##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032144_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31512485
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82019954948015	33.0	31.0	34.0	30.0	34.0
2	31.891133688758597	34.0	31.0	34.0	30.0	34.0
3	32.19829194682679	34.0	31.0	34.0	30.0	34.0
4	35.57566259849072	37.0	35.0	37.0	33.0	37.0
5	35.71987096542846	37.0	35.0	37.0	35.0	37.0
6	35.727020449196566	37.0	35.0	37.0	35.0	37.0
7	35.82658247992819	37.0	35.0	37.0	35.0	37.0
8	35.83681312343346	37.0	35.0	37.0	35.0	37.0
9	37.60397245726575	39.0	38.0	39.0	35.0	39.0
10-11	37.47508025787239	39.0	37.5	39.0	35.0	39.0
12-13	37.41204771696044	39.0	37.0	39.0	34.5	39.0
14-15	38.66650416493653	40.0	38.0	41.0	34.0	41.0
16-17	38.47369280778714	40.0	38.0	41.0	34.0	41.0
18-19	38.16738443508977	40.0	38.0	41.0	33.0	41.0
20-21	37.78132413232406	40.0	38.0	41.0	32.5	41.0
22-23	37.45478958577846	40.0	38.0	41.0	32.0	41.0
24-25	37.26749743792024	40.0	38.0	41.0	31.5	41.0
26-27	36.97240696822227	40.0	38.0	41.0	30.5	41.0
28-29	36.66833207536632	40.0	37.0	41.0	29.5	41.0
30-31	36.4269190925438	40.0	37.0	41.0	28.5	41.0
32-33	36.263859609929206	40.0	37.0	41.0	28.0	41.0
34-35	36.07078108882877	40.0	37.0	41.0	27.0	41.0
36-37	35.941018567720064	40.0	37.0	41.0	26.0	41.0
38-39	35.83532503069815	40.0	36.0	41.0	26.0	41.0
40-41	35.70869353844992	40.0	36.0	41.0	25.5	41.0
42-43	35.64283375303471	40.0	36.0	41.0	25.5	41.0
44-45	35.69533952971338	40.0	36.0	41.0	25.5	41.0
46-47	35.576995498768184	40.0	36.0	41.0	24.5	41.0
48-49	35.43352729878332	40.0	35.0	41.0	24.0	41.0
50-51	35.357861241346086	40.0	35.0	41.0	24.0	41.0
52-53	35.20362719728387	40.0	35.0	41.0	23.0	41.0
54-55	35.0411516895605	40.0	35.0	41.0	22.5	41.0
56-57	34.90466786418145	39.0	35.0	41.0	22.5	41.0
58-59	34.74197799697485	39.0	35.0	41.0	22.0	41.0
60-61	34.66248245734984	39.0	35.0	41.0	22.0	41.0
62-63	34.688925357679665	39.0	35.0	41.0	22.5	41.0
64-65	34.478658839504405	39.0	34.5	41.0	22.0	41.0
66-67	34.212857062843504	38.5	34.0	41.0	21.0	41.0
68-69	33.92756229792731	37.5	34.0	40.0	20.0	41.0
70-71	33.560723567182976	37.0	34.0	40.0	19.0	41.0
72-73	33.17882742347993	36.5	34.0	39.0	17.5	41.0
74-75	32.79480087019478	36.0	33.5	39.0	16.0	41.0
76-77	32.482142823709395	36.0	33.0	39.0	15.5	40.5
78-79	32.13466854486404	35.0	33.0	37.5	11.5	39.5
80-81	31.77240828516063	35.0	33.0	37.0	7.5	39.0
82-83	31.459323788650753	35.0	33.0	37.0	7.0	39.0
84-85	31.160981655366122	35.0	33.0	36.0	2.0	37.5
86-87	30.86548593359108	35.0	33.0	36.0	2.0	37.0
88-89	30.60823403803286	35.0	32.5	35.5	2.0	37.0
90-91	30.386252845499172	35.0	32.0	35.0	2.0	36.0
92-93	30.190610705566378	35.0	32.0	35.0	2.0	36.0
94-95	29.98555250403134	35.0	32.0	35.0	2.0	36.0
96-97	29.83375754086039	35.0	32.0	35.0	2.0	36.0
98-99	29.638661597141578	35.0	32.0	35.0	2.0	35.5
100-101	28.949314977857192	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40198203552997924
1101	2	0.4479361586168231
1101	3	0.2523324547694443
1101	4	0.33344202765621134
1101	5	0.21754443011070634
1101	6	0.19913801083619376
1101	7	0.1720327691725103
1101	8	0.17083203241958245
1101	9	0.21938239066814447
1101	10-11	0.2661189876459602
1101	12-13	0.20811128883394048
1101	14-15	0.2712043009754481
1101	16-17	0.2527275543857215
1101	18-19	0.2544470431781889
1101	20-21	0.23671988932952104
1101	22-23	0.21136161138303322
1101	24-25	0.05225964426578855
1101	26-27	0.025227085589094145
1101	28-29	-0.05108392898936387
1101	30-31	0.009608925972045768
1101	32-33	-0.026146038632120394
1101	34-35	-0.10189095572343376
1101	36-37	-0.06641348051519458
1101	38-39	-0.056049849403351004
1101	40-41	-0.07992784210276227
1101	42-43	-0.11532436416160152
1101	44-45	-0.15589601528194663
1101	46-47	-0.16426494429410354
1101	48-49	-0.13312649764858264
1101	50-51	-0.14176077774597928
1101	52-53	-0.17871585441293547
1101	54-55	-0.15181223519539344
1101	56-57	-0.13958105934887755
1101	58-59	-0.13001042874715552
