##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248447_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11085152
Sequences flagged as poor quality	0
Sequence length	100
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.51789276322057	33.0	31.0	34.0	25.0	34.0
2	30.843844811510028	33.0	31.0	34.0	26.0	34.0
3	31.21979301682106	33.0	31.0	34.0	27.0	34.0
4	34.53770963176689	37.0	35.0	37.0	31.0	37.0
5	34.36417976045795	37.0	35.0	37.0	30.0	37.0
6	34.22535198434807	37.0	35.0	37.0	30.0	37.0
7	34.398328412637014	37.0	35.0	37.0	30.0	37.0
8	34.44539452413463	37.0	35.0	37.0	31.0	37.0
9	35.88541032184313	39.0	37.0	39.0	30.0	39.0
10-11	36.38775589184523	39.0	37.0	39.0	31.0	39.0
12-13	36.47940131989169	39.0	37.0	39.0	32.0	39.0
14-15	37.75760201574142	40.0	38.0	41.0	32.0	41.0
16-17	37.670914751552345	40.0	38.0	41.0	32.0	41.0
18-19	37.38785931848296	40.0	37.0	41.0	31.5	41.0
20-21	37.166808898966835	40.0	36.0	41.0	31.0	41.0
22-23	36.95352296477306	40.0	35.0	41.0	30.0	41.0
24-25	36.84231691184749	40.0	35.0	41.0	30.0	41.0
26-27	36.744341484898	39.5	35.0	41.0	30.0	41.0
28-29	36.64802724401073	39.0	35.0	41.0	29.5	41.0
30-31	36.55976895941526	39.0	35.0	41.0	28.5	41.0
32-33	36.48355593139363	39.0	35.0	41.0	28.0	41.0
34-35	36.4087257892359	39.0	35.0	41.0	27.5	41.0
36-37	36.30038956615119	39.0	35.0	41.0	27.0	41.0
38-39	36.195737009289545	39.0	35.0	41.0	27.0	41.0
40-41	36.1074696134072	39.0	35.0	41.0	26.0	41.0
42-43	36.02480096799755	39.0	35.0	41.0	26.0	41.0
44-45	35.89586069726423	39.0	35.0	41.0	25.5	41.0
46-47	35.77830479906816	39.0	35.0	41.0	25.0	41.0
48-49	35.649719778312466	38.0	35.0	40.5	25.5	41.0
50-51	35.48120156584231	38.0	34.5	40.0	26.0	41.0
52-53	35.275488870157126	38.0	34.0	40.0	25.0	41.0
54-55	35.08265574527079	37.5	34.0	40.0	25.5	41.0
56-57	34.86296304281619	37.0	33.5	40.0	26.0	41.0
58-59	34.92896466372315	37.0	34.0	40.0	26.0	41.0
60-61	34.745106878101446	36.5	33.5	40.0	26.0	41.0
62-63	34.49192428755149	36.0	33.0	39.0	26.0	41.0
64-65	34.227216009306865	35.5	33.0	39.0	26.0	41.0
66-67	33.95562595803828	35.0	33.0	39.0	25.5	41.0
68-69	33.67661900351028	35.0	33.0	38.5	25.0	40.0
70-71	33.404128152685686	35.0	32.0	37.5	25.0	40.0
72-73	33.2778049863457	35.0	33.0	37.0	25.0	39.5
74-75	33.21333600116624	35.0	33.0	37.0	25.0	39.0
76-77	33.00930650296902	35.0	33.0	36.0	25.0	39.0
78-79	32.76790697141546	35.0	32.5	36.0	25.0	39.0
80-81	32.540356099763	35.0	32.0	35.5	25.0	37.0
82-83	32.32816153535829	35.0	32.0	35.0	25.0	37.0
84-85	32.133141836936474	35.0	32.0	35.0	25.0	36.5
86-87	31.947681051193527	35.0	32.0	35.0	24.5	36.0
88-89	31.786650692746477	35.0	32.0	35.0	24.0	36.0
90-91	31.62415431921908	35.0	32.0	35.0	24.0	36.0
92-93	31.4839017994521	35.0	31.0	35.0	23.5	36.0
94-95	31.345418538239258	35.0	31.0	35.0	23.0	35.0
96-97	31.209692298310387	35.0	31.0	35.0	22.0	35.0
98-99	31.089747889789876	35.0	31.0	35.0	20.5	35.0
100	30.353963662383702	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30302739819219937
1101	2	0.31245367964091386
1101	3	0.30016573805516344
1101	4	0.21515515798720486
1101	5	0.10057861266471946
1101	6	0.17697960080457875
1101	7	0.1758038731652931
1101	8	0.23910402254904994
1101	9	0.2990042762882297
1101	10-11	0.326877328747436
1101	12-13	0.33996826449025264
1101	14-15	0.43171099293365955
1101	16-17	0.4404451384958108
1101	18-19	0.4583016924339134
1101	20-21	0.4615432198787346
1101	22-23	0.4863317036290269
1101	24-25	0.5105586510729623
1101	26-27	0.5236155582700377
1101	28-29	0.5225170949291851
1101	30-31	0.5487452164279745
1101	32-33	0.5227128134157795
1101	34-35	0.5759175046861529
1101	36-37	0.5625618226219586
