##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248446_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42830232
Sequences flagged as poor quality	0
Sequence length	100
%GC	27
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.420168048587737	33.0	31.0	34.0	25.0	34.0
2	30.828108776062667	33.0	31.0	34.0	26.0	34.0
3	31.167337010922566	33.0	31.0	34.0	27.0	34.0
4	34.554232977304444	37.0	35.0	37.0	31.0	37.0
5	34.42898317711658	37.0	35.0	37.0	30.0	37.0
6	34.23838740822137	37.0	35.0	37.0	30.0	37.0
7	34.40768733169598	37.0	35.0	37.0	30.0	37.0
8	34.42692948756383	37.0	35.0	37.0	30.0	37.0
9	35.85984294925136	39.0	37.0	39.0	30.0	39.0
10-11	36.34842585022654	39.0	37.0	39.0	31.0	39.0
12-13	36.416919980727634	39.0	37.0	39.0	31.5	39.0
14-15	37.68781915540406	40.0	38.0	41.0	31.5	41.0
16-17	37.5692442665265	40.0	38.0	41.0	31.0	41.0
18-19	37.19461380690163	40.0	36.5	41.0	31.0	41.0
20-21	36.934210302666585	40.0	35.0	41.0	30.0	41.0
22-23	36.73105376828218	39.0	35.0	41.0	30.0	41.0
24-25	36.5930158048175	39.0	35.0	41.0	30.0	41.0
26-27	36.47695238961116	39.0	35.0	41.0	28.5	41.0
28-29	36.36230492517528	39.0	35.0	41.0	27.5	41.0
30-31	36.2846990882515	39.0	35.0	41.0	27.0	41.0
32-33	36.20650998341545	39.0	35.0	41.0	27.0	41.0
34-35	36.11124712095886	39.0	35.0	41.0	26.0	41.0
36-37	35.999162927718906	39.0	35.0	41.0	25.5	41.0
38-39	35.88713750137987	39.0	35.0	41.0	25.0	41.0
40-41	35.764391738527124	39.0	35.0	41.0	25.0	41.0
42-43	35.63520173087085	38.0	35.0	40.0	25.0	41.0
44-45	35.47947998507223	38.0	35.0	40.0	25.0	41.0
46-47	35.32179796504488	38.0	34.5	40.0	25.0	41.0
48-49	35.1324372233146	38.0	34.0	40.0	25.0	41.0
50-51	34.90287971122827	37.0	34.0	40.0	25.0	41.0
52-53	34.647731175026095	37.0	33.0	40.0	25.0	41.0
54-55	34.41565561680824	36.0	33.0	40.0	25.0	41.0
56-57	34.16120794069012	35.5	33.0	39.5	25.0	41.0
58-59	34.22599842559807	35.0	33.0	39.0	25.5	41.0
60-61	34.0472243881378	35.0	33.0	39.0	25.5	41.0
62-63	33.81151589372665	35.0	33.0	39.0	25.0	41.0
64-65	33.57634523203143	35.0	32.5	39.0	25.0	40.5
66-67	33.348891292487046	35.0	32.0	38.0	25.0	40.0
68-69	33.12593258892457	35.0	32.0	37.0	25.0	40.0
70-71	32.91397318370818	35.0	32.0	37.0	25.0	40.0
72-73	32.85339150159167	35.0	32.0	36.0	25.0	39.0
74-75	32.857481824987545	35.0	32.0	36.0	25.0	39.0
76-77	32.69634786241644	35.0	32.0	36.0	25.0	39.0
78-79	32.478612840574854	35.0	32.0	35.5	25.0	38.5
80-81	32.26307449140131	35.0	32.0	35.0	24.5	37.0
82-83	32.046472769514764	35.0	32.0	35.0	24.0	37.0
84-85	31.83585371426426	35.0	31.5	35.0	24.0	36.5
86-87	31.630316851890974	35.0	31.0	35.0	23.0	36.0
88-89	31.43017364463494	35.0	31.0	35.0	23.0	36.0
90-91	31.222089749595565	35.0	31.0	35.0	20.5	36.0
92-93	31.03034807282856	35.0	31.0	35.0	20.0	36.0
94-95	30.833364689689283	35.0	30.0	35.0	19.5	35.0
96-97	30.640284017140043	35.0	30.0	35.0	18.5	35.0
98-99	30.461892116297665	35.0	30.0	35.0	17.5	35.0
100	29.645537339139327	34.0	28.0	35.0	14.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2949634942625572
1101	2	0.2642016760358068
1101	3	0.26430272783389697
1101	4	0.2398102911984168
1101	5	0.144159720823815
1101	6	0.18497118006225577
1101	7	0.18574924279558758
1101	8	0.23839451606297501
1101	9	0.3169259876812305
1101	10-11	0.35719354993656793
1101	12-13	0.33254504212879255
1101	14-15	0.4829178632483888
1101	16-17	0.48013180497520835
1101	18-19	0.4889868833999529
1101	20-21	0.5050668332847863
1101	22-23	0.4945779766823435
1101	24-25	0.5120804822650129
1101	26-27	0.5192263966216046
1101	28-29	0.5495639844762295
1101	30-31	0.5467280133532384
1101	32-33	0.5877913104784795
1101	34-35	0.574766057754168
1101	36-37	0.5808947772875257
1101	38-39	0.568313235682659
1101	40-41	0.5849613135287797
