##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248445_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27712173
Sequences flagged as poor quality	0
Sequence length	100
%GC	23
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.232959176460106	33.0	31.0	34.0	23.0	34.0
2	30.662307643648155	33.0	31.0	34.0	26.0	34.0
3	31.10371254538574	34.0	31.0	34.0	27.0	34.0
4	34.517308476675574	37.0	35.0	37.0	31.0	37.0
5	34.38308403314313	37.0	35.0	37.0	30.0	37.0
6	34.192233355356144	37.0	35.0	37.0	29.0	37.0
7	34.385528626715775	37.0	35.0	37.0	30.0	37.0
8	34.370778033176975	37.0	35.0	37.0	30.0	37.0
9	35.772778590838044	39.0	37.0	39.0	30.0	39.0
10-11	36.4129402086224	39.0	37.0	39.0	31.0	39.0
12-13	36.50975477455341	39.0	37.0	39.0	32.0	39.0
14-15	37.82555758077867	40.0	38.0	41.0	32.0	41.0
16-17	37.7140976819104	40.0	38.0	41.0	32.0	41.0
18-19	37.233183770900965	40.0	36.0	41.0	31.0	41.0
20-21	36.92353813250227	39.5	35.0	41.0	30.5	41.0
22-23	36.678254913463476	39.0	35.0	41.0	30.0	41.0
24-25	36.521312655633324	39.0	35.0	41.0	30.0	41.0
26-27	36.40419509866656	39.0	35.0	41.0	28.0	41.0
28-29	36.30034625938572	39.0	35.0	41.0	27.5	41.0
30-31	36.212036439726326	39.0	35.0	41.0	27.0	41.0
32-33	36.13456737946895	39.0	35.0	41.0	26.5	41.0
34-35	36.05697016253471	39.0	35.0	41.0	25.5	41.0
36-37	35.947160278625574	39.0	35.0	41.0	25.0	41.0
38-39	35.84033231533305	38.5	35.0	41.0	25.0	41.0
40-41	35.748305212297865	38.0	35.0	41.0	25.0	41.0
42-43	35.65581304288192	38.0	35.0	40.5	25.0	41.0
44-45	35.526238000174146	38.0	35.0	40.0	25.0	41.0
46-47	35.41226642529982	38.0	35.0	40.0	25.0	41.0
48-49	35.28014851090891	38.0	34.5	40.0	25.0	41.0
50-51	35.11355895836822	37.5	34.0	40.0	25.0	41.0
52-53	34.920613280669116	37.0	34.0	40.0	25.0	41.0
54-55	34.74352328848409	37.0	33.5	40.0	25.0	41.0
56-57	34.536901797632396	36.0	33.0	40.0	25.0	41.0
58-59	34.600575187662116	36.0	33.0	40.0	26.0	41.0
60-61	34.419376459579695	35.5	33.0	39.0	26.0	41.0
62-63	34.18071186622572	35.0	33.0	39.0	25.5	41.0
64-65	33.939374259824376	35.0	33.0	39.0	25.0	41.0
66-67	33.68864565402359	35.0	33.0	39.0	25.0	40.5
68-69	33.44412018862613	35.0	32.0	38.0	25.0	40.0
70-71	33.21716084841127	35.0	32.0	37.0	25.0	40.0
72-73	33.11021483230492	35.0	32.0	37.0	25.0	39.5
74-75	33.06318005087512	35.0	32.0	36.5	25.0	39.0
76-77	32.88153020335143	35.0	32.0	36.0	25.0	39.0
78-79	32.6466477385227	35.0	32.0	36.0	25.0	39.0
80-81	32.42018549032586	35.0	32.0	35.5	25.0	37.5
82-83	32.207797724848206	35.0	32.0	35.0	25.0	37.0
84-85	31.996951267589157	35.0	32.0	35.0	24.0	37.0
86-87	31.799637852289678	35.0	31.0	35.0	24.0	36.0
88-89	31.626551768423212	35.0	31.0	35.0	24.0	36.0
90-91	31.44406514061528	35.0	31.0	35.0	23.0	36.0
92-93	31.28395328291289	35.0	31.0	35.0	23.0	36.0
94-95	31.13136041334615	35.0	31.0	35.0	21.5	35.5
96-97	30.973363149833105	35.0	30.0	35.0	20.0	35.0
98-99	30.838333049523037	35.0	30.0	35.0	20.0	35.0
100	30.12869196507975	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2930911181104179
1101	2	0.24591072478891718
1101	3	0.24202659315384167
1101	4	0.22336413334362248
1101	5	0.1259979197570118
1101	6	0.15929172699055272
1101	7	0.1696593501183017
1101	8	0.19529897024833787
1101	9	0.3028389224265027
1101	10-11	0.3012168825957744
1101	12-13	0.2846507499761515
1101	14-15	0.43359038465187894
1101	16-17	0.4304257196267969
1101	18-19	0.4442871155792716
1101	20-21	0.4561961037049471
1101	22-23	0.46077074055313005
1101	24-25	0.4536131612833856
1101	26-27	0.4848580832412992
1101	28-29	0.47814847703637753
1101	30-31	0.45525960892653927
1101	32-33	0.47024082413479107
1101	34-35	0.48578659315920447
1101	36-37	0.47193960751267383
1101	38-39	0.48028579142136607
1101	40-41	0.5131279973639309
