##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248448_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13370171 Sequences flagged as poor quality 0 Sequence length 100 %GC 22 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18087128429397 33.0 31.0 34.0 23.0 34.0 2 30.593534293615242 33.0 31.0 34.0 26.0 34.0 3 30.969544892133392 33.0 31.0 34.0 27.0 34.0 4 34.42298067840718 37.0 35.0 37.0 31.0 37.0 5 34.30004021638916 37.0 35.0 37.0 30.0 37.0 6 34.10162285882507 37.0 35.0 37.0 28.0 37.0 7 34.28580068272874 37.0 35.0 37.0 30.0 37.0 8 34.290427175538746 37.0 35.0 37.0 30.0 37.0 9 35.68560349751697 39.0 37.0 39.0 29.0 39.0 10-11 36.319487387259294 39.0 37.0 39.0 31.0 39.0 12-13 36.41704354417008 39.0 37.0 39.0 31.0 39.0 14-15 37.711500324117026 40.0 38.0 41.0 31.0 41.0 16-17 37.60559778928781 40.0 38.0 41.0 31.0 41.0 18-19 37.18377921269668 40.0 36.0 41.0 31.0 41.0 20-21 36.88550621379487 39.5 35.0 41.0 30.0 41.0 22-23 36.63360319026586 39.0 35.0 41.0 30.0 41.0 24-25 36.483080994252056 39.0 35.0 41.0 29.5 41.0 26-27 36.368861363104486 39.0 35.0 41.0 28.0 41.0 28-29 36.25743058933203 39.0 35.0 41.0 27.0 41.0 30-31 36.170837829972406 39.0 35.0 41.0 26.5 41.0 32-33 36.09436995981577 39.0 35.0 41.0 25.5 41.0 34-35 36.02033362176146 39.0 35.0 41.0 25.0 41.0 36-37 35.911719790270446 39.0 35.0 41.0 25.0 41.0 38-39 35.808217224746045 39.0 35.0 41.0 25.0 41.0 40-41 35.71444280705161 38.5 35.0 41.0 25.0 41.0 42-43 35.62358196465849 38.0 35.0 40.5 25.0 41.0 44-45 35.49245144284243 38.0 35.0 40.0 25.0 41.0 46-47 35.38257079883272 38.0 35.0 40.0 25.0 41.0 48-49 35.25424383128683 38.0 34.0 40.0 25.0 41.0 50-51 35.08898446399826 37.5 34.0 40.0 25.0 41.0 52-53 34.8902582846547 37.0 34.0 40.0 25.0 41.0 54-55 34.71345310392814 37.0 33.0 40.0 25.0 41.0 56-57 34.506617604217624 36.0 33.0 40.0 25.0 41.0 58-59 34.57705275422431 36.0 33.0 40.0 25.5 41.0 60-61 34.415986115660004 36.0 33.0 39.5 25.5 41.0 62-63 34.1894044586266 35.0 33.0 39.0 25.0 41.0 64-65 33.9483106087424 35.0 33.0 39.0 25.0 41.0 66-67 33.704936346737824 35.0 33.0 39.0 25.0 40.5 68-69 33.46854067909827 35.0 32.0 38.0 25.0 40.0 70-71 33.23900229099538 35.0 32.0 37.0 25.0 40.0 72-73 33.143192446828095 35.0 32.0 37.0 25.0 39.5 74-75 33.098498179267864 35.0 32.5 36.5 25.0 39.0 76-77 32.92111447190915 35.0 32.0 36.0 25.0 39.0 78-79 32.701231794268 35.0 32.0 36.0 25.0 39.0 80-81 32.491020870264116 35.0 32.0 35.5 25.0 37.5 82-83 32.288667250403904 35.0 32.0 35.0 25.0 37.0 84-85 32.09505368330741 35.0 32.0 35.0 25.0 37.0 86-87 31.912087586613517 35.0 32.0 35.0 24.5 36.0 88-89 31.744952588863672 35.0 31.0 35.0 24.0 36.0 90-91 31.579028944356807 35.0 31.0 35.0 24.0 36.0 92-93 31.437167894112946 35.0 31.0 35.0 23.5 36.0 94-95 31.292455608832526 35.0 31.0 35.0 23.0 35.5 96-97 31.151323232889094 35.0 31.0 35.0 22.5 35.0 98-99 31.036165057275632 35.0 30.5 35.0 20.5 35.0 100 30.327640686121367 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3375260192060665 1101 2 0.3002017289438186 1101 3 0.276870770509845 1101 4 0.24910755370783733 1101 5 0.21996070151222114 1101 6 0.16249988334177345 1101 7 0.1245508475267485 1101 8 0.16299972529814255 1101 9 0.29209451624066674 1101 10-11 0.3197383818747568 1101 12-13 0.31877676065626304 1101 14-15 0.47217131688768177 1101 16-17 0.4867958170615694 1101 18-19 0.4903838385982695 1101 20-21 0.472985771324673 1101 22-23 0.4586198475006569 1101 24-25 0.5056777245508357 1101 26-27 0.5184667308019328 1101 28-29 0.5439504575005785 1101 30-31 0.5293462827924387 1101 32-33 0.5314813444294444 1101 34-35 0.5464208508473476 1101 36-37 0.5466519194618797 1101 38-39 0.53319755732538 1101 40-41 0.5702012736302251 1101 42-43 0.5520351769885323 1101 44-45 0.5208437557602821 1101 46-47 0.5175054578997162 1101 48-49 0.5275361889684831 1101 50-51 0.5215915291897062 1101 52-53 0.5243746868734576 1101 54-55 0.5518468908050593 1101 56-57 0.5326431197754715 1101 58-59 0.5351543454176806 1101 60-61 0.4896996443839967 1101 62-63 0.4236993509974525 1101 64-65 0.4248106861814591 1101 66-67 0.4905849498063404 1101 68-69 0.4776999094541594 1101 70-71 0.39836660162450954 1101 72-73 0.4226009450916379 1101 74-75 0.40653344228024224 1101 76-77 0.44296700121601873 