##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11526952 Sequences flagged as poor quality 0 Sequence length 100 %GC 24 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83523875175328 34.0 31.0 34.0 31.0 34.0 2 33.02389998674411 34.0 33.0 34.0 31.0 34.0 3 33.10304050888735 34.0 33.0 34.0 31.0 34.0 4 36.43282239745598 37.0 37.0 37.0 35.0 37.0 5 36.343475100789874 37.0 37.0 37.0 35.0 37.0 6 36.26797561055169 37.0 37.0 37.0 35.0 37.0 7 36.30788980469425 37.0 37.0 37.0 35.0 37.0 8 36.10817855405315 37.0 36.0 37.0 35.0 37.0 9 37.912512344980705 39.0 39.0 39.0 35.0 39.0 10-11 38.09868515111367 39.0 39.0 39.0 36.0 39.0 12-13 38.155908257447415 39.0 39.0 39.0 37.0 39.0 14-15 39.74678700839563 41.0 40.0 41.0 37.0 41.0 16-17 39.7458600070513 41.0 40.0 41.0 37.0 41.0 18-19 39.62982386844328 41.0 39.5 41.0 37.0 41.0 20-21 39.33586788597714 41.0 39.0 41.0 35.5 41.0 22-23 38.94461120337796 40.5 38.5 41.0 35.0 41.0 24-25 38.6162875927652 40.0 37.0 41.0 35.0 41.0 26-27 38.471689480445484 40.0 36.0 41.0 35.0 41.0 28-29 38.37468226639618 40.0 36.0 41.0 35.0 41.0 30-31 38.30003785909753 40.0 35.0 41.0 35.0 41.0 32-33 38.23651742455421 40.0 35.0 41.0 35.0 41.0 34-35 38.17284269944041 40.0 35.0 41.0 35.0 41.0 36-37 38.10969200704575 40.0 35.0 41.0 35.0 41.0 38-39 38.027862699523695 40.0 35.0 41.0 35.0 41.0 40-41 37.94288416400103 40.0 35.0 41.0 34.5 41.0 42-43 37.84015813547241 40.0 35.0 41.0 34.0 41.0 44-45 37.75908280003248 40.0 35.0 41.0 34.0 41.0 46-47 37.682724626596865 40.0 35.0 41.0 34.0 41.0 48-49 37.617929917639984 40.0 35.0 41.0 34.0 41.0 50-51 37.46655685735483 39.5 35.0 41.0 33.5 41.0 52-53 37.25519634331782 39.0 35.0 41.0 33.0 41.0 54-55 37.120007960473856 39.0 35.0 41.0 33.0 41.0 56-57 36.947052048104304 39.0 35.0 41.0 33.0 41.0 58-59 36.74784353227115 38.0 35.0 41.0 33.0 41.0 60-61 36.49219516139219 37.0 35.0 40.5 32.5 41.0 62-63 36.21331744940033 37.0 35.0 40.0 32.0 41.0 64-65 35.962837400554804 36.0 35.0 40.0 32.0 41.0 66-67 35.70634123400531 36.0 35.0 39.0 31.5 41.0 68-69 35.46419495804268 35.0 35.0 39.0 31.0 41.0 70-71 35.277168630527825 35.0 35.0 39.0 31.0 41.0 72-73 35.06889002400635 35.0 35.0 37.5 31.0 40.0 74-75 34.812709508983815 35.0 35.0 37.0 31.0 39.5 76-77 34.5664022024209 35.0 35.0 37.0 31.0 39.0 78-79 34.325594528371425 35.0 34.5 36.0 31.0 39.0 80-81 34.108223969354604 35.0 34.0 36.0 31.0 38.5 82-83 33.91477079109899 35.0 34.0 36.0 31.0 37.0 84-85 33.73338901732218 35.0 34.0 35.0 30.0 37.0 86-87 33.57749750324284 35.0 34.0 35.0 30.0 36.5 88-89 33.43499183478859 35.0 34.0 35.0 30.0 36.0 90-91 33.316567293765075 35.0 34.0 35.0 30.0 36.0 92-93 33.19049498080672 35.0 34.0 35.0 29.5 36.0 94-95 33.071431155434674 35.0 34.0 35.0 29.0 36.0 96-97 32.97869983322565 35.0 34.0 35.0 29.0 35.5 98-99 32.87616852225983 35.0 34.0 35.0 29.0 35.0 100 32.30749559814251 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19367194781721508 1101 2 0.16413007584207406 1101 3 0.18134468653138924 1101 4 0.07224216717249732 1101 5 0.1069063894225053 1101 6 0.012955647524393044 1101 7 0.06224764093735757 1101 8 0.0012162811586406974 1101 9 -0.043549520648106466 1101 10-11 0.046611537768662004 1101 12-13 0.15532021763252857 1101 14-15 0.18994480865767116 1101 16-17 0.22435938143734546 1101 18-19 0.2294029828937525 1101 20-21 0.25620075593737823 1101 22-23 0.236898727267139 1101 24-25 0.217544659883103 1101 26-27 0.2318470330229303 1101 28-29 0.26517993762876557 1101 30-31 0.28424897896305623 1101 32-33 0.2809699284226568 1101 34-35 0.2655164124412579 1101 36-37 0.2539578377623073 1101 38-39 0.26382030701860515 1101 40-41 0.24956205416727784 1101 42-43 0.28326651443983764 1101 44-45 0.2492935366472011 1101 46-47 0.2661179104104505 1101 48-49 0.2529486874402238 1101 50-51 0.22391183845769547 1101 52-53 0.18796748054456458 1101 54-55 0.22627381655171774 1101 56-57 0.24220409393886655 1101 58-59 0.20953411167903369 1101 60-61 0.20350533751124544 1101 62-63 0.18029442220718295 1101 64-65 0.1586538396930166 1101 66-67 0.15091947918005388 1101 68-69 0.17800196240759192 1101 70-71 0.19839266512160236 1101 72-73 0.21585203769107864 1101 74-75 0.19004183948658238 1101 76-77 0.20974398714438536 1101 78-79 0.20265196513931016 1101 80-81 0.1773951943143075 1101 82-83 0.15342294477891016 1101 84-85 0.169073380479702 1101 86-87 0.16680457506572566 1101 88-89 0.21570049756158483 1101 90-91 