1101	42-43	0.5104801211323675
1101	44-45	0.48414416358590273
1101	46-47	0.493361875949887
1101	48-49	0.517807503363116
1101	50-51	0.5122199298409811
1101	52-53	0.48384050382651367
1101	54-55	0.4793826444387079
1101	56-57	0.4930554486616643
1101	58-59	0.4790464152801377
1101	60-61	0.4457640409223913
1101	62-63	0.46211334445990104
1101	64-65	0.4560790108020143
1101	66-67	0.4243104378745741
1101	68-69	0.4139548588134332
1101	70-71	0.4062839784329171
1101	72-73	0.39266869223835243
1101	74-75	0.39931644041417513
1101	76-77	0.4089585711647814
1101	78-79	0.3932031815728081
1101	80-81	0.38234898028926523
1101	82-83	0.32533111596410436
1101	84-85	0.3194552169280769
1101	86-87	0.27166739140093554
1101	88-89	0.3014544601387499
1101	90-91	0.318968821908598
1101	92-93	0.32011544663149394
1101	94-95	0.29682834444261275
1101	96-97	0.29968271104463184
1101	98-99	0.3435633407932457
1101	100	0.33568144624844365
1102	1	0.16838790002158888
1102	2	0.15324951506864082
1102	3	0.20778835538493823
1102	4	0.16556420614315215
1102	5	0.13059257339575936
1102	6	-0.006900251623065401
1102	7	0.07830872064472061
1102	8	0.15150482961765732
1102	9	0.19343644549767447
1102	10-11	0.2482211436337991
1102	12-13	0.25708810913629065
1102	14-15	0.3678304464331603
1102	16-17	0.4075118107487157
1102	18-19	0.35585088588223357
1102	20-21	0.3505376865249801
1102	22-23	0.34521158805390684
1102	24-25	0.27078393184542193
1102	26-27	0.3120818153257474
1102	28-29	0.3417447512598031
1102	30-31	0.3486965707653198
1102	32-33	0.3428011144082248
1102	34-35	0.3598364055408467
1102	36-37	0.3677249183600395
1102	38-39	0.34238725014920135
1102	40-41	0.3372387710422089
1102	42-43	0.3555127196479546
1102	44-45	0.32282243561081003
1102	46-47	0.34099532862914117
1102	48-49	0.34295911474501395
1102	50-51	0.31003566121548687
1102	52-53	0.31240994289785107
1102	54-55	0.3033058288469519
1102	56-57	0.3207388824366788
1102	58-59	0.32536586345459995
1102	60-61	0.312023857856758
1102	62-63	0.3311533899964516
1102	64-65	0.3165234277414726
1102	66-67	0.31063485745369945
1102	68-69	0.3173166381572301
1102	70-71	0.3076590733050679
1102	72-73	0.31246186161260425
1102	74-75	0.2878066949715219
1102	76-77	0.2986960906600231
1102	78-79	0.2832240509880677
1102	80-81	0.26584640791536174
1102	82-83	0.2849106322419317
1102	84-85	0.24454484593329795
1102	86-87	0.2164087028901669
1102	88-89	0.19959305599994082
1102	90-91	0.24832578919141568
1102	92-93	0.21178303176339597
1102	94-95	0.2029842989687083
1102	96-97	0.24743297240584283
1102	98-99	0.22246238561149312
1102	100	0.19567030217236692
1103	1	-2.3488448892194924E-4
1103	2	-0.007688568456135414
1103	3	0.07933695506434546
1103	4	-0.00698461580753218
1103	5	-0.04079987936426477
1103	6	-0.08655849854692832
1103	7	-0.03179490128149354
1103	8	-0.011687524373996894
1103	9	-0.049721375824148595
1103	10-11	0.061739244238204094
1103	12-13	0.06931945580471677
1103	14-15	0.13938503763878884
1103	16-17	0.19329674813202047
1103	18-19	0.14571921889166362
1103	20-21	0.14744537775137445
1103	22-23	0.14846114391639986
1103	24-25	0.12974283434224532
1103	26-27	0.12379525372655564
1103	28-29	0.11486607411102057
1103	30-31	0.11933499774202971
1103	32-33	0.15609773839227614
1103	34-35	0.18153437367588765
1103	36-37	0.16471877924445977
1103	38-39	0.14080902458682942
1103	40-41	0.1602283860129674
1103	42-43	0.19427497880361244
1103	44-45	0.13690166046809793
1103	46-47	0.17612201052484266
1103	48-49	0.17260125799557358
1103	50-51	0.18769356852180152
1103	52-53	0.19773992392879336
1103	54-55	0.14436112208571927
1103	56-57	0.1568013850260499
1103	58-59	0.15750734108717523
1103	60-61	0.15952016445810813
1103	62-63	0.1729588784844438
