##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2032144_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31512485 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39459626874872 34.0 31.0 34.0 31.0 34.0 2 32.53070511576602 34.0 31.0 34.0 31.0 34.0 3 32.650488909395754 34.0 31.0 34.0 31.0 34.0 4 36.12664323362629 37.0 35.0 37.0 35.0 37.0 5 36.133198635398 37.0 35.0 37.0 35.0 37.0 6 36.038024611515084 37.0 35.0 37.0 35.0 37.0 7 35.92546960355554 37.0 35.0 37.0 35.0 37.0 8 35.86762402266911 37.0 35.0 37.0 35.0 37.0 9 37.645215666108214 39.0 38.0 39.0 35.0 39.0 10-11 37.77911592817894 39.0 38.0 39.0 35.0 39.0 12-13 37.694681504806745 39.0 38.0 39.0 35.0 39.0 14-15 39.11836613329606 41.0 39.0 41.0 36.0 41.0 16-17 39.09060481901062 41.0 39.0 41.0 36.0 41.0 18-19 39.0961688676726 41.0 39.0 41.0 36.0 41.0 20-21 39.070109482003716 40.5 39.0 41.0 36.0 41.0 22-23 39.0225077457395 40.0 39.0 41.0 35.5 41.0 24-25 39.00369137819502 40.0 39.0 41.0 35.0 41.0 26-27 38.94917708013189 40.0 39.0 41.0 35.0 41.0 28-29 38.91287005769301 40.0 38.0 41.0 35.0 41.0 30-31 38.83429440743883 40.0 38.0 41.0 35.0 41.0 32-33 38.76986707014696 40.0 38.0 41.0 35.0 41.0 34-35 38.675115735874215 40.0 38.0 41.0 35.0 41.0 36-37 38.55869540279035 40.0 38.0 41.0 34.0 41.0 38-39 38.2400425894689 40.0 38.0 41.0 33.5 41.0 40-41 37.945969256312225 40.0 38.0 41.0 33.0 41.0 42-43 37.86174581281038 40.0 37.5 41.0 33.0 41.0 44-45 37.802354558835965 40.0 37.0 41.0 33.0 41.0 46-47 37.678285590615914 40.0 37.0 41.0 32.5 41.0 48-49 37.74093117378715 40.0 37.0 41.0 32.5 41.0 50-51 38.08935667878937 40.0 37.0 41.0 33.0 41.0 52-53 38.07379100696121 40.0 37.0 41.0 33.0 41.0 54-55 37.968415502617454 40.0 37.0 41.0 33.0 41.0 56-57 37.86180258395998 40.0 37.0 41.0 33.0 41.0 58-59 37.68358837774933 40.0 36.5 41.0 33.0 41.0 60-61 37.48401888965596 39.0 36.0 41.0 33.0 41.0 62-63 37.195465908194805 39.0 36.0 41.0 32.0 41.0 64-65 36.82943466692646 38.5 35.0 40.5 31.5 41.0 66-67 36.50010890921487 37.5 35.0 40.0 31.0 41.0 68-69 36.083565577262476 37.0 35.0 39.5 31.0 41.0 70-71 35.74162643790231 37.0 35.0 39.0 31.0 41.0 72-73 35.42841538837702 36.0 34.5 39.0 31.0 41.0 74-75 35.11150369448808 36.0 34.0 38.5 31.0 40.0 76-77 34.996382322752396 35.5 34.0 37.5 31.0 40.0 78-79 34.644777887240565 35.0 34.0 37.0 31.0 39.0 80-81 34.28078851921707 35.0 34.0 36.5 30.5 39.0 82-83 33.99367372963446 35.0 34.0 36.0 30.0 38.0 84-85 33.777530334405554 35.0 34.0 36.0 30.0 37.0 86-87 33.52943525399536 35.0 34.0 35.5 30.0 37.0 88-89 33.41116668520429 35.0 34.0 35.0 30.0 36.5 90-91 33.32094696752731 35.0 34.0 35.0 30.0 36.0 92-93 33.199416326576596 35.0 34.0 35.0 30.0 36.0 94-95 33.07445184027854 35.0 34.0 35.0 30.0 36.0 96-97 32.956889800978885 35.0 34.0 35.0 30.0 36.0 98-99 32.8491483454891 35.0 34.0 35.0 30.0 36.0 100-101 32.05827209437783 34.5 33.0 35.0 27.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24606837218009758 1101 2 0.307333020143318 1101 3 0.24264605665410954 1101 4 0.16996295076190648 1101 5 0.14054680326211155 1101 6 0.15251448937060275 1101 7 0.16958694024645382 1101 8 0.12027148229594076 1101 9 0.15654389145326775 1101 10-11 0.1732072302934924 1101 12-13 0.17907082772173055 1101 14-15 0.2554009020740011 1101 16-17 0.22313704885491603 1101 18-19 0.26272360358600366 1101 20-21 0.2855871441637774 1101 22-23 0.2512413718423403 1101 24-25 0.3045412420588818 1101 26-27 0.2784330150929293 1101 28-29 0.3011838190459457 1101 30-31 0.2873363702302001 1101 32-33 0.28583486378094136 1101 34-35 0.32946901030911846 1101 36-37 0.3794160824947568 1101 38-39 0.3435835660908708 1101 40-41 0.29262810257597494 1101 42-43 0.22921678628893716 1101 44-45 0.20092211558177553 1101 46-47 0.23869812880940344 1101 48-49 0.22896952636333623 1101 50-51 0.23973560889762524 1101 52-53 0.23063793687980905 1101 54-55 0.2533682419898682 1101 56-57 0.30117733753328935 1101 58-59 0.2741626450598176 1101 60-61 0.27154818051647567 1101 62-63 0.29879662656644257 1101 64-65 0.31030234144974855 1101 66-67 0.2677834286232539 1101 68-69 0.20208348617106964 1101 70-71 0.24403778488134265 1101 72-73 0.22248412374698745 1101 74-75 0.2751574745530547 1101 76-77 0.32418395513163745 1101 78-79 0.32755537217027353 1101 80-81 0.33528747366206346 1101 82-83 0.3262623190750986 1101 84-85 0.3200248713224383 1101 86-87 0.34436432264587324 1101 88-89 0.29938098721051887 