39.0	35.0	39.0
10-11	38.107011522890595	39.0	39.0	39.0	36.0	39.0
12-13	38.16296700047987	39.0	39.0	39.0	37.0	39.0
14-15	39.760565144028334	41.0	40.0	41.0	37.0	41.0
16-17	39.76134535480372	41.0	40.0	41.0	37.0	41.0
18-19	39.64354435531988	41.0	39.5	41.0	37.0	41.0
20-21	39.35780747903712	41.0	39.0	41.0	35.5	41.0
22-23	38.98793657974258	41.0	38.5	41.0	35.0	41.0
24-25	38.67667043365105	41.0	37.0	41.0	35.0	41.0
26-27	38.5407985950197	40.0	37.0	41.0	35.0	41.0
28-29	38.44809030331736	40.0	36.0	41.0	35.0	41.0
30-31	38.37668914102269	40.0	36.0	41.0	35.0	41.0
32-33	38.312709278091035	40.0	35.5	41.0	35.0	41.0
34-35	38.251542692064476	40.0	35.0	41.0	35.0	41.0
36-37	38.187137916696074	40.0	35.0	41.0	35.0	41.0
38-39	38.1071792373118	40.0	35.0	41.0	35.0	41.0
40-41	38.02479730137661	40.0	35.0	41.0	35.0	41.0
42-43	37.92351250176799	40.0	35.0	41.0	34.0	41.0
44-45	37.841518602071815	40.0	35.0	41.0	34.0	41.0
46-47	37.76670734102773	40.0	35.0	41.0	34.0	41.0
48-49	37.703434333299036	40.0	35.0	41.0	34.0	41.0
50-51	37.55236933113493	39.5	35.0	41.0	33.5	41.0
52-53	37.338707961205444	39.0	35.0	41.0	33.0	41.0
54-55	37.20359569855168	39.0	35.0	41.0	33.0	41.0
56-57	37.03105004193569	39.0	35.0	41.0	33.0	41.0
58-59	36.829447698865735	38.0	35.0	41.0	33.0	41.0
60-61	36.56979649497551	37.5	35.0	40.5	33.0	41.0
62-63	36.28358892988449	37.0	35.0	40.0	32.0	41.0
64-65	36.02615755405084	36.5	35.0	40.0	32.0	41.0
66-67	35.76636930449111	36.0	35.0	39.0	31.5	41.0
68-69	35.52055937308763	35.5	35.0	39.0	31.0	41.0
70-71	35.328501956195836	35.0	35.0	39.0	31.5	41.0
72-73	35.118510008868995	35.0	35.0	37.5	31.5	40.0
74-75	34.859518154795566	35.0	35.0	37.0	31.0	39.5
76-77	34.61122490484042	35.0	35.0	37.0	31.0	39.0
78-79	34.36936648052832	35.0	35.0	36.0	31.0	39.0
80-81	34.15099479283145	35.0	34.0	36.0	31.0	38.5
82-83	33.95853117087455	35.0	34.0	36.0	31.0	37.0
84-85	33.777497044377	35.0	34.0	35.0	30.5	37.0
86-87	33.62107955139723	35.0	34.0	35.0	30.0	36.5
88-89	33.47897810132083	35.0	34.0	35.0	30.0	36.0
90-91	33.361518709780924	35.0	34.0	35.0	30.0	36.0
92-93	33.23600857251133	35.0	34.0	35.0	29.5	36.0
94-95	33.12201730091742	35.0	34.0	35.0	29.0	36.0
96-97	33.0330215721505	35.0	34.0	35.0	29.0	36.0
98-99	32.93307222377645	35.0	34.0	35.0	29.0	35.0
100	32.37002506504355	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1900036249797381
1101	2	0.19022923933676594
1101	3	0.17380731610415978
1101	4	0.09098272707643673
1101	5	0.10688877132466246
1101	6	-0.03391177230211895
1101	7	0.032120658664325674
1101	8	-0.0439090129439208
1101	9	-0.07959831334018475
1101	10-11	0.018050595195710173
1101	12-13	0.11048900339262957
1101	14-15	0.1772139971741069
1101	16-17	0.1946965489943011
1101	18-19	0.21410114392550383
1101	20-21	0.21706573183580247
1101	22-23	0.20742863751097218
1101	24-25	0.21663932421049026
1101	26-27	0.2093936383815418
1101	28-29	0.21844159115615014
1101	30-31	0.23212701591233298
1101	32-33	0.24038001469735804
1101	34-35	0.2461268900096485
1101	36-37	0.22557396988260336
1101	38-39	0.24046413504317599
1101	40-41	0.22504164405732752
1101	42-43	0.22276870196036924
1101	44-45	0.2548686370539457
1101	46-47	0.2417259377650396
1101	48-49	0.2731638516877837
1101	50-51	0.2474686498243983
1101	52-53	0.19067921143590638
1101	54-55	0.24045943790081736
1101	56-57	0.23394307199987452
1101	58-59	0.23129581799567234
1101	60-61	0.22858124022037174
1101	62-63	0.23058290820575422
1101	64-65	0.22138000858114282
1101	66-67	0.18220383210190505
1101	68-69	0.18738416383521184
1101	70-71	0.1866013504284254
1101	72-73	0.17826738858437352
1101	74-75	0.171531363370228
1101	76-77	0.1979878624366478
1101	78-79	0.22056986211563157
1101	80-81	0.22578494901301838
1101	82-83	0.18086440269326687
1101	84-85	0.16007340639934142
