##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11085152 Sequences flagged as poor quality 0 Sequence length 100 %GC 26 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82493979333797 34.0 31.0 34.0 31.0 34.0 2 33.02001966233751 34.0 33.0 34.0 31.0 34.0 3 33.09753930302444 34.0 33.0 34.0 31.0 34.0 4 36.42533525927294 37.0 37.0 37.0 35.0 37.0 5 36.330625416773714 37.0 37.0 37.0 35.0 37.0 6 36.24486330904619 37.0 37.0 37.0 35.0 37.0 7 36.283698861323685 37.0 37.0 37.0 35.0 37.0 8 36.05513365987223 37.0 36.0 37.0 35.0 37.0 9 37.854281835738476 39.0 38.0 39.0 35.0 39.0 10-11 38.04736299511274 39.0 39.0 39.0 36.0 39.0 12-13 38.11416108683038 39.0 39.0 39.0 37.0 39.0 14-15 39.67786206269432 41.0 40.0 41.0 37.0 41.0 16-17 39.69759413312511 41.0 40.0 41.0 37.0 41.0 18-19 39.625868278576604 41.0 39.5 41.0 37.0 41.0 20-21 39.407018279947806 41.0 39.0 41.0 36.0 41.0 22-23 39.105824800598135 41.0 38.5 41.0 35.0 41.0 24-25 38.855733777940074 41.0 38.0 41.0 35.0 41.0 26-27 38.736710691923754 41.0 38.0 41.0 35.0 41.0 28-29 38.65736888407123 40.0 38.0 41.0 35.0 41.0 30-31 38.591301770151645 40.0 37.5 41.0 35.0 41.0 32-33 38.53138491019338 40.0 37.0 41.0 35.0 41.0 34-35 38.472612328635634 40.0 37.0 41.0 35.0 41.0 36-37 38.40932036836301 40.0 37.0 41.0 35.0 41.0 38-39 38.32979976278178 40.0 37.0 41.0 35.0 41.0 40-41 38.249109890419184 40.0 36.5 41.0 35.0 41.0 42-43 38.14962866544365 40.0 36.0 41.0 34.5 41.0 44-45 38.06751680085217 40.0 36.0 41.0 34.0 41.0 46-47 37.990378796790516 40.0 35.5 41.0 34.0 41.0 48-49 37.922847201373514 40.0 35.0 41.0 34.0 41.0 50-51 37.76966093924558 40.0 35.0 41.0 34.0 41.0 52-53 37.551667672215956 39.5 35.0 41.0 33.0 41.0 54-55 37.40877093972189 39.0 35.0 41.0 33.0 41.0 56-57 37.228468630831586 39.0 35.0 41.0 33.0 41.0 58-59 37.01835518358251 39.0 35.0 41.0 33.0 41.0 60-61 36.74549943022883 38.0 35.0 40.5 33.0 41.0 62-63 36.44797585996115 37.0 35.0 40.0 32.0 41.0 64-65 36.17315409838314 37.0 35.0 40.0 32.0 41.0 66-67 35.8894099963627 36.0 35.0 39.5 32.0 41.0 68-69 35.61966218415408 36.0 35.0 39.0 31.5 41.0 70-71 35.41157744160837 35.0 35.0 39.0 32.0 40.5 72-73 35.183659186630905 35.0 35.0 38.0 32.0 40.0 74-75 34.906113646434434 35.0 35.0 37.0 31.0 39.5 76-77 34.64013222371692 35.0 35.0 37.0 31.0 39.0 78-79 34.386555457245876 35.0 34.5 36.0 31.0 39.0 80-81 34.16320466331901 35.0 34.0 36.0 31.0 38.0 82-83 33.96729476510561 35.0 34.0 36.0 31.0 37.0 84-85 33.786556693133306 35.0 34.0 35.0 31.0 37.0 86-87 33.63401268651977 35.0 34.0 35.0 30.5 36.0 88-89 33.497722448911844 35.0 34.0 35.0 30.0 36.0 90-91 33.386928388532695 35.0 34.0 35.0 30.0 36.0 92-93 33.26898435853654 35.0 34.0 35.0 30.0 36.0 94-95 33.15763329181233 35.0 34.0 35.0 29.5 36.0 96-97 33.07157317283516 35.0 34.0 35.0 29.0 35.5 98-99 32.97670541639844 35.0 34.0 35.0 29.0 35.0 100 32.39016145200355 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23107993304049756 1101 2 0.21359739898466046 1101 3 0.18813394139403528 1101 4 0.11312928334731254 1101 5 0.12907906804349523 1101 6 0.017115598210672545 1101 7 0.07510289360126166 1101 8 -0.0025491075724204393 1101 9 -0.02365562061113735 1101 10-11 0.06222858019374655 1101 12-13 0.1403379405129428 1101 14-15 0.2430000545403388 1101 16-17 0.23723624441458213 1101 18-19 0.25075337369879946 1101 20-21 0.2826203171887869 1101 22-23 0.2514547592441545 1101 24-25 0.2520855131484083 1101 26-27 0.24750562367323425 1101 28-29 0.24906599554849862 1101 30-31 0.29271710615091706 1101 32-33 0.2603268279987816 1101 34-35 0.27268611614552896 1101 36-37 0.2746766375219778 1101 38-39 0.25397330257050044 1101 40-41 0.24078645731609072 1101 42-43 0.28062403790105606 1101 44-45 0.31855067997584996 1101 46-47 0.27346644437457 1101 48-49 0.2681906005303105 1101 50-51 0.27840377568383445 1101 52-53 0.2504208760524165 1101 54-55 0.26976521695216604 1101 56-57 0.28494953779633647 1101 58-59 0.2745423626180141 1101 60-61 0.2982143264264465 1101 62-63 0.24720717001326875 