##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2032145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29550268 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33492298614686 34.0 31.0 34.0 30.0 34.0 2 32.50066280278744 34.0 31.0 34.0 31.0 34.0 3 32.56717424694761 34.0 31.0 34.0 31.0 34.0 4 35.88158618392226 37.0 35.0 37.0 35.0 37.0 5 36.04147211118356 37.0 37.0 37.0 35.0 37.0 6 36.04036690970112 37.0 36.0 37.0 35.0 37.0 7 36.105616131806315 37.0 37.0 37.0 35.0 37.0 8 36.11031927020087 37.0 37.0 37.0 35.0 37.0 9 37.92245099096902 39.0 39.0 39.0 35.0 39.0 10-11 37.85478172313023 39.0 38.5 39.0 35.0 39.0 12-13 37.82273145543046 39.0 38.5 39.0 35.0 39.0 14-15 39.22067248256429 41.0 39.0 41.0 36.0 41.0 16-17 39.054218340760904 41.0 39.0 41.0 36.0 41.0 18-19 38.84114861496349 41.0 39.0 41.0 35.5 41.0 20-21 38.61715866671666 41.0 39.0 41.0 34.5 41.0 22-23 38.404457177850304 41.0 39.0 41.0 34.0 41.0 24-25 38.28748389016303 41.0 39.0 41.0 34.0 41.0 26-27 38.11231067345988 41.0 39.0 41.0 34.0 41.0 28-29 37.924523831729715 41.0 39.0 41.0 33.0 41.0 30-31 37.779807310038606 41.0 39.0 41.0 33.0 41.0 32-33 37.68573540517467 41.0 39.0 41.0 33.0 41.0 34-35 37.583502203770195 40.5 38.0 41.0 32.5 41.0 36-37 37.50654088822477 40.5 38.0 41.0 32.0 41.0 38-39 37.4447865921216 40.0 38.0 41.0 32.0 41.0 40-41 37.3950818821677 40.0 38.0 41.0 32.0 41.0 42-43 37.27905212230224 40.0 38.0 41.0 31.5 41.0 44-45 37.21396978531633 40.0 38.0 41.0 31.0 41.0 46-47 37.14319318525301 40.0 38.0 41.0 31.0 41.0 48-49 37.05375599300825 40.0 38.0 41.0 31.0 41.0 50-51 36.96029547007831 40.0 38.0 41.0 31.0 41.0 52-53 36.8469618109724 40.0 38.0 41.0 30.5 41.0 54-55 36.722722633175444 40.0 37.0 41.0 30.0 41.0 56-57 36.60527430749528 40.0 37.0 41.0 30.0 41.0 58-59 36.40592968564617 40.0 37.0 41.0 30.0 41.0 60-61 36.27294117941672 40.0 36.0 41.0 29.5 41.0 62-63 36.06463147813076 39.0 36.0 41.0 29.0 41.0 64-65 35.817394989446456 39.0 35.0 41.0 29.0 41.0 66-67 35.60300832127817 39.0 35.0 41.0 29.0 41.0 68-69 35.30031736091192 38.5 35.0 40.5 28.0 41.0 70-71 35.18709133196356 38.0 35.0 40.0 29.0 41.0 72-73 34.94320640340724 37.0 35.0 40.0 29.0 41.0 74-75 34.59969347824527 37.0 35.0 39.0 28.5 41.0 76-77 34.23664616172009 36.0 34.5 39.0 29.0 41.0 78-79 33.910883667789406 36.0 34.0 39.0 28.0 40.0 80-81 33.76122879833103 35.5 34.0 37.5 29.0 39.0 82-83 33.48105013802244 35.0 34.0 37.0 29.0 39.0 84-85 33.21313055434895 35.0 34.0 37.0 29.0 39.0 86-87 32.930075896435184 35.0 34.0 36.0 29.0 37.0 88-89 32.70379395543892 35.0 34.0 36.0 29.0 37.0 90-91 32.515468252267624 35.0 34.0 36.0 29.0 36.5 92-93 32.354331896414614 35.0 34.0 35.0 29.0 36.0 94-95 32.18258382631251 35.0 34.0 35.0 28.0 36.0 96-97 32.07308622378653 35.0 34.0 35.0 28.0 36.0 98-99 31.954196608301487 35.0 34.0 35.0 27.0 36.0 100-101 31.621834360351656 35.0 33.5 35.0 26.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25196567625531685 1101 2 0.23022727994059267 1101 3 0.21096876649378515 1101 4 0.35015017115039626 1101 5 0.2335489013135401 1101 6 0.19885562821266944 1101 7 0.1130918192310375 1101 8 0.10857819480267494 1101 9 0.1619067423005518 1101 10-11 0.2590342257904936 1101 12-13 0.22548497786817023 1101 14-15 0.2765480378311196 1101 16-17 0.2926945724598937 1101 18-19 0.35769835313448795 1101 20-21 0.34544872117814407 1101 22-23 0.32415585957544835 1101 24-25 0.30005170256676905 1101 26-27 0.2920515911598045 1101 28-29 0.29699365121845034 1101 30-31 0.30660418148406166 1101 32-33 0.3334552259020711 1101 34-35 0.33024123400015526 1101 36-37 0.34923906533912685 1101 38-39 0.3428787457456437 1101 40-41 0.3229426743879529 1101 42-43 0.33331509451679153 1101 44-45 0.36471162614154196 1101 46-47 0.35362133404385787 1101 48-49 0.30613099489332285 1101 50-51 0.28683548341615506 1101 52-53 0.27235406373341675 1101 54-55 0.3014356499281732 1101 56-57 0.2900490437548271 1101 58-59 0.3494013335321995 1101 60-61 0.3198353197475683 1101 62-63 0.2662738448357018 1101 64-65 0.2684325721264784 1101 66-67 0.2878816387529426 1101 68-69 0.2899591910392516 1101 70-71 0.3215682361693055 1101 72-73 0.2932787757711566 1101 74-75 0.282846150245156 1101 76-77 0.268972636959937 1101 78-79 0.22838216376747766 1101 80-81 0.2637973314123343 1101 82-83 0.25269407800431054 1101 84-85 0.22505282075383093 1101 86-87 0.25430787104167507 1101 88-89 0.2162920228281635 1101 90-91 0.2614860964642318 1101 92-93 0.26584951079635033 1101 94-95 0.2826008675834757 1101 96-97 0.2470876830542963 1101 