1101	86-87	0.17290230981983967
1101	88-89	0.22189302312932568
1101	90-91	0.1964340771269235
1101	92-93	0.2296861269888879
1101	94-95	0.21453204668970471
1101	96-97	0.22909500392491822
1101	98-99	0.2425165321319369
1101	100	0.19465144122244737
1102	1	0.14393628104689782
1102	2	0.14159187806975382
1102	3	0.14206821890379473
1102	4	0.0690131313060931
1102	5	0.09284577907072844
1102	6	-0.03718633487250855
1102	7	0.00683725090237175
1102	8	-0.08182172526298359
1102	9	-0.07249903720200734
1102	10-11	0.025483814785360437
1102	12-13	0.09599039777692298
1102	14-15	0.15808424203133598
1102	16-17	0.17981603184511386
1102	18-19	0.18350603265017895
1102	20-21	0.16872839507248472
1102	22-23	0.14892690350878013
1102	24-25	0.13395322597155968
1102	26-27	0.14089461995486374
1102	28-29	0.16134567890098594
1102	30-31	0.16486707510249943
1102	32-33	0.17553800177415724
1102	34-35	0.16518523658115214
1102	36-37	0.15140264388506353
1102	38-39	0.20110741023567158
1102	40-41	0.15900288835163678
1102	42-43	0.12211485883648265
1102	44-45	0.11736475721900064
1102	46-47	0.17851553580499058
1102	48-49	0.175872921466123
1102	50-51	0.15386253089895519
1102	52-53	0.1108910390957476
1102	54-55	0.1595864359447603
1102	56-57	0.14875969226120844
1102	58-59	0.15430358196619665
1102	60-61	0.155660853584898
1102	62-63	0.16263131786847396
1102	64-65	0.11165364196845218
1102	66-67	0.11627671974361675
1102	68-69	0.11079575030390032
1102	70-71	0.10604910579900206
1102	72-73	0.07470119144481657
1102	74-75	0.09666349402571939
1102	76-77	0.09923427130821949
1102	78-79	0.09553224190789678
1102	80-81	0.09734477720073187
1102	82-83	0.08595453193674274
1102	84-85	0.06527879374325352
1102	86-87	0.10498354473581628
1102	88-89	0.1341122239626742
1102	90-91	0.11749169727105624
1102	92-93	0.12765129578043144
1102	94-95	0.09350778690586026
1102	96-97	0.10824655913874182
1102	98-99	0.1551996592292184
1102	100	0.12893806066468727
1103	1	0.15590047186261557
1103	2	0.14328126806184116
1103	3	0.142702066704949
1103	4	0.07938676981827797
1103	5	0.0809359395574063
1103	6	-0.0028919442643342563
1103	7	0.01870212694309714
1103	8	-0.027614418318748335
1103	9	-0.1241745560984242
1103	10-11	-0.012606917050796085
1103	12-13	0.07477421308137622
1103	14-15	0.15191865879653932
1103	16-17	0.18184561428194712
1103	18-19	0.17407990222225322
1103	20-21	0.15936968003554597
1103	22-23	0.11751565971655964
1103	24-25	0.12586858275863477
1103	26-27	0.11758129026572561
1103	28-29	0.12415938367047374
1103	30-31	0.10055739368177541
1103	32-33	0.11163153341513521
1103	34-35	0.13887345424875974
1103	36-37	0.14812520533134688
1103	38-39	0.14348518400994692
1103	40-41	0.13122143741445313
1103	42-43	0.1616244447629711
1103	44-45	0.14436334394662964
1103	46-47	0.1403437810339625
1103	48-49	0.13267440128051078
1103	50-51	0.13265045530268083
1103	52-53	0.09807202385408686
1103	54-55	0.11019709696051194
1103	56-57	0.09368173047064232
1103	58-59	0.10043799876025616
1103	60-61	0.11500812431865626
1103	62-63	0.09484442083574862
1103	64-65	0.08887387862832696
1103	66-67	0.08298509267785192
1103	68-69	0.08288542158461354
1103	70-71	0.08711180236270621
1103	72-73	0.10587210294634275
1103	74-75	0.08839916043995544
1103	76-77	0.07324882186730974
1103	78-79	0.08734170282041731
1103	80-81	0.06762794242682446
1103	82-83	0.04304367659482722
1103	84-85	0.05783207472725138
1103	86-87	0.06660537794235921
1103	88-89	0.09278154621810586
1103	90-91	0.07298222244029517
1103	92-93	0.059161490761617586
1103	94-95	0.02815372384637982
1103	96-97	0.023826491844552322
1103	98-99	0.04695174280322334
1103	100	-0.002632072147136455
1104	1	-0.28613157630758934
1104	2	-0.25457349755522074
1104	3	-0.21074410647971575
1104	4	-0.23244542241662458
1104	5	-0.1072433702922595