1101	38-39	0.5844820114932787
1101	40-41	0.5848516822704894
1101	42-43	0.566137414063185
1101	44-45	0.5285859277983604
1101	46-47	0.518802023427476
1101	48-49	0.5170692118272697
1101	50-51	0.49251045044137953
1101	52-53	0.5109951285225804
1101	54-55	0.5023008709578605
1101	56-57	0.4998229223368824
1101	58-59	0.46223663454453856
1101	60-61	0.4901608732561513
1101	62-63	0.4618224207900141
1101	64-65	0.45754849875900305
1101	66-67	0.3942745922468873
1101	68-69	0.38676798638194043
1101	70-71	0.3800144365845526
1101	72-73	0.36494201752093147
1101	74-75	0.34322722112030846
1101	76-77	0.34830279008737364
1101	78-79	0.3400627669711298
1101	80-81	0.3153363948922916
1101	82-83	0.2922088880410598
1101	84-85	0.24620986324548966
1101	86-87	0.24751356466030927
1101	88-89	0.2489896368497675
1101	90-91	0.25562210017135456
1101	92-93	0.2646222134481242
1101	94-95	0.2898084025645886
1101	96-97	0.28215608668124403
1101	98-99	0.3007220441268963
1101	100	0.3158030814263526
1102	1	0.1721591545878418
1102	2	0.1612739340812972
1102	3	0.2125348899979116
1102	4	0.14376594821345634
1102	5	0.044052754742821776
1102	6	-0.0415182549578077
1102	7	0.0811564924841548
1102	8	0.1314351390066406
1102	9	0.19475331429649145
1102	10-11	0.266875369583083
1102	12-13	0.26255104869085244
1102	14-15	0.3738519747465787
1102	16-17	0.38017780958575287
1102	18-19	0.4030820717161703
1102	20-21	0.34365037322857006
1102	22-23	0.34673992874749615
1102	24-25	0.2780696159550118
1102	26-27	0.33127532996057596
1102	28-29	0.3516301968521134
1102	30-31	0.3828942353252245
1102	32-33	0.38380619412652095
1102	34-35	0.41219477637376656
1102	36-37	0.4025258239409695
1102	38-39	0.4067187893337234
1102	40-41	0.3516882555030705
1102	42-43	0.35608953596112514
1102	44-45	0.32254243975822305
1102	46-47	0.3024832402089217
1102	48-49	0.32108192149797077
1102	50-51	0.32732465512338393
1102	52-53	0.32042879851380235
1102	54-55	0.318475103614702
1102	56-57	0.4018704408028242
1102	58-59	0.3567827042355418
1102	60-61	0.3153275799522177
1102	62-63	0.36234383346761234
1102	64-65	0.3316635117307172
1102	66-67	0.3460956702081148
1102	68-69	0.35361932861022183
1102	70-71	0.31004095729172576
1102	72-73	0.28738586605068406
1102	74-75	0.3285382102830212
1102	76-77	0.3221982693296468
1102	78-79	0.3084938788939269
1102	80-81	0.34276985325954
1102	82-83	0.3115653171830033
1102	84-85	0.22334780860190762
1102	86-87	0.21329435144072306
1102	88-89	0.23615289828134323
1102	90-91	0.2614463676821437
1102	92-93	0.17859662682723965
1102	94-95	0.18264226592439314
1102	96-97	0.18397023938153723
1102	98-99	0.19375386790862237
1102	100	0.14978704175723934
1103	1	0.06513085279985376
1103	2	0.11926639562849672
1103	3	0.16763313372397093
1103	4	0.10635959884555746
1103	5	0.037835767509349694
1103	6	0.03440616534389562
1103	7	0.09136159256499354
1103	8	0.12337719314200513
1103	9	0.07718953741113666
1103	10-11	0.17965819863817245
1103	12-13	0.18788991250646347
1103	14-15	0.2940075934887929
1103	16-17	0.3278392098168226
1103	18-19	0.28623967087013824
1103	20-21	0.32604710146465266
1103	22-23	0.34643410526744844
1103	24-25	0.312735821145786
1103	26-27	0.2928050437145089
1103	28-29	0.35121466647127875
1103	30-31	0.28936260535381564
1103	32-33	0.308872553876391
1103	34-35	0.3477593144725688
1103	36-37	0.3324757437843857
1103	38-39	0.3247845407781895
1103	40-41	0.3471858097752616
1103	42-43	0.31195095469887946
1103	44-45	0.28350834052521634
1103	46-47	0.3037935623397132
1103	48-49	0.3377834251594436
1103	50-51	0.32174879000084644
1103	52-53	0.29411132568332476
1103	54-55	0.2750599043087405
1103	56-57	0.2829597547484184
