##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248445_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27712173
Sequences flagged as poor quality	0
Sequence length	100
%GC	23
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81906871756322	34.0	31.0	34.0	31.0	34.0
2	33.00675291684993	34.0	33.0	34.0	31.0	34.0
3	33.08757822780624	34.0	33.0	34.0	31.0	34.0
4	36.42523099866618	37.0	37.0	37.0	35.0	37.0
5	36.33733042154435	37.0	37.0	37.0	35.0	37.0
6	36.26076305889112	37.0	37.0	37.0	35.0	37.0
7	36.297724324974446	37.0	37.0	37.0	35.0	37.0
8	36.06494117224225	37.0	36.0	37.0	35.0	37.0
9	37.849329498628634	39.0	38.0	39.0	35.0	39.0
10-11	38.05965737511815	39.0	39.0	39.0	36.0	39.0
12-13	38.14122703405467	39.0	39.0	39.0	37.0	39.0
14-15	39.73494557788737	41.0	40.0	41.0	37.0	41.0
16-17	39.737692349134804	41.0	40.0	41.0	37.0	41.0
18-19	39.6117787118318	41.0	39.5	41.0	37.0	41.0
20-21	39.30223001639027	41.0	39.0	41.0	35.5	41.0
22-23	38.88462036160065	40.5	38.0	41.0	35.0	41.0
24-25	38.52944301769479	40.0	36.5	41.0	35.0	41.0
26-27	38.380361384868664	40.0	35.0	41.0	35.0	41.0
28-29	38.28022719474218	40.0	35.0	41.0	35.0	41.0
30-31	38.20714748713499	40.0	35.0	41.0	35.0	41.0
32-33	38.140177711794735	40.0	35.0	41.0	35.0	41.0
34-35	38.07682903466285	40.0	35.0	41.0	35.0	41.0
36-37	38.01279894218328	40.0	35.0	41.0	35.0	41.0
38-39	37.926092804054015	40.0	35.0	41.0	35.0	41.0
40-41	37.83860538471667	40.0	35.0	41.0	34.0	41.0
42-43	37.73614342693372	40.0	35.0	41.0	34.0	41.0
44-45	37.64755768160079	40.0	35.0	41.0	34.0	41.0
46-47	37.564856065238914	40.0	35.0	41.0	33.5	41.0
48-49	37.49565523064539	39.5	35.0	41.0	33.5	41.0
50-51	37.33453065914391	39.0	35.0	41.0	33.0	41.0
52-53	37.11371677710008	39.0	35.0	41.0	33.0	41.0
54-55	36.97565477813667	39.0	35.0	41.0	33.0	41.0
56-57	36.78743608449615	38.0	35.0	41.0	33.0	41.0
58-59	36.57896268185104	37.5	35.0	40.5	33.0	41.0
60-61	36.3160021770938	37.0	35.0	40.0	32.5	41.0
62-63	36.02165869489917	36.5	35.0	40.0	32.0	41.0
64-65	35.7643319417788	36.0	35.0	39.5	31.5	41.0
66-67	35.512891013635056	35.0	35.0	39.0	31.0	41.0
68-69	35.276427564882766	35.0	35.0	39.0	31.0	41.0
70-71	35.10717927100123	35.0	35.0	38.5	31.0	40.5
72-73	34.91918892466499	35.0	35.0	37.5	31.0	40.0
74-75	34.67439043845461	35.0	35.0	37.0	31.0	39.5
76-77	34.44089245545631	35.0	35.0	36.5	31.0	39.0
78-79	34.21492166998236	35.0	34.0	36.0	31.0	39.0
80-81	34.00326354775571	35.0	34.0	36.0	30.0	38.5
82-83	33.81691015352712	35.0	34.0	35.5	30.0	37.0
84-85	33.63890711493465	35.0	34.0	35.0	30.0	37.0
86-87	33.479216696575904	35.0	34.0	35.0	30.0	36.5
88-89	33.335838676382394	35.0	34.0	35.0	29.5	36.0
90-91	33.217720043101636	35.0	34.0	35.0	29.0	36.0
92-93	33.08120171593906	35.0	34.0	35.0	29.0	36.0
94-95	32.9503498877551	35.0	34.0	35.0	29.0	36.0
96-97	32.851313698857176	35.0	34.0	35.0	29.0	35.5
98-99	32.73349830776533	35.0	34.0	35.0	29.0	35.0
100	32.15832082168367	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1734056494908529
1101	2	0.18123657166346163
1101	3	0.17408428720184332
1101	4	0.09297816445297968
1101	5	0.10900197837084136
1101	6	-0.009454175737438675
1101	7	0.05057676517942866
1101	8	-0.05646332444531765
1101	9	-0.08713575667726303
1101	10-11	0.022142405130857412
1101	12-13	0.1254831468437061
1101	14-15	0.17515606109051873
1101	16-17	0.20499635200532396
1101	18-19	0.2177830801974565
1101	20-21	0.23622100026428683
1101	22-23	0.19169643450206308
1101	24-25	0.19486076793742768
1101	26-27	0.21222454788659917
1101	28-29	0.2220447092122626
1101	30-31	0.2197894541996135
1101	32-33	0.2506863718239245
1101	34-35	0.2538528603673882
1101	36-37	0.25029050299635003
1101	38-39	0.23629705633227616
1101	40-41	0.23305454958407523
1101	42-43	0.24187749913232182
1101	44-45	0.25848071568808706
1101	46-47	0.2426096458918039
1101	48-49	0.24123666477149186
1101	50-51	0.25909737582264114
1101	52-53	0.20745993847675948
1101	54-55	0.24159537986182045
1101	56-57	0.24041273625650206
1101	58-59	0.24880506887154752
1101	60-61	0.21301122259773564
1101	62-63	0.17691014642849012
1101	64-65	0.182461919881014
1101	66-67	0.17845735949104125