1104	6	-0.05482630589413162
1104	7	0.004971600075108995
1104	8	-0.10460149452890022
1104	9	-0.004842853189188645
1104	10-11	0.06544573817908628
1104	12-13	0.0971101690198779
1104	14-15	0.14606599822691635
1104	16-17	0.15580211760494223
1104	18-19	0.16398511916445102
1104	20-21	0.15103386534755003
1104	22-23	0.1359590133030153
1104	24-25	0.12902263618811816
1104	26-27	0.12500998807497155
1104	28-29	0.13112363340208333
1104	30-31	0.14337404962386557
1104	32-33	0.14733010014643355
1104	34-35	0.171907686480111
1104	36-37	0.12679728642032018
1104	38-39	0.14076216921544216
1104	40-41	0.1421185476961
1104	42-43	0.14982788535829172
1104	44-45	0.1494624926011454
1104	46-47	0.16403511945556204
1104	48-49	0.17330795670004306
1104	50-51	0.1650969140550984
1104	52-53	0.12024545692506905
1104	54-55	0.15968458582779022
1104	56-57	0.16086815840772317
1104	58-59	0.12081257237043985
1104	60-61	0.1468073590042991
1104	62-63	0.10440328811132105
1104	64-65	0.12792635833236687
1104	66-67	0.10487189726489987
1104	68-69	0.09267957648356173
1104	70-71	0.10733472831189772
1104	72-73	0.11298966990909065
1104	74-75	0.12596084569018018
1104	76-77	0.09787388656202722
1104	78-79	0.10071335508873602
1104	80-81	0.0909476017010249
1104	82-83	0.10449931897524323
1104	84-85	0.08606861358115481
1104	86-87	0.060463888050790615
1104	88-89	0.045178604274951795
1104	90-91	0.05106157906679698
1104	92-93	0.07630885292196155
1104	94-95	0.06918568506545597
1104	96-97	0.0731363956336466
1104	98-99	0.06382546322787874
1104	100	0.006103192836469873
1105	1	0.11713596879452837
1105	2	0.11264842777180917
1105	3	0.10321700280238133
1105	4	0.04576056840089393
1105	5	0.0733535710917792
1105	6	0.018997386001821326
1105	7	0.022680161290388412
1105	8	-0.09146951057995523
1105	9	-0.033562326366116224
1105	10-11	0.046700585694907204
1105	12-13	0.08655790057898827
1105	14-15	0.12614617268899764
1105	16-17	0.13988525081050085
1105	18-19	0.13360341148116106
1105	20-21	0.13232185283201403
1105	22-23	0.11671294078401928
1105	24-25	0.11122411487929895
1105	26-27	0.08855592048325178
1105	28-29	0.10890315597079336
1105	30-31	0.1304136115460821
1105	32-33	0.1283480508209962
1105	34-35	0.12632118746989818
1105	36-37	0.12247622957552551
1105	38-39	0.10925353926339909
1105	40-41	0.08739294435657996
1105	42-43	0.0928344680946438
1105	44-45	0.1176437994333952
1105	46-47	0.141388428135528
1105	48-49	0.10528447816029285
1105	50-51	0.09291826141481607
1105	52-53	-0.12117893646021116
1105	54-55	-0.06824745069457094
1105	56-57	-0.018278643928987037
1105	58-59	0.02277551576724335
1105	60-61	0.04615067703349496
1105	62-63	-0.007774934073466966
1105	64-65	-0.00979530880643864
1105	66-67	5.610462986282982E-4
1105	68-69	0.0049487285628018185
1105	70-71	-0.0136292597476384
1105	72-73	-0.05416863732894228
1105	74-75	-0.026584081187685626
1105	76-77	-0.028680177711947863
1105	78-79	-0.014681716280954049
1105	80-81	-0.014159296731044435
1105	82-83	0.0029917677140360865
1105	84-85	-0.032314330172944494
1105	86-87	-0.02824803684238475
1105	88-89	-0.029640117816683187
1105	90-91	-0.008644743950071643
1105	92-93	-0.018969855099051358
1105	94-95	-0.014150374769563712
1105	96-97	-0.012861065869934407
1105	98-99	-0.05805649158450166
1105	100	-0.06586342729676886
1106	1	0.09020127083125118
1106	2	0.09198271886781129
1106	3	0.08512710485462094
1106	4	0.04995156324958572
1106	5	0.042955884593766314
1106	6	0.014751099554807467
1106	7	-8.156433454260537E-4
1106	8	-0.05232064718560281
1106	9	-0.0543872404806649
1106	10-11	0.03763763078289628
1106	12-13	0.09318436465257918
1106	14-15	0.06883989029125814
1106	16-17	0.08355392958700492
1106	18-19	0.0635679403164815
1106	20-21	0.08449759702774884
1106	22-23	0.08400870476543787
1106	24-25	0.0929205639236983