1101 90-91 0.30689998047232336 1101 92-93 0.30742939964455474 1101 94-95 0.3169785386438875 1101 96-97 0.3469667830306449 1101 98-99 0.36110712710085835 1101 100-101 0.36135824280489004 1102 1 0.128779304908484 1102 2 0.18221304806409222 1102 3 0.13139921955438183 1102 4 0.10430550376774761 1102 5 0.11129542903647405 1102 6 0.11383985364945914 1102 7 0.10200993998469698 1102 8 0.08451980268682746 1102 9 0.10203294127440188 1102 10-11 0.13990952503217358 1102 12-13 0.1425993156372698 1102 14-15 0.2048926567201903 1102 16-17 0.2118923930657175 1102 18-19 0.21126648815104687 1102 20-21 0.2135602704180286 1102 22-23 0.227595965682319 1102 24-25 0.23301606973535627 1102 26-27 0.2358102073100099 1102 28-29 0.23547183928850757 1102 30-31 0.25835765982098025 1102 32-33 0.24257947594392704 1102 34-35 0.2654039200028606 1102 36-37 0.2922291005560069 1102 38-39 0.3144194442864858 1102 40-41 0.3013424381487866 1102 42-43 0.2153436513094391 1102 44-45 0.16579210947411127 1102 46-47 0.14146621187370556 1102 48-49 0.1480101859916374 1102 50-51 0.1678178006784421 1102 52-53 0.18994558891715485 1102 54-55 0.15961551535170315 1102 56-57 0.20072798756382326 1102 58-59 0.24519484120325785 1102 60-61 0.24205686213238664 1102 62-63 0.2316449818583024 1102 64-65 0.22000699200381035 1102 66-67 0.18695496688344093 1102 68-69 0.19431802067940396 1102 70-71 0.2085478625360011 1102 72-73 0.15368558958765988 1102 74-75 0.20157096377738526 1102 76-77 0.2717016237469423 1102 78-79 0.3151109955888245 1102 80-81 0.3049999769340346 1102 82-83 0.28319234263324944 1102 84-85 0.2508227947148072 1102 86-87 0.2705705873697184 1102 88-89 0.26317123238981566 1102 90-91 0.27248980684000657 1102 92-93 0.27042346647991167 1102 94-95 0.2695895673499322 1102 96-97 0.2901872698991568 1102 98-99 0.26911035544044637 1102 100-101 0.3304490725754192 1103 1 0.2253063583351249 1103 2 0.2720176683168063 1103 3 0.18426032532349979 1103 4 0.1287922921418172 1103 5 0.10183698026177979 1103 6 0.09131465234077751 1103 7 0.06819922222043573 1103 8 0.07087407625479614 1103 9 0.09281544559875243 1103 10-11 0.1056935486687749 1103 12-13 0.12541619398138693 1103 14-15 0.15875522055314661 1103 16-17 0.15251753206870688 1103 18-19 0.16306384909901794 1103 20-21 0.143949428190858 1103 22-23 0.15940626720718143 1103 24-25 0.20751027943859413 1103 26-27 0.2000169426913203 1103 28-29 0.18607786412130167 1103 30-31 0.1758755235044802 1103 32-33 0.20285390152292138 1103 34-35 0.25169151925448574 1103 36-37 0.24254139660604324 1103 38-39 0.22597289995619008 1103 40-41 0.1998680573606606 1103 42-43 0.15876555793860092 1103 44-45 0.13535007778862962 1103 46-47 0.15829241032371044 1103 48-49 0.15424850519605116 1103 50-51 0.15348646912894282 1103 52-53 0.13069321140479673 1103 54-55 0.1657438885861069 1103 56-57 0.17868566760384397 1103 58-59 0.1787347191443729 1103 60-61 0.1793266254578043 1103 62-63 0.17787143637819014 1103 64-65 0.17703826322665606 1103 66-67 0.201866043313899 1103 68-69 0.20281312012357944 1103 70-71 0.21788802016457964 1103 72-73 0.15236365325110057 1103 74-75 0.17472887068663567 1103 76-77 0.1717491670266611 1103 78-79 0.22752756281677478 1103 80-81 0.23596726195145834 1103 82-83 0.24426594720263495 1103 84-85 0.2546485665771172 1103 86-87 0.23172559916771718 1103 88-89 0.22464756749587877 1103 90-91 0.19879968928711378 1103 92-93 0.20004418844376204 1103 94-95 0.20521586591121377 1103 96-97 0.23818470166560957 1103 98-99 0.2478209721534057 1103 100-101 0.29649746482343886 1104 1 0.2118033664727932 1104 2 0.21672299357615543 1104 3 0.16240852838395625 1104 4 0.09529850377731464 1104 5 0.06354511776698502 1104 6 0.09353798373064137 1104 7 0.1013084952047194 1104 8 0.09415336823302312 1104 9 0.11126662375313856 1104 10-11 0.11334860218158838 1104 12-13 0.1007363055001207 1104 14-15 0.17686430603059478 1104 16-17 0.13932271653427364 1104 18-19 0.19610697614530892 1104 20-21 0.1974594021871212 1104 22-23 0.17214397929927827 1104 24-25 0.2161885897990956 1104 26-27 0.20935451589738818 1104 28-29 0.19408835027378757 1104 30-31 0.2085907205885249 1104 32-33 0.20156364506745916 1104 34-35 0.219514070242262 1104 36-37 0.20723209294671818 1104 38-39 0.1686962610348317 1104 40-41 0.29568847238571294 1104 42-43 0.21657831128307237 1104 44-45 0.14503793799661224 1104 46-47 0.16338588702426904 1104 48-49 0.13800567557302656 1104 