1101	68-69	0.17913315286256193
1101	70-71	0.17326626381057508
1101	72-73	0.16950656592162971
1101	74-75	0.18703123974959368
1101	76-77	0.1713021001262831
1101	78-79	0.21103473062733258
1101	80-81	0.19909411683952527
1101	82-83	0.16857323916490685
1101	84-85	0.15723369329269588
1101	86-87	0.20038815039533375
1101	88-89	0.24087746940800514
1101	90-91	0.22439786134578554
1101	92-93	0.21566670420195067
1101	94-95	0.21698940023950541
1101	96-97	0.21915322355997802
1101	98-99	0.2214333593684259
1101	100	0.1587000767808675
1102	1	0.1698340615405627
1102	2	0.17506901680203413
1102	3	0.17291333636715933
1102	4	0.10715561179110011
1102	5	0.10996848100072754
1102	6	0.0047957346530154155
1102	7	0.023699224678850328
1102	8	-0.0508783107491837
1102	9	-0.02716151731137728
1102	10-11	0.0421036735161735
1102	12-13	0.11008088725611032
1102	14-15	0.1850821007185317
1102	16-17	0.21903217396245367
1102	18-19	0.23363429811375624
1102	20-21	0.22604869252270987
1102	22-23	0.2109267208776089
1102	24-25	0.1745913621846782
1102	26-27	0.17592538105403577
1102	28-29	0.1946305096300378
1102	30-31	0.2074070390618843
1102	32-33	0.20935424796983426
1102	34-35	0.2151555841232451
1102	36-37	0.20855973402269967
1102	38-39	0.1992406153190842
1102	40-41	0.19173856659518407
1102	42-43	0.16507480949262288
1102	44-45	0.20732214118313408
1102	46-47	0.2372204833972944
1102	48-49	0.22494829701108898
1102	50-51	0.2049180652716558
1102	52-53	0.18915192156323002
1102	54-55	0.21643316377407018
1102	56-57	0.17880874174574046
1102	58-59	0.18543957452172322
1102	60-61	0.18880277022220326
1102	62-63	0.1481606989681481
1102	64-65	0.16761904433496966
1102	66-67	0.16174366359221892
1102	68-69	0.15826485973952487
1102	70-71	0.1846044116155241
1102	72-73	0.15436855552098905
1102	74-75	0.14744675798353057
1102	76-77	0.1608329078036448
1102	78-79	0.15616295353321874
1102	80-81	0.15055899379214566
1102	82-83	0.156662912211182
1102	84-85	0.12503011803242714
1102	86-87	0.16086311388190921
1102	88-89	0.15887387503435946
1102	90-91	0.1636422044660577
1102	92-93	0.167154975018164
1102	94-95	0.1650854298623301
1102	96-97	0.16600392058746394
1102	98-99	0.1849347394179759
1102	100	0.1370544944242411
1103	1	0.12982215409582665
1103	2	0.14803915738379203
1103	3	0.14352236814161046
1103	4	0.07607081456753662
1103	5	0.08044966783211294
1103	6	0.017358505456925855
1103	7	0.01858380370469348
1103	8	-0.06595783271396272
1103	9	-0.14976300312655866
1103	10-11	-0.02579179942517129
1103	12-13	0.0825008998795198
1103	14-15	0.15618719244343282
1103	16-17	0.1779089795886577
1103	18-19	0.1598918956290163
1103	20-21	0.16339159954242177
1103	22-23	0.12117503129575624
1103	24-25	0.09910355774883328
1103	26-27	0.08671488691823015
1103	28-29	0.10374761897091389
1103	30-31	0.10391888142996919
1103	32-33	0.11311881971882087
1103	34-35	0.12503378085438754
1103	36-37	0.1108923634069896
1103	38-39	0.09178342259831851
1103	40-41	0.07790133196768778
1103	42-43	0.08565284441552024
1103	44-45	0.11200957384500043
1103	46-47	0.1021025426800719
1103	48-49	0.10668798672162438
1103	50-51	0.10939452770051616
1103	52-53	0.06791284061058178
1103	54-55	0.10934469834157312
1103	56-57	0.10779980375571796
1103	58-59	0.10606177669846772
1103	60-61	0.11465878426486853
1103	62-63	0.06855556955510878
1103	64-65	0.06340166411013115
1103	66-67	0.0536817063042605
1103	68-69	0.06149131358536408
1103	70-71	0.07760193222229361
1103	72-73	0.0666483451371036
1103	74-75	0.06486214889204689
1103	76-77	0.0335664054731879
1103	78-79	0.08117344950521499
1103	80-81	0.041885873826373654
1103	82-83	0.029620925468606174
1103	84-85	0.008840501888968788
1103	86-87	0.034249103879005816
1103	88-89	0.020763626014158376
1103	90-91	0.025683683942531843
1103	92-93	0.014873601497427558
1103	94-95	-0.007871365184968226
1103	96-97	0.017675513200096304
1103	98-99	0.005314577955374489
1103	100	-0.03345057056021261
1104	1	-0.2334669541024681
1104	2	-0.20846468605552104
1104	3	-0.15671651648284524
1104	4	-0.19833309215577088
1104	5	-0.11476554072089584
1104	6	-0.0535888038827963
1104	7	-0.0060848594806657275
