##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032143_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25458372
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85341753196159	33.0	31.0	34.0	30.0	34.0
2	31.922653184579122	34.0	31.0	34.0	30.0	34.0
3	32.22774182104025	34.0	31.0	34.0	30.0	34.0
4	35.65893176515765	37.0	35.0	37.0	33.0	37.0
5	35.787147229995696	37.0	35.0	37.0	35.0	37.0
6	35.73640223341854	37.0	35.0	37.0	35.0	37.0
7	35.83032607112505	37.0	35.0	37.0	35.0	37.0
8	35.7909277938118	37.0	35.0	37.0	35.0	37.0
9	37.585928393221685	39.0	37.0	39.0	35.0	39.0
10-11	37.48303990137311	39.0	37.5	39.0	35.0	39.0
12-13	37.321198778932136	39.0	37.0	39.0	34.0	39.0
14-15	38.70469527666577	40.0	38.5	41.0	34.5	41.0
16-17	38.4928016999673	40.0	38.0	41.0	34.0	41.0
18-19	38.150065212339584	40.0	38.0	41.0	33.0	41.0
20-21	37.87244264872868	40.0	38.0	41.0	33.0	41.0
22-23	37.63700768061681	40.0	38.0	41.0	32.0	41.0
24-25	37.42950619544722	40.0	38.0	41.0	32.0	41.0
26-27	37.180892321001515	40.0	38.0	41.0	31.0	41.0
28-29	36.957335390495516	40.0	38.0	41.0	30.5	41.0
30-31	36.64916167852367	40.0	37.0	41.0	30.0	41.0
32-33	36.46697231857559	40.0	37.0	41.0	29.5	41.0
34-35	36.400011929278115	40.0	37.0	41.0	29.0	41.0
36-37	36.21495090102383	40.0	37.0	41.0	29.0	41.0
38-39	36.06473473637671	40.0	37.0	41.0	28.0	41.0
40-41	36.05117444273341	40.0	36.5	41.0	27.5	41.0
42-43	36.06083330858705	40.0	37.0	41.0	27.5	41.0
44-45	36.09169239101385	40.0	37.0	41.0	28.0	41.0
46-47	35.98067751543579	40.0	36.5	41.0	27.0	41.0
48-49	35.839596322184306	40.0	36.0	41.0	26.0	41.0
50-51	35.786775210920794	40.0	36.0	41.0	26.5	41.0
52-53	35.64560758244871	40.0	36.0	41.0	26.0	41.0
54-55	35.48313423183541	40.0	35.5	41.0	25.0	41.0
56-57	35.35632779268054	40.0	35.0	41.0	25.0	41.0
58-59	35.20686252050996	39.0	35.0	41.0	25.0	41.0
60-61	35.1265585442777	39.0	35.0	41.0	24.5	41.0
62-63	35.15265581396957	39.0	35.0	41.0	25.5	41.0
64-65	34.960799201928545	39.0	35.0	41.0	25.0	41.0
66-67	34.715635999819625	39.0	35.0	41.0	24.0	41.0
68-69	34.445491565603646	38.0	34.5	40.5	23.5	41.0
70-71	34.082936430499174	37.0	34.0	40.0	23.0	41.0
72-73	33.714417972209695	37.0	34.0	40.0	21.5	41.0
74-75	33.33320968049331	36.5	34.0	39.0	20.0	41.0
76-77	33.00943893820076	36.0	34.0	39.0	20.5	41.0
78-79	32.65782868990995	36.0	34.0	38.5	20.0	39.5
80-81	32.29359591414565	35.0	33.5	37.0	19.0	39.0
82-83	31.954492808888176	35.0	33.0	37.0	18.5	39.0
84-85	31.638951245586323	35.0	33.0	36.5	18.0	38.0
86-87	31.332884679350272	35.0	33.0	36.0	15.5	37.0
88-89	31.069995402691106	35.0	33.0	36.0	9.5	37.0
90-91	30.845248077921084	35.0	33.0	35.5	6.0	36.5
92-93	30.643881568703605	35.0	33.0	35.0	2.0	36.0
94-95	30.426655757877995	35.0	32.0	35.0	2.0	36.0
96-97	30.274545972539016	35.0	32.0	35.0	2.0	36.0
98-99	30.09375322585435	35.0	32.0	35.0	2.0	36.0
100-101	29.39967797233853	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3776721987394076
1101	2	0.4216528380630855
1101	3	0.22290132530476114
1101	4	0.3083074637375063
1101	5	0.20231696127846277
1101	6	0.18939619428196863
1101	7	0.12103144808169475
1101	8	0.11937919469551161
1101	9	0.192112216555131
1101	10-11	0.2295448009941481
1101	12-13	0.17182074294513683
1101	14-15	0.2222044667356471
1101	16-17	0.19315697175449031
1101	18-19	0.19689978962935584
1101	20-21	0.20832386972566042
1101	22-23	0.17635243283726965
1101	24-25	0.019691465122768648
1101	26-27	0.010477734513003156
1101	28-29	-0.005267251034190679
1101	30-31	0.01555230552209963
1101	32-33	-0.03894175199721417
1101	34-35	-0.0713023688434049
1101	36-37	-0.03548510136291583
1101	38-39	-0.06103256704418669
1101	40-41	-0.028071273234139937
1101	42-43	-0.058058354619277
1101	44-45	-0.11204651173333957
1101	46-47	-0.10793329861414946
1101	48-49	-0.03783431866221321
1101	50-51	-0.08340002134590208
1101	52-53	-0.13529843899680571
1101	54-55	-0.13096175806579424
1101	56-57	-0.09823968016599594
1101	58-59	-0.08505534179212049
