##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032143_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25458372
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46506952604825	34.0	31.0	34.0	31.0	34.0
2	32.602684609997844	34.0	31.0	34.0	31.0	34.0
3	32.72184364341915	34.0	31.0	34.0	31.0	34.0
4	36.18436324208005	37.0	37.0	37.0	35.0	37.0
5	36.19065900993198	37.0	37.0	37.0	35.0	37.0
6	36.10504183064023	37.0	36.0	37.0	35.0	37.0
7	36.0046594102718	37.0	36.0	37.0	35.0	37.0
8	35.951568387797934	37.0	36.0	37.0	35.0	37.0
9	37.74704403722281	39.0	38.0	39.0	35.0	39.0
10-11	37.86701433618772	39.0	38.0	39.0	35.0	39.0
12-13	37.78276091259881	39.0	38.0	39.0	35.0	39.0
14-15	39.230438458515735	41.0	39.0	41.0	36.0	41.0
16-17	39.19696626712816	41.0	39.0	41.0	36.0	41.0
18-19	39.204026616470216	41.0	39.0	41.0	36.0	41.0
20-21	39.18256183074079	41.0	39.0	41.0	36.0	41.0
22-23	39.140969992111046	41.0	39.0	41.0	36.0	41.0
24-25	39.12842030904412	41.0	39.0	41.0	36.0	41.0
26-27	39.0762605519316	40.5	39.0	41.0	36.0	41.0
28-29	39.04166986797113	40.0	39.0	41.0	36.0	41.0
30-31	38.95964372741509	40.0	38.5	41.0	35.0	41.0
32-33	38.900650756458425	40.0	38.0	41.0	35.0	41.0
34-35	38.81221627211669	40.0	38.0	41.0	35.0	41.0
36-37	38.70573850912383	40.0	38.0	41.0	35.0	41.0
38-39	38.4074452207706	40.0	38.0	41.0	34.0	41.0
40-41	38.170479223887526	40.0	38.0	41.0	33.5	41.0
42-43	38.158607687090125	40.0	38.0	41.0	33.0	41.0
44-45	38.154331451359106	40.0	38.0	41.0	33.0	41.0
46-47	38.0524857795306	40.0	38.0	41.0	33.0	41.0
48-49	38.09810754984647	40.0	37.5	41.0	33.5	41.0
50-51	38.387039457982624	40.0	38.0	41.0	34.0	41.0
52-53	38.344044230322346	40.0	38.0	41.0	34.0	41.0
54-55	38.227152466779884	40.0	38.0	41.0	34.0	41.0
56-57	38.11865992452306	40.0	37.0	41.0	33.0	41.0
58-59	37.95355500343855	40.0	37.0	41.0	33.0	41.0
60-61	37.77481401795841	39.5	37.0	41.0	33.0	41.0
62-63	37.50266281755958	39.0	36.0	41.0	33.0	41.0
64-65	37.15985381940369	39.0	35.5	41.0	32.0	41.0
66-67	36.8409678160096	38.5	35.0	40.0	32.0	41.0
68-69	36.389227854004176	37.0	35.0	40.0	31.5	41.0
70-71	36.00809407608625	37.0	35.0	39.0	31.0	41.0
72-73	35.662060382337096	36.5	35.0	39.0	31.0	41.0
74-75	35.34078402578138	36.0	34.5	39.0	31.0	40.5
76-77	35.247904893525785	36.0	35.0	37.5	31.0	40.0
78-79	34.91160683801776	35.0	34.5	37.0	31.0	39.0
80-81	34.549446111479554	35.0	34.0	37.0	31.0	39.0
82-83	34.238483827638305	35.0	34.0	36.0	31.0	39.0
84-85	33.987862303214044	35.0	34.0	36.0	30.5	37.0
86-87	33.71638443730809	35.0	34.0	36.0	30.0	37.0
88-89	33.586387004636435	35.0	34.0	35.0	30.0	37.0
90-91	33.49131081516131	35.0	34.0	35.0	30.5	36.0
92-93	33.371250958230945	35.0	34.0	35.0	30.5	36.0
94-95	33.24996533556819	35.0	34.0	35.0	30.0	36.0
96-97	33.143285968953556	35.0	34.0	35.0	30.0	36.0
98-99	33.043115345317446	35.0	34.0	35.0	30.5	36.0
100-101	32.27026779245743	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2776779337005806
1101	2	0.31058989954732397
1101	3	0.2567180261118054
1101	4	0.16619798892470072
1101	5	0.15436965443864636
1101	6	0.1557166146300517
1101	7	0.18119557841712464
1101	8	0.13080026899092445
1101	9	0.1686225670608934
1101	10-11	0.17334793901753898
1101	12-13	0.1945224634329108
1101	14-15	0.27116689591302645
1101	16-17	0.23934812212601742
1101	18-19	0.29205721803288043
1101	20-21	0.3079124618567306
1101	22-23	0.2503545682444255
1101	24-25	0.29978589661883603
1101	26-27	0.30160028859960164
1101	28-29	0.30867914182741174
1101	30-31	0.28298830886976134
1101	32-33	0.2832104998369189
1101	34-35	0.3004002856833736
1101	36-37	0.35685660677626885
1101	38-39	0.3449799588108604
1101	40-41	0.2849029206465019
1101	42-43	0.2494079252892405
1101	44-45	0.23175826951044343
1101	46-47	0.2722729420252108
1101	48-49	0.28097133773914607
1101	50-51	0.24323090336560682
1101	52-53	0.2731762835563458
1101	54-55	0.2688826046871