1101 64-65 0.24331288926079253 1101 66-67 0.2471613044108807 1101 68-69 0.20680640273597817 1101 70-71 0.21739309199455903 1101 72-73 0.21752557467871725 1101 74-75 0.22466875875048942 1101 76-77 0.22439229788261628 1101 78-79 0.2318666678117438 1101 80-81 0.20252677982323775 1101 82-83 0.16604898761709563 1101 84-85 0.16897385214033278 1101 86-87 0.17499298524757023 1101 88-89 0.19600757170709215 1101 90-91 0.19181603600802788 1101 92-93 0.22149438607528538 1101 94-95 0.1993999339624679 1101 96-97 0.19785274923631846 1101 98-99 0.1779586917060456 1101 100 0.12930841464001475 1102 1 0.12472591422950075 1102 2 0.15045317397147784 1102 3 0.14791920675633463 1102 4 0.09363027421630932 1102 5 0.09885048362334459 1102 6 -0.045028683981328754 1102 7 -0.029973015276119952 1102 8 -0.07619646895452092 1102 9 -0.08422021761455767 1102 10-11 5.818038785321278E-5 1102 12-13 0.10680988280662973 1102 14-15 0.1338417698319816 1102 16-17 0.15871787145752592 1102 18-19 0.17623141555654342 1102 20-21 0.18029736590538903 1102 22-23 0.15444947562709643 1102 24-25 0.1409242345639754 1102 26-27 0.153904328691695 1102 28-29 0.12905354212090003 1102 30-31 0.1430790788501568 1102 32-33 0.1406170932970099 1102 34-35 0.16627651091846474 1102 36-37 0.1657793784263788 1102 38-39 0.15302970724691534 1102 40-41 0.11009314626781475 1102 42-43 0.1416810361682579 1102 44-45 0.16378025897798665 1102 46-47 0.17873090816389947 1102 48-49 0.1815170778975741 1102 50-51 0.18612720621937484 1102 52-53 0.166578420900791 1102 54-55 0.17880310817513845 1102 56-57 0.1447974859601473 1102 58-59 0.1770016309447584 1102 60-61 0.17973804291526818 1102 62-63 0.15636772159318468 1102 64-65 0.15078582309424604 1102 66-67 0.15572358833912148 1102 68-69 0.17799642463917564 1102 70-71 0.16447950605757455 1102 72-73 0.14369746303545128 1102 74-75 0.13281578846265774 1102 76-77 0.1250571250453305 1102 78-79 0.142626359429741 1102 80-81 0.15738851053446723 1102 82-83 0.12398702729903022 1102 84-85 0.09291855238950575 1102 86-87 0.11721116586807057 1102 88-89 0.10584894530958167 1102 90-91 0.13701699866155082 1102 92-93 0.16563557306859877 1102 94-95 0.1379771132227674 1102 96-97 0.15271465688184094 1102 98-99 0.17010172912570454 1102 100 0.15958294387781535 1103 1 0.1832950719747899 1103 2 0.16656409280096796 1103 3 0.15745669627239778 1103 4 0.09472253296628708 1103 5 0.12094079941849856 1103 6 0.04766487684040044 1103 7 0.07336878077609299 1103 8 -0.03561621188418229 1103 9 -0.11466697647536961 1103 10-11 -0.023419682876877346 1103 12-13 0.08822982805298096 1103 14-15 0.17152860750511678 1103 16-17 0.19174155840436669 1103 18-19 0.18032974787315936 1103 20-21 0.17423988849875371 1103 22-23 0.16361735892511575 1103 24-25 0.17914805916501564 1103 26-27 0.1924572838521783 1103 28-29 0.20318725767916845 1103 30-31 0.1870182340165769 1103 32-33 0.19183216763976674 1103 34-35 0.21321953166430063 1103 36-37 0.2238674512416594 1103 38-39 0.2042770610814273 1103 40-41 0.18740124759717958 1103 42-43 0.19437152897202736 1103 44-45 0.18236614419789277 1103 46-47 0.21490364324276356 1103 48-49 0.1910143307893648 1103 50-51 0.22502002630574935 1103 52-53 0.15115519891269003 1103 54-55 0.17704649662724137 1103 56-57 0.2092909888468597 1103 58-59 0.1911257136659117 1103 60-61 0.22652192347498357 1103 62-63 0.1737635263435351 1103 64-65 0.1619448648327193 1103 66-67 0.1524094315555189 1103 68-69 0.18097986633679142 1103 70-71 0.17772039881877078 1103 72-73 0.1600955434172704 1103 74-75 0.13793588658008815 1103 76-77 0.09179635793487506 1103 78-79 0.13231930997464758 1103 80-81 0.13162991721947037 1103 82-83 0.11236751918978882 1103 84-85 0.09939885195620235 1103 86-87 0.1191652247018169 1103 88-89 0.11832049252335253 1103 90-91 0.11039976445879773 1103 92-93 0.1251975488179511 1103 94-95 0.056712264293082626 1103 96-97 0.011781119391827133 1103 98-99 0.050393331295197186 1103 100 0.05100696626128354 1104 1 -0.20799510564609136 1104 