FastQCFastQC Report
Fri 27 May 2016
SRR2032143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25458372
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1448680.5690387429329731No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1107300.43494532957566967No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC990640.3891215039202035No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT921820.3620891390855629No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA921230.3618573882100552No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG915090.3594456079123991No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC807000.31698806192320544No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC803830.3157428919649693No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA799080.3138771010180855No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA765270.3005965974572137No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA664220.26090435005034884No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA660140.2593017338264992No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG658790.2587714563994901No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT624290.24521992215370253No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG586590.23041143400685635No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG585330.22991650840831457No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT570780.22420129613943893No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC550480.2162274948295987No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC541420.21266874409722664No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT511830.20104584849337578No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG504590.1982019902922308No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT500450.1965758061827363No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT489690.19234929869042688No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA449640.17661773502249084No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA445320.17492084725606177No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA436090.171295320847696No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA434270.17058042831646894No Hit
ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT431210.16937846614858168No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA418760.16448812987727573No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT397910.15629828961569106No Hit
AGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG389380.1529477218731818No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT386290.15173397576247216No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA355430.1396122265791387No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA344360.13526395167766422No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG331210.13009865674050172No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT329930.12959587518007828No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA328430.12900667803895707No Hit
CTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAATTAGCAAGAG325740.12795005116587974No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG322660.12674023303611087No Hit
ATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTA321920.12644956244649108No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG314050.1233582414460752No Hit
CAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACC309500.12157101011800754No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA306760.12049474334022617No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA305820.12012551313179021No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC303960.11939490867679992No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA299650.11770194889131166No Hit
CTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTGTGACTAGA298760.11735235858757977No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC297620.11690456876032765No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA288890.11347544139900226No Hit
TTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTG286590.11257200578261642No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC281100.11041554424611284No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT269470.10584730241195313No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC262070.10294059651575521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT494200.087.946751
GCGTGTT531700.081.160572
GTAAACG191500.053.5931661
TAAACGC207600.049.4386252
TCGAACG37950.046.5554052
GTCGAAC40550.043.3578761
CGTGTTT1025550.043.2353171
CGAACGT72850.042.839481
CGCAGGA86450.040.1235051
AAACGCT296400.034.96413
CGCGTTT10300.033.7689931
ACGCTTC312450.033.0621725
GAACGTT100150.031.7259142
GTTTTTT3887450.031.2639641
AACGCTT332000.031.2441644
TCGCAGG50850.031.102282
CGCTTCG340300.030.2539836
AGTAAAC134850.029.7504561
CCTTTAG121550.029.7359835
ACGTTTA113400.028.857624
Produced by FastQC (version 0.11.5)