1104	8	-0.09807952343872728
1104	9	0.010693620905200873
1104	10-11	0.07127945945114078
1104	12-13	0.09231087096155477
1104	14-15	0.12246768374908612
1104	16-17	0.13605213776405378
1104	18-19	0.1496087194296294
1104	20-21	0.14865284805050294
1104	22-23	0.13268541725879857
1104	24-25	0.12355160222428196
1104	26-27	0.14686425101265144
1104	28-29	0.13813616146966012
1104	30-31	0.15153317519235543
1104	32-33	0.15437243139485446
1104	34-35	0.16255217441295144
1104	36-37	0.14826968702479348
1104	38-39	0.1445670756122368
1104	40-41	0.15459979589909523
1104	42-43	0.15128230712696222
1104	44-45	0.162574640054153
1104	46-47	0.18085516624328335
1104	48-49	0.1784325350217273
1104	50-51	0.19926798137527157
1104	52-53	0.12611847394774856
1104	54-55	0.1714748825396697
1104	56-57	0.16689369436952006
1104	58-59	0.180101394949304
1104	60-61	0.15596095483440564
1104	62-63	0.11559410173040163
1104	64-65	0.09099076487727586
1104	66-67	0.09656882933641953
1104	68-69	0.08456621524530306
1104	70-71	0.11403007679520982
1104	72-73	0.10901674883858448
1104	74-75	0.09904802011723746
1104	76-77	0.08379230254350034
1104	78-79	0.11295078300233996
1104	80-81	0.0802990711572491
1104	82-83	0.08700805474756379
1104	84-85	0.06842731365747312
1104	86-87	0.07861605052480769
1104	88-89	0.06088845429050593
1104	90-91	0.061314752766094216
1104	92-93	0.0822713185505819
1104	94-95	0.10206827923018125
1104	96-97	0.07892944768732235
1104	98-99	0.09517132488648627
1104	100	0.11241425040631015
1105	1	0.09990241694689672
1105	2	0.09822487811924674
1105	3	0.09971921982238996
1105	4	0.052420942312188856
1105	5	0.06986100876816437
1105	6	0.01794853280687647
1105	7	0.04812493383885652
1105	8	-0.06462739269525741
1105	9	-0.05039075031986329
1105	10-11	0.02954994533394739
1105	12-13	0.07070694202727879
1105	14-15	0.11859448581078169
1105	16-17	0.1265511528262806
1105	18-19	0.1314260455773919
1105	20-21	0.13093952240193119
1105	22-23	0.10941728484353064
1105	24-25	0.0979682710543699
1105	26-27	0.11836565127502752
1105	28-29	0.13860004993860997
1105	30-31	0.1437021053325438
1105	32-33	0.14512189407000875
1105	34-35	0.14103158809285077
1105	36-37	0.1528306973321918
1105	38-39	0.13627475045068138
1105	40-41	0.11799339256261021
1105	42-43	0.09536255229083679
1105	44-45	0.11410252759255712
1105	46-47	0.1286302147315297
1105	48-49	0.09358084922675403
1105	50-51	0.09142623269174521
1105	52-53	-0.1568082068692931
1105	54-55	-0.09400529502723032
1105	56-57	-0.014555417656957559
1105	58-59	0.009987765915816738
1105	60-61	0.043088293270933775
1105	62-63	-0.016790069826015497
1105	64-65	0.0013342032302361417
1105	66-67	-0.005716102440892712
1105	68-69	-0.027874050488208013
1105	70-71	-0.027745606290750402
1105	72-73	-0.036592740724863404
1105	74-75	-0.022367264666883102
1105	76-77	-0.01952459115234717
1105	78-79	0.0025825826128098583
1105	80-81	-0.005186143042962499
1105	82-83	-0.011790630608956576
1105	84-85	-0.03443421647799738
1105	86-87	-0.030056280738129715
1105	88-89	-0.018864939522799773
1105	90-91	-0.012832698085027516
1105	92-93	-0.03233703798619558
1105	94-95	-0.059862245847824624
1105	96-97	-0.04299326680561677
1105	98-99	-0.05628835420619538
1105	100	-0.07302756870890903
1106	1	0.1228637879460095
1106	2	0.1072942339966545
1106	3	0.09211357263934872
1106	4	0.04879406477886761
1106	5	0.07238542513773183
1106	6	0.038028612390753835
1106	7	0.04522497353902821
1106	8	-0.027878786181233295
1106	9	-0.005806667560179335
1106	10-11	0.06209704777639047
1106	12-13	0.09652696121600712
1106	14-15	0.11661658327594182
1106	16-17	0.1081042202454654
1106	18-19	0.09413917203467292
1106	20-21	0.08906437829656966
1106	22-23	0.06742515289014506
1106	24-25	0.0738295078183242
1106	26-27	0.0950414266185291
1106	28-29	0.10062519434692518
1106	30-31	0.09566140254386113
1106	32-33	0.08611566981574725
1106	34-35	0.07641224888231335
1106	36-37	0.09725123134489166
1106	38-39	0.09416547158527777
1106	40-41	0.09402804391213948
1106	42-43	0.06720477202334507
1106	44-45	0.07620762568866724
1106	46-47	0.11557844621978575
1106	48-49	0.09234278972911625