1103	64-65	0.16761694994187337
1103	66-67	0.16460791826799692
1103	68-69	0.16428203119124163
1103	70-71	0.15962661691587954
1103	72-73	0.19465783312515583
1103	74-75	0.15105808470703863
1103	76-77	0.1610485061222917
1103	78-79	0.1538662359771763
1103	80-81	0.1361745346283456
1103	82-83	0.1497921437544818
1103	84-85	0.13644862831761628
1103	86-87	0.11704016624136315
1103	88-89	0.14204924151783516
1103	90-91	0.125154927080537
1103	92-93	0.13130184184598193
1103	94-95	0.13889405775770314
1103	96-97	0.16752550854267056
1103	98-99	0.1724805978144417
1103	100	0.1418831028837566
1104	1	0.010478462555585821
1104	2	0.024939063475251544
1104	3	0.11643510187526829
1104	4	0.08755451486839405
1104	5	0.07327818447334522
1104	6	-0.04135053121975574
1104	7	0.014861124892355804
1104	8	0.05236888944772744
1104	9	0.12052616981770825
1104	10-11	0.09736016942378001
1104	12-13	0.12751433599012074
1104	14-15	0.16898197499095602
1104	16-17	0.22164999790564366
1104	18-19	0.2232426452718954
1104	20-21	0.20404515028140935
1104	22-23	0.24083790026752894
1104	24-25	0.23626801004144937
1104	26-27	0.22462564980290978
1104	28-29	0.2463108441831423
1104	30-31	0.23743313494805562
1104	32-33	0.25577691099610433
1104	34-35	0.22714256984515657
1104	36-37	0.24897548839148698
1104	38-39	0.22097090971182354
1104	40-41	0.2256918024402168
1104	42-43	0.24183195010656533
1104	44-45	0.22180750250596049
1104	46-47	0.25466750917535563
1104	48-49	0.24926488070423147
1104	50-51	0.25552397174766384
1104	52-53	0.22051698278363574
1104	54-55	0.21848157673965574
1104	56-57	0.20735371499716138
1104	58-59	0.1980463000304269
1104	60-61	0.1905178316235805
1104	62-63	0.20054914772704535
1104	64-65	0.2058343857477638
1104	66-67	0.14992984372778295
1104	68-69	0.1599887259417656
1104	70-71	0.16248797499200407
1104	72-73	0.17855468721803192
1104	74-75	0.17785139591443766
1104	76-77	0.1730500850807246
1104	78-79	0.18152542216218137
1104	80-81	0.16674352945708648
1104	82-83	0.16220884853381534
1104	84-85	0.10263883431232301
1104	86-87	0.10741231845255683
1104	88-89	0.1255292553517755
1104	90-91	0.1641926052143674
1104	92-93	0.0994988184987875
1104	94-95	0.12198753514427096
1104	96-97	0.15474716060952787
1104	98-99	0.1768855105243432
1104	100	0.17933823122359982
1105	1	0.06432220518601994
1105	2	0.0701229938584298
1105	3	0.09896492493261988
1105	4	0.08734715167635443
1105	5	0.04608325096576493
1105	6	-0.020786031019191853
1105	7	-0.0011037206293735835
1105	8	0.07169589827168465
1105	9	0.0572810073761687
1105	10-11	0.06687099826698528
1105	12-13	0.08910417063096077
1105	14-15	0.1330165124916718
1105	16-17	0.11979487501680808
1105	18-19	0.12208609719007057
1105	20-21	0.1410953498688272
1105	22-23	0.12150346915465349
1105	24-25	0.11418350582903969
1105	26-27	0.1299072716751013
1105	28-29	0.14367642038548922
1105	30-31	0.10373017590916334
1105	32-33	0.15364370430496166
1105	34-35	0.17149645060440122
1105	36-37	0.15278216750252938
1105	38-39	0.08978937295139389
1105	40-41	0.08354909350618556
1105	42-43	0.08925719896941331
1105	44-45	0.11883882758347397
1105	46-47	0.13553266819387488
1105	48-49	0.14914862740879187
1105	50-51	0.1364044206065813
1105	52-53	0.1257229599854952
1105	54-55	0.13399880660941932
1105	56-57	0.12603551628094323
1105	58-59	0.12485343738121912
1105	60-61	0.11175967372522422
1105	62-63	0.1503887468188907
1105	64-65	0.15438821544524473
1105	66-67	0.1437973183087493
1105	68-69	0.13099857401430626
1105	70-71	0.12611156200311058
1105	72-73	0.13795018503710565
1105	74-75	0.10905015040017929
1105	76-77	0.12860469300878918
1105	78-79	0.09516604811378215
1105	80-81	0.10222203990658585
1105	82-83	0.07077948386780974
1105	84-85	0.09295919066213543
