FastQCFastQC Report
Thu 26 May 2016
SRR1248446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42830232
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22920495.351474631283809No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5066851.1830078342793007No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4029290.9407583876734547No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3875100.9047581157160204No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2224190.5193037478760331No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1595680.37255927075062306No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1413060.32992116409736No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1365140.3187328053698145No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1187590.2772784420126419No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110260.25922343824801136No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA936390.21862828107024965No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA669730.1563685202545716No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA572380.133639248089994No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA565290.1319838753149878No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA547300.12778357119335707No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA535430.125012164304877No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA528390.12336846552687362No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA517830.1209029173598686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN466940.10902112321035291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCTG59300.036.5373578
GCCTGCT19850.035.987967
TTGCTGA105950.035.355419
CCGTTTT42600.035.312941
CTGCTGA87100.034.589039
GCTTGCT20100.033.903627
TACCCGA138350.033.0859646
CTTGCTG62350.032.4886558
CCCGACG153350.032.4872558
CTACCCG133650.031.2997885
ACCCGAC156550.030.891327
CCTGTTT28200.030.173311
CCTTTTG30150.028.5336461
TTGCGCC10600.028.3797593
CCTTGTT19850.028.1825261
TTGCCAA24700.027.7828165
GACACGC15200.026.9041562
TTTTGCC31100.026.7514573
GTTTGCC11950.026.7470323
CATGCCA21200.026.6052474
Produced by FastQC (version 0.11.5)