1101	60-61	-0.11766034666517555
1101	62-63	-0.12482481316035887
1101	64-65	-0.11717652826195035
1101	66-67	-0.13157364924276038
1101	68-69	-0.08390692920836074
1101	70-71	-0.10599148991157392
1101	72-73	-0.13133029894875392
1101	74-75	-0.14360859821015737
1101	76-77	-0.1656046715574533
1101	78-79	-0.14904033210621037
1101	80-81	-0.14232619342663355
1101	82-83	-0.16717719564233846
1101	84-85	-0.17592132401200544
1101	86-87	-0.17944843236479358
1101	88-89	-0.13992185011559855
1101	90-91	-0.1733123025858596
1101	92-93	-0.15106335142103333
1101	94-95	-0.12614620110879216
1101	96-97	-0.12295735464026336
1101	98-99	-0.11323227970048322
1101	100-101	0.021590828507100923
1102	1	0.32838461820164255
1102	2	0.3413355129489055
1102	3	0.22811990787126746
1102	4	0.2882888996569122
1102	5	0.1782372143354678
1102	6	0.15987442127158147
1102	7	0.13534312603491117
1102	8	0.12349759388891357
1102	9	0.16205754990381394
1102	10-11	0.2017048876546852
1102	12-13	0.1749226490991873
1102	14-15	0.23338037636153786
1102	16-17	0.2053276765391132
1102	18-19	0.21176970263199024
1102	20-21	0.1484858783357197
1102	22-23	0.12176428130712935
1102	24-25	0.05871632343863098
1102	26-27	0.0062605905188561906
1102	28-29	-0.03438599687174815
1102	30-31	-0.052318480013468616
1102	32-33	-0.08259835518390446
1102	34-35	-0.108958401845058
1102	36-37	-0.1440681354318727
1102	38-39	-0.14066288491508772
1102	40-41	-0.1038895382898346
1102	42-43	-0.1379772740298577
1102	44-45	-0.17977045904776645
1102	46-47	-0.16813677048728692
1102	48-49	-0.19025502046509501
1102	50-51	-0.20055800311898508
1102	52-53	-0.1859250857191057
1102	54-55	-0.17535948238150212
1102	56-57	-0.2174211256607208
1102	58-59	-0.17374570490106578
1102	60-61	-0.1469444405487792
1102	62-63	-0.11763739495950176
1102	64-65	-0.14135635550215397
1102	66-67	-0.1388374133122099
1102	68-69	-0.1456052975880766
1102	70-71	-0.20358332308494198
1102	72-73	-0.24361325855088722
1102	74-75	-0.21177455231537579
1102	76-77	-0.21010340639734437
1102	78-79	-0.20959351245696567
1102	80-81	-0.25109724307326076
1102	82-83	-0.2818159706183465
1102	84-85	-0.2622030620802356
1102	86-87	-0.25520534711917975
1102	88-89	-0.2328674918571565
1102	90-91	-0.23594863449406844
1102	92-93	-0.20619597510134113
1102	94-95	-0.21752129926776576
1102	96-97	-0.18072936406352724
1102	98-99	-0.2062760804159538
1102	100-101	-0.11678060505018095
1103	1	0.31076944533873174
1103	2	0.3442489498015995
1103	3	0.19234733557718187
1103	4	0.26814620115269605
1103	5	0.12842824048505008
1103	6	0.15042584107810342
1103	7	0.12283417963491416
1103	8	0.11330082237470407
1103	9	0.10170371051534488
1103	10-11	0.22631233913710247
1103	12-13	0.17050358018969547
1103	14-15	0.2184921414895129
1103	16-17	0.20235796126554106
1103	18-19	0.20263653916084934
1103	20-21	0.16018811533634647
1103	22-23	0.13077098359290318
1103	24-25	0.08865673603525437
1103	26-27	0.01935756367917918
1103	28-29	-0.01178582604173073
1103	30-31	-0.00897163313919691
1103	32-33	-0.03664378426476844
1103	34-35	-0.07441926276067079
1103	36-37	-0.058576335981101124
1103	38-39	-0.06568783915706433
1103	40-41	-0.03463962171684898
1103	42-43	-0.07380017323268362
1103	44-45	-0.1446956362796996
1103	46-47	-0.1173982491958796
1103	48-49	-0.08784454173675016
1103	50-51	-0.13558354418365326
1103	52-53	-0.12172339247082675
1103	54-55	-0.14078698162929726
1103	56-57	-0.11501240686743586
1103	58-59	-0.07960989303700927
1103	60-61	-0.08235035888085207
1103	62-63	-0.08340172093462428
1103	64-65	-0.0672339392662451
1103	66-67	-0.055172743887233366
1103	68-69	-0.043579395128503506
1103	70-71	-0.046450574901371056
1103	72-73	-0.10959078490548535
1103	74-75	-0.10645354410137742
1103	76-77	-0.09838152865472694
1103	78-79	-0.09104221290854753
1103	80-81	-0.11732873928834309
1103	82-83	-0.12726428570030635
1103	84-85	-0.1491882164940428
1103	86-87	-0.13282846893009648
1103	88-89	-0.11196021655138111
1103	90-91	-0.16808532401841703
