FastQCFastQC Report
Fri 27 May 2016
SRR2032144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31512485
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT4414921.4010066169012059No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2308580.7325921773544676No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1054260.33455311442433056No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT795400.2524078948391407No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC791720.25124010372396843No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC770410.24447770463040283No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT729620.23153362865543609No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC704220.22347333128440997No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT688280.21841501868227783No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT644750.2046014460617752No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC618550.19628728105701596No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC592010.1878652223079202No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC570220.1809505026341147No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC543830.17257604406634386No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA515570.1636081699047219No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA490930.15578904678574224No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT469000.14882990027603346No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA462490.1467640524065303No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG452810.14369225403835972No Hit
CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT413680.13127495340338918No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC411600.1306148975556831No Hit
GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA407820.12941537298629416No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG403620.12808256790919537No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG396250.12574381233342913No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA390910.12404924587826062No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC390200.1238239383533225No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT389590.123630364282601No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG362460.11502107815362704No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG354310.11243480163497102No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCC353750.11225709429135786No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC349190.11081004877907916No Hit
CCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGC347380.110235673257758No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA346200.10986121849800166No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT335210.10637371187959312No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT334250.1060690707191134No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCG334040.10600243046525845No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT327780.1040159162312969No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA325080.10315911296744766No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG320850.10181678785408388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA723000.063.122491
CCAAACG836950.054.93388
CCGAAAC835300.054.714852
CGAAACC914750.049.868723
CAAACGA964500.047.7185339
CGCTCGT23000.046.101891
AAACCAA1043650.044.2603455
GAAACCA1065950.043.0634424
ACCAAAC1122850.041.436347
ACTCGTC677650.040.8045442-43
GATATAT217900.040.4846085
TCGTCTA691750.039.9614844-45
CTAACGA677750.039.3525194-95
CCAGATA210650.038.5462072
TCCGCTC25150.038.5158232
TAGGTAG710950.037.74092576-77
AACCAAA1241700.037.67446
CAACTCG772650.036.0379240-41
CAGATAT231500.035.8741573
GTCCGTA15450.035.3924831
Produced by FastQC (version 0.11.5)