0.18525315396320963 1101 92-93 0.2184236229265295 1101 94-95 0.22364135186032286 1101 96-97 0.2241292416804157 1101 98-99 0.2233961588040998 1101 100 0.19710974127562508 1102 1 0.14230177737344718 1102 2 0.14632096968219344 1102 3 0.1629515917745863 1102 4 0.0812453008689289 1102 5 0.08663817462249312 1102 6 -0.030165648228837938 1102 7 -0.011563122383854818 1102 8 -0.08306951657854 1102 9 -0.05416441595612298 1102 10-11 0.01813464147781474 1102 12-13 0.09466752069172202 1102 14-15 0.14522061954331633 1102 16-17 0.15944571900495674 1102 18-19 0.19197600506935686 1102 20-21 0.1919526700062093 1102 22-23 0.1693166797872152 1102 24-25 0.16608713717223367 1102 26-27 0.1531930463524276 1102 28-29 0.15466628913382152 1102 30-31 0.15569011429135315 1102 32-33 0.17010873033117235 1102 34-35 0.17085348863400185 1102 36-37 0.17058834485288088 1102 38-39 0.19366648120672636 1102 40-41 0.17114779633199362 1102 42-43 0.1490930602717313 1102 44-45 0.17097472693347981 1102 46-47 0.1868382972767222 1102 48-49 0.16805036536470652 1102 50-51 0.15795087855597956 1102 52-53 0.16388042544967618 1102 54-55 0.18555630299860582 1102 56-57 0.13658729310129303 1102 58-59 0.1417438748675579 1102 60-61 0.16068298762053956 1102 62-63 0.1433597467127683 1102 64-65 0.15685761847171165 1102 66-67 0.15013827213564213 1102 68-69 0.1517250788069262 1102 70-71 0.1510360788922469 1102 72-73 0.10586062902326887 1102 74-75 0.1209368559360513 1102 76-77 0.1451512266462487 1102 78-79 0.12191090723941755 1102 80-81 0.1227611501077348 1102 82-83 0.14034055890370212 1102 84-85 0.12603803523785473 1102 86-87 0.11557818470856773 1102 88-89 0.14127785356537004 1102 90-91 0.13484391567211418 1102 92-93 0.15550860668418665 1102 94-95 0.18209120971370396 1102 96-97 0.16547886696250202 1102 98-99 0.15429124677171302 1102 100 0.08166643259660589 1103 1 0.17115682897279072 1103 2 0.15116463758732124 1103 3 0.15258801818216483 1103 4 0.09522115405916765 1103 5 0.10825090892365097 1103 6 0.054230415843811386 1103 7 0.040303352777726786 1103 8 -0.04955459943045781 1103 9 -0.11389602090874007 1103 10-11 -0.009367819100688735 1103 12-13 0.07743109736060916 1103 14-15 0.12971183001080533 1103 16-17 0.16416977394418097 1103 18-19 0.1786502964853085 1103 20-21 0.16468525894155306 1103 22-23 0.10186882401970365 1103 24-25 0.10409040830442251 1103 26-27 0.10990595282680715 1103 28-29 0.1160536264423655 1103 30-31 0.09924541022957811 1103 32-33 0.14082692697051158 1103 34-35 0.14461633837969856 1103 36-37 0.15186139103108331 1103 38-39 0.14012183426839186 1103 40-41 0.13582386851395967 1103 42-43 0.08881760177142439 1103 44-45 0.10507423513395509 1103 46-47 0.12099991665012766 1103 48-49 0.10114861010659837 1103 50-51 0.11859856580313988 1103 52-53 0.09811629891460427 1103 54-55 0.11819907616713721 1103 56-57 0.14311500996464588 1103 58-59 0.1277606013749022 1103 60-61 0.11432354696338365 1103 62-63 0.09708095022205754 1103 64-65 0.07659210948234119 1103 66-67 0.047284350109706 1103 68-69 0.04341401726041738 1103 70-71 0.06909353270859242 1103 72-73 0.06410904296804176 1103 74-75 0.0804107010752162 1103 76-77 0.032241698001882924 1103 78-79 0.06737331193304641 1103 80-81 0.06747731039583016 1103 82-83 0.0639581794318218 1103 84-85 0.04225875993557793 1103 86-87 0.06458774041504967 1103 88-89 0.062334836967394835 1103 90-91 0.07818020389332503 1103 92-93 0.0644057260250932 1103 94-95 0.0863135696133952 1103 96-97 0.07660377113247563 1103 98-99 0.11162949246070752 1103 100 0.0686012738774906 1104 1 -0.22489505047929725 1104 2 -0.2200606205376019 1104 3 -0.17675169289886838 1104 4 -0.2394795415347062 1104 5 -0.1247854785647462 1104 6 -0.04042410506497873 1104 7 -0.010633283904581958 1104 8 -0.09421879495809549 1104 9 -0.0035733940840643186 1104 10-11 0.07457671811291533 1104 12-13 0.08916288784107707 1104 14-15 0.13628213144983903 1104 16-17 0.17367126254058718 1104 18-19 0.1483909079854655 1104 20-21 0.12597404107019372 1104 22-23 0.13464190927430053 1104 24-25 0.11965813029841854 1104 26-27 0.14097824334260167 1104 28-29 0.1280095901605094 1104 30-31 0.16257892311342914 1104 32-33 0.1382067034469756 1104 34-35 0.13337834726993236 1104 36-37 0.13546349821654502 1104 38-39 0.13879634140202768 1104 40-41 0.13705550410291778 1104 42-43 0.17623928235070707 1104 44-45 0.18434123326321128 1104 46-47 0.17383120547297182 1104 48-49 0.1530403520603727 1104 