50-51 0.17841305658401296 1104 52-53 0.1759377267569633 1104 54-55 0.17723381458398535 1104 56-57 0.18372581266888233 1104 58-59 0.15579816595233353 1104 60-61 0.16227207041241343 1104 62-63 0.17755415785289586 1104 64-65 0.17767992107715003 1104 66-67 0.17766514158022062 1104 68-69 0.19167152975914092 1104 70-71 0.19191314001765392 1104 72-73 0.21607509807122938 1104 74-75 0.20633912137481758 1104 76-77 0.24046088545093625 1104 78-79 0.24940503483372822 1104 80-81 0.18786528731763497 1104 82-83 0.19231913814613932 1104 84-85 0.1382517313498539 1104 86-87 0.20679392677359942 1104 88-89 0.2094142573709732 1104 90-91 0.18210992444012675 1104 92-93 0.1923703287654348 1104 94-95 0.17036593335984662 1104 96-97 0.15120390262023164 1104 98-99 0.20493443972918612 1104 100-101 0.2835186204925577 1105 1 0.18395525883705943 1105 2 0.22398867426078084 1105 3 0.1480347837563869 1105 4 0.08211764871427363 1105 5 0.08279589152605382 1105 6 0.05706576016753928 1105 7 0.021131238927615925 1105 8 0.04594540798674984 1105 9 0.05775090758874768 1105 10-11 0.060462105200329574 1105 12-13 0.042161585763913934 1105 14-15 0.09490320878352065 1105 16-17 0.11658112794990672 1105 18-19 0.13499725552582476 1105 20-21 0.1265617883810748 1105 22-23 0.13472967278450199 1105 24-25 0.14866427103417834 1105 26-27 0.12408748828168825 1105 28-29 0.06598984846626621 1105 30-31 0.09364197521846762 1105 32-33 0.07956377568967099 1105 34-35 0.11562936519272426 1105 36-37 0.0829687816402469 1105 38-39 0.16575451093785887 1105 40-41 0.2384785521958932 1105 42-43 0.16544577571028185 1105 44-45 0.13770147524401466 1105 46-47 0.10381248553365197 1105 48-49 0.07865484025073499 1105 50-51 0.10199955285970219 1105 52-53 0.08349382637503311 1105 54-55 0.07750840296298378 1105 56-57 0.03007310377709871 1105 58-59 0.10291271688517156 1105 60-61 0.06739405516292152 1105 62-63 0.06803073651806102 1105 64-65 0.09121355717045532 1105 66-67 0.051076188758983676 1105 68-69 0.08189035774607589 1105 70-71 0.08805036014241807 1105 72-73 0.1157715131524597 1105 74-75 0.10558352379744917 1105 76-77 0.07007605174028697 1105 78-79 0.13460918288664203 1105 80-81 0.17048517449224931 1105 82-83 0.1312243416634189 1105 84-85 0.17027373503179177 1105 86-87 0.20364948491843649 1105 88-89 0.18256268989693325 1105 90-91 0.15559785895396772 1105 92-93 0.13979032471129216 1105 94-95 0.162734525749606 1105 96-97 0.1564561244611653 1105 98-99 0.15712904334449007 1105 100-101 0.20140808854612402 1106 1 0.1742283027638578 1106 2 0.21747965497140598 1106 3 0.12153915700721285 1106 4 0.06553097621680593 1106 5 0.05132238828435476 1106 6 0.056446564031197966 1106 7 0.056495574889765976 1106 8 0.033413852718432224 1106 9 0.030672545805934703 1106 10-11 0.036121636955392944 1106 12-13 0.04640951377444935 1106 14-15 0.07610364071766895 1106 16-17 0.08960255224240399 1106 18-19 0.09797915011569103 1106 20-21 0.11071467556815406 1106 22-23 0.09629508845910095 1106 24-25 0.15840778128618638 1106 26-27 0.15318564148631708 1106 28-29 0.15675769609920565 1106 30-31 0.09447725577491894 1106 32-33 0.11685265717395765 1106 34-35 0.13698783211874144 1106 36-37 0.17355534355299795 1106 38-39 0.16591327738694872 1106 40-41 0.2306689192731426 1106 42-43 0.17507068260524505 1106 44-45 0.15940375220775138 1106 46-47 0.13865063609576822 1106 48-49 0.10998467836595438 1106 50-51 0.062253402600340735 1106 52-53 0.13052468143650486 1106 54-55 0.1483105000044489 1106 56-57 0.15474831009035483 1106 58-59 0.10823689003467507 1106 60-61 0.1034197480307455 1106 62-63 0.08986913493185256 1106 64-65 0.0903230960179684 1106 66-67 0.10442281926554386 1106 68-69 0.12546007627781108 1106 70-71 0.13368893193224807 1106 72-73 0.07315830485118369 1106 74-75 0.10317028774103676 1106 76-77 0.14427460527106462 1106 78-79 0.15104545682793002 1106 80-81 0.18798280179646554 1106 82-83 0.16566119657955625 1106 84-85 0.1436510252744938 1106 86-87 0.1654331223881229 1106 88-89 0.16483454739900338 1106 90-91 0.16901005462564456 1106 92-93 0.1933827673109647 1106 94-95 0.19358497719812817 1106 96-97 0.21989645944287162 1106 98-99 0.23017995223595733 1106 100-101 0.3416984256526234 1107 1 0.16000621535079063 1107 2 0.18438583358297223 1107 3 0.08653947650424954 1107 4 0.05910867098202033 1107 5 0.03226360651451188 1107 6 -1.4566329994636362E-5 1107 7 0.03248460968497113 1107 8 0.006947209104048113 