1103	92-93	-0.13961215264216875
1103	94-95	-0.12982098548685883
1103	96-97	-0.13738823150516666
1103	98-99	-0.1327554301413265
1103	100-101	-0.00995575612485311
1104	1	0.2275384412467325
1104	2	0.3021079994699001
1104	3	0.16257623806098565
1104	4	0.2273690212309063
1104	5	0.12319546974400453
1104	6	0.1295236791493366
1104	7	0.08569041272688338
1104	8	0.04120762066867201
1104	9	0.06269122874407884
1104	10-11	0.10831759678672626
1104	12-13	0.11729739040108456
1104	14-15	0.12212260924093954
1104	16-17	0.10294292230644686
1104	18-19	0.10485160703869667
1104	20-21	0.016469415114826802
1104	22-23	0.022089920261663565
1104	24-25	0.005710034001012332
1104	26-27	0.00805415223079109
1104	28-29	-0.0443270473635522
1104	30-31	-0.01060989612732044
1104	32-33	-0.09599572869505124
1104	34-35	-0.10106546487840262
1104	36-37	-0.14557574795197326
1104	38-39	-0.11003742207108758
1104	40-41	-0.1154744707466051
1104	42-43	-0.18515040159112317
1104	44-45	-0.1811282486411372
1104	46-47	-0.14750634709972843
1104	48-49	-0.12854537786414966
1104	50-51	-0.16372606347440666
1104	52-53	-0.19153239597648763
1104	54-55	-0.21576101232916045
1104	56-57	-0.2059933633386919
1104	58-59	-0.18189070296936194
1104	60-61	-0.16630445263914595
1104	62-63	-0.12891464666521557
1104	64-65	-0.1685443540456788
1104	66-67	-0.17771734036995923
1104	68-69	-0.1706321623315148
1104	70-71	-0.17189646342835374
1104	72-73	-0.1729144809539136
1104	74-75	-0.22786751223480195
1104	76-77	-0.18783382834759976
1104	78-79	-0.1956065609428741
1104	80-81	-0.18004380659097663
1104	82-83	-0.21668549153386607
1104	84-85	-0.2150105592624989
1104	86-87	-0.22281734208003456
1104	88-89	-0.16852044953986223
1104	90-91	-0.15929787146131602
1104	92-93	-0.1744684469392972
1104	94-95	-0.15376914208681924
1104	96-97	-0.1478707548722049
1104	98-99	-0.1752723745614162
1104	100-101	-0.080341366490714
1105	1	0.21131719510087166
1105	2	0.27878778111002234
1105	3	0.12166878716675456
1105	4	0.23398017401904525
1105	5	0.1238793317636464
1105	6	0.13487665788466074
1105	7	0.09760211118635453
1105	8	0.11655953463640856
1105	9	0.11158434082228297
1105	10-11	0.17739684146015833
1105	12-13	0.11820408663837867
1105	14-15	0.17732525019817302
1105	16-17	0.1404879304215214
1105	18-19	0.15404991826235914
1105	20-21	0.19378190319447697
1105	22-23	0.14578847054352906
1105	24-25	0.11531147029936051
1105	26-27	0.0489289169842948
1105	28-29	-0.005311817395110552
1105	30-31	0.02324002209197573
1105	32-33	-0.002748497314350118
1105	34-35	-0.021248678911440777
1105	36-37	0.003947969927601491
1105	38-39	0.02683283550671689
1105	40-41	0.055136582355203245
1105	42-43	-0.013952766194542221
1105	44-45	-0.05869635402522988
1105	46-47	-0.10108161724067344
1105	48-49	-0.0074454369450691615
1105	50-51	-0.04570336598461466
1105	52-53	0.003664830206830061
1105	54-55	-0.02648339711425507
1105	56-57	-0.04838185187674071
1105	58-59	-0.06369465508279859
1105	60-61	-0.014923497073198178
1105	62-63	-0.01148794261677466
1105	64-65	-0.04323300978762035
1105	66-67	-0.04291500701809525
1105	68-69	-0.0410157655836656
1105	70-71	0.013168618577623192
1105	72-73	0.008721240971929944
1105	74-75	0.008425305351615009
1105	76-77	0.022574503118327982
1105	78-79	0.006138167520440163
1105	80-81	0.008164566152398578
1105	82-83	-0.009151180310222884
1105	84-85	-0.03456792980611212
1105	86-87	-0.05729000562416431
1105	88-89	-0.05811763774199363
1105	90-91	-0.05828804894166595
1105	92-93	-0.030255962847832052
1105	94-95	-0.024294973293933708
1105	96-97	-0.0662260688444114
1105	98-99	-0.02923032444051543
1105	100-101	0.06765340688545507
1106	1	0.21531064976018044
1106	2	0.26160837122277414
1106	3	0.13838037735781938
1106	4	0.11484621712914134
1106	5	0.0811042557240853
1106	6	-0.02449205598026083
1106	7	0.01190589118012042
1106	8	0.01949228413015902
1106	9	0.041898596132753596
1106	10-11	0.10798364700572449
1106	12-13	0.08302437517171057
1106	14-15	0.07860205095965256
