##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248446_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42830232 Sequences flagged as poor quality 0 Sequence length 100 %GC 28 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65457709871849 34.0 31.0 34.0 31.0 34.0 2 32.904123003583074 34.0 31.0 34.0 31.0 34.0 3 33.00560151063389 34.0 33.0 34.0 31.0 34.0 4 36.375070254113965 37.0 37.0 37.0 35.0 37.0 5 36.28271516250484 37.0 37.0 37.0 35.0 37.0 6 36.19183001390233 37.0 37.0 37.0 35.0 37.0 7 36.23012254521526 37.0 37.0 37.0 35.0 37.0 8 35.920717590322646 37.0 35.0 37.0 35.0 37.0 9 37.7073709990644 39.0 38.0 39.0 35.0 39.0 10-11 37.94748326369094 39.0 38.5 39.0 35.0 39.0 12-13 38.05584846003169 39.0 39.0 39.0 35.0 39.0 14-15 39.619964281304846 41.0 40.0 41.0 37.0 41.0 16-17 39.60978696076174 41.0 40.0 41.0 37.0 41.0 18-19 39.505596689273126 41.0 39.0 41.0 37.0 41.0 20-21 39.25014648531439 41.0 39.0 41.0 35.5 41.0 22-23 38.947532457914306 40.5 38.5 41.0 35.0 41.0 24-25 38.680210686227426 40.0 37.0 41.0 35.0 41.0 26-27 38.53620113474987 40.0 37.0 41.0 35.0 41.0 28-29 38.4246139852803 40.0 37.0 41.0 35.0 41.0 30-31 38.35747993380003 40.0 37.0 41.0 35.0 41.0 32-33 38.28904318099421 40.0 36.0 41.0 35.0 41.0 34-35 38.21911620744898 40.0 36.0 41.0 35.0 41.0 36-37 38.14606237715453 40.0 36.0 41.0 35.0 41.0 38-39 38.0485237623742 40.0 35.0 41.0 34.0 41.0 40-41 37.93596683062562 40.0 35.0 41.0 34.0 41.0 42-43 37.7991676300049 40.0 35.0 41.0 33.5 41.0 44-45 37.675693888373054 40.0 35.0 41.0 33.0 41.0 46-47 37.54139434733858 40.0 35.0 41.0 33.0 41.0 48-49 37.40306758086204 39.0 35.0 41.0 33.0 41.0 50-51 37.168159712513344 39.0 35.0 41.0 33.0 41.0 52-53 36.87324184935538 38.5 35.0 41.0 33.0 41.0 54-55 36.66218775093257 38.0 35.0 41.0 32.5 41.0 56-57 36.42635806875853 37.0 35.0 40.5 32.0 41.0 58-59 36.17880301232083 37.0 35.0 40.0 32.0 41.0 60-61 35.901790363871946 36.0 35.0 40.0 31.0 41.0 62-63 35.622635093361154 35.5 35.0 40.0 31.0 41.0 64-65 35.38269868582547 35.0 35.0 39.0 31.0 41.0 66-67 35.147726248599355 35.0 35.0 39.0 31.0 41.0 68-69 34.938665193314854 35.0 35.0 39.0 30.5 41.0 70-71 34.79373017405089 35.0 35.0 37.5 31.0 40.0 72-73 34.63385415470082 35.0 35.0 37.0 31.0 40.0 74-75 34.41886060761941 35.0 34.0 37.0 30.5 39.0 76-77 34.20546107478475 35.0 34.0 36.0 30.0 39.0 78-79 33.99505552526543 35.0 34.0 36.0 30.0 39.0 80-81 33.80497497655394 35.0 34.0 36.0 30.0 37.5 82-83 33.63431830114766 35.0 34.0 35.0 29.5 37.0 84-85 33.46783377218223 35.0 34.0 35.0 29.0 37.0 86-87 33.32153183293521 35.0 34.0 35.0 29.0 36.0 88-89 33.18308614345119 35.0 34.0 35.0 29.0 36.0 90-91 33.065048912179606 35.0 34.0 35.0 29.0 36.0 92-93 32.92778879880922 35.0 34.0 35.0 29.0 36.0 94-95 32.78080973271403 35.0 34.0 35.0 29.0 36.0 96-97 32.65970013891123 35.0 34.0 35.0 27.0 35.0 98-99 32.52253073249755 35.0 33.0 35.0 27.0 35.0 100 31.871696725808068 35.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22038675472381897 1101 2 0.21595861294222374 1101 3 0.21407297524221036 1101 4 0.11130575053628178 1101 5 0.12774452342218723 1101 6 0.0018064414665417416 1101 7 0.06785088922375593 1101 8 -0.03967127112440494 1101 9 -0.06933620682646335 1101 10-11 0.058955516674146224 1101 12-13 0.14255383349478734 1101 14-15 0.21572672358698242 1101 16-17 0.24126122104784287 1101 18-19 0.2670356725746359 1101 20-21 0.27798994053689086 1101 22-23 0.2601928004859815 1101 24-25 0.2586723618409721 1101 26-27 0.2789624326310971 1101 28-29 0.3087905739296346 1101 30-31 0.31171266757873894 1101 32-33 0.3099117166726941 1101 34-35 0.3290676302667066 1101 36-37 0.3189397856218221 1101 38-39 0.29985078610378935 1101 40-41 0.30511436248035295 1101 42-43 0.3250010146802893 1101 44-45 0.31197776689336365 1101 46-47 0.31583889137288423 1101 48-49 0.2880937398837915 1101 50-51 0.30296050510902006 1101 52-53 0.24231601274985337 1101 54-55 0.294715313298596 1101 56-57 0.2819745513990455 1101 58-59 0.2707004521419236 1101 60-61 0.2890827594979726 1101 62-63 0.26646425267509244 1101 64-65 0.2624261917245789 1101 66-67 0.256084559232832 1101 68-69 0.25853481588464433 1101 70-71 0.2605616794454093 1101 72-73 0.2781234577499845 