FastQCFastQC Report
Fri 27 May 2016
SRR2032145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29550268
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2801510.9480489314005546No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT725940.2456627466119766No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT723590.24486749155709858No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA675490.22859014341257414No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT653990.2213144056764561No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT628830.21280010049316642No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC625330.2116156780710077No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC573700.19414375531213457No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC544960.18441795519417964No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT507250.171656649611435No Hit
GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA472700.15996470827269654No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC455630.15418811091662518No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA408300.13817133570497567No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA407430.13787692213146763No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG393860.13328474719755504No Hit
GTCCAATGTACTGAGAGGCATGGGTGGTGCTTTTGTATTGGTATTGTATG389490.13180591120188825No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC388900.13160625142215293No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC384640.13016464013118256No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA380000.1285944344058064No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG371110.1255860014535232No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG360780.12209026327612325No Hit
CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT359930.12180261783074184No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG354510.11996845510842745No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT353930.11977217939275542No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA351290.1188787864800414No Hit
CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA345000.11675021018421898No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA339750.11497357655098085No Hit
CCCAAATAAATCTGTACTCAGAAAAAGCAAATCCATATGAATGCGGATTC333060.11270963769262601No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC330870.11196852766276096No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA330630.11188731012524152No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG318460.10776890416019239No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT311980.10557603064716706No Hit
CTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCAATTACAT305640.10343053403102807No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG302240.10227995224950245No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG301900.10216489407134989No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA300860.10181295140876556No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT299200.101251196774256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA130700.053.160181
CCGAAAC140150.048.2963872
CCAAACG142050.046.8109978
TCCGCTC28200.045.004912
CTCCGCT37450.040.127961
GTCCGTA17650.038.2609251
ACTCGTC105150.037.76265342-43
CTAACGA102300.037.4914394-95
CGCCTAT12300.036.730791
TACGCAT108350.034.5428669
TCGTCTA118100.033.60156244-45
CCGCTCA34050.033.3595123
CTACGCA112850.033.123358
CGAGCTA127200.031.68312512-13
CTCTACG117800.031.4930926
CGACCGT3100.030.681751
CAACTCG132750.030.0761840-41
CGCCCAT16250.029.850981
TCCACTC950100.029.6676482
CGAAACC227200.029.2231483
Produced by FastQC (version 0.11.5)