98-99 0.27506609535525683 1101 100-101 0.21359655358197926 1102 1 0.21095124564617862 1102 2 0.21610732918453124 1102 3 0.188548053859094 1102 4 0.33807181365421 1102 5 0.22305387948290445 1102 6 0.20599607350349913 1102 7 0.16130501258196261 1102 8 0.15946729543181704 1102 9 0.17612465346873307 1102 10-11 0.2299015362588932 1102 12-13 0.22271119453716182 1102 14-15 0.27119277786239593 1102 16-17 0.2761529364209636 1102 18-19 0.3178028260862291 1102 20-21 0.2782867681083019 1102 22-23 0.26394841497187116 1102 24-25 0.27941704548718604 1102 26-27 0.2578700894747783 1102 28-29 0.2835153877814207 1102 30-31 0.27356167863830194 1102 32-33 0.24426291741797002 1102 34-35 0.24121160432765976 1102 36-37 0.2589933368474675 1102 38-39 0.26603187488966284 1102 40-41 0.2839403023962319 1102 42-43 0.28703035229571583 1102 44-45 0.25481112884925494 1102 46-47 0.22430443335382932 1102 48-49 0.20131183693284527 1102 50-51 0.23083983481795656 1102 52-53 0.24079831401000718 1102 54-55 0.25093861442572774 1102 56-57 0.24196789050193246 1102 58-59 0.29554779602543135 1102 60-61 0.29696442433360914 1102 62-63 0.27337353865428327 1102 64-65 0.26500009937316804 1102 66-67 0.2288369379176629 1102 68-69 0.2759552609900524 1102 70-71 0.27184130951303587 1102 72-73 0.2637821704864578 1102 74-75 0.26891255663026215 1102 76-77 0.2927721390653062 1102 78-79 0.2492347283744749 1102 80-81 0.2526429053251604 1102 82-83 0.23865830453122783 1102 84-85 0.23473143971063593 1102 86-87 0.23217496725219178 1102 88-89 0.23674049148521448 1102 90-91 0.22216021387443874 1102 92-93 0.23836393257735722 1102 94-95 0.23165955593425736 1102 96-97 0.2750066017880215 1102 98-99 0.2680752798209909 1102 100-101 0.21294357691374088 1103 1 0.18324402718457833 1103 2 0.17368876389788568 1103 3 0.14055185259282155 1103 4 0.3100938794573551 1103 5 0.1850931713438726 1103 6 0.17502249185663032 1103 7 0.13610878944582794 1103 8 0.12881699473427943 1103 9 0.17785171063009386 1103 10-11 0.20928272813903703 1103 12-13 0.16369799773818272 1103 14-15 0.24068292176643524 1103 16-17 0.2789529712267935 1103 18-19 0.280095414292191 1103 20-21 0.2421817239398365 1103 22-23 0.2739649873420049 1103 24-25 0.25959169776799484 1103 26-27 0.23483902156943515 1103 28-29 0.2376523694018715 1103 30-31 0.2732779289897991 1103 32-33 0.270956765075411 1103 34-35 0.25670997023422615 1103 36-37 0.26021305423007846 1103 38-39 0.27401255725885676 1103 40-41 0.2955604840450903 1103 42-43 0.2321498103528512 1103 44-45 0.22553199067361618 1103 46-47 0.24112363633675926 1103 48-49 0.2176854335142977 1103 50-51 0.24765763738388102 1103 52-53 0.21929062901273255 1103 54-55 0.2422038825358186 1103 56-57 0.2128679457924889 1103 58-59 0.20543732286409977 1103 60-61 0.23759642057733998 1103 62-63 0.24183573184044604 1103 64-65 0.20765860207497866 1103 66-67 0.19233457672172705 1103 68-69 0.26676675115309934 1103 70-71 0.25394021893836083 1103 72-73 0.24400082042478743 1103 74-75 0.217290370482921 1103 76-77 0.21095612046589451 1103 78-79 0.19600427697372425 1103 80-81 0.20718271547521994 1103 82-83 0.16609227212835265 1103 84-85 0.19128502914927026 1103 86-87 0.18992758115225428 1103 88-89 0.17367386508984595 1103 90-91 0.18472347527892197 1103 92-93 0.20950921062163275 1103 94-95 0.18651243013035668 1103 96-97 0.18182453813495414 1103 98-99 0.20106416369121405 1103 100-101 0.14380149759565697 1104 1 0.06953803933185299 1104 2 0.06835622117644391 1104 3 0.013414457979230576 1104 4 0.22832076700842663 1104 5 0.12503484571616497 1104 6 0.0909086256045768 1104 7 0.05629492376662881 1104 8 0.06323011160369418 1104 9 0.07843838252242108 1104 10-11 0.15163284743494643 1104 12-13 0.099930198826506 1104 14-15 0.07875923522682626 1104 16-17 0.11334155462246542 1104 18-19 0.15875922669638243 1104 20-21 0.08510384779372515 1104 22-23 0.14281179413561773 1104 24-25 0.09596025255460461 1104 26-27 0.14582514202250252 1104 28-29 0.12049898393286185 1104 30-31 0.1034910033490064 1104 32-33 0.13810718545147438 1104 34-35 0.156679519105694 1104 36-37 0.19548516252154968 1104 38-39 0.19006391453029892 1104 40-41 0.18665492258335092 1104 42-43 0.1472357956611674 1104 44-45 0.17267813675692878 1104 46-47 0.15068733505419374 1104 48-49 0.14416929037639648 1104 50-51 0.12342335398759019 1104 52-53 0.1278169145705732 1104 54-55 0.1556366832304903 1104 56-57 0.11801874723283134 1104 58-59 0.16492435641541903 1104 60-61 0.158580725216801 1104 62-63 0.15088040264530633 1104 64-65 0.14407305284834848 