1101	56-57	0.2918669129308995
1101	58-59	0.2732616749503691
1101	60-61	0.25628348208751106
1101	62-63	0.27740475396140596
1101	64-65	0.23826887190698898
1101	66-67	0.24715597342532902
1101	68-69	0.2066158510080527
1101	70-71	0.24029390300491116
1101	72-73	0.22563101420954723
1101	74-75	0.22667959882783606
1101	76-77	0.26749408862453805
1101	78-79	0.30582284541614513
1101	80-81	0.31009343760405983
1101	82-83	0.3121296684121546
1101	84-85	0.2747703529399317
1101	86-87	0.3050733160457
1101	88-89	0.2883473385795128
1101	90-91	0.2807819301961487
1101	92-93	0.2921374392461047
1101	94-95	0.2868672929451108
1101	96-97	0.3095522054226336
1101	98-99	0.3177306955317505
1101	100-101	0.34432580287775494
1102	1	0.14770012814515354
1102	2	0.21351286078889586
1102	3	0.1427122630053148
1102	4	0.11916757309194992
1102	5	0.13204104239670045
1102	6	0.1371908867136895
1102	7	0.1185033164146958
1102	8	0.09722125250306846
1102	9	0.1484721936882707
1102	10-11	0.1533867077078881
1102	12-13	0.18016048712540567
1102	14-15	0.23798648679582612
1102	16-17	0.22507494666878358
1102	18-19	0.23916852855403903
1102	20-21	0.25977744462151975
1102	22-23	0.2562491656661976
1102	24-25	0.25961107881015977
1102	26-27	0.27996214002968145
1102	28-29	0.2665923467994702
1102	30-31	0.281617848537131
1102	32-33	0.26059616027797716
1102	34-35	0.302193737561403
1102	36-37	0.3056567081657846
1102	38-39	0.34365951923342664
1102	40-41	0.3188290427390825
1102	42-43	0.26157808517254466
1102	44-45	0.19959459906475985
1102	46-47	0.20057770173058032
1102	48-49	0.22608292206572145
1102	50-51	0.21166340573891063
1102	52-53	0.21243106029892544
1102	54-55	0.1744076930627685
1102	56-57	0.20574148160083894
1102	58-59	0.24633718065631172
1102	60-61	0.21109371541500366
1102	62-63	0.2207666604499252
1102	64-65	0.233446929880607
1102	66-67	0.22081961795915817
1102	68-69	0.23126483864499647
1102	70-71	0.24747721822688362
1102	72-73	0.22054415064311428
1102	74-75	0.1996966939365663
1102	76-77	0.22597579463032247
1102	78-79	0.27221023455842897
1102	80-81	0.27480032472544735
1102	82-83	0.2857103362379405
1102	84-85	0.2591670741460632
1102	86-87	0.2820722102746629
1102	88-89	0.2657762673584685
1102	90-91	0.2584483223862861
1102	92-93	0.26947926111273546
1102	94-95	0.26760351998468224
1102	96-97	0.28180988364901793
1102	98-99	0.26716383166754554
1102	100-101	0.34006476249882667
1103	1	0.2363191574870953
1103	2	0.27966481454964054
1103	3	0.18529297543437906
1103	4	0.14618120194084128
1103	5	0.11272172946792125
1103	6	0.10297236020428358
1103	7	0.09903371964603735
1103	8	0.09701189247651598
1103	9	0.13367142183088987
1103	10-11	0.1351116254374105
1103	12-13	0.15394498643724575
1103	14-15	0.19035820037316142
1103	16-17	0.19024078153592683
1103	18-19	0.18345031078857232
1103	20-21	0.17511907948460959
1103	22-23	0.1923154063356165
1103	24-25	0.22678716208849892
1103	26-27	0.22493186030837364
1103	28-29	0.19884435759936991
1103	30-31	0.2155058994226451
1103	32-33	0.2521572830047205
1103	34-35	0.30247807764839507
1103	36-37	0.26686614221464566
1103	38-39	0.2584685646103111
1103	40-41	0.22630585047278373
1103	42-43	0.20262407454487885
1103	44-45	0.17051260736040064
1103	46-47	0.20307131550746504
1103	48-49	0.20318481026243518
1103	50-51	0.19208670396112382
1103	52-53	0.21347664739062822
1103	54-55	0.21274029871147349
1103	56-57	0.2059810282450769
1103	58-59	0.20138912478427784
1103	60-61	0.20695777981023866
1103	62-63	0.21838596160577595
1103	64-65	0.2108254059265775
1103	66-67	0.22516767715151786
1103	68-69	0.2408760505940606
1103	70-71	0.22762539909530233
1103	72-73	0.1934433236944031
1103	74-75	0.1619797837613035
1103	76-77	0.1888727277777278
1103	78-79	0.2394376139038883
1103	80-81	0.2556377729137793
1103	82-83	0.2259327239490574
1103	84-85	0.23144338935004072
1103	86-87	0.23893386901898594