1106	26-27	0.11444784605903635
1106	28-29	0.09503321367499495
1106	30-31	0.11010664093323186
1106	32-33	0.08844330981497706
1106	34-35	0.09027949610143793
1106	36-37	0.10204940850297106
1106	38-39	0.10854984799758682
1106	40-41	0.09290920712624029
1106	42-43	0.08345713910478736
1106	44-45	0.07557930199813967
1106	46-47	0.09153025540929605
1106	48-49	0.08272132664426124
1106	50-51	0.09871479544549544
1106	52-53	0.036012896935972094
1106	54-55	0.07299138419725182
1106	56-57	0.08980125449159004
1106	58-59	0.08182356271943547
1106	60-61	0.1254401028082981
1106	62-63	0.10005142315099391
1106	64-65	0.11151489273996162
1106	66-67	0.1108732842020217
1106	68-69	0.09661018896093054
1106	70-71	0.07508919561153249
1106	72-73	0.06560622540185079
1106	74-75	0.08555090192215431
1106	76-77	0.10169840041056943
1106	78-79	0.09875241167210191
1106	80-81	0.06595273402081148
1106	82-83	0.0785165873050957
1106	84-85	0.07302388572939122
1106	86-87	0.05551422140842277
1106	88-89	0.05617853446979382
1106	90-91	0.061002114002413066
1106	92-93	0.06512971180632832
1106	94-95	0.04966910596142071
1106	96-97	0.057591850800271516
1106	98-99	0.07725639088913283
1106	100	0.05129863705761295
1107	1	0.030501044188000037
1107	2	0.039779630235308616
1107	3	0.0547745893839533
1107	4	0.02511616924846294
1107	5	0.0048793236719646416
1107	6	0.0017911220443949105
1107	7	-0.009128093917510682
1107	8	-0.13043597533706475
1107	9	-0.11127309954934361
1107	10-11	-0.04463857605700383
1107	12-13	0.0015591703533388568
1107	14-15	0.0031976758791927296
1107	16-17	0.012802731239325738
1107	18-19	0.03816979979568913
1107	20-21	-0.0026483946508548684
1107	22-23	0.019279457159576907
1107	24-25	0.04028972842461087
1107	26-27	0.07302820561408652
1107	28-29	0.02908913604647978
1107	30-31	0.03653038547206933
1107	32-33	0.011981575621987872
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2216	18-19	-0.3154013626622856
2216	20-21	-0.24914108238443333
2216	22-23	-0.20199124437650795
2216	24-25	-0.1622350311082883
2216	26-27	-0.2019109865857729
2216	28-29	-0.17928714992822847
2216	30-31	-0.2017887703118859
2216	32-33	-0.19587863167495811
2216	34-35	-0.18089913567439453
2216	36-37	-0.1958701201862496
2216	38-39	-0.21185984785419265
2216	40-41	-0.19288499073592646
2216	42-43	-0.18638352624088128
2216	44-45	-0.20584724217433603
2216	46-47	-0.21161052199165908
2216	48-49	-0.2091151880456863
2216	50-51	-0.22653805148318185
2216	52-53	-0.2130771640412945
2216	54-55	-0.230719311111919
2216	56-57	-0.23491395344488097
2216	58-59	-0.28655467336086105
2216	60-61	-0.2501633423439955
2216	62-63	-0.2319859445468353
2216	64-65	-0.2567750991110884
2216	66-67	-0.24940968660946083
2216	68-69	-0.22861165251395477
2216	70-71	-0.22606154238194875
2216	72-73	-0.21881798180659473
2216	74-75	-0.19772788299221133
2216	76-77	-0.19670328118154146
2216	78-79	-0.21305227209244748
2216	80-81	-0.19335954892844143
2216	82-83	-0.21958307596602822
2216	84-85	-0.2097284800651522
2216	86-87	-0.21693999390500807
2216	88-89	-0.23011721648678218
2216	90-91	-0.23998250754720374
2216	92-93	-0.23971014904634558
2216	94-95	-0.23026730980195254
2216	96-97	-0.274081547990896
2216	98-99	-0.2725444610678167
2216	100	-0.1819223956749383
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	11.0
7	110.0
8	436.0
9	1014.0
10	1651.0
11	2744.0
12	4157.0
13	5816.0
14	7376.0
15	9097.0
16	11549.0
17	14496.0
18	18309.0
19	23238.0
20	29728.0
21	38343.0
22	47353.0
23	56431.0
24	68018.0
25	81515.0
26	98277.0
27	116930.0
28	141900.0
29	178377.0
30	210783.0
31	261641.0
32	336823.0
33	476205.0
34	679202.0
35	3045115.0
36	1601817.0
37	3571877.0
38	4675854.0
39	1802155.0
40	21756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.26450569083954	23.4026003506608	33.61352408210629	24.719369876393372