1101 78-79 0.40688476217871994 1101 80-81 0.37579246605155703 1101 82-83 0.34400013704787114 1101 84-85 0.33579655495331195 1101 86-87 0.3116574451419716 1101 88-89 0.3426687239333859 1101 90-91 0.33746838895496367 1101 92-93 0.36608222988889594 1101 94-95 0.3694722576828582 1101 96-97 0.37636689765340137 1101 98-99 0.3702214622071729 1101 100 0.33183673021655835 1102 1 0.2545627134318664 1102 2 0.1643875107203776 1102 3 0.18382565368328585 1102 4 0.22466623143563424 1102 5 0.16511543174108567 1102 6 0.04935490134921139 1102 7 0.09007558225459888 1102 8 0.14962541335987822 1102 9 0.20909615739974186 1102 10-11 0.2565535437574198 1102 12-13 0.25560998612159835 1102 14-15 0.3604507804424131 1102 16-17 0.380240495390197 1102 18-19 0.3626671190472095 1102 20-21 0.326669205944917 1102 22-23 0.33744241869923997 1102 24-25 0.3240973094274082 1102 26-27 0.3342968229308383 1102 28-29 0.3515086247630137 1102 30-31 0.35036701120667857 1102 32-33 0.35344392482669207 1102 34-35 0.3376816863735286 1102 36-37 0.3697365601492919 1102 38-39 0.3744266984629121 1102 40-41 0.35250357912140373 1102 42-43 0.3722759349194362 1102 44-45 0.34051183540601926 1102 46-47 0.36123730002426413 1102 48-49 0.4000961734715034 1102 50-51 0.38086999115455455 1102 52-53 0.3636487000208035 1102 54-55 0.36767849161851984 1102 56-57 0.33796500313756184 1102 58-59 0.3385169792895013 1102 60-61 0.33444064101564663 1102 62-63 0.30706892785680395 1102 64-65 0.3039194039621833 1102 66-67 0.3214124935302962 1102 68-69 0.29772850817755625 1102 70-71 0.27418237863355444 1102 72-73 0.33229455888785253 1102 74-75 0.3191474241935097 1102 76-77 0.31154849621132996 1102 78-79 0.24792596079248597 1102 80-81 0.2497527500825214 1102 82-83 0.2769098057295025 1102 84-85 0.19148355799765682 1102 86-87 0.20011227119385566 1102 88-89 0.1957498683591652 1102 90-91 0.19173624098083764 1102 92-93 0.15109132570216133 1102 94-95 0.1567846254640557 1102 96-97 0.18485565476295207 1102 98-99 0.14873498202414126 1102 100 0.14369067182879647 1103 1 -0.07202145392605175 1103 2 -0.027941947976316328 1103 3 0.02687470220126542 1103 4 0.03513159188835857 1103 5 -0.004034004906216637 1103 6 -0.0843140735029877 1103 7 0.03674219465481343 1103 8 0.04198832560364707 1103 9 0.06477647814328691 1103 10-11 0.10183205398440975 1103 12-13 0.09612305951274891 1103 14-15 0.16117688306424327 1103 16-17 0.17924684638656885 1103 18-19 0.1408985108211951 1103 20-21 0.11708549753635111 1103 22-23 0.13437508653149166 1103 24-25 0.13981533104858102 1103 26-27 0.12408437311904663 1103 28-29 0.13511568147472985 1103 30-31 0.11981014760082331 1103 32-33 0.11322510828413357 1103 34-35 0.13693891500270894 1103 36-37 0.16434930584194518 1103 38-39 0.11780493493604638 1103 40-41 0.1275642932112575 1103 42-43 0.12016778335730294 1103 44-45 0.08953276213497219 1103 46-47 0.15367322268466665 1103 48-49 0.16158946452320322 1103 50-51 0.1319506685443983 1103 52-53 0.14467973475108664 1103 54-55 0.11434615606489729 1103 56-57 0.12940081573647433 1103 58-59 0.12200977889479958 1103 60-61 0.10979027435722344 1103 62-63 0.10895101496602422 1103 64-65 0.09551695626007728 1103 66-67 0.07439702897080025 1103 68-69 0.08533798061472453 1103 70-71 0.11097786738290694 1103 72-73 0.08562921542326762 1103 74-75 0.05256971670320354 1103 76-77 0.10548538490105841 1103 78-79 0.061561144265823486 1103 80-81 0.11064069106157604 1103 82-83 0.09051680514404836 1103 84-85 0.07075506006972176 1103 86-87 0.06434340955762607 1103 88-89 0.11887347964045958 1103 90-91 0.13829030725480607 1103 92-93 0.09681983826020968 1103 94-95 0.07655175984018214 1103 96-97 0.11976175159231062 1103 98-99 0.08949932781338532 1103 100 0.16414490244645918 1104 1 0.02378543418230805 1104 2 0.035929341653989866 1104 3 0.13857816170732207 1104 4 0.10620762053098787 1104 5 0.04753610137656494 1104 6 -0.0029502578147813097 1104 7 0.03602543389964552 1104 8 0.06320995312869826 1104 9 0.04385284449067939 1104 10-11 0.09684814237903083 1104 12-13 0.11860425908504624 1104 14-15 0.12524459410524003 1104 16-17 0.20091283277316307 1104 18-19 0.22514857007862332 1104 20-21 0.22805046882233881 1104 22-23 0.17179761206330824 1104 24-25 0.20491436531860074 1104 26-27 0.1972451124008643 