50-51 0.17576588896805845 1104 52-53 0.11097047617273148 1104 54-55 0.1649297796244369 1104 56-57 0.1505044021553701 1104 58-59 0.15503617864794705 1104 60-61 0.15328334884908656 1104 62-63 0.12789228133789265 1104 64-65 0.15655755034714502 1104 66-67 0.15212691088349573 1104 68-69 0.12098323888499607 1104 70-71 0.135045742269142 1104 72-73 0.12438348275688327 1104 74-75 0.09580650887610176 1104 76-77 0.09362906312405528 1104 78-79 0.10220730250720322 1104 80-81 0.07605679626077233 1104 82-83 0.06515401129681209 1104 84-85 0.09918135166280706 1104 86-87 0.10468869344197174 1104 88-89 0.05572793083558025 1104 90-91 0.06362822092852838 1104 92-93 0.10018171852120616 1104 94-95 0.07573213724401029 1104 96-97 0.11351950264509014 1104 98-99 0.11257431028872844 1104 100 0.09332955125025677 1105 1 0.09121926616688825 1105 2 0.0871625344424487 1105 3 0.10735901811784743 1105 4 0.058056587996112796 1105 5 0.09485832567817454 1105 6 -0.023430570364801895 1105 7 0.001571611901155734 1105 8 -0.08040783198239865 1105 9 -0.06835665554822157 1105 10-11 0.022174576561717174 1105 12-13 0.038227325713705795 1105 14-15 0.09129647160571608 1105 16-17 0.11684959280272977 1105 18-19 0.10887389423492522 1105 20-21 0.09178614228546422 1105 22-23 0.0801615426764144 1105 24-25 0.06886963981651917 1105 26-27 0.07821790322235245 1105 28-29 0.07530974013636182 1105 30-31 0.07587048639573624 1105 32-33 0.07131180518614855 1105 34-35 0.05495423012613543 1105 36-37 0.05863738714531763 1105 38-39 0.05397309721039534 1105 40-41 0.05692511608948081 1105 42-43 0.09404816392266468 1105 44-45 0.07570153208730801 1105 46-47 0.10878126918324682 1105 48-49 0.054229834022549994 1105 50-51 0.07228507320456146 1105 52-53 -0.11990587963956045 1105 54-55 -0.09042650407905484 1105 56-57 -0.014531876016725676 1105 58-59 -0.013462687900229753 1105 60-61 0.010766432415387328 1105 62-63 -0.04364245994892002 1105 64-65 -0.02220263962531277 1105 66-67 -0.01597210709343244 1105 68-69 0.04046575255853213 1105 70-71 0.013010717605574484 1105 72-73 -0.01092258882853514 1105 74-75 0.0038936675748004745 1105 76-77 -0.016147903506450234 1105 78-79 0.00650943682233418 1105 80-81 -0.020909765471230912 1105 82-83 -0.0022119129743316535 1105 84-85 -0.010417608269492007 1105 86-87 -5.694325977145809E-4 1105 88-89 0.041827763377717986 1105 90-91 -0.008114638646851802 1105 92-93 -0.011953201911261147 1105 94-95 -0.010616582862752466 1105 96-97 -7.695094339865705E-4 1105 98-99 -0.052416898303711434 1105 100 -0.10939083879706146 1106 1 0.08643178124275863 1106 2 0.08417465124262691 1106 3 0.0837618509635476 1106 4 0.04920450188438252 1106 5 0.05152778200300645 1106 6 0.0285485454269363 1106 7 0.04495637334606073 1106 8 -0.042508519877053175 1106 9 -0.028375358742430024 1106 10-11 0.0413472087780562 1106 12-13 0.07136367563686719 1106 14-15 0.08717285459917434 1106 16-17 0.08083610021920151 1106 18-19 0.08514677200191301 1106 20-21 0.071051185562375 1106 22-23 0.060655294298150864 1106 24-25 0.0516538389948451 1106 26-27 0.07598138670690702 1106 28-29 0.09100619222605388 1106 30-31 0.05762371382949283 1106 32-33 0.051830124234790276 1106 34-35 0.03958202152484347 1106 36-37 0.05172298864020064 1106 38-39 0.051578636064981254 1106 40-41 0.041700541060166074 1106 42-43 0.0572138679272598 1106 44-45 0.09041749528299903 1106 46-47 0.07777712428688233 1106 48-49 0.06284399126082718 1106 50-51 0.0980112653049332 1106 52-53 0.009059730364228358 1106 54-55 0.03110649390198006 1106 56-57 0.060872185449817096 1106 58-59 0.03852003620323785 1106 60-61 0.07499066501629414 1106 62-63 0.029686369834024617 1106 64-65 0.04661871245847493 1106 66-67 0.03646546537116535 1106 68-69 0.009688888478436297 1106 70-71 0.002940880277279234 1106 72-73 0.005636169705489635 1106 74-75 0.04398173540474204 1106 76-77 0.041184943351481706 1106 78-79 0.011952491291815193 1106 80-81 0.04643674411573784 1106 82-83 0.04835085667667727 1106 84-85 0.010436337094681392 1106 86-87 0.021843446800716038 1106 88-89 0.024796282492545174 1106 90-91 0.03367858735074236 1106 92-93 0.05663405489755746 1106 94-95 0.0521399549754733 1106 96-97 0.04406395565919752 1106 98-99 0.05677179711852176 1106 100 0.06999796547627568 1107 1 0.055569468027172775 1107 2 0.0548319430187334 1107 3 0.059332075567510856 1107 4 0.025864138695673944 1107 5 0.021666918012719805 1107 6 0.00499792375634911 1107 