1107 9 -0.009003937117988414 1107 10-11 0.036066956863052724 1107 12-13 0.011879815010644279 1107 14-15 0.029694477496462923 1107 16-17 0.05262316709231385 1107 18-19 0.063150992126765 1107 20-21 0.08764428546624714 1107 22-23 0.07563816947862279 1107 24-25 0.13381957328548566 1107 26-27 0.1358110839848763 1107 28-29 0.10711221940917426 1107 30-31 0.1301552405433526 1107 32-33 0.11960761219022231 1107 34-35 0.11631749466496899 1107 36-37 0.11584704095983511 1107 38-39 0.019397244837804806 1107 40-41 0.23063526004778367 1107 42-43 0.21030317342789573 1107 44-45 0.23987688536722374 1107 46-47 0.17457925683209652 1107 48-49 0.13542847230945654 1107 50-51 0.1547367835226936 1107 52-53 0.15027214634277897 1107 54-55 0.10726194266992906 1107 56-57 0.1599785075965201 1107 58-59 0.17943134506898417 1107 60-61 0.17867283014673063 1107 62-63 0.1431697124101774 1107 64-65 0.1655483566706124 1107 66-67 0.16508871590052365 1107 68-69 0.17447596929502396 1107 70-71 0.17438800275716915 1107 72-73 0.1585738377115149 1107 74-75 0.16099691122497006 1107 76-77 0.1403177233323163 1107 78-79 0.19405157745906365 1107 80-81 0.16301350337222686 1107 82-83 0.1840009341977833 1107 84-85 0.12981390198235232 1107 86-87 0.15328981331197156 1107 88-89 0.19530786908247677 1107 90-91 0.20892811727327398 1107 92-93 0.20930403352868865 1107 94-95 0.20251226482739781 1107 96-97 0.19598521885177433 1107 98-99 0.23449923458993993 1107 100-101 0.33399864160256953 1108 1 0.15173466884298392 1108 2 0.17601404695418665 1108 3 0.08068432459644015 1108 4 0.04580845301530445 1108 5 0.045749845153657986 1108 6 0.038670261424393004 1108 7 0.02670138213041895 1108 8 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64-65 -0.08123299927397198 2214 66-67 -0.053274254031755675 2214 68-69 -0.009421063617473635 2214 70-71 0.026555245937402105 2214 72-73 0.018589179417247692 2214 74-75 -0.00565777049116889 2214 76-77 -0.05279596363707384 2214 78-79 -0.04322662346623929 2214 80-81 -0.061698432110823376 2214 82-83 -0.06152110812659117 2214 84-85 -0.04615039343089933 2214 86-87 9.353911580234353E-5 2214 88-89 0.013594147346047691 2214 90-91 0.004917354966138987 2214 92-93 -0.004182595675125356 2214 94-95 0.03177865038816918 2214 96-97 0.03131302805591929 2214 98-99 0.03554068052480375 2214 100-101 0.25482044358430755 2215 1 -0.04790647714477103 2215 2 -0.08091885549750089 2215 3 -0.13513043055624507 2215 4 -0.116674330368987 2215 5 -0.14912961543132752 2215 6 -0.26330795527184137 2215 7 -0.21806663170654872 2215 8 -0.16544111569936604 2215 9 -0.2011534891978286 2215 10-11 -0.2194040722396977 2215 12-13 -0.20087651760719893 2215 14-15 -0.2876050822229601 2215 16-17 -0.29506650963028136 2215 18-19 -0.3084657666870996 2215 20-21 -0.32605752185700254 2215 22-23 -0.3284466631412215 2215 24-25 -0.3114318038382038 2215 26-27 -0.3003256378466119 2215 28-29 -0.29707241675335183 2215 30-31 -0.3095259680025464 2215 32-33 -0.30181087617827984 2215 34-35 -0.25552707139130604 2215 36-37 -0.2505960897009345 2215 38-39 -0.3222554330690457 2215 40-41 -0.38088865551448947 2215 42-43 -0.20909940419193163 2215 44-45 -0.13672915001231445 2215 46-47 -0.006962172197958694 2215 48-49 -0.03867793705667566 2215 50-51 -0.243994342690236 2215 52-53 -0.23988971227137768 2215 54-55 -0.2588694361897481 2215 56-57 -0.25601330090299257 2215 58-59 -0.25846727231467526 2215 60-61 -0.3674855784565736 2215 62-63 -0.3055108710313661 2215 64-65 -0.2783923320736008 2215 66-67 -0.30209806520983307 2215 68-69 -0.2967337033503412 2215 70-71 -0.21627246406207234 2215 72-73 -0.24214944077360911 2215 74-75 -0.27442802095777097 2215 76-77 -0.32923258289351764 2215 78-79 -0.32914568845930603 2215 80-81 -0.2735749108673531 2215 82-83 -0.229079486384542 2215 84-85 -0.26751480539049055 2215 86-87 -0.23888112023168162 2215 88-89 -0.2174869179454575 2215 90-91 -0.23840192790462567 2215 92-93 -0.270921388522531 2215 94-95 -0.2810447321992271 2215 96-97 -0.2450641554587918 2215 98-99 -0.25572173897695194 2215 100-101 -0.025851289896635876 2216 1 -0.24994774285387678 2216 2 -0.2262030893983038 2216 3 -0.3129407272666924 2216 4 -0.2495825277947219 2216 5 -0.22092891100666634 2216 6 -0.3716329988556666 2216 7 -0.37575675829031496 2216 8 -0.30335293842360755 2216 9 -0.3836961098332452 2216 10-11 -0.4235734504399744 2216 12-13 -0.4797371890669808 2216 14-15 -0.6596268052255638 