1103	58-59	0.2871930313071971
1103	60-61	0.23837983396586537
1103	62-63	0.26794472573102013
1103	64-65	0.2812277804846559
1103	66-67	0.2707016030155671
1103	68-69	0.22505535199566395
1103	70-71	0.22369198367926657
1103	72-73	0.26904764257601244
1103	74-75	0.2399447609818921
1103	76-77	0.26280249636839415
1103	78-79	0.21452039765830477
1103	80-81	0.22987197476315657
1103	82-83	0.19858744597100042
1103	84-85	0.18830270688972206
1103	86-87	0.17902567266356328
1103	88-89	0.1823063984893878
1103	90-91	0.22757196248026546
1103	92-93	0.24873927487666592
1103	94-95	0.19434322559794737
1103	96-97	0.13155136843432658
1103	98-99	0.17711819725899502
1103	100	0.16869588453659645
1104	1	0.031184666492197977
1104	2	0.057568904956049494
1104	3	0.15300195718147336
1104	4	0.12335557972042466
1104	5	0.02070257509122797
1104	6	-0.10801535324606704
1104	7	0.03190638689977021
1104	8	0.06454392743496129
1104	9	0.07511777396044295
1104	10-11	0.10763080453836693
1104	12-13	0.11998664698880646
1104	14-15	0.19211575231855704
1104	16-17	0.2478403117331851
1104	18-19	0.21104474932715078
1104	20-21	0.21595800119656872
1104	22-23	0.2452288039019379
1104	24-25	0.22408295406845724
1104	26-27	0.20419169640825885
1104	28-29	0.21743096691336916
1104	30-31	0.23080647161101808
1104	32-33	0.24151562078509414
1104	34-35	0.23481237117132991
1104	36-37	0.267960366025072
1104	38-39	0.2620993083513099
1104	40-41	0.2387016277272025
1104	42-43	0.29978013929799374
1104	44-45	0.2892095639393233
1104	46-47	0.3065794823738699
1104	48-49	0.32058795151045416
1104	50-51	0.28722061968131385
1104	52-53	0.2611526745207726
1104	54-55	0.3003439375980932
1104	56-57	0.30136291724836894
1104	58-59	0.2865027380998839
1104	60-61	0.24811065072120186
1104	62-63	0.2584184407214991
1104	64-65	0.25020133965728064
1104	66-67	0.25540735978245266
1104	68-69	0.21710440563890643
1104	70-71	0.23283434266410552
1104	72-73	0.23499608551654205
1104	74-75	0.247417624856773
1104	76-77	0.21400220206489706
1104	78-79	0.22411696427058558
1104	80-81	0.20981947303998538
1104	82-83	0.19542400480099786
1104	84-85	0.18411987830486254
1104	86-87	0.2120559139545506
1104	88-89	0.18150859956731225
1104	90-91	0.2233946664337907
1104	92-93	0.1679951714204897
1104	94-95	0.15315981334176598
1104	96-97	0.20260125941921459
1104	98-99	0.21147613297939571
1104	100	0.23059789547655996
1105	1	0.11521556599620908
1105	2	0.11706162807752207
1105	3	0.12123832747163377
1105	4	0.1267277601458403
1105	5	0.08855945605765214
1105	6	0.002025691004163832
1105	7	-0.03694491384982257
1105	8	0.0053959686179538835
1105	9	0.002491639112705002
1105	10-11	0.08802828601537982
1105	12-13	0.1020742657732967
1105	14-15	0.20392624360102474
1105	16-17	0.21374701342535474
1105	18-19	0.1865211076489217
1105	20-21	0.1701514257470933
1105	22-23	0.1739481495783224
1105	24-25	0.15371637545011652
1105	26-27	0.17422661324519595
1105	28-29	0.151020722335943
1105	30-31	0.16898555293083461
1105	32-33	0.16646112155758885
1105	34-35	0.17841089945672195
1105	36-37	0.16033032364497757
1105	38-39	0.12962861978996898
1105	40-41	0.17794258972808308
1105	42-43	0.1446589042949924
1105	44-45	0.20267107204682588
1105	46-47	0.24046696376358767
1105	48-49	0.19788191127097576
1105	50-51	0.23907809209988073
1105	52-53	0.2651109675964136
1105	54-55	0.23965494530447273
1105	56-57	0.21539068315700405
1105	58-59	0.21621600681187658
1105	60-61	0.23271809134577381
1105	62-63	0.20356565798687853
1105	64-65	0.1903086315371496
1105	66-67	0.20440053206262832
1105	68-69	0.24558437490678386
1105	70-71	0.23698367710625945
1105	72-73	0.20484193507463289
1105	74-75	0.22093729887684077