1104 28-29 0.18033259175971494 1104 30-31 0.17287924662323206 1104 32-33 0.18790380762405334 1104 34-35 0.19812472889420718 1104 36-37 0.22001930304411133 1104 38-39 0.14111325478349102 1104 40-41 0.19648795596356194 1104 42-43 0.23200380094204576 1104 44-45 0.19954992663144822 1104 46-47 0.22176440859339408 1104 48-49 0.19558774189527384 1104 50-51 0.19759315510301434 1104 52-53 0.18062249280492892 1104 54-55 0.1899222566999299 1104 56-57 0.21016198967262767 1104 58-59 0.22650059582430515 1104 60-61 0.18087304659206893 1104 62-63 0.1929998121306582 1104 64-65 0.16512341930896923 1104 66-67 0.16325570487094154 1104 68-69 0.12077406413406777 1104 70-71 0.11112937733436468 1104 72-73 0.135803138628674 1104 74-75 0.1679302157673277 1104 76-77 0.1717085478830569 1104 78-79 0.19276838690240083 1104 80-81 0.16098303416980286 1104 82-83 0.14970098154431355 1104 84-85 0.1667127260034036 1104 86-87 0.15252892173283428 1104 88-89 0.1534385740101314 1104 90-91 0.18530121540908695 1104 92-93 0.1454137792028405 1104 94-95 0.12696372289246227 1104 96-97 0.2283897916958928 1104 98-99 0.20247099969076388 1104 100 0.19823587100331252 1105 1 0.03194844907584127 1105 2 0.031096545316508184 1105 3 0.02647766778513372 1105 4 0.07823891451537435 1105 5 0.07572706026643772 1105 6 0.0949259733687029 1105 7 0.012221936884920126 1105 8 0.0070284994695484215 1105 9 0.02902678670534442 1105 10-11 0.015249278849140069 1105 12-13 0.0384998043777216 1105 14-15 0.1069728741362681 1105 16-17 0.09262170426833904 1105 18-19 0.12226847589237622 1105 20-21 0.12647002549955033 1105 22-23 0.05647741324085587 1105 24-25 0.111340582812268 1105 26-27 0.09705135823595867 1105 28-29 0.12269826855788324 1105 30-31 0.10572845254468888 1105 32-33 0.11483399370852965 1105 34-35 0.11336646544300066 1105 36-37 0.0979605812587181 1105 38-39 0.06209672623238305 1105 40-41 0.05458289668616345 1105 42-43 0.08277138981642196 1105 44-45 0.11494057050501993 1105 46-47 0.12139843952770235 1105 48-49 0.10946605892809202 1105 50-51 0.09329523079475166 1105 52-53 0.07311254962522185 1105 54-55 0.0688211939707628 1105 56-57 0.03608634916949427 1105 58-59 0.041185528607890376 1105 60-61 0.07074742145661617 1105 62-63 0.06539712624584837 1105 64-65 0.044602160071335106 1105 66-67 0.05999852839169506 1105 68-69 0.012241502699026796 1105 70-71 0.06546172244593151 1105 72-73 0.08735525686348922 1105 74-75 0.040659451649716516 1105 76-77 0.029888386542396006 1105 78-79 0.02701808561094765 1105 80-81 0.023424475252937782 1105 82-83 0.0158081112464572 1105 84-85 0.03196206833583659 1105 86-87 0.026575065568433587 1105 88-89 0.046489938538485376 1105 90-91 0.036648129221944714 1105 92-93 0.006670832133696791 1105 94-95 0.043404999341674966 1105 96-97 0.026325645044831703 1105 98-99 0.06110458834284671 1105 100 0.05904251943574579 1106 1 0.1257164209668602 1106 2 0.07594091822229743 1106 3 0.05087320390477501 1106 4 0.0414356382295793 1106 5 -0.004256248860649237 1106 6 -8.417511796281474E-4 1106 7 -0.004186680754635574 1106 8 -0.007642663621254542 1106 9 0.03834588046839116 1106 10-11 0.06194092326866496 1106 12-13 0.03691364139940845 1106 14-15 0.058712056885809716 1106 16-17 0.05389132781247952 1106 18-19 0.09751929074720778 1106 20-21 0.10032062287163512 1106 22-23 0.09369206327990298 1106 24-25 0.11869473553073107 1106 26-27 0.09176050627796428 1106 28-29 0.0908448600087084 1106 30-31 0.08668144193145366 1106 32-33 0.10817087923633295 1106 34-35 0.08583991198525354 1106 36-37 0.11415726814595217 1106 38-39 0.08338775174633639 1106 40-41 0.09732462715830792 1106 42-43 0.09675108724456294 1106 44-45 0.09217321419319546 1106 46-47 0.12267089685635568 1106 48-49 0.11048636133395462 1106 50-51 0.0876611634741451 1106 52-53 0.12744479735596315 1106 54-55 0.10701238672319846 1106 56-57 0.047444278600053735 1106 58-59 0.10930054961779945 1106 60-61 0.058771434091283936 1106 62-63 0.08634007436283042 1106 64-65 0.0571817880217651 1106 66-67 0.0491407937136259 1106 68-69 0.06933015874043491 1106 70-71 0.10759385063508375 1106 72-73 0.09293232426605869 1106 74-75 0.09729776296888559 1106 76-77 0.13095758206374342 1106 78-79 0.14937467429852802 1106 80-81 0.11307268013563032 1106 82-83 