1101	60-61	-0.06979545195193282
1101	62-63	-0.07614325883930206
1101	64-65	-0.053640515818024426
1101	66-67	-0.06878999550785636
1101	68-69	-0.05499886400546217
1101	70-71	-0.06796959083965248
1101	72-73	-0.09913213659088882
1101	74-75	-0.1021896465209906
1101	76-77	-0.15002193126105823
1101	78-79	-0.10846483771556592
1101	80-81	-0.09671647048037357
1101	82-83	-0.11495546665998191
1101	84-85	-0.11196617072252124
1101	86-87	-0.13063398840870732
1101	88-89	-0.09540331552325654
1101	90-91	-0.1171902403464955
1101	92-93	-0.09701898371287498
1101	94-95	-0.08540184780062887
1101	96-97	-0.07796931780637095
1101	98-99	-0.05241945408959836
1101	100-101	0.03609012000373113
1102	1	0.3280837244104511
1102	2	0.34814791711902515
1102	3	0.22244918679695758
1102	4	0.28996677174352214
1102	5	0.1818495247244627
1102	6	0.14492963191953123
1102	7	0.10648046497833974
1102	8	0.08746484449743264
1102	9	0.13847480030600678
1102	10-11	0.1752053951300212
1102	12-13	0.15497838609782377
1102	14-15	0.2029516456710425
1102	16-17	0.18708708967388077
1102	18-19	0.20408741881468018
1102	20-21	0.1682580305360375
1102	22-23	0.21634741248064415
1102	24-25	0.1397050631786385
1102	26-27	0.13141607465914262
1102	28-29	0.0957545659509833
1102	30-31	0.13240021320644502
1102	32-33	0.05195570378623415
1102	34-35	0.07571480955338927
1102	36-37	0.030417193099943063
1102	38-39	0.00390281735743514
1102	40-41	0.06119288619644436
1102	42-43	0.03690481948698476
1102	44-45	0.024171026542802565
1102	46-47	0.004415714427416617
1102	48-49	-0.024867964921405417
1102	50-51	0.027383545833991718
1102	52-53	0.052665510179764397
1102	54-55	-0.007897451783549059
1102	56-57	-0.027204322768426437
1102	58-59	0.023424395767349893
1102	60-61	0.04495213346878302
1102	62-63	0.07030587682769607
1102	64-65	0.08288545734818342
1102	66-67	0.08994125563165767
1102	68-69	0.05953505626441569
1102	70-71	0.002058691281007441
1102	72-73	-0.027140671176745457
1102	74-75	0.03154323662892722
1102	76-77	0.033075389251933984
1102	78-79	0.0303824415083227
1102	80-81	-0.027383110906434638
1102	82-83	-0.018613531341795664
1102	84-85	-0.031261235449335345
1102	86-87	-0.011208202133300205
1102	88-89	-0.00389144008086717
1102	90-91	-4.5755653800227947E-4
1102	92-93	0.013435928149803544
1102	94-95	-0.015722843579634826
1102	96-97	0.03010119701367131
1102	98-99	-0.008002427927142008
1102	100-101	0.08063148653459251
1103	1	0.30832414202542324
1103	2	0.33744795916310366
1103	3	0.18492975029412406
1103	4	0.24705890032592492
1103	5	0.10971731612069391
1103	6	0.11730751559590402
1103	7	0.09982925140729293
1103	8	0.0987790994895974
1103	9	0.10919440903489175
1103	10-11	0.20962379894368155
1103	12-13	0.12360498044188262
1103	14-15	0.18894019167516518
1103	16-17	0.20624701226427078
1103	18-19	0.22740809510604265
1103	20-21	0.1964267116653673
1103	22-23	0.18973462852883216
1103	24-25	0.13421939559859197
1103	26-27	0.11349136210345989
1103	28-29	0.07703561275963011
1103	30-31	0.10642797034402207
1103	32-33	0.08198138785616038
1103	34-35	0.027790064582347895
1103	36-37	0.02077237665417897
1103	38-39	0.009922281309030723
1103	40-41	0.0822188729632174
1103	42-43	0.07222034538988709
1103	44-45	-0.012682844376421087
1103	46-47	-0.0394003231847293
1103	48-49	-0.03052085691257389
1103	50-51	-0.052968608579242016
1103	52-53	-0.007093800759257363
1103	54-55	-0.007960504572338323
1103	56-57	-0.02050783126240674
1103	58-59	0.010812713682668118
1103	60-61	0.008306341821992191
1103	62-63	-0.00836797044152604
1103	64-65	0.012416996860793006
1103	66-67	0.045514871876164875
1103	68-69	0.04501733975678235
1103	70-71	0.06655085252556603
1103	72-73	-0.0014863439298622438
1103	74-75	0.025228533325751812
1103	76-77	-0.014717064718340112
1103	78-79	0.003358752022649014
1103	80-81	-0.01164094711629815
1103	82-83	-0.04768382860597953
1103	84-85	-0.05641157744276626
1103	86-87	-0.03736638500118872
1103	88-89	0.0014882790432437787
1103	90-91	-0.06147968415399774