1103	88-89	0.23691589572387528
1103	90-91	0.22845958972013136
1103	92-93	0.22414011464227457
1103	94-95	0.24539808939720587
1103	96-97	0.2460743967700978
1103	98-99	0.2989317950467836
1103	100-101	0.3322955208335614
1104	1	0.1955011815239871
1104	2	0.2273176714373264
1104	3	0.16293194588239857
1104	4	0.10235497259356663
1104	5	0.07341880724109728
1104	6	0.09723944356144898
1104	7	0.10574829491586968
1104	8	0.0935832797302254
1104	9	0.11115653212635124
1104	10-11	0.09850388566889734
1104	12-13	0.06583383105644458
1104	14-15	0.16375871543107223
1104	16-17	0.14232461174960065
1104	18-19	0.1902135832103653
1104	20-21	0.18615103017486234
1104	22-23	0.16384619339700635
1104	24-25	0.20227573937233245
1104	26-27	0.1889742798504983
1104	28-29	0.1765713007788463
1104	30-31	0.18548466216218884
1104	32-33	0.17860191621331722
1104	34-35	0.2139504405700663
1104	36-37	0.20922168512955608
1104	38-39	0.14232401753796609
1104	40-41	0.2646210654505552
1104	42-43	0.18952168399388825
1104	44-45	0.16880438609903337
1104	46-47	0.16855423379270462
1104	48-49	0.1496109641827914
1104	50-51	0.18149888008314008
1104	52-53	0.18624505362500798
1104	54-55	0.17576085807537822
1104	56-57	0.18247382238743626
1104	58-59	0.16376854393927687
1104	60-61	0.16229266055995595
1104	62-63	0.16214740378721615
1104	64-65	0.156007481632912
1104	66-67	0.18378389092752911
1104	68-69	0.18079428929382146
1104	70-71	0.19597461240144298
1104	72-73	0.18657828615102545
1104	74-75	0.17342857454312366
1104	76-77	0.20572967925781427
1104	78-79	0.20798720633138146
1104	80-81	0.17740327310138326
1104	82-83	0.17364035912633824
1104	84-85	0.16921211461291819
1104	86-87	0.21284908416784987
1104	88-89	0.21439059416657358
1104	90-91	0.19721676166544455
1104	92-93	0.1935256160158687
1104	94-95	0.16568258653422419
1104	96-97	0.14601766371556835
1104	98-99	0.1918472698376874
1104	100-101	0.2772355129245554
1105	1	0.18336673184445118
1105	2	0.23722833382004183
1105	3	0.15808404195956172
1105	4	0.08746834075173382
1105	5	0.08392968148452695
1105	6	0.0608323949678109
1105	7	0.04089322504147219
1105	8	0.048852082844369704
1105	9	0.06358540646982647
1105	10-11	0.08019701233912002
1105	12-13	0.06844136655286093
1105	14-15	0.0994732779811045
1105	16-17	0.10982534492195128
1105	18-19	0.14075420623876056
1105	20-21	0.15349386000206522
1105	22-23	0.13528152742745903
1105	24-25	0.1813286145506794
1105	26-27	0.1394743322124583
1105	28-29	0.1330404439399473
1105	30-31	0.14157209999610387
1105	32-33	0.14549145114096262
1105	34-35	0.16246922351812287
1105	36-37	0.1329499075009295
1105	38-39	0.1976624204889177
1105	40-41	0.2570739814092633
1105	42-43	0.19171370539361732
1105	44-45	0.17441025253972953
1105	46-47	0.16283297491932558
1105	48-49	0.14759094133990658
1105	50-51	0.1340137227740854
1105	52-53	0.14162632879043002
1105	54-55	0.1274101672315382
1105	56-57	0.08334881270864969
1105	58-59	0.14847107304255758
1105	60-61	0.12969990433890644
1105	62-63	0.11083478610692055
1105	64-65	0.13760042995976818
1105	66-67	0.12070455240078815
1105	68-69	0.1628399886362928
1105	70-71	0.16337541324762128
1105	72-73	0.20447387595009303
1105	74-75	0.15957512586695088
1105	76-77	0.14151850038067693
1105	78-79	0.20140946113391323
1105	80-81	0.21909041631883497
1105	82-83	0.20202514755241907
1105	84-85	0.2355580651159883
1105	86-87	0.23953841257937114
1105	88-89	0.22736422529235512
1105	90-91	0.19844930207300138
1105	92-93	0.18073845519366927
1105	94-95	0.1846861598102052
1105	96-97	0.16495946271844275
1105	98-99	0.15689758810930243
1105	100-101	0.18960054200217513
1106	1	0.16224913332880675
1106	2	0.22833714744528777
1106	3	0.12439187620553582
1106	4	0.07518841106762864
1106	5	0.05962362657842846
1106	6	0.08326535697883486
1106	7	0.07788403649129805
1106	8	0.05018068335983372
1106	9	0.04703538586012712
1106	10-11	0.06434723783494434