1101 74-75 0.2677903474985186 1101 76-77 0.2759901055270788 1101 78-79 0.31704014175456763 1101 80-81 0.31114322347885803 1101 82-83 0.2908635768250605 1101 84-85 0.261546372263318 1101 86-87 0.2826123310314159 1101 88-89 0.30502891973779356 1101 90-91 0.28949554406206346 1101 92-93 0.3089169667860858 1101 94-95 0.3135661817298967 1101 96-97 0.2986139192305757 1101 98-99 0.31786318681748327 1101 100 0.2634470997194285 1102 1 0.1644690164070326 1102 2 0.17123223245545205 1102 3 0.16468871750551273 1102 4 0.0999769260896528 1102 5 0.1124216687180919 1102 6 -0.02879813341350257 1102 7 0.01039586876680687 1102 8 -0.06727718139080707 1102 9 -0.05244101226136877 1102 10-11 0.0348520029824968 1102 12-13 0.1150820025687409 1102 14-15 0.17973750972785751 1102 16-17 0.2312135954412895 1102 18-19 0.25596911191129834 1102 20-21 0.2335295513956055 1102 22-23 0.20745453437982064 1102 24-25 0.19907239982121183 1102 26-27 0.221666246839888 1102 28-29 0.23333610685014605 1102 30-31 0.22013914537991752 1102 32-33 0.22222779860777564 1102 34-35 0.25510931970391226 1102 36-37 0.24091013883165857 1102 38-39 0.24162567852051353 1102 40-41 0.231525837790457 1102 42-43 0.23483838892383346 1102 44-45 0.26438644590022164 1102 46-47 0.2739420250815314 1102 48-49 0.27129780768256495 1102 50-51 0.23893866686231746 1102 52-53 0.2456734386078665 1102 54-55 0.2507407392684087 1102 56-57 0.23418567851432215 1102 58-59 0.2393424126268684 1102 60-61 0.2536726725621534 1102 62-63 0.22772479025584857 1102 64-65 0.2166606072236945 1102 66-67 0.23582840365219937 1102 68-69 0.2307695576613682 1102 70-71 0.22403122393740205 1102 72-73 0.19070079946580165 1102 74-75 0.17633983039974765 1102 76-77 0.20771698466569433 1102 78-79 0.19192180493243427 1102 80-81 0.19810810087088981 1102 82-83 0.18599526312200965 1102 84-85 0.17418255673337768 1102 86-87 0.20102322521199767 1102 88-89 0.19680775336540535 1102 90-91 0.22418878450147872 1102 92-93 0.24529242382763528 1102 94-95 0.23094932490355546 1102 96-97 0.24498630754168005 1102 98-99 0.26208521284249287 1102 100 0.2441531525746683 1103 1 0.16082811854897017 1103 2 0.16392754046911762 1103 3 0.15207851940292016 1103 4 0.09554764721251985 1103 5 0.11174973247418052 1103 6 0.03659478162231977 1103 7 0.04921203095499038 1103 8 -0.02765985802334825 1103 9 -0.11514753941163036 1103 10-11 -3.612461441235837E-4 1103 12-13 0.08366179846592559 1103 14-15 0.19306271407960196 1103 16-17 0.20755833290993309 1103 18-19 0.21204529886788492 1103 20-21 0.21536723603720276 1103 22-23 0.17377632303725932 1103 24-25 0.17111027253424993 1103 26-27 0.17195272461947297 1103 28-29 0.1966987146100152 1103 30-31 0.1998606983748985 1103 32-33 0.2075666002311891 1103 34-35 0.22696272096460746 1103 36-37 0.21290618136121253 1103 38-39 0.21960504443235607 1103 40-41 0.237000758164001 1103 42-43 0.23350863608114025 1103 44-45 0.24045428506981636 1103 46-47 0.2412059377280471 1103 48-49 0.2170584228293322 1103 50-51 0.24066535512918108 1103 52-53 0.17123377251304817 1103 54-55 0.18500064639131608 1103 56-57 0.19911604848950049 1103 58-59 0.20037135865669597 1103 60-61 0.2046450522601404 1103 62-63 0.16966766062299143 1103 64-65 0.1410986592526129 1103 66-67 0.13842892521306993 1103 68-69 0.1413449964686322 1103 70-71 0.16958329658947235 1103 72-73 0.15112356814424288 1103 74-75 0.1576831141109949 1103 76-77 0.13353961353019628 1103 78-79 0.17650150770960238 1103 80-81 0.17106848899378235 1103 82-83 0.16247934551512344 1103 84-85 0.154450105743976 1103 86-87 0.17754737309405755 1103 88-89 0.18066566951563345 1103 90-91 0.1776202072682338 1103 92-93 0.1546764768837292 1103 94-95 0.14978729245009959 1103 96-97 0.13944244299300834 1103 98-99 0.1584242523111712 1103 100 0.1296454701509191 1104 1 -0.2373981550736204 1104 2 -0.20299727023746783 1104 3 -0.1757484284265587 1104 4 -0.21310020332513346 1104 5 -0.09704441724187518 1104 6 -0.045348856152656936 1104 7 0.011287469202216016 1104 8 -0.13737356121048094 1104 9 -0.024937307834257183 1104 10-11 0.06933176564553634 1104 12-13 0.10306724607779216 1104 14-15 0.13642254622172345 1104 16-17 0.17287467801256184 1104 18-19 0.16986097179387372 