2	13.639591319913233	25.953429272821193	37.42739799210975	22.97958141515582
3	11.359783955738544	22.05308601334154	41.72266931306299	24.86446071785692
4	11.565858097888714	20.759413169085473	45.142297420478535	22.532431312547278
5	9.954707672141037	20.62333718588848	47.243557742544425	22.178397399426057
6	8.970688299028764	17.288649234827076	44.77934897683839	28.961313489305766
7	11.110755860013304	18.663466005446114	42.59311381649939	27.63266431804119
8	4.331697572094951	19.336755648563315	45.92840008605391	30.403146693287823
9	7.511741001541658	16.895182880147257	46.46671320833974	29.12636290997134
10-11	11.660140251220314	32.04795322207698	43.97958018521761	12.312326341485097
12-13	10.314530943221497	32.73510239479364	44.367856199848035	12.582510462136822
14-15	10.397790963222564	31.674656647111853	44.60703034945397	13.32052204021161
16-17	10.870042761749616	31.674805714990605	44.9596413028887	12.495510220371083
18-19	10.95167571376458	30.957877494423926	44.72395131658872	13.36649547522278
20-21	10.230085265780499	30.83650329035601	45.42185907955973	13.511552364303764
22-23	10.296620677384924	30.839210358836915	45.908658564836564	12.955510398941591
24-25	10.211482405746152	30.537783653548807	45.940785085120794	13.309948855584244
26-27	10.77514851418074	30.244071514929256	45.55493476328649	13.42584520760352
28-29	10.46172150891663	30.9308912224537	45.880878102989875	12.726509165639799
30-31	10.467426570911192	30.700332729157164	45.94196989210006	12.890270807831586
32-33	10.495703849703958	30.686867600021	46.07902233966407	12.73840621061097
34-35	10.523907195869496	30.789142597695456	46.03279853743453	12.654151669000512
36-37	10.573697771252185	30.549735118296823	46.0138889282566	12.862678182194395
38-39	10.615586517516373	30.761020809328837	46.006445605636074	12.61694706751871
40-41	10.656013134622025	31.09727035790309	45.88864919191343	12.358067315561454
42-43	10.753445366494285	31.10471085928091	45.81375920987316	12.328084564351645
44-45	10.82717786385864	30.916034991604025	45.7368658043879	12.51992134014943
46-47	10.842515542721154	30.99213212631001	45.74950014427364	12.415852186695192
48-49	10.762799855227033	30.815743632232902	45.89701884636443	12.524437666175633
50-51	10.673169639606597	30.740193766772617	46.067234171218566	12.519402422402218
52-53	10.76852229154627	30.721976167043643	46.00302778349049	12.506473757919606
54-55	10.686388455498424	30.700105022782903	45.998351925755934	12.615154595962741
56-57	10.703410314491878	30.64989976799417	46.04965942886634	12.59703048864761
58-59	10.752826908297383	30.71772379538335	46.06108401963693	12.468365276682338
60-61	10.746318773588575	30.667967318099755	46.10762370937822	12.478090198933453
62-63	10.665834357773479	30.61242031088835	46.105581047850244	12.616164283487924
64-65	10.807019167247054	30.581565601348377	45.93889340923201	12.672521822172564
66-67	10.731430732502359	30.51697493895554	46.19321830259399	12.558376025948114
68-69	10.779088207243301	30.618584340975996	46.23130119099875	12.371026260781957
70-71	10.790663022049705	30.76524198884502	46.191477334258664	12.25261765484661
72-73	10.836036414939443	30.551554516313782	46.35315805478819	12.25925101395858
74-75	10.818522624001263	30.435395690004135	46.317018006731736	12.429063679262867
76-77	10.899557976354723	30.449236111339474	46.26832981828849	12.38287609401731
78-79	10.962612552058047	30.566907612697484	46.33055442836632	12.139925406878145
80-81	10.920390636375059	30.506534684048642	46.451854912709855	12.12121976686644
82-83	10.987016057497765	30.34977920692522	46.485592816011064	12.17761191956595
84-85	10.975648476167965	30.293471748697847	46.4241302118022	12.306749563331985