2216 16-17 -0.6188480085639512 2216 18-19 -0.6083530802504598 2216 20-21 -0.5873964231563775 2216 22-23 -0.6268691175194192 2216 24-25 -0.6250350648581886 2216 26-27 -0.6319954120063898 2216 28-29 -0.6524336773181858 2216 30-31 -0.65376110362849 2216 32-33 -0.718110810500967 2216 34-35 -0.7892399984178056 2216 36-37 -0.7059383469321716 2216 38-39 -0.6556598519823424 2216 40-41 -0.7216126881411 2216 42-43 -0.6446473491016036 2216 44-45 -0.42581877013901703 2216 46-47 -0.3469422484389142 2216 48-49 -0.40069066238778817 2216 50-51 -0.6986215825956705 2216 52-53 -0.7494220599185013 2216 54-55 -0.8609846158364718 2216 56-57 -0.765747155487233 2216 58-59 -0.7970304593840964 2216 60-61 -0.8071290604456962 2216 62-63 -0.8003215073984578 2216 64-65 -0.8103873225634928 2216 66-67 -0.8477890842605689 2216 68-69 -0.8020555135988303 2216 70-71 -0.7407469953317687 2216 72-73 -0.7710242493962696 2216 74-75 -0.8081679979042065 2216 76-77 -0.7837086050441542 2216 78-79 -0.8421767761089072 2216 80-81 -0.6488151468248589 2216 82-83 -0.7035245851317384 2216 84-85 -0.7802900483860853 2216 86-87 -0.6303484166253952 2216 88-89 -0.6560158760989125 2216 90-91 -0.7805537110349263 2216 92-93 -0.7341190946132059 2216 94-95 -0.7662704282944972 2216 96-97 -0.7634119528773127 2216 98-99 -0.809279334905824 2216 100-101 -0.5032299639255626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2537.0 3 1231.0 4 1359.0 5 2011.0 6 3577.0 7 6517.0 8 9970.0 9 12090.0 10 15090.0 11 18693.0 12 22423.0 13 23288.0 14 24201.0 15 26860.0 16 29181.0 17 33463.0 18 33535.0 19 35778.0 20 39865.0 21 46006.0 22 55086.0 23 69466.0 24 88230.0 25 115213.0 26 149876.0 27 192269.0 28 245577.0 29 314133.0 30 405764.0 31 531066.0 32 717459.0 33 1009362.0 34 1475442.0 35 2201518.0 36 3665719.0 37 6929255.0 38 9917750.0 39 3023273.0 40 18352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.661542326096626 10.0020861525389 6.050442945952443 53.285928575412036 2 21.39771136984606 12.880532363553279 38.09858055425976 27.623175712340895 3 22.685034201523617 15.876875466977614 21.821287657891787 39.61680267360698 4 24.287820695306493 25.80370792468375 20.386524346644656 29.5219470333651 5 22.965206408179757 33.428483569961635 23.468748049132397 20.13756197272621 6 18.969554470238247 37.39769159244143 24.481775937767758 19.15097799955256 7 15.365685300323998 28.222416943223738 39.341391651462246 17.070506104990024 8 17.26627398632637 24.614675738838116 32.68480413398055 25.434246140854967 9 16.369943531904894 24.08016060935848 35.92317775002511 23.626718108711515 10-11 19.95059416758703 35.4612624769259 25.213755402731568 19.374387952755498 12-13 21.07511337966075 28.710571703036948 27.817451268923826 22.39686364837848 14-15 21.08191022154473 31.325489217188395 26.139482694398332 21.453117866868542 16-17 21.396734607899305 28.79854675984076 27.053509500157062 22.751209132102872 18-19 20.705282366655627 29.400953304698124 27.046922830744702 22.846841497901547 20-21 21.507940424247725 28.66782165862197 25.87153313996024 23.952704777170062 22-23 21.29384591535704 30.205973917956648 26.526138766904612 21.974041399781704 24-25 20.861469922837866 30.799301277073994 26.083795786972143 22.255433013116 26-27 20.548496326276176 31.86390462552144 26.479168665550922 21.10843038265146 28-29 20.606181272806978 30.2734090547509 26.14539066930492 22.975019003137202 30-31 19.48517316838474 29.575765232204258 28.104542652037868 22.83451894737313 32-33 20.48819008181902 28.324561750912064 29.224965114863586 21.962283052405326 34-35 20.235036556298553 30.451343698006905 26.781580860460767 22.532038885233774 36-37 21.115929274519672 29.887171198956846 26.928430479413123 22.068469047110362 38-39 20.35874744050138 30.10556306772419 26.355802255718324 23.179887236056107 40-41 20.58690556026452 30.098408521586595 25.881306599992023 23.433379318156863 42-43 19.91270759159203 30.649027431697757 26.858824660956227 22.57944031575398 44-45 19.797640836602394 28.60766183002803 27.19416014310138 24.400537190268192 46-47 20.32155950622324 29.781976403879334 26.806034675359093 23.09042941453833 48-49 19.382973848792528 29.055293433890643 27.71792442803209 23.843808289284738 50-51 19.065351212614953 29.637325949745936 27.92696668800164 23.37035614963747 52-53 21.315575550122126 28.79510489243082 27.32112741459305 22.568192142854006 54-55 20.51565265909285 