1103	92-93	-0.035241468977311285
1103	94-95	-0.014154838079559084
1103	96-97	0.01105972806533373
1103	98-99	0.03210425427742081
1103	100-101	0.14987190044744025
1104	1	0.2676089854115524
1104	2	0.29135324509170246
1104	3	0.1558855858788064
1104	4	0.2342098091006406
1104	5	0.14272428682992455
1104	6	0.13656719457547695
1104	7	0.07920857610522347
1104	8	0.037371524456325744
1104	9	0.06066372099720496
1104	10-11	0.10177708666002871
1104	12-13	0.07075407548261836
1104	14-15	0.051240510302207554
1104	16-17	0.044604874615828294
1104	18-19	0.05654820319647058
1104	20-21	-0.002562664893531519
1104	22-23	0.05296981657340183
1104	24-25	0.0015911308430958115
1104	26-27	0.016207189937347266
1104	28-29	-0.039374924472660666
1104	30-31	-0.034989977335435185
1104	32-33	-0.10525301524186403
1104	34-35	-0.06844485121570187
1104	36-37	-0.09071183357510648
1104	38-39	-0.10209115769878707
1104	40-41	-0.10190908617609296
1104	42-43	-0.13680896123674557
1104	44-45	-0.17072494528889592
1104	46-47	-0.1363110939179677
1104	48-49	-0.10660312137203931
1104	50-51	-0.13121929248395503
1104	52-53	-0.16397081863514273
1104	54-55	-0.16824215278018784
1104	56-57	-0.14943793301599584
1104	58-59	-0.15334424389907753
1104	60-61	-0.11771609207571743
1104	62-63	-0.10845728989641401
1104	64-65	-0.12467073896641523
1104	66-67	-0.16257295924918935
1104	68-69	-0.1262902220803639
1104	70-71	-0.14863878165333233
1104	72-73	-0.15880299207339021
1104	74-75	-0.21385029225904617
1104	76-77	-0.15992843198431217
1104	78-79	-0.1813158451026311
1104	80-81	-0.17392302623228773
1104	82-83	-0.21281126378764625
1104	84-85	-0.19890767484233862
1104	86-87	-0.19185814636464116
1104	88-89	-0.13644871085021393
1104	90-91	-0.14501403487765074
1104	92-93	-0.18243521810945396
1104	94-95	-0.13422603555640578
1104	96-97	-0.14496119338060964
1104	98-99	-0.17892589728918296
1104	100-101	-0.0547392031699907
1105	1	0.18144861650356248
1105	2	0.2330172381629687
1105	3	0.06706120264982474
1105	4	0.1867980024608613
1105	5	0.08034509535073653
1105	6	0.11389676688666839
1105	7	0.053444873430578355
1105	8	0.08912774510309873
1105	9	0.096745409709726
1105	10-11	0.12043399990076864
1105	12-13	0.04313009343300678
1105	14-15	0.09331416869750342
1105	16-17	0.06166156903514519
1105	18-19	0.06384299732316379
1105	20-21	0.0865727781097334
1105	22-23	0.08716171815132157
1105	24-25	0.05468466697189456
1105	26-27	0.01894021172294913
1105	28-29	-6.376811407591276E-4
1105	30-31	-0.0064433017305916
1105	32-33	-0.025614611781790586
1105	34-35	-0.05576114276797739
1105	36-37	-0.01681990002919065
1105	38-39	-0.01680507774846518
1105	40-41	0.04355002036826505
1105	42-43	0.040412532718569594
1105	44-45	-0.036671979917358044
1105	46-47	-0.058321230917592004
1105	48-49	0.005155456090818689
1105	50-51	7.516722526901276E-5
1105	52-53	0.004299931611377872
1105	54-55	-0.04175351089008217
1105	56-57	0.005021549060010955
1105	58-59	-0.03073959269677573
1105	60-61	-0.01089443599450135
1105	62-63	-0.014802493565667874
1105	64-65	-0.057693392493298745
1105	66-67	-0.030915708572273104
1105	68-69	-0.02133043876452234
1105	70-71	0.023334196201687973
1105	72-73	0.02403670064951058
1105	74-75	0.015510717842396105
1105	76-77	0.04179348675008043
1105	78-79	0.023220630806818576
1105	80-81	0.00164964870607065
1105	82-83	-0.025089348101296594
1105	84-85	-0.04677453633431128
1105	86-87	-0.051139934846855084
1105	88-89	-0.033828913256236604
1105	90-91	-0.03671159810318514
1105	92-93	-0.01795411203057995
1105	94-95	-0.011936072166786715
1105	96-97	-0.05624728474355223
1105	98-99	-0.039393569805287854
1105	100-101	0.09318669701265136
1106	1	0.21301020415709004
1106	2	0.24169089957654322
1106	3	0.1116276821189146
1106	4	0.11912642163372311
1106	5	0.0637162812117964
1106	6	-0.026074761602956187
1106	7	0.0053429558908604236
1106	8	0.005624489047455938
1106	9	0.039934307958311877
1106	10-11	0.10858179032831572
1106	12-13	0.06932883772557119