1101	42-43	0.5847392838283128
1101	44-45	0.5724556660080609
1101	46-47	0.5924461816313737
1101	48-49	0.565394355466438
1101	50-51	0.5927850328322677
1101	52-53	0.5738216340068476
1101	54-55	0.5485187797019506
1101	56-57	0.5420523701869584
1101	58-59	0.5384887166735624
1101	60-61	0.5002911376210122
1101	62-63	0.4768944870874421
1101	64-65	0.47233768892262873
1101	66-67	0.4493963674198369
1101	68-69	0.4563611177526212
1101	70-71	0.4318945243224732
1101	72-73	0.4445322341137796
1101	74-75	0.446411524578302
1101	76-77	0.4710423903811787
1101	78-79	0.44385976092756607
1101	80-81	0.4159252965635716
1101	82-83	0.38632276811066646
1101	84-85	0.41430763610654964
1101	86-87	0.40452463865592136
1101	88-89	0.428742606134378
1101	90-91	0.40754128923464705
1101	92-93	0.37294474309457115
1101	94-95	0.37087997027243347
1101	96-97	0.40560314913949824
1101	98-99	0.4147488497265357
1101	100	0.4070742789974773
1102	1	0.10626378595541297
1102	2	0.13287510203051411
1102	3	0.17007155269655172
1102	4	0.12612958318627676
1102	5	0.08469962236940631
1102	6	-0.06621170985063429
1102	7	0.007157565620381945
1102	8	0.09331245649561737
1102	9	0.14882455653446414
1102	10-11	0.18308597424842787
1102	12-13	0.20517664114718315
1102	14-15	0.3084889062497638
1102	16-17	0.3279429348504266
1102	18-19	0.3220116689732464
1102	20-21	0.304135389306019
1102	22-23	0.30791904777457546
1102	24-25	0.27261758694001514
1102	26-27	0.2991648983394555
1102	28-29	0.29261221264714266
1102	30-31	0.2974852955513114
1102	32-33	0.31699491470730834
1102	34-35	0.326034246858363
1102	36-37	0.3275205572050979
1102	38-39	0.30662178218077685
1102	40-41	0.2987636643523004
1102	42-43	0.3320807798569092
1102	44-45	0.3104531405876898
1102	46-47	0.3213218341687849
1102	48-49	0.3806287835923925
1102	50-51	0.31778867560919366
1102	52-53	0.3357912512322514
1102	54-55	0.31978120756794226
1102	56-57	0.3054561540068832
1102	58-59	0.3042302382133002
1102	60-61	0.2835048543390357
1102	62-63	0.27682579295972687
1102	64-65	0.2702869681489162
1102	66-67	0.2882319349218392
1102	68-69	0.2827469385555901
1102	70-71	0.2693327365364979
1102	72-73	0.2918784487953303
1102	74-75	0.2801828329533649
1102	76-77	0.2579526081827055
1102	78-79	0.23446755441652556
1102	80-81	0.24450911936674657
1102	82-83	0.25269387697206724
1102	84-85	0.20114009761584128
1102	86-87	0.18719859953434081
1102	88-89	0.18995680033145845
1102	90-91	0.2029800624790674
1102	92-93	0.19008266909339255
1102	94-95	0.21466350561120606
1102	96-97	0.27094819570272577
1102	98-99	0.2673910827506276
1102	100	0.2228206914957056
1103	1	0.05005729065917208
1103	2	0.10398179162195476
1103	3	0.13507499158910008
1103	4	0.09953852837555388
1103	5	0.06837538438516333
1103	6	0.013137013614802129
1103	7	0.03569008342302027
1103	8	0.07709959717492154
1103	9	0.10234494956666396
1103	10-11	0.17917196618689246
1103	12-13	0.1798849905507538
1103	14-15	0.2553683499738355
1103	16-17	0.29969058543266414
1103	18-19	0.25119163667444866
1103	20-21	0.2659157299804633
1103	22-23	0.2870060423913614
1103	24-25	0.2886739783683794
1103	26-27	0.27421200225620623
1103	28-29	0.29771877705447736
1103	30-31	0.26526536434462855
1103	32-33	0.30521233835524697
1103	34-35	0.2995245697103144
1103	36-37	0.2808381279310481
1103	38-39	0.2753994151370307
1103	40-41	0.28062232659892317
1103	42-43	0.2895638831184755
1103	44-45	0.2385342630720757
1103	46-47	0.2733830500965908
1103	48-49	0.2870189681939763
1103	50-51	0.2653088463841726
1103	52-53	0.24244616299222344
1103	54-55	0.23331681525106518
1103	56-57	0.2688776328601037
1103	58-59	0.2684931272462663
1103	60-61	0.2608853454645228
1103	62-63	0.2497322835991298
1103	64-65	0.24776675541992432
1103	66-67	0.2613596867472481