1106	50-51	0.11315273999188236
1106	52-53	0.03738484435517364
1106	54-55	0.10171197502418039
1106	56-57	0.13657901665767724
1106	58-59	0.12223898347638595
1106	60-61	0.12871454788002978
1106	62-63	0.09601546602902289
1106	64-65	0.09477925946909949
1106	66-67	0.08591335460043581
1106	68-69	0.07883795298381813
1106	70-71	0.07706616078122153
1106	72-73	0.08547450872599427
1106	74-75	0.08886139056808418
1106	76-77	0.08788193309206349
1106	78-79	0.10419217459550367
1106	80-81	0.09079782084268118
1106	82-83	0.11351784139616683
1106	84-85	0.10056489369937793
1106	86-87	0.1201571063356397
1106	88-89	0.1016046951727958
1106	90-91	0.0963775565382079
1106	92-93	0.09747402749423628
1106	94-95	0.09214716651968757
1106	96-97	0.07774856991952817
1106	98-99	0.09454811068179936
1106	100	0.07975342496114024
1107	1	0.04806339380215263
1107	2	0.026509747366368686
1107	3	0.04820862434452522
1107	4	0.01875963749496634
1107	5	0.004617231087642892
1107	6	-0.01643900646983809
1107	7	-0.03325620746019098
1107	8	-0.110661291568384
1107	9	-0.08186865044249458
1107	10-11	-0.026232717393412486
1107	12-13	-0.0042413294675966995
1107	14-15	0.02027692548811899
1107	16-17	0.030567132761433413
1107	18-19	0.03800503754882811
1107	20-21	-4.417820007702744E-4
1107	22-23	-0.008479143881729101
1107	24-25	0.008848637893223099
1107	26-27	0.02533372011033208
1107	28-29	0.04125915776392475
1107	30-31	0.04227310463544853
1107	32-33	0.02531604656922326
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2216	24-25	-0.1832907199274274
2216	26-27	-0.18739615326177272
2216	28-29	-0.2121641981569482
2216	30-31	-0.1924651216888975
2216	32-33	-0.22027485093054366
2216	34-35	-0.21230318465157438
2216	36-37	-0.20520869375495465
2216	38-39	-0.19818750643268146
2216	40-41	-0.20270197787549904
2216	42-43	-0.20020005437627475
2216	44-45	-0.2504673695468611
2216	46-47	-0.2418466248168798
2216	48-49	-0.22717089793833622
2216	50-51	-0.24880215218357193
2216	52-53	-0.24133423751371197
2216	54-55	-0.2489827091468797
2216	56-57	-0.2599508496755121
2216	58-59	-0.25638877832214035
2216	60-61	-0.22911935177597798
2216	62-63	-0.21641265545599708
2216	64-65	-0.2366626607797926
2216	66-67	-0.22351176325427957
2216	68-69	-0.23372599181503517
2216	70-71	-0.21141848793858742
2216	72-73	-0.1826874555983906
2216	74-75	-0.15747387862103324
2216	76-77	-0.1972556410918216
2216	78-79	-0.20606779491006932
2216	80-81	-0.2027541871601315
2216	82-83	-0.20071266541755506
2216	84-85	-0.1804513096423932
2216	86-87	-0.2084239145741833
2216	88-89	-0.23721551951258846
2216	90-91	-0.23271050888716616
2216	92-93	-0.21977823671726782
2216	94-95	-0.24005807388890332
2216	96-97	-0.22900973063756425
2216	98-99	-0.21281841301654225
2216	100	-0.14713108847394096
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	25.0
7	194.0
8	811.0
9	1641.0
10	2937.0
11	4666.0
12	7181.0
13	10179.0
14	13142.0
15	16080.0
16	20176.0
17	25621.0
18	32064.0
19	40511.0
20	50693.0
21	65350.0
22	80852.0
23	95875.0
24	113857.0
25	136051.0
26	162430.0
27	194121.0
28	236238.0
29	296591.0
30	345647.0
31	429732.0
32	552570.0
33	794002.0
34	1127360.0
35	5330516.0
36	2640285.0
37	5643991.0
38	6574463.0
39	2635641.0
40	30678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.73957552554075	23.108174188191065	33.96676943330628	24.185480852961906
2	13.824698578668334	25.556768826834837	38.50904516592788	22.109487428568954
3	11.405029291500972	21.517364920363043	43.55427481694481	23.523330971191175
4	11.728327744468164	20.25278217514081	46.94298145014487	21.075908630246158
5	9.861459154946859	20.043697884777686	49.312305079662565	20.78253788061289
6	9.130149216673813	16.610055416737037	46.65053532943294	27.609260037156208
7	11.861531031868196	18.331521674608485	44.045131357977596	25.761815935545723
8	5.637309640063232	18.609370690634762	47.710567482384	28.042752186918
9	8.537396183258526	16.785948182410667	47.56221751358149	27.11443812074932
10-11	12.139151101116925	30.385167982057524	45.85047472480152	11.625206192024029
12-13	10.933281300488165	31.381074358924575	45.85057426549497	11.835070075092286
