FastQCFastQC Report
Fri 27 May 2016
SRR2032145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29550268
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1542320.5219309686125351No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1042980.35295111367517884No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1025630.3470797625253348No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG916770.31024084113213457No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC860160.2910836544697327No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT821850.2781193050431895No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA786040.2660009716324739No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA737560.24959502905354364No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG685030.23181854052897252No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG657430.222478523714235No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG638460.21605895418613463No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC622640.2107053648379771No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT607110.20544991334765558No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG602150.2037714175722535No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA587920.1989558944101624No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT569820.19283073845557003No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA517850.17524375751854432No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT516040.1746312419230851No Hit
ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT494760.16742995359635995No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC491360.1662793718148343No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA487730.16505095655985252No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA466740.15794780609096337No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT460600.1558699907560906No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA450640.15249946294903316No Hit
GTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTA449360.15206630274892938No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG447570.15146055528159677No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC445440.1507397496361116No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA415010.14044204269145713No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG408900.13837437954877432No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA402430.13618489009981227No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT396950.13433042299311804No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT384190.1300123572483336No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA384080.12997513254363716No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT375830.12718327969140586No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT373350.1263440318037048No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT369790.12513930499716616No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA362030.12251327128403708No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC355760.12039146311634127No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA344440.11656070259667356No Hit
TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA341860.11568761406833943No Hit
CTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTGTGACTAGA337870.11433737250707846No Hit
CTCGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAG327990.1109939172125275No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA326880.1106182861015No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT312360.10570462508157287No Hit
CTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTC311670.10547112466120442No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT306730.10379939701392896No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT303290.10263527897615007No Hit
TTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTG301030.10187048049784184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT570750.087.757321
GCGTGTT616550.080.4056852
CGAACGT107100.047.4904861
CGTGTTT1111450.044.4448663
CGCAGGA164200.038.7921561
TCGAACG66800.035.617012
ACGTTTA154100.034.4847074
GAACGTT151300.034.24382
CACGCGT5600.033.953481
GTAAACG37150.033.7798271
CGCGTTT9650.033.0034831
TCGCAGG100850.032.962362
GTCGAAC72550.032.956641
CGCGTGA4050.031.6899131
GTTTTTT3939950.031.6714121
AACGTTT166300.031.3262983
GCAGGAT208550.030.5134092
CAGGATA211350.030.1989383
GTGTTTT1666700.029.1799134
TAAACGC43200.029.0212652
Produced by FastQC (version 0.11.5)