1103	68-69	0.22379961123483838
1103	70-71	0.22515007974396894
1103	72-73	0.25546440738541776
1103	74-75	0.24519527524946483
1103	76-77	0.24431137311700013
1103	78-79	0.1978273871241072
1103	80-81	0.23720074610942987
1103	82-83	0.23987729464931817
1103	84-85	0.214448978692225
1103	86-87	0.16954258858868698
1103	88-89	0.19012462505072492
1103	90-91	0.21031350579756847
1103	92-93	0.20079225267551237
1103	94-95	0.19868429644386865
1103	96-97	0.2181955382553511
1103	98-99	0.20523114332874215
1103	100	0.22355391223329235
1104	1	0.062047866161915266
1104	2	0.10112804890326643
1104	3	0.1640120901372164
1104	4	0.13128904547106401
1104	5	0.0961023468065747
1104	6	-1.4011132137170534E-4
1104	7	0.056971659530717034
1104	8	0.10995147559835772
1104	9	0.11248617421144047
1104	10-11	0.18401670175555296
1104	12-13	0.19182696800223908
1104	14-15	0.2343843773114358
1104	16-17	0.2715437633491149
1104	18-19	0.2617069930802245
1104	20-21	0.24757390807249635
1104	22-23	0.2552934182115365
1104	24-25	0.27602873465089317
1104	26-27	0.2494850384343934
1104	28-29	0.23878514869880263
1104	30-31	0.23766988168959813
1104	32-33	0.26653294234794345
1104	34-35	0.2678483323361718
1104	36-37	0.2847324548157033
1104	38-39	0.25711277892595064
1104	40-41	0.28304008472262154
1104	42-43	0.3005003998024307
1104	44-45	0.281071643132357
1104	46-47	0.29887728013422077
1104	48-49	0.2655001920238149
1104	50-51	0.2942493520727538
1104	52-53	0.2501389576381925
1104	54-55	0.2681745691914088
1104	56-57	0.26526855356834034
1104	58-59	0.26989833886651127
1104	60-61	0.2514608693494509
1104	62-63	0.2519107342902771
1104	64-65	0.25723555057637526
1104	66-67	0.24745404967841722
1104	68-69	0.22583739920146684
1104	70-71	0.24345345314149114
1104	72-73	0.2353660020313484
1104	74-75	0.24352776116970887
1104	76-77	0.24939906342444118
1104	78-79	0.26084991300245264
1104	80-81	0.23941918862980316
1104	82-83	0.2609380045039842
1104	84-85	0.21951100339696694
1104	86-87	0.22196268612994885
1104	88-89	0.222385478343206
1104	90-91	0.24921314481082746
1104	92-93	0.20592499858907587
1104	94-95	0.21669200548099354
1104	96-97	0.2707992619789579
1104	98-99	0.27446370236695117
1104	100	0.28192559581205856
1105	1	0.09302195958629866
1105	2	0.0837037788672248
1105	3	0.12838214986675212
1105	4	0.15361208239806956
1105	5	0.13066419962430587
1105	6	0.08457721630642112
1105	7	0.07075733737421785
1105	8	0.10380895910664378
1105	9	0.11264423214493746
1105	10-11	0.13339571697401453
1105	12-13	0.15732030535640718
1105	14-15	0.22864135552270426
1105	16-17	0.2523218538415435
1105	18-19	0.24656798092806298
1105	20-21	0.24536710138811202
1105	22-23	0.2046680907962397
1105	24-25	0.21283309845428988
1105	26-27	0.21413982808431342
1105	28-29	0.22036411839992098
1105	30-31	0.20420933017030052
1105	32-33	0.24230413637888404
1105	34-35	0.25813841256356795
1105	36-37	0.23563981181300164
1105	38-39	0.2100974889769276
1105	40-41	0.21104742245464791
1105	42-43	0.19799496687684126
1105	44-45	0.22355627371407394
1105	46-47	0.23454321546545742
1105	48-49	0.23567543175858674
1105	50-51	0.23612638776305062
1105	52-53	0.20149367665879936
1105	54-55	0.1972475402052467
1105	56-57	0.16578738116697167
1105	58-59	0.16938444662223162
1105	60-61	0.21036436034633255
1105	62-63	0.21007068626275327
1105	64-65	0.19437130382964796
1105	66-67	0.1854808800234835
1105	68-69	0.20362183823907998
1105	70-71	0.18551538426866188
1105	72-73	0.21963496710160513
1105	74-75	0.17637504233303503
1105	76-77	0.1933639015555073
1105	78-79	0.17585326706463889
1105	80-81	0.18275549718578787
1105	82-83	0.14833238476406052
1105	84-85	0.15052462253313337
1105	86-87	0.11867088597627173
1105	88-89	0.16686721905745827