29.03050626505414 26.785273273076964 23.668567802776046 56-57 21.795352525770127 28.331523017021272 26.29579036116576 23.57733409604284 58-59 19.996366913774196 31.25347744205017 26.618424445257972 22.131731198917663 60-61 19.39095357954169 31.972763329481097 25.98345440826198 22.652828682715224 62-63 19.93414889237125 30.797820442673235 27.275167145027808 21.99286351992771 64-65 21.226524927963712 29.956525706554256 26.26041186344769 22.55653750203434 66-67 21.411061550753153 29.95406540856577 26.40079305881234 22.234079981868735 68-69 20.414746127729078 29.933639927765515 25.774857601112007 23.876756343393403 70-71 20.332340399213273 31.121442975541413 25.911651912494314 22.634564712750997 72-73 19.258750721580466 30.802192789036443 26.807737865456577 23.131318623926518 74-75 19.394847282064042 29.20628967818167 28.254129939079935 23.144733100674355 76-77 18.616097764534334 29.074973542203157 28.165909029605757 24.143019663656744 78-79 20.715329589225853 31.216285697866304 26.106272379621387 21.962112333286456 80-81 20.08396076616024 30.278910601284338 26.539714802178672 23.09741383037675 82-83 20.615849802461852 30.709235828713275 25.392193257351003 23.282721111473865 84-85 20.06667063336229 30.808877464805494 25.519165089905155 23.60528681192706 86-87 20.23844770656316 30.55724430640797 26.704125202094765 22.500182784934104 88-89 19.069789092115013 30.96838252851977 26.83015003199008 23.131678347375143 90-91 19.202048097246973 32.73617161851735 26.319892951741018 21.741887332494656 92-93 20.753458867229334 30.72934716024356 27.005608524406128 21.511585448120982 94-95 19.199338870456728 29.87098143491019 27.269317777598012 23.660361917035072 96-97 19.004500594994884 31.217712394955488 28.130448446567293 21.64733856348234 98-99 19.605521202170326 30.369096677682457 26.856231558811565 23.169150561335655 100-101 20.67748060164407 31.27799403983066 25.873644168761555 22.170881189763712 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 112.0 1 120.0 2 123.5 3 111.0 4 116.5 5 138.0 6 136.5 7 138.0 8 154.0 9 168.0 10 168.0 11 221.0 12 319.0 13 423.5 14 609.5 15 884.5 16 1378.5 17 2139.5 18 3339.0 19 5322.5 20 11194.5 21 20084.5 22 30596.0 23 57405.0 24 111575.0 25 152951.0 26 154818.5 27 202212.5 28 302401.0 29 386850.5 30 461076.5 31 543875.5 32 644681.5 33 737894.5 34 853962.5 35 1016137.5 36 1191103.0 37 1369022.5 38 1633916.0 39 1575615.5 40 1375895.5 41 1505893.5 42 1338070.0 43 1035995.0 44 1002919.0 45 1016866.5 46 992999.0 47 985832.5 48 977741.0 49 974842.5 50 943789.0 51 839673.0 52 778840.0 53 736173.0 54 710829.5 55 684836.5 56 633279.0 57 545452.5 58 455177.0 59 436031.5 60 405657.5 61 316008.0 62 241981.0 63 183928.5 64 137031.0 65 109643.5 66 81912.0 67 56397.5 68 33296.5 69 24119.5 70 36403.0 71 72449.0 72 71005.5 73 139261.5 74 129196.0 75 15579.0 76 5930.0 77 4064.0 78 1748.5 79 974.5 80 458.0 81 278.5 82 227.0 83 148.0 84 82.0 85 46.0 86 32.0 87 20.5 88 9.5 89 7.5 90 4.0 91 1.0 92 2.5 93 3.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.136675987311061 2 5.870689030078078E-4 3 0.0 4 0.010202305530649202 5 0.012909169175328444 6 0.014102347053874042 7 0.009567636446316436 8 0.0 9 0.0 10-11 0.0013201116954121518 12-13 0.0010456173164382309 14-15 6.441891205977567E-4 16-17 1.9040072529982956E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.347483227679442E-4 26-27 3.601747053588443E-4 28-29 0.010199132185227538 30-31 3.871481414429868E-4 32-33 0.0015771526745669217 34-35 0.0034557731641919067 36-37 0.001150337715353137 38-39 0.34704340200399936 40-41 0.49198912748391627 42-43 0.04655773735394083 44-45 1.586672710831913E-6 46-47 0.0413407574807255 48-49 0.010015078150771036 50-51 2.2372085222729975E-4 52-53 0.00116937778788312 54-55 5.950022665619675E-4 56-57 0.014722736083809322 58-59 0.032820325023558125 60-61 1.3645385313154452E-4 62-63 9.837370807157861E-5 64-65 0.001315351677279656 66-67 2.1578748867314017E-4 68-69 0.0012328446963163963 70-71 4.950418857795569E-4 72-73 0.002940104533171535 74-75 3.9825485041881015E-4 76-77 1.1106708975823391E-4 78-79 0.02525506953831156 80-81 4.680684496954144E-4 82-83 0.001894487216733304 84-85 3.490679963830209E-4 86-87 1.3010716228821687E-4 88-89 0.023495449501998972 90-91 0.03073067706339249 92-93 0.014451415050257066 94-95 0.026086486038787486 96-97 0.014759229556158457 98-99 4.6013508614125485E-4 