2 -0.1892219953902199 1104 3 -0.1296483915394262 1104 4 -0.189572094215805 1104 5 -0.09469433770982505 1104 6 -0.030981832131438125 1104 7 0.011874341149344048 1104 8 -0.13993481722538093 1104 9 -0.020225191807043075 1104 10-11 0.05310627327948225 1104 12-13 0.08792612383488319 1104 14-15 0.1277070812247061 1104 16-17 0.16202070480944997 1104 18-19 0.17112024086021194 1104 20-21 0.15958045239297292 1104 22-23 0.15216475194596768 1104 24-25 0.17068778640628324 1104 26-27 0.17312142950256515 1104 28-29 0.1853564603795519 1104 30-31 0.1630023687166755 1104 32-33 0.14880079573668326 1104 34-35 0.15962482224022523 1104 36-37 0.17011226376953203 1104 38-39 0.14874331173641053 1104 40-41 0.16050714233317365 1104 42-43 0.18168585463077846 1104 44-45 0.15353379101532738 1104 46-47 0.17162345633020948 1104 48-49 0.1602332729611362 1104 50-51 0.15778671445596615 1104 52-53 0.11669993872556006 1104 54-55 0.1614375874039098 1104 56-57 0.17187750793325307 1104 58-59 0.18046168853763334 1104 60-61 0.15470173556381184 1104 62-63 0.09250513786177805 1104 64-65 0.09494998013506972 1104 66-67 0.09913077699539485 1104 68-69 0.1303417132863487 1104 70-71 0.13352738898588967 1104 72-73 0.132850064574356 1104 74-75 0.13315043600821497 1104 76-77 0.10450416242966298 1104 78-79 0.11095808744435942 1104 80-81 0.07088577932841389 1104 82-83 0.06029967557697802 1104 84-85 0.07842878677670484 1104 86-87 0.058318711894045805 1104 88-89 0.03777158895882593 1104 90-91 0.02729069597412348 1104 92-93 0.06426608514590271 1104 94-95 0.032929075519305684 1104 96-97 0.048618665355228075 1104 98-99 0.0914198237749062 1104 100 0.1148287221076103 1105 1 0.09571858334443561 1105 2 0.08320443834040958 1105 3 0.0787421949955629 1105 4 0.039917670354668644 1105 5 0.05819386652283498 1105 6 -0.0023120106805691876 1105 7 0.024152684758185217 1105 8 -0.08265669790558405 1105 9 -0.0314699951502746 1105 10-11 0.051379514280270655 1105 12-13 0.08775108161026424 1105 14-15 0.12026206763037806 1105 16-17 0.11468969929391903 1105 18-19 0.1235869601720836 1105 20-21 0.11290430422766917 1105 22-23 0.09035677665758612 1105 24-25 0.06727538448220827 1105 26-27 0.1035420452226603 1105 28-29 0.12465366022436086 1105 30-31 0.1295129498480847 1105 32-33 0.142340746518677 1105 34-35 0.13891455882940562 1105 36-37 0.11869547739243558 1105 38-39 0.10968344754932957 1105 40-41 0.13266308949592087 1105 42-43 0.11357597218017901 1105 44-45 0.1268947337638977 1105 46-47 0.14153778608637424 1105 48-49 0.1285885594539451 1105 50-51 0.0974370996589613 1105 52-53 -0.04655847274803193 1105 54-55 -0.0031334525923512047 1105 56-57 0.032189488189629856 1105 58-59 0.03089812534465608 1105 60-61 0.062445795484556754 1105 62-63 0.02879306684898353 1105 64-65 0.03709187471818609 1105 66-67 0.05742086569472349 1105 68-69 0.048627799189610244 1105 70-71 0.05326454329851771 1105 72-73 0.014406354867084303 1105 74-75 0.005229681908438977 1105 76-77 0.010813157383779526 1105 78-79 0.021385915250760945 1105 80-81 0.04594880282990488 1105 82-83 0.0065959817492782236 1105 84-85 -0.004953842120578145 1105 86-87 0.01511518177800042 1105 88-89 0.042649095098603595 1105 90-91 0.02761234753973696 1105 92-93 0.041239092228053664 1105 94-95 0.017061728705613177 1105 96-97 0.032220263153405426 1105 98-99 0.010249241827196443 1105 100 -0.032761555523521224 1106 1 0.1056431518449017 1106 2 0.11026401265320374 1106 3 0.08848615572512131 1106 4 0.049768555566004125 1106 5 0.060062687999014486 1106 6 0.03636180877553841 1106 7 0.057069893626092494 1106 8 -0.0524627066941008 1106 9 -0.056416713744724234 1106 10-11 0.04393294665285907 1106 12-13 0.1004739002098276 1106 14-15 0.12463736443400109 1106 16-17 0.12771256638986017 1106 18-19 0.11311911761245597 1106 20-21 0.12043228433107345 1106 22-23 0.1000289843628579 1106 24-25 0.13332808469516522 1106 26-27 0.1505883986435066 1106 28-29 0.13656732398815308 1106 30-31 0.14290166512149938 1106 32-33 0.13667945850454544 