1105	90-91	0.1641751962857363
1105	92-93	0.1419688038883038
1105	94-95	0.13088417967690447
1105	96-97	0.14308307547686638
1105	98-99	0.14279384462420452
1105	100	0.16816251638608293
1106	1	0.05814491327732796
1106	2	0.06128183637350304
1106	3	0.09872479532880618
1106	4	0.09041361388280933
1106	5	0.039956215967890785
1106	6	0.05114840531555842
1106	7	0.038003966981108306
1106	8	0.08342233659816145
1106	9	0.12460230170493247
1106	10-11	0.12254830183647414
1106	12-13	0.12030322954148431
1106	14-15	0.16828093381413822
1106	16-17	0.1498942281417186
1106	18-19	0.15650307607822356
1106	20-21	0.14752604408020886
1106	22-23	0.13204310593397395
1106	24-25	0.16708117291670277
1106	26-27	0.17563684688884962
1106	28-29	0.18957820357220356
1106	30-31	0.17895539020614848
1106	32-33	0.19151078868303273
1106	34-35	0.19230658484832475
1106	36-37	0.16605504052093778
1106	38-39	0.1687689404281869
1106	40-41	0.1796440746859318
1106	42-43	0.1887335980339202
1106	44-45	0.18786812353172166
1106	46-47	0.19688376269783703
1106	48-49	0.1803697457895197
1106	50-51	0.18879277205895306
1106	52-53	0.21965120309251063
1106	54-55	0.20298036023879718
1106	56-57	0.168698692199861
1106	58-59	0.18444825754502148
1106	60-61	0.15422616949843615
1106	62-63	0.15177627209146038
1106	64-65	0.17601163494843064
1106	66-67	0.1539247156346235
1106	68-69	0.17055893386147147
1106	70-71	0.17535282956787057
1106	72-73	0.16207568166211672
1106	74-75	0.16047511528759628
1106	76-77	0.15456111353818613
1106	78-79	0.1835515474787286
1106	80-81	0.15301884499898222
1106	82-83	0.1599309661155246
1106	84-85	0.1521268980370607
1106	86-87	0.1222711918019499
1106	88-89	0.09000191006851566
1106	90-91	0.12503328337061603
1106	92-93	0.1314988454625805
1106	94-95	0.1373029528393701
1106	96-97	0.14482805851163505
1106	98-99	0.15441971845437408
1106	100	0.09856067857417017
1107	1	0.001535559631967942
1107	2	0.028689430996443832
1107	3	0.07989632652797596
1107	4	0.041452732437065265
1107	5	0.04774761943333772
1107	6	0.037270540801010554
1107	7	0.029390151202612458
1107	8	0.04438415760409953
1107	9	0.05516772639379752
1107	10-11	0.05120711867581207
1107	12-13	0.08754456453521442
1107	14-15	0.1337195475091093
1107	16-17	0.10787028571671442
1107	18-19	0.11682172405433278
1107	20-21	0.10720703621981897
1107	22-23	0.10721430286908173
1107	24-25	0.10616967971620994
1107	26-27	0.1359711263197667
1107	28-29	0.11628238300847471
1107	30-31	0.12366037381025308
1107	32-33	0.13189520655026854
1107	34-35	0.15552160462079456
1107	36-37	0.14844505044976586
1107	38-39	0.11509754418929674
1107	40-41	0.11835222933490286
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2216	50-51	-0.1750425458395526
2216	52-53	-0.1809772552517046
2216	54-55	-0.21387338770168185
2216	56-57	-0.22520419617270448
2216	58-59	-0.24377356958060403
2216	60-61	-0.24188774142691472
2216	62-63	-0.2543655412853951
2216	64-65	-0.2752269634708071
2216	66-67	-0.2796357112975798
2216	68-69	-0.31476436266505203
2216	70-71	-0.3067462971830821
2216	72-73	-0.31010399288659585
2216	74-75	-0.30807548779339555
2216	76-77	-0.3112538744212614
2216	78-79	-0.3176665033148538
2216	80-81	-0.36135279427879397
2216	82-83	-0.343567695849039
2216	84-85	-0.34010179906120896
2216	86-87	-0.33553313700166143
2216	88-89	-0.31803088238330446
2216	90-91	-0.3285403890401035
2216	92-93	-0.39929743308128707
2216	94-95	-0.37195821396308304
2216	96-97	-0.3225702266939763
2216	98-99	-0.3101767858531197
2216	100	-0.24339370133864335
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	48828.0
1	2396.0
2	1402.0
3	1156.0
4	1037.0
5	2442.0
6	10850.0
7	27065.0
8	43681.0
9	64953.0
10	92747.0
11	122894.0
12	139490.0
13	149612.0
14	162281.0
15	178337.0