1104 20-21 0.1588614324719302 1104 22-23 0.1483918335354133 1104 24-25 0.1410119640244858 1104 26-27 0.1572990802618648 1104 28-29 0.17699778107078146 1104 30-31 0.1774415863927743 1104 32-33 0.17881951762798565 1104 34-35 0.1853217401963292 1104 36-37 0.18401843687913555 1104 38-39 0.19847515330504706 1104 40-41 0.2133002949843572 1104 42-43 0.19295507095861097 1104 44-45 0.19650332564171435 1104 46-47 0.214899674236527 1104 48-49 0.22301019660205412 1104 50-51 0.2178589191817224 1104 52-53 0.1647264521825349 1104 54-55 0.22831146509687983 1104 56-57 0.19902724888680723 1104 58-59 0.2078049510111839 1104 60-61 0.21241537140236488 1104 62-63 0.16607748423787427 1104 64-65 0.15356098290894948 1104 66-67 0.16498493658296098 1104 68-69 0.15920323867614172 1104 70-71 0.18414620564210082 1104 72-73 0.1782145039504428 1104 74-75 0.17629509798790366 1104 76-77 0.17426299107493293 1104 78-79 0.1795918052566634 1104 80-81 0.16769278994480175 1104 82-83 0.1722142443463781 1104 84-85 0.17805484172233577 1104 86-87 0.186390435782954 1104 88-89 0.1753843599310585 1104 90-91 0.18448238943919648 1104 92-93 0.1917733022311694 1104 94-95 0.17458965225673495 1104 96-97 0.19505289587739583 1104 98-99 0.21708274371467695 1104 100 0.2132432970335998 1105 1 0.1355901191045632 1105 2 0.1346456896139756 1105 3 0.11672716840502773 1105 4 0.06680190780919304 1105 5 0.0929743954173432 1105 6 0.02795126849093066 1105 7 0.048418905440733795 1105 8 -0.0900143593226872 1105 9 -0.057735056067521384 1105 10-11 0.054110730641760085 1105 12-13 0.1045330986834756 1105 14-15 0.15045251992508213 1105 16-17 0.15890474172326208 1105 18-19 0.16837629780350483 1105 20-21 0.17138748980192275 1105 22-23 0.13915552319509317 1105 24-25 0.12460223828112049 1105 26-27 0.14093875281484713 1105 28-29 0.16341321018089872 1105 30-31 0.1673355112296946 1105 32-33 0.17038095754901406 1105 34-35 0.1748224113421344 1105 36-37 0.15664966257776314 1105 38-39 0.14856792493223736 1105 40-41 0.1418634906403824 1105 42-43 0.14826684153347713 1105 44-45 0.17653961228737103 1105 46-47 0.18272565844862498 1105 48-49 0.14255993446582949 1105 50-51 0.14641539333576503 1105 52-53 -0.06230353965993629 1105 54-55 -0.018857987197968384 1105 56-57 0.024993567622033197 1105 58-59 0.043447814616676794 1105 60-61 0.08174602699778433 1105 62-63 0.022920069896372297 1105 64-65 0.026065836866720815 1105 66-67 0.04289220298979757 1105 68-69 0.058572220735754854 1105 70-71 0.03428769607275939 1105 72-73 0.01843442742219281 1105 74-75 0.014712857931172607 1105 76-77 6.121242974543861E-4 1105 78-79 0.022835037919719525 1105 80-81 0.030600610900997083 1105 82-83 0.025064322139037643 1105 84-85 0.0034879959771885183 1105 86-87 0.031856886829025655 1105 88-89 0.02367661916852626 1105 90-91 0.022239710761681408 1105 92-93 0.020010989925751232 1105 94-95 0.010146879017469246 1105 96-97 0.023433289136143287 1105 98-99 -0.02279823709906026 1105 100 -0.03850417664370198 1106 1 0.12170268161476372 1106 2 0.10912377119492334 1106 3 0.10417204767455246 1106 4 0.07714424455228652 1106 5 0.07209235394874014 1106 6 0.048122819443499054 1106 7 0.05952733078137129 1106 8 -0.04633722869083101 1106 9 -0.02810663654750556 1106 10-11 0.06317331391784364 1106 12-13 0.12364630859856618 1106 14-15 0.14054515168245985 1106 16-17 0.13250721507436936 1106 18-19 0.12000287685360433 1106 20-21 0.130878096649667 1106 22-23 0.1316628561779396 1106 24-25 0.12794557723388778 1106 26-27 0.1525127639335011 1106 28-29 0.13292614633991917 1106 30-31 0.13895979878606113 1106 32-33 0.13810729670889543 1106 34-35 0.1284303204617956 1106 36-37 0.15879954948334074 1106 38-39 0.16006597427674052 1106 40-41 0.15877507379143907 1106 42-43 0.16071451445643703 1106 44-45 0.1703223727813281 1106 46-47 0.18735477028709369 1106 48-49 0.17739158408154054 1106 50-51 0.17945279086908528 1106 52-53 0.07467528080268693 1106 54-55 0.11583909280181359 1106 56-57 0.13776668516103996 1106 58-59 0.12987260135179923 1106 60-61 0.13731085771285478 1106 62-63 0.10037940481475971 1106 64-65 0.11866533672073132 1106 66-67 0.1288789854167689 1106 68-69 0.1101065638114278 1106 70-71 0.10100550731001334 