14-15	10.798552315283343	30.18452224473767	46.40074899406697	12.616176445912009
16-17	11.512297266237118	30.486719084536112	46.64216619053014	11.35881745869663
18-19	11.623147793677381	29.593732756888286	46.29479708776146	12.488322361672873
20-21	10.550426238623045	29.40230244419536	47.301513549645854	12.745757767535743
22-23	10.807078862232819	29.624131837974097	47.68211487984488	11.886674419948198
24-25	10.746381501512255	29.30744303707392	47.58088272798995	12.365292733423868
26-27	11.230461032344142	28.893052315114296	47.31894087844945	12.557545774092107
28-29	11.038694413683757	29.859412206492298	47.477424462151575	11.624468917672372
30-31	11.092774552821757	29.508836569554237	47.47561381490544	11.922775062718571
32-33	10.89621752974936	29.46234995409851	47.836035338371914	11.805397177780215
34-35	11.083928370892343	29.731681701144396	47.64150810626205	11.542881821701208
36-37	11.211315606595667	29.349023736601687	47.55141710672453	11.888243550078116
38-39	11.055781762557405	29.496238700901618	47.712973536396994	11.735006000143981
40-41	11.283667530787929	30.009867509425465	47.41839683373652	11.288068126050081
42-43	11.36238416529776	29.980908209199402	47.296622089083776	11.36008553641906
44-45	11.243445729509965	29.64355554479407	47.44725563640964	11.665743089286318
46-47	11.463103843185555	29.82582255192769	47.30862007377922	11.402453531107538
48-49	11.360253605173172	29.543013287395198	47.47712664954848	11.619606457883146
50-51	10.990595891572111	29.436891081538256	47.83816106419253	11.734351962697103
52-53	11.296236837843297	29.621267008287017	47.56006062985371	11.522435524015977
54-55	11.276409067588029	29.447048132038788	47.61729206049456	11.659250739878619
56-57	11.08449921581263	29.307528433310083	47.90260415593505	11.705368194942235
58-59	11.23910123737401	29.540269933637315	47.82283299419366	11.397795834795017
60-61	11.245494959437382	29.32617640328124	47.87179161982839	11.556537017452987
62-63	10.883593777744158	29.193483914126677	48.12634272702102	11.796579581108146
64-65	11.26960180231167	29.33241018329985	47.772886107600755	11.625101906787725
66-67	11.228789579695281	29.128117030933836	48.05857595674992	11.584517432620968
68-69	11.069397069809062	29.15398593436887	48.35407681426619	11.422540181555876
70-71	11.21079572851191	29.399233563259543	48.16586447539	11.224106232838544
72-73	11.22376778352244	29.088974738947094	48.33881869719205	11.348438780338412
74-75	11.031931037352601	28.913104071626726	48.496943232985544	11.558021658035123
76-77	11.329877769861769	29.039996351990936	48.31042738169328	11.31969849645401
78-79	11.409704221137895	29.0670177756692	48.352372920838484	11.170905082354421
80-81	11.11352197083596	28.93667949114114	48.697071072505594	11.252727465517308
82-83	11.325867533138153	28.963784404105127	48.52254338522832	11.187804677528405
84-85	11.344482186174085	28.88155940998511	48.356398972345936	11.417559431494869
86-87	11.167235220943637	29.00365027562884	48.372584170631136	11.456530332796385
88-89	11.394538793525706	29.493551493024338	47.934881369099806	11.177028344350145
90-91	11.391198416494401	29.50197982407847	47.91937895579349	11.187442803633639
92-93	11.163882740281947	29.444497604625113	48.04588398644961	11.345735668643329
94-95	11.400160014946614	29.68003344293979	47.73202839416309	11.187778147950507
96-97	11.405934898697998	29.823801069501155	47.72223752423826	11.04802650756259
98-99	11.119691300829219	30.059011559979176	47.83273080937898	10.98856632981262
100	11.553766528754498	30.449285474941686	47.29352736671483	10.70342062958899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	409872.0
1	1052447.0
2	1859197.0
3	1459129.5
4	547784.5
5	136919.5
6	59625.0
7	43456.5
8	42925.0
9	51433.5
10	69029.0
11	98826.5
12	143995.5
13	203702.0
14	277515.0
15	359671.5
16	444059.0
17	525960.5
18	598146.0
19	656544.5
20	709507.0
21	764595.0
22	826961.0
23	894819.5
24	944880.5
25	969925.0
26	979098.0
27	978939.5
28	968580.0
29	943206.5
30	895877.0
31	825297.0
32	747955.0
33	689276.0
34	644993.5
35	589591.0
36	522762.5
37	458160.5
38	402711.0
39	353441.5