0.11787753996839712 1106 84-85 0.129442728394217 1106 86-87 0.0774770754391021 1106 88-89 0.05527378940492511 1106 90-91 0.07856103568207118 1106 92-93 0.02979667737381675 1106 94-95 0.06234529279619849 1106 96-97 0.11649034459832919 1106 98-99 0.12552258343717781 1106 100 0.08411860757618683 1107 1 0.024152243305373133 1107 2 0.06765235759451471 1107 3 0.09473309244198447 1107 4 0.1202650897005384 1107 5 0.11947487308889038 1107 6 0.05634213727964266 1107 7 0.07955222127550599 1107 8 0.07133332448952956 1107 9 0.0743228563693421 1107 10-11 0.07145257056855314 1107 12-13 0.0962385321923449 1107 14-15 0.17844455895463085 1107 16-17 0.15191739892210165 1107 18-19 0.12037492023821272 1107 20-21 0.11374927199845786 1107 22-23 0.12080373634739772 1107 24-25 0.08046545790762138 1107 26-27 0.09403391625419033 1107 28-29 0.12044765198843521 1107 30-31 0.10107721041426032 1107 32-33 0.11444373111518047 1107 34-35 0.10321101918682274 1107 36-37 0.09214445825333684 1107 38-39 0.06483185325699026 1107 40-41 0.09981915706500644 1107 42-43 0.10652663529715056 1107 44-45 0.08997033819068179 1107 46-47 0.09778893444571679 1107 48-49 0.06172511294201399 1107 50-51 0.04608444212826868 1107 52-53 0.07289735784744522 1107 54-55 0.07254682366351517 1107 56-57 0.06950608469924191 1107 58-59 0.07771223771005964 1107 60-61 0.09647474456014038 1107 62-63 0.1041584579615602 1107 64-65 0.10308383802600929 1107 66-67 0.11146715876453328 1107 68-69 0.08343483498998694 1107 70-71 0.14081344653165218 1107 72-73 0.10030800709006371 1107 74-75 0.06533289498025852 1107 76-77 0.10366842891410499 1107 78-79 0.14223531220255126 1107 80-81 0.13525177105297814 1107 82-83 0.14549864327659634 1107 84-85 0.08236411657239273 1107 86-87 0.05632485905603701 1107 88-89 0.07949176427427318 1107 90-91 0.057409435154653465 1107 92-93 0.05443858031046034 1107 94-95 0.10014840888409893 1107 96-97 0.0988166540372113 1107 98-99 0.1298893634096565 1107 100 0.09818952674248393 1108 1 0.047357503812222035 1108 2 0.018077251233385994 1108 3 0.048532750205627195 1108 4 0.06780612789185625 1108 5 0.060315739783071365 1108 6 0.08462352584003696 1108 7 0.023165462863957487 1108 8 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60-61 -0.3107991615073473 2214 62-63 -0.28777163824308616 2214 64-65 -0.28406340392415785 2214 66-67 -0.3334557543943859 2214 68-69 -0.34705773784634175 2214 70-71 -0.2950693419880963 2214 72-73 -0.3223725002477238 2214 74-75 -0.33806795544957424 2214 76-77 -0.33913140995117885 2214 78-79 -0.3106453709495298 2214 80-81 -0.25027831444479887 2214 82-83 -0.3024166104085495 2214 84-85 -0.2786732140178323 2214 86-87 -0.24161242366674784 2214 88-89 -0.250054185101078 2214 90-91 -0.261431694207932 2214 92-93 -0.27738977316067803 2214 94-95 -0.24006825557857425 2214 96-97 -0.2305859287557972 2214 98-99 -0.2901601461051939 2214 100 -0.29777772946810543 2215 1 -0.08204689635347151 2215 2 -0.14111921901831437 2215 3 -0.21020939019668816 2215 4 -0.14230182504029187 2215 5 -0.057713910562775084 2215 6 -0.02079448614466628 2215 7 -0.08446949942958071 2215 8 -0.09671844937464158 2215 9 -0.1864036082679732 2215 10-11 -0.23300143049239352 2215 12-13 -0.2665574531299342 2215 14-15 -0.3589375674701287 2215 16-17 -0.3693279799843481 2215 18-19 -0.3499492629149543 2215 20-21 -0.33633125428475097 2215 22-23 -0.3395871651593012 2215 24-25 -0.3532183496104153 2215 26-27 -0.33668429921770837 2215 28-29 -0.347209058336432 2215 30-31 -0.33337952718611774 2215 32-33 -0.32398547367473896 2215 34-35 -0.35680370252054416 2215 36-37 -0.3683943643851677 2215 38-39 -0.3573965227335876 2215 40-41 -0.4149087070602704 2215 42-43 -0.38475579984066854 2215 44-45 -0.3315529879169219 2215 46-47 -0.3969243433910563 2215 48-49 -0.3649973564795346 2215 50-51 -0.35474047740985526 2215 52-53 -0.34577991930599694 2215 54-55 -0.3035020897469707 2215 56-57 -0.2947052673435806 2215 58-59 -0.313830165678624 2215 60-61 -0.28120613982804343 2215 62-63 -0.34410376159782174 2215 64-65 -0.3958771038804372 2215 66-67 -0.30251620724608586 2215 68-69 -0.33531822279474 2215 70-71 -0.33240749873870357 2215 72-73 -0.3265590369398126 2215 74-75 -0.2966045557693633 2215 76-77 -0.2967454434653689 2215 78-79 -0.2771866847689779 2215 80-81 -0.26981149202254073 2215 82-83 -0.29525035841381 2215 84-85 -0.27996675591271725 2215 86-87 -0.2591251920286304 2215 88-89 -0.2834524687429898 2215 90-91 -0.23415663357784666 2215 92-93 -0.2158471822364092 2215 94-95 -0.2584664263953407 2215 96-97 -0.2115693939618204 2215 98-99 -0.22655345229349066 2215 100 -0.21940154438530968 2216 1 0.5861921375530663 2216 2 0.4204411475715695 2216 3 0.222931665337466 2216 4 0.16369678095668405 2216 5 0.22683219658681253 2216 6 0.2867314846547302 2216 7 0.2351105865437333 2216 8 0.1470910929051712 2216 9 0.12662586825509692 2216 10-11 0.011127771608371972 2216 12-13 -0.0638575639978427 2216 14-15 -0.06863851650292219 2216 16-17 -0.08699516117447104 2216 18-19 0.028256444504854983 2216 20-21 0.030366831811384998 2216 22-23 0.05737557435048757 2216 24-25 0.050711407799568065 2216 26-27 0.03709914370530498 2216 28-29 0.05574805579521325 2216 30-31 0.08429195149435742 2216 32-33 0.06209989901978474 2216 34-35 0.06468575474389127 2216 36-37 0.07378740608682222 2216 38-39 0.057886939730146025 2216 40-41 0.0255510480077632 2216 42-43 -0.025119166240429536 2216 44-45 -0.004930218828647526 2216 46-47 0.011868608372147094 2216 48-49 0.016714103994274865 2216 50-51 0.02779996528013129 2216 52-53 -0.003986583192883586 2216 54-55 -0.02903269390212415 2216 56-57 -0.023608211352204478 2216 58-59 -0.0491014614696752 2216 60-61 -0.07270305929881005 2216 62-63 -0.10582754301320563 2216 64-65 -0.07625737080380901 2216 66-67 -0.04063668771872386 2216 68-69 -0.1031105087040558 2216 70-71 -0.10001917251496195 2216 72-73 -0.11432464755516492 2216 74-75 -0.10658184445111374 2216 76-77 -0.07416224184827058 2216 78-79 -0.08357877192119645 2216 80-81 -0.13241817630230912 2216 82-83 -0.12718656661391492 2216 84-85 -0.11899302246760612 2216 86-87 -0.12294630300555198 2216 88-89 -0.12556157857039096 2216 90-91 -0.09806146848222497 2216 92-93 -0.1670383804472415 2216 94-95 -0.15492051935454043 2216 96-97 -0.10652031294891273 2216 98-99 -0.11192719731611689 2216 100 -0.05748208390914655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15729.0 1 747.0 2 455.0 3 366.0 4 318.0 5 623.0 6 3171.0 7 8076.0 8 11064.0 9 14632.0 10 21942.0 11 29104.0 12 34082.0 13 38375.0 14 43128.0 15 50277.0 16 58341.0 17 66131.0 18 73023.0 19 79502.0 20 85141.0 21 91451.0 22 98957.0 23 110113.0 24 150054.0 25 192681.0 26 125150.0 27 266127.0 28 158300.0 29 268831.0 30 220780.0 31 265233.0 32 345495.0 33 617494.0 34 747238.0 35 2325592.0 36 1580276.0 37 2316100.0 38 2159342.0 39 690540.0 40 6190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.224974332793082 31.136425184539817 29.858979252356683 23.779621230310415 2 12.130452339916395 33.99751956375363 32.75906922142961 21.112958874900364 3 10.974350400356919 36.17557007058812 31.771276924858565 21.078802604196394 4 9.506894686604916 36.20024878742907 32.58496610539062 21.707890420575396 5 9.522033604278231 35.3918659004767 33.27243452373016 21.813665971514908 6 9.410517387299024 33.25547996053885 34.48950597095952 22.844496681202607 7 11.877268181361345 39.07475200817695 29.145365664274486 19.902614146187222 8 13.255818621205535 34.42929985419932 25.645153752757167 26.66972777183797 9 9.846075198020095 33.66908919855737 28.229225573914334 28.255610029508198 10-11 10.914662570895652 51.088352639323716 26.625438597903656 11.371546191876966 12-13 10.175734915020465 51.063416275244236 27.74174073460496 11.019108075130339 14-15 10.284917345545093 50.36054427118736 27.699018767416682 11.65551961585087 16-17 10.199480549866543 50.104907476994 27.873307552683013 11.822304420456451 18-19 10.164855803695918 49.86833946008992 28.233107764728654 11.733696971485509 20-21 10.07805208200929 49.689906896418194 28.43265013730608 11.799390884266439 22-23 10.132832888821811 49.701338066134255 28.68390820847968 11.481920836564258 24-25 10.079967234721009 49.409484943056995 28.73425234507987 11.776295477142128 26-27 10.341860012476568 49.421148844299466 28.53895871770203 11.698032425521935 28-29 10.323964931890929 49.50568458513777 28.62899983517514 11.541350647796161 30-31 10.418203016131683 49.580820261652946 28.666003059822025 11.334973662393345 32-33 10.38520087640286 