1106 72-73 0.11227837951720687 1106 74-75 0.10278235171225703 1106 76-77 0.1045931660062891 1106 78-79 0.12119149990031985 1106 80-81 0.1002235995563936 1106 82-83 0.0934106369293417 1106 84-85 0.0979141823981351 1106 86-87 0.09622976160038377 1106 88-89 0.08037632825870844 1106 90-91 0.07834242724347007 1106 92-93 0.08537141486540634 1106 94-95 0.08704219814787706 1106 96-97 0.08807302650002669 1106 98-99 0.11371257871673635 1106 100 0.10946997329337194 1107 1 0.04356293264435607 1107 2 0.04549087131574936 1107 3 0.06807999912965101 1107 4 0.028991998814490216 1107 5 0.01568366145057354 1107 6 0.005772837281682541 1107 7 0.004969805768212154 1107 8 -0.08638871309609897 1107 9 -0.095941362461204 1107 10-11 -0.009646269230572102 1107 12-13 0.023963364257539865 1107 14-15 0.050571154773713545 1107 16-17 0.044370966422135893 1107 18-19 0.06318547860846024 1107 20-21 0.03169980576623033 1107 22-23 0.04297568575687194 1107 24-25 0.0628713536275356 1107 26-27 0.06772862815221004 1107 28-29 0.08017353043785391 1107 30-31 0.06877301830890303 1107 32-33 0.06225286942498087 1107 34-35 0.08091922384645045 1107 36-37 0.078395331685023 1107 38-39 0.07116711641891271 1107 40-41 0.05923804486626949 1107 42-43 0.05125448495903129 1107 44-45 0.038004989658873 1107 46-47 0.0872626544930526 1107 48-49 0.09567346590919357 1107 50-51 0.08381490457680485 1107 52-53 0.027202833371262614 1107 54-55 0.04496499414179311 1107 56-57 0.07587609680398089 1107 58-59 0.06828775992252645 1107 60-61 0.10458928577478588 1107 62-63 0.03585674951854401 1107 64-65 0.04351123352791575 1107 66-67 0.03274119952438781 1107 68-69 0.05304298410827357 1107 70-71 0.045599932596935844 1107 72-73 0.027611735625811207 1107 74-75 0.0442918299613666 1107 76-77 0.04733833263858145 1107 78-79 0.04281835320059457 1107 80-81 0.030773370339396422 1107 82-83 0.050480340764934795 1107 84-85 0.06048331967878795 1107 86-87 0.04101334291178915 1107 88-89 0.07107199545144738 1107 90-91 0.033661846610826274 1107 92-93 0.054893678622597974 1107 94-95 0.07086268549080188 1107 96-97 0.06325090931015609 1107 98-99 0.07515948457109545 1107 100 0.05532321572103882 1108 1 0.048279604401905374 1108 2 0.03158375135903668 1108 3 0.049313183217904566 1108 4 0.024863919461651562 1108 5 0.013077548522559823 1108 6 0.007533586989502794 1108 7 0.037734524022745575 1108 8 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2214 42-43 -0.2536433322182532 2214 44-45 -0.2385773850316042 2214 46-47 -0.24509829055596555 2214 48-49 -0.21247576667142454 2214 50-51 -0.238862636481592 2214 52-53 -0.19327380590792842 2214 54-55 -0.20455448577183688 2214 56-57 -0.20289862721598695 2214 58-59 -0.2158755552750833 2214 60-61 -0.17415480325745847 2214 62-63 -0.1502737266051355 2214 64-65 -0.1430541346605807 2214 66-67 -0.174826554041708 2214 68-69 -0.1610425877170485 2214 70-71 -0.14878265038128546 2214 72-73 -0.1308232246881431 2214 74-75 -0.1316307684390381 2214 76-77 -0.11228494236365805 2214 78-79 -0.136134050259912 2214 80-81 -0.14925658967975863 2214 82-83 -0.156953201557684 2214 84-85 -0.13220871008363844 2214 86-87 -0.14757658147455288 2214 88-89 -0.15940589188313936 2214 90-91 -0.12525041031812378 2214 92-93 -0.11425005046872627 2214 94-95 -0.11306590573626352 2214 96-97 -0.12592432334707127 2214 98-99 -0.10242785435779211 2214 100 -0.1263109335368604 2215 1 -0.2557477164714541 2215 2 -0.24276069430113267 2215 3 -0.2502837981353707 2215 4 -0.16699769468371528 2215 5 -0.20664866296161932 2215 6 -0.12345801162609149 2215 7 -0.12740683517284168 2215 8 0.13931895990936027 2215 9 0.09865097211676499 2215 10-11 -0.06569548977009987 2215 12-13 -0.1793219295038071 2215 14-15 -0.28591571222177947 2215 16-17 -0.33427224119234467 2215 18-19 -0.36913885482599085 2215 20-21 -0.3921097833097633 2215 22-23 -0.3640745163134369 2215 24-25 -0.3832139652725459 2215 26-27 -0.43268046921654957 2215 28-29 -0.4292184263219454 2215 30-31 -0.4260167260680987 2215 32-33 -0.41683732187224365 2215 34-35 -0.43797030967827766 2215 36-37 -0.4386923900887041 2215 38-39 -0.450134419663641 2215 40-41 -0.45362846025386716 2215 42-43 -0.49342358637647266 2215 44-45 -0.5132697450710566 