1105	86-87	0.10534514895733693
1105	88-89	0.08734204859073458
1105	90-91	0.10307384816337262
1105	92-93	0.08056471411481425
1105	94-95	0.07564016326709222
1105	96-97	0.0883148536417444
1105	98-99	0.09577792881579228
1105	100	0.13630949058593345
1106	1	0.006437195513093741
1106	2	-0.006997701187028582
1106	3	0.061277777688861335
1106	4	0.04044596227978303
1106	5	0.022168684056040888
1106	6	0.019446559368333283
1106	7	0.008355192295631753
1106	8	0.03937676226231446
1106	9	0.029454706766941285
1106	10-11	0.047111881082535945
1106	12-13	0.06749266078190175
1106	14-15	0.09680633239297975
1106	16-17	0.10757383992740444
1106	18-19	0.12110512606258794
1106	20-21	0.09211559011841786
1106	22-23	0.05823069075853482
1106	24-25	0.07463294146389643
1106	26-27	0.0748126246375378
1106	28-29	0.10221223501540777
1106	30-31	0.09919970526117083
1106	32-33	0.10724690356753541
1106	34-35	0.10252628431568667
1106	36-37	0.11190283006408919
1106	38-39	0.09161671158700813
1106	40-41	0.09962206617588976
1106	42-43	0.0815521529301364
1106	44-45	0.10293081652317682
1106	46-47	0.1076214532300881
1106	48-49	0.09713547042348836
1106	50-51	0.1141018084088401
1106	52-53	0.11826600826454126
1106	54-55	0.1519724009172947
1106	56-57	0.09807916295997643
1106	58-59	0.08680907566926521
1106	60-61	0.09908126064275535
1106	62-63	0.08388926973285749
1106	64-65	0.0878284237494995
1106	66-67	0.07322364867101072
1106	68-69	0.0842459487633036
1106	70-71	0.0875330018158138
1106	72-73	0.09647684267947199
1106	74-75	0.0817576046294235
1106	76-77	0.11710027342090257
1106	78-79	0.15740240303274788
1106	80-81	0.15303140519632308
1106	82-83	0.14725856159509476
1106	84-85	0.1461836550354647
1106	86-87	0.10512093069115025
1106	88-89	0.09984433816026694
1106	90-91	0.1270346100926183
1106	92-93	0.10997511904286483
1106	94-95	0.14660766919297075
1106	96-97	0.16345795944064534
1106	98-99	0.15133192004502405
1106	100	0.10593556639807034
1107	1	-0.02443225332518395
1107	2	0.05178374997531421
1107	3	0.06722129868805027
1107	4	0.03187751164249164
1107	5	0.030175381316695393
1107	6	0.01733622553658165
1107	7	-0.011090671608180003
1107	8	0.04146138562350643
1107	9	-0.0161413757318698
1107	10-11	0.04090910415547455
1107	12-13	0.08196886047947771
1107	14-15	0.09792326825851916
1107	16-17	0.10688673606760091
1107	18-19	0.0877709061912384
1107	20-21	0.08088233516946275
1107	22-23	0.06500281654825812
1107	24-25	0.06169782441956073
1107	26-27	0.08584764152565327
1107	28-29	0.05987925276328809
1107	30-31	0.09524608003836477
1107	32-33	0.10347758664455142
1107	34-35	0.10059243319727074
1107	36-37	0.09497985709699464
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2216	46-47	-0.13917730182916443
2216	48-49	-0.107071263240897
2216	50-51	-0.11722817959182663
2216	52-53	-0.14372115075324388
2216	54-55	-0.17460534076180778
2216	56-57	-0.18122534713972271
2216	58-59	-0.20501255259656403
2216	60-61	-0.21101371137481095
2216	62-63	-0.2257503566232799
2216	64-65	-0.23603254721592748
2216	66-67	-0.23982220715607383
2216	68-69	-0.2413735886888233
2216	70-71	-0.27152313526454463
2216	72-73	-0.26082777192210926
2216	74-75	-0.26153332551691477
2216	76-77	-0.23199277292009413
2216	78-79	-0.227929765193835
2216	80-81	-0.2657333233398873
2216	82-83	-0.25314364074475293
2216	84-85	-0.2453827032996898
2216	86-87	-0.25426328056240166
2216	88-89	-0.2327598976706753
2216	90-91	-0.23473560178177166
2216	92-93	-0.27735224225182264
2216	94-95	-0.26631348979246994
2216	96-97	-0.20318046712954896
2216	98-99	-0.22986683965461907
2216	100	-0.1470767037433447
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	32094.0
1	1558.0
2	935.0
3	808.0
4	670.0
5	1413.0
6	7023.0
7	19592.0