16	194703.0
17	208914.0
18	223246.0
19	237823.0
20	254267.0
21	272400.0
22	292509.0
23	326102.0
24	414708.0
25	538846.0
26	415700.0
27	788192.0
28	552334.0
29	879233.0
30	819830.0
31	1001856.0
32	1286896.0
33	2075459.0
34	2570213.0
35	7186827.0
36	6001730.0
37	7081602.0
38	6080276.0
39	2059457.0
40	17938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.854904083788334	29.912887329198508	29.276509239315846	24.955699347697315
2	13.14898088533217	31.767001103227493	32.20888833842735	22.875129673012985
3	12.07816875524889	33.659320965009556	30.79740303875134	23.465107240990204
4	10.687365894206541	33.02262186115957	31.64123617843314	24.648776066200753
5	10.657113873284679	32.625102412064976	32.23909476490777	24.478688949742573
6	10.488077508903544	30.246276548306135	33.33974339418332	25.925902548607
7	13.529342209299886	35.53466548886913	28.28635741174362	22.649634890087373
8	13.963868019586142	31.68819413668345	24.964762997714548	29.38317484601586
9	10.603210815771202	30.719992788413602	27.47698109920539	31.199815296609806
10-11	13.044418400381662	46.656877642082534	26.071451970007104	14.227251987528694
12-13	12.417450667949431	46.976842691252415	26.580214540652253	14.025492100145904
14-15	12.940914747897578	46.110501272944624	26.411402048728917	14.537181930428883
16-17	12.953983634967242	45.82822398993626	26.645556138960984	14.572236236135513
18-19	13.100304560754186	45.133750422676314	27.25025974318231	14.515685273387188
20-21	12.59739050467385	44.93224976603132	27.807505223123208	14.662854506171625
22-23	12.678166061573076	45.126408444328014	28.230046100774757	13.965379393324154
24-25	12.661878643212749	44.89497500465925	28.04098834197639	14.402158010151608
26-27	13.3951083506913	44.99929751343136	27.447879272662078	14.157714863215261
28-29	13.166654989672672	45.3292232496717	27.818849244925975	13.68527251572966
30-31	13.303894084638765	45.226186579378066	27.881727846621462	13.588191489361703
32-33	13.448532044700165	45.30752084440762	27.6913406203176	13.552606490574615
34-35	13.414511123367875	45.38796248794267	27.646857475714054	13.550668912975395
36-37	13.712776999657518	45.188636049977475	27.52161030034429	13.57697665002072
38-39	13.84221190602593	45.249457777744105	27.64534614186803	13.26298417436193
40-41	13.935479383353538	45.59107599827449	27.54242863873201	12.931015979639959
42-43	14.04398639971897	45.61383104392152	27.615349589998406	12.726832966361107
44-45	14.166129877375901	45.46963788764531	27.54765153993627	12.816580695042513
46-47	14.175775871066357	45.26883784685632	27.667862242437778	12.887524039639548
48-49	14.165507889580073	45.2481137877306	27.800577669476784	12.785800653212542
50-51	14.282093042799943	45.17414764088925	27.955963401592186	12.587795914718619
52-53	14.335277614259335	45.099384974777685	28.133506443369654	12.431830967593328
54-55	14.320437330092936	45.08397378699821	28.092596428348394	12.502992454560458
56-57	14.340511713163043	45.10248802127498	27.839791532501874	12.717208733060106
58-59	14.233001159508749	44.88992639556254	27.98826418675092	12.888808258177791
60-61	14.190198424695808	44.822191016417776	28.211183386468434	12.776427172417982
62-63	14.240730504898005	44.57440371219804	28.2521886965425	12.932677086361451
64-65	14.255055442528269	44.51531847170797	28.183184972479637	13.046441113284121
66-67	14.137703808171304	44.31947347082834	28.475230051727046	13.06759266927331
68-69	14.004311978252835	44.54149542981571	28.33321280484527	13.120979787086181
70-71	13.808004149851435	44.79692622991537	28.320301345791304	13.074768274441887
72-73	13.780281555933621	44.923216422657234	28.39822370858091	12.898278312828229
74-75	13.74884421136317	45.14784784303771	28.248256859224135	12.855051086374983
