FastQCFastQC Report
Fri 27 May 2016
SRR2032146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42469573
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC3794860.8935479525541733No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1837560.4326768248882559No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT1143580.2692704256762836No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT995860.23448787676768024No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA984940.23191662416761288No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC938200.2209110979288631No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT934420.2200210489519167No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC874590.20593331607077847No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC759850.17891632675468624No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT682010.1605879107849754No Hit
GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA643030.15140957503858116No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC619490.1458667832615129No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG617560.14541234026534713No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA608170.14320134558452002No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC607400.14302003931143834No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG592050.13940568698442057No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG582960.1372653311112876No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA536820.12640108248792611No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC534840.12593486635714468No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG521390.12276789314552326No Hit
CCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTC516280.12156467878779945No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA507060.11939371276466566No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA495100.1165775789645919No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC493270.11614668223765753No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG483400.11382266546451975No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT481400.11335174007989202No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA475930.1120637591529352No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT468160.11023421403365652No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT464390.10934651968363326No Hit
CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT463650.10917227729132101No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT458820.10803499248744507No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG453470.10677526708356593No Hit
GTCCAATGTACTGAGAGGCATGGGTGGTGCTTTTGTATTGGTATTGTATG452310.10650213036048184No Hit
CCCATTTATTATCATTGGCCAACTAGCCTCCATCTCATACTTCTCAATCA447290.10532010764506627No Hit
CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA441050.10385082044502779No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC440630.10375192611425596No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA434200.10223790100267784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA312850.060.411041
CCAAACG336450.055.409158
CCGAAAC359750.052.2057652
GTCCGTA15750.040.462141
CGAAACC464800.040.0121083
ACTCGTC277350.039.94500442-43
TCCGCTC44400.038.862962
CTAACGA278550.038.44262394-95
CAAACGA491900.037.9084329
TCGTCTA297400.037.28366544-45
CTCCGCT60350.034.9889031
CGAGCTA326150.034.6960412-13
GATATAT211450.034.4340065
TACGCAT160050.034.0087979
CAACTCG344700.032.39135440-41
TAGGTAG335650.032.3631976-77
CTACGCA174950.031.3024028
CCGCTCA44950.031.297513
CCAGATA232200.031.2395322
AAACCAA619550.030.7382745
Produced by FastQC (version 0.11.5)