1106	16-17	0.033615074030514336
1106	18-19	0.06671550510296242
1106	20-21	0.038509643111538594
1106	22-23	-0.034625857222557954
1106	24-25	-0.06546591784909594
1106	26-27	-0.04317086450893015
1106	28-29	-0.07421712684971737
1106	30-31	-0.07673541346243695
1106	32-33	-0.14913701671055435
1106	34-35	-0.09740144975826581
1106	36-37	-0.08999889470673139
1106	38-39	-0.09441409117856381
1106	40-41	-0.0704326753417277
1106	42-43	-0.10866050838419739
1106	44-45	-0.11295426064329206
1106	46-47	-0.12958893294439378
1106	48-49	-0.12384282742534225
1106	50-51	-0.11335599209063929
1106	52-53	-0.1951626198458598
1106	54-55	-0.1749219105774884
1106	56-57	-0.1272642182173911
1106	58-59	-0.16565803098080778
1106	60-61	-0.1313226477446463
1106	62-63	-0.14447797068680046
1106	64-65	-0.09535893375177551
1106	66-67	-0.07748687977237267
1106	68-69	-0.10899036697510667
1106	70-71	-0.074666345451746
1106	72-73	-0.05461893075064239
1106	74-75	-0.09383128207301894
1106	76-77	-0.08610477040998177
1106	78-79	-0.09560042079004205
1106	80-81	-0.08976373913806057
1106	82-83	-0.11356222775053126
1106	84-85	-0.126924950665245
1106	86-87	-0.09587185927701114
1106	88-89	-0.07160253210272316
1106	90-91	-0.112552325775372
1106	92-93	-0.11261075329329984
1106	94-95	-0.074795668052559
1106	96-97	-0.08556597368847818
1106	98-99	-0.06253275012084458
1106	100-101	0.08076403710737523
1107	1	0.1520794473404372
1107	2	0.24282727467431542
1107	3	0.09649976491186152
1107	4	0.17699353367861193
1107	5	0.020320024420968252
1107	6	0.08081256245534263
1107	7	0.03567152537906537
1107	8	0.05088948614130828
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2216	44-45	-0.5197344783085285
2216	46-47	-0.41236354711784173
2216	48-49	-0.41946572177449326
2216	50-51	-0.518134203556393
2216	52-53	-0.6379446478591717
2216	54-55	-0.5509134248382352
2216	56-57	-0.6553936504030204
2216	58-59	-0.6170631836448308
2216	60-61	-0.6476155537333952
2216	62-63	-0.5219929256619693
2216	64-65	-0.6520656834289937
2216	66-67	-0.8065151997781825
2216	68-69	-0.5763490853767479
2216	70-71	-0.5552084258556818
2216	72-73	-0.5117506204692646
2216	74-75	-0.534774615678451
2216	76-77	-0.4991063664741091
2216	78-79	-0.494273309472149
2216	80-81	-0.43590554394195635
2216	82-83	-0.4905495815647072
2216	84-85	-0.46881212800317584
2216	86-87	-0.5009747922348033
2216	88-89	-0.540513754264925
2216	90-91	-0.46440884811351424
2216	92-93	-0.4555695333255443
2216	94-95	-0.5095311757787897
2216	96-97	-0.5399513825178026
2216	98-99	-0.519705425974724
2216	100-101	-0.3693802764077816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91230.0
3	27969.0
4	35823.0
5	62452.0
6	187457.0
7	202265.0
8	168591.0
9	209783.0
10	292362.0
11	307116.0
12	239187.0
13	159282.0
14	114894.0
15	98648.0
16	91311.0
17	90891.0
18	87984.0
19	90555.0
20	98133.0
21	108079.0
22	128487.0
23	161648.0
24	201800.0
25	244582.0
26	283376.0
27	320861.0
28	356033.0
29	398096.0
30	435935.0
31	508702.0
32	614972.0
33	759322.0
34	1031638.0
35	1541849.0
36	2673499.0
37	5845016.0
38	9422461.0
39	3810042.0
40	10154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.641841426962785	18.02750790807635	14.655969394433102	36.67468127052776
2	20.828864577928414	20.75719626342059	48.71649472968565	9.69744442896535
3	14.366272606706321	22.2627561596073	45.32220290120059	18.04876833248579
4	20.212086936804308	28.321851563971855	38.071020203778254	13.395041295445578
5	18.733579615358497	31.241306551721372	38.96238550712942	11.062728325790717
6	17.347808858670074	33.05625310979264	37.070448682374746	12.525489349162536
7	14.52476634570691	17.04283231421765	55.72727100749301	12.70513033258243
8	14.562849315534624	20.628368567719374	48.01151957830279	16.79726253844322
9	16.38976540825717	21.465659805326414	46.172138017145855	15.972436769270566
10-11	17.81611658250545	28.723782271666554	38.97211737913725	14.487983766690748