100-101 0.025421670172948913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.1512485E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.213334219444526 #Duplication Level Percentage of deduplicated Percentage of total 1 70.54772851028353 10.027184437392952 2 10.689588322838208 3.0386938300154185 3 4.6000800407603695 1.961474251665694 4 2.7059642801496326 1.538430987985814 5 1.7956289251624606 1.2760937023718 6 1.3059691898338919 1.113730594524378 7 0.9536262474950229 0.948794600325704 8 0.7854983368301655 0.8931640312147962 9 0.6453887630778724 0.8255813571989729 >10 4.743776938569327 13.657017340778765 >50 0.6134803342307797 6.084031699443057 >100 0.48550682336309076 13.92297646069845 >500 0.06326659764863966 6.244116734691742 >1k 0.05301932154209299 15.158410575317985 >5k 0.006805562757891871 6.6550347080226935 >10k+ 0.004671805457562735 16.655264688351696 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 441492 1.4010066169012059 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 230858 0.7325921773544676 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 105426 0.33455311442433056 No Hit CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT 79540 0.2524078948391407 No Hit CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC 79172 0.25124010372396843 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 77041 0.24447770463040283 No Hit CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT 72962 0.23153362865543609 No Hit GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC 70422 0.22347333128440997 No Hit GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT 68828 0.21841501868227783 No Hit CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT 64475 0.2046014460617752 No Hit GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 61855 0.19628728105701596 No Hit CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC 59201 0.1878652223079202 No Hit CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC 57022 0.1809505026341147 No Hit GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC 54383 0.17257604406634386 No Hit CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA 51557 0.1636081699047219 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 49093 0.15578904678574224 No Hit GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT 46900 0.14882990027603346 No Hit CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA 46249 0.1467640524065303 No Hit CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG 45281 0.14369225403835972 No Hit CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT 41368 0.13127495340338918 No Hit CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC 41160 0.1306148975556831 No Hit GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA 40782 0.12941537298629416 No Hit GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG 40362 0.12808256790919537 No Hit CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG 39625 0.12574381233342913 No Hit CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA 39091 0.12404924587826062 No Hit CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC 39020 0.1238239383533225 No Hit CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT 38959 0.123630364282601 No Hit ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG 36246 0.11502107815362704 No Hit CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG 35431 0.11243480163497102 No Hit GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCC 35375 0.11225709429135786 No Hit CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC 34919 0.11081004877907916 No Hit CCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGC 34738 0.110235673257758 No Hit CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA 34620 0.10986121849800166 No Hit CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT 33521 0.10637371187959312 No Hit CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT 33425 0.1060690707191134 No Hit CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCG 33404 0.10600243046525845 No Hit CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT 32778 0.1040159162312969 No Hit CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA 32508 0.10315911296744766 No Hit ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG 32085 0.10181678785408388 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.173345421663826E-6 