2215 46-47 -0.5045730008868148 2215 48-49 -0.49001372479154526 2215 50-51 -0.4679795445290651 2215 52-53 -0.40869965149538245 2215 54-55 -0.4151058440210136 2215 56-57 -0.40305069154689477 2215 58-59 -0.42761188784992044 2215 60-61 -0.37229778715686024 2215 62-63 -0.35168400101040476 2215 64-65 -0.3533416868765329 2215 66-67 -0.3605726034967276 2215 68-69 -0.326997033660831 2215 70-71 -0.31628427662098346 2215 72-73 -0.3454160757806335 2215 74-75 -0.3578034628326492 2215 76-77 -0.37366301735594476 2215 78-79 -0.36262608123426787 2215 80-81 -0.3764474656825385 2215 82-83 -0.3761323502036049 2215 84-85 -0.3751010408948332 2215 86-87 -0.3996462677602963 2215 88-89 -0.3923369371494374 2215 90-91 -0.346193603992063 2215 92-93 -0.3528488982939777 2215 94-95 -0.3494817279736324 2215 96-97 -0.37085054401918427 2215 98-99 -0.358585799712138 2215 100 -0.30613831437912253 2216 1 -0.26673990373257084 2216 2 -0.2598240864903971 2216 3 -0.293371676570537 2216 4 -0.174782956992658 2216 5 -0.21282933056742337 2216 6 -0.14230503294256636 2216 7 -0.18916591652877202 2216 8 0.0709005935989282 2216 9 0.03760800838458067 2216 10-11 -0.1673909629093302 2216 12-13 -0.2791327828087873 2216 14-15 -0.4146591460099387 2216 16-17 -0.44358740187179535 2216 18-19 -0.46785096512394375 2216 20-21 -0.42746419082886433 2216 22-23 -0.34185541509823736 2216 24-25 -0.29685895913398497 2216 26-27 -0.3154075156456315 2216 28-29 -0.322382897161809 2216 30-31 -0.3367500934364216 2216 32-33 -0.3709398706448326 2216 34-35 -0.370501214542152 2216 36-37 -0.36192387423648853 2216 38-39 -0.35627045374253896 2216 40-41 -0.36712350139227823 2216 42-43 -0.36910813185892977 2216 44-45 -0.40794554732266164 2216 46-47 -0.3865959226915905 2216 48-49 -0.36070067498750547 2216 50-51 -0.3671708273344123 2216 52-53 -0.3379193027055294 2216 54-55 -0.33199206254421654 2216 56-57 -0.3294784127024002 2216 58-59 -0.35020660688830674 2216 60-61 -0.3326968928262133 2216 62-63 -0.31731769209272187 2216 64-65 -0.3295820203034694 2216 66-67 -0.3353530573727639 2216 68-69 -0.3427831633305516 2216 70-71 -0.31127271306152693 2216 72-73 -0.31203651754260875 2216 74-75 -0.27131364172434047 2216 76-77 -0.29399580835728756 2216 78-79 -0.318105037750982 2216 80-81 -0.3233589435790165 2216 82-83 -0.34500496696202276 2216 84-85 -0.3451135874607587 2216 86-87 -0.3687279625945479 2216 88-89 -0.39408682757738234 2216 90-91 -0.3879760113619142 2216 92-93 -0.3809632201880504 2216 94-95 -0.38868416547449414 2216 96-97 -0.3920302124856434 2216 98-99 -0.38878034812363893 2216 100 -0.3284933381257211 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 30.0 7 226.0 8 935.0 9 1938.0 10 3250.0 11 5476.0 12 8500.0 13 11808.0 14 15884.0 15 20945.0 16 28638.0 17 39372.0 18 53428.0 19 70124.0 20 90502.0 21 114914.0 22 141263.0 23 171019.0 24 205656.0 25 244261.0 26 288966.0 27 344952.0 28 413775.0 29 503002.0 30 599258.0 31 737401.0 32 940165.0 33 1288274.0 34 1843987.0 35 6927287.0 36 4892868.0 37 1.0004263E7 38 9091908.0 39 3681435.0 40 44521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.87458958296387 23.037971609642685 33.016537633562486 25.070901173830958 2 15.04862452362284 25.184647296801117 37.50358218254041 22.263145997035632 3 12.714782513435734 22.5586015433134 40.363597767238275 24.36301817601259 4 12.944883705465632 20.50025525061632 43.475705987901144 23.07915505601691 5 11.468930727859316 21.034171125144958 45.652294222345475 21.84460392465025 6 10.253551131289084 17.064254612322625 43.14509577970968 29.537098476678615 7 13.472658751883483 18.24808000106093 40.892718022167145 27.386543224888438 8 7.337447997012951 18.80851124037806 43.89850141367434 29.95553934893465 9 9.57500300255203 16.45823912417752 44.5275290593803 29.43922881389015 10-11 13.757567552282326 29.30507695058764 42.539583050887195 14.397772446242843 12-13 12.197960017859335 32.27518422401109 41.1891564826412 14.337699275488372 14-15 12.905238501642327 30.888429016403013 41.14875112383923 15.057581358115424 16-17 15.061382872893445 31.074155184732906 41.20102785076797 12.663434091605685 18-19 15.154212377253822 28.885740643282258 41.06695173652397 