1106	14-15	0.050350652941816065
1106	16-17	0.0513544779966395
1106	18-19	0.07936036565135396
1106	20-21	0.05668680714342145
1106	22-23	-0.02556582504489313
1106	24-25	-0.03431812077902663
1106	26-27	-0.05187441804591231
1106	28-29	-0.08000113958637911
1106	30-31	-0.052884122478438655
1106	32-33	-0.14958607191616125
1106	34-35	-0.08371481570640782
1106	36-37	-0.11149158258956504
1106	38-39	-0.13667534760281086
1106	40-41	-0.08759532463086117
1106	42-43	-0.09592051166973192
1106	44-45	-0.13897391094369738
1106	46-47	-0.17151450252838885
1106	48-49	-0.1387268804719568
1106	50-51	-0.143803765934571
1106	52-53	-0.17569349411225232
1106	54-55	-0.1863485326969183
1106	56-57	-0.1457380772406296
1106	58-59	-0.1509126879818936
1106	60-61	-0.17181765115437742
1106	62-63	-0.1569294347904986
1106	64-65	-0.11242433542930286
1106	66-67	-0.08670228643832445
1106	68-69	-0.13696272547395694
1106	70-71	-0.08054404781657354
1106	72-73	-0.07053391632983619
1106	74-75	-0.07145094323255563
1106	76-77	-0.11868915174120787
1106	78-79	-0.07645128100988074
1106	80-81	-0.04924181120514248
1106	82-83	-0.0841423790613618
1106	84-85	-0.1034978098767283
1106	86-87	-0.08546996975693233
1106	88-89	-0.06602806034558384
1106	90-91	-0.06099613097316947
1106	92-93	-0.07250626333522803
1106	94-95	-0.02061760575341154
1106	96-97	-0.046163499875788716
1106	98-99	-0.02848922730656156
1106	100-101	0.1024369610501985
1107	1	0.13538079647312884
1107	2	0.22068841509793558
1107	3	0.07223909952087126
1107	4	0.1822122629111007
1107	5	0.03739733574526127
1107	6	0.07730801342255234
1107	7	0.024321604161677612
1107	8	0.04308076068951294
1107	9	-0.027042035014424926
1107	10-11	0.09290634898139416
1107	12-13	0.044473090385110936
1107	14-15	0.07649859861636088
1107	16-17	0.07187489450660678
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2216	34-35	-0.4842776280897638
2216	36-37	-0.4408064265592273
2216	38-39	-0.435469623472855
2216	40-41	-0.4452353486509608
2216	42-43	-0.562160408834238
2216	44-45	-0.7178379318238157
2216	46-47	-0.6237113284710958
2216	48-49	-0.6253257047600798
2216	50-51	-0.7433335532119756
2216	52-53	-0.8394119133174271
2216	54-55	-0.7749744384934019
2216	56-57	-0.873016988917108
2216	58-59	-0.8415785341152286
2216	60-61	-0.8939457441352374
2216	62-63	-0.7663909888594773
2216	64-65	-0.8754802135560311
2216	66-67	-1.0571609076125839
2216	68-69	-0.8243873944022155
2216	70-71	-0.8295454358209184
2216	72-73	-0.7731349057740999
2216	74-75	-0.7970300832854988
2216	76-77	-0.7602793233520231
2216	78-79	-0.7646133542837745
2216	80-81	-0.7119729571364175
2216	82-83	-0.726604362250999
2216	84-85	-0.7028464549409463
2216	86-87	-0.7439046176207675
2216	88-89	-0.7638229135761563
2216	90-91	-0.6855346816464554
2216	92-93	-0.6935419229497981
2216	94-95	-0.7629455489767416
2216	96-97	-0.7915933057081332
2216	98-99	-0.7914195666362325
2216	100-101	-0.6268070956086049
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65800.0
3	20502.0
4	25424.0
5	42308.0
6	133488.0
7	142487.0
8	118870.0
9	154284.0
10	222787.0
11	225824.0
12	167191.0
13	109497.0
14	82293.0
15	71469.0
16	66693.0
17	66363.0
18	64518.0
19	67628.0
20	73159.0
21	80273.0
22	95495.0
23	119669.0
24	149060.0
25	180760.0
26	208455.0
27	236772.0
28	265189.0
29	298562.0
30	332214.0
31	397844.0
32	482999.0
33	605683.0
34	832735.0
35	1252261.0
36	2183001.0
37	4724196.0
38	7811394.0
39	3274107.0
40	7118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.972619811107926	19.236097360101745	14.576971008110714	37.21431182067961
2	19.36193882588493	21.167805177607118	50.241789431561756	9.2284665649462
3	13.87128301589063	22.71830164878671	46.3138640414999	17.096551293822763
4	18.776920811162753	28.908732891752415	38.93773386814547	13.376612428939355
5	18.586448407610774	32.203247359935865	38.43288504558034	10.777419186873018
6	16.479407635667524	34.1411064493453	37.068145597334386	12.311340317652796
7	14.355749266104093	17.516441323157437	55.70759580152254	12.420213609215933