7 0.005317482274570295 1107 8 -0.09563296693146839 1107 9 -0.08971382936496752 1107 10-11 -0.005194252721892667 1107 12-13 0.013577394575925439 1107 14-15 0.03032553443100028 1107 16-17 0.04339707659290326 1107 18-19 0.059205674947129694 1107 20-21 0.030827680549151637 1107 22-23 0.05183713511318899 1107 24-25 0.06106613401681216 1107 26-27 0.06958380068633829 1107 28-29 0.06772637361457612 1107 30-31 0.07401395796767218 1107 32-33 0.05147537815170011 1107 34-35 0.04322241211584554 1107 36-37 0.06756863227467846 1107 38-39 0.08047154391755384 1107 40-41 0.047529777323624955 1107 42-43 0.06346799191916119 1107 44-45 0.027601392970595384 1107 46-47 0.0702592550722585 1107 48-49 0.0940591604990928 1107 50-51 0.041328817111107696 1107 52-53 -0.012400674212507568 1107 54-55 0.049545522360809 1107 56-57 0.08689795577920023 1107 58-59 0.07974138572426881 1107 60-61 0.07866355712910433 1107 62-63 0.04966946668011474 1107 64-65 0.03431339360295027 1107 66-67 0.03419773473412846 1107 68-69 0.07914917063081361 1107 70-71 0.0985861613996093 1107 72-73 0.0635322711516153 1107 74-75 0.07159724163219039 1107 76-77 0.03884440422248758 1107 78-79 0.04551957581814037 1107 80-81 0.03853775030442819 1107 82-83 0.06495097448328835 1107 84-85 0.04987326912571177 1107 86-87 0.058856363824375535 1107 88-89 0.034414517136745815 1107 90-91 0.0021339981229715477 1107 92-93 0.012386697178747852 1107 94-95 0.00617427639635082 1107 96-97 0.0013671759391229443 1107 98-99 0.030696036839032104 1107 100 0.035779019517867994 1108 1 0.05646126576768751 1108 2 0.030505332532968055 1108 3 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2214 20-21 -0.18657078942056415 2214 22-23 -0.16798329532694822 2214 24-25 -0.16417133262505246 2214 26-27 -0.19205966692446452 2214 28-29 -0.18319290779275121 2214 30-31 -0.1866298763395875 2214 32-33 -0.17332613756376958 2214 34-35 -0.160420946823713 2214 36-37 -0.15844558959033606 2214 38-39 -0.15953932734302612 2214 40-41 -0.15533985760308866 2214 42-43 -0.1663303470934423 2214 44-45 -0.17539955073149827 2214 46-47 -0.18605460841212818 2214 48-49 -0.14217779602234515 2214 50-51 -0.16024237447585676 2214 52-53 -0.12596581468731927 2214 54-55 -0.14325038299536885 2214 56-57 -0.1098007197142934 2214 58-59 -0.12533388496432707 2214 60-61 -0.11921575408834428 2214 62-63 -0.10115944246066988 2214 64-65 -0.08672007672324611 2214 66-67 -0.07472889411734229 2214 68-69 -0.08448712054135399 2214 70-71 -0.08182530402233112 2214 72-73 -0.07756535795374475 2214 74-75 -0.07575333714555654 2214 76-77 -0.06381257433935161 2214 78-79 -0.08154976511703182 2214 80-81 -0.09870030272078623 2214 82-83 -0.09578973581561456 2214 84-85 -0.08449867940880296 2214 86-87 -0.10206512410545798 2214 88-89 -0.1312044393187719 2214 90-91 -0.10950314642176551 2214 92-93 -0.06117002127546556 2214 94-95 -0.03854935596996256 2214 96-97 -0.05300241023724084 2214 98-99 -0.018671588979991327 2214 100 -0.041282733738029265 2215 1 -0.21764821990780803 2215 2 -0.20509286415673245 2215 3 -0.21625441501253562 2215 4 -0.13423628878696547 2215 5 -0.17004548759270932 2215 6 -0.10640060897472381 2215 7 -0.09766018047955782 2215 8 0.07527034235988594 2215 9 0.08713864473453725 2215 10-11 -0.058457365731733546 2215 12-13 -0.1539276832937091 2215 14-15 -0.2685673151214729 2215 16-17 -0.3092273816555675 2215 18-19 -0.3124367434908564 2215 20-21 -0.3001281830115161 2215 22-23 -0.29837982107051886 2215 24-25 -0.3109210415039527 2215 26-27 -0.3256099788897302 2215 28-29 -0.3223418648825387 2215 30-31 -0.31257722442940405 2215 32-33 -0.31557477640385656 2215 34-35 -0.3698022764190583 2215 36-37 -0.3406108942930075 2215 38-39 -0.3471085788122039 2215 40-41 -0.3152026814951938 2215 42-43 -0.3665014467038503 2215 44-45 -0.3810631205896726 2215 46-47 -0.36413158888984754 2215 48-49 -0.3760368415024615 2215 50-51 -0.3477110687141689 2215 52-53 -0.29041218046333483 2215 54-55 -0.28883547793243025 2215 56-57 -0.31228436597076836 2215 58-59 -0.3552150051277607 2215 60-61 -0.28387380388523553 2215 62-63 -0.27322577989585284 2215 64-65 -0.2788922016040729 2215 66-67 -0.26640191122739054 2215 68-69 -0.2659035181516032 2215 70-71 -0.21022639728173687 2215 72-73 -0.2501545177784976 2215 74-75 -0.27753114036350013 2215 76-77 -0.2679600486992655 2215 78-79 -0.23435486965158958 2215 80-81 -0.2405663117222474 2215 82-83 -0.25631991876441873 2215 