FastQCFastQC Report
Fri 27 May 2016
SRR2032143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25458372
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT3405411.3376385575636964No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2490430.9782361574416464No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT813340.31947840183967774No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA751870.295333102996531No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT688110.2702882965179392No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT681600.26773118092547316No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC672260.2640624467267585No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC644830.25328799500612215No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA597010.23450439014717828No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC559020.21958199055304872No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC556190.21847037194680005No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC521720.20493062164383488No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT460940.18105635348560387No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC459010.18029825316402792No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT443890.17435914598152624No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC429640.16876177314087484No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA425060.16696275786998477No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC392030.15398863682249594No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC384410.15099551534560027No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG371800.14604233137924136No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG371140.14578308463714806No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA354920.1394118995511575No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA352920.1386263033629959No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA351870.138213865364211No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG342980.13472189030783271No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC341940.1343133802899887No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT334900.13154808170765986No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG333810.1311199317851118No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT326100.12809145847974882No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC321400.12624530743756907No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA318260.12501192142215536No Hit
CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT314510.12353892856935235No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT312470.12273762045742752No Hit
CCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGC300780.11814581073762297No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA299750.11774122870071974No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG295100.11591471756324404No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG289890.11386823949308307No Hit
CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA289170.1135854248653449No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG288200.1132044107140865No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA283480.11135040371002515No Hit
CCCAAATAAATCTGTACTCAGAAAAAGCAAATCCATATGAATGCGGATTC281230.11046660799834333No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT277030.10881685600320397No Hit
GTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTA260370.10227283975581786No Hit
CCGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTA257720.10123192480650373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA557650.062.7163731
CCAAACG634800.055.339518
CCGAAAC634800.055.040732
CGAAACC718750.048.73033
CAAACGA746550.047.1013379
CGCTCGT14300.045.2172131
AAACCAA796150.044.58645
TCCGCTC23000.041.4925882
GAAACCA858550.041.1338844
ACTCGTC514150.040.87056742-43
CGCCTAT13400.040.8031771
CGCACGT5200.040.230021
ACCAAAC888550.040.025067
CTAACGA509550.039.9923694-95
TCGTCTA527250.039.86293444-45
CTCCGCT27600.038.4146351
CTCGCGT2000.038.0356561
GATATAT180150.037.9751555
TAGGTAG552600.037.12797576-77
AACCAAA971500.036.9348146
Produced by FastQC (version 0.11.5)