1106	12-13	0.07181208942441941
1106	14-15	0.09550308457429679
1106	16-17	0.10495895633364682
1106	18-19	0.11408527637601651
1106	20-21	0.13458555392633542
1106	22-23	0.10038081047708403
1106	24-25	0.15457746171111353
1106	26-27	0.13904063802001332
1106	28-29	0.1450642381652969
1106	30-31	0.10436914884551385
1106	32-33	0.13902145441002034
1106	34-35	0.14757222268956838
1106	36-37	0.1644712406759865
1106	38-39	0.1541849078293538
1106	40-41	0.24346845232828684
1106	42-43	0.1849160383904973
1106	44-45	0.1889771912112863
1106	46-47	0.173999025025239
1106	48-49	0.17419118511140397
1106	50-51	0.11733273520733434
1106	52-53	0.1458112016038342
1106	54-55	0.15734329486193133
1106	56-57	0.18269856445753874
1106	58-59	0.156263170387696
1106	60-61	0.1528332127625731
1106	62-63	0.15562564450499394
1106	64-65	0.15657029539396916
1106	66-67	0.17691020249705502
1106	68-69	0.19110228158935882
1106	70-71	0.2055383588855193
1106	72-73	0.12528907539300604
1106	74-75	0.17308055794651267
1106	76-77	0.1973478344199222
1106	78-79	0.2102382434586687
1106	80-81	0.21177201564498915
1106	82-83	0.2324461771018349
1106	84-85	0.2148207534114377
1106	86-87	0.23934468539406595
1106	88-89	0.238297663894528
1106	90-91	0.19898819696344816
1106	92-93	0.21797249451186929
1106	94-95	0.23284301221512038
1106	96-97	0.24476521818772312
1106	98-99	0.2317921986579492
1106	100-101	0.33048143969347876
1107	1	0.1556254750706927
1107	2	0.17334745493429438
1107	3	0.10165954353775675
1107	4	0.054603308960160746
1107	5	0.036769916326633734
1107	6	0.015572190717840328
1107	7	0.03558942215170191
1107	8	0.030563667560969066
1107	9	0.020731697143396843
1107	10-11	0.047498780856209066
1107	12-13	0.032302305276452614
1107	14-15	0.07283093408072716
1107	16-17	0.09367949094724537
1107	18-19	0.07366611328837536
1107	20-21	0.11314079765821106
1107	22-23	0.09341836283146421
1107	24-25	0.12834932211297456
1107	26-27	0.1262227836945442
1107	28-29	0.07549410426419456
1107	30-31	0.11242477858412059
1107	32-33	0.11706095706963993
1107	34-35	0.13105180246486725
1107	36-37	0.12693210410490252
1107	38-39	0.022265647791279264
1107	40-41	0.20609067525710856
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2216	36-37	-0.7333258360809154
2216	38-39	-0.6944370119307379
2216	40-41	-0.7707129112095714
2216	42-43	-0.686483174151256
2216	44-45	-0.49722616215650106
2216	46-47	-0.41268056734145375
2216	48-49	-0.4660284644300461
2216	50-51	-0.7196932904117403
2216	52-53	-0.791051130150187
2216	54-55	-0.8553325275610817
2216	56-57	-0.7905495504057711
2216	58-59	-0.8164198836147634
2216	60-61	-0.8185129894898182
2216	62-63	-0.8413259685715531
2216	64-65	-0.8327697133458329
2216	66-67	-0.896076801828336
2216	68-69	-0.861449533284592
2216	70-71	-0.7910632981563523
2216	72-73	-0.8070801472516749
2216	74-75	-0.7967147046244847
2216	76-77	-0.7527088511710005
2216	78-79	-0.8478572624594847
2216	80-81	-0.6664359338697707
2216	82-83	-0.7108297353768336
2216	84-85	-0.7785980433561761
2216	86-87	-0.6370804888654291
2216	88-89	-0.6524807141757236
2216	90-91	-0.7862120007824274
2216	92-93	-0.7512324427909718
2216	94-95	-0.7869439766192556
2216	96-97	-0.7461256733902815
2216	98-99	-0.7726000021104085
2216	100-101	-0.46681096563631286
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1610.0
3	704.0
4	745.0
5	1187.0
6	2046.0
7	3743.0
8	5956.0
9	8003.0
10	10146.0
11	12663.0
12	15262.0
13	15782.0
14	17018.0
15	19141.0
16	20398.0
17	24369.0
18	23827.0
19	25409.0
20	28370.0
21	32581.0
22	39253.0
23	49003.0
24	63084.0
25	81576.0
26	106853.0
27	137393.0
28	177279.0
29	228758.0
30	296994.0
31	389695.0
32	527965.0
33	741561.0
34	1089380.0
35	1645811.0
36	2783191.0
37	5491010.0
38	8544279.0
39	2780706.0
40	15621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.45233442464476	10.011429190000214	5.6452945219187445	55.89094186343628