1106 34-35 0.11064236368913782 1106 36-37 0.1412846436407449 1106 38-39 0.1369973779416256 1106 40-41 0.13274671263312143 1106 42-43 0.14321878946959288 1106 44-45 0.15613052931058036 1106 46-47 0.1420190072101164 1106 48-49 0.14495816445511167 1106 50-51 0.14969257553588733 1106 52-53 0.09428613475435554 1106 54-55 0.09619445763139822 1106 56-57 0.13599192708536378 1106 58-59 0.13461134943068487 1106 60-61 0.18366214517734392 1106 62-63 0.10331995893319856 1106 64-65 0.08104938987320054 1106 66-67 0.08869346046077453 1106 68-69 0.08534858072987817 1106 70-71 0.031473060017169985 1106 72-73 0.04806571196616005 1106 74-75 0.07451744912516745 1106 76-77 0.08011025082753775 1106 78-79 0.07513339645831252 1106 80-81 0.0671563383238194 1106 82-83 0.0738794594704899 1106 84-85 0.08318197797905924 1106 86-87 0.06727095033185293 1106 88-89 0.06400686209019568 1106 90-91 0.07691171016221432 1106 92-93 0.06543002069845727 1106 94-95 0.05298036125559946 1106 96-97 0.043539120881995075 1106 98-99 0.09229777868237221 1106 100 0.058003269052790074 1107 1 0.035350790614451455 1107 2 0.04195742360583665 1107 3 0.04011247324817191 1107 4 -0.006913756686962813 1107 5 0.01942175599466367 1107 6 -0.007188772653115905 1107 7 -0.03420849756368938 1107 8 -0.0822059687093386 1107 9 -0.12079311003274995 1107 10-11 -0.0245534592301766 1107 12-13 -0.0072135604076919435 1107 14-15 0.005441268312097236 1107 16-17 -0.0137712402493122 1107 18-19 0.004877796079540531 1107 20-21 -0.040109332212438176 1107 22-23 -0.006312758920635986 1107 24-25 0.02326281718070078 1107 26-27 0.026362801461857543 1107 28-29 0.03940957566369718 1107 30-31 0.02741235567543754 1107 32-33 0.02570221799931005 1107 34-35 0.031028168810287582 1107 36-37 0.05171205098450571 1107 38-39 0.01342302381434024 1107 40-41 -0.01747033854978497 1107 42-43 -0.028270799234078936 1107 44-45 -0.01234987969440482 1107 46-47 0.017313838796297887 1107 48-49 0.011335727896707226 1107 50-51 -0.004327480888463242 1107 52-53 -0.0716159747146321 1107 54-55 -0.004092228941317444 1107 56-57 -0.006822201917330517 1107 58-59 0.036057402589875664 1107 60-61 0.06274145433700085 1107 62-63 -0.020983776674292187 1107 64-65 -0.01363549656203844 1107 66-67 -0.02544529834658249 1107 68-69 0.024658653336850023 1107 70-71 0.016807291987156248 1107 72-73 -0.0039632914422185195 1107 74-75 -0.012840147397717772 1107 76-77 0.004833230864200289 1107 78-79 0.010506026348899411 1107 80-81 -0.0015007548193324283 1107 82-83 -0.004995329296193063 1107 84-85 -0.026714213376841656 1107 86-87 -0.04294200149263361 1107 88-89 -0.030871446038347017 1107 90-91 -0.06786295043239221 1107 92-93 -0.048265953695775465 1107 94-95 -0.044592872261041805 1107 96-97 -0.012026697987224111 1107 98-99 -0.0014887396197451608 1107 100 -0.015361104837339212 1108 1 0.08262442095722378 1108 2 0.06222276481854294 1108 3 0.06183781010317091 1108 4 0.030269804608494155 1108 5 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-0.21921025337636735 2214 22-23 -0.18195567645268085 2214 24-25 -0.18890327621710412 2214 26-27 -0.22085991604298982 2214 28-29 -0.19547767217687095 2214 30-31 -0.18496105566693188 2214 32-33 -0.1969092628159359 2214 34-35 -0.180523195110176 2214 36-37 -0.20865246254010827 2214 38-39 -0.20462322945452627 2214 40-41 -0.17720594795265754 2214 42-43 -0.1744381276553426 2214 44-45 -0.1542973704239543 2214 46-47 -0.19294653888319147 2214 48-49 -0.1646617042113263 2214 50-51 -0.19733301943101367 2214 52-53 -0.1493773220553578 2214 54-55 -0.14954416347251254 2214 56-57 -0.15698235161477925 2214 58-59 -0.1754780279577659 2214 60-61 -0.11889739241129149 2214 62-63 -0.1335827622333241 2214 64-65 -0.10673744388743955 2214 66-67 -0.10212866809328602 2214 68-69 -0.10098561939297923 2214 70-71 -0.10811646329040059 2214 72-73 -0.10109278327558258 2214 74-75 -0.07035580028854582 2214 76-77 -0.061078029727248406 2214 78-79 -0.08714270904371091 2214 80-81 -0.10309661661775493 2214 82-83 -0.09248177227042476 2214 84-85 -0.09917329738998859 2214 86-87 -0.09205008180661878 2214 88-89 -0.0968146966022303 2214 90-91 -0.06679188690071669 2214 92-93 -0.05386278102214703 2214 94-95 -0.03511567924220316 2214 96-97 -0.04263916709916771 2214 98-99 -0.015832724356386052 2214 100 0.004676726797733011 2215 1 -0.2128607374666629 2215 2 -0.20403303820733498 2215 3 -0.20083459988402552 2215 4 -0.12499966507780869 2215 5 -0.15624209669637423 2215 6 -0.1019288090193271 2215 7 -0.11553587536887733 2215 8 0.09161551156051218 2215 9 0.06589115444027982 2215 10-11 -0.06301204907904179 2215 12-13 -0.1726422128957097 2215 14-15 -0.25827689743236704 2215 16-17 -0.27376248059010777 2215 18-19 -0.315450548797088 2215 20-21 -0.29102689291261186 2215 22-23 -0.27939415578907045 2215 24-25 -0.29436326883165975 2215 26-27 -0.3253000283142171 2215 28-29 -0.3154225532728674 2215 30-31 -0.3071255749643882 2215 32-33 -0.2853704952607288 2215 34-35 -0.3003127985421017 2215 36-37 -0.31087216909004667 2215 38-39 -0.3133272269633238 2215 40-41 -0.30345942183421926 2215 42-43 -0.3421389594978166 2215 44-45 -0.35217513440990444 2215 46-47 -0.3599081370876007 2215 48-49 -0.34703308141831 2215 50-51 -0.325975192041426 2215 52-53 -0.2770310019616531 2215 54-55 -0.30651933041899326 2215 56-57 -0.2878103131216818 2215 58-59 -0.33130678023417914 2215 60-61 -0.2701832004464748 2215 62-63 -0.25028967551072157 2215 64-65 -0.26793280010341647 2215 66-67 -0.29271718537597025 2215 68-69 -0.21293331142669558 2215 70-71 -0.21156167938919168 2215 72-73 -0.22834026928120466 2215 74-75 -0.23730935914295515 2215 76-77 -0.1950810795709117 2215 78-79 -0.18475144332519733 2215 80-81 -0.22475562494920354 2215 82-83 -0.2214670968319794 2215 84-85 -0.25300441293276066 2215 86-87 -0.21394481508446006 2215 88-89 -0.2406765677288405 2215 90-91 -0.20291091943267503 2215 92-93 -0.1960646053685906 2215 94-95 -0.20681341146986654 2215 96-97 -0.22315925735636455 2215 98-99 -0.17037925801332676 2215 100 -0.12846118366304893 2216 1 -0.21778344177407405 2216 2 -0.20649961031703157 2216 3 -0.22348291065818415 2216 4 -0.1353195521113193 2216 5 -0.1473506512890097 2216 6 -0.06906992590170802 2216 7 -0.09685629124923878 2216 8 0.11740217048388502 2216 9 0.0950749759899665 2216 10-11 -0.08177106134491652 2216 12-13 -0.20096190008714387 2216 14-15 -0.2855306632142671 2216 16-17 -0.2982798080064839 2216 18-19 -0.3348919627412883 2216 20-21 -0.2938636691709746 2216 22-23 -0.22167652257551396 2216 24-25 -0.17494008707213737 2216 26-27 -0.2019516511757331 2216 28-29 -0.19004477508052986 2216 30-31 -0.18715376231398295 2216 32-33 -0.18826423497236533 2216 34-35 -0.19096675161507193 2216 36-37 -0.17569727538823088 2216 38-39 -0.18121635456164142 2216 40-41 -0.18588250105189275 2216 42-43 -0.18364535748818867 2216 44-45 -0.20595583430829123 2216 46-47 -0.17502637295997658 2216 48-49 -0.20864404134892567 2216 50-51 -0.24730646324278638 2216 52-53 -0.22290400611782957 2216 54-55 -0.22661889218001363 2216 56-57 -0.2368633879295814 2216 58-59 -0.23438894188738857 2216 60-61 -0.20685083131058946 2216 62-63 -0.1996459029512323 2216 64-65 -0.21056995364727982 2216 66-67 -0.2085872713507797 2216 68-69 -0.2171487338820981 2216 70-71 -0.18233413361438977 2216 72-73 -0.1960629453911622 2216 74-75 -0.17192885530607072 2216 76-77 -0.1572509720410551 2216 78-79 -0.15962752379881806 2216 80-81 -0.19290087295365765 2216 82-83 -0.21949459422700812 2216 84-85 -0.1938803807733791 2216 86-87 -0.17836126765106997 2216 88-89 -0.20380351679013842 2216 90-91 -0.2262054574560466 2216 92-93 -0.21589103620867434 2216 94-95 -0.1988775935888185 2216 96-97 -0.2135156533336584 2216 98-99 -0.20781752937687514 2216 100 -0.15525606692165184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 64.0 8 208.0 9 432.0 10 745.0 11 1239.0 12 1845.0 13 2610.0 14 3537.0 15 4549.0 16 5881.0 17 7603.0 18 9792.0 19 12822.0 20 16586.0 21 21749.0 22 27567.0 23 33539.0 24 40480.0 25 