84-85 -0.2695254842068522 2215 86-87 -0.24050581539549398 2215 88-89 -0.2681780749576461 2215 90-91 -0.21590286384775226 2215 92-93 -0.23143004486009744 2215 94-95 -0.19437204857116086 2215 96-97 -0.19879653995845104 2215 98-99 -0.18914966318060777 2215 100 -0.14450803144377034 2216 1 -0.24044419525515082 2216 2 -0.1981903763687214 2216 3 -0.2300728291041807 2216 4 -0.11716512502723475 2216 5 -0.16146204156424915 2216 6 -0.07249794218992633 2216 7 -0.09507171955395677 2216 8 0.06416577341393292 2216 9 0.022551853345028405 2216 10-11 -0.12755671358442555 2216 12-13 -0.21840242285417588 2216 14-15 -0.32687994302615664 2216 16-17 -0.3557758527974286 2216 18-19 -0.3820138445274992 2216 20-21 -0.3092717683037236 2216 22-23 -0.22975565975927736 2216 24-25 -0.19000080819339615 2216 26-27 -0.20111632675083513 2216 28-29 -0.1640268845497772 2216 30-31 -0.20095319730679506 2216 32-33 -0.20968862008738398 2216 34-35 -0.21810603230927228 2216 36-37 -0.22137827559208034 2216 38-39 -0.2003467324206838 2216 40-41 -0.22095667144510855 2216 42-43 -0.23103039626944621 2216 44-45 -0.23081148546545194 2216 46-47 -0.2557594074111691 2216 48-49 -0.24254228070137174 2216 50-51 -0.2877646628037098 2216 52-53 -0.23793396135319256 2216 54-55 -0.24289969933690259 2216 56-57 -0.2371038854088212 2216 58-59 -0.24966698725575043 2216 60-61 -0.21048151258570158 2216 62-63 -0.22681080757353556 2216 64-65 -0.26189804301512964 2216 66-67 -0.2415455960408579 2216 68-69 -0.2312653333817991 2216 70-71 -0.1963529772506547 2216 72-73 -0.19982728461674526 2216 74-75 -0.1687287278924927 2216 76-77 -0.19155116045952525 2216 78-79 -0.18705464739528566 2216 80-81 -0.179863259210002 2216 82-83 -0.18279515255096612 2216 84-85 -0.17020568377450473 2216 86-87 -0.21341399389766025 2216 88-89 -0.18092659021588986 2216 90-91 -0.207338252966089 2216 92-93 -0.18333296264916044 2216 94-95 -0.2123826526173076 2216 96-97 -0.22162271167790948 2216 98-99 -0.2234560376750494 2216 100 -0.17375442354231296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 98.0 8 311.0 9 633.0 10 1042.0 11 1728.0 12 2624.0 13 3794.0 14 4944.0 15 6136.0 16 7743.0 17 9770.0 18 12354.0 19 15708.0 20 20239.0 21 26039.0 22 32069.0 23 38305.0 24 45773.0 25 54993.0 26 66135.0 27 79574.0 28 95888.0 29 119103.0 30 139712.0 31 173936.0 32 223745.0 33 314013.0 34 450558.0 35 2053799.0 36 1066797.0 37 2332564.0 38 2982275.0 39 1132043.0 40 12496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.418805313439098 23.09521613663833 34.028158378014126 24.457820171908445 2 13.850880185222522 25.79749163955037 37.99430063194832 22.357327543278796 3 11.521327089870196 21.83430184742936 42.39897749063585 24.245393572064597 4 11.7547799009829 20.528783513876814 45.896315046014855 21.820121539125427 5 10.043998434762841 20.42133859012391 48.20887933704744 21.325783638065804 6 9.081225918214916 16.845508801035425 45.89948869898344 28.173776581766212 7 11.40929536272902 18.256899135174677 43.68749865532536 26.64630684677094 8 4.4952646631997775 18.741398419981277 47.45415787278372 29.309179044035233 9 7.67090901393534 16.405828704760808 47.80009494270471 28.123167338599135 10-11 11.744225155563303 31.288946137227313 45.14908468863718 11.817744018572201 12-13 10.47233358697037 32.131483322920204 45.333683662170046 12.062499427939384 14-15 10.574533291928468 31.032516281260612 45.57462937215455 12.818321054656376 16-17 11.078984404126773 31.059278372315607 45.972367745004036 11.889369478553586 18-19 11.169907168374065 30.316364410360443 45.699959776851536 12.813768644413964 20-21 10.389308497689834 30.22520676464408 46.411971710630304 12.973513027035777 22-23 10.43325488380724 30.261718532608533 46.958480627715346 12.346545955868878 24-25 10.357231841450686 29.973196637573015 46.938646707808644 12.730924813167654 26-27 10.998203728661958 29.70074579312038 46.47110111302889 12.829949365188773 28-29 10.661233110723934 30.41698509217719 46.82587060187822 12.095911195220655 30-31 10.673150456397085 30.14026570495706 46.89503365882415 12.29155017982171 32-33 10.713532185067173 30.17042067156705 47.00955051188925 12.10649663147652 34-35 10.703263296780861 30.241271298483696 47.012240263809026 12.043225140926415 36-37 10.799506525703508 29.97883678716325 46.958292954752615 12.263363732380634 38-39 10.834301256664292 30.20231806233208 46.933871775884874 12.02950890511876 40-41 