1104 66-67 0.1035398276274293 1104 68-69 0.13959581202360738 1104 70-71 0.1642179745712653 1104 72-73 0.15868578541652312 1104 74-75 0.13345623022740227 1104 76-77 0.14635881457714106 1104 78-79 0.16337217906992407 1104 80-81 0.16470069041878332 1104 82-83 0.1571575938000649 1104 84-85 0.17828002948582622 1104 86-87 0.16517498530856756 1104 88-89 0.1294589636987311 1104 90-91 0.08748677660587845 1104 92-93 0.09269667836901618 1104 94-95 0.0752687666813543 1104 96-97 0.13531751786419477 1104 98-99 0.09946369018335588 1104 100-101 0.02776625231343033 1105 1 0.08560170593724337 1105 2 0.07261349428559072 1105 3 0.029122754094530023 1105 4 0.2496697569367825 1105 5 0.11060225152752423 1105 6 0.10687148785380174 1105 7 0.10014551234745994 1105 8 0.10631821608873082 1105 9 0.13329073368422684 1105 10-11 0.1698283070555533 1105 12-13 0.14670686627622587 1105 14-15 0.18038754436256 1105 16-17 0.20400503094701605 1105 18-19 0.21835282426243907 1105 20-21 0.2027155871929196 1105 22-23 0.21008294021009277 1105 24-25 0.20322328997085748 1105 26-27 0.22520560995033634 1105 28-29 0.2135007305152925 1105 30-31 0.21685892904501713 1105 32-33 0.22168302090939562 1105 34-35 0.2534009116071161 1105 36-37 0.31376703488074753 1105 38-39 0.27921731543644057 1105 40-41 0.26247958488347223 1105 42-43 0.24044266271979353 1105 44-45 0.2474137636040865 1105 46-47 0.2192371181934476 1105 48-49 0.23038609747357697 1105 50-51 0.23084856993887826 1105 52-53 0.23811118393106057 1105 54-55 0.24004643088522215 1105 56-57 0.20605412989311844 1105 58-59 0.25864118732667407 1105 60-61 0.2573804968164737 1105 62-63 0.2803347804261378 1105 64-65 0.2555725909965716 1105 66-67 0.28381702886582616 1105 68-69 0.2971090323701375 1105 70-71 0.31043012569820405 1105 72-73 0.25858343971712117 1105 74-75 0.2575219720070976 1105 76-77 0.2710797620591734 1105 78-79 0.22020111679556464 1105 80-81 0.21698012619501128 1105 82-83 0.2281398760412401 1105 84-85 0.23314694581824824 1105 86-87 0.22070493701644267 1105 88-89 0.19503533151789298 1105 90-91 0.23315914921985126 1105 92-93 0.2284166853574945 1105 94-95 0.23092122111570035 1105 96-97 0.22390202687570593 1105 98-99 0.22116827503359104 1105 100-101 0.14905031842266325 1106 1 0.049019899036451875 1106 2 0.06361323171506683 1106 3 0.038657334494303086 1106 4 0.210860107639796 1106 5 0.0758372260507798 1106 6 0.051649847089130674 1106 7 0.0026368692264995275 1106 8 -0.001074114686147709 1106 9 -0.009051251244812875 1106 10-11 0.054840179783113285 1106 12-13 0.0654364535340548 1106 14-15 0.02099608957085053 1106 16-17 0.05216305959874745 1106 18-19 0.0919201076915428 1106 20-21 0.07398902231499704 1106 22-23 0.08295593180282168 1106 24-25 0.10487764649522546 1106 26-27 0.06400192291995666 1106 28-29 0.06558058851169335 1106 30-31 0.11008315809174007 1106 32-33 0.07092178636636959 1106 34-35 0.06541974470457035 1106 36-37 0.0473995219481651 1106 38-39 0.03684753948422781 1106 40-41 0.038927290322526176 1106 42-43 0.08571341935485322 1106 44-45 0.04222982734979297 1106 46-47 0.04350183805897956 1106 48-49 0.10636925359016658 1106 50-51 0.08637625293106765 1106 52-53 0.07585652340570448 1106 54-55 0.11287890514655174 1106 56-57 0.08352758956467454 1106 58-59 0.08225377427687874 1106 60-61 0.06974412809661601 1106 62-63 0.095069948223518 1106 64-65 0.08960635872022493 1106 66-67 0.10509787818121197 1106 68-69 0.13076256482786164 1106 70-71 0.12644862561054282 1106 72-73 0.11951058716604024 1106 74-75 0.08035648710156096 1106 76-77 0.09947286656601761 1106 78-79 0.0834444507173302 1106 80-81 0.09074619204373846 1106 82-83 0.09894455119447798 1106 84-85 0.05938436459575769 1106 86-87 0.06637109016469367 1106 88-89 0.05436518329851481 1106 90-91 0.036575184538556016 1106 92-93 0.05206313702332466 1106 94-95 0.04699108242326844 1106 96-97 0.06931853752804784 1106 98-99 0.07378402268608397 1106 100-101 0.005936473233603579 1107 1 0.06544022379137715 1107 2 0.03443371471322365 1107 3 -0.013464229353189694 1107 4 0.20860613912191184 1107 5 0.07845522526879734 1107 6 0.11918055455067389 1107 7 0.06732729270002125 1107 8 0.06777446387603447 1107 9 0.08315723553840826 1107 10-11 0.09793640953773064 1107 12-13 0.08992134026254206 1107 14-15 0.07396369259357272 1107 16-17 0.09789113485454237 1107 18-19 0.14820192342975957 1107 20-21 0.14469439233193526 1107 22-23 0.14500254652316613 1107 24-25 0.11963136726321721 1107 26-27 0.1725442179509571 1107 28-29 0.10908610505406813 1107 30-31 