49013.0 26 58659.0 27 71305.0 28 86361.0 29 107790.0 30 129418.0 31 162218.0 32 208215.0 33 290515.0 34 417388.0 35 1619475.0 36 1092259.0 37 2463563.0 38 3115486.0 39 1010892.0 40 11344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.073582068559723 22.908965620898748 33.88865818142352 24.12879412911801 2 14.726797035153158 25.731928972713984 37.516486105843775 22.024787886289083 3 12.510775687091005 21.97405716120861 41.24449488233798 24.270672269362407 4 12.828021688690624 21.005413692476754 43.94947761643084 22.217087002401787 5 11.064816560801717 20.949242386376003 46.16988784709108 21.816053205731198 6 10.21301073376198 17.42385742952848 44.16189245199389 28.20123938471565 7 12.869115371625034 19.055183005158614 41.213715427627875 26.86198619558848 8 5.286991103053887 19.52291678093363 45.630064432134084 29.5600276838784 9 8.858344928423174 17.209227261836375 45.338782905277256 28.593644904463194 10-11 13.71127073128669 32.543847568200725 41.402206438240974 12.342675262271614 12-13 12.183341233206605 33.04027419747422 42.058209586193996 12.718174983125186 14-15 12.168056340836168 32.03058267984329 42.294878251371834 13.506482727948702 16-17 12.625918776536697 32.084915258487904 42.64152888890172 12.647637076073678 18-19 12.699875044479572 31.25785144253194 42.44825586170753 13.594017651280954 20-21 11.891284427774378 30.9630090376044 43.31842664989336 13.827279884727863 22-23 11.955126951717657 30.91332770327338 43.86648449485007 13.2650608501589 24-25 11.886582116329446 30.691014637875803 43.82167524235771 13.600728003437043 26-27 12.467803505203289 30.55395314520562 43.36574825662039 13.612495092970697 28-29 12.187012205798894 31.17633442090137 43.696757332017924 12.939896041281818 30-31 12.141085977704204 30.793083699472827 43.86135319311665 13.204477129706325 32-33 12.15156662603038 30.923221325764132 43.9533914564385 12.971820591766987 34-35 12.22887882755057 31.147143825255558 43.76929652745501 12.854680819738858 36-37 12.295063037576268 30.8515970376331 43.74439729888575 13.108942625904879 38-39 12.27283236931645 30.951599547534375 43.80588668999961 12.96968139314956 40-41 12.359915611414678 31.257578552059996 43.64006941359423 12.742436422931094 42-43 12.476202401193957 31.306264451763944 43.565469377415845 12.652063769626254 44-45 12.463728867502603 31.129162285969812 43.61616115052567 12.790947696001915 46-47 12.530330564417891 31.211450385432432 43.56422804155092 12.69399100859876 48-49 12.446540310016923 30.97497637482804 43.74656098190143 12.831922333253601 50-51 12.326222306044741 31.038273753419514 43.85033679657703 12.78516714395871 52-53 12.453756126725214 31.042361686886977 43.82426324190482 12.679618944482987 54-55 12.418555987337562 30.915504450175273 43.84251013683361 12.82342942565355 56-57 12.342384700814305 30.922868896817622 43.85035677044735 12.884389631920717 58-59 12.375336651088856 31.053130802691125 43.78306492284308 12.788467623376937 60-61 12.378288129665052 30.90475388425632 43.8814533532443 12.835504632834335 62-63 12.2170349446294 30.84055595662235 44.016441538197604 12.925967560550648 64-65 12.401271024816202 30.882005236311862 43.76584974577474 12.9508739930972 66-67 12.363289134356553 30.80462330716908 43.8915166223919 12.940570936082466 68-69 12.340758167519114 30.84422374980894 43.964798836703395 12.85021924596855 70-71 12.330776825757932 30.923195688905903 43.92232527391185 12.823702211424317 72-73 12.38812741017509 30.731731435589854 44.07993645316601 12.800204701069044 74-75 12.362588060978922 30.63403724372305 44.13450113688335 12.868873558414673 76-77 12.47433469141315 30.624508900179105 44.04596870260145 12.855187705806296 78-79 12.562420413169642 30.61552742753846 44.034164204015305 12.787887955276595 80-81 12.478697841742196 30.511759765789943 44.18659048536079 12.822951907107072 82-83 12.563741831720932 30.490319145164058 44.14984440492451 12.796094618190498 84-85 12.621241976354455 30.37240217354185 