12-13	18.47386856784262	22.358759665072434	43.204486762888	15.962885004196945
14-15	19.095364827354583	24.561415932755757	41.80707693273086	14.5361423071588
16-17	19.661892116900294	25.314210475617116	39.66020971960193	15.363687687880661
18-19	20.42142969005552	26.425287495913157	36.61368593180394	16.539596882227382
20-21	21.343006749613565	26.36133368840848	36.158846869017374	16.136812692960582
22-23	21.793178961135297	27.846595483731583	34.26763614549536	16.09258940963776
24-25	21.03467288182461	28.296201802167566	33.11530938588211	17.55381593012571
26-27	21.09675475255027	28.692231043884362	32.732986991743644	17.478027211821722
28-29	22.02417771120364	28.443019943336555	32.35532124637225	17.177481099087554
30-31	21.679121807218444	30.399789810325252	30.319077927742537	17.60201045471377
32-33	21.463870009405053	29.078038766414487	31.186511023490088	18.27158020069037
34-35	22.193374634451665	29.536542020520066	30.154799728009667	18.115283617018598
36-37	22.65044185620087	29.85364808195593	29.95167324311259	17.544236818730607
38-39	22.975302958667633	27.744409957199025	31.102544781531105	18.177742302602233
40-41	22.840942977547403	29.251056603892806	29.713691863249448	18.19430855531034
42-43	23.335337989946662	28.999100642836783	29.786355249333695	17.879206117882855
44-45	23.63017376642949	28.65121718100877	29.611218539954308	18.107390512607434
46-47	22.466269665511287	28.587134355871658	29.589184449010087	19.35741152960697
48-49	22.67501944865137	28.755040585011372	30.212689384655594	18.35725058168167
50-51	22.118243635921576	28.676152375373036	30.33978294598649	18.8658210427189
52-53	23.570859182632915	28.087410222046298	28.906490530168753	19.43524006515203
54-55	22.94269832550792	28.105022256848006	29.964776900256712	18.98750251738736
56-57	23.528253043102882	28.039289597422535	29.87763578627774	18.554821573196843
58-59	23.31903336334418	27.992956393811802	29.594969150251977	19.093041092592042
60-61	23.20107805460752	27.983384785954037	28.91096554181819	19.90457161762026
62-63	23.491412340409063	28.021368761942345	29.109306407583713	19.377912490064883
64-65	23.716943697835397	28.327547470397104	28.762803241861263	19.19270558990624
66-67	23.31397607888658	28.198993047710424	28.739127865205933	19.747903008197063
68-69	24.363115040967294	27.359011017555023	28.93159586875856	19.346278072719123
70-71	23.307914394862138	27.43409465057421	29.124591214880606	20.133399739683043
72-73	23.490621498831164	27.232122773360203	29.121552660801804	20.15570306700684
74-75	24.535047875430063	26.741022791330373	29.566777243879507	19.15715208936006
76-77	23.470941882240563	27.319219749706363	29.182944078459915	20.026894289593155
78-79	23.053738420343272	27.49400756246712	29.378674843407378	20.07357917378223
80-81	22.390365028376014	26.798082676671797	30.395816265413742	20.415736029538444
82-83	22.28636908815495	27.897957300046688	29.076695113355953	20.738978498442407
84-85	21.840157622335383	27.942345313670614	29.209300533029513	21.008196530964486
86-87	22.426557577768342	28.71788484147948	28.9100245772529	19.945533003499275
88-89	22.807859244175546	26.544351580898883	30.697258616458623	19.95053055846694
90-91	23.472927678671116	27.04623809917928	29.1844804862606	20.29635373588901
92-93	22.69028537449295	26.90677577366501	30.119672826299297	20.28326602554274
94-95	23.55255036344657	27.208042470532014	28.920119524157105	20.31928764186431
96-97	23.061369083296714	27.496140690510106	29.174860967732887	20.267629258460296
98-99	24.3845379166993	26.28135330168216	29.30070387882057	20.03340490279797
100-101	24.60050385358661	26.744332734252772	28.1053068374328	20.54985657472782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1620.0
1	2613.0
2	2653.0
3	1539.0
4	1338.5
5	1514.5
6	2050.5
7	2753.0
8	3750.0
9	5050.5
10	6609.0
11	8648.0
12	11225.0
13	14419.0
14	18309.5
15	23058.5
16	28772.0
17	35788.0
18	45615.0
19	56544.5
20	76109.5
21	101344.5
22	126390.0
23	155463.5
24	193197.0