0.16076052412304875 1107 32-33 0.1531195159876404 1107 34-35 0.1500052132725216 1107 36-37 0.15644089149638774 1107 38-39 0.16353354449834256 1107 40-41 0.17259101843129798 1107 42-43 0.16532279001469874 1107 44-45 0.11241876650123572 1107 46-47 0.14915184603579945 1107 48-49 0.1497416278206387 1107 50-51 0.14673685142092552 1107 52-53 0.16608984772634727 1107 54-55 0.17584943498296468 1107 56-57 0.19471704374559096 1107 58-59 0.20027078806391785 1107 60-61 0.18538470376312688 1107 62-63 0.18480010339082753 1107 64-65 0.1487832735234278 1107 66-67 0.1644574931930478 1107 68-69 0.23777777040353243 1107 70-71 0.17495736257168204 1107 72-73 0.21546284341873445 1107 74-75 0.2235720867810116 1107 76-77 0.22100102487575413 1107 78-79 0.2171111082789352 1107 80-81 0.22737122881035532 1107 82-83 0.20046544393335353 1107 84-85 0.20261830634477462 1107 86-87 0.20991806902129895 1107 88-89 0.21996719311429302 1107 90-91 0.2143995353381527 1107 92-93 0.1921299509807639 1107 94-95 0.20519948448988146 1107 96-97 0.19802599827700362 1107 98-99 0.17818098888137968 1107 100-101 0.09034450273187389 1108 1 0.06398557035597463 1108 2 0.04481999329535569 1108 3 0.02843976263052639 1108 4 0.21523660961877766 1108 5 0.08336882035067816 1108 6 0.07679701658177152 1108 7 0.04681822689993709 1108 8 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2214 54-55 -0.3013497685037052 2214 56-57 -0.26809972099658097 2214 58-59 -0.216836509929621 2214 60-61 -0.30322645641650325 2214 62-63 -0.29920243514040124 2214 64-65 -0.24877675015800804 2214 66-67 -0.2796793626766103 2214 68-69 -0.2576111879426577 2214 70-71 -0.2842585452291857 2214 72-73 -0.31806803087923896 2214 74-75 -0.23420746259831304 2214 76-77 -0.2589375406287928 2214 78-79 -0.3256951372526089 2214 80-81 -0.26896465016327653 2214 82-83 -0.24576008730873866 2214 84-85 -0.23750532667197888 2214 86-87 -0.2552718007430954 2214 88-89 -0.1955848129524398 2214 90-91 -0.20906837614706575 2214 92-93 -0.19472621437100202 2214 94-95 -0.2415152326745158 2214 96-97 -0.26018135024759204 2214 98-99 -0.1999016064388286 2214 100-101 -0.21811795561842828 2215 1 -0.4544190980202032 2215 2 -0.36275405749228895 2215 3 -0.2624657549991056 2215 4 -2.432382913175161 2215 5 -0.6900082264109244 2215 6 -0.3899097329977934 2215 7 -0.22292690056067954 2215 8 -0.15816250471646498 2215 9 -0.21055246542037764 2215 10-11 -0.16046484911333891 2215 12-13 -0.24509395962551395 2215 14-15 -0.2888771847666334 2215 16-17 -0.27203822821604007 2215 18-19 -0.236863221659938 2215 20-21 -0.26102457789725264 2215 22-23 -0.28907876669483556 2215 24-25 -0.3450908645990509 2215 26-27 -0.3210963953667729 2215 28-29 -0.3489039748263991 2215 30-31 -0.36276600143231974 2215 32-33 -0.35911120817036135 2215 34-35 -0.38419565598302086 2215 36-37 -0.39907189276826216 2215 38-39 -0.40581781248702953 2215 40-41 -0.395237034163074 2215 42-43 -0.39125793210948245 2215 44-45 -0.35352481561681515 2215 46-47 -0.37656471995957475 2215 48-49 -0.3930449969221499 2215 50-51 -0.3684286569926627 2215 52-53 -0.3674383826564309 2215 54-55 -0.3795600002052808 2215 56-57 -0.37049043813965454 2215 58-59 -0.3331016546413963 2215 60-61 -0.3578922474440489 2215 62-63 -0.34907556980729737 2215 64-65 -0.35540995731071234 2215 66-67 -0.38495695941486474 2215 68-69 -0.3408455036209972 2215 70-71 -0.3440429636838118 2215 72-73 -0.35985579318828087 2215 74-75 -0.3577943533634098 2215 76-77 -0.3342658525424298 2215 78-79 -0.3338954312552005 2215 80-81 -0.313746039985233 2215 82-83 -0.31989201050730287 2215 84-85 -0.3149632980025956 2215 86-87 -0.3145783093661976 2215 88-89 -0.3412350875088137 2215 90-91 -0.3279623217404364 2215 92-93 -0.33469950210122335 2215 94-95 -0.360317216294753 2215 96-97 -0.33867945538814226 2215 98-99 -0.3049837916665865 2215 100-101 -0.20644654363033155 2216 1 -0.5336168285605893 2216 2 -0.5395852252173441 2216 3 -0.47957543672117 2216 4 -0.8104968690216694 2216 5 -0.5138087148559052 2216 6 -0.4527332413852463 2216 7 -0.3709299954950396 2216 8 -0.31442425381222705 2216 9 -0.5063736720667436 2216 10-11 -0.43983550954712314 2216 12-13 -0.5101665956240922 2216 14-15 -0.6675948966347107 2216 16-17 -0.5665430537897151 2216 18-19 -0.6128862833863806 2216 20-21 -0.6959809097316736 2216 22-23 -0.6761130388008425 2216 24-25 -0.681618763718312 2216 26-27 -0.6413251047577546 2216 28-29 -0.6471578661136661 2216 30-31 -0.7399172398132734 2216 32-33 -0.7046843133968537 2216 34-35 -0.7226258745019436 2216 36-37 -0.790175568555135 