86-87	10.990405816608336	30.43656312561055	46.298917039965936	12.274114017815178
88-89	11.030553136391006	30.75633014933419	46.06435583079824	12.148760883476562
90-91	11.045476154887208	30.81006113228742	46.04520104726287	12.099261665562503
92-93	11.023346097681706	30.74428417778863	46.038926547461564	12.1934431770681
94-95	11.087632051285578	30.75502905615153	45.954412915326756	12.202925977236138
96-97	11.095361262722221	30.950414594774678	45.94180255588046	12.012421586622644
98-99	11.03057797195333	31.20755553591425	45.86921151487194	11.892654977260477
100	11.119937817453861	31.347163771750143	45.7045739435402	11.828324467255797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238005.0
1	595374.0
2	1045778.0
3	819045.0
4	305263.5
5	76646.5
6	34413.0
7	25622.5
8	26186.5
9	31890.0
10	43178.0
11	61709.5
12	88688.5
13	125161.0
14	171429.0
15	223223.5
16	276604.0
17	329283.0
18	376472.5
19	416976.0
20	453867.5
21	490550.0
22	533491.5
23	579904.0
24	614866.0
25	631860.5
26	637838.5
27	641070.0
28	639980.0
29	630257.0
30	606072.0
31	566371.5
32	517733.0
33	469033.5
34	426371.5
35	388025.5
36	355021.5
37	327310.5
38	300541.0
39	274157.5
40	247463.0
41	220045.5
42	193672.0
43	169551.5
44	148816.5
45	132540.5
46	122141.5
47	115765.5
48	110614.5
49	106719.5
50	104982.5
51	105173.5
52	105870.5
53	104811.0
54	98558.0
55	86039.0
56	68693.0
57	49613.0
58	32037.0
59	18276.5
60	9619.5
61	4962.0
62	2631.5
63	1478.5
64	894.5
65	599.0
66	453.0
67	365.0
68	282.0
69	219.5
70	185.0
71	158.5
72	130.5
73	102.5
74	82.0
75	56.5
76	46.5
77	38.5
78	30.5
79	24.5
80	17.0
81	15.5
82	14.5
83	10.0
84	6.5
85	6.0
86	5.5
87	5.0
88	3.5
89	1.0
90	1.0
91	2.0
92	1.0
93	1.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007976142999148814
2	5.498833482000249E-4
3	9.070240795051957E-5
4	0.004240337571686789
5	0.013945495222392383
6	0.007636008969334366
7	0.0
8	0.0
9	0.0
10-11	0.011570225914188152
12-13	0.005116182698458994
14-15	0.001130945649133041
16-17	2.324249203732064E-4
18-19	5.668900496907474E-5
20-21	8.191561218031298E-4
22-23	9.35368581989733E-5
24-25	3.174584278268185E-4
26-27	0.002604859778328984
28-29	0.0016071332908732684
30-31	0.0021683544400671086
32-33	0.0020351352783897826
34-35	0.0023582626067135087
36-37	8.786795770206583E-4
38-39	0.0010204020894433452
40-41	4.818565422371353E-5
42-43	2.8344502484537366E-6
44-45	5.782278506845622E-4
46-47	8.503350745361209E-5
48-49	4.053263855288843E-4
50-51	0.032363752936844764
52-53	0.03635749333691608
54-55	6.972747611196192E-4
56-57	1.7573591540413167E-4
58-59	4.1949863677115303E-4
60-61	0.004189317467214623
62-63	0.013732911453758354
64-65	0.017967580124948236
66-67	0.019104194674578183
68-69	0.01995736419936276
70-71	0.01777200305780493
72-73	0.015155805478482129
74-75	0.01641430138879559
76-77	0.014665445585499632
78-79	0.015997637202272888
80-81	0.01605999510773887
82-83	0.014455696267114055
84-85	0.014535060874070763
86-87	0.01323688266027895
88-89	0.011417165600771651
90-91	0.00880663692194576
92-93	0.00937636142188496
94-95	0.01093814350878297
96-97	0.006431367613741528
98-99	0.00873861011598287
100	0.009722164352196316
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.7640105E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.888070236236445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20469488733845	47.78049579754807
2	16.78657672223428	20.777776784232135
3	4.08390733024422	7.582354310773057
4	1.0589441798993278	2.6214404712745365
5	0.3032932622783516	0.9385117359029955
6	0.14464441859346425	0.5371058362315141
7	0.08592090184977508	0.3722235165907761
8	0.05285501110448181	0.26168757116569824
9	0.041519901571768804	0.2312627926207727
>10	0.20796899809039857	2.4006927989855993
>50	0.01651489961772904	0.7145164043848861