49.57069884563064 28.67266717173346 11.371433106233043 34-35 10.31806819760331 49.65905467862536 28.698790741543668 11.324086382227662 36-37 10.510993913312253 49.52951659110131 28.60602794777626 11.353461547810175 38-39 10.583784196771461 49.561901558840205 28.7504372557641 11.103876988624235 40-41 10.586334985484923 49.83920967869088 28.70944359212173 10.865011743702471 42-43 10.615788291447918 49.81431932402512 28.797465865666823 10.77242651886014 44-45 10.701461527670203 49.73954711224869 28.715029180595224 10.843962179485883 46-47 10.70169883483056 49.659201280796935 28.763516012302393 10.875583872070106 48-49 10.71749404074126 49.620359761748745 28.82135301585549 10.840793181654504 50-51 10.791859303607279 49.62541428341006 28.894000033629897 10.688726379352772 52-53 10.846041104480305 49.52919029884172 29.043723431123674 10.581045165554302 54-55 10.859936148225819 49.4552540418191 29.003170567498216 10.681639242456864 56-57 10.904582705831237 49.41986936471784 28.853864033518445 10.821683895932484 58-59 10.865433984677672 49.23511949010962 28.98230895495167 10.917137570261039 60-61 10.87549316907372 49.27937057117806 29.090758539238333 10.754377720509886 62-63 10.920815960725601 49.10680170617773 29.09434402180383 10.878038311292839 64-65 11.019065871418762 49.06014188031237 28.98843895895923 10.932353289309638 66-67 10.990876272583288 48.89120835120268 29.195070229743227 10.922845146470813 68-69 10.939919585532646 49.100196656984785 29.108214363639846 10.851669393842728 70-71 10.787529534229751 49.362128037204705 29.066841906216883 10.783500522348659 72-73 10.798693401804933 49.400463479660786 29.113513049187585 10.687330069346704 74-75 10.822081099216167 49.44509815074657 29.029455278269502 10.70336547176777 76-77 10.790803398425613 49.608069834175964 28.99062891932974 10.610497848068684 78-79 10.670889631649187 49.85842281117009 28.930883477454177 10.539804079726556 80-81 10.597356534846378 50.03099214865567 28.888909940567864 10.482741375930082 82-83 10.5380380940727 50.04395539053947 28.926863157759154 10.491143357628681 84-85 10.548065907537998 50.24387352641453 28.730041346191896 10.478019219855577 86-87 10.605341971830775 50.409680229439644 28.496508661369262 10.48846913736031 88-89 10.681469091363311 50.480004055855474 28.47072007086065 10.36780678192056 90-91 10.689791267481906 50.49675965261049 28.54003318421282 10.273415895694777 92-93 10.77501203737185 50.520640905286754 28.411638688553097 10.292708368788302 94-95 10.908345073883556 50.41610881500793 28.310220129465897 10.365325981642616 96-97 11.056668781757965 50.22885113096223 28.346583391109835 10.367896696169975 98-99 11.144939231836489 50.19442442035729 28.336500779847174 10.324135567959047 100 11.321917010530685 50.14968991385893 28.238386421144202 10.290006654466188 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1182954.0 1 1014270.0 2 506281.0 3 108302.0 4 50417.0 5 50581.0 6 46874.5 7 40978.0 8 38016.5 9 40909.0 10 50897.5 11 69853.5 12 98028.5 13 133827.0 14 175246.0 15 219173.5 16 261605.5 17 299885.0 18 334175.5 19 366685.0 20 396985.5 21 427248.5 22 460903.5 23 491993.5 24 512746.0 25 520681.0 26 517610.0 27 506868.5 28 489713.0 29 464737.5 30 431246.5 31 392426.5 32 351529.0 33 311687.5 34 274212.0 35 239908.0 36 210249.5 37 183846.5 38 160820.5 39 141028.5 40 124055.5 41 110583.0 42 100880.5 43 94826.0 44 92149.5 45 92038.5 46 92475.0 47 92423.0 48 90954.0 49 86722.5 50 79605.5 51 69815.5 52 58666.0 53 47535.0 54 37267.5 55 28326.0 56 20752.0 57 14874.0 58 10357.0 59 7056.5 60 4956.0 61 3567.5 62 2688.0 63 2146.0 64 1807.5 65 1561.5 66 1351.0 67 1212.5 68 1087.0 69 971.0 70 906.0 71 855.0 72 786.5 73 760.0 74 723.5 75 671.0 76 602.0 77 553.5 78 538.5 79 496.0 80 465.0 81 441.5 82 418.0 83 384.5 84 375.0 85 380.0 86 373.0 87 345.0 88 333.0 89 325.0 90 317.0 91 304.5 92 271.0 93 262.5 94 264.5 95 244.0 96 224.5 97 215.5 98 202.5 99 178.5 100 199.