2	19.98354165512815	12.587974566947166	40.57373633103034	26.85474744689434
3	21.92012120806468	16.018930825584604	22.168762401617826	39.89218556473289
4	23.3837744303849	25.854804688521998	20.79992856609157	29.961492315001536
5	22.17522171049649	33.82573872791096	23.99819257811478	20.00084698347777
6	18.169937901472853	37.35878743069554	25.250819985770807	19.220454682060804
7	14.73835494066985	28.303942589250568	39.91022106501214	17.047481405067437
8	16.27032160579632	24.54016698318337	33.46303133601787	25.72648007500244
9	15.466684201173585	24.224596922379796	36.69970334316743	23.60901553327919
10-11	19.38734289865323	35.38367827586203	25.781157905946216	19.447820919538522
12-13	20.451370674728857	28.95164939740889	28.247580765108538	22.349399162753716
14-15	20.534696844158454	31.588843775943875	26.47992239357521	21.396536986322463
16-17	21.000759278775508	28.907913193185582	27.63654743017719	22.454780097861722
18-19	19.984467192167667	29.437593260087485	27.513196837566834	23.06474271017801
20-21	20.78777857437231	28.682216993293995	26.34575769416835	24.184246738165346
22-23	20.504842179224973	30.294007016630914	26.998332807769483	22.20281799637463
24-25	20.16769989325671	30.80758024650138	26.4862014413781	22.538518418863806
26-27	20.04452931880756	32.0726892697359	26.927591699723035	20.955189711733503
28-29	20.244868090574037	30.253639994381615	26.57742681694679	22.924065098097557
30-31	18.86745268720597	29.978090038515166	28.25990268078959	22.89455459348928
32-33	19.82911362310783	28.660707942976675	29.69823966904508	21.811938764870415
34-35	19.65005489447826	30.93821526747042	26.93732217037913	22.474407667672196
36-37	20.34747068914636	30.38838660964792	27.270808252050305	21.993334449155416
38-39	19.70099082534955	30.300113974767466	26.72353006837205	23.27536513151093
40-41	19.957794990499856	30.454283940531358	26.05739150086489	23.5305295681039
42-43	19.301543400052967	30.739288432805484	27.36698185514304	22.59218631199851
44-45	19.28044731218477	28.779444341531345	27.485317992839448	24.454790353444437
46-47	19.59849268284785	29.92664810597826	27.40735846099701	23.067500750176876
48-49	18.836395403482967	29.178733540676475	28.026113906314688	23.95875714952587
50-51	18.53960984946104	29.717227687428167	28.360522276267407	23.382640186843386
52-53	20.917808474500006	29.043271626017486	27.574565638975624	22.464354260506887
54-55	19.86549338519345	29.33229542394497	27.005963003512324	23.796248187349256
56-57	21.34630791831293	28.447283438085353	26.573515158813983	23.632893484787736
58-59	19.438435644172433	31.29255870606543	27.136626787853434	22.132378861908702
60-61	18.712917361232716	32.43981874389149	26.40172914638353	22.445534748492264
62-63	19.357157867654422	31.11486604591421	27.819401100228415	21.708574986202954
64-65	20.912658387256762	30.197581398140482	26.544050136825287	22.345710077777472
66-67	21.33980770724538	30.16819748594934	26.650808718997236	21.841186087808044
68-69	20.001752293290767	30.175281964651912	26.030210777589673	23.792754964467647
70-71	19.8581123746332	31.422467820887345	26.021143130228673	22.698276674250785
72-73	18.761982908609696	31.07287199314405	27.058658248909666	23.106486849336584
74-75	18.95584969279126	29.431341733488463	28.650149303212398	22.96265927050788
76-77	18.18587775354888	29.208579322529165	28.548998097893264	24.056544826028695
78-79	20.2222306434131	31.456483663122164	26.632327852965066	21.688957840499672
80-81	19.465861484214948	30.582328205518788	26.845159975962996	23.106650334303268
82-83	19.915984323589075	31.047872901218593	25.683985006128555	23.352157769063773
84-85	19.369594671814227	30.90905052214562	25.759596443929823	23.961758362110327
86-87	19.74633814014704	30.841937635330158	26.98071188104346	22.43101234347934
88-89	18.38951023400501	31.062372555865615	27.386865990754742	23.161251219374634