8	14.24699658276237	21.08276058170639	48.38009781755787	16.290145017973373
9	16.51669907265923	22.04949154561536	45.94444300813632	15.489366373589085
10-11	17.78236998083606	29.572329506602955	38.60206873104934	14.043231781511636
12-13	18.345409677953818	23.0438657309536	42.98870285510684	15.62202173598575
14-15	19.185456552942128	25.253074906996282	41.41015678650982	14.151311753551768
16-17	19.500715667103506	26.12368754654744	39.36444956945708	15.011147216891965
18-19	20.16401315436409	27.116624316622502	36.30956588291525	16.409796646098158
20-21	21.170865681506456	26.923234138273834	36.09433941574038	15.811560764479331
22-23	21.479092939109037	28.70042350269799	34.157785036636255	15.662698521556711
24-25	20.783455282337172	29.01026370236629	33.046206267334846	17.160074747961694
26-27	20.880578037828382	29.432415089812665	32.772412784845464	16.914594087513493
28-29	21.61812969322982	29.086946794201562	32.497321242495424	16.79760227007319
30-31	21.310001170606448	31.094697030957285	30.34371673894315	17.25158505949312
32-33	21.135583349527966	29.6572559343108	31.408479415207108	17.79868130095413
34-35	21.976397243456013	30.1748424835939	30.20381951194854	17.644940761001543
36-37	22.32600517553376	30.500343846686924	30.0266872977954	17.14696367998392
38-39	22.60168126619304	28.243312156179755	31.43170464257	17.7233019350572
40-41	22.464522709531895	29.737831288042933	30.024125369145388	17.773520633279787
42-43	22.999215905855884	29.623781789177713	29.86261768289491	17.514384622071496
44-45	23.306006392555144	29.29715359329054	29.646629466927653	17.750210547226672
46-47	22.006325928075533	29.418711576634664	29.842320656115096	18.73264183917471
48-49	22.282569739527595	29.767162626429027	30.182389551048843	17.767878082994535
50-51	21.73474735085896	29.44996283942953	30.673395753031063	18.141894056680446
52-53	23.27150201623006	28.7293496324293	29.142992674524603	18.856155676816037
54-55	22.74397087226601	28.680187681875385	30.169046232431945	18.40679521342666
56-57	23.48131282350703	28.43453774699488	30.103453863522006	17.980695565976088
58-59	23.035664386656702	28.708216762196688	29.604201868233627	18.651916982912983
60-61	23.083295712431664	28.641709995798735	29.06090506446935	19.214089227300253
62-63	23.374666878344346	28.49461582731613	29.480840841435672	18.64987645290385
64-65	23.40657651356546	28.79247467348631	29.09591316687883	18.705035646069398
66-67	23.133052840352004	28.712950877415956	28.953728247046982	19.20026803518506
68-69	24.011029766248804	28.120032689551067	29.100477533813056	18.768460010387074
70-71	23.029039332380787	27.97895920630124	29.509705892073825	19.482295569244144
72-73	23.27948227086117	27.716336451001606	29.52896770174165	19.47521357639558
74-75	24.466591065624726	27.331049975149746	29.72179685091812	18.480562108307407
76-77	23.252696802930664	27.86443868607396	29.336954745616268	19.545909765379108
78-79	22.749294728553387	28.090754177471496	29.61626061468527	19.54369047928985
80-81	22.256734802925877	27.200540828372933	30.826739819750333	19.715984548950857
82-83	22.211750793012982	28.2740691218588	29.414689108182902	20.099490976945315
84-85	21.80492719456803	28.296209717179543	29.367903978199877	20.53095911005255
86-87	22.096557116052022	29.3019379818386	29.393544767691466	19.207960134417913
88-89	22.60931147985166	26.880315246371882	31.160953182415298	19.34942009136116
90-91	23.36081386190725	27.395324673242843	29.517074477443533	19.726786987406374
92-93	22.516580674317233	27.376334105415705	30.44282808402586	19.664257136241204
94-95	23.39214393735286	27.75436304619681	29.148373648403687	19.705119368046642
96-97	22.860134121396808	27.894708564665322	29.597336234140304	19.647821079797563
98-99	24.22052301449585	26.656070896421298	29.709106855895147	19.414299233187705
100-101	24.685887062745344	27.158594571250898	28.391528267944555	19.763990098059196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1364.0