25	248130.0
26	310646.5
27	438926.0
28	585280.5
29	734591.0
30	928539.0
31	1002857.5
32	1086300.0
33	1251953.0
34	1498867.0
35	1493399.0
36	1325980.0
37	1554209.5
38	1612757.5
39	1475492.0
40	1419559.5
41	1270931.5
42	1072224.5
43	896761.0
44	822161.0
45	765703.5
46	669022.5
47	612607.0
48	614228.0
49	607187.0
50	550293.0
51	511354.0
52	528672.5
53	522025.0
54	548067.5
55	499711.5
56	392013.0
57	386698.5
58	355865.0
59	273144.5
60	208666.0
61	158837.5
62	118858.5
63	87456.5
64	67699.0
65	60481.5
66	51126.5
67	34463.0
68	20191.5
69	14722.5
70	13045.0
71	25096.0
72	65708.5
73	128726.5
74	159497.5
75	125089.5
76	81503.0
77	103706.5
78	110847.0
79	56130.0
80	19148.0
81	5748.5
82	1736.5
83	683.5
84	353.0
85	219.0
86	165.5
87	134.0
88	121.5
89	107.0
90	86.5
91	72.0
92	66.0
93	59.0
94	41.0
95	28.5
96	22.5
97	19.0
98	15.0
99	19.0
100	38.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3062310065359809
2	0.024206278876451666
3	0.030670383500380878
4	0.04857439836940819
5	0.0441348881245005
6	0.02274971332790797
7	0.023460542702360667
8	0.027144796736912367
9	0.02784927942052174
10-11	0.031260625748810354
12-13	0.05760573943946344
14-15	0.07216504823405707
16-17	0.050021443881686896
18-19	0.05268546736317368
20-21	0.0592844391675236
22-23	0.0526569072543787
24-25	0.07849587235027641
26-27	0.050859207073006146
28-29	0.05745659220464524
30-31	0.05433560698243886
32-33	0.06802859247691828
34-35	0.06640066627560473
36-37	0.05556527833333359
38-39	0.04968030924885804
40-41	0.05958432030987083
42-43	0.047560514507186596
44-45	0.05546214460712953
46-47	0.05190006437131188
48-49	0.04492505113449479
50-51	0.048704505531696404
52-53	0.05174457044565035
54-55	0.06823644660203726
56-57	0.05499090281201244
58-59	0.04754623445278911
60-61	0.05704564397253977
62-63	0.053578764099372046
64-65	0.05315670915829075
66-67	0.04811902330139943
68-69	0.047535127743813285
70-71	0.049177333999524314
72-73	0.04749863427146415
74-75	0.049631122394822244
76-77	0.046713231279602356
78-79	0.04453472964763014
80-81	0.03843555974719227
82-83	0.03542246826932246
84-85	0.04009363273001161
86-87	0.029737419946411714
88-89	0.02790639963811169
90-91	0.024014291478441006
92-93	0.03468783880420729
94-95	0.029289978241957115
96-97	0.021734242792975547
98-99	0.025791364914572748
100-101	0.021278867724966788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1512485E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.254575961381306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58532663986401	13.559564879574953
2	8.077732247618389	2.7875568932446164
3	3.308079986076276	1.7123855221823494
4	1.9346480325340063	1.3352612574357963
5	1.2469284805721637	1.0757611093221084
6	0.893658825980751	0.9251822457866222
7	0.6798815047283977	0.8211746947652051
8	0.520845628496025	0.7189576368830439
9	0.42112524928308487	0.6539703842709563
>10	3.3761737701004226	11.785701685497004
>50	0.464172385834135	5.61010923463396
>100	0.38901040064953174	13.729034153974567
>500	0.05218442960729051	6.274303811474742
>1k	0.04126246486366135	14.221443422722539
>5k	0.004744438369483375	5.6640622461960355
>10k+	0.0042255154228211305	19.125530822035156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	157637	0.5002366522348206	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	141680	0.4495995793413309	No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC	113749	0.36096486836883857	No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	104678	0.332179452048926	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	103742	0.3292092007342487	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	94635	0.3003095439791562	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	86223	0.2736153622921201	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	82290	0.26113459474871625	No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG	81449	0.25846581124909696	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	