90-91	18.629766602772378	32.88340861558965	26.983602958564873	21.503221823073098
92-93	20.350658929246162	30.845839777189692	27.659272761358533	21.14422853220561
94-95	18.590598737133003	30.041491147872772	28.02428215002106	23.34362796497316
96-97	18.470996267801077	31.305286297698032	28.781197269740584	21.44252016476031
98-99	19.1836265899167	30.29777338994052	27.555085085967413	22.96351493417537
100-101	20.241867201119618	31.311677432865636	26.48848774358517	21.957967622429578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	81.5
2	96.5
3	99.0
4	113.5
5	114.0
6	117.5
7	114.5
8	121.5
9	149.5
10	166.5
11	205.0
12	278.5
13	408.5
14	571.5
15	789.5
16	1252.0
17	1950.5
18	3036.0
19	4969.0
20	9820.0
21	17457.5
22	26362.5
23	47886.0
24	92301.0
25	127582.0
26	130169.5
27	171088.5
28	256410.5
29	334266.0
30	406637.0
31	478482.5
32	559601.0
33	633526.0
34	721350.0
35	865188.0
36	1018644.0
37	1158769.0
38	1382898.5
39	1342020.5
40	1173986.0
41	1280239.5
42	1124908.0
43	857039.0
44	827113.5
45	824997.0
46	789311.5
47	782289.5
48	772991.0
49	787515.5
50	760293.5
51	638623.0
52	576580.5
53	536718.0
54	517450.0
55	500044.0
56	455523.0
57	387230.0
58	321613.5
59	299967.5
60	274228.5
61	213964.0
62	168299.5
63	132412.5
64	100837.5
65	80659.5
66	59314.5
67	41461.0
68	25249.5
69	17883.5
70	25002.5
71	49548.0
72	48007.5
73	99626.5
74	93200.0
75	10424.5
76	3969.5
77	2723.5
78	1025.0
79	515.0
80	200.5
81	99.5
82	74.5
83	35.0
84	20.0
85	17.0
86	12.0
87	4.5
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14041746267200433
2	6.363329124108957E-4
3	0.0
4	0.010503421035720587
5	0.013080176532890633
6	0.014384266205238888
7	0.009867088123309692
8	0.0
9	0.0
10-11	0.0014258570815133033
12-13	0.0010664468254293715
14-15	6.697207504077637E-4
16-17	7.855961881615995E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.8101415125837583E-4
26-27	3.4959030373191185E-4
28-29	0.010473961178664526
30-31	3.4959030373191185E-4
32-33	0.0015849403096160272
34-35	0.0035096509706119463
36-37	0.0012216020725912874
38-39	0.36055919050911817
40-41	0.5119573239011512
42-43	0.04925295301679149
44-45	0.0
46-47	0.043364909586520305
48-49	0.0104602132453717
50-51	1.7675914233635992E-4
52-53	0.0012392779868249234
54-55	5.675932459467557E-4
56-57	0.015281809850213518
58-59	0.034933498497075936
60-61	1.1783942822423994E-4
62-63	8.641558069777596E-5
64-65	0.0014062171768092634
66-67	2.140749612740359E-4
68-69	0.0011980341869464394
70-71	4.968895890122118E-4
72-73	0.002981337534073271
74-75	4.3993386537049585E-4
76-77	8.445159022737196E-5
78-79	0.02634300417952884
80-81	4.929616080714038E-4
82-83	0.0019797023941672313
84-85	3.947620845512038E-4
86-87	1.2373139963545195E-4
88-89	0.0247011081462711
90-91	0.03245494252342609
92-93	0.015295557783506346
94-95	0.027177700129450543
96-97	0.015480172887724322
98-99	4.713577128969598E-4
100-101	0.026718126359376006
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.5458372E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.255306534170058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.85799693527308	9.958471601754303
2	10.892161614666946	3.1054220527359604
3	4.726085489957556	2.0211539209811473
4	2.8306344945684248	1.614062496250737
5	1.8342097558080832	1.3073611158504719
6	1.3409636730632306	1.146950892642177
7	1.0155819791920844	1.0134202696773598
8	0.7846994230265034	0.8948904649943351
9	0.6479707788552452	0.8313319869989795
>10	4.827302101250667	13.853247596591567
>50	0.6273754495211157	6.2457040931281576
>100	0.49127100278104524	14.040044731721125
>500	0.06127536461143331	6.077891233700009
>1k	0.05151317321800096	14.96298187726858
>5k	0.0064806905080537075	6.458565641546743
>10k+	0.004478073698698056	16.468500024158384