40	309224.5
41	271689.5
42	239936.5
43	215353.0
44	200096.5
45	193064.5
46	191717.0
47	193036.5
48	196631.5
49	204962.0
50	216655.5
51	226840.5
52	228897.5
53	217409.0
54	191882.0
55	155003.0
56	113691.5
57	75227.5
58	45602.5
59	26600.5
60	15592.0
61	9752.5
62	6403.5
63	4487.0
64	3384.5
65	2650.5
66	2158.5
67	1797.5
68	1443.0
69	1151.0
70	913.0
71	725.5
72	580.5
73	436.5
74	333.5
75	248.0
76	171.0
77	138.5
78	90.5
79	57.5
80	50.5
81	34.5
82	30.0
83	30.0
84	26.5
85	21.5
86	14.5
87	9.5
88	9.0
89	7.0
90	5.0
91	5.0
92	4.5
93	3.5
94	1.5
95	0.0
96	1.0
97	1.5
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007733063733399759
2	4.7993349348677926E-4
3	1.4073237778935632E-4
4	0.0040451537308171396
5	0.013488657132733691
6	0.007696978508325565
7	0.0
8	0.0
9	0.0
10-11	0.011446233393534315
12-13	0.005308136608413927
14-15	0.0012196806075077547
16-17	2.110985666840345E-4
18-19	7.217045014838787E-5
20-21	8.335686992138797E-4
22-23	1.2990681026709815E-4
24-25	3.3017980942887446E-4
26-27	0.0027244344931016415
28-29	0.0017519376773521152
30-31	0.002468229395074865
32-33	0.0023563651973448636
34-35	0.0027316515381164805
36-37	0.0010356459596293657
38-39	0.0012196806075077547
40-41	4.6910792596452104E-5
42-43	1.8042612537096965E-6
44-45	6.621638801114585E-4
46-47	1.1547272023742058E-4
48-49	4.330227008903272E-4
50-51	0.031764019371559205
52-53	0.03600764184028441
54-55	7.036618889467816E-4
56-57	1.6599203534129207E-4
58-59	5.033888897850053E-4
60-61	0.0040758261721302045
62-63	0.01312419635948433
64-65	0.017564483304863893
66-67	0.018176127869871484
68-69	0.01903676048789101
70-71	0.017079137027615987
72-73	0.014594669281257733
74-75	0.015531080871933068
76-77	0.014103910220248699
78-79	0.015006040847103545
80-81	0.01520992236877274
82-83	0.013636606555537886
84-85	0.013789968762103212
86-87	0.01242594725429868
88-89	0.010571166685485111
90-91	0.008160673650528957
92-93	0.008727211684193803
94-95	0.010416000217666078
96-97	0.005739355048050544
98-99	0.008472810847420735
100	0.009050174448607838
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7712173E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.48906544589044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.07119506775351	35.04922217823535
2	20.125475138133933	20.724838130340377
3	7.278521981097644	11.242928839022658
4	2.527819094029572	5.206201710714405
5	0.9031830090785662	2.3252024532031275
6	0.37499357922726434	1.1584841365572787
7	0.17193885878505338	0.619707980687273
8	0.10511622667151431	0.4329869019611723
9	0.06164358499465229	0.2856573523898065
>10	0.32802671782057435	3.0641015700399503
>50	0.02945165778454307	1.0535401097977077
>100	0.018253019666154023	1.7003640780557236
>500	0.0013639657024766682	0.4897804108633111
>1k	0.00165436355390082	1.7886383881943555
>5k	3.147082386202542E-4	1.1213082874512783
>10k+	0.0010490274620250082	13.737037472486275
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70962	0.25606797417149496	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50408	0.18189840255399675	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47102	0.16996862714446825	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46708	0.16854686927654502	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46074	0.1662590660068411	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45757	0.16511516437198917	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44816	0.16171954469250752	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44571	0.16083545667818977	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43946	0.15858013011105265	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42597	0.15371223324854388	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42531	0.1534740707630542	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41440	0.14953717270745964	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40661	0.14672613367417991	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40392	0.1457554411196841	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39250	0.14163450841621117	