76-77	13.595332406727811	45.471762826389735	28.19862680180001	12.734277965082445
78-79	13.349272480320066	45.857996745026334	28.131490295243523	12.661240479410077
80-81	13.164921000337998	46.2394076649424	28.01922735403139	12.57644398068821
82-83	13.026739180244611	46.4621695779291	28.006884486282697	12.504206755543592
84-85	12.925753627255393	46.84111020739045	27.81552029006122	12.417615875292938
86-87	12.900154566861557	47.184586859336534	27.551644175447944	12.363614398353967
88-89	12.91768661848538	47.34659802700759	27.482413917827508	12.253301436679527
90-91	12.937011106003386	47.4909274197319	27.46071034359712	12.111351130667598
92-93	13.04620078663884	47.60622286362789	27.25247090633766	12.095105443395601
94-95	13.20308737182598	47.489359103775804	27.102183204518294	12.205370319879929
96-97	13.389166373341027	47.22184508971942	27.16265645035854	12.226332086581008
98-99	13.51842820927941	47.12538418513393	27.116184163293468	12.240003442293188
100	13.802110754775878	46.95646184861615	26.987938664371924	12.253488732236049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3188167.0
1	2667604.0
2	1275527.5
3	261295.0
4	118098.0
5	114590.5
6	104521.5
7	92910.0
8	89839.0
9	100953.0
10	130774.0
11	184796.5
12	264818.0
13	364299.5
14	477790.5
15	597273.5
16	705993.5
17	799951.5
18	883755.0
19	960733.0
20	1037453.5
21	1118856.5
22	1210686.5
23	1304592.0
24	1373223.0
25	1404069.0
26	1406714.0
27	1394685.5
28	1370267.0
29	1321990.0
30	1243840.5
31	1133904.5
32	1005669.5
33	880764.0
34	763694.0
35	660106.5
36	572560.0
37	500737.5
38	443866.0
39	397913.5
40	363034.0
41	340030.5
42	331981.0
43	341556.0
44	367760.5
45	406729.0
46	451565.0
47	495875.5
48	536926.5
49	575604.0
50	619092.0
51	673670.0
52	731035.5
53	772937.5
54	774528.5
55	715905.5
56	601102.0
57	456821.0
58	314065.0
59	196496.5
60	114847.0
61	65134.0
62	38445.0
63	25080.5
64	18290.5
65	14630.5
66	12260.0
67	10509.0
68	9291.5
69	8325.0
70	7383.5
71	6555.0
72	5999.5
73	5503.0
74	5081.5
75	4703.0
76	4369.0
77	4230.0
78	4058.5
79	3879.5
80	3692.5
81	3529.0
82	3456.5
83	3410.0
84	3335.0
85	3226.5
86	3143.0
87	3061.5
88	3015.0
89	3002.0
90	2922.0
91	2818.0
92	2702.0
93	2713.0
94	2641.0
95	2428.0
96	2318.0
97	2179.5
98	1988.0
99	1735.0
100	1612.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15453803752452241
2	0.14494668158696875
3	0.1467724947182168
4	0.14310685965931727
5	0.1415191960669277
6	0.1254417673945824
7	0.12688700822353705
8	0.12814546510044586
9	0.12753374765749576
10-11	0.12709363797048776
12-13	0.13065770925546236
14-15	0.13850497004078802
16-17	0.13184845694975456
18-19	0.13919957286245846
20-21	0.1402852545837249
22-23	0.14038331615854896
24-25	0.14116430655804058
26-27	0.14179470239619527
28-29	0.1423025212658199
30-31	0.1406296374953094
32-33	0.14094483541438674
34-35	0.1426644151729087
36-37	0.14374075769657282
38-39	0.1437337532983711
40-41	0.14051289752528073
42-43	0.13788624819963619
44-45	0.1376340898643743
46-47	0.13823880290912272
48-49	0.13991635627843435
50-51	0.1373060505485938
52-53	0.13770179904699092
54-55	0.14045919713906754
56-57	0.1369885178301159
58-59	0.13799014677296167
60-61	0.13938518941480402
62-63	0.13428365272455214
64-65	0.12649826412334167
66-67	0.12667337407838464
68-69	0.12455337622266441
70-71	0.12327040395204956
72-73	0.1266757088777852
74-75	0.12791315256008887
76-77	0.1253051816296489
78-79	0.12070562681052019
80-81	0.12143525162319924
82-83	0.12213919364247197
84-85	0.11743340545061721
86-87	0.12136520764118204
88-89	0.12061340223419757
90-91	0.12628462997818923
92-93	0.12294936903447079
94-95	0.12497013791566668
96-97	0.12832641205399029
98-99	0.1317305495800256