81045	0.2571837796987448	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	80831	0.25650468377850877	No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC	74930	0.2377787724452705	No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT	72497	0.2300580230343624	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	72089	0.22876329810232357	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	69876	0.22174068468418154	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	68068	0.21600327616181333	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	67473	0.21411513563592333	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	65641	0.2083015668234352	No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA	59297	0.18816986346839992	No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT	56876	0.1804871942025518	No Hit
AGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG	54737	0.17369940834561287	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	53914	0.1710877450635835	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	53172	0.16873312276070895	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	52761	0.16742887779240515	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	52147	0.16548044370350354	No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG	47966	0.1522126864955271	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	46503	0.1475700821436329	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	45965	0.14586282230677777	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	45910	0.14568828830858627	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	45882	0.14559943463677968	No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA	45413	0.14411113563401934	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	43348	0.13755817733828354	No Hit
ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT	41987	0.13323925421939908	No Hit
CAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACC	41524	0.13176999528916872	No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA	39975	0.12685448323101145	No Hit
ATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTA	38758	0.12299252185284656	No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA	38064	0.12079022013021189	No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT	37978	0.1205173124239488	No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC	37471	0.11890842629516524	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	36317	0.11524638567856518	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	36207	0.11489731768218214	No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT	35366	0.11222853418256287	No Hit
CTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAATTAGCAAGAG	34651	0.10995959220607325	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	34302	0.10885209465391257	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	33860	0.10744947597753715	No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT	32649	0.10360655467190226	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	32570	0.10335586038359083	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	32391	0.102787831553113	No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC	32063	0.10174697425480725	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	31577	0.10020472837987864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.173345421663826E-6	0.0	0.0	3.173345421663826E-6	0.0
2	3.173345421663826E-6	3.173345421663826E-6	0.0	3.173345421663826E-6	0.0
3	3.173345421663826E-6	3.173345421663826E-6	0.0	3.173345421663826E-6	0.0
4	9.520036264991478E-6	3.173345421663826E-6	0.0	3.173345421663826E-6	0.0
5	9.520036264991478E-6	3.173345421663826E-6	0.0	3.173345421663826E-6	0.0
6	1.2693381686655305E-5	3.173345421663826E-6	0.0	3.173345421663826E-6	0.0
7	1.2693381686655305E-5	3.173345421663826E-6	0.0	3.173345421663826E-6	0.0