2216 38-39 -0.7699728096191336 2216 40-41 -0.7690183904185872 2216 42-43 -0.783523069258024 2216 44-45 -0.7068526077163284 2216 46-47 -0.7536898207266134 2216 48-49 -0.8324664401773063 2216 50-51 -0.8709674738954902 2216 52-53 -0.8535814618927162 2216 54-55 -0.7869424274654548 2216 56-57 -0.7297650502778268 2216 58-59 -0.7671810880700889 2216 60-61 -0.7529071129851914 2216 62-63 -0.7428046924059615 2216 64-65 -0.7607646856920027 2216 66-67 -0.8196306521305061 2216 68-69 -0.7288413991098892 2216 70-71 -0.745807949970704 2216 72-73 -0.8230519934448992 2216 74-75 -0.8850858727404116 2216 76-77 -0.7918813327988659 2216 78-79 -0.8839949019273945 2216 80-81 -0.7037611392555903 2216 82-83 -0.6397212170375042 2216 84-85 -0.6063377144297846 2216 86-87 -0.6047338679326941 2216 88-89 -0.7197782538210333 2216 90-91 -0.6199317802058566 2216 92-93 -0.6403674717592835 2216 94-95 -0.6570679465840001 2216 96-97 -0.6972630323961297 2216 98-99 -0.7283366864208389 2216 100-101 -0.7202526859850735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50380.0 3 15005.0 4 17391.0 5 32851.0 6 110217.0 7 118189.0 8 93808.0 9 120585.0 10 179178.0 11 195380.0 12 151505.0 13 104156.0 14 78138.0 15 66402.0 16 57484.0 17 49169.0 18 44059.0 19 40898.0 20 41996.0 21 47514.0 22 59899.0 23 78982.0 24 101957.0 25 121963.0 26 142309.0 27 165492.0 28 196258.0 29 232260.0 30 281390.0 31 348078.0 32 441410.0 33 584245.0 34 816320.0 35 1227208.0 36 2256018.0 37 5567382.0 38 1.0134632E7 39 5163043.0 40 17117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.94240039410868 19.286219364017345 14.04423105698779 37.72714918488618 2 18.147703207978765 21.677812186659683 51.049023062659415 9.125461542702135 3 13.50557204400781 22.53259129657894 46.21892565982951 17.742910999583746 4 18.032183031698565 29.339170989670986 39.168295742940515 13.460350235689939 5 18.15216109363089 32.881764442613225 38.031159816949824 10.934914646806055 6 16.001337111392697 34.843321894745586 37.27940132387293 11.87593966998878 7 13.669145064944926 17.833608141895702 55.336838231044126 13.160408562115242 8 14.016905024346988 21.62579371530573 48.320380715328874 16.036920545018408 9 15.886228172279182 22.485741922882053 46.1909144106578 15.437115494180967 10-11 17.510531848915864 30.10662839767621 38.34607057140387 14.036769182004052 12-13 17.888962539546103 23.670047499738793 42.63591105463464 15.805078906080466 14-15 18.31632081036073 26.078929167921483 41.24909258215374 14.355657439564052 16-17 19.34232105497207 26.817614183615664 38.75762053250829 15.082444228903977 18-19 19.48505915386075 27.87604050275714 35.749202426034024 16.889697917348084 20-21 20.87837426804894 27.35538845175424 34.95930839027496 16.806928889921853 22-23 20.720835126870774 29.21453322613875 33.48853343903663 16.576098207953848 24-25 20.534962897240003 29.511439983117793 32.34508270000953 17.608514419632673 26-27 20.992597802925577 29.762687038439367 31.83133934024207 17.413375818392986 28-29 21.707253363617983 29.362802271429967 31.157941790901216 17.772002574050834 30-31 21.052956572921953 30.801591350408543 30.12857451879425 18.01687755787525 32-33 21.12105267219297 29.45038326778004 31.35808153427871 18.070482525748275 34-35 21.96417270835374 29.64784287500986 30.18606501488572 18.201919401750676 36-37 21.992058314612922 30.027994737131568 30.023427379607654 17.95651956864786 38-39 22.281894727479212 28.574355047867485 31.007826701071423 18.13592352358188 40-41 22.319876432668362 29.503766743392223 29.48126235568816 18.695094468251256 42-43 22.3245935262769 29.50713450695791 29.803328236702892 18.364943730062297 44-45 22.306939683281616 29.056425410003424 30.000659156568975 18.635975750145985 46-47 21.108323861887243 29.9926756858053 29.55905218588249 19.339948266424965 48-49 21.349281978251707 29.98214715041354 29.966754720280363 18.701816151054388 50-51 21.367163627095483 29.757086544867178 30.268674723652563 18.607075104384776 52-53 22.617409027372318 28.724874686208857 29.349013769771936 19.30870251664689 54-55 22.23815142196442 28.52112203479955 29.888911814134033 19.35181472910199 56-57 22.729454838166983 28.134857407469465 30.009379907229977 19.126307847133578 58-59 21.97513183980615 28.738051396070862 29.245025106984173 20.041791657138813 60-61 22.25357843173181 28.672426027334446 29.11514550224362 19.95885003869012 62-63 22.57638774580142 28.58056590975352 29.268845004673672 19.574201339771392 64-65 22.406715929081134 28.405976471547234 29.427095763271222 