FastQCFastQC Report
Thu 26 May 2016
SRR1248448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370171
Sequences flagged as poor quality0
Sequence length100
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8132906.082869097186565No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1929341.4430181932602058No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1541541.1529695469115542No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1485101.1107561750706105No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA871300.6516745372964938No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA604720.452290400773483No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA544720.40741438535079316No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529780.3962402575105434No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450640.3370487931680156No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430580.32204524534502965No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359240.2686876630074514No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA285900.2138342134891169No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269390.20148582991197347No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244060.1825406720676946No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231350.1730344361339881No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224490.16790361170399393No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216480.16191266364506482No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212620.15902563998620511No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188690.14112758916845566No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164890.12332676971745536No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162950.12187577855212173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150310.11242189796974175No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147010.10995371712149381No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142190.10634867721587106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC1151.8189894E-1244.962693
CCATGGG857.928975E-944.242522
GTAAGCC857.930794E-944.241363
GTCCGCG450.001012911741.789771
GTCCCGT1800.041.789771
GCCGTAC1305.456968E-1239.775732
CCCGACG20250.038.527918
CCGTTTG5750.038.4284171
GAGTCCC1500.037.610791
GTCACGC757.4411873E-637.6061442
AGTGGCC1003.3274773E-837.605163
CTGCCAA10300.037.4209255
CATTGCC3400.037.3286483
TTGCGCC1401.2732926E-1136.933643
ATTGCCG1152.7775968E-936.7862784
CCGTTGT6800.036.6428681
GCCATGG906.182927E-736.5660481
CGAGCCT651.4134646E-436.157454
TGGCGCC1055.1129973E-835.8144343
CCTGCTG9250.035.5671168
Produced by FastQC (version 0.11.5)