1105	76-77	0.21892353596152248
1105	78-79	0.20508978693252544
1105	80-81	0.18879416201042432
1105	82-83	0.18603657065607138
1105	84-85	0.12243145789454246
1105	86-87	0.1135121154219334
1105	88-89	0.16287573226346197
1105	90-91	0.16620636595252947
1105	92-93	0.12408156756088218
1105	94-95	0.1452531126818002
1105	96-97	0.1877855572764453
1105	98-99	0.2113758351330013
1105	100	0.2675297555183036
1106	1	-0.012026103407368538
1106	2	-0.026810366185163303
1106	3	0.018430088759885166
1106	4	0.012861176313954559
1106	5	-0.046936375342369274
1106	6	-0.05699891487435593
1106	7	-0.04154056968920372
1106	8	0.02252311520454242
1106	9	0.03550257351449915
1106	10-11	0.01801562510342336
1106	12-13	0.02003483570452147
1106	14-15	0.10850334045179721
1106	16-17	0.1263611398269049
1106	18-19	0.12212842708321858
1106	20-21	0.12595999093774424
1106	22-23	0.03385365112573879
1106	24-25	0.05257341076973887
1106	26-27	0.0873067990480223
1106	28-29	0.050122824081462625
1106	30-31	0.09038136036305389
1106	32-33	0.08919161570295842
1106	34-35	0.0710626818714104
1106	36-37	0.056062816894538514
1106	38-39	0.08882474697158216
1106	40-41	0.0886535440664673
1106	42-43	0.0784672168850804
1106	44-45	0.07290005266136035
1106	46-47	0.07680797483713064
1106	48-49	0.06772912207078008
1106	50-51	0.09870368492485682
1106	52-53	0.08472835496708342
1106	54-55	0.08657069497342462
1106	56-57	0.01405449286914262
1106	58-59	0.032743377439231836
1106	60-61	0.06961618091997934
1106	62-63	0.11823414698611856
1106	64-65	0.11860295767193918
1106	66-67	0.09097590573549041
1106	68-69	0.13053312048252508
1106	70-71	0.11598550017028941
1106	72-73	0.05499782732798053
1106	74-75	0.08481696380886916
1106	76-77	0.06455573671297543
1106	78-79	0.09552512952122072
1106	80-81	0.05963750732277617
1106	82-83	0.10438707440643213
1106	84-85	0.07259724935881451
1106	86-87	0.06642703072882483
1106	88-89	0.024013925329125385
1106	90-91	0.056435621274228964
1106	92-93	0.09587534277775944
1106	94-95	0.11238229518984255
1106	96-97	0.11686739896205367
1106	98-99	0.07878336183303603
1106	100	0.04311326239260893
1107	1	-0.1014107342144932
1107	2	-0.0460274066273918
1107	3	-0.0248874199758653
1107	4	-0.09256161253865969
1107	5	-0.0522166712122214
1107	6	-0.07076738883895928
1107	7	-0.07228933809156501
1107	8	-0.054000449840991394
1107	9	-0.10226737550308229
1107	10-11	-0.010585097386119457
1107	12-13	0.027771613931022898
1107	14-15	0.0022615749709018473
1107	16-17	0.006271866899133727
1107	18-19	0.0015741723429982812
1107	20-21	-0.02722426179754933
1107	22-23	-0.03677838401775091
1107	24-25	-0.05223125211620783
1107	26-27	-0.03313351500938211
1107	28-29	-0.0640136075791844
1107	30-31	0.019276108888000465
1107	32-33	-0.0023867088946261106
1107	34-35	-0.0068911833402509615
1107	36-37	0.04653708939267176
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2216	40-41	-0.11349675552914817
2216	42-43	-0.10854904431045043
2216	44-45	-0.13033909638184582
2216	46-47	-0.16359649529122322
2216	48-49	-0.11494502389883365
2216	50-51	-0.1076236282626084
2216	52-53	-0.17164239926441383
2216	54-55	-0.1576583195372976
2216	56-57	-0.18093303948705142
2216	58-59	-0.18079245404288713
2216	60-61	-0.1911136286195898
2216	62-63	-0.2165054698343596
2216	64-65	-0.24632196054724886
2216	66-67	-0.24070326707516188
2216	68-69	-0.30826242684254623
2216	70-71	-0.30116267549835385
2216	72-73	-0.30102161269964256
2216	74-75	-0.2613132540893517
2216	76-77	-0.25822123042198086
2216	78-79	-0.2647416511060072
2216	80-81	-0.3037092240755328
2216	82-83	-0.24587626687077346
2216	84-85	-0.25145202061348115