19.760211836100407 66-67 22.321659692044697 28.48746472138889 29.046523337832635 20.144352248733778 68-69 22.899611119649087 28.146686223520895 29.181025699006334 19.77267695782368 70-71 22.20909306054587 27.718800269313782 29.5003585445397 20.571748125600642 72-73 22.653053179153982 27.434548109227407 29.490237805587004 20.422160906031603 74-75 23.494605197082734 27.271068797320595 29.585233796684506 19.649092208912162 76-77 22.511505344260996 27.665958479249262 29.34342033076059 20.47911584572915 78-79 22.186637046704018 28.283957569261315 28.949507878684138 20.579897505350527 80-81 22.272491010373642 27.524139778588395 30.07071955639542 20.132649654642545 82-83 22.292003526468136 28.32438949912029 28.704632021418817 20.678974952992753 84-85 21.756121471924224 28.394571031089683 29.02224585919982 20.827061637786272 86-87 22.084228120148644 29.239143996688654 28.992687347399166 19.683940535763533 88-89 22.39311419351869 27.238335949511 30.31215017979877 20.056399677171534 90-91 22.604912227958472 27.50733733031207 29.155354702239304 20.732395739490155 92-93 22.364770407477057 27.516956351873052 29.98163211062493 20.136641130024962 94-95 22.948439319494483 27.889902140706123 28.68552671847633 20.476131821323065 96-97 22.11009254452393 27.95843563042552 29.370408904301858 20.56106292074869 98-99 24.256319647266274 26.803666829441397 29.0567617020307 19.883251821261634 100-101 24.35404255146081 27.28095204565923 28.48244612647018 19.88255927640978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 725.0 1 1653.0 2 2368.5 3 2627.0 4 3072.0 5 3843.5 6 4487.0 7 4741.0 8 5561.5 9 6660.0 10 8235.0 11 10187.0 12 12409.5 13 15084.0 14 18133.5 15 21876.5 16 26913.0 17 32982.5 18 41850.5 19 52016.0 20 69395.5 21 94923.0 22 119812.0 23 145513.0 24 178851.5 25 231178.0 26 286219.5 27 386373.5 28 529234.0 29 702411.5 30 905370.0 31 966952.0 32 1041772.5 33 1183079.5 34 1385954.0 35 1376545.5 36 1220949.5 37 1325408.0 38 1379624.5 39 1419078.5 40 1425820.0 41 1266209.5 42 1034896.5 43 858006.0 44 786470.5 45 730741.5 46 652787.0 47 607023.5 48 628584.5 49 639489.0 50 577508.0 51 530095.5 52 545846.0 53 546674.5 54 608206.0 55 555648.5 56 418014.5 57 423239.5 58 384002.0 59 282370.5 60 210036.5 61 148991.0 62 103447.5 63 80451.0 64 66549.0 65 54148.0 66 39996.5 67 23534.5 68 11338.0 69 6711.5 70 3909.0 71 4098.0 72 9691.5 73 15154.5 74 12789.0 75 7517.0 76 5231.0 77 8841.5 78 8397.0 79 2535.0 80 670.0 81 231.5 82 123.5 83 69.5 84 49.0 85 45.5 86 33.0 87 24.5 88 24.5 89 21.5 90 15.0 91 15.0 92 17.0 93 17.5 94 14.0 95 11.5 96 12.0 97 11.5 98 15.0 99 16.0 100 65.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10662170644273007 2 0.002460214574026875 3 0.0032757740132847526 4 0.007908557715957094 5 0.00336037561486752 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0016734196793071387 12-13 3.570187586792783E-4 14-15 0.002575272752179439 16-17 0.0061183878264657356 18-19 0.0032859262054746844 20-21 0.007108226564984115 22-23 0.003010124984314863 24-25 0.0067495157742731805 26-27 0.012072648545860905 28-29 0.01037554041811059 30-31 0.010698718536156763 32-33 0.012940660978100097 34-35 0.01011327545320401 36-37 0.012328145382640862 38-39 0.016047231788219315 40-41 0.016644519095393655 42-43 0.018898305761558574 44-45 0.016702048184469936 46-47 0.020998117512842862 48-49 0.022081018013102284 50-51 0.027899916169965025 52-53 0.022106398493577116 54-55 0.02499977326770776 56-57 0.02841937000368322 58-59 0.032767892325037455 60-61 0.033091070443083626 62-63 0.03309783857121025 64-65 0.03280511702973388 66-67 0.029248465699194336 68-69 0.03061901164483517 70-71 0.0338575609534235 72-73 0.03733807084253855 74-75 0.03567480335542134 76-77 0.039635850341526516 78-79 0.042405706777346314 80-81 0.04267643190241117 82-83 0.0442178730832492 84-85 0.039947184235351096 86-87 0.03995733642754103 88-89 0.04473563488493573 90-91 0.04385070213237999 92-93 0.04809601049980325 94-95 0.051123055804434665 96-97 0.05066282309182441 98-99 0.04927535682586702 100-101 0.15285986577177574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.9550268E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.03044728633999 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8697965813613 11.10296083568809 2 9.537715527146258 2.8671226092575655 3 4.046025147025989 1.8244070307474043 4 2.3201306860360638 1.3949040789553957 5 1.5549970330601288 1.1686150467912677 6 1.1479001004465905 1.0352071169848118 7 0.8719525202960487 