8	28052.0
9	36016.0
10	48700.0
11	61940.0
12	71520.0
13	78496.0
14	88311.0
15	101072.0
16	114854.0
17	128160.0
18	141257.0
19	152451.0
20	165823.0
21	177288.0
22	193268.0
23	217429.0
24	303854.0
25	388266.0
26	263073.0
27	557186.0
28	334367.0
29	574263.0
30	472104.0
31	572214.0
32	742476.0
33	1313903.0
34	1563568.0
35	5105430.0
36	3170210.0
37	4572375.0
38	4390748.0
39	1506307.0
40	13099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.781734124335998	31.39008704190525	29.191421642045658	23.63675719171309
2	12.399975440283335	34.7839274362283	31.780786531792526	21.03531059169583
3	11.014384378189728	36.858471145015706	31.08967877100423	21.03746570579034
4	9.95749283431276	36.585517280134056	31.77718620090016	21.679803684653027
5	10.07534626759525	36.01067351251652	32.187860469553605	21.726119750334632
6	9.593851861042596	33.86564081581981	33.7080683972185	22.832438925919103
7	12.144522798939587	39.42305548711566	28.54607324888507	19.886348465059683
8	13.505740761744342	35.012545693419696	24.92715447529308	26.554559069542883
9	9.930863326077278	34.0638636945528	27.901419537671977	28.103853441697947
10-11	11.492798396435134	50.64598688207861	26.263554178558895	11.59766054292736
12-13	10.603470094459865	50.57756648948158	27.510893875272924	11.308069540785622
14-15	10.823606402880479	49.81559394894043	27.353660603393102	12.00713904478598
16-17	10.870163425865679	49.71676719864269	27.43712919072107	11.975940184770561
18-19	10.581027138579866	49.442045183091835	28.06603855829139	11.910889120036908
20-21	10.602635858722731	49.2105564946985	28.067589888579498	12.119217757999271
22-23	10.835293707176024	49.336136172403116	28.192583469266257	11.635986651154603
24-25	10.556548531777334	48.99336507263613	28.491623997980227	11.95846239760631
26-27	10.951781485181947	48.8822406985323	28.16809922394294	11.997878592342811
28-29	11.044492909595604	49.13139280236492	28.164955720590513	11.65915856744896
30-31	10.89756028019471	49.13677519873716	28.434167189371916	11.531497331696212
32-33	10.99834561214284	49.03890223608871	28.297671718582702	11.665080433185747
34-35	11.094495979079424	49.24424046533564	28.196480349720876	11.464783205864059
36-37	11.039804498888275	49.0957489953123	28.345147798062047	11.519298707737377
38-39	11.215317066644724	49.03075325162297	28.366367692731508	11.387561989000798
40-41	11.355899848722405	49.42081505602782	28.223462617179152	10.999822478070621
42-43	11.188755860493343	49.39336089693429	28.479612221607653	10.938271020964715
44-45	11.356817883534315	49.2257020590417	28.277980531235418	11.13949952618856
46-47	11.466672438796124	49.24147569885919	28.24614617308359	11.045705689261096
48-49	11.233332489542551	49.160241163717224	28.54127202056797	11.065154326172259
50-51	11.441390620320991	49.05490866827907	28.48932386958582	11.014376841814123
52-53	11.642510482603868	49.06517366338952	28.538712978662485	10.753602875344125
54-55	11.44557180808848	48.97403568381266	28.688195285617706	10.892197222481153
56-57	11.591479113342588	48.885426778607965	28.41893769184382	11.104156416205626
58-59	11.644528572060691	48.815129629545645	28.432474889199614	11.107866909194048
60-61	11.412243194385848	48.79845010962882	28.76946871355305	11.019837982432287
62-63	11.553013395770892	48.540486118048186	28.686703365968402	11.219797120212517
64-65	11.725974105510641	48.594100166765756	28.519849569373633	11.160076158349968
66-67	11.44448381403629	48.46817952488115	28.945033784933045	11.142302876149516
68-69	11.488932487870704	48.558851959264224	28.730519427378624	11.221696125486446
70-71	11.483702860510636	48.88051607847748	28.595566092986598	11.040214968025287