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1602522510744253 2 0.15033465166601087 3 0.15207733693159198 4 0.14822547894114443 5 0.14666229773725406 6 0.12933267644819202 7 0.13098560968292777 8 0.132346848817416 9 0.13172606393740213 10-11 0.13116137407666664 12-13 0.13474023630662615 14-15 0.14290019177765192 16-17 0.13588083503195283 18-19 0.14337886927549395 20-21 0.14466531505094438 22-23 0.1449009141319135 24-25 0.14549552133626414 26-27 0.14618735990736395 28-29 0.14686797947460806 30-31 0.14505798018589292 32-33 0.14555909569144626 34-35 0.1474663263469106 36-37 0.14813946657825094 38-39 0.14854709038500702 40-41 0.14508789752950804 42-43 0.14228688623354183 44-45 0.14213729951546616 46-47 0.14291889011741138 48-49 0.1444072779622639 50-51 0.14176707238822897 52-53 0.14227940689763804 54-55 0.1451477322167383 56-57 0.14176333272027708 58-59 0.14260101834150063 60-61 0.14414924087358344 62-63 0.13890996607298442 64-65 0.13042091982219225 66-67 0.13058546521207545 68-69 0.12883530061059054 70-71 0.1273057764182672 72-73 0.13075001060195865 74-75 0.1321935224313885 76-77 0.1292578830891542 78-79 0.12474036420327012 80-81 0.12557057048858986 82-83 0.12610160333775836 84-85 0.12131856802728999 86-87 0.12541724410256233 88-89 0.12444119076711883 90-91 0.13040970081833658 92-93 0.12689067327560732 94-95 0.12889513529782082 96-97 0.13248521653163597 98-99 0.1360939961052106 100 0.13639316954136188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3370171E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.47624612506338 #Duplication Level Percentage of deduplicated Percentage of total 1 85.54567261001172 59.43392205187268 2 11.87377171610036 16.4989017236121 3 1.853944478031121 3.8641530857367696 4 0.36443977062165933 1.0127962880588819 5 0.12007334249523481 0.41711225481289826 6 0.05822227276330344 0.2427038971478306 7 0.03904735075100505 0.1899004345915954 8 0.02597132335830676 0.14435120430682627 9 0.018909848072726245 0.11824067329994559 >10 0.08919455996187053 1.0722333797287065 >50 0.006119627670437128 0.2945923904359316 >100 0.003349265340429975 0.44318813570927557 >500 4.329312125307745E-4 0.19328651804081812 >1k 4.416106046310523E-4 0.6565569924082044 >5k 1.2925042252077908E-4 0.6435098827847485 >10k+ 2.800425821283547E-4 14.774551087452927 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 813290 6.082869097186565 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 192934 1.4430181932602058 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 154154 1.1529695469115542 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148510 1.1107561750706105 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87130 0.6516745372964938 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60472 0.452290400773483 No Hit ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54472 0.40741438535079316 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52978 0.3962402575105434 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45064 0.3370487931680156 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43058 0.32204524534502965 No Hit CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35924 0.2686876630074514 No Hit AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28590 0.2138342134891169 No Hit AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26939 0.20148582991197347 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24406 0.1825406720676946 No Hit CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23135 0.1730344361339881 No Hit TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22449 0.16790361170399393 No Hit GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21648 0.16191266364506482 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21262 0.15902563998620511 No Hit AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18869 0.14112758916845566 No Hit AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16489 0.12332676971745536 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16295 0.12187577855212173 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 15031 0.11242189796974175 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14701 0.10995371712149381 No Hit CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14219 0.10634867721587106 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.55412926281945E-4 0.0 0.0 2.2438007711344903E-5 0.0 2 0.0016529332347357411 0.0 0.0 3.7396679518908175E-5 7.479335903781634E-6 3 0.0026776022535538254 