8	1.2693381686655305E-5	3.173345421663826E-6	0.0	6.346690843327652E-6	0.0
9	1.2693381686655305E-5	3.173345421663826E-6	0.0	9.520036264991478E-6	0.0
10-11	1.4280054397487219E-5	3.173345421663826E-6	0.0	9.520036264991478E-6	0.0
12-13	1.9040072529982956E-5	3.173345421663826E-6	0.0	1.110670897582339E-5	0.0
14-15	2.3800090662478697E-5	4.760018132495739E-6	0.0	1.2693381686655305E-5	0.0
16-17	3.808014505996591E-5	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
18-19	5.7120217589948875E-5	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
20-21	9.202701722825096E-5	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
22-23	1.697739800590147E-4	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
24-25	3.395479601180294E-4	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
26-27	5.585087942128334E-4	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
28-29	8.330031731867543E-4	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
30-31	0.001242364732581388	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
32-33	0.0019785808704073956	6.346690843327652E-6	0.0	1.2693381686655305E-5	0.0
34-35	0.0032098388940129605	7.933363554159566E-6	0.0	1.4280054397487219E-5	0.0
36-37	0.004966285584903888	9.520036264991478E-6	0.0	1.9040072529982956E-5	0.0
38-39	0.007877830009280449	9.520036264991478E-6	0.0	2.221341795164678E-5	0.0
40-41	0.011846098459071063	9.520036264991478E-6	0.0	2.538676337331061E-5	0.0
42-43	0.016441102629640283	9.520036264991478E-6	0.0	2.6973436084142525E-5	0.0
44-45	0.022034123935322777	9.520036264991478E-6	0.0	2.8560108794974438E-5	0.0
46-47	0.02867910924828683	9.520036264991478E-6	0.0	2.8560108794974438E-5	0.0
48-49	0.036377645241243275	9.520036264991478E-6	0.0	2.8560108794974438E-5	0.0
50-51	0.04490918440738647	9.520036264991478E-6	0.0	2.8560108794974438E-5	0.0
52-53	0.05477352865062847	9.520036264991478E-6	0.0	3.014678150580635E-5	0.0
54-55	0.06659424034632623	9.520036264991478E-6	0.0	3.3320126927470175E-5	0.0
56-57	0.08033799936755226	9.520036264991478E-6	0.0	3.490679963830209E-5	0.0
58-59	0.09621583318484721	9.520036264991478E-6	0.0	3.490679963830209E-5	0.0
60-61	0.1139945009097188	9.520036264991478E-6	0.0	3.490679963830209E-5	0.0
62-63	0.13502108767366333	1.110670897582339E-5	0.0	3.490679963830209E-5	0.0
64-65	0.15988107570697774	1.586672710831913E-5	0.0	3.808014505996591E-5	0.0
66-67	0.18882357262526267	1.586672710831913E-5	0.0	3.808014505996591E-5	0.0
68-69	0.22311632992447278	1.9040072529982956E-5	0.0	3.808014505996591E-5	0.0
70-71	0.26378751152122726	1.9040072529982956E-5	0.0	3.9666817770797825E-5	0.0
72-73	0.3091488976512008	1.9040072529982956E-5	0.0	4.1253490481629744E-5	0.0
74-75	0.3637240922129753	1.9040072529982956E-5	0.0	4.601350861412548E-5	0.0
76-77	0.42841115196088153	1.9040072529982956E-5	0.0	4.7600181324957394E-5	0.0
78-79	0.5024310205939011	1.9040072529982956E-5	0.0	4.9186854035789306E-5	0.0
80-81	0.5868721555916647	1.9040072529982956E-5	0.0	5.077352674662122E-5	0.0
82-83	0.6831244822488611	2.2213417951646784E-5	0.0	5.077352674662122E-5	0.0
84-85	0.8069357272204969	2.2213417951646784E-5	0.0	5.7120217589948875E-5	0.0
86-87	0.9527810961274554	2.2213417951646784E-5	0.0	6.346690843327652E-5	0.0
88-89	1.1135078683893065	2.3800090662478697E-5	0.0	6.505358114410844E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	85135	0.0	89.83419	1
GCGTGTT	92955	0.0	81.89019	2
GTAAACG	27400	0.0	53.130302	1
TAAACGC	29365	0.0	49.473743	2
CGTGTTT	159370	0.0	47.769745	3
TCGAACG	4940	0.0	43.935394	2
CGAACGT	9150	0.0	41.44112	1
CGCGTTT	1320	0.0	40.05793	1
GTCGAAC	5850	0.0	37.62059	1
CGCAGGA	13075	0.0	36.72471	1
AAACGCT	42025	0.0	34.98801	3
GTGTTTT	221370	0.0	34.123104	4
AGTAAAC	17685	0.0	33.158318	1
ACGCTTC	45285	0.0	32.312935	5
AACGCTT	47015	0.0	31.224546	4
GTTTTTT	532635	0.0	30.5127	1
CGCTTCG	48495	0.0	30.044937	6
GAACGTT	12795	0.0	29.991375	2
CACGCGT	695	0.0	29.472918	1
TCGCAGG	8575	0.0	29.187828	2
>>END_MODULE