1	2176.5
2	2281.0
3	1414.0
4	1248.5
5	1431.5
6	1902.0
7	2583.5
8	3535.5
9	4666.0
10	6122.0
11	7989.5
12	10135.5
13	13124.5
14	16618.5
15	20549.5
16	25614.0
17	32198.0
18	40334.0
19	49937.5
20	66573.5
21	90036.5
22	112463.0
23	135963.5
24	168928.0
25	220819.5
26	269926.0
27	379026.5
28	515224.0
29	659826.5
30	841654.5
31	877218.0
32	922086.5
33	1063088.5
34	1258062.0
35	1241960.5
36	1082922.0
37	1266712.5
38	1337376.5
39	1252208.0
40	1212116.5
41	1056132.5
42	866674.5
43	724045.0
44	673667.5
45	620384.5
46	516651.0
47	458059.0
48	461845.0
49	448715.5
50	395021.5
51	360539.0
52	368278.5
53	362674.5
54	379380.5
55	346850.0
56	278557.0
57	280468.5
58	260299.5
59	197917.5
60	146532.5
61	107836.0
62	78138.5
63	57784.0
64	43983.5
65	40092.0
66	34713.5
67	22813.5
68	13464.0
69	10312.0
70	8643.0
71	15510.0
72	40918.0
73	86746.0
74	112955.0
75	89695.5
76	57246.5
77	73198.5
78	80767.0
79	41463.5
80	13836.0
81	4153.0
82	1257.5
83	456.5
84	217.5
85	127.0
86	95.0
87	80.5
88	67.5
89	58.5
90	55.5
91	54.5
92	38.0
93	25.0
94	29.0
95	24.0
96	14.5
97	11.5
98	8.5
99	8.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31992226368598903
2	0.024616656556043726
3	0.031031049432383186
4	0.049795014386622996
5	0.04535639592350996
6	0.023285071017109815
7	0.024325985966423932
8	0.027939728431967292
9	0.028744964524832932
10-11	0.03232531915237943
12-13	0.059601218805350166
14-15	0.0748908846174453
16-17	0.05197307981830103
18-19	0.054553763296411886
20-21	0.06103689583921548
22-23	0.054622502962876025
24-25	0.08100085897087214
26-27	0.052338382045796175
28-29	0.05946766745336269
30-31	0.05615245153932074
32-33	0.07009089190777792
34-35	0.06885750589236421
36-37	0.057425117364142526
38-39	0.05116587973496499
40-41	0.06148272167599719
42-43	0.04889550675117796
44-45	0.057173726583930815
46-47	0.053294845404882926
48-49	0.04589452931240065
50-51	0.049958025595666526
52-53	0.05344803666157443
54-55	0.07021658729788378
56-57	0.05645687006223336
58-59	0.04854002447603484
60-61	0.058320697018646755
62-63	0.05509582466624339
64-65	0.05436914819219391
66-67	0.048938714541526845
68-69	0.04839468918122494
70-71	0.050470627108441975
72-73	0.04841236509545858
74-75	0.05088895707863802
76-77	0.04764640881200102
78-79	0.04560189473231045
80-81	0.039291593350902404
82-83	0.036255264083657826
84-85	0.04101794097438752
86-87	0.030428104357969162
88-89	0.028650692982253537
90-91	0.024707000117682308
92-93	0.0354107481813841
94-95	0.029982278521187452
96-97	0.022328607658023067
98-99	0.02634300417952884
100-101	0.02193384557347186
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.5458372E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.653908211095583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68154664769284	12.937013475666163
2	8.200542503761906	2.7314216427767746
3	3.552458515374558	1.7748695411631832
4	2.0458117275972603	1.362830429143506
5	1.3105933911312666	1.0913251018984302
6	0.9149611271228271	0.914260717669447
7	0.7283252423955754	0.8490623214275899
8	0.5559523088362739	0.7407022976884775
9	0.46549914243771834	0.6977141991451322
>10	3.5222803099199695	11.924269889486993
>50	0.5009372947182603	5.853882638504444
>100	0.41633679623875103	14.153512199094553
>500	0.05283136187653399	6.080854066934256
>1k	0.04226250126523145	13.825598964021685
>5k	0.005401666074526817	6.267726406552035
>10k+	0.004259463556565199	18.794956108827527