2216	86-87	-0.21700422141275055
2216	88-89	-0.17130374612824895
2216	90-91	-0.1901046236126227
2216	92-93	-0.2771752323508636
2216	94-95	-0.18797941406444352
2216	96-97	-0.15952916322794053
2216	98-99	-0.15593174320189007
2216	100	-0.1236509380804307
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12620.0
1	635.0
2	325.0
3	296.0
4	271.0
5	603.0
6	3025.0
7	8375.0
8	10709.0
9	14435.0
10	20441.0
11	26561.0
12	29967.0
13	32148.0
14	35932.0
15	40755.0
16	46315.0
17	50538.0
18	55129.0
19	58792.0
20	63004.0
21	67606.0
22	72727.0
23	81040.0
24	105072.0
25	131735.0
26	97740.0
27	182913.0
28	127867.0
29	199716.0
30	182101.0
31	223620.0
32	292033.0
33	485536.0
34	624795.0
35	1678100.0
36	1420060.0
37	2051077.0
38	1992451.0
39	553522.0
40	4565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.29594252151689	28.812200073852996	30.128471142714723	24.76338626191539
2	13.619618256253773	30.769683176324214	33.3674707263423	22.243227841079715
3	12.308909308194146	32.2188311174408	33.01298927040875	22.4592703039563
4	11.146454349464786	31.85123004105798	33.78260300812415	23.219712601353084
5	11.1892674325671	31.196330138974876	34.4297180762373	23.184684352220728
6	10.916863211807739	29.21314742475284	35.685380726725654	24.184608636713765
7	13.561083682839437	34.80901941171333	30.185621138425372	21.444275767021864
8	14.816421288659523	30.409813217407077	26.461848879038886	28.311916614894518
9	11.304977830383647	29.730987458413903	29.357543594946367	29.606491116256077
10-11	12.685129471908743	47.42730140028477	27.34952120027789	12.538047927528591
12-13	11.801926292445724	47.03510537773729	28.796109224197895	12.366859105619096
14-15	11.874091223130558	46.3643143739232	28.722597576980448	13.038996825965796
16-17	11.778180928681914	46.14393908462905	28.869212900652286	13.208667086036746
18-19	11.68590017725797	45.82023682214093	29.280221707064175	13.213641293536924
20-21	11.63779572371883	45.63113246877841	29.379692158705794	13.35137964879696
22-23	11.737068801781017	45.56041534708444	29.65301877428914	13.049497076845407
24-25	11.672012419672551	45.256499030898766	29.767722853760343	13.303765695668337
26-27	11.90669461077141	45.285814503366176	29.593268553708292	13.21422233215413
28-29	11.863114239979417	45.39173383612522	29.689898929399096	13.055252994496264
30-31	11.915710785144032	45.33703264564066	29.769924455435483	12.977332113779823
32-33	11.927725237560201	45.35206337197157	29.683291457995743	13.03691993247249
34-35	11.941262173527235	45.446359465759414	29.654562155030227	12.95781620568312
36-37	12.065626274504188	45.35432848216395	29.6094845764469	12.970560666884959
38-39	12.131689005737156	45.2848549937367	29.76006676516178	12.82338923536436
40-41	12.214672453034693	45.529313418877976	29.64386952152342	12.612144606563907
42-43	12.243569568695694	45.55212692766596	29.69763675506763	12.506666748570714
44-45	12.270260392031599	45.48891663574203	29.669273248849766	12.571549723376602
46-47	12.275264819903134	45.4690537604695	29.67449061681997	12.581190802807395
48-49	12.300692734224473	45.37390425410008	29.7715097687283	12.553893242947145
50-51	12.391955944570189	45.386640163718695	29.801913981017194	12.419489910693924
52-53	12.47689964471693	45.25680006934115	29.940779530593105	12.325520755348816
54-55	12.505021061469899	45.163892911785645	29.945256032466872	12.385829994277582
56-57	12.548893423267723	45.13426201780019	29.80980751935231	12.50703703957978
58-59	12.509807557995185	45.03108495089819	29.889060038582983	12.57004745252365
60-61	12.519739646695893	44.98674176878978	30.027533651010547	12.46598493350378
