FastQCFastQC Report
Thu 26 May 2016
SRR1248447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11085152
Sequences flagged as poor quality0
Sequence length100
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4683064.224624073715904No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1148371.0359533184569774No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA927270.8364973254313518No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890100.8029659854912229No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA526640.4750859528132767No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA361590.32619309144340103No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329210.2969828469650213No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA321080.28964871207900444No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA266560.24046580506970044No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255810.2307681482400963No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210820.1901823267736879No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186080.16786418445141754No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171390.15461222363031196No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147090.13269100865734634No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137070.12365189038454322No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135040.12182061193206913No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133090.12006150208855955No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126890.1144684348938111No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120950.10910991567819729No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116370.10497826281497989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTC2200.038.4638861
ACGTGCC1650.037.035113
TCCTGGC1700.035.946822
CCCGACG16100.035.905018
TTGCGCC1451.8189894E-1135.65983
GGATCCC801.157624E-535.258561
CCTGCTG8200.034.3884968
CCTGTTT8600.033.891951
CGCATGC1256.2682375E-933.8453142
TTGCCGC2100.033.5795821
AAGGTCC702.1842017E-433.57672
GCTGCCA1703.6379788E-1233.1792834
GGGACCT1001.4099478E-632.907991
CCGTTTG5600.032.740091
GGCTCCT1309.1822585E-932.5463641
GGTCCCT1457.4396667E-1032.4216651
GAGCCCC1457.4396667E-1032.4216651
CTGCTGA11400.032.157139
CTTGCCA7450.031.5462954
GGACTCC1201.6509694E-731.3409421
Produced by FastQC (version 0.11.5)