100	0.13198154051558722
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.2830232E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.61486582973113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1839709477788	51.81076524485787
2	13.539138760554636	17.496592689267
3	4.009275972257378	7.771764870653261
4	1.268544876290174	3.2786742792192984
5	0.42644180551226346	1.37772400236816
6	0.17331971637141136	0.6719418131391479
7	0.08517012738712665	0.3852279447674208
8	0.058246933018254794	0.3010894209574296
9	0.044781468950650806	0.2604193747114115
>10	0.19427844804392064	2.1640795663836907
>50	0.010897411715957523	0.48059380874196367
>100	0.005081382989530305	0.5857819469017826
>500	3.6467255893636276E-4	0.1586908895892198
>1k	2.902102603090741E-4	0.35196269853599316
>5k	5.7386569770672906E-5	0.2604656065194169
>10k+	1.3987974078711598E-4	12.644225843386883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2292049	5.351474631283809	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	506685	1.1830078342793007	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402929	0.9407583876734547	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	387510	0.9047581157160204	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	222419	0.5193037478760331	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159568	0.37255927075062306	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	141306	0.32992116409736	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	136514	0.3187328053698145	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118759	0.2772784420126419	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	111026	0.25922343824801136	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93639	0.21862828107024965	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66973	0.1563685202545716	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57238	0.133639248089994	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56529	0.1319838753149878	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54730	0.12778357119335707	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53543	0.125012164304877	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52839	0.12336846552687362	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51783	0.1209029173598686	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	46694	0.10902112321035291	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001545637203179287	0.0	0.0	4.669598801145882E-6	0.0
2	0.0035535646876720165	0.0	0.0	1.867839520458353E-5	2.334799400572941E-6
3	0.005790302513420894	0.0	0.0	3.735679040916706E-5	2.334799400572941E-6
4	0.009348536799894057	0.0	0.0	3.969158980974E-5	2.334799400572941E-6
5	0.015517076816207766	0.0	0.0	5.370038621317765E-5	2.334799400572941E-6
6	0.027616007309976747	0.0	0.0	5.603518561375059E-5	2.334799400572941E-6
7	0.04185361405467054	0.0	0.0	5.603518561375059E-5	2.334799400572941E-6
8	0.08765303909630935	0.0	0.0	5.603518561375059E-5	2.334799400572941E-6
9	0.2574513255029765	0.0	0.0	6.303958381546942E-5	2.334799400572941E-6
10-11	0.6296650459423148	0.0	0.0	7.12113817174747E-5	2.334799400572941E-6
12-13	1.08471394691488	0.0	0.0	7.821577991919353E-5	2.334799400572941E-6
14-15	1.5678761674697443	0.0	0.0	8.405277842062588E-5	2.334799400572941E-6
16-17	2.065964760592471	0.0	0.0	1.0506597302578234E-4	2.334799400572941E-6
18-19	2.5936270903225553	0.0	0.0	1.3775316463380353E-4	2.334799400572941E-6
20-21	3.2663890310003456	0.0	0.0	1.424227634349494E-4	2.334799400572941E-6
22-23	4.0189917719801285	0.0	1.1673997002864706E-6	1.424227634349494E-4	2.334799400572941E-6
24-25	4.79253766358305	0.0	2.334799400572941E-6	1.4475756283552235E-4	2.334799400572941E-6