0.0 0.0 3.173345421663826E-5 0.0 2 3.173345421663826E-6 0.0 0.0 6.981359927660417E-5 0.0 3 3.173345421663826E-6 0.0 0.0 7.616029011993182E-5 3.173345421663826E-6 4 9.520036264991478E-6 0.0 0.0 8.250698096325949E-5 3.173345421663826E-6 5 9.520036264991478E-6 0.0 0.0 8.250698096325949E-5 3.173345421663826E-6 6 9.520036264991478E-6 0.0 0.0 8.250698096325949E-5 3.173345421663826E-6 7 9.520036264991478E-6 0.0 0.0 9.520036264991479E-5 3.173345421663826E-6 8 9.520036264991478E-6 0.0 0.0 9.520036264991479E-5 3.173345421663826E-6 9 9.520036264991478E-6 0.0 0.0 9.837370807157861E-5 3.173345421663826E-6 10-11 1.110670897582339E-5 0.0 0.0 1.5073390752903175E-4 3.173345421663826E-6 12-13 1.7453399819151044E-5 0.0 0.0 2.570409791547699E-4 3.173345421663826E-6 14-15 2.3800090662478697E-5 0.0 0.0 2.8084106981724865E-4 3.173345421663826E-6 16-17 3.6493472349134E-5 0.0 0.0 3.0940117861222305E-4 3.173345421663826E-6 18-19 5.394687216828505E-5 0.0 0.0 3.2050788758804645E-4 3.173345421663826E-6 20-21 8.56803263849233E-5 0.0 0.0 3.395479601180294E-4 3.173345421663826E-6 22-23 1.570805983723594E-4 0.0 0.0 3.554146872263485E-4 3.173345421663826E-6 24-25 3.268545784313741E-4 0.0 0.0 3.9349483228631445E-4 3.173345421663826E-6 26-27 5.474020852370099E-4 0.0 0.0 4.3633499547877615E-4 3.173345421663826E-6 28-29 8.23483136921763E-4 0.0 0.0 4.918685403578931E-4 3.173345421663826E-6 30-31 0.0012376047144488922 0.0 0.0 5.061485947553802E-4 3.173345421663826E-6 32-33 0.0019690608341424044 0.0 0.0 5.140819583095398E-4 3.173345421663826E-6 34-35 0.003195558839615473 0.0 0.0 5.251886672853633E-4 3.173345421663826E-6 36-37 0.004966285584903888 0.0 0.0 5.394687216828504E-4 3.173345421663826E-6 38-39 0.00787148331843712 0.0 0.0 5.505754306586738E-4 3.173345421663826E-6 40-41 0.011881005258709365 0.0 0.0 5.696155031886569E-4 3.173345421663826E-6 42-43 0.016525196283314374 0.0 0.0 5.823088848753122E-4 3.173345421663826E-6 44-45 0.022072204080382744 0.0 0.0 5.934155938511355E-4 3.173345421663826E-6 46-47 0.028668002539311007 0.0 0.0 6.140423390919503E-4 3.173345421663826E-6 48-49 0.036334805078050814 0.0 0.0 6.235623753569418E-4 3.173345421663826E-6 50-51 0.04484413082624236 0.0 0.0 6.251490480677738E-4 3.173345421663826E-6 52-53 0.054551394471112 0.0 0.0 6.251490480677738E-4 3.173345421663826E-6 54-55 0.06611506518765499 0.0 0.0 6.283223934894376E-4 3.173345421663826E-6 56-57 0.07952086292147381 0.0 0.0 6.457757933085887E-4 3.173345421663826E-6 58-59 0.09490682819841088 0.0 0.0 6.505358114410844E-4 3.173345421663826E-6 60-61 0.11210953372925049 0.0 0.0 6.632291931277397E-4 3.173345421663826E-6 62-63 0.13240942439163397 0.0 0.0 6.870292837902183E-4 3.173345421663826E-6 64-65 0.15633168885284673 0.0 0.0 6.965493200552098E-4 3.173345421663826E-6 66-67 0.18411432801951355 0.0 0.0 6.981359927660418E-4 3.173345421663826E-6 68-69 0.21684897271668674 0.0 0.0 6.981359927660418E-4 3.173345421663826E-6 70-71 0.25549555993441964 0.0 0.0 7.155893925851929E-4 3.173345421663826E-6 72-73 0.2984372701803745 0.0 0.0 7.695362647534778E-4 3.173345421663826E-6 74-75 0.35043888160517966 0.0 0.0 8.298298277650905E-4 3.173345421663826E-6 76-77 0.4119414892224463 0.0 0.0 9.028167724633585E-4 4.760018132495739E-6 78-79 0.482089876441036 0.0 0.0 9.361368993908287E-4 6.346690843327652E-6 80-81 0.5622771419010593 0.0 0.0 9.551769719208117E-4 6.346690843327652E-6 82-83 0.6532442617584744 0.0 0.0 9.662836808966352E-4 6.346690843327652E-6 84-85 0.7707405493409993 0.0 0.0 9.710436990291308E-4 6.346690843327652E-6 86-87 0.9096442251380683 0.0 0.0 9.773903898724584E-4 6.346690843327652E-6 88-89 1.0629850359309967 0.0 0.0 9.948437896916094E-4 6.346690843327652E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGAAA 72300 0.0 63.12249 1 CCAAACG 83695 0.0 54.9338 8 CCGAAAC 83530 0.0 54.71485 2 CGAAACC 91475 0.0 49.86872 3 CAAACGA 96450 0.0 47.718533 9 CGCTCGT 2300 0.0 46.10189 1 AAACCAA 104365 0.0 44.260345 5 GAAACCA 106595 0.0 43.063442 4 ACCAAAC 112285 0.0 41.43634 7 ACTCGTC 67765 0.0 40.80454 42-43 GATATAT 21790 0.0 40.484608 5 TCGTCTA 69175 0.0 39.96148 44-45 CTAACGA 67775 0.0 39.35251 94-95 CCAGATA 21065 0.0 38.546207 2 TCCGCTC 2515 0.0 38.515823 2 TAGGTAG 71095 0.0 37.740925 76-77 AACCAAA 124170 0.0 37.6744 6 CAACTCG 77265 0.0 36.03792 40-41 CAGATAT 23150 0.0 35.874157 3 GTCCGTA 1545 0.0 35.392483 1 >>END_MODULE