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38583	0.13922762390376242	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38267	0.1380873307914179	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37869	0.136651138833465	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37835	0.13652844906821274	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37740	0.1361856394300079	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37212	0.13428033954609045	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36476	0.13162446698062977	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36326	0.13108318860451687	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36005	0.12992485287963523	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35855	0.12938357450352234	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35605	0.1284814438766675	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35600	0.1284634012641304	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35537	0.12823606434616297	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35266	0.1272581547466523	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35087	0.12661222921782425	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34924	0.12602404004911488	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34881	0.12586887358129586	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34699	0.1252121224849455	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34667	0.1250966497647081	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34467	0.12437494526322422	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34246	0.12357746178908453	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34231	0.12352333395147323	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34225	0.12350168281642872	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34196	0.12339703566371357	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33958	0.12253820730694774	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33783	0.12190671586814934	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33068	0.11932662227534449	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32813	0.11840644903595254	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32612	0.11768113601196123	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32099	0.1158299639656551	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31181	0.11251734030384408	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30740	0.11092598187807214	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30716	0.11083937733789408	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30694	0.11075998984273083	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30626	0.11051461031222633	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30291	0.10930575527224083	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30140	0.1087608683736205	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29953	0.10808607466473306	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29882	0.10782986956670629	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29429	0.10619520887084533	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29113	0.10505491575850077	No Hit
TTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28541	0.10299084088425689	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28229	0.10186498186194204	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28160	0.1016159938089301	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27716	0.10001380981563589	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.191346091842022E-4	0.0	0.0	3.608522507419393E-6	0.0
2	0.0018331294337690517	3.608522507419393E-6	0.0	7.217045014838786E-6	0.0
3	0.0029770310686209993	3.608522507419393E-6	0.0	3.247670256677454E-5	0.0
4	0.004983369582746182	3.608522507419393E-6	0.0	3.247670256677454E-5	0.0
5	0.008765101170521705	3.608522507419393E-6	0.0	3.247670256677454E-5	0.0
6	0.01629247912099856	3.608522507419393E-6	0.0	3.247670256677454E-5	0.0
