FastQCFastQC Report
Fri 27 May 2016
SRR2032142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30225663
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1709910.5657146379220863No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1296670.4289963796658488No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1152130.3811760886766984No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG1099870.36388614535932595No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1060280.35078800422012246No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1043900.3453687682549759No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC1024430.33892722220849214No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC939720.3109013688136469No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA896070.2964599982471849No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA807490.2671537759155192No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA805030.26633989798668767No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG793940.26267083041321543No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA789950.26135076011401304No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT782090.2587503208779903No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT755190.24985059881068614No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG701580.23211401516651595No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA670340.22177842715972845No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT669310.2214376571326161No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT650870.21533688111324473No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC640860.2120251258012107No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG630180.2084917045492104No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG613710.2030426925622773No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT578290.19132417376584923No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT547540.18115069965545502No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA545740.18055517921972464No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC544300.18007876287114033No Hit
ATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATT511910.16936270347485843No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA498030.16477057922600408No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA487680.16134633672055432No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT473460.15664172527828424No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA461090.15254917650607036No Hit
CTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAATTAGCAAGAG434100.14361967841697965No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG433170.14331199285851892No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA432250.1430076157469234No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA430060.1422830658834514No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA418010.13829638741092298No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC409070.135338635913462No Hit
AGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG404870.1339490882300911No Hit
ATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTA396010.1310178043075515No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT390340.12914191493500077No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA386250.12778876016714671No Hit
CAAGTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACC386150.12775567569849502No Hit
CTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTGTGACTAGA368550.12193280921579785No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC366620.12129427897082025No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA364370.12054987842615728No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC356950.11809501085220198No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT355190.11751272420393226No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG353780.11704623319594347No Hit
TTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTG353600.11698668115237042No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA352000.1164573296539434No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG329010.10885121031092022No Hit
ATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTT326210.10792484518867296No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT322210.10660146644260542No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA322080.10655845663335821No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT318130.10525162012161651No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG315950.10453037870500972No Hit
ATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAA308410.10203580976867241No Hit
ATATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAA308370.10202257598121173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT489350.087.4057161
GCGTGTT530050.080.1237642
GTAAACG195850.052.1103631
TAAACGC214950.047.4613762
CGAACGT72950.046.8950921
TCGAACG36950.044.6024322
CGCGTTT8450.043.9811021
CGTGTTT1040450.043.320971
GTCGAAC40700.041.0904581
CGCAGGA110200.039.4313351
GAACGTT100000.034.7659872
AAACGCT308000.033.5552943
CCTTTAG139500.032.788335
ACGCTTC321850.031.9648135
GTTTTTT3989000.031.091261
AACGCTT336150.030.661334
CGCTTCG349950.029.2987086
GTGTTTT1508800.028.8845272
TCGCAGG72300.028.8383052
AGTAAAC150550.028.6731661
Produced by FastQC (version 0.11.5)