14.89309524293995 20-21 12.683078546816432 28.37575372854516 43.644170479092075 15.296997245546331 22-23 12.531090955101371 28.607154046265247 44.54633819378048 14.315416804852903 24-25 12.475392893027163 28.901784646528778 43.93543060575195 14.687391854692116 26-27 14.141581130971174 28.768879389179514 42.251946346864514 14.8375931329848 28-29 13.30038505885083 30.181910356906094 43.33893530582163 13.178769278421443 30-31 13.337920224287576 29.26588155151073 43.39126034300676 14.004937881194932 32-33 13.657039628122963 29.562272862221704 43.27814485304033 13.502542656615002 34-35 13.544167953230236 29.66085014551635 43.268431597697735 13.526550303555688 36-37 13.659361676963751 29.160050805239514 43.22206988682154 13.958517630975203 38-39 13.879475687631176 29.314839827485894 42.983947837127836 13.821736647755095 40-41 13.979638136063969 29.808237360100076 42.826377859956786 13.38574664387917 42-43 14.00504970437936 29.982085083362847 42.80716330498342 13.205701907274372 44-45 14.170250255113393 29.521054577338756 42.75661479504984 13.552080372498008 46-47 14.348632030158917 29.38817984064696 42.85171994591478 13.411468183279343 48-49 14.090150602002804 29.32243206814875 43.00923875348384 13.578178576364605 50-51 13.936109545711275 29.22724226856553 43.25863392455116 13.578014261172033 52-53 13.811072300670634 29.307874907218117 43.39643422105253 13.484618571058723 54-55 13.833842782086576 29.314432604807372 43.397344064390076 13.454380548715974 56-57 13.822948746367455 29.308646983316017 43.21994115643798 13.648463113878547 58-59 13.743808128516143 29.432128641195522 43.414422401882454 13.409640828405884 60-61 13.612728257967191 29.339859686009202 43.520153721880064 13.527258334143541 62-63 13.476952990622335 29.24276560563181 43.6311883983302 13.649093005415654 64-65 13.590911357639929 29.182723609964096 43.54464730162263 13.681717730773348 66-67 13.51592535190772 29.01968284026587 43.862937929123305 13.6014538787031 68-69 13.552565829857846 29.112369199533163 43.88471927911555 13.450345691493443 70-71 13.475308323914062 29.170679833110192 43.9544815356747 13.399530307301044 72-73 13.376114619202085 28.959031093205617 44.27864254982507 13.386211737767228 74-75 13.366070027165675 28.70951896402155 44.44610992889348 13.47830107991929 76-77 13.529967323641637 28.660715314625694 44.41373725225463 13.395580109478042 78-79 13.634688964855615 28.814840901860183 44.332273219654276 13.218196913629926 80-81 13.54114883519787 28.68370904383503 44.47428246035556 13.300859660611536 82-83 13.491076304890123 28.516698380797074 44.63940632670369 13.352818987609114 84-85 13.452265290486467 28.497356199517938 44.5744258058493 13.475952704146293 86-87 13.504766219333131 28.68587062170408 44.37018482705439 13.439178331908405 88-89 13.491128890375844 29.080841948510372 44.17296396146704 13.25506519964674 90-91 13.474584076585169 29.28063177000613 44.15577007295993 13.089014080448763 92-93 13.424495129832831 29.363116107482725 44.040914802756504 13.171473959927948 94-95 13.435529409869204 29.57600136086048 43.89085842906311 13.09761080020721 96-97 13.323490813265268 29.955650286373668 43.92993590492714 12.790922995433924 98-99 13.224522069347314 30.43642578456323 43.76288902079529 12.576163125294165 100 13.233723616473075 30.81483443928812 43.49051368942444 12.460928254814373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 458489.0 1 1188332.0 2 2125026.0 3 1692688.5 4 647833.5 5 166748.0 6 75318.5 7 56598.0 8 57772.5 9 72398.5 10 102481.5 11 152746.0 12 225712.0 13 320384.0 14 435164.5 15 556839.5 16 677243.5 17 792804.5 18 891111.0 19 969040.0 20 1038335.0 21 1113986.5 22 1201915.5 23 1296810.5 24 1370587.0 25 1404308.0 26 1411285.5 27 1405693.0 28 1387706.5 29 1346805.0 30 1272879.0 31 1162186.0 32 1030394.5 33 898952.0 34 776415.5 35 667290.5 36 574064.5 37 496067.5 38 428940.0 39 368380.0 40 315764.0 41 271709.0 42 238757.5 43 222979.5 44 226007.0 45 247375.0 46 284272.0 47 333528.0 48 399260.5 49 496416.5 50 633021.5 51 798339.0 52 960055.0 53 1070896.0 54 1087326.0 55 988144.5 56 797765.5 57 572034.0 58 364342.0 59 208757.0 60 109774.5 61 54010.5 62 25878.0 63 12711.5 64 6761.5 65 4061.0 66 2813.5 67 2181.0 68 1679.0 69 1246.0 70 972.0 71 757.0 72 545.5 73 416.0 74 318.0 75 204.0 76 136.0 77 102.0 78 84.5 79 61.5 80 41.5 81 43.5 82 39.5 83 29.5 84 25.5 85 21.0 86 16.5 87 10.5 88 9.5 89 8.5 90 7.5 91 5.0 92 3.0 93 4.0 94 4.0 95 3.0 96 1.5 97 0.5 98 0.