72-73	11.255654002617344	48.920856979805066	28.8519350236987	10.97155399387889
74-75	11.378502932555797	48.98174199994728	28.617806881815007	11.021948185681916
76-77	11.452839617939151	49.25858920336352	28.465280599410438	10.823290579286898
78-79	11.077449351973852	49.47392032705103	28.66352734056029	10.785102980414823
80-81	11.090040747858133	49.60063348744729	28.467528231417443	10.841797533277141
82-83	11.15342125699955	49.732565951827354	28.39738991146989	10.716622879703209
84-85	10.920748976507424	49.9452635552319	28.42637661860532	10.707610849655365
86-87	11.045973608228502	50.06759352979421	28.1108568573281	10.775576004649189
88-89	11.206434216585642	50.243824663309454	27.963189000857934	10.586552119246974
90-91	11.036537505681483	50.25135463586869	28.196986808337105	10.515121050112725
92-93	11.215532151890208	50.265258087003836	27.927896321913863	10.591313439192085
94-95	11.473176337502263	50.24372912397506	27.72522007449922	10.557874464023463
96-97	11.413012628295618	50.01695510948231	27.98821564789132	10.581816614330751
98-99	11.574266209987329	49.90394489090939	27.86461397640184	10.657174922701442
100	11.83858568836281	49.95394205128478	27.775524835321107	10.431947425031307
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2693125.0
1	2260101.5
2	1085921.5
3	228481.0
4	111821.5
5	106978.5
6	94017.5
7	78699.5
8	71061.0
9	76068.5
10	93788.5
11	126392.5
12	175419.0
13	239107.0
14	314178.5
15	393055.0
16	466796.0
17	533476.0
18	595375.0
19	654098.5
20	712511.0
21	772135.0
22	835933.5
23	900996.0
24	951870.5
25	977012.5
26	980259.0
27	972154.5
28	957287.5
29	927001.0
30	874506.0
31	803861.0
32	731835.0
33	681974.5
34	638956.0
35	578590.5
36	510886.0
37	449943.0
38	399730.5
39	357243.5
40	321368.5
41	291587.5
42	267964.0
43	252368.0
44	244741.0
45	243096.0
46	243099.0
47	242139.0
48	237111.5
49	225313.5
50	206054.5
51	180012.0
52	151320.5
53	122803.0
54	96480.0
55	73972.5
56	55356.0
57	40462.0
58	28793.5
59	20574.5
60	14959.5
61	11118.0
62	8756.0
63	7188.5
64	6069.0
65	5205.0
66	4522.5
67	3964.5
68	3509.0
69	3111.0
70	2726.0
71	2406.0
72	2185.5
73	1955.5
74	1780.0
75	1645.0
76	1460.5
77	1365.0
78	1312.5
79	1204.0
80	1103.5
81	1024.0
82	962.5
83	940.5
84	901.0
85	852.5
86	822.0
87	792.5
88	811.5
89	747.0
90	668.0
91	666.0
92	634.0
93	592.0
94	553.0
95	533.5
96	519.5
97	466.5
98	418.0
99	376.5
100	408.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1575517011964381
2	0.14738649329303768
3	0.14910775852907673
4	0.14566883657950605
5	0.14409912928877863
6	0.12765148369996104
7	0.12925727621576266
8	0.1304192204631517
9	0.12983103129444235
10-11	0.1293186210983888
12-13	0.13306246319983642
14-15	0.1407432033568786
16-17	0.1340475898443619
18-19	0.14141258428200487
20-21	0.1424770984216936
22-23	0.14259978818694585
24-25	0.14344779097618943
26-27	0.14427233836913475
28-29	0.1445429775571912
30-31	0.14300755123028425
32-33	0.14326556058956474
34-35	0.14533685250882347
36-37	0.146197485126843
38-39	0.146253417225708
40-41	0.14266474159207942
42-43	0.14016042697193035
44-45	0.13971116591975663
46-47	0.14060968802410406
48-49	0.1423074978638449
50-51	0.1396859062622047
52-53	0.14009908208930422
54-55	0.1428884699875394
56-57	0.13936474775904437
58-59	0.14026868264715292
60-61	0.14162729137119634
62-63	0.13641838913173646
64-65	0.12856443989433813
66-67	0.1288549259561854
68-69	0.12693338772098456
70-71	0.1254123954841073
72-73	0.12864924017326249
74-75	0.1301179088337822
76-77	0.12766050500622958
78-79	0.12285575728760065
80-81	0.12368752172556081
82-83	0.12418549783158468
84-85	0.11970912566113094
86-87	0.12351250838395099
88-89	0.12300370671040485
90-91	0.12860593790317346
92-93	0.12519588413366214
94-95	0.12715892037769827