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	144868	0.5690387429329731	No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	110730	0.43494532957566967	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	99064	0.3891215039202035	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	92182	0.3620891390855629	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	92123	0.3618573882100552	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	91509	0.3594456079123991	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	80700	0.31698806192320544	No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC	80383	0.3157428919649693	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	79908	0.3138771010180855	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	76527	0.3005965974572137	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	66422	0.26090435005034884	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	66014	0.2593017338264992	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	65879	0.2587714563994901	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	62429	0.24521992215370253	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	58659	0.23041143400685635	No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG	58533	0.22991650840831457	No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT	57078	0.22420129613943893	No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC	55048	0.2162274948295987	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	54142	0.21266874409722664	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	51183	0.20104584849337578	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	50459	0.1982019902922308	No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT	50045	0.1965758061827363	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	48969	0.19234929869042688	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	44964	0.17661773502249084	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	44532	0.17492084725606177	No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA	43609	0.171295320847696	No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA	43427	0.17058042831646894	No Hit
ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT	43121	0.16937846614858168	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	41876	0.16448812987727573	No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT	39791	0.15629828961569106	No Hit
AGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG	38938	0.1529477218731818	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	38629	0.15173397576247216	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	35543	0.1396122265791387	No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA	34436	0.13526395167766422	No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG	33121	0.13009865674050172	No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT	32993	0.12959587518007828	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	32843	0.12900667803895707	No Hit
CTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAATTAGCAAGAG	32574	0.12795005116587974	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	32266	0.12674023303611087	No Hit
ATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTA	32192	0.12644956244649108	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	31405	0.1233582414460752	No Hit
CAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACC	30950	0.12157101011800754	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	30676	0.12049474334022617	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	30582	0.12012551313179021	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	30396	0.11939490867679992	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	29965	0.11770194889131166	No Hit
CTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTGTGACTAGA	29876	0.11735235858757977	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	29762	0.11690456876032765	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	28889	0.11347544139900226	No Hit
TTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTG	28659	0.11257200578261642	No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC	28110	0.11041554424611284	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	26947	0.10584730241195313	No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC	26207	0.10294059651575521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.855961881615997E-6	0.0	0.0	7.855961881615997E-6	0.0