0.9174085474750744 8 0.6730904026340382 0.8093479852585816 9 0.5290577649739144 0.7156777363082346 >10 4.209780954514898 12.977283389139002 >50 0.6060337293995529 6.388973353715868 >100 0.514374750405104 15.642295519906495 >500 0.06157274017304119 6.430236859592056 >1k 0.04758432517550964 14.203987629365432 >5k 0.005288960915774596 5.467386454441634 >10k+ 0.004698776435902752 17.054185806373244 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT 154232 0.5219309686125351 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 104298 0.35295111367517884 No Hit ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA 102563 0.3470797625253348 No Hit ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG 91677 0.31024084113213457 No Hit TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC 86016 0.2910836544697327 No Hit CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT 82185 0.2781193050431895 No Hit CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA 78604 0.2660009716324739 No Hit CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA 73756 0.24959502905354364 No Hit AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG 68503 0.23181854052897252 No Hit CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG 65743 0.222478523714235 No Hit CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG 63846 0.21605895418613463 No Hit GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC 62264 0.2107053648379771 No Hit GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT 60711 0.20544991334765558 No Hit GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG 60215 0.2037714175722535 No Hit TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA 58792 0.1989558944101624 No Hit CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT 56982 0.19283073845557003 No Hit CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA 51785 0.17524375751854432 No Hit TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT 51604 0.1746312419230851 No Hit ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT 49476 0.16742995359635995 No Hit CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC 49136 0.1662793718148343 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 48773 0.16505095655985252 No Hit CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA 46674 0.15794780609096337 No Hit CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT 46060 0.1558699907560906 No Hit AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA 45064 0.15249946294903316 No Hit GTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTA 44936 0.15206630274892938 No Hit CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG 44757 0.15146055528159677 No Hit GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC 44544 0.1507397496361116 No Hit AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA 41501 0.14044204269145713 No Hit CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG 40890 0.13837437954877432 No Hit CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA 40243 0.13618489009981227 No Hit CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT 39695 0.13433042299311804 No Hit CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT 38419 0.1300123572483336 No Hit CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA 38408 0.12997513254363716 No Hit GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT 37583 0.12718327969140586 No Hit GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT 37335 0.1263440318037048 No Hit ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT 36979 0.12513930499716616 No Hit CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA 36203 0.12251327128403708 No Hit ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC 35576 0.12039146311634127 No Hit CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 34444 0.11656070259667356 No Hit TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA 34186 0.11568761406833943 No Hit CTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTGTGACTAGA 33787 0.11433737250707846 No Hit CTCGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAG 32799 0.1109939172125275 No Hit TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA 32688 0.1106182861015 No Hit CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT 31236 0.10570462508157287 No Hit CTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTC 31167 0.10547112466120442 No Hit CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT 30673 0.10379939701392896 