10.880906509131995 30.53974670129394 46.8062957536707 11.773051035903357 42-43 10.958729593044197 30.607301912942813 46.72624211500143 11.707726379011554 44-45 11.034689262429273 30.362124845393996 46.660844334213216 11.94234155796351 46-47 11.05859550099276 30.39773858515653 46.69666536912692 11.847000544723784 48-49 10.987198125845739 30.21387959613794 46.85940442300822 11.9395178550081 50-51 10.84722005208531 30.24129429901502 47.000746883865396 11.910738765034276 52-53 10.90623484805816 30.269781910971254 46.9754782296726 11.848505011297974 54-55 10.860450035243725 30.18554465108713 46.95403567749282 11.999969636176328 56-57 10.883020661263494 30.12165508379375 46.99492918020778 12.000395074734971 58-59 10.83975294688587 30.222399507795405 47.0824628343718 11.85538471094692 60-61 10.830369728757304 30.147089698663187 47.121082853916505 11.901457718663005 62-63 10.706281980924782 30.113401458080723 47.13074788999104 12.049568671003454 64-65 10.837612174236577 30.057858062106767 46.98781198459548 12.11671777906118 66-67 10.788179977174476 29.942627288572133 47.242320637282134 12.026872096971259 68-69 10.867356640623695 30.029498727280874 47.26519730237603 11.837947329719396 70-71 10.834222597696629 30.18720950860156 47.237332521878244 11.741235371823567 72-73 10.880735483656572 29.98550271254196 47.39684603197004 11.736915771831429 74-75 10.844246546537049 29.844180994156726 47.415867467750594 11.89570499155563 76-77 10.970787913683447 29.809285625412898 47.36504427043882 11.854882190464837 78-79 11.07162320722029 29.928368202898888 47.33835167475345 11.661656915127379 80-81 11.048126846336316 29.834269962948113 47.479846100571535 11.637757090144031 82-83 11.081007447923536 29.684122665148855 47.54653650731951 11.688333379608098 84-85 11.099994533779075 29.61006756422593 47.46729160900382 11.822646292991177 86-87 11.129915782432093 29.774066299047202 47.28026233991483 11.815755578605883 88-89 11.174629635863694 30.096109697263955 47.04937251446422 11.679888152408134 90-91 11.167939292579373 30.177169881081173 47.05769487630609 11.597195950033367 92-93 11.164284603433268 30.1420475064957 47.011072239710614 11.682595650360417 94-95 11.225276281519944 30.19574475247639 46.88651191075318 11.692467055250491 96-97 11.214894486082727 30.405039730174217 46.893864545040316 11.486201238702746 98-99 11.15103268217127 30.672082280544327 46.82264103143064 11.354244005853756 100 11.228530315998524 30.867793207352694 46.5981019179071 11.305574558741679 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 149956.0 1 386122.0 2 698249.0 3 567082.5 4 223763.0 5 59857.5 6 26102.0 7 18818.5 8 18457.0 9 21812.0 10 28311.5 11 39284.0 12 56408.5 13 80079.0 14 110475.0 15 145572.0 16 182235.5 17 218041.5 18 252053.0 19 281088.5 20 304203.0 21 327487.0 22 353275.0 23 378992.5 24 401064.0 25 414335.0 26 419380.5 27 421880.0 28 424071.5 29 419800.0 30 405037.0 31 381849.0 32 351404.0 33 319726.5 34 289515.0 35 259771.5 36 232571.0 37 207422.5 38 183092.0 39 159761.5 40 137850.5 41 117748.0 42 100081.5 43 86262.0 44 76375.5 45 70649.0 46 68286.0 47 67072.0 48 67156.5 49 69927.0 50 74072.0 51 77950.5 52 79117.5 53 75231.5 54 66165.0 55 53110.5 56 38717.0 57 25039.5 58 14546.0 59 7945.5 60 4138.5 61 2194.5 62 1179.0 63 683.5 64 470.5 65 330.5 66 216.0 67 174.5 68 147.0 69 129.0 70 108.0 71 78.5 72 68.0 73 54.5 74 45.0 75 41.0 76 33.0 77 24.0 78 18.0 79 12.0 80 9.5 81 11.5 82 10.5 83 6.5 84 9.0 85 8.5 86 3.0 87 3.0 88 5.0 89 4.0 90 2.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008094073784639687 2 5.20519214446282E-4 3 1.3880512385234189E-4 4 0.003973296670273286 5 0.013377343811269449 6 0.007504152008267234 7 0.0 8 0.0 9 0.0 10-11 0.011611916142272477 12-13 0.005539191973732519 14-15 0.001097428010457578 16-17 2.1254534589889852E-4 18-19 6.506490180578525E-5 20-21 6.766749787801667E-4 22-23 6.506490180578525E-5 24-25 3.470128096308547E-4 26-27 0.0025765701115090965 28-29 0.001604934244542703 30-31 0.0022295573018782417 32-33 0.002082076857785128 34-35 0.002433427327536369 36-37 9.586228866052362E-4 38-39 0.001097428010457578 40-41 6.940256192617094E-5 42-43 0.0 44-45 6.159477370947671E-4 46-47 7.807788216694231E-5 48-49 3.9906473107548295E-4 50-51 0.03245871068084607 52-53 0.035473384464514125 54-55 6.679996585393954E-4 56-57 1.6483108457465598E-4 58-59 3.2966216914931195E-4 60-61 0.003886543467865573 