FastQCFastQC Report
Thu 26 May 2016
SRR1248444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11526952
Sequences flagged as poor quality0
Sequence length100
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6487445.62806195427898No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1628261.4125676935238387No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1266541.0987640097746567No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1253761.087676950506951No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA707150.6134752708261473No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460330.39935101664342837No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426620.3701065121117881No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415750.3606764390100696No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA353320.306516414746934No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA322230.27954484411837577No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270870.23498839936177401No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA222900.1933728881667938No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA202990.17610032556741798No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184620.160163762285121No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183150.15888849020972762No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171990.14920683282102676No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160670.13938637030847356No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160210.1389873055773981No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129420.11227599455606305No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126560.10979485296720244No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123580.10720960753545256No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121540.10543984220633519No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATCC957.2759576E-1249.4840431
CTTGGCC1450.048.6266483
TTGGCCG1450.042.1407134
CCCGACG17350.041.4460038
AGGCACC1156.184564E-1140.8745733
GGCTACC1500.040.7383233
CTTGCGC1051.1405064E-940.2913482
GGACCCT2050.038.9837721
TGCCGCA1450.038.9046971
GGACCTC853.9533006E-738.7139851
CAGTCGC853.9559927E-738.7106253
TGGCTCC1101.7953425E-938.4625931
TTTGCCG2200.038.457084
CCGTTGT5450.037.9528961
CTAGGAC757.442295E-637.605262
CATTGCC3500.037.6046033
TCGCACG500.001702112837.602484
CCGTTTG4150.037.381321
GGACCCA2050.036.690611
CCGTGTT5300.036.3661041
Produced by FastQC (version 0.11.5)