44.171509383462556 12.834846466641137 86-87 12.588019790090502 30.515420396871846 44.12065650468357 12.775903308354074 88-89 12.679293042182884 30.770338403458453 43.88194261152051 12.668425942838155 90-91 12.710643474115521 30.8280053997626 43.8627455986639 12.598605527457982 92-93 12.683481500992208 30.879610080059116 43.80857337227854 12.62833504667014 94-95 12.790491374525956 30.966818430477193 43.64036645797395 12.602323737022905 96-97 12.778599595958356 31.155159803765635 43.60830947644824 12.457931123827766 98-99 12.681830763190296 31.41194661810749 43.522663053598414 12.383559565103793 100 12.81559564494069 31.554440045759158 43.27776133784189 12.352202971458267 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104426.0 1 274016.5 2 496954.5 3 402240.5 4 157233.5 5 41366.0 6 18397.0 7 13667.0 8 13772.5 9 16865.5 10 22796.5 11 32173.5 12 46057.5 13 64887.0 14 88803.5 15 116030.5 16 145138.0 17 175836.5 18 205926.5 19 233076.0 20 257931.5 21 283328.5 22 311320.0 23 340916.5 24 367504.5 25 386969.0 26 401662.0 27 414283.0 28 423277.0 29 426093.0 30 420783.5 31 407318.5 32 386377.0 33 361099.0 34 334448.5 35 307631.0 36 281476.0 37 255927.5 38 230924.5 39 207096.0 40 183500.0 41 161216.0 42 141592.0 43 123449.5 44 108332.0 45 96955.0 46 88512.0 47 82445.0 48 77819.5 49 74537.5 50 72723.5 51 72274.5 52 72116.5 53 70101.0 54 64523.0 55 55397.5 56 43649.0 57 30665.5 58 19081.5 59 10467.5 60 5176.5 61 2559.5 62 1333.5 63 764.5 64 491.0 65 344.0 66 263.0 67 212.5 68 171.0 69 131.0 70 109.0 71 81.5 72 61.0 73 56.0 74 45.0 75 31.5 76 27.0 77 22.0 78 14.0 79 10.5 80 11.5 81 10.5 82 12.5 83 8.5 84 4.5 85 5.5 86 4.0 87 3.0 88 2.0 89 1.5 90 1.0 91 1.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008019736671179611 2 5.412645672337195E-4 3 1.082529134467439E-4 4 0.004339137613990318 5 0.013838330768942095 6 0.007045460450158915 7 0.0 8 0.0 9 0.0 10-11 0.01146127721117401 12-13 0.005286350606649327 14-15 0.001078018596407158 16-17 2.7063228361685974E-4 18-19 6.765807090421494E-5 20-21 7.171755515846783E-4 22-23 1.1276345150702489E-4 24-25 3.5633250676219867E-4 26-27 0.0026025804607821344 28-29 0.0015786883210983486 30-31 0.002142505578633473 32-33 0.002088379121910101 34-35 0.0023093954868638696 36-37 8.750443836945132E-4 38-39 0.0010013394493823809 40-41 3.1573766421966965E-5 42-43 0.0 44-45 5.638172575351245E-4 46-47 4.961591866309095E-5 48-49 4.285011157266946E-4 50-51 0.03190754623842776 52-53 0.03689169079503826 54-55 7.487493180066454E-4 56-57 1.1727398956730589E-4 58-59 4.285011157266946E-4 60-61 0.004230884700543574 62-63 0.013689483012952822 64-65 0.017767009419446842 66-67 0.01885404909197456 68-69 0.019539650877137275 70-71 0.01743774014104633 72-73 0.01509226034970021 74-75 0.016066536570720907 76-77 0.014532953630225366 78-79 0.015683140835597024 80-81 0.0159492625811536 82-83 0.014208194889885138 84-85 0.014284874036909914 86-87 0.012836991319559713 88-89 0.011163581699195464 90-91 0.008669254151860074 92-93 0.00929621894223913 94-95 0.010396790228947695 96-97 0.005872720554485856 98-99 0.00851589585781052 100 0.009309750556419975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1085152E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.08271972164418 #Duplication Level Percentage of deduplicated Percentage of total 1 88.54185915267583 67.3650545354633 2 9.668358806249934 14.711900664484082 3 1.2612108849429 2.8786906280699243 4 0.2178102004299899 0.6628636972732025 5 0.07556781145669507 0.28747023095188917 6 0.0451813854159646 0.206251360994305 7 0.02966432053600317 0.157985952955162 8 0.02397679235238753 0.14593756578966097 9 0.01710956456027736 0.11715679844990627 >10 0.1032136245772804 1.4677920389817678 >50 0.007126685817281003 0.36606671042244776 >100 0.005047973525439813 0.8407083433551834 >500 0.001118033341655705 0.5989413988298955 >1k 0.0015554933419170767 3.093636880279064 >5k 0.0010805310954954527 6.061550312127864 >10k+ 1.1873968082367611E-4 