62-63	12.579929601654014	44.77840843985231	30.076275789921823	12.565386168571848
64-65	12.65983551659101	44.74686769130606	29.988279443855276	12.605017348247657
66-67	12.6266869482303	44.68326360473225	30.116166462916166	12.573882984121282
68-69	12.582170217295591	44.783239802474895	30.042944815476304	12.591645164753212
70-71	12.53822261223298	44.92076246832009	30.00665809239127	12.534356827055653
72-73	12.529120822146567	44.948431576365024	30.077545931232226	12.444901670256188
74-75	12.555309005109033	45.04421107359763	29.96903526715155	12.431444654141792
76-77	12.542913508919925	45.18425491463183	29.929809651600813	12.34302192484743
78-79	12.444349588811065	45.331850406203294	29.92405206887844	12.299747936107204
80-81	12.399096704180554	45.45103937501792	29.843418036697688	12.30644588410383
82-83	12.393946217279977	45.5233031327024	29.818595701822577	12.264154948195038
84-85	12.380049985043504	45.65268577485488	29.751532498948706	12.215731741152908
86-87	12.411784786196863	45.80096914520609	29.60904748681449	12.17819858178256
88-89	12.476305186533022	45.88536106594247	29.536891517754665	12.10144222976984
90-91	12.545993840676564	45.9201719490965	29.51408492145397	12.019749288772966
92-93	12.62759428042349	45.978284461237884	29.402261785397286	11.991859472941337
94-95	12.802086004464334	45.90416995858394	29.281266560259496	12.012477476692233
96-97	12.928787457127692	45.74446003177503	29.322731539829842	12.004020971267435
98-99	13.047427037090717	45.66730440839402	29.272715636637976	12.01255291787729
100	13.27911122472622	45.53929966221084	29.20930959399811	11.97227951906483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	695838.0
1	603146.5
2	305670.5
3	65235.0
4	29820.5
5	29583.0
6	27534.0
7	24710.0
8	23483.5
9	25568.5
10	31691.5
11	41929.0
12	57050.0
13	77317.0
14	101707.0
15	128145.0
16	154984.5
17	181681.5
18	208867.0
19	235195.0
20	261781.5
21	288778.0
22	315682.5
23	344387.5
24	369517.5
25	387750.0
26	400939.0
27	410994.0
28	418172.5
29	419886.0
30	413411.0
31	398255.0
32	377033.5
33	352916.0
34	327349.5
35	301313.0
36	275958.5
37	252072.0
38	229894.0
39	208507.0
40	187926.5
41	169403.5
42	152780.5
43	138346.5
44	126866.5
45	117461.5
46	109223.5
47	101537.5
48	93537.0
49	83949.0
50	72859.5
51	61572.5
52	50168.5
53	39360.0
54	30159.0
55	22544.0
56	16363.0
57	11564.0
58	8069.0
59	5627.5
60	4044.0
61	3013.0
62	2346.5
63	1913.0
64	1590.0
65	1395.0
66	1170.5
67	1034.5
68	988.5
69	876.0
70	803.0
71	725.5
72	663.5
73	626.5
74	581.0
75	565.0
76	521.0
77	493.5
78	480.5
79	433.5
80	403.5
81	393.5
82	372.5
83	342.5
84	321.5
85	315.0
86	309.0
87	289.0
88	275.0
89	261.0
90	255.0
91	257.5
92	227.0
93	216.5
94	224.5
95	209.0
96	195.5
97	194.0
98	189.0
99	186.0
100	195.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15533390971995692
2	0.14574450580379952
3	0.14726004659205394
4	0.1436877004483114
5	0.14200075921376631
6	0.12542904238029393
7	0.12677318272225768
8	0.12815340736870365
9	0.1275941006492288
10-11	0.12703930446781422
12-13	0.1303996553227236
14-15	0.13827505477597418
16-17	0.13152728983779383
18-19	0.1390147830178603
20-21	0.13970940587914357
22-23	0.13999356977694127
24-25	0.14088214577481661
26-27	0.14139183657562837
28-29	0.14218118073617755
30-31	0.14007024892396605
32-33	0.14063406618150118
34-35	0.1424833867862164
36-37	0.1434035365505137
38-39	0.14348923677365905
40-41	0.14047168681133104
42-43	0.1379142117311517
44-45	0.137372947163918
46-47	0.1381803334767083
48-49	0.13952447381867206
50-51	0.1370166146571558
52-53	0.1375398370721484
54-55	0.140101822690388
56-57	0.13672342968323756
58-59	0.13753081599602784