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 30329 0.10263527897615007 No Hit TTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTG 30103 0.10187048049784184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.3840640633106948E-6 0.0 2 0.0 0.0 0.0 3.3840640633106948E-6 0.0 3 3.3840640633106948E-6 0.0 0.0 6.7681281266213895E-6 0.0 4 6.7681281266213895E-6 0.0 0.0 6.7681281266213895E-6 0.0 5 6.7681281266213895E-6 0.0 0.0 6.7681281266213895E-6 0.0 6 1.0152192189932084E-5 0.0 0.0 6.7681281266213895E-6 0.0 7 1.0152192189932084E-5 0.0 0.0 6.7681281266213895E-6 0.0 8 1.0152192189932084E-5 0.0 0.0 6.7681281266213895E-6 0.0 9 1.0152192189932084E-5 0.0 0.0 1.0152192189932084E-5 0.0 10-11 1.0152192189932084E-5 3.3840640633106948E-6 0.0 1.3536256253242779E-5 0.0 12-13 1.0152192189932084E-5 3.3840640633106948E-6 0.0 1.3536256253242779E-5 0.0 14-15 1.184422422158743E-5 3.3840640633106948E-6 0.0 1.6920320316553474E-5 0.0 16-17 2.368844844317486E-5 3.3840640633106948E-6 0.0 1.6920320316553474E-5 0.0 18-19 4.0608768759728335E-5 3.3840640633106948E-6 0.0 2.0304384379864168E-5 0.0 20-21 6.42972172029032E-5 3.3840640633106948E-6 0.0 2.0304384379864168E-5 0.0 22-23 8.121753751945667E-5 3.3840640633106948E-6 0.0 2.0304384379864168E-5 0.0 24-25 1.0321395393097619E-4 3.3840640633106948E-6 0.0 2.0304384379864168E-5 0.0 26-27 1.4043865862739383E-4 3.3840640633106948E-6 0.0 2.0304384379864168E-5 0.0 28-29 2.3011635630512727E-4 3.3840640633106948E-6 0.0 2.0304384379864168E-5 0.0 30-31 4.060876875972834E-4 3.3840640633106948E-6 0.0 2.3688448443174865E-5 0.0 32-33 7.867948947197365E-4 3.3840640633106948E-6 0.0 2.3688448443174865E-5 0.0 34-35 0.001412846746432215 3.3840640633106948E-6 0.0 2.7072512506485558E-5 0.0 36-37 0.0023925332927606613 3.3840640633106948E-6 0.0 2.7072512506485558E-5 0.0 38-39 0.004001655754864896 3.3840640633106948E-6 0.0 2.7072512506485558E-5 0.0 40-41 0.006422953592163699 3.3840640633106948E-6 0.0 2.7072512506485558E-5 0.0 42-43 0.00925879927721806 3.3840640633106948E-6 0.0 3.0456576569796255E-5 0.0 44-45 0.012363678055305624 3.3840640633106948E-6 0.0 3.384064063310695E-5 0.0 46-47 0.01627734814452444 3.3840640633106948E-6 0.0 3.722470469641764E-5 0.0 48-49 0.020724008323714696 3.3840640633106948E-6 0.0 3.722470469641764E-5 0.0 50-51 0.02605729328749235 3.3840640633106948E-6 0.0 3.722470469641764E-5 0.0 52-53 0.03264268195469496 3.3840640633106948E-6 0.0 4.0608768759728335E-5 0.0 54-55 0.041080845696560175 3.3840640633106948E-6 0.0 4.0608768759728335E-5 0.0 56-57 0.050957236665332445 3.3840640633106948E-6 0.0 4.0608768759728335E-5 0.0 58-59 0.062481666832937016 3.3840640633106948E-6 0.0 4.230080079138368E-5 0.0 60-61 0.07581826330644446 3.3840640633106948E-6 0.0 4.737689688634973E-5 0.0 62-63 0.09188072338294867 3.3840640633106948E-6 0.0 4.737689688634973E-5 0.0 64-65 0.11133401565089021 3.3840640633106948E-6 0.0 4.9068928918005076E-5 0.0 66-67 0.13447424571580874 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 68-69 0.16321340977347482 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 70-71 0.19694406832452416 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 72-73 0.2376357466538036 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 74-75 0.2897943260616114 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 76-77 0.35388511535665257 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 78-79 0.4279081326775107 3.3840640633106948E-6 0.0 5.076096094966042E-5 0.0 80-81 0.5170799127777792 3.3840640633106948E-6 0.0 5.245299298131577E-5 0.0 82-83 0.621798083184897 3.3840640633106948E-6 0.0 6.091315313959251E-5 0.0 84-85 0.7567427138055058 3.3840640633106948E-6 0.0 6.42972172029032E-5 0.0 86-87 0.912045873830992 3.3840640633106948E-6 0.0 6.42972172029032E-5 0.0 88-89 1.088052737795813 3.3840640633106948E-6 0.0 6.42972172029032E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 57075 0.0 87.75732 1 GCGTGTT 61655 0.0 80.405685 2 CGAACGT 10710 0.0 47.490486 1 CGTGTTT 111145 0.0 44.444866 3 CGCAGGA 16420 0.0 38.792156 1 TCGAACG 6680 0.0 35.61701 2 ACGTTTA 15410 0.0 34.484707 4 GAACGTT 15130 0.0 34.2438 2 CACGCGT 560 0.0 33.95348 1 GTAAACG 3715 0.0 33.779827 1 CGCGTTT 965 0.0 33.003483 1 TCGCAGG 10085 0.0 32.96236 2 GTCGAAC 7255 0.0 32.95664 1 CGCGTGA 405 0.0 31.689913 1 GTTTTTT 393995 0.0 31.671412 1 AACGTTT 16630 0.0 31.326298 3 GCAGGAT 20855 0.0 30.513409 2 CAGGATA 21135 0.0 30.198938 3 GTGTTTT 166670 0.0 29.179913 4 TAAACGC 4320 0.0 29.021265 2 >>END_MODULE