1.037992881572396 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16898 0.15243814428525654 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11745 0.10595253903600058 No Hit CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11402 0.10285830992664782 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11208 0.1011082211592588 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11145 0.1005398933636634 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.683277955954055E-4 0.0 0.0 1.804215224112398E-5 0.0 2 0.0013531614180842988 0.0 0.0 2.7063228361685974E-5 0.0 3 0.0023815640958283657 0.0 0.0 8.118968508505792E-5 0.0 4 0.004059484254252896 0.0 0.0 8.118968508505792E-5 0.0 5 0.0075325985606692625 0.0 0.0 8.118968508505792E-5 0.0 6 0.013883436149544905 0.0 0.0 8.118968508505792E-5 0.0 7 0.02025231589066167 0.0 0.0 8.118968508505792E-5 0.0 8 0.03838467889299128 0.0 0.0 8.118968508505792E-5 0.0 9 0.10857767218708413 0.0 0.0 9.021076120561992E-5 0.0 10-11 0.25243226254362594 0.0 0.0 9.021076120561992E-5 0.0 12-13 0.40900205969209985 0.0 0.0 9.021076120561992E-5 0.0 14-15 0.5761986845105957 0.0 0.0 9.472129926590091E-5 0.0 16-17 0.7578064784316896 0.0 0.0 1.127634515070249E-4 0.0 18-19 0.9509296760206807 0.0 0.0 1.4433721792899185E-4 0.0 20-21 1.2029469690627608 0.0 0.0 1.4433721792899185E-4 0.0 22-23 1.4883602859031613 0.0 0.0 1.4884775598927286E-4 0.0 24-25 1.776921958309638 0.0 0.0 1.5335829404955386E-4 0.0 26-27 2.0437247951133193 0.0 0.0 1.5335829404955386E-4 0.0 28-29 2.3026026165450864 0.0 0.0 1.5335829404955386E-4 0.0 30-31 2.5801089601658145 0.0 0.0 1.5786883210983484E-4 0.0 32-33 2.897253912260292 0.0 0.0 1.6237937017011584E-4 0.0 34-35 3.2137448363360286 0.0 0.0 1.6237937017011584E-4 0.0 36-37 3.4511750492911597 0.0 0.0 1.7140044629067783E-4 0.0 38-39 3.625674235229251 0.0 0.0 2.3003744107433078E-4 0.0 40-41 3.7836017043338694 0.0 0.0 2.525901313757357E-4 0.0 42-43 3.9250521779042815 0.0 0.0 3.0671658809910767E-4 0.0 44-45 4.051130737765256 0.0 0.0 3.428008925813557E-4 0.0 46-47 4.171088497478429 0.0 0.0 3.6986412094304163E-4 0.0 48-49 4.2889578780696915 0.0 0.0 4.375221918472566E-4 0.0 50-51 4.414215520003695 0.0 0.0 4.690959582692235E-4 0.0 52-53 4.549157287153122 0.0 0.0 5.142013388720335E-4 0.0 54-55 4.679809532607221 0.0 0.0 5.863699478365295E-4 0.0 56-57 4.807935876747563 0.0 0.0 6.089226381379344E-4 0.0 58-59 4.939052707621871 0.0 0.0 6.314753284393394E-4 0.0 60-61 5.072406765374079 0.0 0.0 6.765807090421494E-4 0.0 62-63 5.210785562525439 0.0 0.0 6.856017851627113E-4 0.0 64-65 5.350526542171005 0.0 0.0 7.667914702477693E-4 0.0 66-67 5.494056373787206 0.0 0.0 8.615127695136703E-4 0.0 68-69 5.639169404262567 0.0 0.0 9.066181501164801E-4 0.0 70-71 5.787466874608485 0.0 0.0 9.246603023576041E-4 0.0 72-73 5.9421828406141834 0.0 0.0 9.47212992659009E-4 0.0 74-75 6.103660103172244 0.0 0.0 0.001010360525502943 0.0 76-77 6.270689838082509 0.0 0.0 0.0010689975202865958 0.0 78-79 6.439618509516153 0.0 0.0 0.0012945244233006459 0.0 80-81 6.618411727687631 0.0 0.0 0.0014749459457118856 0.0 82-83 6.804769118186201 0.0 0.0 0.0015200513263146954 0.0 84-85 6.998632044017079 0.0 0.0 0.0015516250927366626 0.0 86-87 7.197912126058353 0.0 0.0 0.0015516250927366626 0.0 88 7.351599689386307 0.0 0.0 0.0015516250927366626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGGC 480 0.0 70.50501 1 GCTTTCT 1800 0.0 56.92627 1 AGGCATA 660 0.0 50.561695 4 GCTTTTC 1975 0.0 48.788284 1 GGCTGCC 60 1.6056183E-6 47.00334 1 GCCTTTT 2070 0.0 46.77627 1 GGCTCTA 585 0.0 44.9979 6 GGCATAT 755 0.0 44.207603 5 GCATATC 755 0.0 43.582405 6 AGGACCC 175 0.0 42.97274 2 GCCGCGT 220 0.0 42.735905 5 CCGCGCT 225 0.0 41.778862 3 CTGCCGT 175 0.0 40.293854 5 CTAGCTT 490 0.0 40.288578 1 GCCCTAC 3360 0.0 40.288403 7 CCGGTCC 130 5.456968E-12 39.77206 1 TGCTCTA 1315 0.0 39.321224 6 CCCGTGA 60 8.821572E-5 39.172283 6 GTGTCCC 120 9.822543E-11 39.169453 1 TTAGGCA 850 0.0 38.70706 2 >>END_MODULE