62-63 0.01324721400765788 64-65 0.01755884816732125 66-67 0.01852180871404687 68-69 0.01923752263391051 70-71 0.01714677045588461 72-73 0.01468297950750554 74-75 0.015767394537601963 76-77 0.014348979678235842 78-79 0.015294589584479924 80-81 0.015294589584479924 82-83 0.013811109823308017 84-85 0.01407136943053116 86-87 0.01255318838839617 88-89 0.011000306065298095 90-91 0.008506151496076326 92-93 0.009148125193893407 94-95 0.01087451392180691 96-97 0.006072724168539958 98-99 0.00884448898546641 100 0.009603579506533904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1526952E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.42463310327673 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93634224124084 56.593770796467325 2 12.728514167096552 17.1643079533269 3 2.2867800059925463 4.625559086758692 4 0.46872435320991734 1.2641427016699747 5 0.16269969768677786 0.548498371127252 6 0.09099625718932663 0.36812335528570544 7 0.0610984857990506 0.28836800917167255 8 0.036093302077135306 0.1946862120029267 9 0.029065830662390584 0.17637776714282904 >10 0.17173619462509804 2.17256595287876 >50 0.015233677226662853 0.7111286586923111 >100 0.007729568349522464 1.0758821589167706 >500 0.0015348334840326184 0.7279412645218224 >1k 0.0017128163735966194 2.650826325332889 >5k 9.916282367081984E-4 5.015572095571265 >10k+ 7.469407497282534E-4 6.422249291132792 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24381 0.21151298279024675 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16810 0.14583213324736669 No Hit CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16322 0.14159857696987027 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16273 0.14117348627807247 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15521 0.13464964545701238 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15390 0.13351317850547134 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15346 0.13313146441487742 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15292 0.13266299712187574 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14496 0.12575744221022175 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14179 0.12300736569389724 No Hit CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14157 0.12281650864860026 No Hit CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14043 0.12182752214115232 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14020 0.12162798977561456 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13978 0.12126362632550218 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13820 0.1198929257274603 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13758 0.11935505587253248 No Hit TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13385 0.11611916142272476 No Hit ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13288 0.11527765535936994 No Hit TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13272 0.11513885023551758 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13090 0.1135599419516972 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13078 0.11345583810880794 No Hit CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12997 0.11275313716930548 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12872 0.11166872213920906 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12854 0.11151256637487517 No Hit TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12735 0.11048020326622336 No Hit ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12517 0.1085889834537352 No Hit TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12320 0.10687994536630326 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12300 0.10670643896148783 No Hit CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12257 0.10633340019113464 No Hit TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12221 0.10602108866246689 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12130 0.1052316345205567 No Hit ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12106 0.10502342683477818 No Hit GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12104 0.10500607619429664 No Hit GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12012 0.10420794673214567 No Hit GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11877 0.10303677849964155 No Hit GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11843 0.1027418176114553 