2	7.855961881615997E-6	0.0	0.0	7.855961881615997E-6	0.0
3	1.1783942822423995E-5	0.0	0.0	7.855961881615997E-6	0.0
4	1.1783942822423995E-5	0.0	0.0	7.855961881615997E-6	0.0
5	1.1783942822423995E-5	0.0	0.0	7.855961881615997E-6	0.0
6	1.5711923763231994E-5	0.0	0.0	7.855961881615997E-6	0.0
7	1.9639904704039992E-5	0.0	0.0	7.855961881615997E-6	0.0
8	1.9639904704039992E-5	0.0	0.0	7.855961881615997E-6	0.0
9	1.9639904704039992E-5	0.0	0.0	7.855961881615997E-6	0.0
10-11	2.356788564484799E-5	0.0	0.0	7.855961881615997E-6	0.0
12-13	2.356788564484799E-5	0.0	0.0	7.855961881615997E-6	0.0
14-15	3.5351828467271986E-5	0.0	0.0	7.855961881615997E-6	0.0
16-17	4.320779034888798E-5	0.0	0.0	7.855961881615997E-6	0.0
18-19	6.284769505292798E-5	0.0	0.0	7.855961881615997E-6	0.0
20-21	7.463163787535197E-5	0.0	0.0	7.855961881615997E-6	0.0
22-23	1.1391144728343196E-4	0.0	0.0	7.855961881615997E-6	0.0
24-25	2.3567885644847991E-4	0.0	0.0	7.855961881615997E-6	0.0
26-27	3.4762631326150785E-4	0.0	0.0	7.855961881615997E-6	0.0
28-29	4.360058844296878E-4	0.0	0.0	7.855961881615997E-6	0.0
30-31	6.225849791180677E-4	0.0	0.0	7.855961881615997E-6	0.0
32-33	0.0010153830731988675	0.0	0.0	7.855961881615997E-6	0.0
34-35	0.0016202921380832993	0.0	0.0	7.855961881615997E-6	0.0
36-37	0.002460880059416211	0.0	0.0	1.1783942822423995E-5	0.0
38-39	0.0038160334839949703	0.0	0.0	1.3747933292827995E-5	0.0
40-41	0.005835015687570281	0.0	0.0	1.5711923763231994E-5	0.0
42-43	0.00826447189946003	0.0	0.0	1.9639904704039992E-5	0.0
44-45	0.011412748623517639	0.0	0.0	2.160389517444399E-5	0.0
46-47	0.015095230755525137	0.0	0.0	2.5531876115251988E-5	0.0
48-49	0.019514209313934133	0.0	0.0	2.7495866585655987E-5	0.0
50-51	0.02469718016533029	0.0	0.0	2.7495866585655987E-5	0.0
52-53	0.03070895499523693	0.0	0.0	2.7495866585655987E-5	0.0
54-55	0.03846671735333272	0.0	0.0	2.7495866585655987E-5	0.0
56-57	0.04759338106930011	0.0	0.0	2.7495866585655987E-5	0.0
58-59	0.05804770234326059	0.0	0.0	2.7495866585655987E-5	3.9279809408079985E-6
60-61	0.06960382227111772	0.0	0.0	2.7495866585655987E-5	3.9279809408079985E-6
62-63	0.08385650111483955	0.0	0.0	2.7495866585655987E-5	3.9279809408079985E-6
64-65	0.10153437933894595	0.0	0.0	2.7495866585655987E-5	3.9279809408079985E-6
66-67	0.12246855376298219	0.0	0.0	3.5351828467271986E-5	3.9279809408079985E-6
68-69	0.14759191986039014	0.0	0.0	3.5351828467271986E-5	3.9279809408079985E-6
70-71	0.17696339734528194	0.0	0.0	3.5351828467271986E-5	3.9279809408079985E-6
72-73	0.21145303399604656	0.0	0.0	3.7315818937675985E-5	5.891971411211998E-6
74-75	0.2543564058220219	0.0	0.0	3.9279809408079983E-5	7.855961881615997E-6
76-77	0.30648267689701447	0.0	0.0	3.9279809408079983E-5	7.855961881615997E-6
78-79	0.3684485402287311	0.0	0.0	3.9279809408079983E-5	7.855961881615997E-6
80-81	0.4387711830120167	0.0	0.0	3.9279809408079983E-5	7.855961881615997E-6
82-83	0.5193065762414031	0.0	0.0	3.9279809408079983E-5	7.855961881615997E-6
84-85	0.6265856277062807	3.9279809408079985E-6	0.0	3.9279809408079983E-5	7.855961881615997E-6
86-87	0.7534260242563822	3.9279809408079985E-6	0.0	3.9279809408079983E-5	7.855961881615997E-6
88-89	0.893943650442377	3.9279809408079985E-6	0.0	3.9279809408079983E-5	7.855961881615997E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	49420	0.0	87.94675	1
GCGTGTT	53170	0.0	81.16057	2
GTAAACG	19150	0.0	53.593166	1
TAAACGC	20760	0.0	49.438625	2
TCGAACG	3795	0.0	46.555405	2
GTCGAAC	4055	0.0	43.357876	1
CGTGTTT	102555	0.0	43.235317	1
CGAACGT	7285	0.0	42.83948	1
CGCAGGA	8645	0.0	40.123505	1
AAACGCT	29640	0.0	34.9641	3
CGCGTTT	1030	0.0	33.768993	1
ACGCTTC	31245	0.0	33.062172	5
GAACGTT	10015	0.0	31.725914	2
GTTTTTT	388745	0.0	31.263964	1
AACGCTT	33200	0.0	31.244164	4
TCGCAGG	5085	0.0	31.10228	2
CGCTTCG	34030	0.0	30.253983	6
AGTAAAC	13485	0.0	29.750456	1
CCTTTAG	12155	0.0	29.735983	5
ACGTTTA	11340	0.0	28.85762	4
>>END_MODULE