>100	0.00916084362692404	1.034913020103067
>500	0.0012361918520414827	0.5395061688449801
>1k	0.0013308467674787053	1.8377261942776117
>5k	4.8637859842478004E-4	2.264353036667369
>10k+	9.452263327877798E-4	10.105433560396811
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38576	0.2186835055687027	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27403	0.15534488031675547	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26224	0.14866124663090158	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25951	0.14711363679524583	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25545	0.1448120631935014	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25482	0.14445492246219624	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25315	0.14350821607921269	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24849	0.14086650844765378	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23207	0.13155817383173174	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23094	0.13091758807558118	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22723	0.1288144259912285	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22681	0.1285763321703584	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22662	0.12846862306091714	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22357	0.12673960840936035	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22218	0.12595163124029024	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21285	0.12066254707667556	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21176	0.12004463692251266	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21125	0.11975552299717036	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20847	0.1181795686590301	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20757	0.11766936761430842	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20689	0.1172838823805187	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20593	0.11673966793281561	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20576	0.11664329662436816	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20317	0.11517505139566912	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20208	0.11455714124150622	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20090	0.11388821098287114	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20081	0.11383719087839897	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19984	0.11328730753019894	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19816	0.11233493224671849	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19667	0.11149026607267928	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19356	0.10972723801814105	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19097	0.10825899278944201	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19094	0.1082419860879513	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18901	0.10714788829204816	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18857	0.10689845667018422	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18696	0.10598576369018212	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18479	0.1047556122823532	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18462	0.10465924097390576	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18398	0.10429643134210369	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18282	0.10363883888446243	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18233	0.10336106276011396	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18171	0.10300959092930569	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18090	0.1025504099890562	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17927	0.10162637920806027	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17900	0.10147331889464375	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17805	0.10093477334743754	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17723	0.10046992350669114	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.179101541629146E-4	0.0	0.0	0.0	0.0