60-61	0.1391500991596687
62-63	0.13408476491797314
64-65	0.1263401710684707
66-67	0.12641685021549548
68-69	0.12494641480784387
70-71	0.12325045249717821
72-73	0.12661982442820813
74-75	0.12782864862836343
76-77	0.12539746861387197
78-79	0.12048549266622595
80-81	0.12155449018651256
82-83	0.1220461388350832
84-85	0.11763483261212837
86-87	0.1212116892939312
88-89	0.12063434042221524
90-91	0.12601090179007016
92-93	0.12298433075162163
94-95	0.12487875673693964
96-97	0.12805417553137746
98-99	0.13151375822361297
100	0.13204149117666586
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1085152E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.59720989155717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.67297852467827	71.26643124596794
2	8.09193318389602	12.720067417662639
3	0.8770018099731639	2.067896860012089
4	0.15646397212519744	0.4919052663036358
5	0.05970412526915796	0.23462888325859155
6	0.0335304181864662	0.15812383895720217
7	0.024265316065960545	0.13350302979448928
8	0.016840124500394467	0.10588694399659666
9	0.009468134883477383	0.06697520862464215
>10	0.05249538255415046	0.7181329596959805
>50	0.002499576597550114	0.13629206228098067
>100	0.001818798992462024	0.3083507532489386
>500	3.338037231421597E-4	0.1922953172906903
>1k	3.334144710429629E-4	0.6730277208079803
>5k	1.034733362646272E-4	0.6107578998450632
>10k+	2.2994074725472713E-4	10.11572459225253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	468306	4.224624073715904	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114837	1.0359533184569774	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92727	0.8364973254313518	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89010	0.8029659854912229	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52664	0.4750859528132767	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36159	0.32619309144340103	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32921	0.2969828469650213	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32108	0.28964871207900444	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26656	0.24046580506970044	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25581	0.2307681482400963	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21082	0.1901823267736879	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18608	0.16786418445141754	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17139	0.15461222363031196	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14709	0.13269100865734634	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13707	0.12365189038454322	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13504	0.12182061193206913	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13309	0.12006150208855955	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12689	0.1144684348938111	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12095	0.10910991567819729	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11637	0.10497826281497989	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.404964045599014E-4	0.0	0.0	1.804215224112398E-5	0.0
2	0.0014523932554104807	0.0	0.0	2.7063228361685974E-5	0.0
3	0.0025800277704807295	0.0	0.0	3.608430448224796E-5	0.0
4	0.0043842429945931275	0.0	0.0	9.021076120561992E-5	0.0
5	0.007911483757732866	0.0	0.0	9.021076120561992E-5	0.0
6	0.01409092090031783	0.0	0.0	9.021076120561992E-5	0.0
7	0.020775538305654265	0.0	0.0	9.021076120561992E-5	0.0
8	0.03736529729136777	0.0	0.0	9.021076120561992E-5	0.0
9	0.09915064764109685	0.0	0.0	9.021076120561992E-5	0.0
10-11	0.2288782327928386	0.0	0.0	9.472129926590091E-5	0.0