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT	340541	1.3376385575636964	No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	249043	0.9782361574416464	No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT	81334	0.31947840183967774	No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA	75187	0.295333102996531	No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT	68811	0.2702882965179392	No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT	68160	0.26773118092547316	No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC	67226	0.2640624467267585	No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC	64483	0.25328799500612215	No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA	59701	0.23450439014717828	No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC	55902	0.21958199055304872	No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC	55619	0.21847037194680005	No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC	52172	0.20493062164383488	No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT	46094	0.18105635348560387	No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC	45901	0.18029825316402792	No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT	44389	0.17435914598152624	No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC	42964	0.16876177314087484	No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA	42506	0.16696275786998477	No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC	39203	0.15398863682249594	No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC	38441	0.15099551534560027	No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG	37180	0.14604233137924136	No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG	37114	0.14578308463714806	No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA	35492	0.1394118995511575	No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA	35292	0.1386263033629959	No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA	35187	0.138213865364211	No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG	34298	0.13472189030783271	No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC	34194	0.1343133802899887	No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT	33490	0.13154808170765986	No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG	33381	0.1311199317851118	No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT	32610	0.12809145847974882	No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC	32140	0.12624530743756907	No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA	31826	0.12501192142215536	No Hit
CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT	31451	0.12353892856935235	No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT	31247	0.12273762045742752	No Hit
CCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGC	30078	0.11814581073762297	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	29975	0.11774122870071974	No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG	29510	0.11591471756324404	No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG	28989	0.11386823949308307	No Hit
CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA	28917	0.1135854248653449	No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG	28820	0.1132044107140865	No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA	28348	0.11135040371002515	No Hit
CCCAAATAAATCTGTACTCAGAAAAAGCAAATCCATATGAATGCGGATTC	28123	0.11046660799834333	No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT	27703	0.10881685600320397	No Hit
GTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTA	26037	0.10227283975581786	No Hit
CCGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTA	25772	0.10123192480650373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.855961881615997E-6	0.0	0.0	7.855961881615997E-6	0.0
2	7.855961881615997E-6	0.0	0.0	7.855961881615997E-6	0.0
3	1.1783942822423995E-5	0.0	0.0	7.855961881615997E-6	0.0