No Hit CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11746 0.10190031154810049 No Hit ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11717 0.10164872726111812 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.415060633548227E-4 0.0 0.0 0.0 0.0 2 0.0019692976946551004 0.0 0.0 1.7350640481542736E-5 0.0 3 0.0028888816401768653 0.0 0.0 1.7350640481542736E-5 0.0 4 0.004866854655072738 0.0 0.0 1.7350640481542736E-5 0.0 5 0.00779911289645346 0.0 0.0 1.7350640481542736E-5 0.0 6 0.014305603077031985 0.0 0.0 1.7350640481542736E-5 0.0 7 0.021540820157835306 0.0 0.0 3.470128096308547E-5 0.0 8 0.04109499198053397 0.0 0.0 3.470128096308547E-5 0.0 9 0.1174985373410074 0.0 0.0 3.470128096308547E-5 0.0 10-11 0.2708434979168821 0.0 0.0 3.9038941083471155E-5 0.0 12-13 0.43774798402908244 0.0 0.0 4.337660120385684E-5 0.0 14-15 0.6113281290665564 4.337660120385684E-6 0.0 4.337660120385684E-5 0.0 16-17 0.7948979053612785 8.675320240771368E-6 0.0 5.205192144462821E-5 0.0 18-19 0.9926778562103842 8.675320240771368E-6 0.0 6.940256192617094E-5 0.0 20-21 1.2575397208212544 8.675320240771368E-6 0.0 7.807788216694231E-5 0.0 22-23 1.5598616182317755 8.675320240771368E-6 0.0 8.241554228732799E-5 0.0 24-25 1.8715962381035334 8.675320240771368E-6 0.0 8.675320240771368E-5 0.0 26-27 2.1611220381589167 8.675320240771368E-6 0.0 9.109086252809937E-5 0.0 28-29 2.4335834833007026 8.675320240771368E-6 0.0 9.976618276887072E-5 0.0 30-31 2.724445282673164 8.675320240771368E-6 0.0 1.0410384288925641E-4 0.0 32-33 3.0613730325241226 8.675320240771368E-6 0.0 1.0410384288925641E-4 0.0 34-35 3.394184342920835 8.675320240771368E-6 0.0 1.1711682325041346E-4 0.0 36-37 3.643634501123975 8.675320240771368E-6 0.0 1.2145448337079915E-4 0.0 38-39 3.8270134203733996 8.675320240771368E-6 0.0 1.3012980361157053E-4 0.0 40-41 3.9922522449993716 8.675320240771368E-6 0.0 1.344674637319562E-4 0.0 42-43 4.136431729740872 8.675320240771368E-6 0.0 1.6483108457465598E-4 0.0 44-45 4.2635338465884125 8.675320240771368E-6 0.0 1.9953236553774147E-4 0.0 46-47 4.381088773510985 8.675320240771368E-6 0.0 2.0820768577851283E-4 0.0 48-49 4.49922494688969 8.675320240771368E-6 0.0 2.168830060192842E-4 0.0 50-51 4.620553638116997 8.675320240771368E-6 0.0 2.47246626861984E-4 0.0 52-53 4.748011443094411 8.675320240771368E-6 0.0 3.513504697512404E-4 0.0 54-55 4.8703681597702495 8.675320240771368E-6 0.0 4.5111665252011113E-4 0.0 56-57 4.993336486523063 8.675320240771368E-6 0.0 4.988309138443537E-4 0.0 58-59 5.11655639756286 8.675320240771368E-6 0.0 5.07506234085125E-4 0.0 60-61 5.240908437894076 8.675320240771368E-6 0.0 5.638958156501389E-4 0.0 62-63 5.367902113238609 8.675320240771368E-6 0.0 6.723373186597809E-4 0.0 64-65 5.498821370992089 8.675320240771368E-6 0.0 8.024671222713515E-4 0.0 66-67 5.630569121828563 8.675320240771368E-6 0.0 9.456099062440792E-4 0.0 68-69 5.76088544482531 8.675320240771368E-6 0.0 0.001032363108651793 0.0 70-71 5.896168388659898 8.675320240771368E-6 0.0 0.0010800773699760353 0.0 72-73 6.034817356747906 8.675320240771368E-6 0.0 0.0011451422717818206 0.0 74-75 6.179144321933499 8.675320240771368E-6 0.0 0.0011711682325041346 0.0 76-77 6.3280258302454975 8.675320240771368E-6 0.0 0.0012926227158749338 0.0 78-79 6.480568323699101 8.675320240771368E-6 0.0 0.0016786744665892596 0.0 80-81 6.6362903220209475 8.675320240771368E-6 0.0 0.001977973014895872 0.0 82-83 6.801412029823669 8.675320240771368E-6 0.0 0.0020430379167016574 0.0 84-85 6.9715524103856765 8.675320240771368E-6 0.0 0.0020777391976647425 0.0 86-87 7.146555307942638 8.675320240771368E-6 0.0 0.002099427498266671 0.0 88 7.280883966550741 8.675320240771368E-6 0.0 0.002099427498266671 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGGC 600 0.0 66.5862 1 GCTTTCT 2090 0.0 66.117676 1 ACCGGGC 45 2.2051972E-7 62.665558 2 GCCTTTT 2930 0.0 53.899933 1 GCAAGCC 105 0.0 53.716595 1 AGGCCGC 35 2.9388844E-4 53.71333 2 AGGCATA 710 0.0 52.29462 4 AACGGGC 45 1.6150392E-5 52.2213 2 GGTCCCG 45 1.6150807E-5 52.22107 3 GCTTTTC 2150 0.0 51.374306 1 GGCATAT 735 0.0 51.165546 5 GACTGCG 65 4.8070433E-8 50.617565 1 CTAGCTT 410 0.0 50.441196 1 GCCAATG 255 0.0 49.76382 2 GCATATC 785 0.0 47.905346 6 GGCTGCC 60 1.6059348E-6 47.002026 1 GAGGTCC 40 5.6776596E-4 47.00202 1 AGCTCCG 50 3.0103649E-5 47.00202 1 GTGGTCC 50 3.0103649E-5 47.00202 1 GCCGTCC 40 5.6776596E-4 47.00202 1 >>END_MODULE