96-97	0.13044808864321106
98-99	0.1341450199520622
100	0.13443189749140205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7712173E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.207222132862775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05495388368858	41.0621602084653
2	18.221759282765113	20.48388943315866
3	5.714071044321691	9.635161814134454
4	1.7502623929150252	3.93509548437483
5	0.5548658915272477	1.5593735209510475
6	0.2145580146033806	0.7235825992318936
7	0.10856699740737083	0.4271574537701821
8	0.06826710340252848	0.3069683396250424
9	0.056212927514789825	0.2843615253206075
>10	0.23198497249573777	2.2767820554195026
>50	0.014503267778187485	0.5592288084482794
>100	0.008265351010721686	0.8588766017664254
>500	7.342542589013066E-4	0.2995618285965196
>1k	6.61602773817045E-4	0.7251882448878886
>5k	9.606159723123054E-5	0.3631210579430932
>10k+	2.3695193983451328E-4	16.499491023906323
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1913518	6.904972771352141	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	451727	1.6300670467090401	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	353111	1.2742089911173693	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	352253	1.2711128788060033	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	193438	0.6980253767901925	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125987	0.45462692514224706	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115003	0.41499091392075244	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	109960	0.39679313491583645	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97998	0.35362798868208567	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	88005	0.31756802326544364	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71831	0.2592037802304424	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57017	0.20574712780553153	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48927	0.17655418072050866	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47756	0.17232860086432053	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44097	0.15912501700967296	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43918	0.1584790914808449	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42969	0.15505460362130388	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42219	0.15234821174073934	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33361	0.12038391937001837	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32638	0.11777495759715413	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	30680	0.11070947052762697	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30189	0.10893768597648404	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29107	0.10503326462345627	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29085	0.10495387712829303	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.09347671869687E-4	0.0	0.0	3.608522507419393E-6	0.0
2	0.0021037686218255062	0.0	0.0	7.217045014838786E-6	0.0
3	0.0034858327421671336	0.0	0.0	2.8868180059355144E-5	0.0
4	0.005611252499037156	0.0	0.0	3.969374758161332E-5	0.0
5	0.009533716464602037	0.0	0.0	4.691079259645211E-5	0.0
6	0.01711522225269018	0.0	0.0	5.05193151038715E-5	3.608522507419393E-6
7	0.0259019745582564	0.0	0.0	5.05193151038715E-5	3.608522507419393E-6
8	0.04924550665875246	0.0	0.0	5.05193151038715E-5	3.608522507419393E-6
9	0.1319239743487456	0.0	0.0	5.05193151038715E-5	3.608522507419393E-6
10-11	0.30399997863754674	0.0	0.0	5.05193151038715E-5	3.608522507419393E-6
12-13	0.5011732569654498	0.0	0.0	5.412783761129089E-5	3.608522507419393E-6
14-15	0.7164955992444186	0.0	0.0	7.036618889467816E-5	3.608522507419393E-6