4	1.1783942822423995E-5	0.0	0.0	1.5711923763231994E-5	0.0
5	1.1783942822423995E-5	0.0	0.0	1.5711923763231994E-5	0.0
6	1.5711923763231994E-5	0.0	0.0	1.5711923763231994E-5	0.0
7	1.9639904704039992E-5	0.0	0.0	1.5711923763231994E-5	0.0
8	1.9639904704039992E-5	0.0	0.0	1.5711923763231994E-5	0.0
9	1.9639904704039992E-5	0.0	0.0	1.5711923763231994E-5	0.0
10-11	2.7495866585655987E-5	0.0	0.0	2.356788564484799E-5	0.0
12-13	2.7495866585655987E-5	0.0	0.0	3.5351828467271986E-5	0.0
14-15	3.9279809408079983E-5	0.0	0.0	6.088370458252398E-5	0.0
16-17	4.517178081929198E-5	0.0	0.0	8.641558069777596E-5	0.0
18-19	6.284769505292798E-5	0.0	0.0	1.0212750446100795E-4	0.0
20-21	7.659562834575597E-5	0.0	0.0	1.4140731386908794E-4	0.0
22-23	1.1391144728343196E-4	0.0	0.0	1.944350565699959E-4	0.0
24-25	2.3567885644847991E-4	0.0	0.0	2.081829898628239E-4	0.0
26-27	3.4566232279110385E-4	0.0	0.0	2.710306849157519E-4	0.0
28-29	4.281499225480718E-4	0.0	0.0	2.965625610310039E-4	0.0
30-31	6.068730553548357E-4	0.0	0.0	3.1620246573504387E-4	0.0
32-33	9.937791780244235E-4	0.0	0.0	3.2405842761665986E-4	0.0
34-35	0.0015888682905568353	0.0	0.0	3.3191438949827586E-4	0.0
36-37	0.002435348183300959	0.0	0.0	3.4762631326150785E-4	0.0
38-39	0.0037787176650572944	0.0	0.0	3.7119419890635583E-4	0.0
40-41	0.005793771887691797	0.0	0.0	3.8101415125837583E-4	0.0
42-43	0.008219300118640737	0.0	0.0	3.888701131399918E-4	0.0
44-45	0.011371504823639154	0.0	0.0	4.006540559624158E-4	0.0
46-47	0.015026491089060998	0.0	0.0	4.144019892552438E-4	0.0
48-49	0.01943368570464757	1.9639904704039993E-6	0.0	4.242219416072638E-4	0.0
50-51	0.024555772851461204	3.9279809408079985E-6	0.0	4.242219416072638E-4	0.0
52-53	0.030479168110199663	3.9279809408079985E-6	0.0	4.261859320776678E-4	0.0
54-55	0.038091595173485565	3.9279809408079985E-6	0.0	4.340418939592838E-4	0.0
56-57	0.047067031623231836	3.9279809408079985E-6	0.0	4.517178081929198E-4	0.0
58-59	0.0573131699073295	3.9279809408079985E-6	0.0	4.7921367477857577E-4	0.0
60-61	0.06861986304544532	3.9279809408079985E-6	0.0	4.8510564618978777E-4	0.0
62-63	0.08237565230015494	3.9279809408079985E-6	0.0	5.106375223050398E-4	0.0
64-65	0.09948986525925538	3.9279809408079985E-6	0.0	5.263494460682718E-4	0.0
66-67	0.11975431893288385	3.9279809408079985E-6	0.0	5.381333888906958E-4	0.0
68-69	0.14397228542343554	3.9279809408079985E-6	0.0	5.420613698315038E-4	0.0
70-71	0.1721909005022002	3.9279809408079985E-6	0.0	5.459893507723118E-4	0.0
72-73	0.20511130876711203	3.9279809408079985E-6	0.0	5.479533412427158E-4	0.0
74-75	0.24612139377961795	3.9279809408079985E-6	0.0	5.990170934732197E-4	0.0
76-77	0.29611084322281095	3.9279809408079985E-6	0.0	6.500808457037238E-4	0.0
78-79	0.35543317538136376	3.9279809408079985E-6	0.0	7.148925312270557E-4	0.0
80-81	0.42247791806954504	3.9279809408079985E-6	0.0	7.325684454606916E-4	0.0
82-83	0.49890267924437587	3.9279809408079985E-6	0.0	7.404244073423076E-4	0.0
84-85	0.6009889399055054	3.9279809408079985E-6	0.0	7.502443596943277E-4	0.0
86-87	0.721933437063454	3.9279809408079985E-6	0.0	7.855961881615996E-4	0.0
88-89	0.8565826597238818	3.9279809408079985E-6	0.0	8.386239308625077E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGAAA	55765	0.0	62.716373	1
CCAAACG	63480	0.0	55.33951	8
CCGAAAC	63480	0.0	55.04073	2
CGAAACC	71875	0.0	48.7303	3
CAAACGA	74655	0.0	47.101337	9
CGCTCGT	1430	0.0	45.217213	1
AAACCAA	79615	0.0	44.5864	5
TCCGCTC	2300	0.0	41.492588	2
GAAACCA	85855	0.0	41.133884	4
ACTCGTC	51415	0.0	40.870567	42-43
CGCCTAT	1340	0.0	40.803177	1
CGCACGT	520	0.0	40.23002	1
ACCAAAC	88855	0.0	40.02506	7
CTAACGA	50955	0.0	39.99236	94-95
TCGTCTA	52725	0.0	39.862934	44-45
CTCCGCT	2760	0.0	38.414635	1
CTCGCGT	200	0.0	38.035656	1
GATATAT	18015	0.0	37.975155	5
TAGGTAG	55260	0.0	37.127975	76-77
AACCAAA	97150	0.0	36.934814	6
>>END_MODULE