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007861269581729093 2 4.6229028131344234E-4 3 1.074007724263553E-4 4 0.0040532117593946254 5 0.013611880505340245 6 0.006756909465258092 7 0.0 8 0.0 9 0.0 10-11 0.011575935428040642 12-13 0.005159906675266199 14-15 0.0011323777092778764 16-17 1.9729054934841352E-4 18-19 6.654178291632883E-5 20-21 7.5414020638506E-4 22-23 1.1790736972893353E-4 24-25 2.941847244721906E-4 26-27 0.0025344247493219274 28-29 0.0015759895953867354 30-31 0.002173698241933408 32-33 0.0021328392524233818 34-35 0.0023884997867861186 36-37 9.28082761727744E-4 38-39 0.0010705055251626935 40-41 4.085898951002647E-5 42-43 4.669598801145882E-6 44-45 6.023782453478188E-4 46-47 1.1207037122750118E-4 48-49 3.677309055902382E-4 50-51 0.03197507779084643 52-53 0.03603879614754363 54-55 6.490742333592776E-4 56-57 1.354183652332306E-4 58-59 4.4010968700799937E-4 60-61 0.004006515771383167 62-63 0.012841396703151178 64-65 0.016919123856251817 66-67 0.017925422397898755 68-69 0.01855231603695259 70-71 0.016638947928183063 72-73 0.014320492123414134 74-75 0.015228729090237009 76-77 0.013648069896049126 78-79 0.014862165584347055 80-81 0.014974235955574558 82-83 0.013391241961986105 84-85 0.013545338722423916 86-87 0.0122611990521088 88-89 0.010510099501679094 90-91 0.00818930889750959 92-93 0.008816202536563424 94-95 0.010242764970313492 96-97 0.005713254133201987 98-99 0.008332899060644827 100 0.009077700069427595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.2830232E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.14292090167025 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36248013081 47.30168003395153 2 14.629616606387891 17.889949819722727 3 4.830521925134676 8.860566599468802 4 1.7392933358204539 4.253818994274888 5 0.6372007901216518 1.9480158754444972 6 0.2638164035060912 0.9678303295281634 7 0.124833986766958 0.5342900205112585 8 0.07928318438735951 0.3878084377463089 9 0.04987836682067968 0.27447381334991805 >10 0.2484076306278108 2.7332378039620653 >50 0.020246380640047246 0.8586788800828868 >100 0.011846469877800287 1.3059735187506396 >500 8.108794070450857E-4 0.3408118936539462 >1k 9.664735657994568E-4 1.2777484430301167 >5k 1.7087919025006643E-4 0.7499612024199126 >10k+ 6.265569356073246E-4 10.315154334102292 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78534 0.18336113612459534 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52813 0.12330776074245874 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49765 0.1161912921695124 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49579 0.11575701948100584 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49316 0.11514296723865516 No Hit CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49114 0.11467133775973942 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49057 0.11453825419390677 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47386 0.11063680439554939 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45821 0.10698284333365274 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44681 0.10432117201699957 No Hit CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44639 0.10422311044217551 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44030 0.10280121760722659 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43621 0.10184628465239227 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43420 0.1013769899728771 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001183743296090481 0.0 0.0 4.669598801145882E-6 0.0 2 0.0028367812716961235 0.0 0.0 1.1673997002864705E-5 0.0 3 0.004585546022725256 0.0 0.0 3.502199100859412E-5 0.0 4 0.007700168423089559 0.0 0.0 3.502199100859412E-5 0.0 5 0.01370994208016431 0.0 0.0 3.502199100859412E-5 0.0 6 0.02626882805584616 0.0 0.0 3.735679040916706E-5 0.0 7 0.03998343973481162 0.0 0.0 3.735679040916706E-5 0.0 8 