26-27	5.541450254110227	0.0	2.334799400572941E-6	1.4592496253580884E-4	2.334799400572941E-6
28-29	6.300932481523798	0.0	2.334799400572941E-6	1.5059456133695472E-4	2.334799400572941E-6
30-31	7.114489830454339	0.0	2.334799400572941E-6	1.622685583398194E-4	2.334799400572941E-6
32-33	8.0132988772977	0.0	2.334799400572941E-6	1.704403562418247E-4	2.334799400572941E-6
34-35	8.841831629583513	0.0	2.334799400572941E-6	1.8094695354440291E-4	2.334799400572941E-6
36-37	9.512812118318667	0.0	4.669598801145882E-6	1.9262095054726764E-4	2.334799400572941E-6
38-39	10.040658897201398	0.0	4.669598801145882E-6	2.3114514065672117E-4	2.334799400572941E-6
40-41	10.526159419355935	0.0	4.669598801145882E-6	2.638323322647424E-4	2.334799400572941E-6
42-43	10.945859457403827	0.0	4.669598801145882E-6	3.035239220744823E-4	2.334799400572941E-6
44-45	11.305739600009638	0.0	4.669598801145882E-6	3.327089145816441E-4	2.334799400572941E-6
46-47	11.626651940619887	0.0	4.669598801145882E-6	3.6656350588995176E-4	2.334799400572941E-6
48-49	11.93005912272434	0.0	4.669598801145882E-6	4.039202962991188E-4	2.334799400572941E-6
50-51	12.233391591247976	0.0	4.669598801145882E-6	4.587880822125829E-4	2.334799400572941E-6
52-53	12.54547605532466	0.0	4.669598801145882E-6	5.358364624314901E-4	2.334799400572941E-6
54-55	12.86219392881178	0.0	4.669598801145882E-6	6.584134309615694E-4	2.334799400572941E-6
56-57	13.186072632060457	0.0	4.669598801145882E-6	7.086116180738876E-4	2.334799400572941E-6
58-59	13.519193638736303	0.0	4.669598801145882E-6	7.284574129787576E-4	2.334799400572941E-6
60-61	13.853960678989552	0.0	4.669598801145882E-6	7.763208006905029E-4	2.334799400572941E-6
62-63	14.181621757267155	0.0	4.669598801145882E-6	8.346907857048264E-4	2.334799400572941E-6
64-65	14.498127164008825	0.0	4.669598801145882E-6	9.012325686211552E-4	2.334799400572941E-6
66-67	14.80097632905654	0.0	4.669598801145882E-6	0.0010133029398486566	2.334799400572941E-6
68-69	15.087448043708939	0.0	4.669598801145882E-6	0.0010728403245632663	2.334799400572941E-6
70-71	15.363634967001815	0.0	4.669598801145882E-6	0.0011008579173701417	2.334799400572941E-6
72-73	15.636397906973746	0.0	4.669598801145882E-6	0.0011312103095775898	2.334799400572941E-6
74-75	15.905315432332937	0.0	4.669598801145882E-6	0.001181408496689908	2.334799400572941E-6
76-77	16.172451505749493	0.0	4.669598801145882E-6	0.0012899766688165499	2.334799400572941E-6
78-79	16.43679749388236	0.0	4.669598801145882E-6	0.0014475756283552233	2.334799400572941E-6
80-81	16.698445621307865	0.0	4.669598801145882E-6	0.0016717163708102258	2.334799400572941E-6
82-83	16.960651765790107	0.0	4.669598801145882E-6	0.0017510995504297058	2.334799400572941E-6
84-85	17.225073634903495	2.334799400572941E-6	4.669598801145882E-6	0.0018001303378417377	2.334799400572941E-6
86-87	17.491176559585295	2.334799400572941E-6	4.669598801145882E-6	0.0018328175294497588	2.334799400572941E-6
88	17.690742370949568	2.334799400572941E-6	4.669598801145882E-6	0.001858500322856061	2.334799400572941E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGCTG	5930	0.0	36.537357	8
GCCTGCT	1985	0.0	35.98796	7
TTGCTGA	10595	0.0	35.35541	9
CCGTTTT	4260	0.0	35.31294	1
CTGCTGA	8710	0.0	34.58903	9
GCTTGCT	2010	0.0	33.90362	7
TACCCGA	13835	0.0	33.085964	6
CTTGCTG	6235	0.0	32.488655	8
CCCGACG	15335	0.0	32.487255	8
CTACCCG	13365	0.0	31.299788	5
ACCCGAC	15655	0.0	30.89132	7
CCTGTTT	2820	0.0	30.17331	1
CCTTTTG	3015	0.0	28.533646	1
TTGCGCC	1060	0.0	28.379759	3
CCTTGTT	1985	0.0	28.182526	1
TTGCCAA	2470	0.0	27.782816	5
GACACGC	1520	0.0	26.904156	2
TTTTGCC	3110	0.0	26.751457	3
GTTTGCC	1195	0.0	26.747032	3
CATGCCA	2120	0.0	26.605247	4
>>END_MODULE