7	0.0247292047433451	3.608522507419393E-6	0.0	3.969374758161332E-5	0.0
8	0.049259940748782136	3.608522507419393E-6	0.0	4.691079259645211E-5	0.0
9	0.14075402892440084	3.608522507419393E-6	0.0	4.691079259645211E-5	0.0
10-11	0.3264792695975159	3.608522507419393E-6	0.0	4.691079259645211E-5	0.0
12-13	0.5280423155556946	3.608522507419393E-6	0.0	5.773636011871029E-5	0.0
14-15	0.7413781661943291	3.608522507419393E-6	0.0	7.036618889467816E-5	0.0
16-17	0.9631399168877879	3.608522507419393E-6	0.0	9.382158519290422E-5	0.0
18-19	1.192277487586412	3.608522507419393E-6	0.0	1.1727698149113027E-4	0.0
20-21	1.4892083706319241	3.608522507419393E-6	0.0	1.3712385528193694E-4	0.0
22-23	1.8284744397344805	3.608522507419393E-6	0.0	1.3712385528193694E-4	0.0
24-25	2.178394671540193	7.217045014838786E-6	0.0	1.3712385528193694E-4	0.0
26-27	2.5027250659845404	7.217045014838786E-6	0.0	1.4073237778935632E-4	0.0
28-29	2.813924047024389	7.217045014838786E-6	0.0	1.4253663904306601E-4	0.0
30-31	3.1469744361079153	7.217045014838786E-6	0.0	1.479494228041951E-4	0.0
32-33	3.5329943270778514	7.217045014838786E-6	0.0	1.515579453116145E-4	0.0
34-35	3.9207787855539156	7.217045014838786E-6	0.0	1.623835128338727E-4	0.0
36-37	4.195519059440052	7.217045014838786E-6	0.0	1.7140481910242114E-4	0.0
38-39	4.390022031112465	7.217045014838786E-6	0.0	1.9125169289322785E-4	0.0
40-41	4.565304207649108	7.217045014838786E-6	0.0	2.1831561169887326E-4	0.0
42-43	4.717692113137429	7.217045014838786E-6	0.0	2.8326901683242234E-4	0.0
44-45	4.84924440966791	7.217045014838786E-6	0.0	3.21158503160326E-4	0.0
46-47	4.968969773680325	7.217045014838786E-6	0.0	3.7167781826419745E-4	0.0
48-49	5.08702258751055	7.217045014838786E-6	0.0	4.3302270089032714E-4	0.0
50-51	5.210435861525546	7.217045014838786E-6	0.0	5.160187185609731E-4	0.0
52-53	5.34070532830464	7.217045014838786E-6	0.0	6.116445650075871E-4	0.0
54-55	5.461641351618294	7.217045014838786E-6	0.0	7.180959789764591E-4	0.0
56-57	5.580488040400152	7.217045014838786E-6	0.0	7.541812040506531E-4	0.0
58-59	5.698856599949776	7.217045014838786E-6	0.0	7.758323390951694E-4	0.0
60-61	5.819839534056026	7.217045014838786E-6	0.0	8.02896257900815E-4	3.608522507419393E-6
62-63	5.940708799703293	7.217045014838786E-6	0.0	8.534155730046864E-4	3.608522507419393E-6
64-65	6.064392712906346	7.217045014838786E-6	0.0	9.147604556308161E-4	3.608522507419393E-6
66-67	6.188262465018532	7.217045014838786E-6	0.0	0.0010176033470922687	3.608522507419393E-6
68-69	6.310618441938855	7.217045014838786E-6	0.0	0.0010735354459572694	3.608522507419393E-6
70-71	6.43447375996101	7.217045014838786E-6	0.0	0.001134880328583399	3.608522507419393E-6
72-73	6.559943891805236	7.217045014838786E-6	0.0	0.0011853996436872708	3.608522507419393E-6
74-75	6.692845775753493	7.217045014838786E-6	0.0	0.001230506175030013	3.608522507419393E-6
76-77	6.830851914788494	7.217045014838786E-6	0.0	0.0013477831565211432	3.608522507419393E-6
78-79	6.96867582343687	1.8042612537096967E-5	0.0	0.001587749903264533	3.608522507419393E-6
80-81	7.1095633676940455	2.8868180059355144E-5	0.0	0.001836737956276471	3.608522507419393E-6
82-83	7.2566810260602805	2.8868180059355144E-5	0.0	0.001919733973947117	3.608522507419393E-6
84-85	7.4080477196790016	3.067244131306484E-5	0.0	0.0019395808477379237	3.608522507419393E-6
86-87	7.563888981206922	3.247670256677454E-5	0.0	0.001966644766543569	3.608522507419393E-6
88	7.686387494766289	3.247670256677454E-5	0.0	0.001973861811558408	3.608522507419393E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAGGC	1480	0.0	62.566326	1
GCTTTCT	5825	0.0	62.134235	1
GCCTTTT	6830	0.0	54.643177	1
GCTTTTC	6300	0.0	52.525265	1
GATTTCT	7165	0.0	47.824177	1
GCATATC	1905	0.0	46.390057	6
GCCCTAC	8650	0.0	44.55908	7
AGCCGCG	150	0.0	43.86713	4
GCCGCGT	410	0.0	43.570007	5
GGCATAT	2060	0.0	42.9021	5
GCCAATG	720	0.0	41.778744	2
GACTTTT	7525	0.0	41.16376	1
TGCTCTA	3910	0.0	41.11605	6
AGGCATA	2150	0.0	41.098106	4
CCGCGTG	465	0.0	39.42518	6
GGCTCTA	1595	0.0	38.31287	6
TGCCCTA	4670	0.0	38.1491	6
TTTGACG	2630	0.0	37.88633	4
CTAGCTT	1210	0.0	37.681007	1
TTAGGCA	2460	0.0	37.44802	2
>>END_MODULE