0.09755725815353977 0.0 0.0 3.735679040916706E-5 0.0 9 0.3339160058717403 0.0 0.0 4.436118861088588E-5 0.0 10-11 0.8012506212901205 0.0 1.1673997002864706E-6 4.669598801145882E-5 0.0 12-13 1.2602955314367663 0.0 2.334799400572941E-6 5.13655868126047E-5 0.0 14-15 1.7122870125942815 0.0 2.334799400572941E-6 6.654178291632883E-5 0.0 16-17 2.1303982196500826 0.0 2.334799400572941E-6 9.339197602291764E-5 0.0 18-19 2.561537607361081 0.0 2.334799400572941E-6 1.3892056433409E-4 0.0 20-21 3.1187701714994214 0.0 2.334799400572941E-6 1.4942716163666823E-4 0.0 22-23 3.775488538096175 0.0 3.5021991008594117E-6 1.5643155983838705E-4 0.0 24-25 4.515406313932645 0.0 4.669598801145882E-6 1.5876635923896E-4 0.0 26-27 5.26516877144163 0.0 8.171797902005294E-6 1.6110115863953294E-4 0.0 28-29 6.052353393742999 0.0 9.339197602291765E-6 1.622685583398194E-4 0.0 30-31 6.8956455804395365 0.0 9.339197602291765E-6 1.6577075744067882E-4 0.0 32-33 7.842823499064867 0.0 9.339197602291765E-6 1.8094695354440291E-4 0.0 34-35 8.735925128773527 0.0 9.339197602291765E-6 1.8444915264526234E-4 0.0 36-37 9.419225186545802 0.0 9.339197602291765E-6 1.9145355084698116E-4 0.0 38-39 9.932654579129995 0.0 9.339197602291765E-6 2.3231254035700765E-4 0.0 40-41 10.460695146362971 0.0 9.339197602291765E-6 2.7200413016674767E-4 0.0 42-43 10.907140311544424 0.0 1.0506597302578235E-5 3.2336971697935235E-4 0.0 44-45 11.267983558902973 0.0 1.1673997002864705E-5 3.502199100859412E-4 0.0 46-47 11.58236429819012 2.334799400572941E-6 1.1673997002864705E-5 3.642287064893788E-4 0.0 48-49 11.872571691883435 2.334799400572941E-6 1.1673997002864705E-5 4.027528965988323E-4 0.0 50-51 12.159272216877088 2.334799400572941E-6 1.1673997002864705E-5 4.914752738206041E-4 0.0 52-53 12.464477194519983 2.334799400572941E-6 1.1673997002864705E-5 6.105500432498241E-4 0.0 54-55 12.766414620401775 2.334799400572941E-6 1.1673997002864705E-5 7.12113817174747E-4 0.0 56-57 13.070494224733595 2.334799400572941E-6 1.1673997002864705E-5 7.436336090824818E-4 0.0 58-59 13.36571793493904 2.334799400572941E-6 1.1673997002864705E-5 7.693164024887841E-4 0.0 60-61 13.654417048219585 3.5021991008594117E-6 1.1673997002864705E-5 7.926643964945134E-4 0.0 62-63 13.945895740186511 4.669598801145882E-6 1.1673997002864705E-5 8.545365806096964E-4 0.0 64-65 14.23379868687146 4.669598801145882E-6 1.1673997002864705E-5 9.152413650245929E-4 0.0 66-67 14.510966225912576 4.669598801145882E-6 1.1673997002864705E-5 0.0010016289428457917 0.0 68-69 14.77631501038799 4.669598801145882E-6 1.1673997002864705E-5 0.0010798447227649854 0.0 70-71 15.03789893082998 4.669598801145882E-6 1.1673997002864705E-5 0.001148721305081887 0.0 72-73 15.302603310670836 4.669598801145882E-6 1.1673997002864705E-5 0.0011790736972893353 0.0 74-75 15.564871327337194 4.669598801145882E-6 1.1673997002864705E-5 0.0012012542915947782 0.0 76-77 15.826543970156408 4.669598801145882E-6 1.1673997002864705E-5 0.001305152864920274 0.0 78-79 16.084766946861272 4.669598801145882E-6 1.1673997002864705E-5 0.0015222892091735576 0.0 80-81 16.342436576108202 4.669598801145882E-6 1.1673997002864705E-5 0.001776782343836008 0.0 82-83 16.60152342859128 5.8369985014323524E-6 1.1673997002864705E-5 0.0018620025219569206 0.0 84-85 16.86385681964086 7.0043982017188234E-6 1.1673997002864705E-5 0.0019016941117666604 0.0 86-87 17.126933844299515 7.0043982017188234E-6 1.1673997002864705E-5 0.0019285443048732494 0.0 88 17.32407146428719 7.0043982017188234E-6 1.1673997002864705E-5 0.001937883502475541 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTTT 9335 0.0 52.770767 1 GCTTTCT 8480 0.0 46.007504 1 TGCCCTA 14855 0.0 43.480145 6 GCTTTTC 9560 0.0 40.514996 1 TGCTCTA 14220 0.0 39.86801 6 GCCCTAC 38275 0.0 38.758617 7 GACTTTT 10600 0.0 38.092 1 GATTTCT 11680 0.0 37.22591 1 GCTGCGT 2005 0.0 36.107624 5 CCCCTAC 102040 0.0 36.032455 7 GCCAATG 1150 0.0 35.966217 2 TTTTGAC 6855 0.0 34.89942 3 GCCGCGT 1885 0.0 34.66539 5 CGCCCTA 14955 0.0 33.853703 6 TTGCCCT 6890 0.0 32.818478 5 TTTGACG 4165 0.0 32.500076 4 GCCCCTA 11950 0.0 31.784863 6 CCCCCTA 64565 0.0 31.68609 6 CCTTTTG 3005 0.0 31.128277 1 CCGCGTG 1565 0.0 30.938538 6 >>END_MODULE