0.0 0.0 5.2355351326471444E-5 7.479335903781634E-6 4 0.004323056152385785 0.0 0.0 6.731402313403471E-5 7.479335903781634E-6 5 0.007681277973183739 0.0 0.0 9.723136674916125E-5 7.479335903781634E-6 6 0.014270572904415359 0.0 0.0 1.1219003855672451E-4 7.479335903781634E-6 7 0.02168259478506296 0.0 0.0 1.1966937446050615E-4 7.479335903781634E-6 8 0.040567917942111585 0.0 0.0 1.271487103642878E-4 7.479335903781634E-6 9 0.1071489661575757 0.0 0.0 1.271487103642878E-4 7.479335903781634E-6 10-11 0.2523752314013037 0.0 0.0 1.271487103642878E-4 7.479335903781634E-6 12-13 0.41928035176214273 0.0 0.0 1.3462804626806943E-4 7.479335903781634E-6 14-15 0.5959160881338017 0.0 0.0 1.3836771421996024E-4 7.479335903781634E-6 16-17 0.7882472108995464 0.0 0.0 1.533263860275235E-4 7.479335903781634E-6 18-19 1.0053461545106641 0.0 0.0 1.9820240145021332E-4 7.479335903781634E-6 20-21 1.2958547800173985 0.0 0.0 2.3185941301723066E-4 7.479335903781634E-6 22-23 1.6201139087899474 0.0 0.0 2.3185941301723066E-4 7.479335903781634E-6 24-25 1.9446385539870805 0.0 0.0 2.3185941301723066E-4 7.479335903781634E-6 26-27 2.234575010297176 0.0 0.0 2.3185941301723066E-4 7.479335903781634E-6 28-29 2.501822901143149 0.0 0.0 2.393387489210123E-4 7.479335903781634E-6 30-31 2.7780534744095644 0.0 0.0 2.617767566323572E-4 7.479335903781634E-6 32-33 3.0869911835832164 0.0 0.0 2.729957604880297E-4 7.479335903781634E-6 34-35 3.378531957444673 0.0 0.0 3.0291310410315625E-4 7.479335903781634E-6 36-37 3.6098304202691196 0.0 0.0 3.253511118145011E-4 7.479335903781634E-6 38-39 3.7889418168249307 0.0 0.0 3.8144613109286336E-4 7.479335903781634E-6 40-41 3.948161919544634 0.0 0.0 4.412808183231164E-4 7.479335903781634E-6 42-43 4.088227443014753 0.0 0.0 4.8241716579391545E-4 7.479335903781634E-6 44-45 4.213184707959233 0.0 0.0 5.235535132647144E-4 7.479335903781634E-6 46-47 4.328437534568556 0.0 0.0 5.497311889279501E-4 7.479335903781634E-6 48-49 4.442239370012546 0.0 0.0 6.170452120619848E-4 7.479335903781634E-6 50-51 4.560397918620488 0.0 0.0 6.95578239051692E-4 7.479335903781634E-6 52-53 4.68603206346426 0.0 0.0 8.339459532716522E-4 7.479335903781634E-6 54-55 4.81049569223909 0.0 0.0 9.910120072510666E-4 7.479335903781634E-6 56-57 4.938470869220746 0.0 0.0 0.0010545863624332103 7.479335903781634E-6 58-59 5.076490794321179 0.0 0.0 0.0010770243701445554 7.479335903781634E-6 60-61 5.223841190961582 0.0 0.0 0.0011592970650861532 7.479335903781634E-6 62-63 5.366401072955611 0.0 0.0 0.0011929540766531708 7.479335903781634E-6 64-65 5.506627402147661 0.0 0.0 0.0012640077677390962 7.479335903781634E-6 66-67 5.646053442398006 0.0 0.0 0.001413594485814729 7.479335903781634E-6 68-69 5.781881922078632 0.0 0.0 0.0014509911653336372 7.479335903781634E-6 70-71 5.9166034600455 0.0 0.0 0.001492127512804436 7.479335903781634E-6 72-73 6.055427413755591 0.0 0.0 0.0015557018679865798 7.479335903781634E-6 74-75 6.197317895186232 0.0 0.0 0.001637974562928178 7.479335903781634E-6 76-77 6.344410254737953 0.0 0.0 0.0017539042694367933 7.479335903781634E-6 78-79 6.495649906048322 0.0 0.0 0.001899751319560535 7.479335903781634E-6 80-81 6.651683811673014 0.0 0.0 0.0021353504005296566 7.479335903781634E-6 82-83 6.8102943485165595 0.0 0.0 0.002255019774990163 7.479335903781634E-6 84-85 6.974720069025295 0.0 0.0 0.0022587594429420536 7.479335903781634E-6 86-87 7.1444598576936675 0.0 0.0 0.002269978446797726 7.479335903781634E-6 88 7.276414041376135 0.0 0.0 0.0022811974506533987 7.479335903781634E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGCC 115 1.8189894E-12 44.96269 3 CCATGGG 85 7.928975E-9 44.24252 2 GTAAGCC 85 7.930794E-9 44.24136 3 GTCCGCG 45 0.0010129117 41.78977 1 GTCCCGT 180 0.0 41.78977 1 GCCGTAC 130 5.456968E-12 39.77573 2 CCCGACG 2025 0.0 38.52791 8 CCGTTTG 575 0.0 38.428417 1 GAGTCCC 150 0.0 37.61079 1 GTCACGC 75 7.4411873E-6 37.606144 2 AGTGGCC 100 3.3274773E-8 37.60516 3 CTGCCAA 1030 0.0 37.420925 5 CATTGCC 340 0.0 37.328648 3 TTGCGCC 140 1.2732926E-11 36.93364 3 ATTGCCG 115 2.7775968E-9 36.786278 4 CCGTTGT 680 0.0 36.642868 1 GCCATGG 90 6.182927E-7 36.566048 1 CGAGCCT 65 1.4134646E-4 36.15745 4 TGGCGCC 105 5.1129973E-8 35.814434 3 CCTGCTG 925 0.0 35.567116 8 >>END_MODULE