16-17	0.9485163794264708	0.0	0.0	8.660454017806543E-5	3.608522507419393E-6
18-19	1.19207721458725	0.0	0.0	1.0825567522258179E-4	3.608522507419393E-6
20-21	1.5091869554942516	0.0	0.0	1.1366845898371088E-4	3.608522507419393E-6
22-23	1.8670441325550327	0.0	0.0	1.1547272023742058E-4	3.608522507419393E-6
24-25	2.2258846319990857	0.0	0.0	1.1547272023742058E-4	3.608522507419393E-6
26-27	2.5518911851481296	0.0	0.0	1.3171107152080784E-4	3.608522507419393E-6
28-29	2.8609629421698544	0.0	0.0	1.4434090029677573E-4	3.608522507419393E-6
30-31	3.190783703609241	0.0	0.0	1.515579453116145E-4	3.608522507419393E-6
32-33	3.5632084860324738	0.0	0.0	1.551664678190339E-4	3.608522507419393E-6
34-35	3.9165748568327716	0.0	0.0	1.6779629659500176E-4	3.608522507419393E-6
36-37	4.181220289004402	0.0	0.0	1.7862186411725996E-4	3.608522507419393E-6
38-39	4.377935284973863	0.0	0.0	2.3996674674338963E-4	3.608522507419393E-6
40-41	4.549823646092278	0.0	0.0	2.6161788178790594E-4	3.608522507419393E-6
42-43	4.69813572540847	0.0	0.0	3.500266832196811E-4	3.608522507419393E-6
44-45	4.828352868611205	0.0	0.0	4.0235025957726235E-4	3.608522507419393E-6
46-47	4.948792720080089	0.0	0.0	4.149800883532302E-4	3.608522507419393E-6
48-49	5.067641213123201	0.0	0.0	4.6008661969597256E-4	3.608522507419393E-6
50-51	5.192357163763376	0.0	0.0	5.484954211277477E-4	3.608522507419393E-6
52-53	5.322372229705696	0.0	0.0	6.549468350966199E-4	3.608522507419393E-6
54-55	5.450990797437646	0.0	0.0	8.010919966471052E-4	3.608522507419393E-6
56-57	5.5840027413223785	0.0	0.0	8.750667080492028E-4	3.608522507419393E-6
58-59	5.729077975949414	0.0	0.0	9.165647168845258E-4	3.608522507419393E-6
60-61	5.883847506292632	0.0	0.0	9.68888293242107E-4	3.608522507419393E-6
62-63	6.03193946573587	3.608522507419393E-6	0.0	0.001028428914614527	3.608522507419393E-6
64-65	6.1766231756708505	3.608522507419393E-6	0.0	0.0011042078872703343	3.608522507419393E-6
66-67	6.316882837011734	3.608522507419393E-6	0.0	0.0011908124274483997	3.608522507419393E-6
68-69	6.452864594920074	3.608522507419393E-6	0.0	0.0012449402650596907	3.608522507419393E-6
70-71	6.587882877318931	3.608522507419393E-6	0.0	0.001277416967626465	3.608522507419393E-6
72-73	6.723734728417003	3.608522507419393E-6	0.0	0.0013171107152080783	3.608522507419393E-6
74-75	6.865268198203006	3.608522507419393E-6	0.0	0.0013568044627896916	3.608522507419393E-6
76-77	7.010116095912075	3.608522507419393E-6	0.0	0.0014668643992659831	3.608522507419393E-6
78-79	7.158754746515187	3.608522507419393E-6	0.0	0.0016148138220701783	3.608522507419393E-6
80-81	7.309486340172602	3.608522507419393E-6	0.0	0.0018565848300672776	3.608522507419393E-6
82-83	7.464286542957133	3.608522507419393E-6	0.0	0.0019305595414693753	3.608522507419393E-6
84-85	7.622807132446813	3.608522507419393E-6	0.0	0.0019738618115584076	3.608522507419393E-6
86-87	7.787245698848661	3.608522507419393E-6	0.0	0.0020117512978863115	3.608522507419393E-6
88	7.914810578008444	3.608522507419393E-6	0.0	0.0020243811266622796	3.608522507419393E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGACG	4705	0.0	38.85785	8
CCGTTTT	3090	0.0	34.9927	1
CCGACGC	5285	0.0	33.526566	9
CTACCCG	4565	0.0	33.050987	5
TACCCGA	5320	0.0	30.741943	6
GGACCCA	580	0.0	29.179823	1
CCTTGTT	2480	0.0	29.003328	1
CTGCCAA	2195	0.0	28.908094	5
AGTTGCC	445	0.0	28.519299	3
CCGTGTT	925	0.0	28.461277	1
TGACGCC	515	0.0	28.293686	3
TGCCAAT	1390	0.0	27.724438	6
AGTGCCG	290	0.0	27.553442	4
CCGTGCC	495	0.0	27.537714	3
TGGGCCA	495	0.0	27.537066	4
CCTGTTT	2200	0.0	27.138708	1
GCTTACC	1005	0.0	27.126705	3
GCGACGC	1460	0.0	27.040533	8
TGCCGGA	400	0.0	27.022875	6
GCTGCCA	630	0.0	26.858816	4
>>END_MODULE