2	0.0013832117212454235	0.0	0.0	1.1337800993814946E-5	0.0
3	0.0023299181042289716	0.0	0.0	1.700670149072242E-5	0.0
4	0.004070270556779566	0.0	0.0	2.8344502484537366E-5	0.0
5	0.007103132322625064	0.0	0.0	2.8344502484537366E-5	0.0
6	0.012845728525992334	0.0	0.0	2.8344502484537366E-5	0.0
7	0.018837756351223534	0.0	0.0	2.8344502484537366E-5	0.0
8	0.03721633176219756	0.0	0.0	2.8344502484537366E-5	0.0
9	0.10469325437688722	0.0	0.0	2.8344502484537366E-5	0.0
10-11	0.24214425027515427	0.0	0.0	2.8344502484537366E-5	0.0
12-13	0.3911569687368641	0.0	0.0	2.8344502484537366E-5	0.0
14-15	0.5549740208462478	0.0	0.0	3.968230347835231E-5	0.0
16-17	0.7293947513350969	0.0	0.0	5.1020104472167255E-5	0.0
18-19	0.8954085023870323	0.0	0.0	7.369570645979716E-5	0.0
20-21	1.1135392901572865	0.0	0.0	9.070240795051957E-5	0.0
22-23	1.3659782637348248	0.0	0.0	9.35368581989733E-5	0.0
24-25	1.6255770586399572	0.0	0.0	9.920575869588077E-5	0.0
26-27	1.8669758484997678	0.0	0.0	1.0770910944124199E-4	0.0
28-29	2.0965152985200484	0.0	0.0	1.2471581093196442E-4	0.0
30-31	2.341641390456576	0.0	0.0	1.2471581093196442E-4	0.0
32-33	2.6278698454459315	0.0	0.0	1.3605361192577936E-4	0.0
34-35	2.9100251954282585	0.0	0.0	1.3605361192577936E-4	0.0
36-37	3.1212200834405466	0.0	0.0	1.3605361192577936E-4	0.0
38-39	3.2729822186432562	0.0	0.0	1.6156366416186297E-4	0.0
40-41	3.408950797061582	0.0	0.0	1.8990816664640035E-4	0.0
42-43	3.529139990946766	0.0	0.0	2.6076942285774377E-4	0.0
44-45	3.636262369186578	0.0	0.0	3.0328617658454977E-4	0.0
46-47	3.7357288973053167	0.0	0.0	3.3163067906908717E-4	0.0
48-49	3.8349658349539304	0.0	0.0	3.741474327958932E-4	0.0
50-51	3.939231087343301	0.0	0.0	4.5351203975259785E-4	0.0
52-53	4.050185642319022	0.0	0.0	5.527177984484787E-4	0.0
54-55	4.156568229043988	0.0	0.0	6.207446044113683E-4	0.0
56-57	4.262962153569948	0.0	0.0	6.519235571443594E-4	0.0
58-59	4.368270483650749	0.0	0.0	6.972747611196192E-4	0.0
60-61	4.475715989218886	0.0	0.0	7.567982163371476E-4	0.0
62-63	4.585446061687275	0.0	0.0	7.879771690701388E-4	0.0
64-65	4.69758541686685	0.0	0.0	8.475006242876673E-4	0.0
66-67	4.8101017539294695	0.0	0.0	9.58044183977363E-4	0.0
68-69	4.923817063447185	0.0	0.0	0.0010175676391948915	0.0
70-71	5.042566356606154	0.0	0.0	0.0010487465919278827	0.0
72-73	5.163404639598234	0.0	0.0	0.0010940977959031423	0.0
74-75	5.288948110002746	0.0	0.0	0.001142283450126856	0.0
76-77	5.419312980279879	0.0	0.0	0.0012273169575804678	0.0
78-79	5.55118294363894	0.0	0.0	0.0014540729774567668	0.0
80-81	5.68797067817907	0.0	0.0	0.0017715314052835854	0.0
82-83	5.83063989698474	0.0	0.0	0.0018764060644763736	0.0
84-85	5.979995583926513	0.0	0.0	0.001916088367954726	0.0
86-87	6.133977093673762	0.0	0.0	0.0019387639699423558	0.0
88	6.253437833845093	0.0	0.0	0.0019444328704392633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAGGC	775	0.0	62.469795	1
GCTTTCT	3200	0.0	60.958275	1
GCTTTTC	3690	0.0	52.99093	1
GCCTTTT	4490	0.0	52.44764	1
GATTTCT	4815	0.0	43.343224	1
CTAGCTT	765	0.0	42.39574	1
AGGCATA	1120	0.0	42.384266	4
GCCCTAC	5515	0.0	41.505856	7
GGCATAT	1200	0.0	40.740242	5
GGCTGCC	105	1.1405064E-9	40.28912	1
TGCTCTA	2310	0.0	39.47867	6
GGCTCTA	875	0.0	39.21819	6
GTTCTAC	1770	0.0	39.036423	7
GCCGCGT	230	0.0	38.832672	5
GGTCCCG	135	9.094947E-12	38.296604	3
GACTTTT	5195	0.0	37.186974	1
TGCCCTA	3350	0.0	36.34358	6
CCGGAAG	760	0.0	35.870743	7
TGGTCCC	315	0.0	35.810013	2
AGGTCCC	305	0.0	35.443108	2
>>END_MODULE