12-13	0.37748693026491653	0.0	0.0	1.082529134467439E-4	0.0
14-15	0.541530688979276	0.0	0.0	1.1727398956730589E-4	4.510538060280995E-6
16-17	0.7255967261432229	0.0	0.0	1.3982667986871088E-4	9.02107612056199E-6
18-19	0.9236589629082217	0.0	0.0	1.6237937017011584E-4	9.02107612056199E-6
20-21	1.1847559690656475	0.0	0.0	1.6237937017011584E-4	9.02107612056199E-6
22-23	1.4779274113697314	0.0	0.0	1.6688990823039685E-4	9.02107612056199E-6
24-25	1.7644097257304185	0.0	0.0	1.7140044629067783E-4	9.02107612056199E-6
26-27	2.0259938699983544	0.0	0.0	1.7591098435095883E-4	9.02107612056199E-6
28-29	2.277645809457552	0.0	0.0	1.8944259853180182E-4	9.02107612056199E-6
30-31	2.548219456079628	0.0	0.0	1.8944259853180182E-4	9.02107612056199E-6
32-33	2.851111107903617	0.0	0.0	1.984636746523638E-4	9.02107612056199E-6
34-35	3.1372506213717233	0.0	0.0	2.029742127126448E-4	9.02107612056199E-6
36-37	3.362155070133454	0.0	0.0	2.074847507729258E-4	9.02107612056199E-6
38-39	3.535427389719149	0.0	0.0	2.165058268934878E-4	9.02107612056199E-6
40-41	3.6893765642545993	0.0	0.0	2.3454797913461176E-4	9.02107612056199E-6
42-43	3.8271374176917012	0.0	0.0	2.525901313757357E-4	9.02107612056199E-6
44-45	3.9525890127622967	0.0	0.0	2.7965335973742175E-4	9.02107612056199E-6
46-47	4.07351653815843	0.0	0.0	3.0671658809910767E-4	9.02107612056199E-6
48-49	4.193126986440962	0.0	0.0	3.382903545210747E-4	9.02107612056199E-6
50-51	4.3201753119848965	0.0	0.0	4.420327299075376E-4	9.02107612056199E-6
52-53	4.454363819278257	0.0	0.0	5.277329530528765E-4	9.02107612056199E-6
54-55	4.589964124984483	0.0	0.0	5.999015620173724E-4	9.02107612056199E-6
56-57	4.7282842851410605	0.0	0.0	6.450069426201824E-4	9.02107612056199E-6
58-59	4.876455460421291	0.0	0.0	6.585385568010254E-4	9.02107612056199E-6
60-61	5.033255295010839	0.0	0.0	7.081544754641163E-4	9.02107612056199E-6
62-63	5.191340632947568	0.0	0.0	7.307071657655212E-4	9.02107612056199E-6
64-65	5.346264083704039	0.0	0.0	8.209179269711412E-4	9.02107612056199E-6
66-67	5.5022475109046765	0.0	0.0	9.201497642973231E-4	9.02107612056199E-6
68-69	5.657116835204425	0.0	0.0	9.517235307192902E-4	9.02107612056199E-6
70-71	5.81465639803586	0.0	0.0	0.001010360525502943	9.02107612056199E-6
72-73	5.976602756552188	0.0	0.0	0.001032913215804348	9.02107612056199E-6
74-75	6.144331624861797	0.0	0.0	0.001073508058346877	9.02107612056199E-6
76-77	6.316886768895907	0.0	0.0	0.0011637188195524968	9.02107612056199E-6
78-79	6.492933069388674	0.0	0.0	0.0013080560374814888	9.02107612056199E-6
80-81	6.67777942963705	0.0	0.0	0.0014298405651090758	9.02107612056199E-6
82-83	6.8689721169362405	0.0	0.0	0.0014704354076516045	9.02107612056199E-6
84-85	7.067562086654292	0.0	0.0	0.0014839670218324474	9.02107612056199E-6
86-87	7.271884950246961	0.0	0.0	0.0015110302501941337	9.02107612056199E-6
88	7.427963098746864	0.0	0.0	0.0015155407882544145	9.02107612056199E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCTTC	220	0.0	38.463886	1
ACGTGCC	165	0.0	37.03511	3
TCCTGGC	170	0.0	35.94682	2
CCCGACG	1610	0.0	35.90501	8
TTGCGCC	145	1.8189894E-11	35.6598	3
GGATCCC	80	1.157624E-5	35.25856	1
CCTGCTG	820	0.0	34.388496	8
CCTGTTT	860	0.0	33.89195	1
CGCATGC	125	6.2682375E-9	33.845314	2
TTGCCGC	210	0.0	33.579582	1
AAGGTCC	70	2.1842017E-4	33.5767	2
GCTGCCA	170	3.6379788E-12	33.179283	4
GGGACCT	100	1.4099478E-6	32.90799	1
CCGTTTG	560	0.0	32.74009	1
GGCTCCT	130	9.1822585E-9	32.546364	1
GGTCCCT	145	7.4396667E-10	32.421665	1
GAGCCCC	145	7.4396667E-10	32.421665	1
CTGCTGA	1140	0.0	32.15713	9
CTTGCCA	745	0.0	31.546295	4
GGACTCC	120	1.6509694E-7	31.340942	1
>>END_MODULE