FastQCFastQC Report
Fri 27 May 2016
SRR2032144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31512485
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1576370.5002366522348206No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC1416800.4495995793413309No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC1137490.36096486836883857No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1046780.332179452048926No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA1037420.3292092007342487No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC946350.3003095439791562No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG862230.2736153622921201No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT822900.26113459474871625No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG814490.25846581124909696No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA810450.2571837796987448No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA808310.25650468377850877No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC749300.2377787724452705No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT724970.2300580230343624No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT720890.22876329810232357No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT698760.22174068468418154No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA680680.21600327616181333No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG674730.21411513563592333No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA656410.2083015668234352No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA592970.18816986346839992No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT568760.1804871942025518No Hit
AGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG547370.17369940834561287No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG539140.1710877450635835No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC531720.16873312276070895No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG527610.16742887779240515No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA521470.16548044370350354No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG479660.1522126864955271No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA465030.1475700821436329No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA459650.14586282230677777No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA459100.14568828830858627No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT458820.14559943463677968No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA454130.14411113563401934No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT433480.13755817733828354No Hit
ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT419870.13323925421939908No Hit
CAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACC415240.13176999528916872No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA399750.12685448323101145No Hit
ATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTA387580.12299252185284656No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA380640.12079022013021189No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT379780.1205173124239488No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC374710.11890842629516524No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT363170.11524638567856518No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA362070.11489731768218214No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT353660.11222853418256287No Hit
CTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAATTAGCAAGAG346510.10995959220607325No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG343020.10885209465391257No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG338600.10744947597753715No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT326490.10360655467190226No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC325700.10335586038359083No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG323910.102787831553113No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC320630.10174697425480725No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA315770.10020472837987864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT851350.089.834191
GCGTGTT929550.081.890192
GTAAACG274000.053.1303021
TAAACGC293650.049.4737432
CGTGTTT1593700.047.7697453
TCGAACG49400.043.9353942
CGAACGT91500.041.441121
CGCGTTT13200.040.057931
GTCGAAC58500.037.620591
CGCAGGA130750.036.724711
AAACGCT420250.034.988013
GTGTTTT2213700.034.1231044
AGTAAAC176850.033.1583181
ACGCTTC452850.032.3129355
AACGCTT470150.031.2245464
GTTTTTT5326350.030.51271
CGCTTCG484950.030.0449376
GAACGTT127950.029.9913752
CACGCGT6950.029.4729181
TCGCAGG85750.029.1878282
Produced by FastQC (version 0.11.5)